WO2012101733A1 - Powdery soybean material and edible composition using same - Google Patents

Powdery soybean material and edible composition using same Download PDF

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WO2012101733A1
WO2012101733A1 PCT/JP2011/051170 JP2011051170W WO2012101733A1 WO 2012101733 A1 WO2012101733 A1 WO 2012101733A1 JP 2011051170 W JP2011051170 W JP 2011051170W WO 2012101733 A1 WO2012101733 A1 WO 2012101733A1
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protein
soybean
soy
edible composition
dietary fiber
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PCT/JP2011/051170
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French (fr)
Japanese (ja)
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佐本 将彦
釘谷 博文
千晶 宮崎
司 木山
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不二製油株式会社
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Priority to PCT/JP2011/051170 priority Critical patent/WO2012101733A1/en
Publication of WO2012101733A1 publication Critical patent/WO2012101733A1/en

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    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D2/00Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/08Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
    • A21D2/24Organic nitrogen compounds
    • A21D2/26Proteins
    • A21D2/264Vegetable proteins
    • A21D2/266Vegetable proteins from leguminous or other vegetable seeds; from press-cake or oil bearing seeds
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D2/00Treatment of flour or dough by adding materials thereto before or during baking
    • A21D2/08Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
    • A21D2/36Vegetable material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/05Mashed or comminuted pulses or legumes; Products made therefrom
    • A23L11/07Soya beans, e.g. oil-extracted soya bean flakes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/424Addition of non-meat animal protein material, e.g. blood, egg, dairy products, fish; Proteins from microorganisms, yeasts or fungi
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/185Vegetable proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21Addition of substantially indigestible substances, e.g. dietary fibres
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • A23L7/157Farinaceous granules for dressing meat, fish or the like
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates to a powdery soybean material and an edible composition using the same.
  • it is related with the edible composition using the powdery soybean raw material using the specific fraction taken out from soybean, and the function peculiar to this soybean raw material.
  • Soy protein is widely used for improving the physical properties of edible compositions because of its unique gelling power, and is also increasingly used as a health food material with high nutritional value as a protein supply source.
  • Soybean storage protein precipitates around pH 4.5 and can be divided into a soybean whey protein fraction mainly composed of acid-soluble components other than the storage protein and an acid-precipitated protein fraction mainly composed of storage protein. it can.
  • a product obtained by collecting this acid-precipitating protein fraction is a separated soy protein, which has a high gel forming ability and a high amino acid score nutritionally, and is currently widely used in the food industry.
  • Proteins constituting soy protein are classified into 2S, 7S, 11S, and 15S proteins based on the sedimentation coefficient obtained by ultracentrifugation analysis.
  • 7S protein and 11S protein are the main constituents of soy acid-precipitating protein.
  • ⁇ -conglycinin substantially corresponds to the 7S protein
  • glycinin substantially corresponds to the 11S protein.
  • 7S protein has been reported to nutritionally reduce neutral fat in blood (Non-patent Document 1).
  • the gel shows an elastic gel and the liquid is highly viscous.
  • 11S protein has a high gelling power and is said to control the hardness and texture of tofu gel.
  • both 7S protein and 11S protein are characterized by relatively strong gel-forming ability, but their physical properties differ depending on processing conditions. For example, when heating in a system with a large amount of water, the texture changes due to the characteristics of the 7S protein, and the texture changes, and the cohesive strength is strong as a result of the heating behavior of the 11S protein to acids, Ca and Mg ions. Changes in taste and roughness due to being born can be felt, and it may not always be preferable as an edible composition.
  • Non-patent Document 2 Non-patent Document 2
  • This lipophilic protein is recognized to have a cholesterol-reducing effect (Patent Document 1) and a nephropathy-preventing effect (Patent Document 2), and a specific nutritional function is being clarified.
  • Patent Document 1 the physical properties of the lipophilic protein are not known in detail.
  • Patent Document 3 emulsifies a complex of oleosin and phospholipid, which is one of lipophilic proteins.
  • a technique for use as a stabilizer is disclosed.
  • lipophilic proteins are complexed in this way and accompanied by lipids, so that they are easily oxidized and are said to cause off-flavour. For this reason, it is considered that the food material has a bad flavor and is difficult to use, and it is still far from being used for the practical use of the nutritional function.
  • the present invention is able to take advantage of characteristic nutritional functional properties such as cholesterol-reducing effects and nephropathy-preventing effects possessed by lipophilic proteins, and is excellent in processability as a food material. It is an object of the present invention to provide a powdered soybean material that can add a nutritional function without impairing the flavor and texture of the composition. In addition, the present invention provides a powdered soy material having superior processability, which is not found in conventional soy materials, and provides an edible composition that takes advantage of characteristic physical properties, regardless of the nutritional functional characteristics of lipophilic proteins. Is an issue.
  • the present inventors fractionated lipophilic proteins and investigated in detail their physical properties and flavors.
  • the single lipophilic protein was found to have a 7S protein having a gel formability and a cohesive strength. It was found to be weaker than the 11S protein.
  • the inventors have found the possibility of utilizing the characteristics of the above and have solved the above problems.
  • a powdery soy material is mainly composed of a lipophilic protein with little gel-forming ability and cohesive force and soy dietary fiber, and various edible compositions for such material. It was found that it can be used to improve the physical properties of materials.
  • conventional materials mainly composed of soy dietary fiber such as Okara have water retention, but their shape retention is weak because of their low binding and cohesiveness. In many cases, it felt like being sucked up.
  • the present invention has found a material having both a shape retaining property with an appropriate binding force and a smooth texture and emulsifying property by combining a lipophilic protein and soybean dietary fiber.
  • the present invention (1) A powdered soy material comprising soy protein and soy dietary fiber, wherein the protein content is 35 to 85% by weight per non-fat solid content, and the LCI value of the protein is 60 or more, (2) The powdered soybean material according to (1), wherein the dietary fiber content of soybean is 15 to 130% by weight based on protein, (3) An edible composition using the powdered soybean material according to (1), (4) The edible composition according to (3), which is a powdered composition, an emulsified composition, a baked or fried confectionery, a kneaded product, or a semi-solid or gel-like composition, (5) The edible composition according to the above (4), wherein the edible composition is a protein or dietary fiber reinforced.
  • a powdered soy material containing soy protein and soy dietary fiber, having a protein content of 35 to 85% by weight of the non-fat solid content and a protein LCI value of 60 or more is contained in the edible composition.
  • a method for improving the physical properties of an edible composition characterized by comprising: (7) The method for improving physical properties of an edible composition according to the above (6), wherein the physical properties are dispersibility, shape retention, water retention, emulsion stability, oxidation stability or texture.
  • various good physical properties such as good texture, dispersibility of edible compositions in raw material dough, good shape retention, etc., which were difficult to obtain with conventional soybean materials such as isolated soybean protein and concentrated soybean protein, etc.
  • the powdery soybean material which has can be provided.
  • it is possible to improve the conventional drawbacks related to the physical properties of the edible composition, and to nutritionally strengthen proteins and dietary fibers in the edible composition without deteriorating the original physical properties of the edible composition.
  • it can be provided as a raw material for reinforcing protein or dietary fiber used in edible compositions for the purpose of manifesting physiological functions, such as cholesterol-reducing effects, nephropathy-preventing effects, intestinal environment-improving effects, and the like. In the case of things, it becomes possible to reinforce protein or dietary fiber without damaging the physical properties.
  • the first well from the left is a molecular weight marker
  • the second to fifth wells from the left are standard samples for preparing a calibration curve for calculating the concentration of MSP
  • the first and second wells from the right are measurement samples.
  • 7S protein / 11S protein / acid-precipitating protein 7S protein / 11S protein / acid-precipitating protein
  • 7S protein and 11S protein are both main storage proteins stored in the protein body of soybean.
  • “7S protein” is also called ⁇ -conglycinin and is generally a glycoprotein composed of three types of subunits ( ⁇ ′, ⁇ , ⁇ ), but any subunit may be missing. These subunits are randomly combined to form a trimer. The isoelectric point is around pH 4.8 and the molecular weight is about 170,000.
  • “11S protein” is also called glycinin, and acidic subunit (AS) and basic subunit (BS) are linked by disulfide bond to form a 12-mer consisting of 6 molecules. About 360,000. Hereinafter, they may be abbreviated as “7S” and “11S”, respectively.
  • Both 7S and 11S are acid-precipitated soy proteins.
  • the “acid-precipitating protein” as used herein has a property of insolubilizing and precipitating acid by adjusting the pH of a solution such as defatted soymilk in the vicinity of the isoelectric point (pH 4 to 6) among proteins contained in soybean. It refers to protein. Therefore, for example, the protein contained in the separated soy protein corresponds to this, and the protein in whey that does not undergo acid precipitation during the production of the separated soy protein is not included.
  • SDS-PAGE SDS polyacrylamide gel electrophoresis
  • MSP Major Soy Protein
  • Lipophilic proteins are a group of minor acid-precipitating proteins other than 7S and 11S among soybean acid-precipitating proteins, and are accompanied by a large amount of polar lipids such as lecithin and glycolipid. Mainly the soybean membrane protein contained in the membrane component and the protein with high affinity to the membrane component are dispersed in a colloidal form and become acidic and insoluble.
  • the lipophilic protein may be simply abbreviated as “LP”.
  • This LP mainly includes proteins showing 34 kDa, 24 kDa, 18 kDa and 17 kDa in estimated molecular weights by SDS-PAGE, lipoxygenase, ⁇ -conglycinin and many other miscellaneous proteins.
  • This LP has the property that SDS-PAGE is harder to stain than 7S and 11S.
  • Embodiment (Powdered soybean material)
  • the powdery soy material of the present invention comprises soy protein and soy dietary fiber, the protein content is 35 to 85% by weight per non-fat solid content, and the LCI value of the protein is 60 or more.
  • the soy material is mainly composed of soy protein and soy dietary fiber in which LP different from 7S and 11S is particularly concentrated among acid-precipitating proteins. This will be described in more detail.
  • the protein content in the powdered soybean material of the present invention is 35 to 85% by weight per non-fat solid content, more preferably 42 to 68% by weight, and 45 to 65% by weight. % Is more preferable.
  • the “non-fat solid content” refers to a solid content obtained by removing neutral lipids extracted with ether from the total solid content. If the protein content in the soybean material is too small, the dietary fiber content will be relatively increased, the texture will be rough and the texture will increase, and the emulsification stability will tend to decrease. This is not preferable because the amount of lipid increases and the flavor tends to deteriorate.
  • the protein content is calculated by multiplying the nitrogen amount determined by the Kjeldahl method by the “nitrogen-protein conversion factor” (6.25).
  • the composition of the soy protein contained in the powdered soy material of the present invention is at least LP more concentrated than the composition of the soy protein contained in the soy material such as ordinary separated soy protein, concentrated soy protein, defatted soy, and okara. It is important that Since LP is a mixture of various proteins other than 7S and 11S among acid-precipitating proteins, it is difficult to specify all the proteins. In addition, since soybean materials such as separated soybean protein are generally heat-sterilized in the final productization process, 7S and 11S are heat-denatured together with LP. For this reason, it is difficult to measure LP content by separating LP from 7S and 11S from commercialized separated soybean protein and the like. In addition, SDS-PAGE, which is a general method for measuring protein composition, has the property that LP is less susceptible to CBB staining than 7S and 11S, and it is difficult to accurately measure the degree of staining.
  • an index called “LCI value” is used as an estimated value of LP content.
  • the LCI value is calculated by the following LCI value calculation method in which the main protein in each of the 7S, 11S, and LP proteins is simply selected, their staining ratio is obtained, and the LP content is estimated from these ratios. Is. Note that this method can be widely applied not only to soy materials such as general separated soy protein and concentrated soy protein, but also to various fractions such as soy 7S protein, soy 11S protein, and SPI with high LP.
  • LCI value- As main proteins in each protein, 7S selects ⁇ subunit and ⁇ ′ subunit ( ⁇ + ⁇ ′), 11S selects acidic subunit (AS), LP selects 34 kDa protein and lipoxygenase (P34 + Lx), and SDS Determine the staining ratio of each protein selected by PAGE (see FIG. 1). SDS-PAGE shall be performed under the conditions shown in Table 1.
  • B The estimated LP content (Lipophilic Proteins Content Index, hereinafter abbreviated as “LCI”) is calculated according to the equation (1).
  • the LCI value of the protein contained in the powdered soybean material of the present invention calculated by the above method is 60 or more.
  • the LCI value is preferably 62 or more, more preferably 65 or more, and further preferably 70 or more.
  • the LCI value is less than 60. Therefore, when the LCI value of the soybean material is less than 60, the quality does not change from that of a normal soybean material.
  • the LCI value of the isolated soybean protein obtained by the conventional production method is only about 35-50.
  • the LP content is about 20 to 30% by weight in the dry solid content, and the LCI value is only about 50. Since the soybean material having an LCI value of 60 or more is concentrated in LP, the composition of 7S protein and 11S protein, which are other acid-precipitated soybean proteins, is compared with that of ordinary soybean materials. It is relatively low. Therefore, it is not particularly necessary to limit the composition of the 7S protein and the 11S protein, but more specifically, these compositions are as follows.
  • the MSP content per protein in the powdered soybean material of the present invention increases as the proportion of LP decreases, that is, the LCI value decreases. If the soybean material contains a lot of MSP, it is not preferable because it approximates the composition of a normal concentrated soy protein and exhibits features such as stickiness and hard texture. Therefore, it is preferable that it is not contained as much as possible.
  • the MSP content is inversely correlated with the LCI value and is not particularly limited. However, in more detail, it is preferably 40% by weight or less per soy protein, more preferably 30% by weight or less, and 20% by weight or less. Is more preferable. In addition, when measuring about MSP content, it shall measure using SDS-PAGE by the following methods.
  • -MSP content calculation method Sodium sulfate is dissolved in the extract extracted from defatted soybean with water to a concentration of 1M, sodium sulfite as a reducing agent is added to a concentration of 10 mM, pH is adjusted to 4.5 with hydrochloric acid or sulfuric acid, and insoluble matter is added. And the fraction obtained by collecting the precipitate produced by dialysis to bring the ionic strength to 0.03 or less is referred to as “standard purified MSP”. The protein content of this standard purified MSP was determined by the Kjeldahl method, 5-15 ⁇ g of protein mass was applied to each well of the SDS-PAGE gel, electrophoresed, and then CBB stained (see FIG. 1).
  • the degree of staining is counted.
  • a calibration curve is created based on the relationship between the amount of protein applied and the degree of staining.
  • a measurement sample is applied and spread on the same slab gel. Since LP and other substances are present in addition to MSP in the measurement sample, only the degree of staining of the MSP band ( ⁇ subunit, ⁇ ′ subunit, ⁇ subunit, acidic subunit, basic subunit) is determined by densitometry. From the degree of staining, the content of MSP contained in the measurement sample is measured from the MSP calibration curve.
  • the powdery soybean material of the present invention must contain soybean dietary fiber. That is, LP and soybean dietary fiber coexist, and the combination of these exhibits a unique effect as a soybean material.
  • the lower limit of the content of soybean dietary fiber in the soybean material is usually 15% by weight or more, preferably 20% by weight or more, more preferably 25% by weight or more, and further preferably 30% by weight or more with respect to protein.
  • the upper limit of the content of soybean dietary fiber in the soybean material is usually 130% by weight or less, preferably 100% by weight or less, more preferably 95% by weight or less, and still more preferably 90% by weight or less based on protein.
  • the dietary fiber content shall be in accordance with the “Fiveth Amendment Japanese Food Standard Ingredients Table” (Ministry of Education, Culture, Sports, Science and Technology, 2005), and the dietary fiber content is measured by the enzyme-weight method (Prosky modified method). If soy dietary fiber is not contained or is too little, and the protein content in the soy material exceeds 85% by weight, the LP occupies most of the composition, and the flavor such as LP-specific deteriorated odor becomes remarkable. Physical properties such as dispersibility and shape retention tend to decrease. On the other hand, if the soybean fiber is too much and the protein content is less than 35% by weight, the texture is likely to be rough and crunchy, and the physical properties such as emulsion stability tend to decrease.
  • the powdery soy material does not substantially contain whey component sugar or acid-soluble soy protein (protein that does not undergo acid precipitation at pH 4 to 6). That is, it is preferable that the whey component is extracted and removed from the raw material during the production of the soybean material. If the soy material contains a large amount of whey component, the content of dietary fiber is relatively lowered, and the emulsifiability and water retention are deteriorated.
  • the raw material of the soybean material either full-fat soybean or defatted soybean can be used.
  • the defatted soybean it is preferable to use the defatted soybean.
  • soybeans that are partially or entirely deficient in 7S and / or 11S by breeding or genetic manipulation can be used as raw materials.
  • a method for preparing the soybean material of the present invention using these raw materials a method in which LP is selectively insolubilized and fractionated with 7S and 11S to concentrate LP can be used.
  • a method described in Patent Document 1 WO 2006/129647
  • soybean is heat-denatured or alcohol-denatured to obtain a processed soybean having a PDI of 40 or more and less than 80, which is pulverized if necessary, extracted with water, and its insoluble fraction is recovered and dried into powder.
  • soybean is heat-denatured or alcohol-denatured to obtain a processed soybean having a PDI of 40 or more and less than 80, which is pulverized if necessary, extracted with water, and its insoluble fraction is recovered and dried into powder.
  • Patent Document 1 The method of Patent Document 1 will be described more specifically.
  • PDI Protein Dispersibility Index
  • a dry heating method using a roasting device, a hot air heating device, a microwave heating device, etc., or a wet heating method using a humidification heating device, a steaming device, a steam heating device, etc. can be employed without any particular limitation. However, it is better to avoid heating because the protein is extracted when the water is soaked in soybeans. As an example, a method in which soybean is enclosed in a sealed tank and the inside of the jacket covering the outside of the sealed tank is heated so that the product temperature is about 70 to 95 ° C. in an atmosphere with a relative humidity of 90% or more can be employed.
  • the heating temperature and time conditions in this case are not particularly limited as long as LP insolubilization is selective, but the temperature is usually set to 60 to 95 ° C., and the time is 1 minute to 10 hours. It is appropriate to perform between.
  • a heating device such as a heating device using superheated steam, an extruder, or a jet cooker may be used.
  • the equivalent weight or less preferably 2 to 100 parts by weight, more preferably 8 parts by weight with respect to raw soybean containing protein and okara components.
