WO2012158207A3 - Method for mutiplexed microfluidic bead-based immunoassay - Google Patents

Method for mutiplexed microfluidic bead-based immunoassay Download PDF

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Publication number
WO2012158207A3
WO2012158207A3 PCT/US2012/000237 US2012000237W WO2012158207A3 WO 2012158207 A3 WO2012158207 A3 WO 2012158207A3 US 2012000237 W US2012000237 W US 2012000237W WO 2012158207 A3 WO2012158207 A3 WO 2012158207A3
Authority
WO
WIPO (PCT)
Prior art keywords
particles
intensity
particle
multiplexing
detection
Prior art date
Application number
PCT/US2012/000237
Other languages
French (fr)
Other versions
WO2012158207A2 (en
Inventor
Harold E. Ayliffe
Original Assignee
E.I. Spectra, Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E.I. Spectra, Llc filed Critical E.I. Spectra, Llc
Priority to US13/629,784 priority Critical patent/US9452429B2/en
Publication of WO2012158207A2 publication Critical patent/WO2012158207A2/en
Publication of WO2012158207A3 publication Critical patent/WO2012158207A3/en
Priority to US15/159,652 priority patent/US20170023560A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/12—Coulter-counters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
    • G01N2015/1019—

Abstract

A method for performing multiplexed bead-based immunoassays using a microfluidic cassette capable of detecting a particle passing in substantially single file through an interrogation zone and generating a Coulter effect signal responsive to a characteristic of the particle. A fluid sample may be prepared by associating antibody-coated beads of different sizes to particles of interest. A first multiplexing option may be based on bead size, in which case the intensity of the Coulter signal is used to sort or characterize the particles. A second multiplexing option may be based on detection of Stokes' shift phenomena, or even simply emission intensity, in which case particles may be characterized responsive to intensity of the signal resulting from detection of radiation. The first and second multiplexing options may be employed together to populate an array of particle characteristics.
PCT/US2012/000237 2006-02-02 2012-05-07 Method for mutiplexed microfluidic bead-based immunoassay WO2012158207A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US13/629,784 US9452429B2 (en) 2006-02-02 2012-09-28 Method for mutiplexed microfluidic bead-based immunoassay
US15/159,652 US20170023560A1 (en) 2006-02-02 2016-05-19 Method for mutiplexed microfluidic bead-based immunoassay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161486198P 2011-05-13 2011-05-13
US61/486,198 2011-05-13

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US12/985,536 Continuation-In-Part US8616048B2 (en) 2006-02-02 2011-01-06 Reusable thin film particle sensor

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/629,784 Continuation-In-Part US9452429B2 (en) 2006-02-02 2012-09-28 Method for mutiplexed microfluidic bead-based immunoassay

Publications (2)

Publication Number Publication Date
WO2012158207A2 WO2012158207A2 (en) 2012-11-22
WO2012158207A3 true WO2012158207A3 (en) 2014-04-24

Family

ID=47177533

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/000237 WO2012158207A2 (en) 2006-02-02 2012-05-07 Method for mutiplexed microfluidic bead-based immunoassay

Country Status (1)

Country Link
WO (1) WO2012158207A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU230593B1 (en) * 2013-02-05 2017-02-28 NORMA Instruments Zártkörűen Működő Részvénytársaság Measuring unit for determining physical characteristics of liquid samples
JP2014215041A (en) * 2013-04-22 2014-11-17 株式会社堀場製作所 Particle counter and manufacturing method for the same
CN106226223A (en) * 2016-08-24 2016-12-14 桂林优利特医疗电子有限公司 Particle analyzer

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352558A (en) * 1979-10-25 1982-10-05 Gesellschaft fur Strahlen-und Umweltforschung mbH, Munchen Apparatus for measuring particle characteristics
US5326692A (en) * 1992-05-13 1994-07-05 Molecular Probes, Inc. Fluorescent microparticles with controllable enhanced stokes shift
US6159748A (en) * 1995-03-13 2000-12-12 Affinitech, Ltd Evaluation of autoimmune diseases using a multiple parameter latex bead suspension and flow cytometry
US20090189088A1 (en) * 2006-02-02 2009-07-30 E. I. Spectra, Llc Fluorescence-activated cell detector
US20090272179A1 (en) * 2006-05-05 2009-11-05 E.I. Spectra, Llc Thin film particle sensor
US20090325217A1 (en) * 2008-06-30 2009-12-31 Microbix Biosystems Inc. Method and apparatus for sorting cells
US20100217172A1 (en) * 2009-02-25 2010-08-26 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Device, system, and method for controllably reducing inflammatory mediators in a subject

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4352558A (en) * 1979-10-25 1982-10-05 Gesellschaft fur Strahlen-und Umweltforschung mbH, Munchen Apparatus for measuring particle characteristics
US5326692A (en) * 1992-05-13 1994-07-05 Molecular Probes, Inc. Fluorescent microparticles with controllable enhanced stokes shift
US5326692B1 (en) * 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US6159748A (en) * 1995-03-13 2000-12-12 Affinitech, Ltd Evaluation of autoimmune diseases using a multiple parameter latex bead suspension and flow cytometry
US20090189088A1 (en) * 2006-02-02 2009-07-30 E. I. Spectra, Llc Fluorescence-activated cell detector
US20090272179A1 (en) * 2006-05-05 2009-11-05 E.I. Spectra, Llc Thin film particle sensor
US20090325217A1 (en) * 2008-06-30 2009-12-31 Microbix Biosystems Inc. Method and apparatus for sorting cells
US20100217172A1 (en) * 2009-02-25 2010-08-26 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Device, system, and method for controllably reducing inflammatory mediators in a subject

Also Published As

Publication number Publication date
WO2012158207A2 (en) 2012-11-22

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