WO2013034242A1 - Dispositif et procédé de filtration d'un échantillon de liquide - Google Patents

Dispositif et procédé de filtration d'un échantillon de liquide Download PDF

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Publication number
WO2013034242A1
WO2013034242A1 PCT/EP2012/003474 EP2012003474W WO2013034242A1 WO 2013034242 A1 WO2013034242 A1 WO 2013034242A1 EP 2012003474 W EP2012003474 W EP 2012003474W WO 2013034242 A1 WO2013034242 A1 WO 2013034242A1
Authority
WO
WIPO (PCT)
Prior art keywords
filter
sample container
sample
liquid sample
container
Prior art date
Application number
PCT/EP2012/003474
Other languages
German (de)
English (en)
Inventor
Franziska Jonas
Bernhard Diel
Original Assignee
Sartorius Stedim Biotech Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sartorius Stedim Biotech Gmbh filed Critical Sartorius Stedim Biotech Gmbh
Publication of WO2013034242A1 publication Critical patent/WO2013034242A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4005—Concentrating samples by transferring a selected component through a membrane
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B01D63/087—Single membrane modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/24—Specific pressurizing or depressurizing means
    • B01D2313/243—Pumps
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G01N2001/1418—Depression, aspiration
    • G01N2001/1427—Positive displacement, piston, peristaltic