  • a method of adding and impregnating ⁇ 20 parts by weight, more preferably 10 to 15 parts by weight of a polar alcohol solution is preferred.
  • This method is a method for washing non-protein components such as saccharides of soybeans by immersing soybeans in many times the amount of alcohol and suspending them, as in the conventional method of producing concentrated soy protein by alcohol washing. The idea is completely different, and an equal weight or less polar solvent solution is added to and impregnated with soybean.
  • the state of the mixed soybean is typically a wet powder state.
  • the addition amount of the polar solvent is lower than 2% by weight with respect to soybean, the selective water insolubilization of LP becomes insufficient, so that the effect of suppressing the extraction of LP during water extraction tends to be insufficient.
  • the weight is greater than the equal weight, non-selective water insolubilization in which 7S and 11S are insolubilized with LP tends to occur, and the extraction of 7S and 11S becomes insufficient.
  • a polar solvent suitable for promoting selective water insolubilization of LP a polar alcohol solution (methanol, ethanol, propanol, isopropanol, etc.) can be used.
  • an aqueous ethanol solution frequently used in the food industry.
  • the water may be pure water, or an acid aqueous solution (hydrochloric acid aqueous solution, carbonic acid aqueous solution, citric acid aqueous solution, etc.), alkaline aqueous solution (sodium hydroxide solution, sodium bicarbonate, etc.), etc. may be used.
  • the concentration of the polar solvent solution is preferably 5 to 100%, more preferably 50 to 80%.
  • the method for adding the polar solvent solution can be carried out by, for example, a method of spraying the powder by spraying or a method of dropping, but is not particularly limited.
  • a mixing method after adding the polar solvent solution for example, a stirrer such as a kneader or a high-speed stirrer can be used.
  • a heating treatment in combination it is more preferable to use a heating treatment in combination.
  • the heating temperature is preferably 30 to 95 ° C, more preferably 40 to 90 ° C, as the product temperature of soybean.
  • the heating time is preferably 5 to 100 minutes, more preferably 10 to 60 minutes.
  • the order of these steps is not particularly limited.
  • the polar solvent is added and mixed and then the heating treatment is performed, or the polar solvent is added and mixed.
  • Heat treatment is preferably performed.
  • the process for removing the polar solvent after the treatment becomes extremely easy as compared with the conventional alcohol washing method, etc., which is advantageous in establishing an efficient manufacturing process.
  • Most of the polar solvent remaining in the processed soybean can be volatilized by heating treatment, and this can be directly applied to the extraction process.
  • the product temperature is 40-60. If treated for 10 to 60 minutes at °C and reduced pressure (about -10mmHg), it can be completely volatilized and returned to the weight of soybean before addition.
  • the volatilized polar solvent is advantageous in terms of the manufacturing process because it can be reused if it is recovered by distillation.
  • the processed soybeans obtained as described above are pulverized if necessary, added with 2 to 10 times the amount of water, stirred and suspended to extract water, and 7S and 11S are positively added to the water-soluble fraction side. Extracted into This water-soluble fraction is removed by solid-liquid separation such as centrifugation or filtration, and the remaining insoluble fraction is recovered.
  • This insoluble fraction is mainly composed of insolubilized LP and soybean dietary fiber, and the LP is relatively concentrated, and the LCI value is 60 or more.
  • the pH at the time of water extraction is usually neutral to alkaline (pH 7 to 12), but if you want to increase the protein content of the powdered soybean material as much as possible, the pH of the suspension should be acidic at the time of water extraction.
  • the protein dissolved in the water-soluble fraction side can be transferred to the insoluble fraction side.
  • 7S and 11S move to the insoluble fraction side as the pH approaches the isoelectric point of soy protein, and the LCI value decreases. Therefore, care must be taken so that the LCI value does not become less than 60, depending on the conditions.
  • Even when the pH is adjusted to the acidic side it is usually appropriate to adjust the pH to 5 or more, preferably 5.5 or more.
  • the insoluble fraction obtained as described above is finally dried and powdered to obtain the powdered soybean material of the present invention.
  • dry powdering can use well-known drying methods, such as a spray dryer and freeze drying.
  • dietary fiber can be made water-soluble by pressure heat treatment, some insoluble dietary fiber can be removed during the manufacturing process, or heat sterilization treatment Can be done.
  • a fine pulverization process or a granulation process can be performed.
  • soybeans in which a part or all of 7S and / or 11S has been deleted by breeding or genetic manipulation are used as a raw material. Since these soybeans have a relatively high LP content compared to general soybeans, depending on the variety, the soybeans can be crushed into soy flour without using a fractionation method, or acid washing or alcohol By simply removing whey by washing to obtain concentrated soy protein or preparing okara by removing soy milk, it can be prepared into a powdered soy material that meets the specific requirements of the present invention. In this case as well, it is preferable that LP is insolubilized and coexisting with dietary fiber.
  • the above-mentioned deficient soybean is processed into soybean powder as a raw material to obtain the powdered soybean material of the present invention.
  • the raw material is subjected to dry heat treatment or wet heat treatment, and the PDI of soybean is preferably reduced to 40 or more and less than 80.
  • the powdery soybean material of the present invention obtained as described above is characterized by being mainly composed of LP and soybean dietary fiber.
  • the soybean material can be used in various edible compositions, and various physical properties of the edible composition can be improved.
  • the physical properties are general properties of dispersibility, shape retention, water retention, emulsification stability, oxidation stability, viscosity, plasticity, texture, etc. of the edible composition.
  • the soybean material when used in an edible composition, it has a smooth texture, less stickiness, light mouthfeel, and throat compared to existing soybean materials such as conventional separated soybean protein and concentrated soybean protein.
  • LP is the main component of the protein composition, it is possible to prepare an edible composition that enhances the physiological function of the protein and an edible composition that makes better use of its properties by combining with soy dietary fiber.
  • 7S protein is highly viscous depending on processing and may cause stickiness, which may cause an unpleasant texture.
  • 11S protein may cause roughness due to processing, particularly when heated in the presence of a salt such as calcium ion or magnesium ion, because it reacts and aggregates significantly.
  • the cohesiveness by an acid is also high, and when it uses for an acidic edible composition, it may affect tastes, such as roughness and astringency.
  • soy protein gels mainly composed of 7S and 11S are hard and elastic, and depending on processing conditions, they may not dissolve in the mouth forever, resulting in poor gel quality in the throat. In this way, processed foods may have undesired physical properties due to processing reasons, which may be related to protein composition.
  • soybean dietary fiber can impart stable shape retention and water retention. Soybean dietary fiber alone is not preferable because it has the texture of its own texture and low emulsifying power. By coexisting with LP, the texture of soybean dietary fiber is relaxed, a smooth texture is realized, and emulsifying power is also imparted.
  • the edible composition to which the powdered soybean material of the present invention is used include, for example, a powdered composition, an emulsified composition, a baked or fried confectionery, a kneaded product, a textured composition, a semisolid form
  • a powdered composition an emulsified composition
  • a baked or fried confectionery a kneaded product
  • a textured composition a semisolid form
  • a gel composition or a fried food material a fried food material (batter)
  • the powdery soybean material of the present invention is rich in protein and dietary fiber, it can be used together as a physiologically functional material.
  • functional foods aimed at demonstrating nutritional functions such as reducing blood cholesterol, preventing diabetic nephropathy, suppressing blood sugar rise, improving intestinal environment, etc.
  • Many fibers are contained, and physical properties of the edible composition are likely to change. Therefore, when the above-described physical property improving effect is exerted, it works more effectively, so it is preferable
  • Powdered compositions include powdered beverages, powdered soups, powdered sauces, powdered flavorings, powdered pickles, cakes, breads, confectionery, noodles, and fried food clothes.
  • powdered compositions include flour mixes such as ingredients and side dishes (okonomiyaki).
  • the powdery soybean material of the present invention is used in a powdery composition, if it is dispersed in water, it is less likely to become lumpy than separated soybean protein, and the dispersibility is very good.
  • a soup or a flavoring agent having a high oil content it is excellent in emulsification stability, so that oil stains are less likely to occur.
  • the oxidative deterioration of the oil in the flavor can be suppressed, contributing to oxidation stability.
  • a crispy feeling can be provided to fried foods and the oil stain after frying can be suppressed.
  • Emulsified compositions examples include O / W emulsified compositions or W / O emulsified compositions such as mayonnaise, whipped cream, custard cream, flour paste, cheeses and sauces. .
  • W / O emulsified compositions such as mayonnaise, whipped cream, custard cream, flour paste, cheeses and sauces.
  • the powdered soybean material of the present invention is used in an emulsion composition that contains a relatively large amount of oil, it is superior in emulsifying properties, shape retention, viscosity, etc., compared to adding normal concentrated soybean protein or okara. It provides a texture-improving effect that is smooth and non-textured.
  • the emulsified composition softens and sags even when cooked when used as a filling or topping for cooking pans, etc., and does not easily cause oil separation, and has excellent heat-resistant shape retention. Is. Further, when the emulsion composition is applied as a dressing to a vegetable salad or the like, the emulsion can be prevented from dripping due to an increase in moisture. Moreover, since the powdery soybean material of the present invention itself has little stickiness and roughness, it can also be used as a viscosity modifier for an emulsified composition.
  • High content and low calorie product Non-limitatively containing 20% by weight or more, preferably 25% by weight or more of dietary fiber and soy protein having an LCI value of 60 or more, containing 10% by weight or more, preferably 15% by weight or more, and having an energy of 100 g Per kcal or less, preferably 400 kcal or less.
  • the dough does not harden and has an appropriate plasticity, so that the preparation of the dough is extremely easy, and an appropriate hardness after baking. It can give a light and meltable mouthfeel.
  • the shape retention is high, it is possible to prevent the edge portion of the pound cake after the baking from collapsing, and the peeling from the baking mold after the baking is improved.
  • about fried confectionery it is possible to suppress the amount of oil absorption or to give a crisp feeling.
  • the baked confectionery when preparing a high dietary fiber, high protein, low calorie snack confectionery, for example, it can be prepared as follows.
  • the powdered soybean material of the present invention is combined with a combination of dietary fiber materials such as wheat bran, potato fiber, indigestible dextrin, polydextrose, inulin, etc., mixed with other ingredients, kneaded with water, molded, Bake.
  • a baked confectionery having a good taste, in which the powdery soybean material and the dietary fiber material occupy 50 to 95% by weight in the dry solid content.
  • LP has a part where protein is associated with lipid, and is less susceptible to low molecular weight by protease compared to storage proteins such as 7S and 11S. It can also be used as a dietary supplement with reduced calories.
  • Kneaded product examples include sausage, hamburger, tsukune, ganmodoki, fried chicken, kamaboko, chikuwa and the like.
  • the powdery soybean material of the present invention When the powdery soybean material of the present invention is added to the kneaded product as a powder, it exhibits good dispersibility in the dough, so that formation of lumps is prevented. Further, the kneaded product can be given a texture that is not rubbery and has a good crispness and throat passage. Further, even if a relatively severe heat shock such as retort heating is applied to these kneaded products, the physical properties hardly change and the retort resistance is strong.
  • Semi-solid or gel composition examples include pudding, jelly, concentrated liquid food, yogurt-like fermented soybean food, and the like.
  • a semi-solid or gel-like composition unlike a conventional strong gel with flexibility such as 7S or 11S, a soft gel-like or highly viscous semi-solid It can provide a creamy and smooth texture.
  • a good flavor can be realized with almost no blue odor derived from soybeans.
  • the powdered soybean material of the present invention may be used alone, or a soymilk or soybean powder suspension is used in combination, and the desired physical properties are appropriately adjusted. It is also possible.
  • the powdery soybean material of the present invention is a physiologically functional material that is difficult to impair the flavor and physical properties that the edible composition originally has, in addition to being able to impart physical properties improving effects to various edible compositions as described above. Can also be used.
  • the soy material is rich in protein and dietary fiber, and also rich in phospholipids as polar lipids, so it can be used to nutritionally strengthen these components.
  • LP which is a main protein in the soybean material, is a component in which the effect of reducing cholesterol and the effect of preventing diabetic nephropathy are recognized among soybean proteins.
  • soy dietary fiber is said to have an effect of easing blood sugar rise, improving intestinal flora, and antioxidant effect, and supplementing dietary fiber, which is said to be low in consumption by modern people It can also be used for anti-aging purposes. Therefore, it is considered to be effective for suppressing blood sugar elevation, protecting kidneys, improving lipid metabolism, intestinal regulation, and dieting, and is extremely useful as a food material for preventing lifestyle-related diseases.
  • the powdered soybean material is not only used for improving the physical properties of the edible composition, but also used as a protein reinforcing material or a dietary fiber reinforcing material that does not obstruct the original physical properties of the edible composition.
  • An edible composition with enhanced nutritional value can be produced.
  • by strengthening these components it is possible to produce a functional food that exhibits physiological functions such as an increase in blood sugar, a kidney protecting action, a lipid metabolism improving action, and an intestinal regulating action.
  • the type of functional food is not particularly limited, and various edible compositions of the above (1-1) to (1-5) are exemplified.
  • it is preferable that at least these components are contained in the composition in an amount of 3% by weight or more, and more preferably 5% by weight or more.
  • LP is considered to be a cause of soybean off-flavor, and it has not always been said that the product with high purity has a good flavor.
  • LP is contained in combination with soybean dietary fiber, so that it is surprisingly difficult to feel off-flavor due to LP, and the flavor is good.
  • the soy dietary fiber originally has a poor texture when used alone, but the coexistence with LP makes the texture surprisingly smooth and difficult to feel. Therefore, when the soybean material is added to the above functional food, each defect of LP or soybean dietary fiber is less likely to be affected, and it is extremely versatile as a functional food material that does not easily impair physical properties and flavor. is there.
  • soybean materials A to E used in Examples and Comparative Examples, those prepared as follows or commercially available products were used.
  • the obtained defatted soybean slurry (pH 6.4) was separated into a soluble fraction and an insoluble fraction by centrifugation. This insoluble fraction was collected, water three times the weight of the insoluble fraction was added, heat sterilized, and then dried and powdered with a spray dryer to obtain soybean material A.
  • Soybean material B Commercially available concentrated soy protein “Arcon S” (manufactured by ADM) was used.
  • the obtained defatted soybean slurry (pH 6.0) was separated into a soluble fraction and an insoluble fraction by centrifugation. This insoluble fraction was collected, water three times the weight of the insoluble fraction was added, heat sterilized, and then dried and powdered with a spray dryer to obtain soybean material C.
  • Soybean material D A commercially available dry okara powder “PF-20” (manufactured by Tensei Co., Ltd.) was used.
  • soybean materials A to E the protein content (per fat-free solid content), dietary fiber content (per fat-free solid content), LCI value, MSP content (per protein) were measured, and the analysis results are shown in Table 2. It was shown to. Each measurement method was performed by the method described above.
  • Example 1 Powdered beverage- A powdered beverage was prepared as an example of the powdery composition of the present invention.
  • the soybean material A of the present invention (protein content 45%, LCI value 70) was mixed in milk and stirred with a spoon. Dispersion was good, and no solid insoluble powder lump such as ma powder was found. Despite containing okara, it had a smooth texture with little roughness.
  • soybean material B protein content 70%, LCI value 48
  • Dispersion was poor, and hard mako was produced.
  • soybean material D protein content 25%, LCI value 54
  • Dispersion was good, but most of it sank, did not dissolve and felt very rough.
  • Example 2 a powdery taste agent was prepared. Emulsification obtained by adding 6 parts of soybean material C (protein content 60%, LCI value 66) of the present invention to 100 parts of a ramen flavoring agent having a solid content of 20% and containing 50% of oil, and mixing by heating. The product was spray-dried to prepare a powdery taste agent. The powder obtained had little oil stain and high emulsification stability, and when added to water, it was moderately dispersed and the taste felt natural.
  • soybean material C protein content 60%, LCI value 66
  • Example 3 Application to fried food clothing-
  • a fried food apparel was prepared. 10 parts of soybean material A (protein content 45%, LCI value 70) of the present invention and 90 parts of soft flour were mixed to prepare a fried food apparel. This was dissolved in water to give a viscosity of about 1600 mPa ⁇ s with oil, and the texture was confirmed as fried. A moderate crispness was felt.
  • Example 4 - Mayonnaise- Mayonnaise was prepared as an example of the emulsified composition of the present invention. 4 parts of soybean material C (protein content 60%, LCI value 66) of the present invention, 41 parts of water, 50 parts of soybean oil and 5 parts of vinegar are added to a food cutter and stirred for 5 minutes to O / W emulsify, and mayonnaise Prepared. The mayonnaise was smooth and melted comfortably in the mouth. Moreover, it had shape retention property, and even when heated, the oil was not separated and the emulsion stability was excellent.
  • soybean material C protein content 60%, LCI value 66
  • soybean material D protein content 25%, LCI value 54
  • Table 4 shows the state of emulsification of each mayonnaise prepared in Example 4 and Comparative Examples 7 and 8.
  • Example 5 -Bound cake- As a first example of the baked confectionery of the present invention, a bound cake was prepared. 20 parts of soy material A (protein content 45%, LCI value 70) of the present invention was mixed with 20 parts of margarine, 20 parts of sugar and 24 parts of egg white, and baked to obtain a bound cake.
  • soy material A protein content 45%, LCI value 70
  • soybean material D protein content 25%, LCI value 54
  • 20 parts of soybean material D protein content 25%, LCI value 54
  • Example 6 -Senbei- As a second example of the baked confectionery of the present invention, rice crackers were prepared. 20 parts of soybean material A (protein content 45%, LCI value 70) of the present invention, 20 parts of margarine and 15 parts of sugar were mixed, and 15 parts of water was added and kneaded to prepare a dough. This was sandwiched between hot iron plates and heated to prepare a rice cracker.
  • soybean material A protein content 45%, LCI value 70
  • a rice cracker was prepared by the same composition and preparation method as in Example 6 using soybean material E (protein content 40%, LCI value 52) instead of soybean material A.
  • soybean material D protein content 25%, LCI value 54 was used to prepare rice crackers by the same composition and preparation method as in Example 6.
  • Example 7 -Retort sausage- Retort sausage was prepared as an example of the kneaded product of the present invention.
