WO2015195404A8 - Methods for detection of an analyte by movement of tethered microparticles - Google Patents

Methods for detection of an analyte by movement of tethered microparticles Download PDF

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Publication number
WO2015195404A8
WO2015195404A8 PCT/US2015/034815 US2015034815W WO2015195404A8 WO 2015195404 A8 WO2015195404 A8 WO 2015195404A8 US 2015034815 W US2015034815 W US 2015034815W WO 2015195404 A8 WO2015195404 A8 WO 2015195404A8
Authority
WO
WIPO (PCT)
Prior art keywords
detection
tethered
analyte
beads
molecules
Prior art date
Application number
PCT/US2015/034815
Other languages
French (fr)
Other versions
WO2015195404A1 (en
Inventor
Jonathan E. Silver
Keir C. NEUMAN
Original Assignee
The United States Of America, As Represented By The Secretary, Department Of Health And Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The United States Of America, As Represented By The Secretary, Department Of Health And Human Services filed Critical The United States Of America, As Represented By The Secretary, Department Of Health And Human Services
Publication of WO2015195404A1 publication Critical patent/WO2015195404A1/en
Publication of WO2015195404A8 publication Critical patent/WO2015195404A8/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

An immune sandwich assay in which read-out is based on counting micron-size beads tethered by polymeric molecules (such as nucleic acid) is described. The motion of tethered beads in flow is recognizable with a low-power optical microscope and distinguishable from that of beads that stick non-specifically to the flow cell surface. Analyte molecules are captured efficiently by capture beads and labeled efficiently by labeled detection antibody. The assay disclosed herein is sensitive enough for the detection of single molecules and/or the detection of very low concentrations of analyte in a sample.
PCT/US2015/034815 2014-06-20 2015-06-09 Methods for detection of an analyte by movement of tethered microparticles WO2015195404A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462015122P 2014-06-20 2014-06-20
US62/015,122 2014-06-20

Publications (2)

Publication Number Publication Date
WO2015195404A1 WO2015195404A1 (en) 2015-12-23
WO2015195404A8 true WO2015195404A8 (en) 2016-02-04

Family

ID=53434501

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2015/034815 WO2015195404A1 (en) 2014-06-20 2015-06-09 Methods for detection of an analyte by movement of tethered microparticles

Country Status (1)

Country Link
WO (1) WO2015195404A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3058372B1 (en) 2014-02-06 2023-07-05 Scanogen Inc. Detection units and methods for detecting a target analyte
US11703503B2 (en) 2016-12-28 2023-07-18 Technische Universiteit Eindhoven Biosensor based on capture molecules with different affinities
GB201807409D0 (en) * 2018-05-04 2018-06-20 Oxford Nanoimaging Ltd Assay
JP7203532B2 (en) * 2018-08-10 2023-01-13 シスメックス株式会社 Method for detecting test substance

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986002077A1 (en) 1984-10-02 1986-04-10 Meade Harry M Production of streptavidin-like polypeptides
WO1995007466A2 (en) 1993-09-08 1995-03-16 Baxter International Inc. Biotin-analog conjugated antibodies for positive cell selection and release
IL114149A0 (en) 1995-06-14 1995-10-31 Yeda Res & Dev Modified avidin and streptavidin molecules and use thereof
US6632929B1 (en) 1998-11-10 2003-10-14 Yeda Research And Development Co. Ltd Avidin derivatives and uses thereof
US6242610B1 (en) 1999-05-27 2001-06-05 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Derivatized biotin compounds and methods of use
FR2814463B1 (en) 2000-09-22 2002-11-15 Sanofi Synthelabo NOVEL POLYSACCHARIDES WITH ANTITHROMBOTIC ACTIVITY COMPRISING AT LEAST ONE COVALENT BINDING WITH BIOTIN OR A BIOTINE DERIVATIVE
US20050048012A1 (en) 2003-08-26 2005-03-03 Roland Jermann Use of biotin or a biotin derivative for skin lightening purposes and for the treatment of senile lentigines
US20070105162A1 (en) 2005-10-28 2007-05-10 Massachusetts Institute Of Technology Monovalent avidin and streptavidin compositions
US20080255004A1 (en) 2006-11-15 2008-10-16 Invitrogen Dynal As Methods of reversibly binding a biotin compound to a support
US20120220486A1 (en) * 2009-11-01 2012-08-30 Caerus Molecular Diagnostics Incorporated Methods and apparatus for binding assays
FR2964391B1 (en) * 2010-09-03 2014-04-11 Centre Nat Rech Scient BIOPUCES FOR ANALYSIS OF THE DYNAMICS OF NUCLEIC ACID MOLECULES

Also Published As

Publication number Publication date
WO2015195404A1 (en) 2015-12-23

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