Definitions

  • the invention relates to a device for filtering a liquid sample with a pressurizable
  • Sample container for receiving the liquid sample and with a connectable to a connection of the sample container filter attachment, a filter and to the his
  • the invention further relates to a method for filtering a liquid sample, wherein in a first step, the liquid sample with a negative pressure in a
  • Sample container is filled, in a second step, the sample container is connected to a filter attachment, and in a third step by overpressure the liquid sample is fed to the filter.
  • Sample container such as a syringe
  • This filter attachment has a filter and a filtrate connection facing away from the sample container on its filtrate side.
  • Liquid samples containing, for example, mammalian cells or microorganisms and a high proportion of
  • Particulate substances are usually supplied for particle-free clarification of a centrifugation or sedimentation and / or direct filtration.
  • Object of the present invention is therefore, in particular an apparatus and a method for the filtration of
  • the object with respect to the device is achieved in conjunction with the preamble of claim 1, characterized in that the sample container contains a filter aid suitable for precoat filtration, and in that the filter attachment has a filter facing the sample container
  • the filter attachment now has a
  • Suspension can absorb.
  • the sample container is partially filled with the filter aid before receiving the liquid sample. It is not
  • liquid sample is suspended in the submitted filter aid, a dry powder mass.
  • Invention is the sample container with kieselguhr, perlite,
  • Adsorbent or partially filled with a combination of the aforementioned materials as a filter aid.
  • Diatomaceous earth is subject to special requirements for the protection of the user, because certain components can be hazardous to health. Therefore, appropriate precautions are usually required when handling these substances.
  • Sample container which is already pre-filled, ensures dust-free work safely.
  • Hose connection with a valve and a sterile connector arranged to fill the sample container makes it possible to use storage tanks, such as bioreactors
  • Hose connection via the sterile connector can be separated sterile from the reservoir.
  • Invention is the sample container as a syringe
  • the filter is as a pulp-based depth filter, as a membrane filter, as a plastic fleece or a
  • a filter aid suitable for precoat filtration is supplied to the sample container and suspended with the liquid sample.
  • the filter aid is suspended with the liquid sample in the sample container by shaking. Without further aids is by simply shaking the sample container a
  • turbid solutions are filtered by the precoat filtration; in particular, by the precoat filtration, cell separation can be effected simply, quickly and safely.
  • Invention is for filling the sample container with the liquid sample of the sample container via a
  • Hose connection which has a valve and connected to a reservoir sterile connector, connected to the filter attachment, wherein the negative pressure
  • Hose connection via the sterile connector can be separated sterile from the reservoir.
  • Figure 1 a side view of a reservoir, to the for taking a liquid sample
  • Sample container is connectable (in a reduced scale);
  • Figure 2 a side view of an apparatus for
  • Figure 3 a side view corresponding to Figure 2 with
  • Figure a side view corresponding to Figure 2 with
  • Figure 5 a side view of an apparatus for
  • a device 1 for filtering a liquid sample 2 consists essentially of a sample container 3 and a filter attachment 4.
  • the sample container 3 is formed in the embodiments of Figures 1 to 3 as a syringe 5.
  • the syringe 5 has in known manner a syringe body 6, in which a piston 7 is guided longitudinally displaceable. At its end facing the filter attachment 4, the syringe body 6 has a syringe port 8.
  • the filter attachment 4 has at its end facing the sample container 3 a with the syringe port. 8
  • the filter attachment 4 has a
  • the filter attachment 4 has a housing lower part 11 carrying the filtrate connection 10, in which a filter 14, which is laterally sealed, rests on the support surface 13 which has filtrate channels 12.
  • the filter 14 may be used as a pulp or polymer based depth filter, as a membrane filter, as a
  • the housing lower part 11 or the filter attachment 4 has a dead space 15.
  • the syringe port 8 To the syringe port 8 is the
  • Dead space 15 covered by a housing upper part 16.
  • Dead space 15 communicates via an inflow channel 17 with the connection 9 in connection.
  • the dead space 15 has a volume, the so is that he measures the dry matter of a
  • Filter aid 18 and the particles to be removed from the liquid sample 2 can be used as alluvial cake 19.
  • kieselguhr is used as filter aid 18.
  • the sample container has a
  • This embodiment allows the implementation of a method in which in a first step, the sample container (3) via the hose connection (23), which the valve (24) and one with the
  • Sample container (3) is filled, in a third step, the hose connection (23) and the valve (24) by
  • Reservoir (21) are separated sterile and in a fourth step by positive pressure, the liquid sample (2) is fed to the filter (14), wherein prior to the first step the sample container (3) for a precoat
  • Reservoir (21) for example, a bioreactor with cell cultures, via the sterile connector (20) and the
  • Hose connection (23) with open valve (24) to remove a cell-containing liquid sample (2) from the reservoir (21) without any risk of contamination for the Liquid sample (2) consists of microorganisms from the environment, since after removal of the liquid sample (2) a pre-sterilized arrangement of sample container (3),
  • Reservoir (21) can be separated.
  • Sample container 3 for example the syringe 5
  • a sterile connector 20 for example, via a sterile connector 20 with a
  • Sample container 3 with the filter attachment 4 is the
  • Liquid sample 2 and filter aid 18 by pressure, namely by pushing the piston 7 in the syringe body 6, pressed through the filter 14.
  • the precoat cake 19 deposits on the filter 14 towards the side of the unfiltrate.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hydrology & Water Resources (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un procédé et un dispositif de filtration d'un échantillon de liquide, suivant lesquels, dans une première étape, l'échantillon de liquide est introduit dans un contenant d'échantillon par une dépression, dans une deuxième étape, le contenant d'échantillon est relié à un raccord de filtre, et, dans une troisième étape, l'échantillon de fluide est amené au filtre par une surpression, avant le remplissage du contenant d'échantillon par l'échantillon de liquide, un auxiliaire de filtration approprié pour une filtration sur précouche est amené dans le contenant d'échantillon et mis en suspension avec l'échantillon de liquide.
PCT/EP2012/003474 2011-09-05 2012-08-16 Dispositif et procédé de filtration d'un échantillon de liquide WO2013034242A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102011112291.9 2011-09-05
DE102011112291.9A DE102011112291B4 (de) 2011-09-05 2011-09-05 Vorrichtung und Verfahren zur Filtration einer Flüssigkeitsprobe

Publications (1)

Publication Number Publication Date
WO2013034242A1 true WO2013034242A1 (fr) 2013-03-14