  • 100 parts of soybean material A (protein content 45%, LCI value 70) of the present invention 400 parts of water and 100 parts of pork fat are mixed in a kneader to make a sufficiently uniform dough, then 30 parts of starch and spices, etc. 20 parts of a flavoring agent containing was added and mixed, filled into a casing and heated with retort to obtain a retort sausage.
  • the edible composition exhibited a good sausage-like smooth throat texture.
  • Example 8 -Use for yogurt-like fermented soybean food-
  • a yogurt-like fermented soybean food was prepared. As shown in Table 8, water was heated to 60 ° C., and while stirring, soy material A (protein content 45%, LCI value 70) and raw materials other than lactic acid bacteria were added. After dissolution, the pressure was increased to 15 MPa using a high-pressure homogenizer. And homogenized. Thereafter, it was heated to 90 ° C. and then cooled to 43 ° C. Next, lactic acid bacteria are added, filled into a cup, sealed with a lid, fermented for about 5 hours in a thermostatic bath at 43 ° C. until the pH drops to 4.5, then cooled to below 10 ° C., and yogurt-like Of soy fermented food.
  • soy material A protein content 45%, LCI value 70
  • the pressure was increased to 15 MPa using a high-pressure homogenizer. And homogenized. Thereafter, it was heated to 90 ° C. and then cooled
  • Example 8 contains soybean dietary fiber as compared with Comparative Example 15, there is almost no blue odor derived from soybean, and the protein components of 7S and 11S are almost lost. Because of this, it was a yogurt-like physical property with a creamy and smooth texture.
  • Example 9 -Snack-style confectionery- A snack-like confectionery was prepared as a third example of the baked confectionery of the present invention. 10 parts of granulated sugar was mixed with 5 parts of palm oil, 25 parts of soybean material A and 15 parts of wheat bran were added and mixed, and 70 parts of water was added and kneaded to obtain a baked confectionery dough. When the dough was thinly stretched and molded and baked at 160-170 ° C. for 30 minutes, a crisp snack-like baked confection was obtained.
  • the baked confectionery was a high protein, high dietary fiber, low calorie baked confectionery that contained 21% protein derived from soybean material A, 31% total dietary fiber, and had an energy of 400 kcal per 100 g.
  • Example 10 a nutrition bar was prepared. 50 parts of margarine, 30 parts of granulated sugar, 0.2 part of salt, and 27 parts of ganache are sequentially mixed, then 63 parts of soybean material A, 33 parts of indigestible dextrin, 1.5 parts of cocoa powder, and modified starch 24 In addition, 45 parts of raisins, 12 parts of orange peel, 12 parts of dried pine, and 12 parts of sliced almonds were added, and 45 parts of whole eggs were finally added and mixed well to prepare a dough. The obtained dough was formed into a shape of 10 cm ⁇ 20 cm ⁇ 70 cm and baked at 170 ° C. for 22 minutes to obtain a nutrition bar. This baked confectionery was 76 kcal per one, and contained about 3% protein and about 4% dietary fiber.
  • soy material such as separated soybean protein and concentrated soybean protein has been difficult so far. It can give quick dispersibility to the kneaded dough with less solution and moisture, and can also give water retention, shape retention, emulsification stability and smooth texture without any sense of incongruity it can.
  • the powdered soybean material of the present invention is less susceptible to adverse effects on the physical property improvement effect of the above edible composition even under acidic conditions and in the presence of divalent metal ions that easily interact with proteins such as magnesium and calcium.
  • the powdered soybean material of the present invention itself is rich in LP and dietary fiber, it has excellent physiological functions, cholesterol reduction action, bile acid excretion action, nephropathy prevention action, diet action, intestinal action by dietary fiber, It can be used in various edible compositions for the purpose of improving the intestinal flora.

Abstract

Provided is a powdery soybean material which enables the utilization of the characteristic nutritional functions or properties, for example, cholesterol-lowering effect and nephropathy-preventing effect, of lipophilic proteins, has good processability as a food material and, therefore, can impart nutritional functions to an edible composition without impairing the flavor or texture of the same. Also provided are a powdery soybean material which has good processability not exhibited by the conventional soybean materials without being restricted to the nutritional properties of lipophilic proteins, and an edible composition with the use of the characteristic physical properties of said material. The powdery soybean material is characterized by comprising soybean proteins and soybean dietary fibers, having a protein content of 35-85 weight% relative to the weight of non-fat solid matters, and showing an LCI value of the proteins equal to or greater than 60.

Description

粉末状大豆素材及びこれを利用した食用組成物Powdered soybean material and edible composition using the same
 本発明は粉末状大豆素材及びこれを利用した食用組成物に関する。詳しくは、大豆から取り出した特定の画分を用いた粉末状大豆素材および該大豆素材特有の機能を利用した食用組成物に関する。 The present invention relates to a powdery soybean material and an edible composition using the same. In detail, it is related with the edible composition using the powdery soybean raw material using the specific fraction taken out from soybean, and the function peculiar to this soybean raw material.
 大豆蛋白質は、特有のゲル化力を発揮する性質から、食用組成物の物性改善に幅広く利用されており、また蛋白質の補給源として栄養価の高い健康食品素材としての利用も増大している。
 大豆の貯蔵蛋白質は、pH4.5付近で沈澱し、比較的簡単に貯蔵蛋白質以外の酸可溶性成分が主体の大豆ホエー蛋白質画分と貯蔵蛋白質が主体の酸沈殿性蛋白質画分とに分けることができる。この酸沈殿性蛋白質画分を回収したものが分離大豆蛋白であり、ゲル形成能力に富み、栄養的にもアミノ酸スコアが高く、現在広く食品工業分野で利用されている。
Soy protein is widely used for improving the physical properties of edible compositions because of its unique gelling power, and is also increasingly used as a health food material with high nutritional value as a protein supply source.
Soybean storage protein precipitates around pH 4.5 and can be divided into a soybean whey protein fraction mainly composed of acid-soluble components other than the storage protein and an acid-precipitated protein fraction mainly composed of storage protein. it can. A product obtained by collecting this acid-precipitating protein fraction is a separated soy protein, which has a high gel forming ability and a high amino acid score nutritionally, and is currently widely used in the food industry.
 大豆蛋白質を構成する蛋白質は、超遠心分析による沈降係数から、2S,7S,11S,15Sの各蛋白質に分類される。このうち、7S蛋白質と11S蛋白質は大豆の酸沈殿性蛋白質の主要な構成成分である。なお、免疫学的命名法にいうβ-コングリシニンは7S蛋白質に、グリシニンは11S蛋白質に実質的に相当するものである。そしてこれらの主要な蛋白質は、粘性、凝固性、界面活性などの物性や栄養生理機能において異なる性質を有する。 Proteins constituting soy protein are classified into 2S, 7S, 11S, and 15S proteins based on the sedimentation coefficient obtained by ultracentrifugation analysis. Of these, 7S protein and 11S protein are the main constituents of soy acid-precipitating protein. In the immunological nomenclature, β-conglycinin substantially corresponds to the 7S protein, and glycinin substantially corresponds to the 11S protein. These major proteins have different properties in physical properties such as viscosity, coagulability and surface activity, and nutritional physiological functions.
 例えば7S蛋白質は栄養学的には血中の中性脂肪を低下させることが報告されている(非特許文献1)。そのゲルは弾力のあるゲル質を示し、液の粘性が高い。また、11S蛋白質は、ゲル化力が高く、豆腐ゲルの硬さ・食感を支配していると言われている。このように7S蛋白質と11S蛋白質はいずれも比較的強いゲル形成能を示すことが特徴であるが、その物性は加工条件によって異なる。例えば水分量が多い系での加熱では、7S蛋白質の特性から影響する食感の変化としてはネチャツキを感じたり、11S蛋白質の酸やCa,Mgイオンに対する加熱時の挙動の結果として強い凝集力が生まれることによる味の変化やザラツキが感じられるようになり、必ずしも食用組成物として好ましい状態にならない場合もある。 For example, 7S protein has been reported to nutritionally reduce neutral fat in blood (Non-patent Document 1). The gel shows an elastic gel and the liquid is highly viscous. 11S protein has a high gelling power and is said to control the hardness and texture of tofu gel. Thus, both 7S protein and 11S protein are characterized by relatively strong gel-forming ability, but their physical properties differ depending on processing conditions. For example, when heating in a system with a large amount of water, the texture changes due to the characteristics of the 7S protein, and the texture changes, and the cohesive strength is strong as a result of the heating behavior of the 11S protein to acids, Ca and Mg ions. Changes in taste and roughness due to being born can be felt, and it may not always be preferable as an edible composition.
 一方、近年大豆中の酸沈殿性蛋白質には7S蛋白質や11S蛋白質以外にも、細胞膜をはじめプロテインボディーやオイルボディー等の膜を構成する極性脂質との親和力の高い雑多な蛋白質の集合体である、「脂質親和性蛋白質」の存在が明らかになっている(非特許文献2)。この脂質親和性蛋白質は、コレステロール低減効果(特許文献1)や、腎症予防効果を有することが認められており(特許文献2)、特異な栄養機能が明らかにされつつある。
 一方で脂質親和性蛋白質の物性については詳しく知られておらず、食品素材としての加工適性に関して、特許文献3では、脂質親和性蛋白質の一つであるオレオシンとリン脂質との複合体を、乳化安定剤として利用する技術が開示されている。ただし、脂質親和性蛋白質はこのように複合体となって脂質を随伴するため酸化されやすく、オフフレーバーの原因とも言われている。そのため食品素材としては風味が悪く使いづらいと考えられており、栄養機能面の利点を実際の利用に活かすには未だほど遠い状態であるのが現状である。
On the other hand, in recent years, the acid-precipitating protein in soybeans is an aggregate of various proteins with high affinity with polar lipids constituting membranes such as cell membranes, protein bodies and oil bodies in addition to 7S protein and 11S protein. The existence of “lipophilic protein” has been revealed (Non-patent Document 2). This lipophilic protein is recognized to have a cholesterol-reducing effect (Patent Document 1) and a nephropathy-preventing effect (Patent Document 2), and a specific nutritional function is being clarified.
On the other hand, the physical properties of the lipophilic protein are not known in detail. Regarding the processability as a food material, Patent Document 3 emulsifies a complex of oleosin and phospholipid, which is one of lipophilic proteins. A technique for use as a stabilizer is disclosed. However, lipophilic proteins are complexed in this way and accompanied by lipids, so that they are easily oxidized and are said to cause off-flavour. For this reason, it is considered that the food material has a bad flavor and is difficult to use, and it is still far from being used for the practical use of the nutritional function.
WO2006/129647号公報WO2006 / 129647 WO2009/110504号公報WO2009 / 110504 gazette WO2002/26788号公報WO2002 / 26788
 上記実情に鑑み、本発明は、脂質親和性蛋白質が持つコレステロール低減効果や腎症予防効果などの特徴ある栄養機能特性を活かすことができ、かつ食品素材としての加工適性にも優れることにより、食用組成物の風味や食感を損ねることなく栄養機能を付加することのできる粉末状大豆素材を提供することを課題とする。また脂質親和性蛋白質の栄養機能特性に拘ることなく、従来の大豆素材にはなかった優れた加工適性を有する粉末状大豆素材を提供し、そして特徴ある物性を活かした食用組成物を提供することを課題とする。 In view of the above circumstances, the present invention is able to take advantage of characteristic nutritional functional properties such as cholesterol-reducing effects and nephropathy-preventing effects possessed by lipophilic proteins, and is excellent in processability as a food material. It is an object of the present invention to provide a powdered soybean material that can add a nutritional function without impairing the flavor and texture of the composition. In addition, the present invention provides a powdered soy material having superior processability, which is not found in conventional soy materials, and provides an edible composition that takes advantage of characteristic physical properties, regardless of the nutritional functional characteristics of lipophilic proteins. Is an issue.
 上記課題を解決すべく、本発明者らは、脂質親和性蛋白質を分画し、その物性や風味について詳しく調べた結果、単体の脂質親和性蛋白質はゲル形性力や凝集力が7S蛋白質や11S蛋白質よりも弱いことを見出した。そしてさらに脂質親和性蛋白質と大豆の他の成分との組合せについて検討を進めた結果、濃縮された脂質親和性蛋白質と大豆食物繊維が主体となった画分に着目した。そして、この画分を取り出しその物性を探求したところ、食感の滑らかさやネチャツキの低減、さらに乳化安定性と保形性等の種々の能力を合わせ持つ素材となり得ることを認め、脂質親和性蛋白質の特性を活かした利用が図れる可能性を見出し、上記課題を解決するに至った。 In order to solve the above-mentioned problems, the present inventors fractionated lipophilic proteins and investigated in detail their physical properties and flavors. As a result, the single lipophilic protein was found to have a 7S protein having a gel formability and a cohesive strength. It was found to be weaker than the 11S protein. As a result of further investigations on the combination of lipophilic protein and other components of soybean, attention was focused on the fraction mainly composed of concentrated lipophilic protein and soybean dietary fiber. And, when this fraction was taken out and the physical properties were explored, it was recognized that it could be a material having various capabilities such as smoothness of texture and reduction of stickiness, and emulsion stability and shape retention. As a result, the inventors have found the possibility of utilizing the characteristics of the above and have solved the above problems.
 すなわち本発明者らは、ゲル形成力や凝集力の少ない脂質親和性蛋白質と大豆食物繊維を主体としたものを粉末状大豆素材とすることを着想するに到り、かかる素材について様々な食用組成物の物性改良に使用が可能であることを見出した。
 なお、従来のオカラ等の大豆食物繊維が主体の素材では、保水性を有するものの、それ自体で結着してまとまる力が少ないために保形性が弱く、食感もざらつきを感じ、唾液を吸い取られるように感じる場合が多かった。これに対し、本発明は脂質親和性蛋白質と大豆食物繊維との組み合わせによって、適度な結着力による保形性および滑らかな食感と乳化性を合わせ持つ素材を見出したものである。
That is, the present inventors have come up with the idea that a powdery soy material is mainly composed of a lipophilic protein with little gel-forming ability and cohesive force and soy dietary fiber, and various edible compositions for such material. It was found that it can be used to improve the physical properties of materials.
In addition, conventional materials mainly composed of soy dietary fiber such as Okara have water retention, but their shape retention is weak because of their low binding and cohesiveness. In many cases, it felt like being sucked up. On the other hand, the present invention has found a material having both a shape retaining property with an appropriate binding force and a smooth texture and emulsifying property by combining a lipophilic protein and soybean dietary fiber.
 すなわち本発明は、
(1)大豆蛋白質及び大豆食物繊維を含有し、蛋白質含量が無脂固形分重量あたり35~85重量%であって、蛋白質のLCI値が60以上であることを特徴とする粉末状大豆素材、
(2)大豆食物繊維含量が蛋白質に対して15~130重量%である、前記(1)記載の粉末状大豆素材、
(3)前記(1)記載の粉末状大豆素材を使用した食用組成物、
(4)粉末状組成物、乳化組成物、焼きもしくは揚げ菓子、練り製品、又は半固形状ないしゲル状組成物である、前記(3)記載の食用組成物、
(5)食用組成物が、蛋白質又は食物繊維を強化したものである、前記(4)記載の食用組成物、
(6)大豆蛋白質及び大豆食物繊維を含有し、蛋白質含量が無脂固形分重量あたり35~85重量%であって、蛋白質のLCI値が60以上である粉末状大豆素材を食用組成物中に配合することを特徴とする食用組成物の物性改良方法、
(7)物性が分散性、保形性、保水性、乳化安定性、酸化安定性又は食感である前記(6)記載の食用組成物の物性改良方法、である。
That is, the present invention
(1) A powdered soy material comprising soy protein and soy dietary fiber, wherein the protein content is 35 to 85% by weight per non-fat solid content, and the LCI value of the protein is 60 or more,
(2) The powdered soybean material according to (1), wherein the dietary fiber content of soybean is 15 to 130% by weight based on protein,
(3) An edible composition using the powdered soybean material according to (1),
(4) The edible composition according to (3), which is a powdered composition, an emulsified composition, a baked or fried confectionery, a kneaded product, or a semi-solid or gel-like composition,
(5) The edible composition according to the above (4), wherein the edible composition is a protein or dietary fiber reinforced.
(6) A powdered soy material containing soy protein and soy dietary fiber, having a protein content of 35 to 85% by weight of the non-fat solid content and a protein LCI value of 60 or more is contained in the edible composition. A method for improving the physical properties of an edible composition, characterized by comprising:
(7) The method for improving physical properties of an edible composition according to the above (6), wherein the physical properties are dispersibility, shape retention, water retention, emulsion stability, oxidation stability or texture.
 本発明により、従来の分離大豆蛋白や濃縮大豆蛋白等の大豆素材では得難かった食感の良さや食用組成物の原料生地への分散性、保形性の良さ等の様々な良好な物性を有する粉末状大豆素材を提供できる。これによって食用組成物の物性に関する従来の欠点を改良したり、食用組成物本来の物性を損ねることなく食用組成物中に蛋白質や食物繊維を栄養的に強化することが可能となる。さらには、コレステロール低減効果や腎症予防効果、腸内環境改善効果など、生理機能の発現を目的とする食用組成物に用いる蛋白質又は食物繊維の強化用原料として提供することができ、かかる食用組成物にあってはその物性を損ねることなく蛋白質あるいは食物繊維を強化することが可能となる。 According to the present invention, various good physical properties such as good texture, dispersibility of edible compositions in raw material dough, good shape retention, etc., which were difficult to obtain with conventional soybean materials such as isolated soybean protein and concentrated soybean protein, etc. The powdery soybean material which has can be provided. As a result, it is possible to improve the conventional drawbacks related to the physical properties of the edible composition, and to nutritionally strengthen proteins and dietary fibers in the edible composition without deteriorating the original physical properties of the edible composition. Furthermore, it can be provided as a raw material for reinforcing protein or dietary fiber used in edible compositions for the purpose of manifesting physiological functions, such as cholesterol-reducing effects, nephropathy-preventing effects, intestinal environment-improving effects, and the like. In the case of things, it becomes possible to reinforce protein or dietary fiber without damaging the physical properties.
