WO2012137413A1 - 流体取扱装置及び流体取扱システム - Google Patents

流体取扱装置及び流体取扱システム Download PDF

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Publication number
WO2012137413A1
WO2012137413A1 PCT/JP2012/001690 JP2012001690W WO2012137413A1 WO 2012137413 A1 WO2012137413 A1 WO 2012137413A1 JP 2012001690 W JP2012001690 W JP 2012001690W WO 2012137413 A1 WO2012137413 A1 WO 2012137413A1
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Prior art keywords
region
fluid handling
film
regions
chip
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Ceased
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PCT/JP2012/001690
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English (en)
French (fr)
Inventor
中尾　智貴
小野　航一
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Enplas Corp
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Enplas Corp
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Priority to US14/110,086 priority Critical patent/US9120098B2/en
Publication of WO2012137413A1 publication Critical patent/WO2012137413A1/ja
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
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Definitions

  • the present invention relates to a fluid handling device used for analysis and processing of a liquid sample and a fluid handling system including the fluid handling device.
  • microanalysis systems have been used in the scientific field or the medical field such as biochemistry and analytical chemistry in order to perform inspection and analysis of trace amounts of substances such as proteins and nucleic acids (for example, DNA) with high accuracy and high speed.
  • the flow path formed in the micro flow path chip is filled with electrophoresis solution (buffer solution), a sample is injected from an injection port connected to the flow path, a voltage is applied to both ends of the flow path, There is a system for performing analysis by electrophoresis.
  • electrophoresis solution buffer solution
  • a microchannel chip is manufactured by bonding a film (thin film) or a thin plate to a chip body in which a channel is formed. At both ends of the flow path, reservoirs into which liquid is injected are formed, and electrodes are formed in the reservoirs.
  • an electrode forming method a method of printing an electrode pattern on a film or thin plate with carbon ink is known (see Patent Document 1). One end of the electrode pattern thus formed is formed so as to be located in the reservoir, and the other end is formed to be located outside the reservoir. The electrode of the electrophoresis apparatus is brought into contact with the other end of the electrode pattern, and a voltage is applied to the liquid sample without touching the liquid sample injected into the reservoir.
  • the peripheral portion of the carbon ink of the film or thin plate may not adhere to the chip body (lamination failure), and liquid leakage may occur.
  • the conventional technology does not take measures against the leakage of the liquid, and the electrophoretic device (electrode) may be contaminated when the liquid leaks.
  • An object of the present invention is to provide a fluid handling device and a fluid handling system capable of preventing leakage of liquid from the fluid handling device such as a micro-channel chip to the outside and preventing contamination of the external environment. It is.
  • the fluid handling device of the present invention includes a substrate member in which a recess or a through-hole is formed, a thin film-like or thin plate-like lid member joined to one surface of the substrate member, and the lid member side of the lid member And a transfer function part for transmitting electricity or heat, which is formed in a layer so as to cover a part of the surface of the substrate member, and a portion corresponding to one end of the transfer function part of the substrate member has a first region And the opening on the lid member side of the recess or through hole forming the first region is closed by the lid member, and the transmission function of the substrate member A second region communicating with the outside is formed in a portion corresponding to the other end of the unit, and the transfer function unit electrically or thermally connects the first region and the second region.
  • a recess forming a third region is formed so as to span an edge of the transmission function portion, and the lid of the recess forming the third region
  • the member-side opening is closed by the lid member, and the third region communicates with the second region and is filled with an adhesive.
  • the fluid handling device system of the present invention employs a configuration including the fluid handling device.
  • the substrate is formed by the thickness of the layered transmission function part by forming the recess in the substrate member and filling the space (third region) formed by closing the recess with the thin film or the thin plate with the adhesive. It is possible to prevent leakage of liquid that has leaked through the gap between the member and the lid member, and to prevent contamination of the external environment.
  • FIGS. 1A to 1D are diagrams showing the shape of a microchannel chip according to an embodiment of the present invention.
  • 2A to 2D are views showing the shape of the chip body of the microchannel chip shown in FIGS. 1A to 1D.
  • 3A to 3C are diagrams showing the shape of the film after the carbon ink printing of the microchannel chip shown in FIGS. 1A to 1D.
