WO2012104667A1 - Traitement en mode continu de culture d'algues eucaryotes et système associé de photo-bioréacteur - Google Patents

Traitement en mode continu de culture d'algues eucaryotes et système associé de photo-bioréacteur Download PDF

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Publication number
WO2012104667A1
WO2012104667A1 PCT/IB2011/000181 IB2011000181W WO2012104667A1 WO 2012104667 A1 WO2012104667 A1 WO 2012104667A1 IB 2011000181 W IB2011000181 W IB 2011000181W WO 2012104667 A1 WO2012104667 A1 WO 2012104667A1
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WO
WIPO (PCT)
Prior art keywords
algae
bio
growth
algae culture
transparent
Prior art date
Application number
PCT/IB2011/000181
Other languages
English (en)
Inventor
Krisada Kampanatsanyakorn
Suradit Holasut
Original Assignee
Krisada Kampanatsanyakorn
Suradit Holasut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Krisada Kampanatsanyakorn, Suradit Holasut filed Critical Krisada Kampanatsanyakorn
Priority to PCT/IB2011/000181 priority Critical patent/WO2012104667A1/fr
Publication of WO2012104667A1 publication Critical patent/WO2012104667A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus

Definitions

  • the present invention relates generally to eukaryotic micro algae farming and in particular to a continuous mode process and related photo-bio-reactor system.
  • bio-fuels are categorized as first generation bio- fuels when the feedstock used may be classified as food crops such as for example sugar, starch, vegetable oils and animal fats, whilst second generation bio-fuels are those generated with non-food.
  • a traditional conversion process is anaerobic digestion of organic wastes.
  • a by-product of anaerobic digestion processes is carbon dioxide (C0 2 ) which is a greenhouse gas.
  • Algae are generally rich of oil that is convertible into bio-fuel and in order to grow they require water, light and carbon dioxide: that is, differently from anaerobic digestion, growing algae fixes the carbon of dissolved carbon dioxide in the growth liquor instead of producing it as by-product and require a source of it.
  • algae may be grown in open environment as in a tank or pond, efforts are made for devising cost efficient closed environment systems in form of photo- bio-reactors in which farming algae in an efficient manner.
  • micro algae growing systems are associated to first generation bio-fuel conversion plants or to thermal generation plants in order to sinergically utilize part or all carbon dioxide produced as by-product by the anaerobic digestion or by the burning a fuel and abate/reduce release of carbon dioxide in the atmosphere.
  • batch production remains the general approach or account of certain specific characteristics attributed to the growth process of these vegetable organisms.
  • the applicants have devised an improved method and system for growing eukaryotic algae in a substantially closed environment employing a photo-bio- reactor, in a substantially continuous mode while significantly reducing the liquid mass to be handled.
  • the novel system greatly simplifies and makes more reliable a constant control of the critical parameters of a continuously circulating liquid algae culture (growth liquor) an enhances yield of bio mass that can be efficiently bled from the system continuously or at frequent intervals of time, in form of a relatively dense mass of algae settled at the bottom of a lower termination of an opaque vessel of the photo-bio-reactor system where the growth liquor flows for being re-circulated again and again through illuminated transparent conduits connected thereto, in form of a conical, pyramidal or triangular cross section deep trough terminating with a bleed valve.
  • Another likely reason of the surprisingly high yield obtained may be because the algae tend to continue or complete a light-promoted metabolic growth process for part of the transit time in the unlit interior of the opaque vessel of the photo-bio- reactor circuit, while circulating back to the inlet of the illuminated transparent conduit or conduits.
  • Figure 1 is a basic scheme of the disclosed system.
  • FIG. 1 is a basic scheme of the core part of a system for growing microalgae according to the present disclosure.
  • the photo-bio-reactor circuit of a liquid algae culture also referred to as growth liquor, is composed of one or several salient transparent flow conduits, for example in form of transparent pipes TP exposed to solar or artificial light, and an upright opaque vessel T-3 (that may and is often referred to with a functional definition such as "decanter” or "settler") having a bottom extension BT, below the connection to the transparent pipe, in the form of a deep settling trough or of an array of troughs.
  • the transparent pipes TP may extend straight up or, as shown in the figure, they may branch out in a radial direction, with joined outward and inward sloping parts in order to increase the length of the illuminated flow path of the growth liquor in the conduit so composed, that is to say the transit time for a given flow velocity of the growth liquor in the illuminated conduit.
  • the settler vessel T-3 may be a upright cylindrical vessel having a single conical trough BT at its base, and to which a plurality of "salient loops" of transparent pipes TP, as the one illustrated in the figure, are connected at angular intervals around the vessel circumference.
  • the settler T-3 may be an elongated rectangular base upright vessel with a single trough of triangular cross section at its base, equipped with several uniformly spaced bleed or discharge valves DV, or with a plurality of distinct pyramidal troughs at the bottom each with its own bleed valve DV, and to which a bank of parallel salient transparent pipe loops TP as the single one illustrated in the figure are connected at intervals along one or even along both longest sides of the elongated rectangular cross sectional vessel.
  • Recirculation motion of the growth liquor may be implemented as shown in the exemplary embodiment of FIG. 1, by injecting a gas or a gas mixture, for example compressed air, preferably though not necessarily, through a porous septum (bubbler), at the bottom of each transparent pipe loop, for gas lifting the growth liquor inside the salient transparent conduit.
  • a gas or a gas mixture for example compressed air
  • the gas injected in the lower part of the circulation circuit of the growth liquor may be vented off to atmosphere at the top of the settler T-3 or collected and re-cycled back to the injection port after re-compressing it or, as yet another alternative embodiment of the process of this disclosure, it may be conveyed to a heat-engine, depending from the gas or gas mixture that is employed for gas lifting the liquor in the illuminated conduits.