ポリアクリルアミドゲル電気泳動に供した試料の各蛋白質成分のバンドを染色した図である。分子量マーカーのバンドの位置との関係から特定された各蛋白質成分名を示している。左から1番目のウェルが分子量マーカー、左から2~5番目のウェルがMSPの濃度計算のための検量線作成用標準試料、右から1,2番目のウェルが測定試料である。It is the figure which dye | stained the band of each protein component of the sample used for polyacrylamide gel electrophoresis. Each protein component name specified from the relationship with the band position of the molecular weight marker is shown. The first well from the left is a molecular weight marker, the second to fifth wells from the left are standard samples for preparing a calibration curve for calculating the concentration of MSP, and the first and second wells from the right are measurement samples.
〔用語説明〕
 本発明について説明するに際し、必要な用語についてまず説明する。
〔Glossary〕
In describing the present invention, the necessary terms will be described first.
(7S蛋白質/11S蛋白質/酸沈殿性蛋白質)
 大豆に含まれる蛋白質のうち、7S蛋白質と11S蛋白質は、いずれも大豆のプロテインボディーに貯蔵される主要な貯蔵蛋白質である。
 「7S蛋白質」はβ-コングリシニンとも呼ばれ、一般には3種のサブユニット(α’、α、β)から構成される糖蛋白質であるが、何れかのサブユニットが欠損していても良い。これらのサブユニットはランダムに組み合わされ、3量体を形成している。等電点はpH4.8付近で分子量は17万程度である。
 一方、「11S蛋白質」はグリシニンとも呼ばれ、酸性サブユニット(AS)と塩基性サブユニット(BS)がジスルフィド結合によって結合し、それらが6分子集まった12量体を形成しており、分子量は36万程度である。以下、それぞれ「7S」、「11S」と略記することがある。
(7S protein / 11S protein / acid-precipitating protein)
Among the proteins contained in soybean, 7S protein and 11S protein are both main storage proteins stored in the protein body of soybean.
“7S protein” is also called β-conglycinin and is generally a glycoprotein composed of three types of subunits (α ′, α, β), but any subunit may be missing. These subunits are randomly combined to form a trimer. The isoelectric point is around pH 4.8 and the molecular weight is about 170,000.
On the other hand, “11S protein” is also called glycinin, and acidic subunit (AS) and basic subunit (BS) are linked by disulfide bond to form a 12-mer consisting of 6 molecules. About 360,000. Hereinafter, they may be abbreviated as “7S” and “11S”, respectively.
 7Sと11Sはいずれも酸沈殿性大豆蛋白質である。ここでいう「酸沈殿性蛋白質」は、大豆に含まれる蛋白質の内、脱脂豆乳などの溶液のpHを等電点付近(pH4~6)に調整することにより不溶化し、酸沈澱する性質を有する蛋白質をいう。したがって、例えば分離大豆蛋白に含まれる蛋白質がこれに相当し、分離大豆蛋白製造時に酸沈殿しないホエー中の蛋白質はこれに含まれない。
 7Sと11Sは、品種によっても異なると考えられるが、SDSポリアクリルアミドゲル電気泳動(以下、「SDS-PAGE」と称する。)(図1参照)において泳動後のゲルを染色剤であるクマシーブリリアントブルー(CBB)にて染色した後、デンシトメトリーによってピーク面積を測定した場合、従来の分離大豆蛋白(SPI)などでは大豆蛋白質全体の約70%を占める主要な蛋白質である。そこで以下、7Sと11Sを総称して「MSP」(Major Soy Protein)と称する。
Both 7S and 11S are acid-precipitated soy proteins. The “acid-precipitating protein” as used herein has a property of insolubilizing and precipitating acid by adjusting the pH of a solution such as defatted soymilk in the vicinity of the isoelectric point (pH 4 to 6) among proteins contained in soybean. It refers to protein. Therefore, for example, the protein contained in the separated soy protein corresponds to this, and the protein in whey that does not undergo acid precipitation during the production of the separated soy protein is not included.
7S and 11S are considered to be different depending on the cultivar, but the gel after electrophoresis in SDS polyacrylamide gel electrophoresis (hereinafter referred to as “SDS-PAGE”) (see FIG. 1) is Coomassie Brilliant Blue. When the peak area is measured by densitometry after staining with (CBB), the conventional separated soybean protein (SPI) is a major protein that accounts for about 70% of the whole soybean protein. Therefore, hereinafter, 7S and 11S are collectively referred to as “MSP” (Major Soy Protein).
(脂質親和性蛋白質)
 脂質親和性蛋白質(Lipophilic Proteins)は、大豆の酸沈殿性蛋白質の内、7Sと11S以外のマイナーな酸沈殿性蛋白質群をいい、レシチンや糖脂質などの極性脂質を多く随伴するものである。主に膜成分に含まれる大豆膜蛋白質および膜成分に親和性の高い蛋白質がコロイド状に分散しており、酸性で不溶化する。以下、脂質親和性蛋白質を単に「LP」と略記することがある。
 このLP中にはSDS-PAGEによる推定分子量において主に34kDa、24kDa、18kDa、17kDaを示す蛋白質、リポキシゲナーゼ、γ-コングリシニンや、その他多くの雑多な蛋白質が含まれる。このLPはSDS-PAGEでは7Sや11Sに比べて染色されにくい性質を有している。
(Lipophilic protein)
Lipophilic proteins are a group of minor acid-precipitating proteins other than 7S and 11S among soybean acid-precipitating proteins, and are accompanied by a large amount of polar lipids such as lecithin and glycolipid. Mainly the soybean membrane protein contained in the membrane component and the protein with high affinity to the membrane component are dispersed in a colloidal form and become acidic and insoluble. Hereinafter, the lipophilic protein may be simply abbreviated as “LP”.
This LP mainly includes proteins showing 34 kDa, 24 kDa, 18 kDa and 17 kDa in estimated molecular weights by SDS-PAGE, lipoxygenase, γ-conglycinin and many other miscellaneous proteins. This LP has the property that SDS-PAGE is harder to stain than 7S and 11S.
〔実施形態〕
(粉末状大豆素材)
 本発明の粉末状大豆素材は、大豆蛋白質及び大豆食物繊維を含有し、蛋白質含量が無脂固形分重量あたり35~85重量%であって、蛋白質のLCI値が60以上であることを特徴とするものである。
 換言すれば、該大豆素材は酸沈殿性蛋白質の内、7Sや11Sとも異なるLPが特に濃縮された大豆蛋白質と大豆食物繊維が主体となっているものである。これについてより詳細に説明する。
Embodiment
(Powdered soybean material)
The powdery soy material of the present invention comprises soy protein and soy dietary fiber, the protein content is 35 to 85% by weight per non-fat solid content, and the LCI value of the protein is 60 or more. To do.
In other words, the soy material is mainly composed of soy protein and soy dietary fiber in which LP different from 7S and 11S is particularly concentrated among acid-precipitating proteins. This will be described in more detail.
<大豆蛋白質>
・蛋白質含量
 本発明の粉末状大豆素材中の蛋白質含量は、無脂固形分あたり35~85重量%であることが重要であり、42~68重量%であるのがより好ましく、45~65重量%がさらに好ましい。ここで「無脂固形分」とは、全固形分からエーテルで抽出される中性脂質を除いた固形分をいう。該大豆素材中の蛋白質含量が少なすぎると相対的に食物繊維含量が多くなり、食感がざらつきやすやぱさつきが多くなり、乳化安定性も低下する傾向となり、逆に多すぎるとLPに随伴する脂質の量が多くなり、風味が劣化しやすくなり好ましくない。なお、蛋白質含量はケルダール法によって定量した窒素量に、「窒素-蛋白質換算係数」(6.25)を乗じて算出される。
<Soy protein>
Protein content It is important that the protein content in the powdered soybean material of the present invention is 35 to 85% by weight per non-fat solid content, more preferably 42 to 68% by weight, and 45 to 65% by weight. % Is more preferable. Here, the “non-fat solid content” refers to a solid content obtained by removing neutral lipids extracted with ether from the total solid content. If the protein content in the soybean material is too small, the dietary fiber content will be relatively increased, the texture will be rough and the texture will increase, and the emulsification stability will tend to decrease. This is not preferable because the amount of lipid increases and the flavor tends to deteriorate. The protein content is calculated by multiplying the nitrogen amount determined by the Kjeldahl method by the “nitrogen-protein conversion factor” (6.25).
・LP含量
 本発明の粉末状大豆素材に含まれる大豆蛋白質の組成は、少なくともLPが通常の分離大豆蛋白、濃縮大豆蛋白、脱脂大豆、オカラ等の大豆素材に含まれる大豆蛋白質の組成よりも濃縮されていることが重要である。
 LPは酸沈殿性蛋白質の内、7S及び11S以外の雑多な蛋白質が混在したものであるが故に、各々の蛋白質を全て特定することは困難である。また分離大豆蛋白等の大豆素材は最終の製品化工程において一般的には加熱殺菌されるため、7S,11SはLPと共に加熱変性が起こっている。そのため、製品化された分離大豆蛋白等からLPを7Sや11Sから分離し、LP含量を測定することが困難である。また、一般的な蛋白質組成の測定方法であるSDS-PAGEではLPが7Sや11Sに比べてCBB染色がされにくいという性質を有し、染色度によって正確に測定することも困難である。
LP content The composition of the soy protein contained in the powdered soy material of the present invention is at least LP more concentrated than the composition of the soy protein contained in the soy material such as ordinary separated soy protein, concentrated soy protein, defatted soy, and okara. It is important that
Since LP is a mixture of various proteins other than 7S and 11S among acid-precipitating proteins, it is difficult to specify all the proteins. In addition, since soybean materials such as separated soybean protein are generally heat-sterilized in the final productization process, 7S and 11S are heat-denatured together with LP. For this reason, it is difficult to measure LP content by separating LP from 7S and 11S from commercialized separated soybean protein and the like. In addition, SDS-PAGE, which is a general method for measuring protein composition, has the property that LP is less susceptible to CBB staining than 7S and 11S, and it is difficult to accurately measure the degree of staining.
 そこで、このLPの濃縮度合いは、LP含量の推定値として「LCI値」という指標を用いることとする。LCI値は、簡易的に7S,11S,LPの各蛋白質中の主要な蛋白質を選択し、それらの染色比率を求め、これらの比率からLP含量を推定する以下のLCI値の算出方法により算出されるものである。なお、本方法は一般的な分離大豆蛋白、濃縮大豆蛋白などの大豆素材のみならず、大豆7S蛋白質、大豆11S蛋白質、LPの多いSPIなどの各種分画物にも広く適用が可能である。 Therefore, for the degree of concentration of LP, an index called “LCI value” is used as an estimated value of LP content. The LCI value is calculated by the following LCI value calculation method in which the main protein in each of the 7S, 11S, and LP proteins is simply selected, their staining ratio is obtained, and the LP content is estimated from these ratios. Is. Note that this method can be widely applied not only to soy materials such as general separated soy protein and concentrated soy protein, but also to various fractions such as soy 7S protein, soy 11S protein, and SPI with high LP.
-LCI値の算出方法-
(a)各蛋白質中の主要な蛋白質として、7Sはαサブユニット及びα'サブユニット(α+α')、11Sは酸性サブユニット(AS)、LPは34kDa蛋白質及びリポキシゲナーゼ(P34+Lx)を選択し、SDS-PAGEにより選択された各蛋白質の染色比率を求める(図1参照)。SDS-PAGEは表1の条件で行うものとする。
(b)数1の式によりLP推定含量(Lipophilic Proteins Content Index、以下「LCI」と略する。)を算出する。
-Calculation method of LCI value-
(A) As main proteins in each protein, 7S selects α subunit and α ′ subunit (α + α ′), 11S selects acidic subunit (AS), LP selects 34 kDa protein and lipoxygenase (P34 + Lx), and SDS Determine the staining ratio of each protein selected by PAGE (see FIG. 1). SDS-PAGE shall be performed under the conditions shown in Table 1.
(B) The estimated LP content (Lipophilic Proteins Content Index, hereinafter abbreviated as “LCI”) is calculated according to the equation (1).
(表1)
Figure JPOXMLDOC01-appb-I000001
(Table 1)
Figure JPOXMLDOC01-appb-I000001
(数1)
Figure JPOXMLDOC01-appb-I000002
(Equation 1)
Figure JPOXMLDOC01-appb-I000002
 上記の方法により算出される、本発明の粉末状大豆素材に含まれる蛋白質のLCI値は、60以上であることが重要である。このLCI値はさらに62以上が好ましく、65以上がより好ましく、70以上がさらに好ましい。なお通常のLPが濃縮されていない大豆素材の場合、LCI値は60未満であるので、該大豆素材のLCI値が60未満となると、通常の大豆素材と品質的に変わらなくなる。ちなみに本発明者らの分析によれば、従来製法による分離大豆蛋白のLCI値は35~50程度に過ぎない。また、通常の脱脂大豆から蛋白質を水抽出した不溶性残渣であるオカラの場合、LP含量は乾燥固形分中20~30重量%程度であり、そのLCI値は50程度に過ぎない。このようにLCI値が60以上である該大豆素材は、LPが濃縮されているものであるため、他の酸沈殿性大豆蛋白質である7S蛋白質と11S蛋白質の組成は通常の大豆素材と比べて相対的に低いものである。そのため、7S蛋白質と11S蛋白質の組成を限定することは特に不要であるが、これらの組成についてはより具体的には以下の通りである。 It is important that the LCI value of the protein contained in the powdered soybean material of the present invention calculated by the above method is 60 or more. The LCI value is preferably 62 or more, more preferably 65 or more, and further preferably 70 or more. In the case of a soybean material in which normal LP is not concentrated, the LCI value is less than 60. Therefore, when the LCI value of the soybean material is less than 60, the quality does not change from that of a normal soybean material. By the way, according to the analysis by the present inventors, the LCI value of the isolated soybean protein obtained by the conventional production method is only about 35-50. Further, in the case of okara, which is an insoluble residue obtained by extracting protein from normal defatted soybean with water, the LP content is about 20 to 30% by weight in the dry solid content, and the LCI value is only about 50. Since the soybean material having an LCI value of 60 or more is concentrated in LP, the composition of 7S protein and 11S protein, which are other acid-precipitated soybean proteins, is compared with that of ordinary soybean materials. It is relatively low. Therefore, it is not particularly necessary to limit the composition of the 7S protein and the 11S protein, but more specifically, these compositions are as follows.
・7S蛋白質及び11S蛋白質(MSP)含量
 本発明の粉末状大豆素材における蛋白質あたりのMSP含量は、LPの割合が減るほど、すなわちLCI値が低くなるほど多くなる。該大豆素材中にMSPが多く含まれていると、通常の濃縮大豆蛋白の組成に近づき、ネチャつきや硬い食感などの特徴が表れ、好ましくない。そのため、できるだけ含まれていないことが好ましい。MSP含量はLCI値と逆相関の関係にあるため特に限定は不要であるが、より詳細に示すならば、大豆蛋白質あたり40重量%以下が好ましく、30重量%以下がより好ましく、20重量%以下がさらに好ましい。
 なお、MSP含量について測定する場合は、以下の方法によってSDS-PAGEを用いて測定することとする。
-7S protein and 11S protein (MSP) content The MSP content per protein in the powdered soybean material of the present invention increases as the proportion of LP decreases, that is, the LCI value decreases. If the soybean material contains a lot of MSP, it is not preferable because it approximates the composition of a normal concentrated soy protein and exhibits features such as stickiness and hard texture. Therefore, it is preferable that it is not contained as much as possible. The MSP content is inversely correlated with the LCI value and is not particularly limited. However, in more detail, it is preferably 40% by weight or less per soy protein, more preferably 30% by weight or less, and 20% by weight or less. Is more preferable.
In addition, when measuring about MSP content, it shall measure using SDS-PAGE by the following methods.
-MSP含量算出方法-
 脱脂大豆から水抽出した抽出液に硫酸ナトリウムを1Mの濃度になるように溶解し、さらに10mMになるように還元剤である亜硫酸ナトリウムを加え、pHを塩酸か硫酸で4.5に調整し、不溶物を除いた後、透析してイオン強度を0.03以下にすることによって生じる沈殿物を集めることによって得られた画分を「標準精製MSP」とする。
 この標準精製MSPの蛋白質含量をケルダール法によって求め、蛋白質量として5~15μgをSDS-PAGE用ゲルの各ウェルにアプライし、電気泳動後、CBB染色し(図1参照)、デンシトメーターによって、その染色度をカウントする。そして蛋白質アプライ量と染色度の関係を基に検量線を作成する。同一のスラブゲル上に測定試料をアプライし展開する。測定試料には、MSP以外にLPなどが存在するため、デンシトメトリーにてMSPのバンド(αサブユニット、α’サブユニット、βサブユニット、酸性サブユニット、塩基性サブユニット)の染色度だけを合算し、その染色程度から測定試料に含まれるMSPの含量をMSP検量線から測定する。
-MSP content calculation method-
Sodium sulfate is dissolved in the extract extracted from defatted soybean with water to a concentration of 1M, sodium sulfite as a reducing agent is added to a concentration of 10 mM, pH is adjusted to 4.5 with hydrochloric acid or sulfuric acid, and insoluble matter is added. And the fraction obtained by collecting the precipitate produced by dialysis to bring the ionic strength to 0.03 or less is referred to as “standard purified MSP”.
The protein content of this standard purified MSP was determined by the Kjeldahl method, 5-15 μg of protein mass was applied to each well of the SDS-PAGE gel, electrophoresed, and then CBB stained (see FIG. 1). The degree of staining is counted. A calibration curve is created based on the relationship between the amount of protein applied and the degree of staining. A measurement sample is applied and spread on the same slab gel. Since LP and other substances are present in addition to MSP in the measurement sample, only the degree of staining of the MSP band (α subunit, α ′ subunit, β subunit, acidic subunit, basic subunit) is determined by densitometry. From the degree of staining, the content of MSP contained in the measurement sample is measured from the MSP calibration curve.