  • FIG. 1B is an enlarged sectional view taken along line EE shown in FIG. 1A.
  • 5A to 5C are diagrams showing the shape of the microchannel chip according to the embodiment of the present invention (variation 1).
  • 6A to 6C are diagrams showing the shape of the chip body of the microchannel chip shown in FIGS. 5A to 5C.
  • FIGS. 1A to 1D are diagrams showing the shape of a microchannel chip as a fluid handling apparatus according to the present embodiment.
  • 1A is a plan view
  • FIG. 1B is a front sectional view taken along line AA
  • FIG. 1C is a bottom view
  • FIG. 1D is a left sectional view taken along line BB.
  • 1A and 1B show both electrode rods 1a and 1b.
  • a microchannel chip 10 includes a transparent substantially rectangular flat plate chip body (substrate member) 12, a film (a thin film serving as a lid member) 14, and carbon ink (as a transmission function unit). Electrode) 16a, 16b.
  • the thickness of the chip body 12 is about 1 mm, the thickness of the film 14 is about 100 ⁇ m, and the thickness of the carbon inks 16 a and 16 b is about 10 ⁇ m.
  • the chip body 12 and the film 14 are formed of a resin material such as polyethylene terephthalate, polycarbonate, polymethyl methacrylate, vinyl chloride, polypropylene, polyether, polyethylene, and the like. Note that different materials may be used for the chip body 12 and the film 14.
  • Carbon inks 16 a and 16 b are printed on the film 14.
  • the film 14 is bonded to the chip body 12 by adhesion with an organic adhesive or thermocompression bonding.
  • FIGS. 2A to 2D are diagrams showing the shape of the chip body 12.
  • 2A is a plan view
  • FIG. 2B is a front sectional view taken along the line CC
  • FIG. 2C is a bottom view
  • FIG. 2D is a left side sectional view taken along the line DD.
  • the chip body 12 is provided with elongated fine grooves 22 on a lower surface 21 which is a surface facing the film 14.
  • the fine groove 22 has a substantially rectangular cross section having a side length (width and depth) of about several tens of ⁇ m. In the state where the chip body 12 and the film 14 are joined, the opening portion of the fine groove 22 is closed by the film 14, thereby forming the flow path 22 ′.
  • through holes 23a and 23b having a substantially circular cross section are formed.
  • the diameters of the through holes 23a and 23b are several hundred ⁇ m to several mm.
  • the opening portions of the through holes 23a and 23b are closed by the film 14, thereby having a bottomed function having a function as an injection port or a discharge port for the electrophoresis solution or the sample.
  • First regions 23a ′ and 23b ′ are formed.
  • through holes 24a and 24b having a substantially circular cross section are formed.
  • the diameters of the through holes 24a and 24b are several hundred ⁇ m to several mm.
  • the opening portions of the through holes 24a and 24b are closed by the film 14, so that the bottomed second having a function as an insertion port for the electrode rods 1a and 1b. Regions 24a 'and 24b' are formed.
  • a recess 25a is formed at a position on the carbon ink 16a and connected to the through hole 24a
  • a recess 25b is formed at a position on the carbon ink 16b and connected to the through hole 24b.
  • the third regions 25a 'and 25b' are filled with conductive adhesives 26a and 26b (see FIGS. 1 and 4).
  • the unbonded portion of the chip body 12 and the film 14 generated by the thickness of the carbon inks 16a and 16b becomes an unexpected flow path, and the liquid leaking from the first regions 23a ′ and 23b ′ is the second region 24a ′. , 24b ′ can be prevented.
  • 3A to 3C are diagrams showing the shape of the film 14 after the carbon inks 16a and 16b are printed.
  • 3A is a plan view
  • FIG. 3B is a front view
  • FIG. 3C is a left side view.
  • the film 14 on which the carbon inks 16a and 16b are printed has a transparent organic adhesive on the lower surface 21 of the chip body 12 so as to cover at least the fine grooves 22, the through holes 23a, 23b, 24a and 24b, and the recesses 25a and 25b. Bonded by bonding or thermocompression bonding.
  • both ends of the carbon ink 16a are located in the first region 23a ′ and the second region 24a ′, and both ends of the carbon ink 16b are in the first region 23b ′.
  • the carbon inks 16a and 16b are electrically conductive and have a function as an electrode.
