WO2012153353A1 - Plasma expressor manual top and bottom - Google Patents
Plasma expressor manual top and bottom Download PDFInfo
- Publication number
- WO2012153353A1 WO2012153353A1 PCT/IN2012/000340 IN2012000340W WO2012153353A1 WO 2012153353 A1 WO2012153353 A1 WO 2012153353A1 IN 2012000340 W IN2012000340 W IN 2012000340W WO 2012153353 A1 WO2012153353 A1 WO 2012153353A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- expressor
- blood
- plasma
- pressure plate
- bag
- Prior art date
Links
- 210000004369 blood Anatomy 0.000 claims abstract description 18
- 239000008280 blood Substances 0.000 claims abstract description 18
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 8
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 6
- 239000010935 stainless steel Substances 0.000 claims abstract description 6
- 229910000831 Steel Inorganic materials 0.000 claims abstract description 5
- 239000010959 steel Substances 0.000 claims abstract description 5
- 239000012503 blood component Substances 0.000 claims abstract description 3
- 210000004027 cell Anatomy 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 2
- 239000000306 component Substances 0.000 abstract description 7
- 238000000926 separation method Methods 0.000 abstract description 2
- 238000012423 maintenance Methods 0.000 abstract 2
- 210000000265 leukocyte Anatomy 0.000 description 9
- 230000002265 prevention Effects 0.000 description 6
- 210000001772 blood platelet Anatomy 0.000 description 4
- 239000003634 thrombocyte concentrate Substances 0.000 description 4
- 206010060935 Alloimmunisation Diseases 0.000 description 3
- 208000003441 Transfusion reaction Diseases 0.000 description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 2
- 208000007587 Transfusion-Related Acute Lung Injury Diseases 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000000306 recurrent effect Effects 0.000 description 2
- 208000032467 Aplastic anaemia Diseases 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 206010011831 Cytomegalovirus infection Diseases 0.000 description 1
- 208000009329 Graft vs Host Disease Diseases 0.000 description 1
- 208000031886 HIV Infections Diseases 0.000 description 1
- 208000037357 HIV infectious disease Diseases 0.000 description 1
- 241000714260 Human T-lymphotropic virus 1 Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 208000024908 graft versus host disease Diseases 0.000 description 1
- 230000002949 hemolytic effect Effects 0.000 description 1
- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 1
- 229960003444 immunosuppressant agent Drugs 0.000 description 1
- 230000001861 immunosuppressant effect Effects 0.000 description 1
- 239000003018 immunosuppressive agent Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000007420 reactivation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/02—Blood transfusion apparatus
- A61M1/029—Separating blood components present in distinct layers in a container, not otherwise provided for
Definitions
- This invention related to improvement in device for preparation of Leuko Reduced Red Blood Cells.
- the aim of invention is to avail Leuko Reduced Red Blood Cells at all component blood bank to reduce the following adverse blood transfusion reactions.
- Leukocyte in blood components can cause:
- NFTR Non- hemolytic febrile transfusion reaction
- HLA Human-Leukocyte antigen
- CMV Epstein - Barr Virus
- HTLV-1 human - T-cell lymphotropic type
- Leukocytes-reduced red blood cells are indicated in the following conditions:
- Buffy coat-depleted red cells 10 8 Red cells, leukocyte-reduced by filtration ⁇ 10 7
- Red cell concentrates have on an average 10 8 leukocytes (one 'log leukocytes reduction). Thus the incidences of FNHTR are significantly reduced in comparison to red cells in additive solution prepared without removing buffy coat.
- the yield of platelets in platelet concentrates prepared from buffy coat is on an average 6-9 x 10'° and leuko-reduction is about 90% which is significantly more than that prepared from PRP. Platelet concentrates have on average 10 7 leukocytes.
- Leukocytes arc removed before storage, so less amount of cytokines are formed, and the number of degenerated white cells is less in red cells and platelet concentrate. Incidence of FNHTRs is further reduced by their transfusion.
- Expressure body It is made of thick steel plate. It contains the spring assembly with a pressure plate attached in front and handle attached on lateral wall. (Fig. 1)
- Fig.l shows plasma expressor top and bottom whole body.
- Fig.2 shows plasma expressor bottom view of the base.
- Fig.3 shows plasma expressor side view without cover for showing inner components.
- Fig.4 shows mostly used quadruple blood bag for preparation leuko reduced red blood cells and platelet concentration from buffy.
- Pressure Plater- Pressure plate is a thick, transparent acrylic flat plate which applies uniform pressure on the blood bag by means of a spring loaded mechanism.
- the pressure plate has a lock on one side by which the plate can be opened, which allows the blood bag to be placed between pressure plate and expressor body.
- Hook is used to hold the handle.
- the acrylic plate slide back allowing easy placement and removal of the centrifuged bag between the acrylic pressing plate and the expressor body.
- Stainless Steel Spring- It is situated in the bottom of the equipment.