<大豆食物繊維>
 本発明の粉末状大豆素材は大豆食物繊維を含むことが必須である。すなわち、LPと大豆食物繊維が共存していることが特徴であり、これらの組合せが大豆素材としての特有の効果を発揮させる。該大豆素材中の大豆食物繊維の含量は、蛋白質に対して下限は通常15重量%以上であり、20重量%以上が好ましく、25重量%以上がより好ましく、30重量%以上がさらに好ましい。また該大豆素材中の大豆食物繊維の含量の上限は蛋白質に対して通常130重量%以下であり、100重量%以下が好ましく、95重量%以下がより好ましく、90重量%以下がさらに好ましい。
 なお、食物繊維の含量は、「五訂増補日本食品標準成分表」(文部科学省、2005)に準ずるものとし、食物繊維含量は酵素-重量法(プロスキー変法)により測定する。
 大豆食物繊維が含まれないか少なすぎ、該大豆素材中の蛋白質含量が85重量%を超えてしまうと、LPが大部分を占める構成となり、LP特有の劣化臭のような風味が顕著となり、分散性や保形性等の物性も低下する傾向となる。逆に大豆食物繊維が多すぎ、蛋白質含量が35重量%未満となると、食感にざらつきやぱさつきが生じやすくなり、乳化安定性等の物性も低下する傾向となる。
<Soybean dietary fiber>
The powdery soybean material of the present invention must contain soybean dietary fiber. That is, LP and soybean dietary fiber coexist, and the combination of these exhibits a unique effect as a soybean material. The lower limit of the content of soybean dietary fiber in the soybean material is usually 15% by weight or more, preferably 20% by weight or more, more preferably 25% by weight or more, and further preferably 30% by weight or more with respect to protein. Moreover, the upper limit of the content of soybean dietary fiber in the soybean material is usually 130% by weight or less, preferably 100% by weight or less, more preferably 95% by weight or less, and still more preferably 90% by weight or less based on protein.
The dietary fiber content shall be in accordance with the “Fiveth Amendment Japanese Food Standard Ingredients Table” (Ministry of Education, Culture, Sports, Science and Technology, 2005), and the dietary fiber content is measured by the enzyme-weight method (Prosky modified method).
If soy dietary fiber is not contained or is too little, and the protein content in the soy material exceeds 85% by weight, the LP occupies most of the composition, and the flavor such as LP-specific deteriorated odor becomes remarkable. Physical properties such as dispersibility and shape retention tend to decrease. On the other hand, if the soybean fiber is too much and the protein content is less than 35% by weight, the texture is likely to be rough and crunchy, and the physical properties such as emulsion stability tend to decrease.
<脂質>
 食用組成物中の脂質は、エーテル抽出法で抽出される中性脂質と、エーテル抽出法では抽出されず、クロロホルム:メタノール=2:1の混合溶媒で抽出される極性脂質とに分類される。
 粉末状大豆素材は中性脂質をできるだけ含まないことが好ましく、中性脂質は通常3重量%以下であるのが好ましい。
 一方、極性脂質はLPと随伴する性質を有するため、該大豆素材中に通常5~15重量%含まれる場合が多い。なお、極性脂質含量はクロロホルム-メタノール抽出法で測定される脂質(中性脂質及び極性脂質)の含量からエーテル抽出法で抽出される脂質(中性脂質)の含量を差し引いた値として算出される。
<Lipid>
Lipids in the edible composition are classified into neutral lipids extracted by the ether extraction method and polar lipids extracted by a mixed solvent of chloroform: methanol = 2: 1 without being extracted by the ether extraction method.
It is preferable that the powdery soybean material contains as little neutral lipid as possible, and the neutral lipid is usually preferably 3% by weight or less.
On the other hand, since polar lipids have the properties associated with LP, they are usually contained in the soybean material in an amount of usually 5 to 15% by weight. The polar lipid content is calculated by subtracting the lipid (neutral lipid) content extracted by the ether extraction method from the lipid (neutral lipid and polar lipid) content measured by the chloroform-methanol extraction method. .
<ホエー成分>
 粉末状大豆素材にはホエー成分である糖質や酸可溶性大豆蛋白質(pH4~6で酸沈殿しない蛋白質)は実質的に含まないのが好ましい。すなわち、該大豆素材の製造時において、原料からホエー成分が抽出除去されていることが好ましい。該大豆素材にホエー成分が多く含まれると食物繊維の含量が相対的に低下して、乳化性や保水性が悪くなる。
<Whey ingredients>
It is preferable that the powdery soy material does not substantially contain whey component sugar or acid-soluble soy protein (protein that does not undergo acid precipitation at pH 4 to 6). That is, it is preferable that the whey component is extracted and removed from the raw material during the production of the soybean material. If the soy material contains a large amount of whey component, the content of dietary fiber is relatively lowered, and the emulsifiability and water retention are deteriorated.
(粉末状大豆素材の調製)
 本発明の粉末状大豆素材の調製例を示す。なお、以下の調製例はあくまで例示に過ぎず、本発明の粉末状大豆素材が特定するように、大豆蛋白質と大豆食物繊維を共に含有し、特定の蛋白質含量と特定のLCI値を満たすものであれば特に限定されるものではない。
(Preparation of powdered soybean material)
The preparation example of the powdery soybean raw material of this invention is shown. It should be noted that the following preparation examples are merely illustrative, and contain both soy protein and soy dietary fiber and satisfy a specific protein content and a specific LCI value as specified by the powdered soybean material of the present invention. There is no particular limitation as long as it is present.
 まず本大豆素材の原料は、全脂大豆又は脱脂大豆のいずれを用いることもでき、本大豆素材中の蛋白質含量を高めたい場合には脱脂大豆を用いる方が好ましい。
 また大豆としては通常の大豆の他、育種あるいは遺伝子操作によって7S又は/及び11Sの一部もしくは全部を欠損させた大豆を原料に用いることできる。
First, as the raw material of the soybean material, either full-fat soybean or defatted soybean can be used. When it is desired to increase the protein content in the soybean material, it is preferable to use the defatted soybean.
In addition to normal soybeans, soybeans that are partially or entirely deficient in 7S and / or 11S by breeding or genetic manipulation can be used as raw materials.
 これらの原料を用いて本発明の大豆素材を調製する一つの方法としては、LPを選択的に水不溶化させて、7S及び11Sと分画してLPを濃縮する方法を用いることができる。例えば脱脂大豆又は全脂大豆を原料として特許文献1(WO2006/129647号公報)に記載される方法を利用できる。すなわち、大豆を加熱変性処理あるいはアルコール変性処理し、PDIが40以上80未満の加工大豆を得、これを必要により粉砕し、水で抽出し、その不溶性画分を回収し、これを乾燥粉末化したものを用いることができる。 As one method for preparing the soybean material of the present invention using these raw materials, a method in which LP is selectively insolubilized and fractionated with 7S and 11S to concentrate LP can be used. For example, a method described in Patent Document 1 (WO 2006/129647) using defatted soybeans or whole fat soybeans as a raw material can be used. That is, soybean is heat-denatured or alcohol-denatured to obtain a processed soybean having a PDI of 40 or more and less than 80, which is pulverized if necessary, extracted with water, and its insoluble fraction is recovered and dried into powder. Can be used.
 特許文献1の方法をより具体的に説明すると、大豆のPDIを40以上80未満という特定の範囲に調整することにより、7S及び11Sをできるだけ不溶化することなく、LPを選択的に水不溶化することができる。ここで「PDI」とは蛋白質分散性指数(Protein Dispersibility Index)の略称であり、AOCS公式法(Ba10-65)として記載されている大豆製品中の溶解分散する(Dispersible)蛋白質を一定条件下で測定することにより得られる指数である。 The method of Patent Document 1 will be described more specifically. By adjusting the PDI of soybean to a specific range of 40 or more and less than 80, LP is selectively water-insolubilized without making 7S and 11S as insoluble as possible. Can do. Here, “PDI” is an abbreviation for Protein Dispersibility Index, and the Dispersible protein in soybean products described as the AOCS official method (Ba10-65) under certain conditions. It is an index obtained by measuring.
 PDIを調整するための加熱変性処理としては、焙煎装置、熱風加熱装置、マイクロ波加熱装置等を使用する乾式加熱方式や、加湿加熱装置、蒸煮装置、蒸気加熱装置等を使用する湿式加熱方式を特に限定されることなく採用することができる。ただし水が大豆に浸るような状態で加熱すると蛋白質が抽出されてしまうため、避けた方が良い。一例として、大豆を密閉タンクに封入し、相対湿度90%以上の雰囲気下で品温が70~95℃程度になるように密閉タンクの外側を覆うジャケット内を加熱する方法などが採用できる。この場合の加熱の温度や時間の条件はLPの不溶化が選択的なものとなる限り特に限定されないが、通常は品温で60~95℃となるよう温度設定し、時間は1分~10時間の間で行うことが適当である。他の加熱方法として、過熱水蒸気による加熱装置、エクストルーダー、ジェットクッカー等の加熱装置を使用することもできる。 As a heat denaturation treatment for adjusting PDI, a dry heating method using a roasting device, a hot air heating device, a microwave heating device, etc., or a wet heating method using a humidification heating device, a steaming device, a steam heating device, etc. Can be employed without any particular limitation. However, it is better to avoid heating because the protein is extracted when the water is soaked in soybeans. As an example, a method in which soybean is enclosed in a sealed tank and the inside of the jacket covering the outside of the sealed tank is heated so that the product temperature is about 70 to 95 ° C. in an atmosphere with a relative humidity of 90% or more can be employed. The heating temperature and time conditions in this case are not particularly limited as long as LP insolubilization is selective, but the temperature is usually set to 60 to 95 ° C., and the time is 1 minute to 10 hours. It is appropriate to perform between. As another heating method, a heating device such as a heating device using superheated steam, an extruder, or a jet cooker may be used.
 PDIを調整するための変性方法において、加熱以外の態様として、アルコール変性処理を行う場合、蛋白質及びオカラ成分を含む原料大豆に対し、等重量以下、好ましくは2~100重量部、より好ましくは8~20重量部、さらに好ましくは10~15重量部の極性アルコール溶液を添加し、含浸させる方法が好ましい。なお、この方法は従来のアルコール洗浄による濃縮大豆蛋白などの製法のように、大豆を何倍量ものアルコールを浸漬し、懸濁状態にして大豆の糖質などの非蛋白質成分を洗浄する方法とは全く考え方が異なり、大豆に対して等重量以下の極性溶媒溶液を添加し、含浸させるものである。この場合、混合された大豆の状態は典型的には湿潤した粉末状態となる。ただし、極性溶媒の添加量が大豆に対して2重量%よりも低くなると、LPの選択的な水不溶化が不十分となるので、水抽出時のLPの抽出抑制効果が不充分となる傾向となる。逆に等重量よりも多くなると、LPと共に7Sと11Sも水不溶化する非選択的な水不溶化が起こりやすくなり、7Sと11Sの抽出までが不十分となる。LPの選択的水不溶化を促進するために適する極性溶媒としては、極性アルコール溶液(メタノール、エタノール、プロパノール、イソプロパノール等)を用いることができる。特に、食品工業上多用されているエタノール水溶液を使用することが好ましい。水は純水のままでも良いし、酸水溶液(塩酸水溶液、炭酸水溶液、クエン酸水溶液等)、アルカリ水溶液(水酸化ナトリウム溶液、重炭酸ナトリウム等)等を使用することもできる。極性溶媒溶液の濃度は5~100%が好ましく、50~80%がより好ましい。極性溶媒溶液の濃度が低すぎても高すぎても、LPの変性による水不溶化が不十分となる。極性溶媒溶液の添加方法は、例えば噴霧により粉に吹付ける方法や、滴下する方法などで実施できるが、特に限定されない。極性溶媒溶液添加後の混合方法は、例えばニーダーのような攪拌機や、高速攪拌機などを用いることができる。さらに、上記のアルコール含浸処理に加え、加温処理を併用することがより好ましい。加温温度は、大豆の品温で30~95℃が好ましく、40~90℃がより好ましい。また加温時間は5~100分が好ましく、10分~60分がより好ましい。極性溶媒の含浸処理と加温処理を併用する場合、これらの工程の順序には特に限定されないが、極性溶媒を添加し、混合した後に加温処理を施すか、極性溶媒を添加・混合しながら加熱処理を行うのが好ましい。この極性溶媒の含浸処理と加温処理を併用することにより、比較的低い温度処理でも効率よく大豆中のLPのみを選択的に水不溶化することが可能となる。また加熱による色や臭いの発生も抑え、加工大豆やそれを原料に調製される調製品の風味を向上することが可能となる。さらに、極性溶媒の添加量を少なくすることができるので、処理後の極性溶媒の除去工程が従来のアルコール洗浄法などと比較すると極めて容易となり、効率的な製造プロセスを確立する上で有利である。加工大豆に残存する極性溶媒はほとんど加温処理によって揮発させることができ、これを直接抽出工程に供することができるが、所望によりさらに残存量を低下させたい場合には、さらに品温40~60℃、減圧(-10mmHg程度)下で10分~60分の処理を行えば完全に揮発させることが出来、ほぼ添加前の大豆の重量に戻すことができる。なお、揮発させた極性溶媒は蒸留により回収すれば、再利用が可能であるので、製造プロセス上有利である。 In the modification method for adjusting PDI, as an aspect other than heating, when alcohol modification treatment is performed, the equivalent weight or less, preferably 2 to 100 parts by weight, more preferably 8 parts by weight with respect to raw soybean containing protein and okara components. A method of adding and impregnating ˜20 parts by weight, more preferably 10 to 15 parts by weight of a polar alcohol solution is preferred. This method is a method for washing non-protein components such as saccharides of soybeans by immersing soybeans in many times the amount of alcohol and suspending them, as in the conventional method of producing concentrated soy protein by alcohol washing. The idea is completely different, and an equal weight or less polar solvent solution is added to and impregnated with soybean. In this case, the state of the mixed soybean is typically a wet powder state. However, when the addition amount of the polar solvent is lower than 2% by weight with respect to soybean, the selective water insolubilization of LP becomes insufficient, so that the effect of suppressing the extraction of LP during water extraction tends to be insufficient. Become. On the other hand, when the weight is greater than the equal weight, non-selective water insolubilization in which 7S and 11S are insolubilized with LP tends to occur, and the extraction of 7S and 11S becomes insufficient. As a polar solvent suitable for promoting selective water insolubilization of LP, a polar alcohol solution (methanol, ethanol, propanol, isopropanol, etc.) can be used. In particular, it is preferable to use an aqueous ethanol solution frequently used in the food industry. The water may be pure water, or an acid aqueous solution (hydrochloric acid aqueous solution, carbonic acid aqueous solution, citric acid aqueous solution, etc.), alkaline aqueous solution (sodium hydroxide solution, sodium bicarbonate, etc.), etc. may be used. The concentration of the polar solvent solution is preferably 5 to 100%, more preferably 50 to 80%. If the concentration of the polar solvent solution is too low or too high, water insolubilization due to the denaturation of LP becomes insufficient. The method for adding the polar solvent solution can be carried out by, for example, a method of spraying the powder by spraying or a method of dropping, but is not particularly limited. As a mixing method after adding the polar solvent solution, for example, a stirrer such as a kneader or a high-speed stirrer can be used. Further, in addition to the above-described alcohol impregnation treatment, it is more preferable to use a heating treatment in combination. The heating temperature is preferably 30 to 95 ° C, more preferably 40 to 90 ° C, as the product temperature of soybean. The heating time is preferably 5 to 100 minutes, more preferably 10 to 60 minutes. When using both the impregnation treatment with a polar solvent and the heating treatment, the order of these steps is not particularly limited. However, the polar solvent is added and mixed and then the heating treatment is performed, or the polar solvent is added and mixed. Heat treatment is preferably performed. By using this polar solvent impregnation treatment and heating treatment together, it becomes possible to selectively insolubilize only LP in soybean efficiently and efficiently even at a relatively low temperature treatment. In addition, generation of color and odor due to heating can be suppressed, and the flavor of processed soybeans and preparations prepared from such soybeans can be improved. Furthermore, since the amount of the polar solvent added can be reduced, the process for removing the polar solvent after the treatment becomes extremely easy as compared with the conventional alcohol washing method, etc., which is advantageous in establishing an efficient manufacturing process. . Most of the polar solvent remaining in the processed soybean can be volatilized by heating treatment, and this can be directly applied to the extraction process. However, if it is desired to further reduce the residual amount, if desired, the product temperature is 40-60. If treated for 10 to 60 minutes at ℃ and reduced pressure (about -10mmHg), it can be completely volatilized and returned to the weight of soybean before addition. The volatilized polar solvent is advantageous in terms of the manufacturing process because it can be reused if it is recovered by distillation.
 以上のようにして得られた加工大豆は必要により粉砕し、2~10倍量の水を加え、撹拌して懸濁させることにより水抽出すると、7S及び11Sが積極的に水溶性画分側に抽出される。この水溶性画分を遠心分離やろ過等の固液分離により除去し、残りの不溶性画分を回収する。この不溶性画分は不溶化したLPと大豆食物繊維が主体となっており、相対的にLPが濃縮されており、LCI値が60以上となる。なお、水抽出の際のpHは通常中性~アルカリ性(pH7~12)で行うが、粉末状大豆素材の蛋白質含量をできるだけ上げたい場合には、水抽出の際に懸濁液のpHを酸性側(pH7未満)に調整することにより、水溶性画分側に溶解していた蛋白質を不溶性画分側へ移行させることができる。ただし、大豆蛋白質の等電点にpHを近づけるほど7S及び11Sが不溶性画分側へ移行し、LCI値が低下するため、LCI値が60未満とならないよう留意が必要であり、条件にもよるがpHを酸性側へ調整する場合でも通常はpHを5以上、好ましくは5.5以上にすることが適当である。 The processed soybeans obtained as described above are pulverized if necessary, added with 2 to 10 times the amount of water, stirred and suspended to extract water, and 7S and 11S are positively added to the water-soluble fraction side. Extracted into This water-soluble fraction is removed by solid-liquid separation such as centrifugation or filtration, and the remaining insoluble fraction is recovered. This insoluble fraction is mainly composed of insolubilized LP and soybean dietary fiber, and the LP is relatively concentrated, and the LCI value is 60 or more. The pH at the time of water extraction is usually neutral to alkaline (pH 7 to 12), but if you want to increase the protein content of the powdered soybean material as much as possible, the pH of the suspension should be acidic at the time of water extraction. By adjusting to the side (less than pH 7), the protein dissolved in the water-soluble fraction side can be transferred to the insoluble fraction side. However, 7S and 11S move to the insoluble fraction side as the pH approaches the isoelectric point of soy protein, and the LCI value decreases. Therefore, care must be taken so that the LCI value does not become less than 60, depending on the conditions. Even when the pH is adjusted to the acidic side, it is usually appropriate to adjust the pH to 5 or more, preferably 5.5 or more.