  • Electrophoresis using microchannel chip First, in the microchannel chip 10, the electrophoresis solution is injected into the first region (injection port) 23a ′ to fill the channel 22 ′. Next, a sample for analysis is injected. Further, the electrode rods 1a and 1b are inserted into the second regions 24a ′ and 24b ′ and brought into contact with the conductive adhesives 26a and 26b.
  • the sample is separated by the difference in moving speed for each molecular weight.
  • the tester can obtain the result of electrophoresis by detecting the fluorescence intensity.
  • FIG. 4 is an enlarged cross-sectional view taken along the line EE of FIG. 1A.
  • the peripheral portion of the carbon ink 16b (16a) of the film 14 is not adhered to the chip body 12 (lamination failure), and the carbon ink There is a possibility that gaps 31a and 31b are formed between the chip body 12 and the film 14 at the edge of 16a (16b).
  • the gaps 31a and 31b become unexpected flow paths that connect to the first region 23b '(23a'). Therefore, the liquid (electrophoretic solution, sample) injected into the flow path 22 ′ of the micro flow path chip 10 leaks from the first region 23 b ′ (23 a ′) through the gaps 31 a and 31 b due to capillary action.
  • the third region 25b '(25a') is formed in the microchannel chip 10 according to the present embodiment.
  • the width W1 of the third region 25b '(25a') is formed to be longer than the width W2 of the carbon ink 16b (16a), and the third region 25b '(25a') is connected to the gaps 31a and 31b.
  • the opening of the gaps 31a and 31b to the third region 25b '(25a') is closed.
  • the liquid does not travel from the first region 23b '(23a') to the second region 24a '(24b') through the gaps 31a and 31b.
  • FIGS. 5A to 5C are diagrams showing shapes of variation 1 of the microchannel chip according to the present embodiment.
  • the variation 1 microchannel chip 10-1 is used to heat a sample with a heater.
  • 5A is a plan view
  • FIG. 5B is a front sectional view taken along line FF
  • FIG. 5C is a bottom view.
  • 5A and 5B show both the electric heater 1a-1.
  • 5A to 5C parts common to those in FIGS. 1A to 1D are denoted by the same reference numerals, and detailed description thereof is omitted.
  • FIGS. 5A to 5C are diagrams showing the shape of the chip body of the microchannel chip shown in FIGS. 5A to 5C.
  • 6A is a plan view
  • FIG. 6B is a front sectional view taken along line GG
  • FIG. 6C is a bottom view.
  • 6A to 6C parts common to those in FIGS. 2A to 2D are denoted by the same reference numerals, and detailed description thereof is omitted.
  • Variation 1 is a case where there is only one metal film 16a-1 having a high heat transfer property as the transfer function part. 5A to 5C, the microchannel chip 10-1 is different from the chip body 12 and the film 14 shown in FIGS. 1A to D in the shape of the chip body 12-1 and the film 14-1.
  • a through hole 23a, a through hole 24a, and a through hole 27 are formed in the chip body 12-1 one by one. Further, one recess 25a is formed on the lower surface 21-1 of the chip body 12-1. Note that no fine groove is formed in the chip body 12-1.
  • the through hole 27 serves as an adhesive inlet 27 ′ that communicates with the third region 25 a ′ in a state where the chip body 12-1 and the film 14-1 are joined.
  • the adhesive 26-1 that fills the third region 25a ' is injected from the injection port 27'.
  • the adhesive 26-1 is introduced into the third region 25a 'by capillary action, and stops at the opening of the third region 25a' to the second region 24a 'by capillary action. Accordingly, since the metal film 16a-1 in the second region 24a 'is not covered with the adhesive 26-1, the electric heater 1a-1 can be brought into direct contact with the metal film 16a-1.
  • the flow of the adhesive 26-1 flowing in the flow path-shaped third region 25a ′ stops when it reaches the opening of the second region 24a ′, which is a large space, and the second region 24a ′.
  • the inner transfer function part (metal film) 16a-1 is not covered.
  • the adhesive 26-1 only needs to prevent communication between the second region 24a ′ and the third region 25a ′, and is not limited to one having a transmission function. Can do.
  • the present invention is not limited to this, and other conductive members and heat transfer members are used. Even if a member is used, the same effect can be obtained.