  • a bleed or discharge valve DV at the bottom of the single trough BT or of every trough of the settler vessel T-3, permits to continuously or intermittently bleed from the photo-bio-reactor system a wet mass of algae that have settled inside the bottom trough of the recirculation vessel.
  • the so continuously harvested wet mass of algae is much more concentrated then in the growth liquor that circulates in the transparent illuminated pipes and in the opaque settler.
  • the concentration of algae harvested continuously or intermittently at regular intervals through bleed valves of a dense wet mass settled at the bottom of the trough or troughs is from about five to about ten times or even more the concentration of algae in the liquid moving above the rim of the settling trough that, according to the novel continuous process of this disclosure, can be effectively kept within an optimal concentration range of about 10 to about 40 grams of algae per liter of the circulating growth liquor.
  • this condition is achieved and maintained without significantly increasing the volume of ad the residence time of the growth liquor in an unlit tank in order to achieve an effective decantation of algae.
  • the smaller sized ancillary tanks, T-l and T-2 illustrated in the figure, facilitate preparation and introduction in the photo-bio-reactor circuit of make-up quantities of growth liquid, for example by mixing water with appropriate nutrients in one while the other of the two provides a source of make-up liquid in order to keep constant the liquid level in the settler vessel T-3 while withdrawing settled wet mass of algae from the bottom of the harvesting trough.
  • the liquid recovered when dewatering the harvested wet mass of algae containing nutrients and seeds of the algae is re-cycled back into one of the two ancillary tanks, T-l and T-2.
  • liquid level sensing means SI control a make-up pump PI .
  • the pH of the alkaline growth liquor has great influence on the growth rate of the microalgae.
  • the various species being commonly farmed have a specific range of pH for growing at the maximum rate at parity of other growth factors such as spectrum and intensity of the illuminating light, availability of nutrients in the growth liquor, temperature, mechanical stresses etc..
  • Chlorella the maximum growth rate is achieved at a pH comprised between 8.0 and 8.5.
  • the pH is automatically regulated by injecting carbon dioxide mixed with the gas or gas mixture used for gas lifting the growth liquor when the pH surpasses the upper limit of the optimal range, and the carbon dioxide injection ceases when the pH drops to the lower limit of the optimal range.
  • a pH sensor may be installed at the top of a salient transparent pipe loop, for implementing an on/off control of an electro-valve EV that releases carbon dioxide in the gas stream coming from a compressor C-l.
  • a flow rate adjustment needle valve up-stream of the electro- valve EV will help reducing the frequency of on/off switching of the electro- valve.
  • the added carbon dioxide transforms carbonates generally present in the growth liquor into bicarbonates thus lowering the pH.
  • the growing microalgae assume carbon from dissolved bicarbonate and not directly from the injected carbon dioxide.
  • Movement of algae in the growth liquor is important for achieving a high growth rate. Stillness or scarce motion lowers the growth rate because the algae may consume the nutrients faster than they can be replaced by fresh nutrients by diffusion alone. Therefore a continuous mixing, normally implemented by imposing a turbulent flow to the growth liquor through an illuminated transparent conduit is an established common practice.
  • microalgae are subjected to abrupt accelerations they may be damaged and generally suffer from severe impacts among each other.
  • a Reynolds number in a range of about 10000 to about 80000, has been found to be optimal for Chlorella.
  • a compressed air flow rate of about 20 liters/minute produces a liquid velocity of about 20 cm/sec, well within the above range of Reynolds number, such to ensure turbulent flow conditions without excessively stressing the growing organisms.
  • concentration of microalgae in the growth liquor circulating into the illuminated transparent conduits TP has a prominent role on the growth rate by influencing the mean intensity of illumination of the whole population of algae that is being apparently exposed to light when flowing into a transparent tube, the diffusive processes of nutrients in the algae-laden growth liquor, effectiveness of turbulence in promoting mass transport of nutrients in the liquor and the mechanical impact mechanism of stress/damage of the growing organisms.
  • growth rate can be expressed in percentage of weight increase in a given time (for example a growth rate of 3% to 10% per day). If the population is scarce also the overall weight increase will be low.
  • pH control can be carried out accurately within a very narrow limits operating always in the region of maximum grow rate.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne la croissance d'une algue eucaryote dans un environnement sensiblement fermé employant un photo-bioréacteur en mode sensiblement continu tout en réduisant de manière significative la masse liquide à gérer. Le nouveau système simplifie grandement et fiabilise un contrôle constant des paramètres critiques d'une culture d'algues liquides à circulation continue(liqueur de croissance) et améliore le rendement d'une biomasse qui peut être efficacement purgée du système de manière continue à intervalles de temps fréquents, sous la forme d'une masse relativement dense d'algues colonisées au fond d'une terminaison inférieure d'un flacon opaque du système de photo-bioréacteur dans lequel la liqueur de croissance s'écoule pour être remise en circulation encore et encore dans des conduits transparents éclairés qui y sont reliés, sous forme de rigole profonde à section transversale conique, pyramidale ou triangulaire se terminant par une soupape de purge.
PCT/IB2011/000181 2011-02-04 2011-02-04 Traitement en mode continu de culture d'algues eucaryotes et système associé de photo-bioréacteur WO2012104667A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IB2011/000181 WO2012104667A1 (fr) 2011-02-04 2011-02-04 Traitement en mode continu de culture d'algues eucaryotes et système associé de photo-bioréacteur