 以上で得られた不溶性画分を最終的に乾燥粉末化し、本発明の粉末状大豆素材が得られる。なお、乾燥粉末化はスプレードライヤーやフリーズドライ等の公知の乾燥方法を用いることができる。また乾燥粉末化の前に、要すればホモゲナイザー等により微細化処理を行ったり、加圧加熱処理によって食物繊維を水溶化したり、製造工程中において不溶性食物繊維を一部除去したり、加熱殺菌処理を行ったりすることができる。また乾燥粉末化の後にさらに微粉砕処理を行ったり、造粒処理を行ったりすることもできる。このように、食用組成物への添加適性を考慮し、本発明の粉末状大豆素材に対して所望の付随処理を行うことは自由である。 The insoluble fraction obtained as described above is finally dried and powdered to obtain the powdered soybean material of the present invention. In addition, dry powdering can use well-known drying methods, such as a spray dryer and freeze drying. In addition, before dry powdering, if necessary, it can be refined with a homogenizer, etc., dietary fiber can be made water-soluble by pressure heat treatment, some insoluble dietary fiber can be removed during the manufacturing process, or heat sterilization treatment Can be done. Further, after the dry powderization, a fine pulverization process or a granulation process can be performed. Thus, in consideration of suitability for addition to the edible composition, it is free to perform a desired associated treatment on the powdered soybean material of the present invention.
 分画法を用いずに本発明の大豆素材を調製する別の方法としては、育種あるいは遺伝子操作によって7S又は/及び11Sの一部もしくは全部を欠損させた大豆を原料に用いる方法が挙げられる。これらの大豆は相対的にLPの含量が一般の大豆に比べて高くなっているため、品種にもよるが分画法を用いなくとも該大豆を粉砕して大豆粉としたり、酸洗浄やアルコール洗浄によりホエーを除去して濃縮大豆蛋白としたり、あるいは豆乳を除去してオカラを調製するだけで、本発明の特定の要件を満たす粉末状大豆素材に調製しうる。この場合にも必須ではないがLPが不溶化されて食物繊維と共存していることが好ましく、例えば上記の欠損大豆を原料として大豆粉などに加工して本発明の粉末状大豆素材とする場合には、原料に乾熱処理又は湿熱処理を行い、大豆のPDIを好ましくは40以上80未満に低下させておくとよい。 As another method for preparing the soybean material of the present invention without using the fractionation method, there is a method in which soybeans in which a part or all of 7S and / or 11S has been deleted by breeding or genetic manipulation are used as a raw material. Since these soybeans have a relatively high LP content compared to general soybeans, depending on the variety, the soybeans can be crushed into soy flour without using a fractionation method, or acid washing or alcohol By simply removing whey by washing to obtain concentrated soy protein or preparing okara by removing soy milk, it can be prepared into a powdered soy material that meets the specific requirements of the present invention. In this case as well, it is preferable that LP is insolubilized and coexisting with dietary fiber. For example, when the above-mentioned deficient soybean is processed into soybean powder as a raw material to obtain the powdered soybean material of the present invention. The raw material is subjected to dry heat treatment or wet heat treatment, and the PDI of soybean is preferably reduced to 40 or more and less than 80.
(粉末状大豆素材の食用組成物への使用)
 以上のようにして得られる本発明の粉末状大豆素材は、LPと大豆食物繊維が主体となっていることが特徴である。
 該大豆素材は種々の食用組成物に使用することができ、その食用組成物の種々の物性を改良することが可能である。ここで、物性とは食用組成物が有する分散性、保形性、保水性、乳化安定性、酸化安定性、粘性、可塑性、食感などを総称した性質いう。
 具体的には、該大豆素材を食用組成物に用いた場合、従来の分離大豆蛋白や濃縮大豆蛋白等の既存の大豆素材に比べて、食感が滑らか、ネチャツキが少ない、口当たりが軽い、喉通りが良い等の利点を有しており、既存の大豆素材を用いた場合に比べ、食感の改良された食用組成物に仕上げることが可能であり、また原料の分散性、食用組成物の保形性や乳化安定性の改善や、風味のコクを向上させることができるなど、既存の大豆素材と比較して食用組成物への適用範囲が広い点に特徴を有する。またそれと共にLPが蛋白質組成の主体であるので、該蛋白質の持つ生理機能を高めた食用組成物、および大豆食物繊維との組合せにより、その特性をより生かした食用組成物が調製可能となる。
(Use of powdered soybean material in edible compositions)
The powdery soybean material of the present invention obtained as described above is characterized by being mainly composed of LP and soybean dietary fiber.
The soybean material can be used in various edible compositions, and various physical properties of the edible composition can be improved. Here, the physical properties are general properties of dispersibility, shape retention, water retention, emulsification stability, oxidation stability, viscosity, plasticity, texture, etc. of the edible composition.
Specifically, when the soybean material is used in an edible composition, it has a smooth texture, less stickiness, light mouthfeel, and throat compared to existing soybean materials such as conventional separated soybean protein and concentrated soybean protein. Compared to the case of using an existing soybean material, it can be finished into an edible composition with improved texture, and the dispersibility of raw materials and the edible composition Compared with existing soybean materials, it is characterized by its wide range of application to edible compositions, such as improved shape retention and emulsion stability, and improved flavor. In addition, since LP is the main component of the protein composition, it is possible to prepare an edible composition that enhances the physiological function of the protein and an edible composition that makes better use of its properties by combining with soy dietary fiber.
 これに対して7S蛋白質は加工によっては粘性が高く、ネチャツキの原因になる場合があり、このネチャツキが好ましくない食感となる場合がある。また、11S蛋白質は加工によって、特にカルシウムイオンやマグネシウムイオンなどの塩存在下で加熱すると著しく反応して凝集することから、ざらつきの原因になる場合がある。また酸による凝集性も高く、酸性の食用組成物に利用した場合には、ざらつきや渋味などの味に影響する場合がある。また、一般的に7Sや11S主体の大豆蛋白質のゲルは硬くて弾力があり、加工条件によってはいつまでも口内で溶解せず、喉通りの悪いゲル質になってしまう場合がある。このように加工食品には加工上の理由から好ましくない物性に仕上がる場合があり、これは蛋白質の組成が関係すると考えられる。 On the other hand, 7S protein is highly viscous depending on processing and may cause stickiness, which may cause an unpleasant texture. In addition, 11S protein may cause roughness due to processing, particularly when heated in the presence of a salt such as calcium ion or magnesium ion, because it reacts and aggregates significantly. Moreover, the cohesiveness by an acid is also high, and when it uses for an acidic edible composition, it may affect tastes, such as roughness and astringency. In general, soy protein gels mainly composed of 7S and 11S are hard and elastic, and depending on processing conditions, they may not dissolve in the mouth forever, resulting in poor gel quality in the throat. In this way, processed foods may have undesired physical properties due to processing reasons, which may be related to protein composition.
 従って、LP主体の蛋白質を用いると様々な条件においても急激な変化が起こりにくく、比較的温和な変化を示し、しかも適度な溶解性や乳化性を示すので加工しやすい。pHの変化や、カルシウムイオンやマグネシウムイオンの存在下においてもざらつきが生じにくく良好な食感を維持できる。さらに大豆食物繊維が共存することにより、安定した保形性や保水性を付与することができる。大豆食物繊維だけでは、そのものの持つ食感のモサモサ感があり、乳化力も低く好ましくない。LPと共存することによって、大豆食物繊維の食感が緩和されて滑らかな食感が実現され、乳化力も付与される。 Therefore, when an LP-based protein is used, abrupt changes are unlikely to occur under various conditions, a relatively mild change is exhibited, and moderate solubility and emulsification are exhibited, making it easy to process. Roughness hardly occurs even in the presence of changes in pH and calcium ions or magnesium ions, and a good texture can be maintained. Furthermore, the coexistence of soybean dietary fiber can impart stable shape retention and water retention. Soybean dietary fiber alone is not preferable because it has the texture of its own texture and low emulsifying power. By coexisting with LP, the texture of soybean dietary fiber is relaxed, a smooth texture is realized, and emulsifying power is also imparted.
 本発明の粉末状大豆素材を使用する対象の食用組成物として具体的な応用例を示すと、例えば粉末状組成物、乳化組成物、焼きもしくは揚げ菓子、練り製品、組織状組成物、半固形状ないしゲル状組成物や、フライ食品用衣材(バッター)などに使用することにより、優れた物性改良効果を発揮することができる。
 また、本発明の粉末状大豆素材は、蛋白質と食物繊維に富むため、生理機能素材としても併用することができる。蛋白質又は食物繊維を強化することによって血中コレステロール低減、糖尿病性腎症予防、血糖上昇抑制、腸内環境改善等の栄養機能の発揮を目的にした、いわゆる機能性食品は、通常よりも蛋白質や繊維が多く含まれ、食用組成物の物性変化が起こりやすい。そのため上述の物性改良効果が発揮されるとより有効に作用するため、本発明の粉末状大豆素材を添加する対象として好ましい。
Specific application examples of the edible composition to which the powdered soybean material of the present invention is used include, for example, a powdered composition, an emulsified composition, a baked or fried confectionery, a kneaded product, a textured composition, a semisolid form In addition, by using it in a gel composition or a fried food material (batter), an excellent effect of improving physical properties can be exhibited.
Moreover, since the powdery soybean material of the present invention is rich in protein and dietary fiber, it can be used together as a physiologically functional material. By strengthening protein or dietary fiber, so-called functional foods aimed at demonstrating nutritional functions such as reducing blood cholesterol, preventing diabetic nephropathy, suppressing blood sugar rise, improving intestinal environment, etc. Many fibers are contained, and physical properties of the edible composition are likely to change. Therefore, when the above-described physical property improving effect is exerted, it works more effectively, so it is preferable as an object to which the powdered soybean material of the present invention is added.
(1-1)粉末状組成物
 粉末状組成物としては、例えば、粉末飲料、粉末スープ、粉末ソース、粉末味剤、粉末ピックル剤、ケーキ類・パン類・菓子類・麺類・フライ食品用衣材・惣菜(お好み焼きなど)等の小麦粉ミックス粉等が挙げられる。
 粉末状組成物へ本発明の粉末状大豆素材を使用した場合、これを水に分散させると分離大豆蛋白に比べてダマになりにくく、非常に分散性が良好となる。また、油分の高いスープや味剤などに使用した場合は乳化安定性にも優れるため、油染み等が生じにくくなる。さらに、即席ラーメン等の油分の高い味剤においては、味剤中の油分の酸化劣化も抑制することができ、酸化安定性にも寄与する。またフライ食品用衣材へ使用した場合、フライ食品にサクサク感を付与し、フライ後の油染みを抑制することができる。
(1-1) Powdered composition Examples of powdered compositions include powdered beverages, powdered soups, powdered sauces, powdered flavorings, powdered pickles, cakes, breads, confectionery, noodles, and fried food clothes. Examples include flour mixes such as ingredients and side dishes (okonomiyaki).
When the powdery soybean material of the present invention is used in a powdery composition, if it is dispersed in water, it is less likely to become lumpy than separated soybean protein, and the dispersibility is very good. In addition, when used in a soup or a flavoring agent having a high oil content, it is excellent in emulsification stability, so that oil stains are less likely to occur. In addition, in high-oil flavors such as instant ramen, the oxidative deterioration of the oil in the flavor can be suppressed, contributing to oxidation stability. Moreover, when it uses for the foodstuffs for fried foods, a crispy feeling can be provided to fried foods and the oil stain after frying can be suppressed.
(1-2)乳化組成物
 乳化組成物としては、例えば、マヨネーズ、ホイップクリーム、カスタードクリーム、フラワーペースト、チーズ類、ソース類等のO/W乳化組成物又はW/O乳化組成物が挙げられる。
 油分が比較的多く含まれる乳化組成物へ本発明の粉末状大豆素材を使用した場合、通常の濃縮大豆蛋白やおからなどを添加するよりも乳化性、保形性、粘性等に優れ、ネチャつきがなく滑らかでざらつかない等の食感改良効果を発揮する。該乳化組成物は、調理パン等のフィリングやトッピングとして利用した場合に加熱調理した際にも軟化してダレてしまったり、油分の分離が生じたりことが起こりにくく、耐熱保形性にも優れるものである。また野菜サラダ等に該乳化組成物をドレッシングとしてかけた場合にも水分の上昇によって乳化物がだれることを防止することができる。また、本発明の粉末状大豆素材はそれ自体にネチャツキやざらつきが少ないことから、乳化組成物の粘度調整剤としても利用することができる。
(1-2) Emulsified composition Examples of the emulsified composition include O / W emulsified compositions or W / O emulsified compositions such as mayonnaise, whipped cream, custard cream, flour paste, cheeses and sauces. .
When the powdered soybean material of the present invention is used in an emulsion composition that contains a relatively large amount of oil, it is superior in emulsifying properties, shape retention, viscosity, etc., compared to adding normal concentrated soybean protein or okara. It provides a texture-improving effect that is smooth and non-textured. The emulsified composition softens and sags even when cooked when used as a filling or topping for cooking pans, etc., and does not easily cause oil separation, and has excellent heat-resistant shape retention. Is. Further, when the emulsion composition is applied as a dressing to a vegetable salad or the like, the emulsion can be prevented from dripping due to an increase in moisture. Moreover, since the powdery soybean material of the present invention itself has little stickiness and roughness, it can also be used as a viscosity modifier for an emulsified composition.
(1-3)焼きもしくは揚げ菓子
 焼きもしくは揚げ菓子としては、例えば、ケーキ、クッキー、栄養バー、ビスケット、せんべい、かりんとう、あられ、スナック菓子、ドーナツ等が挙げられ、食感が良く食物繊維やLPの含有量が多く、低カロリーの物が得られる。非限定的には20重量%以上、好ましくは25重量%以上の食物繊維と、LCI値が60以上を示す大豆タンパク質を10重量%以上、好ましくは15重量%以上含有するもので、エネルギーが100g当たり500kcal以下、好ましくは400kcal以下のものが得られる。
1-3 High content and low calorie product. Non-limitatively containing 20% by weight or more, preferably 25% by weight or more of dietary fiber and soy protein having an LCI value of 60 or more, containing 10% by weight or more, preferably 15% by weight or more, and having an energy of 100 g Per kcal or less, preferably 400 kcal or less.
 MSPが多く含まれる既存の分離大豆蛋白等の大豆素材を使用した焼き菓子の生地では、水分が少ないと生地がまとまりにくく、生地がぼそぼそとして割れてしまう場合が多く、製造に熟練を要するのに比べて、焼き菓子の生地に本発明の粉末状大豆素材を利用した場合、該生地は硬くしまらずに適度な可塑性を有するため、生地の調製が極めて容易であり、焼成後には適度な硬さと軽く口溶けの良い食感を付与することができる。また保形性が高いため焼成後のパウンドケーキなどのエッジ部分の崩壊などを防ぐことができ、さらには焼成後の焼き型からの剥がれも良くなる。また、揚げ菓子については、吸油量を抑制したり、サクサク感を付与したりすることが可能である。 With existing baked confectionery doughs using soy materials such as isolated soy protein containing a lot of MSP, if the moisture is low, the dough is difficult to gather and the dough often breaks loosely and requires skill in production. In comparison, when the powdered soybean material of the present invention is used for the baked confectionery dough, the dough does not harden and has an appropriate plasticity, so that the preparation of the dough is extremely easy, and an appropriate hardness after baking. It can give a light and meltable mouthfeel. In addition, since the shape retention is high, it is possible to prevent the edge portion of the pound cake after the baking from collapsing, and the peeling from the baking mold after the baking is improved. Moreover, about fried confectionery, it is possible to suppress the amount of oil absorption or to give a crisp feeling.
 焼き菓子の一態様として、高食物繊維、高蛋白質、低カロリーのスナック菓子を調製する場合、例えば以下のように調製することができる。
 本発明の粉末状大豆素材に、小麦ふすま、ポテト繊維、難消化デキストリン、ポリデキストロース、イヌリンなどの食物繊維素材を組み合わせて配合し、これらを他の原料と共に混合して水と練り上げ、成型し、焼成する。これにより、乾燥固形分中50~95重量%を粉末状大豆素材と食物繊維素材が占める、口当たりの良い焼き菓子を調製することができる。また、摂取時の血糖上昇や脂肪蓄積を抑制させたい場合には、糖を無添加とする、あるいは糖アルコールなどに代え、また油脂も中鎖脂肪酸等に代えることができる。
 LPは蛋白質が脂質と会合している部分があり、7Sや11Sなどの貯蔵蛋白質に比べてプロテアーゼによる低分子化を受けにくいので消化が遅く腹持ちが良くなると考えられ、空腹感を抑制することでカロリーを抑えたダイエット補助食としても利用することができる。
As one aspect of the baked confectionery, when preparing a high dietary fiber, high protein, low calorie snack confectionery, for example, it can be prepared as follows.
The powdered soybean material of the present invention is combined with a combination of dietary fiber materials such as wheat bran, potato fiber, indigestible dextrin, polydextrose, inulin, etc., mixed with other ingredients, kneaded with water, molded, Bake. As a result, it is possible to prepare a baked confectionery having a good taste, in which the powdery soybean material and the dietary fiber material occupy 50 to 95% by weight in the dry solid content. In addition, when it is desired to suppress an increase in blood sugar and fat accumulation during ingestion, no sugar is added or sugar alcohol or the like can be used, and fats and oils can be replaced with medium chain fatty acids or the like.
LP has a part where protein is associated with lipid, and is less susceptible to low molecular weight by protease compared to storage proteins such as 7S and 11S. It can also be used as a dietary supplement with reduced calories.
(1-4)練り製品
 練り製品としては、例えば、ソーセージ、ハンバーグ、つくね、がんもどき、油揚げ、かまぼこ、ちくわ等が挙げられる。
 練り製品へ本発明の粉末状大豆素材を粉体として添加した場合には、生地への良好な分散性を示すので、ダマの形成が防止される。また、練り製品にゴム的でない、歯切れ及び喉通りが良い食感を付与することができる。またこれらの練り製品に対してレトルト加熱等の比較的厳しい熱ショックを与えても物性が変化しにくく、レトルト耐性が強い。
(1-4) Kneaded product Examples of the kneaded product include sausage, hamburger, tsukune, ganmodoki, fried chicken, kamaboko, chikuwa and the like.