  • the present invention is not limited to this, and a case where a thin plate is joined to the chip body as shown in FIG. A similar effect can be obtained.
  • the present invention provides a space between a space (first region) into which a liquid is introduced and a space (second region) electrically or thermally connected to the space (second region).
  • a space (third region) communicating with a gap (unexpected flow path) that may occur at the edge of the transmission function portion is formed, and the third region is filled with an adhesive, thereby forming a space in the first region.
  • the liquid to be introduced is prevented from leaking into the space of the second region.
  • the concave shape of the substrate member for forming the first region and the second region is not limited to the shape shown in the above embodiment.
  • the first region may be a space in the middle of the flow path.
  • the fluid handling apparatus and fluid handling system according to the present invention can be used in an apparatus that performs high-speed inspection analysis of a very small amount of substances in the scientific field or the medical field such as biochemistry and analytical chemistry.
  • Micro-channel chip 12 12-1 Chip body 14, 14-1 Film 16a, 16b Carbon ink 16a-1 Metal film 22 Fine groove 22 'Channel 23a, 23b, 24a, 24b, 27 Through hole 23a', 23b ', 26' First region 24a ', 24b' Second region 25a, 25b Recess 25a ', 25b' Third region 26a ', 26b', 26-1 Adhesive 27 'Inlet

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Abstract

　本発明は、ラミネート不良に起因する液体の漏れを防止できるマイクロ流路チップに関する。マイクロ流路チップ（１０）のチップ本体（１２）の下面（２１）にフィルム（１４）を接合することにより、有底の第１領域（２３ａ'、２３ｂ'）、第２領域（２４ａ'、２４ｂ'）、第３領域（２５ａ'、２５ｂ'）が形成される。第３領域（２５ａ'、２５ｂ'）は、第２領域（２４ａ'、２４ｂ'）に連通し、カーボンインク（１６ａ、１６ｂ）上に形成される。第３領域（２５ａ'、２５ｂ'）の幅は、カーボンインク（１６ａ、１６ｂ）の幅より長くなるように形成される。第３領域（２５ａ'、２５ｂ'）には、導電性の接着剤が充填される。

Description

