WO2012108669A3 - Uranyl ion specific dna aptamer - Google Patents

Uranyl ion specific dna aptamer Download PDF

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Publication number
WO2012108669A3
WO2012108669A3 PCT/KR2012/000889 KR2012000889W WO2012108669A3 WO 2012108669 A3 WO2012108669 A3 WO 2012108669A3 KR 2012000889 W KR2012000889 W KR 2012000889W WO 2012108669 A3 WO2012108669 A3 WO 2012108669A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna aptamer
specific dna
ion specific
uranyl ion
uranyl
Prior art date
Application number
PCT/KR2012/000889
Other languages
French (fr)
Korean (ko)
Other versions
WO2012108669A2 (en
Inventor
하상수
김지수
Original Assignee
경희대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 경희대학교 산학협력단 filed Critical 경희대학교 산학협력단
Publication of WO2012108669A2 publication Critical patent/WO2012108669A2/en
Publication of WO2012108669A3 publication Critical patent/WO2012108669A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer

Abstract

Provided is a uranyl ion specific DNA aptamer. Also provided is a uranyl ion specific DNA aptamer which is bound to a solid support. Also provided is a method for isolating uranyl ions from a sample by using the uranyl ion specific DNA aptamer. The DNA aptamer of the present invention binds to uranyl ions with high specificity, such that uranium can be efficiently extracted from marine and other such environments.
PCT/KR2012/000889 2011-02-08 2012-02-07 Uranyl ion specific dna aptamer WO2012108669A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110010928A KR101238297B1 (en) 2011-02-08 2011-02-08 DNA aptamer specific for uranyl ion
KR10-2011-0010928 2011-02-08

Publications (2)

Publication Number Publication Date
WO2012108669A2 WO2012108669A2 (en) 2012-08-16
WO2012108669A3 true WO2012108669A3 (en) 2012-12-20

Family

ID=46639044

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/000889 WO2012108669A2 (en) 2011-02-08 2012-02-07 Uranyl ion specific dna aptamer

Country Status (2)

Country Link
KR (1) KR101238297B1 (en)
WO (1) WO2012108669A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102248839B1 (en) * 2019-12-09 2021-05-06 충북대학교 산학협력단 Dna aptamer specifically binding to lithium and using the same
KR102328488B1 (en) 2021-06-24 2021-11-17 세종대학교산학협력단 Aptamer specifically binding to at least one of a nickel ion and a cobalt ion
CN113773444B (en) * 2021-09-08 2022-04-26 北京大学 Material and method for recycling trace uranium from seawater
KR102378495B1 (en) 2021-11-01 2022-03-23 세종대학교산학협력단 Aptamer specifically binding to a nickel ion
KR20240054446A (en) 2022-10-18 2024-04-26 세종대학교산학협력단 Aptamer specifically binding to a manganese ion

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100009031A (en) * 2008-07-17 2010-01-27 김양훈 Dna aptamer specifically binding to arsenic and uses thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9273345B2 (en) 2006-12-28 2016-03-01 Conceptual Mindworks, Inc. Methods and compositions for processes of rapid selection and production of nucleic acid aptamers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100009031A (en) * 2008-07-17 2010-01-27 김양훈 Dna aptamer specifically binding to arsenic and uses thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BREAKER, R. R. ET AL.: "DNA aptamers and DNA enzymes", CURRENT OPINION IN CHEMICAL BIOLOGY., vol. 1, no. 1, June 1997 (1997-06-01), pages 26 - 31 *
KIM, J. ET AL.: "Binding or uranyl ion by a DNA aptamer attached to a solid support", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS., vol. 21, no. 13, 6 May 2011 (2011-05-06), pages 4020 - 4022 *
LIU, J. ET AL.: "A catalytic beacon sensor for uranium with parts-per-trillion sensitivity and millionfold selectivity", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE, vol. 104, no. 7, 6 February 2007 (2007-02-06), pages 2056 - 2061 *
STOLTENBURG, R. ET AL.: "SELEX- a (r)evolutionary method to generate high-affinity nucleic acid ligands", BIOMOLECULAR ENGINEERING., vol. 24, no. 4, 16 June 2007 (2007-06-16), pages 381 - 403 *

Also Published As

Publication number Publication date
KR101238297B1 (en) 2013-03-04
KR20120090477A (en) 2012-08-17
WO2012108669A2 (en) 2012-08-16

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