WO2012135591A3 - Production microbienne d'isoprénoïdes à l'aide de voie dxp hétérologue - Google Patents

Production microbienne d'isoprénoïdes à l'aide de voie dxp hétérologue Download PDF

Info

Publication number
WO2012135591A3
WO2012135591A3 PCT/US2012/031395 US2012031395W WO2012135591A3 WO 2012135591 A3 WO2012135591 A3 WO 2012135591A3 US 2012031395 W US2012031395 W US 2012031395W WO 2012135591 A3 WO2012135591 A3 WO 2012135591A3
Authority
WO
WIPO (PCT)
Prior art keywords
heterologous
dxp pathway
isoprenoid production
microbial
microbial isoprenoid
Prior art date
Application number
PCT/US2012/031395
Other languages
English (en)
Other versions
WO2012135591A2 (fr
Inventor
Lishan Zhao
Kevin L. DIETZEL
Gale A. WICHMANN
Original Assignee
Amyris, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris, Inc. filed Critical Amyris, Inc.
Publication of WO2012135591A2 publication Critical patent/WO2012135591A2/fr
Publication of WO2012135591A3 publication Critical patent/WO2012135591A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne des procédés qui permettent une production robuste d'isoprénoïdes à l'aide de la voie de biosynthèse de DXP. L'invention concerne également des acides nucléiques, des enzymes, des vecteurs d'expression et des cellules hôtes génétiquement modifiées pour la mise en œuvre des procédés de l'invention.
PCT/US2012/031395 2011-03-30 2012-03-30 Production microbienne d'isoprénoïdes à l'aide de voie dxp hétérologue WO2012135591A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161469767P 2011-03-30 2011-03-30
US61/469,767 2011-03-30

Publications (2)

Publication Number Publication Date
WO2012135591A2 WO2012135591A2 (fr) 2012-10-04
WO2012135591A3 true WO2012135591A3 (fr) 2014-05-01

Family

ID=46932358

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/031395 WO2012135591A2 (fr) 2011-03-30 2012-03-30 Production microbienne d'isoprénoïdes à l'aide de voie dxp hétérologue

Country Status (1)

Country Link
WO (1) WO2012135591A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9920312B2 (en) * 2014-08-11 2018-03-20 Lallemand Hungary Liquidity Management Llc Mutations in iron-sulfur cluster proteins that improve xylose utilization
KR102331018B1 (ko) 2016-08-12 2021-11-26 아미리스 인코퍼레이티드 레바우디오시드의 고효율 생산을 위한 udp 의존성 글리코실트랜스퍼라제
CN108624516B (zh) * 2017-03-20 2022-08-26 华东理工大学 一种提高发酵细胞中的代谢产物量及制备idms标准品的方法
CN109234216B (zh) * 2017-07-10 2022-05-20 上海医药工业研究院 一种生产鲨烯的基因工程菌及其方法
WO2019033064A1 (fr) 2017-08-11 2019-02-14 Amyris, Inc. Kaurène oxydase de pisum sativum pour la production à haut rendement de rebaudiosides
KR20200095534A (ko) 2017-12-07 2020-08-10 지머젠 인코포레이티드 발효에 의한 (6e)-8-히드록시제라니올의 제조를 위해 조작된 생합성 경로
CA3086490A1 (fr) 2017-12-21 2019-06-27 Zymergen Inc. Nepetalactol oxydoreductases, nepetalactol synthases et microbes capables de produire une nepetalactone
CN110468089B (zh) * 2018-05-09 2023-07-11 深圳艾格鑫科技有限公司 微生物及其用途
CN108715825B (zh) * 2018-06-01 2021-03-09 天津大学 基因过表达及获得的菌株、应用
WO2020081468A1 (fr) 2018-10-15 2020-04-23 Amyris, Inc. Variants d'hydroxylase d'acide kaurénoïque de stevia rebaudiana pour la production à haut rendement de rebaudiosides
CN113631698A (zh) 2019-01-24 2021-11-09 阿迈瑞斯公司 用于高效生成瑞鲍迪苷的abc转运蛋白