流体取扱装置及び流体取扱システム
　本発明は、液体試料の分析や処理等に用いられる流体取扱装置及びこれを具備する流体取扱システムに関する。
　近年、生化学や分析化学等の科学分野あるいは医学分野において、タンパクや核酸（例えば、ＤＮＡ）などの微量な物質の検査分析を精度良く高速に行うために、マイクロ分析システムが使用されている。
　マイクロ分析システムの一例として、マイクロ流路チップに形成された流路を泳動液（緩衝液）で満たし、流路に繋がる注入口から試料を注入し、流路の両端に電圧をかけ、試料を電気泳動させて分析を行うシステムがある。
　マイクロ流路チップは、流路が形成されたチップ本体にフィルム（薄膜）または薄板を接合することにより作製される。流路の両端には、液体が注入されるリザーバが形成され、そのリザーバ内には電極が形成される。電極形成方法の例として、カーボンインクにてフィルムまたは薄板上に電極パターンを印刷する方法が知られている（特許文献１参照）。そのように形成される電極パターンの一端は、リザーバ内に位置するように形成され、他端はリザーバ外に位置するように形成されている。そして、電極パターンの他端に電気泳動装置の電極を接触させ、リザーバ内に注入される液体試料に触れることなく液体試料に電圧がかけられるように構成されている。
米国特許第６９３９４５１号明細書
　上記の電極形成方法では、カーボンインクの厚みにより、フィルムまたは薄板のカーボンインクの周辺部分がチップ本体に未接着となり（ラミネート不良）、液体の漏れが発生するおそれがある。
　しかしながら、従来技術では、この液体の漏れについて対策が採られておらず、液体が漏れた場合に、電気泳動装置（電極）が汚染するおそれがある。
　本発明の目的は、マイクロ流路チップ等の流体取扱装置から外部への液体の漏れを防止することができ、外部環境の汚染を防止することができる流体取扱装置及び流体取扱システムを提供することである。
　本発明の流体取扱装置は、凹みまたは貫通孔が形成された基板部材と、前記基板部材の一方の面に接合された、薄膜状または薄板状の蓋部材と、前記蓋部材の前記基板部材側の面の一部を覆うように層状に形成された、電気または熱を伝達する伝達機能部と、を有し、前記基板部材の前記伝達機能部の一端に対応する部分には、第１領域を形成する凹みまたは貫通孔が形成されており、前記第１領域を形成する凹みまたは貫通孔の前記蓋部材側の開口部は、前記蓋部材により閉塞されており、前記基板部材の前記伝達機能部の他端に対応する部分には、外部に連通する第２領域が形成されており、前記伝達機能部は、前記第１領域と前記第２領域との間を電気的または熱的に接続しており、前記基板部材の前記伝達機能部の前記一端および前記他端との間の部分に対応する部分には、第３領域を形成する凹みが前記伝達機能部の縁部に架かるように形成されており、前記第３領域を形成する凹みの前記蓋部材側の開口部は、前記蓋部材により閉塞されており、前記第３領域は、前記第２領域と連通しており、かつ接着剤で充填されている、構成を採る。
　本発明の流体取扱装置システムは、上記流体取扱装置を備える構成を採る。
　本発明によれば、基板部材に凹みを形成し、この凹みが薄膜または薄板によって閉塞されてできる空間（第３領域）を接着剤で充填することにより、層状の伝達機能部の厚みによって生じる基板部材と蓋部材との隙間を伝わって漏れてきた液体の漏れを防止することができ、外部環境の汚染を防止することができる。
図１Ａ～Ｄは、本発明の一実施の形態に係るマイクロ流路チップの形状を示す図である。 図２Ａ～Ｄは、図１Ａ～Ｄに示されるマイクロ流路チップのチップ本体の形状を示す図である。 図３Ａ～Ｃは、図１Ａ～Ｄに示されるマイクロ流路チップのカーボンインク印刷後のフィルムの形状を示す図である。 図１Ａに示されるＥ－Ｅ線拡大断面図である。 図５Ａ～Ｃは、本発明の一実施の形態に係るマイクロ流路チップの形状を示す図である（バリエーション１）。 図６Ａ～Ｃは、図５Ａ～Ｃに示されるマイクロ流路チップのチップ本体の形状を示す図である。
　以下、本発明の実施の形態について、図面を参照して詳細に説明する。
　〔マイクロ流路チップの構成〕
　図１Ａ～Ｄは、本実施の形態に係る流体取扱装置としてのマイクロ流路チップの形状を示す図である。図１Ａは平面図、図１ＢはＡ－Ａ線正面断面図、図１Ｃは底面図、図１ＤはＢ－Ｂ線左側面断面図である。なお、図１Ａおよび図１Ｂには、電極棒１ａ、１ｂをともに示す。
