WO2013048035A3 - 마이크로 rna를 이용하여 식물의 환경 스트레스에 대한 내성을 증가시키는 방법 - Google Patents

마이크로 rna를 이용하여 식물의 환경 스트레스에 대한 내성을 증가시키는 방법 Download PDF

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WO2013048035A3
WO2013048035A3 PCT/KR2012/007292 KR2012007292W WO2013048035A3 WO 2013048035 A3 WO2013048035 A3 WO 2013048035A3 KR 2012007292 W KR2012007292 W KR 2012007292W WO 2013048035 A3 WO2013048035 A3 WO 2013048035A3
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resistance against
microrna
plant
drought stress
mir399
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PCT/KR2012/007292
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French (fr)
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WO2013048035A2 (ko
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김민균
장재영
박상령
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서울대학교산학협력단
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Publication of WO2013048035A2 publication Critical patent/WO2013048035A2/ko
Publication of WO2013048035A3 publication Critical patent/WO2013048035A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 애기장대(Arabidopsis thaliana) 유래 miR399(microRNA 399) 유전자를 포함하는 재조합 벡터를 식물세포에 형질전환시켜 miR399 유전자를 과발현하는 단계를 포함하는 식물의 건조 스트레스에 대한 내성을 증가시키는 방법, 애기장대 유래 miR399 유전자를 포함하는 재조합 벡터를 이용한 야생형에 비해 건조 스트레스에 대한 내성이 증가된 식물체의 제조 방법, 상기 방법에 의해 제조된 야생형에 비해 건조 스트레스에 대한 내성이 증가된 형질전환 식물체 및 이의 종자 및 애기장대 유래 miR399 유전자를 포함하는, 식물체의 건조 스트레스에 대한 내성 증가용 조성물에 관한 것이다.
PCT/KR2012/007292 2011-09-28 2012-09-11 마이크로 rna를 이용하여 식물의 환경 스트레스에 대한 내성을 증가시키는 방법 WO2013048035A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110098166A KR101350167B1 (ko) 2011-09-28 2011-09-28 식물의 건조 스트레스에 대한 내성을 증가시키는 ｍｉＲ399 유전자 및 이의 용도
KR10-2011-0098166 2011-09-28

Publications (2)

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WO2013048035A2 WO2013048035A2 (ko) 2013-04-04
WO2013048035A3 true WO2013048035A3 (ko) 2013-08-22

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PCT/KR2012/007292 WO2013048035A2 (ko) 2011-09-28 2012-09-11 마이크로 rna를 이용하여 식물의 환경 스트레스에 대한 내성을 증가시키는 방법

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KR (1) KR101350167B1 (ko)
WO (1) WO2013048035A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101363412B1 (ko) * 2012-10-08 2014-03-19 서울대학교산학협력단 고추로부터 분리된 ｍｉｃｒｏＲＮＡ－３９６ 분자의 새로운 표적 유전자인 ＤＲＭ 메틸전달효소의 발굴 및 이의 용도
KR101595866B1 (ko) * 2014-03-31 2016-02-19 서울대학교산학협력단 식물의 가뭄 스트레스에 대한 내성을 증가시키는 벼 유래 miR399f 전구체 DNA 및 이의 용도
CN115633638B (zh) * 2022-09-21 2023-12-12 广西科学院 一种丽花石山苣苔的组织培养方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 9 August 2005 (2005-08-09), "Arabidopsis thaliana MIR399c microRNA precursor transcript, partial sequence", accession no. Q063659 *
FRAZIER, T.P. ET AL.: "Salt and Drought Stresses Induce the Aberrant Expression of microRNA Genes in Tobacco", MOL. BIOTECHNOL., vol. 49, 27 February 2011 (2011-02-27), pages 159 - 165, XP019951516, DOI: doi:10.1007/s12033-011-9387-5 *
WANG, T. ET AL.: "Identification of drought-responsive microRNAs in Medicago truncatula by genome-wide high-throughput sequencing", BMC GENOMICS, vol. 12, 15 July 2011 (2011-07-15), pages 367, XP021104721, DOI: doi:10.1186/1471-2164-12-367 *
ZHANG, X. ET AL.: "Over-expression of microRNA169 confers enhanced drought tolerance to tomato", BIOTECHNOL. LETT., vol. 33, 20 October 2010 (2010-10-20), pages 403 - 409, XP019873600, DOI: doi:10.1007/s10529-010-0436-0 *

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WO2013048035A2 (ko) 2013-04-04
KR101350167B1 (ko) 2014-01-13
KR20130034258A (ko) 2013-04-05

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