Family

ID=46750279

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/003474 WO2013034242A1 (fr) 2011-09-05 2012-08-16 Dispositif et procédé de filtration d'un échantillon de liquide

Country Status (2)

Country Link
DE (1) DE102011112291B4 (fr)
WO (1) WO2013034242A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102018103634B3 (de) 2018-02-19 2019-07-04 Sartorius Stedim Biotech Gmbh Medienfiltrationsvorrichtung und Verfahren zur Medienfiltration
CN112414820B (zh) * 2020-12-08 2022-05-31 西南石油大学 一种模拟页岩的厚泥饼制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2455130A (en) * 1945-11-08 1948-11-30 Mary P Lomax Method of and apparatus for filtering
WO2002034352A1 (fr) * 2000-10-23 2002-05-02 Usf Consumer & Commercial Watergroup, Inc. Cartouche de filtre d'eau dotee d'un tube d'alimentation a debit proportionnel
DE20301787U1 (de) 2002-02-15 2003-04-17 Sartorius AG, 37075 Göttingen Filtereinheit mit einer Laserstrahlschweißverbindung
WO2005093045A2 (fr) * 2004-03-15 2005-10-06 Purdue Research Foundation Concentration de cellules et recuperation de pathogenes
WO2010078404A1 (fr) * 2008-12-31 2010-07-08 3M Innovative Properties Company Procédés, trousses et systèmes de traitement d'échantillons

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH621363A5 (fr) * 1976-05-18 1981-01-30 Mueller Hans Dr Ing Fa
DE4110252C1 (fr) * 1990-06-02 1992-02-27 Schenk-Filterbau Gmbh, 7076 Waldstetten, De
DE4125594A1 (de) * 1990-12-22 1992-06-25 Schenk Filterbau Gmbh Verfahren zur herstellung von stabilisier- und/oder filterhilfsmitteln zur behandlung von fluessigkeiten, insbesondere getraenken
DE4237714C2 (de) * 1992-11-09 1996-02-29 Isp Investments Inc Crossflow-Anschwemmfiltrationsverfahren
US5801051A (en) * 1994-02-10 1998-09-01 Filtrox-Werk Ag Method and apparatus for cleaning a filter aid
JP3517359B2 (ja) * 1998-09-14 2004-04-12 テルモ株式会社 細胞分離・回収装置および細胞の分離・回収方法
GB2352652A (en) * 1999-08-06 2001-02-07 Fsm Technologies Ltd Pre-treating hollow fibre membranes for micro-organism detection
GB0011443D0 (en) * 2000-05-13 2000-06-28 Dna Research Instr Limited Separation device
DE10054632B4 (de) * 2000-11-03 2007-07-26 Michael Prof. Dr.med. Giesing Verfahren zur Isolierung von disseminierten Krebszellen aus einer zellhaltigen Körperflüssigkeit und Verwendung einer Filtervorrichtung in einem solchen Verfahren

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2455130A (en) * 1945-11-08 1948-11-30 Mary P Lomax Method of and apparatus for filtering
WO2002034352A1 (fr) * 2000-10-23 2002-05-02 Usf Consumer & Commercial Watergroup, Inc. Cartouche de filtre d'eau dotee d'un tube d'alimentation a debit proportionnel
DE20301787U1 (de) 2002-02-15 2003-04-17 Sartorius AG, 37075 Göttingen Filtereinheit mit einer Laserstrahlschweißverbindung
WO2005093045A2 (fr) * 2004-03-15 2005-10-06 Purdue Research Foundation Concentration de cellules et recuperation de pathogenes
WO2010078404A1 (fr) * 2008-12-31 2010-07-08 3M Innovative Properties Company Procédés, trousses et systèmes de traitement d'échantillons

Also Published As

Publication number Publication date
DE102011112291A1 (de) 2013-03-07
DE102011112291B4 (de) 2015-07-09

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