　図１Ａ～Ｄに示すように、マイクロ流路チップ１０は、透明な略矩形の平板のチップ本体（基板部材）１２とフィルム（蓋部材となる薄膜）１４と、カーボンインク（伝達機能部としての電極）１６ａ、１６ｂと、から構成される。
　チップ本体１２の厚さは１ｍｍ程度であり、フィルム１４の厚さは１００μｍ程度であり、カーボンインク１６ａ、１６ｂの厚さは１０μｍ程度である。
　チップ本体１２およびフィルム１４は、例えばポリエチレンテレフタレート、ポリカーボネート、ポリメタクリル酸メチル、塩化ビニール、ポリプロピレン、ポリエーテル、ポリエチレンなどの樹脂材料によって形成されている。なお、チップ本体１２とフィルム１４とで、互いに異なる材料を用いても良い。
　カーボンインク１６ａ、１６ｂは、フィルム１４に印刷される。フィルム１４は、有機接着剤による接着あるいは熱圧着等によりチップ本体１２に接合される。
　図２Ａ～Ｄは、チップ本体１２の形状を示す図である。図２Ａは平面図、図２ＢはＣ－Ｃ線正面断面図、図２Ｃは底面図、図２ＤはＤ－Ｄ線左側面断面図である。
　チップ本体１２には、フィルム１４に対向する面である下面２１に、細長い微細溝２２が形成される。微細溝２２は、一辺の長さ（幅および深さ）が数十μｍ程度の略矩形の断面を有する。チップ本体１２とフィルム１４とが接合された状態において、微細溝２２の開口部分がフィルム１４によって閉塞されることにより流路２２’が形成される。
　チップ本体１２の微細溝２２のそれぞれの両端には、外部に開口する、断面が略円形の貫通孔２３ａ、２３ｂが形成される。貫通孔２３ａ、２３ｂの直径は、数１００μｍ～数ｍｍである。チップ本体１２とフィルム１４とが接合された状態において、貫通孔２３ａ、２３ｂの開口部分がフィルム１４によって閉塞されることにより、泳動液、試料の注入口又は排出口としての機能を有する有底の第１領域２３ａ’、２３ｂ’が形成される。
　チップ本体１２には、断面が略円形の貫通孔２４ａ、２４ｂが形成される。貫通孔２４ａ、２４ｂの直径は、数１００μｍ～数ｍｍである。チップ本体１２とフィルム１４とが接合された状態において、貫通孔２４ａ、２４ｂの開口部分がフィルム１４によって閉塞されることにより、電極棒１ａ、１ｂの挿入口としての機能を有する有底の第２領域２４ａ’、２４ｂ’が形成される。
　チップ本体１２の下面２１には、カーボンインク１６ａ上の位置に貫通孔２４ａに連接して凹み２５ａが形成され、カーボンインク１６ｂ上の位置に貫通孔２４ｂに連接して凹み２５ｂが形成される。そして、チップ本体１２とフィルム１４とが接合された状態において、凹み２５ａ、２５ｂの開口部がフィルム１４によって閉塞されることにより第３領域２５ａ’、２５ｂ’が形成される。第３領域２５ａ’、２５ｂ’は、カーボンインク１６ａ、１６ｂ上に位置する。第３領域２５ａ’、２５ｂ’の幅は、カーボンインク１６ａ、１６ｂの幅より長くなるように形成される（図４参照）。第３領域２５ａ’、２５ｂ’には、導電性の接着剤２６ａ、２６ｂ（図１、図４参照）が充填される。これにより、カーボンインク１６ａ、１６ｂの厚みによって生じるチップ本体１２とフィルム１４の未接着部分が予期しない流路となって、第１領域２３ａ’、２３ｂ’から漏れてきた液体が第２領域２４ａ’、２４ｂ’に到達することを防止することができる。
　図３Ａ～Ｃは、カーボンインク１６ａ、１６ｂ印刷後のフィルム１４の形状を示す図である。図３Ａは平面図、図３Ｂは正面図、図３Ｃは左側面図である。
　カーボンインク１６ａ、１６ｂが印刷されたフィルム１４は、少なくとも微細溝２２、貫通孔２３ａ、２３ｂ、２４ａ、２４ｂ、凹み２５ａ、２５ｂを覆うように、チップ本体１２の下面２１に、透明な有機接着剤による接着あるいは熱圧着等により接合される。
　フィルム１４をチップ本体１２に接合した際には、カーボンインク１６ａの両端が第１領域２３ａ’の中および第２領域２４ａ’の中に位置し、カーボンインク１６ｂの両端が第１領域２３ｂ’の中および第２領域２４ｂ’の中に位置する。カーボンインク１６ａ、１６ｂは、導電性があり、電極としての機能を有する。
　〔マイクロ流路チップによる電気泳動〕