When the powdery soybean material of the present invention is added to the kneaded product as a powder, it exhibits good dispersibility in the dough, so that formation of lumps is prevented. Further, the kneaded product can be given a texture that is not rubbery and has a good crispness and throat passage. Further, even if a relatively severe heat shock such as retort heating is applied to these kneaded products, the physical properties hardly change and the retort resistance is strong.
(1-5)半固形状ないしゲル状組成物
 半固形状ないしゲル状組成物としては、例えば、プリン、ゼリー、濃厚流動食、ヨーグルト状の大豆発酵食品等が挙げられる。
 半固形状ないしゲル状組成物へ本発明の粉末状大豆素材を使用した場合、従来の7Sや11Sのようなたわみのある強固なゲルとは異なり、軟弱なゲル状あるいは粘性の高い半固形状の物性となり、クリーミーで滑らかな食感を付与することができる。特に大豆発酵食品へ使用した場合、大豆由来の青臭み等も殆ど感じず良好な風味を実現できる。ヨーグルト状の大豆発酵食品において使用する蛋白質源としては、本発明の粉末状大豆素材を単独で使用しても良いし、豆乳や大豆粉の懸濁液を併用し、適宜所望の物性に調整することも可能である。
(1-5) Semi-solid or gel composition Examples of the semi-solid or gel composition include pudding, jelly, concentrated liquid food, yogurt-like fermented soybean food, and the like.
When the powdered soybean material of the present invention is used for a semi-solid or gel-like composition, unlike a conventional strong gel with flexibility such as 7S or 11S, a soft gel-like or highly viscous semi-solid It can provide a creamy and smooth texture. In particular, when used in fermented soybean foods, a good flavor can be realized with almost no blue odor derived from soybeans. As a protein source used in yoghurt-like fermented soybean food, the powdered soybean material of the present invention may be used alone, or a soymilk or soybean powder suspension is used in combination, and the desired physical properties are appropriately adjusted. It is also possible.
(2)機能性食品
 本発明の粉末状大豆素材は、上述のように様々な食用組成物に物性改良効果を付与できることに加え、食用組成物が本来備える風味や物性を損ねにくい生理機能素材としても利用することができる。
 該大豆素材は蛋白質と食物繊維に富み、さらには極性脂質としてリン脂質にも富むので、これらの成分を栄養的に強化するために用いることができる。特に該大豆素材中の主要な蛋白質であるLPは、大豆蛋白質の中で最もコレステロールの低減効果や糖尿病性腎症予防効果が認められる成分である。LPの摂取によってLPが胆汁酸と結合し、その排泄量が増えることから、アミノ酸源が腸まで届く可能性を考え合わせると、腸内の有用な有機酸を生産する腸内細菌にとって栄養源となる糖質や蛋白質を腸に届けることができると考えられる。さらに大豆食物繊維には、血糖上昇をゆるやかにする効果や腸内フローラを改善する効果、さらには抗酸化効果があると言われており、現代人の摂取量が少ないといわれる食物繊維を補うことおよびアンチエージングを目的にも使用ができる素材である。したがって血糖上昇抑制、腎臓保護、脂質代謝改善、整腸作用、ダイエットにも有効であると考えられ、生活習慣病予防のための食品素材として極めて利用価値が高いものである。
 したがって、粉末状大豆素材は、食用組成物の物性改良に利用されるのみでなく、これを食用組成物本来の物性を阻害しにくい蛋白質の強化素材や食物繊維の強化素材として利用し、これらの成分が強化された栄養的価値の高い食用組成物を製造することができる。またさらにはこれらの成分の強化により、血糖上昇抑制作用、腎臓保護作用、脂質代謝改善作用、整腸作用等の生理機能を発揮する機能性食品を製造することができる。機能性食品の種類は特に問わず、上記の(1-1)~(1-5)の各種食用組成物が例示される。ここで食用組成物において蛋白質や食物繊維を強化したというためには、少なくともこれらの成分が組成物中3重量%以上含まれることが好ましく、5重量%以上がより好ましい。
(2) Functional food The powdery soybean material of the present invention is a physiologically functional material that is difficult to impair the flavor and physical properties that the edible composition originally has, in addition to being able to impart physical properties improving effects to various edible compositions as described above. Can also be used.
The soy material is rich in protein and dietary fiber, and also rich in phospholipids as polar lipids, so it can be used to nutritionally strengthen these components. In particular, LP, which is a main protein in the soybean material, is a component in which the effect of reducing cholesterol and the effect of preventing diabetic nephropathy are recognized among soybean proteins. When LP is combined with bile acids by ingestion of LP and the amount of excretion increases, considering the possibility that an amino acid source can reach the intestine, it is a nutrient source for intestinal bacteria that produce useful organic acids in the intestine. Can be delivered to the intestine. In addition, soy dietary fiber is said to have an effect of easing blood sugar rise, improving intestinal flora, and antioxidant effect, and supplementing dietary fiber, which is said to be low in consumption by modern people It can also be used for anti-aging purposes. Therefore, it is considered to be effective for suppressing blood sugar elevation, protecting kidneys, improving lipid metabolism, intestinal regulation, and dieting, and is extremely useful as a food material for preventing lifestyle-related diseases.
Therefore, the powdered soybean material is not only used for improving the physical properties of the edible composition, but also used as a protein reinforcing material or a dietary fiber reinforcing material that does not obstruct the original physical properties of the edible composition. An edible composition with enhanced nutritional value can be produced. Furthermore, by strengthening these components, it is possible to produce a functional food that exhibits physiological functions such as an increase in blood sugar, a kidney protecting action, a lipid metabolism improving action, and an intestinal regulating action. The type of functional food is not particularly limited, and various edible compositions of the above (1-1) to (1-5) are exemplified. Here, in order to strengthen protein and dietary fiber in the edible composition, it is preferable that at least these components are contained in the composition in an amount of 3% by weight or more, and more preferably 5% by weight or more.
 従来の認識ではLPは大豆のオフフレーバーの原因と考えられており、これが高純度化されたものは必ずしも風味が良いものとは言えなかった。しかし、粉末状大豆素材においてはLPが大豆食物繊維と組み合せて含まれることで、意外にもLPによるオフフレーバーを感じにくく、風味が良好なものとなる。一方で大豆食物繊維も本来それ単独ではザラザラとした食感の悪いものであるが、LPと共存することで意外にも食感が滑らかでザラつきを感じ難いものとなる。したがって該大豆素材は上述の機能性食品に添加した際にLP又は大豆食物繊維の各欠点が影響を与えにくくなっており、物性や風味を損ねにくい機能性食品素材として極めて汎用性の高いものである。 According to the conventional recognition, LP is considered to be a cause of soybean off-flavor, and it has not always been said that the product with high purity has a good flavor. However, in the powdery soybean material, LP is contained in combination with soybean dietary fiber, so that it is surprisingly difficult to feel off-flavor due to LP, and the flavor is good. On the other hand, the soy dietary fiber originally has a poor texture when used alone, but the coexistence with LP makes the texture surprisingly smooth and difficult to feel. Therefore, when the soybean material is added to the above functional food, each defect of LP or soybean dietary fiber is less likely to be affected, and it is extremely versatile as a functional food material that does not easily impair physical properties and flavor. is there.
 以下に本発明の実施例を記載するが、本発明は、この実施例にのみ限定されるものではなく、本発明の要旨を逸脱しない範囲内において種々変更を加え得ることは勿論である。 EXAMPLES Examples of the present invention will be described below, but the present invention is not limited to these examples, and it goes without saying that various modifications can be made without departing from the scope of the present invention.
〔使用原料〕
 実施例及び比較例で用いる大豆素材A~Eは、以下の通り調製したもの又は市販品を用いた。
[Raw materials]
As the soybean materials A to E used in Examples and Comparative Examples, those prepared as follows or commercially available products were used.
・大豆素材A
 特許文献1の実施例5と同様の製法で、加工脱脂大豆を調製した。すなわち、密閉容器に充填した低変性脱脂大豆(PDI83、水分7.0%)1kgを相対湿度90%以上の雰囲気下で脱脂大豆の品温が85℃になるように湿熱加熱処理を行い、60分間維持した。容器から処理後の脱脂大豆を取り出し、加工脱脂大豆とした。この加工脱脂大豆のPDIは66であった。
 得られた加工脱脂大豆に水を15重量倍加え、pH7.0に調整し、25℃にて撹拌抽出を行った。得られた脱脂大豆スラリー(pH6.4)を遠心分離によって可溶性画分と不溶性画分とに分離した。この不溶性画分を回収し、不溶性画分の重量の3倍量の水を加えて加熱殺菌後、スプレードライヤーにて乾燥粉末化して大豆素材Aとした。
・ Soy material A
Processed defatted soybeans were prepared by the same production method as in Example 5 of Patent Document 1. That is, 1 kg of low-denatured defatted soybean (PDI83, moisture 7.0%) filled in an airtight container is subjected to wet heat treatment so that the product temperature of the defatted soybean is 85 ° C. in an atmosphere with a relative humidity of 90% or more and maintained for 60 minutes. did. The treated defatted soybean was taken out of the container and used as a processed defatted soybean. This processed defatted soybean had a PDI of 66.
Water was added to the obtained processed defatted soybean 15 times by weight, adjusted to pH 7.0, and extracted with stirring at 25 ° C. The obtained defatted soybean slurry (pH 6.4) was separated into a soluble fraction and an insoluble fraction by centrifugation. This insoluble fraction was collected, water three times the weight of the insoluble fraction was added, heat sterilized, and then dried and powdered with a spray dryer to obtain soybean material A.
・大豆素材B
 市販の濃縮大豆蛋白「アーコンS」(ADM社製)を用いた。
・ Soybean material B
Commercially available concentrated soy protein “Arcon S” (manufactured by ADM) was used.
・大豆素材C
  特許文献1の実施例5と同様の製法で、加工脱脂大豆を調製した。すなわち、密閉容器に充填した低変性脱脂大豆(PDI83、水分7.0%)1kgを相対湿度90%以上の雰囲気下で脱脂大豆の品温が85℃になるように湿熱加熱処理を行い、60分間維持した。容器から処理後の脱脂大豆を取り出し、加工脱脂大豆とした。この加工脱脂大豆のPDIは66であった。
 得られた加工脱脂大豆に水を15重量倍加え、pH6.0に調整し、25℃にて撹拌抽出を行った。得られた脱脂大豆スラリー(pH6.0)を遠心分離によって可溶性画分と不溶性画分とに分離した。この不溶性画分を回収し、不溶性画分の重量の3倍量の水を加えて加熱殺菌後、スプレードライヤーにて乾燥粉末化して大豆素材Cとした。
・ Soybean material C
Processed defatted soybeans were prepared by the same production method as in Example 5 of Patent Document 1. That is, 1 kg of low-denatured defatted soybean (PDI83, moisture 7.0%) filled in an airtight container is subjected to wet heat treatment so that the product temperature of the defatted soybean is 85 ° C. in an atmosphere with a relative humidity of 90% or more and maintained for 60 minutes. did. The treated defatted soybean was taken out of the container and used as a processed defatted soybean. This processed defatted soybean had a PDI of 66.
Water was added to the obtained processed defatted soybean 15 times by weight, adjusted to pH 6.0, and stirred and extracted at 25 ° C. The obtained defatted soybean slurry (pH 6.0) was separated into a soluble fraction and an insoluble fraction by centrifugation. This insoluble fraction was collected, water three times the weight of the insoluble fraction was added, heat sterilized, and then dried and powdered with a spray dryer to obtain soybean material C.
・大豆素材D
 市販の乾燥オカラ粉末「PF-20」((株)転生製)を用いた。
・ Soybean material D
A commercially available dry okara powder “PF-20” (manufactured by Tensei Co., Ltd.) was used.
・大豆素材E
 不二製油(株)製の乾燥オカラ粉末を用いた。
・ Soybean material E
Dry okara powder manufactured by Fuji Oil Co., Ltd. was used.
 得られた大豆素材A~Eについて、蛋白質含量(無脂固形分あたり)、食物繊維含量(無脂固形分あたり)、LCI値、MSP含量(蛋白質あたり)を測定し、その分析結果を表2に示した。なお、各測定方法は前記記載の方法で行った。 For the obtained soybean materials A to E, the protein content (per fat-free solid content), dietary fiber content (per fat-free solid content), LCI value, MSP content (per protein) were measured, and the analysis results are shown in Table 2. It was shown to. Each measurement method was performed by the method described above.
(表2)
Figure JPOXMLDOC01-appb-I000003
(Table 2)
Figure JPOXMLDOC01-appb-I000003
〔実施例1〕-粉末飲料―
 本発明の粉末状組成物の例として、粉末飲料を調製した。
 本発明の大豆素材A(蛋白質含量45%、LCI値70)を牛乳の中に混ぜてスプーンでかき混ぜた。分散が良く、ママ粉などの固い不溶な粉の塊はみられなかった。またオカラを含んでいるにもかかわらず、ざらつきが少なく滑らかな食感であった。
[Example 1]-Powdered beverage-
A powdered beverage was prepared as an example of the powdery composition of the present invention.
The soybean material A of the present invention (protein content 45%, LCI value 70) was mixed in milk and stirred with a spoon. Dispersion was good, and no solid insoluble powder lump such as mama powder was found. Despite containing okara, it had a smooth texture with little roughness.
〔比較例1〕
 大豆素材Aの代わりに大豆素材B(蛋白質含量70%、LCI値48)を用い、実施例1と同様に牛乳に混ぜてスプーンでかき混ぜた。分散が悪く、固いママコが生じた。
Comparative Example 1
Instead of soybean material A, soybean material B (protein content 70%, LCI value 48) was mixed with milk in the same manner as in Example 1 and stirred with a spoon. Dispersion was poor, and hard mako was produced.
〔比較例2〕
 大豆素材Aの代わりに大豆素材D(蛋白質含量25%、LCI値54)を用い、実施例1と同様に牛乳に混ぜてスプーンでかき混ぜた。分散は良かったが、ほとんどが沈み、溶解はせず、ひどくざらつきを感じた。
[Comparative Example 2]
Instead of soybean material A, soybean material D (protein content 25%, LCI value 54) was mixed with milk in the same manner as in Example 1 and stirred with a spoon. Dispersion was good, but most of it sank, did not dissolve and felt very rough.
〔実施例2〕-粉末状味剤-
 本発明の粉末状組成物の別の例として、粉末状味剤を調製した。
 本発明の大豆素材C(蛋白質含量60%、LCI値66)6部を、固形分20%で、その内に油を50%含むラーメンの味剤100部に加え、加熱混合して得た乳化物をスプレードライし、粉末状味剤を調製した。粉末として得られたものは油染みも少なく乳化安定性が高く、水に加えると程よく分散し、味も自然なものに感じられた。
[Example 2]-Powdery taste agent-
As another example of the powdery composition of the present invention, a powdery taste agent was prepared.
Emulsification obtained by adding 6 parts of soybean material C (protein content 60%, LCI value 66) of the present invention to 100 parts of a ramen flavoring agent having a solid content of 20% and containing 50% of oil, and mixing by heating. The product was spray-dried to prepare a powdery taste agent. The powder obtained had little oil stain and high emulsification stability, and when added to water, it was moderately dispersed and the taste felt natural.
〔比較例3〕
 大豆素材Cの代わりに大豆素材B(蛋白質含量70%、LCI値48)を用い、実施例2と同様の配合と処理方法にて調製した粉末状味剤は、油染みはないものの、水への分散が悪く、味もやや変化していた。
[Comparative Example 3]
The powdery taste agent prepared by the same composition and treatment method as in Example 2 using soybean material B (protein content 70%, LCI value 48) instead of soybean material C is not oil-stained. Dispersion was poor and taste was slightly changed.
〔比較例4〕
 大豆素材Cの代わりに大豆素材D(蛋白質含量25%、LCI値54)を用い、実施例2と同様の配合と処理方法にて粉末状味剤の調製を試みたが、原料を加熱混合しても乳化せず、噴霧が不可能であった。
 実施例2,比較例3,4の各味剤の評価を以下に示す。
[Comparative Example 4]
Instead of soybean material C, soybean material D (protein content 25%, LCI value 54) was tried to prepare a powdery flavoring agent by the same composition and processing method as in Example 2, but the ingredients were heated and mixed. However, it was not emulsified and spraying was impossible.
Evaluation of each taste agent of Example 2, Comparative Examples 3 and 4 is shown below.
(表3)
Figure JPOXMLDOC01-appb-I000004
(Table 3)
Figure JPOXMLDOC01-appb-I000004
〔実施例3〕-フライ食品用衣材への利用-
 本発明の粉末状組成物の別の例として、フライ食品用衣材を調製した。
 本発明の大豆素材A(蛋白質含量45%、LCI値70)を10部、薄力粉を90部を混合してフライ食品用衣材を調製した。これを水に溶いて粘度が1600mPa・s程度にしたものを油であげ、揚げたまとして食感を確認した。ほどよいサクサク感が感じられた。
[Example 3]-Application to fried food clothing-
As another example of the powdery composition of the present invention, a fried food apparel was prepared.
10 parts of soybean material A (protein content 45%, LCI value 70) of the present invention and 90 parts of soft flour were mixed to prepare a fried food apparel. This was dissolved in water to give a viscosity of about 1600 mPa · s with oil, and the texture was confirmed as fried. A moderate crispness was felt.
〔比較例5〕
 大豆素材Aの代わりに大豆素材B(蛋白質含量70%、LCI値48)を用い、実施例3と同じ配合でフライ食品用衣材を調製し、同様に評価したところ、実施例3に比べるとサクサク感が少なく、表面の硬さを感じた。
[Comparative Example 5]
When using the soybean material B (protein content 70%, LCI value 48) instead of the soybean material A, and preparing the foodstuffs for fried food with the same composition as Example 3, when evaluated similarly, compared with Example 3, There was little crispy feeling and felt the hardness of the surface.
〔比較例6〕
 大豆素材Aの代わりに大豆素材D(蛋白質含量25%、LCI値54)を用い、実施例3と同じ配合でフライ食品用衣材を調製し、同様に評価したところ、サクサク感は感じられたが崩れやすく、好ましくなかった。
[Comparative Example 6]
Using the soybean material D (protein content 25%, LCI value 54) instead of the soybean material A, and preparing a foodstuff for fried food with the same composition as Example 3, when evaluated in the same way, a crispy feeling was felt. Was easy to collapse and was not preferable.