- the spring is attached to a stainless steel rod which itself is connected to the pressure plate allowing the movement of the plate to be Break the 'break off valve' on the top of the blood bag for plasma satellite bag and on the bottom of the blood bag for red- ⁇ ells SAGM bag, this expresses the plasma and red cells into their respective bags.
Landscapes
- Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Biomedical Technology (AREA)
- Vascular Medicine (AREA)
- Engineering & Computer Science (AREA)
- Anesthesiology (AREA)
- Pathology (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- External Artificial Organs (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Plasma expressor manual top and bottom is an equipment used for separating plasma and buffy coat removed red blood cells. It is useful for all types of blood component bag. It is a mechanical device, which exerts uniform pressure on the blood bag during the separation of components. The plasma expressor includes a pressure plate (1), a handle hook (3), a rubber sheet (4), stainless steel springs (5), a steel rod (6), a expressor body (7) and a hanger (8). The current automated expressor is costly and requires maintenance. As no electrical components are used in this device, the manual top and bottom expressor is cheaper and requires no maintenance.
Description
1. Title
PLASMA EXPRESSOR MANUAL TOP AND BOTTOM
2. Technical field of invention
This invention related to improvement in device for preparation of Leuko Reduced Red Blood Cells.
3. Background and the 'prior art'
Many blood bank with component separation do not available Leuko Reduced Red Blood Cells, because previously automated plasma expressor top and bottom is costly. Causes many adverse blood transfusion reactions to patients.
4. Object of the invention
The aim of invention is to avail Leuko Reduced Red Blood Cells at all component blood bank to reduce the following adverse blood transfusion reactions.
Leukocyte in blood components can cause:
Non- hemolytic febrile transfusion reaction (NHFTR)
Human-Leukocyte antigen (HLA) alloimmunization
Transmission of leukotropic viruses (Cytomegalovirus)
(CMV), Epstein - Barr Virus (EBV) and- human - T-cell lymphotropic type (HTLV-1)
Transfusion related Graft versus host disease
Transfusion related acute lung injury (TRALI)
Transfusion related immunosuppressant
5. Statement of invention
Leukocytes-reduced red blood cells are indicated in the following conditions:
Multitransfused patients like thalassaemic
Leukemia
Aplastic anemia
Immunosupressed patients
Immunodeficient patients
Multiparous women
Prevention of recurrent FNHTRs
Prevention or delay of primary alloimmunization to HLA antigen Prevention of CMV transmission in at risk individuals.
Indications under investigation:
Prevention of platelet refractoriness due to alloimmunization
Prevention of recurrent FNHTRs to platelets
Prevention of reactivation of latent CMV or HIV infection
Approximate Residual . Leukocytes in Cellular Blood
Components.
Fresh whole blood 109
Red blood cells concentrate 108-109
Buffy coat-depleted red cells 108
Red cells, leukocyte-reduced by filtration <107
Washed red cells concentrate 107
Deglycerolized red 106-107
Platelet concentrate ≤107
6. A summary of invention
Conclusion
Red cell concentrates have on an average 108 leukocytes (one 'log leukocytes reduction). Thus the incidences of FNHTR are significantly reduced in comparison to red cells in additive solution prepared without removing buffy coat.
The yield of platelets in platelet concentrates prepared from buffy coat is on an average 6-9 x 10'° and leuko-reduction is about 90% which is significantly more than that prepared from PRP. Platelet concentrates have on average 107 leukocytes.
Leukocytes arc removed before storage, so less amount of cytokines are formed, and the number of degenerated white cells is less in red cells and platelet concentrate. Incidence of FNHTRs is further reduced by their transfusion.
Initial hard spin causes more yield of plasma leaving very little amount of plasma in red cells, this reduces the incidence of allergic and anaphylactic reactions after red cells transfusion. Besides, the yield of FFP is more and there by better yield of cryoprecipitate
controlled by handle itselt. It is adjustable and regulates the pressure of pressure plate. (Fig.2)
(6) Steel rod- It is attached to a mechanism of spring, pressure plate and handle.
(Fig-3)
(7) Expressure body- It is made of thick steel plate. It contains the spring assembly with a pressure plate attached in front and handle attached on lateral wall. (Fig. 1)
(8) Hanger- It is fitted at the top of expressor body in horizontal position. It's function is to hold the centrifuged blood bag. (Fig. 1) Operating Procedure: The plasma expressor manual top and bottom has a transparent pressure plate driven by an adjustable stainless steel spring mechanism which has a handle attached to it. Hook the handle and unlock the pressure plate. Place the centrifuged blood bag on the hanger provided, lock the pressure plate and unhook the
7. A brief description of the accompanying drawing
Fig.l shows plasma expressor top and bottom whole body.
Fig.2 shows plasma expressor bottom view of the base.
Fig.3 shows plasma expressor side view without cover for showing inner components.
Fig.4 shows mostly used quadruple blood bag for preparation leuko reduced red blood cells and platelet concentration from buffy.
8. Detailed description of the invention with reference to drawing/ example
Equipment Sketch and controls
(1) Pressure Plater- Pressure plate is a thick, transparent acrylic flat plate which applies uniform pressure on the blood bag by means of a spring loaded mechanism. The pressure plate has a lock on one side by which the plate can be opened, which allows the blood bag to be placed between pressure plate and expressor body.