　まず、マイクロ流路チップ１０において、第１領域（注入口）２３ａ’に泳動液を注入し、流路２２’を充填する。次に分析用の試料を注入する。また、電極棒１ａ、１ｂを、第２領域２４ａ’、２４ｂ’に挿入して導電性接着剤２６ａ、２６ｂに接触させる。
　そして、電極棒１ａ、１ｂに通電することにより、流路２２’の両端に電圧をかける。これにより、試料は、第１領域（排出口）２３ｂ’に向かって流路２２’内を移動する。
　流路２２’内において、試料は、分子量毎の移動速度の違いによって分離される。試験者は、蛍光強度を検出することにより、電気泳動の結果を得ることができる。
　〔本実施の形態の効果〕
　図４は、図１ＡのＥ－Ｅ線拡大断面図である。図４に示すように、カーボンインクの１６ｂ（１６ａ）の厚みに起因して、フィルム１４のカーボンインクの１６ｂ（１６ａ）の周辺部分がチップ本体１２に未接着となり（ラミネート不良）、カーボンインクの１６ａ（１６ｂ）の縁部において、チップ本体１２とフィルム１４との間に隙間３１ａ、３１ｂが生じる虞がある。
　隙間３１ａ、３１ｂは、第１領域２３ｂ’（２３ａ’）に繋がる予期しない流路となる。したがって、マイクロ流路チップ１０の流路２２’に注入した液体（泳動液、試料）が、毛細管現象により、第１領域２３ｂ’（２３ａ’）から隙間３１ａ、３１ｂを伝わって漏れてくる。
　本実施の形態に係るマイクロ流路チップ１０には、第３領域２５ｂ’（２５ａ’）が形成される。第３領域２５ｂ’（２５ａ’）の幅Ｗ１は、カーボンインク１６ｂ（１６ａ）の幅Ｗ２より長くなるように形成され、第３領域２５ｂ’（２５ａ’）は隙間３１ａ、３１ｂと繋がっている。この第３領域２５ｂ’（２５ａ’）に導電性の接着剤２６ｂ（２６ａ）を充填することにより、隙間３１ａ、３１ｂの第３領域２５ｂ’（２５ａ’）への開口部が塞がる。
　したがって、第１領域２３ｂ’（２３ａ’）から、液体が、隙間３１ａ、３１ｂを伝わって第２領域２４ａ’（２４ｂ’）に行くことはない。
　この結果、本実施の形態によれば、液体の外部への漏れを防止することができ、電極や外部環境の汚染を防止することができる。
　［バリエーション］
　以下、本実施の形態に係るマイクロ流路チップのバリエーションについて説明する。
　［バリエーション１］
　図５Ａ～Ｃは、本実施の形態に係るマイクロ流路チップのバリエーション１の形状を示す図である。バリエーション１のマイクロ流路チップ１０－１は、試料をヒータで加熱するために用いられる。図５Ａは平面図、図５ＢはＦ－Ｆ線正面断面図、図５Ｃは底面図である。なお、図５Ａおよび図５Ｂには、電熱ヒータ１ａ－１をともに示す。なお、図５Ａ～Ｃにおいて、図１Ａ～Ｄと共通する部分には、同一の符号を付して詳しい説明を省略する。
　図６Ａ～Ｃは、図５Ａ～Ｃに示されるマイクロ流路チップのチップ本体の形状を示す図である。図６Ａは平面図、図６ＢはＧ－Ｇ線正面断面図、図６Ｃは底面図である。なお、図６Ａ～Ｃにおいて、図２Ａ～Ｄと共通する部分には、同一の符号を付して詳しい説明を省略する。
　バリエーション１は、伝達機能部としての伝熱性に富む金属膜１６ａ－１が一つのみの場合である。図５Ａ～Ｃにおいて、マイクロ流路チップ１０－１は、チップ本体１２－１、フィルム１４－１の形状が図１Ａ～Ｄに示されるチップ本体１２、フィルム１４と異なる。
　また、図６Ａ～Ｃにおいて、チップ本体１２－１には、貫通孔２３ａ、貫通孔２４ａ、貫通孔２７が一つずつ形成される。また、チップ本体１２－１の下面２１－１には、凹み２５ａが一つ形成される。なお、チップ本体１２－１には、微細溝は形成されていない。
　貫通孔２７は、チップ本体１２－１とフィルム１４－１とが接合された状態において、第３領域２５ａ’と連通する接着剤の注入口２７’となる。バリエーション１のマイクロ流路チップ１０－１は、第３領域２５ａ’を充填させる接着剤２６－１を注入口２７’より注入する。接着材２６－１は毛細管現象によって第３領域２５ａ’へ導入され、毛細管現象によって第３領域２５ａ’の第２領域２４ａ’への開口部で止まる。これより、第２領域２４ａ’内の金属膜１６ａ－１は接着剤２６－１で覆われないため、電熱ヒータ１ａ－１を金属膜１６ａ－１に直接、接触させることができる。