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090275104A1 (en) * 2004-01-09 2009-11-05 Novozymes A/S Bacillus licheniformis chromosone
US20110014672A1 (en) * 2009-06-17 2011-01-20 Chotani Gopal K Isoprene production using the dxp and mva pathway

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090275104A1 (en) * 2004-01-09 2009-11-05 Novozymes A/S Bacillus licheniformis chromosone
US20110014672A1 (en) * 2009-06-17 2011-01-20 Chotani Gopal K Isoprene production using the dxp and mva pathway

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FARHI ET AL.: "Harnessing yeast subcellular compartments for the production of plant terpenoids.", METABOLIC ENGINEERING, vol. 13, 18 May 2011 (2011-05-18), pages 474 - 481, XP028274814, DOI: doi:10.1016/j.ymben.2011.05.001 *
MAURY ET AL.: "Reconstruction of a bacterial isoprenoid biosynthetic pathway in Saccharomyces cerevisiae.", FEBS LETT, vol. 582, 2008, pages 4032 - 4038, XP025745917, DOI: doi:10.1016/j.febslet.2008.10.045 *
NETZ ET AL.: "The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol.", NATURE CHEMICAL BIOLOGY, vol. 3, 2007, pages 278 - 286 *

Also Published As

Publication number Publication date
WO2012135591A2 (fr) 2012-10-04

Similar Documents

Publication Publication Date Title
WO2012135591A3 (fr) Production microbienne d'isoprénoïdes à l'aide de voie dxp hétérologue
CA2863405A1 (fr) Nouvelle classe de genes de resistance au glyphosate
WO2012058494A3 (fr) Variantes d'isoprène synthase pour l'augmentation de la production d'isoprène
WO2011153246A3 (fr) Extraction d'un lipide à partir de cellules et produits obtenus à partir de cette extraction
MX355504B (es) Éster de cera de proteína portadora de acil-acil sintasas.
WO2011150241A3 (fr) Réduction du niveau de lactate et augmentation de la production de polypeptides par régulation négative de l'expression de la lactate déshydrogénase et de la pyruvate déshydrogénase kinase
EP2818543B8 (fr) Bacille, hyaluronidase et leurs applications
EP3543333A3 (fr) Procédé de criblage d'alpha-amylases
WO2011153300A3 (fr) Méthodes de détection d'anticorps monoclonaux pour des enzymes qui confèrent à des végétaux une résistance à l'acide 2,4-dichlorophénoxyacétique
MX2013001045A (es) Organoide hepatico, sus usos y metodo de cultivo para obtenerlos.
WO2011082425A3 (fr) Variants d'alpha-amylase et polynucleotides les codant
WO2009042070A3 (fr) Production d'isoprénoïdes
WO2013082186A3 (fr) Souches microbiennes génétiquement modifiées comprenant des gènes de la voie des lipides de prototheca
WO2014022702A3 (fr) Procédés et compositions permettant de réguler l'expression génique par maturation de l'arn
MX346518B (es) Metodos de desarrollo de variantes de terpeno sintasa.
WO2012019002A3 (fr) Cellules souches somatiques
MX349801B (es) Sustratos de enzimas de ácido nucleico.
EP3696265A3 (fr) Variantes de la lipase et polynucléotides les codant
WO2011160052A3 (fr) Procédés et compositions associés à des arn-endonucléases spécifiques d'une séquence
WO2012082731A3 (fr) Microalgues modifiées avec production de lipides augmentée
WO2014014988A3 (fr) Amorces coopératives, sondes et applications correspondantes
WO2012177983A3 (fr) Microorganismes capables de produire de l'éthylène glycol et procédés associés
ZA201403705B (en) Methods for the preparation of hydrogels using lipase enzymes
WO2014066903A8 (fr) Lutte biologique contre les nématodes
WO2013096683A3 (fr) Augmentation de la production d'isoprène avec des cellules bactériennes marines

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12765659

Country of ref document: EP

Kind code of ref document: A2

122 Ep: pct application non-entry in european phase

Ref document number: 12765659

Country of ref document: EP

Kind code of ref document: A2