There is a gap both above and below the plate allowing top and bottom satellite bag to be attached. This differs from the conventional manual plasma expressor in which there in a gap only on the top while the lower end of the plate is attached to the body. (Fig.l)
(2) Handler- Handle is a stainless steel rod attached to the lateral side of expressor body which is provided with a ball at the end for ease of handling. It is movable in small angles to be hooked and unhooked. Pressing the handle slides the pressure plate away from the expressor body in a way which allows the blood bag to be placed in the gap. (Fig.l)
(3) Hook:-
Hook is used to hold the handle. When the handle is placed inside the hook,, the acrylic plate slide back allowing easy placement and removal of the centrifuged bag between the acrylic pressing plate and the expressor body. (Fig.l)
(4) Rubber Sheet- It is "fixed between the expressor body and pressure plate. The function is to minimize the gap and help in better uniform extraction of the components. (Fig.l)
(5) Stainless Steel Spring- It is situated in the bottom of the equipment. The spring is attached to a stainless steel rod which itself is connected to the pressure plate allowing the movement of the plate to be
Break the 'break off valve' on the top of the blood bag for plasma satellite bag and on the bottom of the blood bag for red- ^ells SAGM bag, this expresses the plasma and red cells into their respective bags.
Observe the plasma and red cells flowing out of the primary bag to satellite bag. When the 'plasma and the buffy coat' portion reach the tube, clamp, the tube. Same procedure to be done for red cells SAGM bag. Release the pressure on the primary bag by putting the handle in the hooked position.
Use a tube sealer to seal off the tube and detach the plasma bag and red cells SAGM bag from primary bag. It leaves leukocytes rich buffy coat and a small amount of red cells in the primary bag.
Claims
1. I/We claim, an equipment plasma expressor manual top and bottom which is used for Leuko reduced Red Blood Cells (Blood Component) preparation. Which have part expressor plate, hadle hook, rubber sheet, stainless steel spring, steel rod,expressor body and hanger.
2. An apparatus as claimed in claim 1 where is leukoreduced red cells is prepared by a manual method. The spring is used as a pressure for pressure plate.
3. An apparatus as claimed in claim 1 the mechanism of spring, steel rod and the pressure plate mechanism operated by handle.
4. An apparatus as we claimed in claim 3 the pressure plate is opened one side for hanging the centrifuged blood bag in hanger. The pressure plate is working both top and bottom blood bags as well as top and top blood bags are operated on this manual expressor.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN1368/DEL/2011 | 2011-05-10 | ||
| IN1368DE2011 | 2011-05-10 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2012153353A1 true WO2012153353A1 (en) | 2012-11-15 |
| WO2012153353A8 WO2012153353A8 (en) | 2013-05-30 |
Family
ID=47138861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/IN2012/000340 WO2012153353A1 (en) | 2011-05-10 | 2012-05-09 | Plasma expressor manual top and bottom |
Country Status (1)
| Country | Link |
|---|---|
| WO (1) | WO2012153353A1 (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4284209A (en) * | 1979-06-21 | 1981-08-18 | Barbour Jr Robert E | Device and method for collecting blood plasma |
| EP0557900A2 (en) * | 1992-02-25 | 1993-09-01 | Heinz Krüssel | Blood components separating device |
| CN2210703Y (en) * | 1994-06-21 | 1995-10-25 | 徐州市红十字中心血站 | Blood plasma-separating clip |
| JP2000070335A (en) * | 1998-09-02 | 2000-03-07 | Kawasumi Lab Inc | Liquid storage container pressing aid |
| JP3394785B2 (en) * | 1991-03-12 | 2003-04-07 | テルモ株式会社 | Liquid separation device |
| CN201132383Y (en) * | 2007-10-15 | 2008-10-15 | 武汉金德涞生物科技有限公司 | Air bubbles extruder for blood plasma bag |
-
2012
- 2012-05-09 WO PCT/IN2012/000340 patent/WO2012153353A1/en active Application Filing
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4284209A (en) * | 1979-06-21 | 1981-08-18 | Barbour Jr Robert E | Device and method for collecting blood plasma |
| JP3394785B2 (en) * | 1991-03-12 | 2003-04-07 | テルモ株式会社 | Liquid separation device |
| EP0557900A2 (en) * | 1992-02-25 | 1993-09-01 | Heinz Krüssel | Blood components separating device |
| CN2210703Y (en) * | 1994-06-21 | 1995-10-25 | 徐州市红十字中心血站 | Blood plasma-separating clip |
| JP2000070335A (en) * | 1998-09-02 | 2000-03-07 | Kawasumi Lab Inc | Liquid storage container pressing aid |
| CN201132383Y (en) * | 2007-10-15 | 2008-10-15 | 武汉金德涞生物科技有限公司 | Air bubbles extruder for blood plasma bag |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012153353A8 (en) | 2013-05-30 |
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