　このように、流路状の第３領域２５ａ’内を流動する接着剤２６－１は、広い空間である第２領域２４ａ’の開口部に達したところでその流れは止まり、第２領域２４ａ’内の伝達機能部(金属膜)１６ａ－１を覆い隠すことはない。接着剤２６－１は、第２領域２４ａ’と第３領域２５ａ’との間が連通するのを妨げるものであればよく、伝達機能を有するものに限定されないため、選択の自由度を広げることができる。
　バリエーション１によっても、第１領域２３ａ’から漏れてきた液体が、第３領域２５ａ’に止められ、第２領域２４ａ’に到達するのを防ぐことができる。この結果、液体の漏れを防止することができ、外部環境の汚染を防止することができ、電熱ヒータ１ａ－１によって安全に第１領域２３ａ’内に注入された試料を加熱することができる。
　なお、上記実施の形態としては、導電部材としてカーボンインク１６ａ、１６ｂ、伝熱部材として金属膜１６ａ－１を用いる場合について説明したが、本発明はこれに限られず、他の導電部材や伝熱部材を用いても同様の効果を得ることができる。
　また、上記実施の形態としては、フィルム１４をチップ本体１２に接合する場合について説明したが、本発明はこれに限られず、特許文献１の図１のように、薄板をチップ本体に接合する場合にも同様の効果を得ることができる。
　本発明は、上記実施の形態に示す通り、液体が導入される空間（第１領域）と、これに電気的または熱的に伝達機能部によって接続されている空間（第２領域）との間に、伝達機能部の縁部に生じ得る隙間（予期しない流路）に連通する空間（第３領域）を形成し、この第３領域を接着剤で充填させることによって、第１領域の空間に導入される液体が第２領域の空間に漏れ出るのを防ぐものである。この効果が得られるものであれば、第１領域および第２領域を形成するための基板部材の凹み形状は上記実施の形態において示した形状に限られない。第１領域は、流路の途中の空間であってもよい。
　本出願は、２０１１年４月４日出願の特願２０１１－０８２８２０に基づく優先権を主張する。当該出願明細書および図面に記載された内容は、すべて本願明細書に援用される。
　本発明に係る流体取扱装置及び流体取扱システムは、生化学や分析化学等の科学分野あるいは医学分野において、微量な物質の検査分析を精度良く高速に行う装置に使用することができる。
　１０　マイクロ流路チップ
　１２、１２－１　チップ本体
　１４、１４－１　フィルム
　１６ａ、１６ｂ　カーボンインク
　１６ａ－１　金属膜
　２２　微細溝
　２２’　流路
　２３ａ、２３ｂ、２４ａ、２４ｂ、２７　貫通孔
　２３ａ’、２３ｂ’、２６’　第１領域
　２４ａ’、２４ｂ’　第２領域
　２５ａ、２５ｂ　凹み
　２５ａ’、２５ｂ’　第３領域
　２６ａ’、２６ｂ’、２６－１　接着剤
　２７’　注入口

Claims (3)

  1. 　凹みまたは貫通孔が形成された基板部材と、
    　前記基板部材の一方の面に接合された、薄膜状または薄板状の蓋部材と、
    　前記蓋部材の前記基板部材側の面の一部を覆うように層状に形成された、電気または熱を伝達する伝達機能部と、を有し、
    　前記基板部材の前記伝達機能部の一端に対応する部分には、第１領域を形成する凹みまたは貫通孔が形成されており、
    　前記第１領域を形成する凹みまたは貫通孔の前記蓋部材側の開口部は、前記蓋部材により閉塞されており、
    　前記基板部材の前記伝達機能部の他端に対応する部分には、外部に連通する第２領域が形成されており、
    　前記伝達機能部は、前記第１領域と前記第２領域との間を電気的または熱的に接続しており、
    　前記基板部材の前記伝達機能部の前記一端および前記他端との間の部分に対応する部分には、第３領域を形成する凹みが前記伝達機能部の縁部に架かるように形成されており、
    　前記第３領域を形成する凹みの前記蓋部材側の開口部は、前記蓋部材により閉塞されており、
    　前記第３領域は、前記第２領域と連通しており、かつ接着剤で充填されている、
    　流体取扱装置。
  2. 　前記接着剤が、導電性または伝熱性を有する、請求項１に記載の流体取扱装置。
  3. 　請求項１に記載の流体取扱装置を具備する流体取扱システム。
PCT/JP2012/001690 2011-04-04 2012-03-12 流体取扱装置及び流体取扱システム Ceased WO2012137413A1 (ja)

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