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2011/000181 WO2012104667A1 (fr) 2011-02-04 2011-02-04 Traitement en mode continu de culture d'algues eucaryotes et système associé de photo-bioréacteur

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WO2012104667A1 true WO2012104667A1 (fr) 2012-08-09

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102945034A (zh) * 2012-11-09 2013-02-27 江苏元升太阳能集团有限公司 一种太阳能空气源热泵热水可视化远程监控系统
US20160272524A1 (en) * 2013-11-07 2016-09-22 Niels Holm Method for Wastewater Treatment by Means of Mixed Algae Cultures that Sediment and Device for Performing Said Method
CN108138103A (zh) * 2015-09-30 2018-06-08 Subitec有限责任公司 具有可中断的气体供应的生物反应器
JP2018532429A (ja) * 2015-10-26 2018-11-08 ロンザ リミテッド バイオ医薬品を生産するための製造設備
WO2023282762A1 (fr) * 2021-07-05 2023-01-12 Mood Harvest As Équipements et installations de production pour la culture

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2558659Y (zh) * 2002-06-26 2003-07-02 江门市蓬江区海豚水族有限公司 自动化连续生产跑道式光生物反应器
CN2688718Y (zh) * 2004-02-27 2005-03-30 华南理工大学 螺旋管式光生物反应器
US20070231886A1 (en) * 2006-03-28 2007-10-04 Sartorius Ag Reactor plant and process for culturing phototropic microorganisms
CN101353619A (zh) * 2007-07-27 2009-01-28 卢朝辉 藻类微生物光合反应系统
WO2011008784A2 (fr) * 2009-07-13 2011-01-20 Inventure Chemical, Inc. Procédé pour récolter des micro-algues en suspension dans une solution aqueuse à l'aide d'un produit chimique hydrophobe

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2558659Y (zh) * 2002-06-26 2003-07-02 江门市蓬江区海豚水族有限公司 自动化连续生产跑道式光生物反应器
CN2688718Y (zh) * 2004-02-27 2005-03-30 华南理工大学 螺旋管式光生物反应器
US20070231886A1 (en) * 2006-03-28 2007-10-04 Sartorius Ag Reactor plant and process for culturing phototropic microorganisms
CN101353619A (zh) * 2007-07-27 2009-01-28 卢朝辉 藻类微生物光合反应系统
WO2011008784A2 (fr) * 2009-07-13 2011-01-20 Inventure Chemical, Inc. Procédé pour récolter des micro-algues en suspension dans une solution aqueuse à l'aide d'un produit chimique hydrophobe

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102945034A (zh) * 2012-11-09 2013-02-27 江苏元升太阳能集团有限公司 一种太阳能空气源热泵热水可视化远程监控系统
US20160272524A1 (en) * 2013-11-07 2016-09-22 Niels Holm Method for Wastewater Treatment by Means of Mixed Algae Cultures that Sediment and Device for Performing Said Method
US9932254B2 (en) 2013-11-07 2018-04-03 Niels Holm Method for wastewater treatment by means of mixed algae cultures that sediment and device for performing said method
CN108138103A (zh) * 2015-09-30 2018-06-08 Subitec有限责任公司 具有可中断的气体供应的生物反应器
JP2018532429A (ja) * 2015-10-26 2018-11-08 ロンザ リミテッド バイオ医薬品を生産するための製造設備
WO2023282762A1 (fr) * 2021-07-05 2023-01-12 Mood Harvest As Équipements et installations de production pour la culture

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