WO2012118318A3 - 비천연 아미노산 생산 균주 및 이를 이용한 비천연 아미노산의 제조 방법 - Google Patents

비천연 아미노산 생산 균주 및 이를 이용한 비천연 아미노산의 제조 방법 Download PDF

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WO2012118318A3
WO2012118318A3 PCT/KR2012/001488 KR2012001488W WO2012118318A3 WO 2012118318 A3 WO2012118318 A3 WO 2012118318A3 KR 2012001488 W KR2012001488 W KR 2012001488W WO 2012118318 A3 WO2012118318 A3 WO 2012118318A3
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amino acids
microorganism
producing non
natural amino
producing
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PCT/KR2012/001488
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English (en)
French (fr)
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WO2012118318A2 (ko
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박진병
김주연
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이화여자대학교 산학협력단
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Publication of WO2012118318A2 publication Critical patent/WO2012118318A2/ko
Publication of WO2012118318A3 publication Critical patent/WO2012118318A3/ko

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 비천연 아미노산 생산 균주에 관한 것으로, 보다 구체적으로 트랜스아미나제를 코딩하는 유전자가 도입된 비천연 아미노산 생산용 코리네박테리움속 균주, 상기 균주를 제조하는 방법, 상기 균주를 이용하여 비천연 아미노산을 제조하는 방법 및 상기 균주의 비천연 아미노산 생산 용도에 관한 것이다. 본 발명의 비천연 아미노산 생산용 균주는 일반적인 생물전환 중 발생하는 알파키토글루타레이트를 추가 비용 없이 저농도로 유지하고, 생물전환에 사용되는 글루탐산을 효율적으로 생산하는 코리네박테리움 속 균주를 이용하여 효율적이며 간편하게 원하는 비천연 아미노산을 제조할 수 있다.
PCT/KR2012/001488 2011-02-28 2012-02-28 비천연 아미노산 생산 균주 및 이를 이용한 비천연 아미노산의 제조 방법 WO2012118318A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0018065 2011-02-28
KR1020110018065A KR20120098235A (ko) 2011-02-28 2011-02-28 비천연 아미노산 생산 균주 및 이를 이용한 비천연 아미노산의 제조 방법

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WO2012118318A2 WO2012118318A2 (ko) 2012-09-07
WO2012118318A3 true WO2012118318A3 (ko) 2012-12-13

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PCT/KR2012/001488 WO2012118318A2 (ko) 2011-02-28 2012-02-28 비천연 아미노산 생산 균주 및 이를 이용한 비천연 아미노산의 제조 방법

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KR (1) KR20120098235A (ko)
WO (1) WO2012118318A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109609478B (zh) * 2019-01-14 2020-06-16 浙江工业大学 α-转氨酶及突变体以及在不对称合成L-草铵膦中的应用
KR102143964B1 (ko) * 2019-12-06 2020-08-12 씨제이제일제당 주식회사 신규한 분지쇄 아미노산 아미노트랜스퍼라제 변이체 및 이를 이용한 류신 생산방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1335658C (en) * 1986-06-04 1995-05-23 Johann Then Process for preparing l-tertiary-leucine and l-phosphinothricine by transamination
US5728555A (en) * 1996-09-30 1998-03-17 Monsanto Company Preparation of d-amino acids by direct fermentative means
US6197558B1 (en) * 1997-05-19 2001-03-06 Nsc Technologies Transaminase biotransformation process

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1335658C (en) * 1986-06-04 1995-05-23 Johann Then Process for preparing l-tertiary-leucine and l-phosphinothricine by transamination
US5728555A (en) * 1996-09-30 1998-03-17 Monsanto Company Preparation of d-amino acids by direct fermentative means
US6197558B1 (en) * 1997-05-19 2001-03-06 Nsc Technologies Transaminase biotransformation process

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
EUN YOUNG HONG ET AL.: "Asymmetric synthesis of L-tert-leucine and L-3- hydroxyadamantylglycine using branched chain aminotransferase", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 66, June 2010 (2010-06-01), pages 228 - 233, XP027145678 *

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WO2012118318A2 (ko) 2012-09-07
KR20120098235A (ko) 2012-09-05

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