〔実施例4〕-マヨネーズ-
 本発明の乳化組成物の例として、マヨネーズを調製した。
 本発明の大豆素材C(蛋白質含量60%、LCI値66)4部、水41部、大豆油50部、食酢5部をフードカッターに加えて5分撹拌してO/W乳化し、マヨネーズを調製した。該マヨネーズは滑らかで口の中で違和感なく溶けた。また、保形性を有し、加熱しても油が分離することなく、乳化安定性にも優れていた。
[Example 4] -Mayonnaise-
Mayonnaise was prepared as an example of the emulsified composition of the present invention.
4 parts of soybean material C (protein content 60%, LCI value 66) of the present invention, 41 parts of water, 50 parts of soybean oil and 5 parts of vinegar are added to a food cutter and stirred for 5 minutes to O / W emulsify, and mayonnaise Prepared. The mayonnaise was smooth and melted comfortably in the mouth. Moreover, it had shape retention property, and even when heated, the oil was not separated and the emulsion stability was excellent.
〔比較例7〕
 大豆素材Cの代わりに大豆素材B(蛋白質含量70%、LCI値48)を用い、実施例4と同様の配合と処理方法にて調製したマヨネーズの食感は、ややネチャつきがあり、重たい食感であった。保形性は無く、油分離が多かった。加熱するとやや凝集が起こり油の分離が進んだ。
[Comparative Example 7]
The texture of mayonnaise prepared using the same composition and processing method as in Example 4 using soy material B (protein content 70%, LCI value 48) instead of soy material C has a slightly sticky texture and is heavy. It was a feeling. There was no shape retention and there was much oil separation. When heated, a little flocculation occurred and oil separation progressed.
〔比較例8〕
 大豆素材Cの代わりに大豆素材D(蛋白質含量25%、LCI値54)を用い、実施例4と同様の配合と処理方法にてマヨネーズの調製を試みたが、原料が乳化せず、油が分離していた。
 実施例4、比較例7,8で調製した各マヨネーズの乳化の状態を表4に示す。
[Comparative Example 8]
Instead of soybean material C, soybean material D (protein content 25%, LCI value 54) was used to prepare mayonnaise with the same composition and treatment method as in Example 4, but the raw material was not emulsified and the oil It was separated.
Table 4 shows the state of emulsification of each mayonnaise prepared in Example 4 and Comparative Examples 7 and 8.
(表4)
Figure JPOXMLDOC01-appb-I000005
(Table 4)
Figure JPOXMLDOC01-appb-I000005
〔実施例5〕-バウンドケーキ-
 本発明の焼き菓子の第一例として、バウンドケーキを調製した。
 本発明の大豆素材A(蛋白質含量45%、LCI値70)20部にマーガリン20部、砂糖20部、卵白24部を混合し、焼成してバウンドケーキを得た。
[Example 5] -Bound cake-
As a first example of the baked confectionery of the present invention, a bound cake was prepared.
20 parts of soy material A (protein content 45%, LCI value 70) of the present invention was mixed with 20 parts of margarine, 20 parts of sugar and 24 parts of egg white, and baked to obtain a bound cake.
〔比較例9〕
 大豆素材Aを配合せず、小麦粉20部にマーガリン20部、砂糖20部、卵白24部を混合し、焼成してバウンドケーキを得た。
[Comparative Example 9]
Without blending soybean material A, 20 parts of wheat flour, 20 parts of margarine, 20 parts of sugar and 24 parts of egg white were mixed and baked to obtain a bound cake.
〔比較例10〕
 大豆素材Aの代わりに大豆素材B(蛋白質含量70%、LCI値48)20部にマーガリン20部、砂糖20部、卵白24部を混合し、焼成してバウンドケーキを得た。
[Comparative Example 10]
Instead of soybean material A, 20 parts of soybean material B (protein content 70%, LCI value 48) was mixed with 20 parts of margarine, 20 parts of sugar and 24 parts of egg white, and baked to obtain a bound cake.
〔比較例11〕
 大豆素材Aの代わりに大豆素材D(蛋白質含量25%、LCI値54)20部にマーガリン20部、砂糖20部、卵白24部を混合し、焼成してバウンドケーキを得た。
[Comparative Example 11]
Instead of soybean material A, 20 parts of soybean material D (protein content 25%, LCI value 54) was mixed with 20 parts of margarine, 20 parts of sugar and 24 parts of egg white, and baked to obtain a bound cake.
(表5)焼成後の形態と食感
Figure JPOXMLDOC01-appb-I000006
(Table 5) Form and texture after baking
Figure JPOXMLDOC01-appb-I000006
〔実施例6〕-せんべい-
 本発明の焼き菓子の第二例として、せんべいを調製した。
 本発明の大豆素材A(蛋白質含量45%、LCI値70)20部、マーガリン20部、砂糖15部を混合し、水を15部加えて混練りして生地を調製した。これを熱い鉄板で挟み、加熱してせんべいを調製した。
[Example 6] -Senbei-
As a second example of the baked confectionery of the present invention, rice crackers were prepared.
20 parts of soybean material A (protein content 45%, LCI value 70) of the present invention, 20 parts of margarine and 15 parts of sugar were mixed, and 15 parts of water was added and kneaded to prepare a dough. This was sandwiched between hot iron plates and heated to prepare a rice cracker.
〔比較例12〕
 大豆素材Aの代わりに大豆素材E(蛋白質含量40%、LCI値52)を用い、実施例6と同じ配合と調製方法でせんべいを調製した。
[Comparative Example 12]
A rice cracker was prepared by the same composition and preparation method as in Example 6 using soybean material E (protein content 40%, LCI value 52) instead of soybean material A.
〔比較例13〕
 大豆素材Aの代わりに大豆素材D(蛋白質含量25%、LCI値54)を用い、実施例6と同じ配合と調製方法でせんべいを調製した。
[Comparative Example 13]
Instead of soybean material A, soybean material D (protein content 25%, LCI value 54) was used to prepare rice crackers by the same composition and preparation method as in Example 6.
(表6)
Figure JPOXMLDOC01-appb-I000007
(Table 6)
Figure JPOXMLDOC01-appb-I000007
〔実施例7〕-レトルトソーセージ-
 本発明の練り製品の例として、レトルトソーセージを調製した。
 本発明の大豆素材A(蛋白質含量45%、LCI値70)100部に水400部、豚脂100部をニーダーに入れて混合し、充分に均一な生地にした後、でんぷん30部と香辛料などを含む味剤20部とを添加混合し、ケーシングに充填しレトルト加熱し、レトルトソーセージを得た。その食用組成物は、ほどよいソーセージ様の滑らかな喉通りの良い食感を呈した。
[Example 7] -Retort sausage-
Retort sausage was prepared as an example of the kneaded product of the present invention.
100 parts of soybean material A (protein content 45%, LCI value 70) of the present invention 400 parts of water and 100 parts of pork fat are mixed in a kneader to make a sufficiently uniform dough, then 30 parts of starch and spices, etc. 20 parts of a flavoring agent containing was added and mixed, filled into a casing and heated with retort to obtain a retort sausage. The edible composition exhibited a good sausage-like smooth throat texture.
〔比較例14〕
 大豆素材Aの代わりに大豆素材B(蛋白質含量70%、LCI値48)を用い、実施例7と同様の配合と処理方法にて調製したレトルトソーセージは、ややぱさつきがあり、なめらかでなく、喉通りの良くない食感をしていた。
 パネラー10名による官能評価で好ましい点数を10点満点で採点した。(×10点満点、普通が5点、悪いが0点で点数をつけ、)各調製品の官能評価項目で得られた点数の平均点を表7に示した。
[Comparative Example 14]
Retort sausage prepared using the same composition and processing method as in Example 7 using soy material B (protein content 70%, LCI value 48) instead of soy material A is somewhat crispy, not smooth, and throat I had a bad texture on the street.
In the sensory evaluation by 10 panelists, a favorable score was scored out of 10 points. Table 7 shows the average score obtained from the sensory evaluation items of each preparation (× 10 points, normal is 5 points, bad is 0 points).
(表7)
Figure JPOXMLDOC01-appb-I000008
(Table 7)
Figure JPOXMLDOC01-appb-I000008
〔実施例8〕-ヨーグルト状大豆発酵食品への利用-
 本発明の半固形状ないしゲル状組成物の例として、ヨーグルト状の大豆発酵食品を調製した。
 表8の配合の通り、水を60℃に加熱し、撹拌しながら大豆素材A(蛋白質含量45%、LCI値70)と乳酸菌以外の原材料を添加し、溶解後に高圧ホモゲナイザーを用いて圧力15MPaにて均質化した。その後90℃まで加熱後、43℃まで冷却した。次に、乳酸菌を添加し、カップに充填して蓋で密封し、43℃の恒温槽にてpHが4.5に低下するまでおよそ5時間発酵させ、その後10℃以下に冷却し、ヨーグルト状の大豆発酵食品を得た。
[Example 8] -Use for yogurt-like fermented soybean food-
As an example of the semi-solid or gel composition of the present invention, a yogurt-like fermented soybean food was prepared.
As shown in Table 8, water was heated to 60 ° C., and while stirring, soy material A (protein content 45%, LCI value 70) and raw materials other than lactic acid bacteria were added. After dissolution, the pressure was increased to 15 MPa using a high-pressure homogenizer. And homogenized. Thereafter, it was heated to 90 ° C. and then cooled to 43 ° C. Next, lactic acid bacteria are added, filled into a cup, sealed with a lid, fermented for about 5 hours in a thermostatic bath at 43 ° C. until the pH drops to 4.5, then cooled to below 10 ° C., and yogurt-like Of soy fermented food.
〔比較例15〕
 表8の通り、大豆素材Aの代わりに無調整豆乳(固形分9重量%、蛋白質含量4.5重量%、LCI値38)を配合する以外は実施例8と同様にしてヨーグルト状の大豆発酵食品を得た。
[Comparative Example 15]
As shown in Table 8, yoghurt-like soybean fermentation was carried out in the same manner as in Example 8 except that unadjusted soymilk (solid content 9% by weight, protein content 4.5% by weight, LCI value 38) was blended in place of soybean material A. Got food.
(表8)
Figure JPOXMLDOC01-appb-I000009
(Table 8)
Figure JPOXMLDOC01-appb-I000009
 実施例8の大豆発酵食品は、比較例15と比較して大豆食物繊維が含まれているにもかかわらず、大豆由来の青臭味等も殆どなく、7S、11Sの蛋白質成分が殆ど抜けているためか、クリーミーで滑らかな食感を持つヨーグルト状の物性であった。 Although the soybean fermented food of Example 8 contains soybean dietary fiber as compared with Comparative Example 15, there is almost no blue odor derived from soybean, and the protein components of 7S and 11S are almost lost. Because of this, it was a yogurt-like physical property with a creamy and smooth texture.
〔実施例9〕 -スナック風菓子-
 本発明の焼き菓子の第三例として、スナック風菓子を調製した。
 グラニュー糖10部にパーム油5部を混合し、大豆素材Aを25部、小麦ふすまを15部加えて混ぜ、水70部を加えて混練し、焼き菓子の生地を得た。生地を薄く延ばして成型し、160~170℃で30分焼成したところ、サクサクとしたスナック風の焼き菓子が得られた。本焼き菓子は大豆素材A由来の蛋白質が21%、総食物繊維が31%含まれ、エネルギーが100gあたり400kcalである、高蛋白質・高食物繊維・低カロリーの焼き菓子であった。
[Example 9] -Snack-style confectionery-
A snack-like confectionery was prepared as a third example of the baked confectionery of the present invention.
10 parts of granulated sugar was mixed with 5 parts of palm oil, 25 parts of soybean material A and 15 parts of wheat bran were added and mixed, and 70 parts of water was added and kneaded to obtain a baked confectionery dough. When the dough was thinly stretched and molded and baked at 160-170 ° C. for 30 minutes, a crisp snack-like baked confection was obtained. The baked confectionery was a high protein, high dietary fiber, low calorie baked confectionery that contained 21% protein derived from soybean material A, 31% total dietary fiber, and had an energy of 400 kcal per 100 g.
〔実施例10〕 -栄養バー(Nutrition bar)-
 本発明の焼き菓子の第四例として、栄養バーを調製した。
 マーガリン50部に、グラニュー糖30部、食塩0.2部、ガナッシュ27部を順次混合していき、次に大豆素材A63部、難消化性デキストリン33部、ココアパウダー1.5部、加工デンプン24部を予め混合したものを加え、レーズン45部、オレンジピール12部、乾燥パイン12部、スライスアーモンド12部をさらに加え、最後に全卵45部を加え、良く混合して生地を調製した。
 得られた生地を10cm×20cm×70cmの形状に成形し、170℃で22分焼成して栄養バーを得た。本焼き菓子は1本当たり76kcalで、蛋白質を約3%、食物繊維を約4%含むものであった。
[Example 10] -Nutrition bar-
As a fourth example of the baked confectionery of the present invention, a nutrition bar was prepared.
50 parts of margarine, 30 parts of granulated sugar, 0.2 part of salt, and 27 parts of ganache are sequentially mixed, then 63 parts of soybean material A, 33 parts of indigestible dextrin, 1.5 parts of cocoa powder, and modified starch 24 In addition, 45 parts of raisins, 12 parts of orange peel, 12 parts of dried pine, and 12 parts of sliced almonds were added, and 45 parts of whole eggs were finally added and mixed well to prepare a dough.
The obtained dough was formed into a shape of 10 cm × 20 cm × 70 cm and baked at 170 ° C. for 22 minutes to obtain a nutrition bar. This baked confectionery was 76 kcal per one, and contained about 3% protein and about 4% dietary fiber.
 本発明のLP主体の大豆蛋白質及び大豆食物繊維を主成分とする粉末状大豆素材を大豆素材として食用組成物に使用することにより、これまで分離大豆蛋白や濃縮大豆蛋白のような大豆素材では困難であった溶液および水分の少ないような練生地への速やかな分散性を付与したり、他にも保水性、保形性、乳化安定性や、違和感のない滑らかな食感を付与することができる。
 このような特徴を利用して、例えば流動性を有する食用組成物において粘度調整の効果はもちろん、液だれしやすい物を液だれしにくくする効果や、原料を混合した生地の成型性向上効果や、食用組成物の製品形態を崩れにくくする保形性向上効果や、油染みを抑える乳化安定化効果や、油脂の劣化を防止する酸化安定化効果を発揮することができる。
 また、本発明の粉末状大豆素材は、酸性条件下や、マグネシウムやカルシウムなどの蛋白質と相互作用しやすい2価金属イオン存在下においても、上記の食用組成物の物性改良効果が悪影響を受けにくいことも特長であり、栄養的に蛋白質と共にミネラルを強化した食用組成物を容易に製造することができる。
 したがって、さまざまな目的で食用組成物に利用することができ非常に汎用性に優れたものである。
 さらに、本発明の粉末状大豆素材自身がLPと食物繊維に富むため、生理機能が優れており、コレステロール低減作用、胆汁酸排泄作用、腎症予防作用、ダイエット作用、食物繊維による整腸作用、腸内フローラの改善作用などの目的でさまざまな食用組成物に利用することができる。
By using the powdered soybean material mainly composed of LP protein and soybean dietary fiber of the present invention as the edible composition, soy material such as separated soybean protein and concentrated soybean protein has been difficult so far. It can give quick dispersibility to the kneaded dough with less solution and moisture, and can also give water retention, shape retention, emulsification stability and smooth texture without any sense of incongruity it can.
Utilizing such characteristics, for example, in the edible composition having fluidity, not only the effect of adjusting the viscosity, but also the effect of making it difficult to spill the easily spilled material, the effect of improving the moldability of the dough mixed with the raw materials, In addition, it is possible to exhibit an effect of improving shape retention, which makes it difficult to disrupt the product form of the edible composition, an effect of stabilizing the emulsification that suppresses oil stains, and an effect of stabilizing the oxidation that prevents deterioration of fats and oils.
In addition, the powdered soybean material of the present invention is less susceptible to adverse effects on the physical property improvement effect of the above edible composition even under acidic conditions and in the presence of divalent metal ions that easily interact with proteins such as magnesium and calcium. This is also a feature, and it is possible to easily produce an edible composition nutritionally enriched with protein and minerals.
Therefore, it can be used for an edible composition for various purposes and is extremely versatile.
Furthermore, since the powdered soybean material of the present invention itself is rich in LP and dietary fiber, it has excellent physiological functions, cholesterol reduction action, bile acid excretion action, nephropathy prevention action, diet action, intestinal action by dietary fiber, It can be used in various edible compositions for the purpose of improving the intestinal flora.

Claims (7)

  1. 大豆蛋白質及び大豆食物繊維を含有し、蛋白質含量が無脂固形分重量あたり35~85重量%であって、蛋白質のLCI値が60以上であることを特徴とする粉末状大豆素材。 A powdered soy material comprising soy protein and soy dietary fiber, wherein the protein content is 35 to 85% by weight per non-fat solid content, and the LCI value of the protein is 60 or more.
  2. 大豆食物繊維含量が蛋白質に対して15~130重量%である、請求項1記載の粉末状大豆素材。 The powdered soy material according to claim 1, wherein the soy dietary fiber content is 15 to 130% by weight based on the protein.
  3. 請求項1記載の粉末状大豆素材を使用した食用組成物。 An edible composition using the powdered soybean material according to claim 1.
  4. 粉末状組成物、乳化組成物、焼きもしくは揚げ菓子、練り製品、又は半固形状ないしゲル状組成物である、請求項3記載の食用組成物。 The edible composition according to claim 3, which is a powdery composition, an emulsified composition, a baked or fried confectionery, a kneaded product, or a semi-solid or gel composition.
  5. 食用組成物が、蛋白質又は食物繊維を強化したものである、請求項4記載の食用組成物。 The edible composition according to claim 4, wherein the edible composition is a protein or dietary fiber reinforced.
  6. 大豆蛋白質及び大豆食物繊維を含有し、蛋白質含量が無脂固形分重量あたり35~85重量%であって、蛋白質のLCI値が60以上である粉末状大豆素材を食用組成物中に配合することを特徴とする食用組成物の物性改良方法。 A powdered soy material containing soy protein and soy dietary fiber, having a protein content of 35 to 85% by weight per non-fat solid content, and having an LCI value of protein of 60 or more is added to the edible composition. A method for improving the physical properties of an edible composition.
  7. 物性が分散性、保形性、保水性、乳化安定性、酸化安定性又は食感である請求項6記載の食用組成物の物性改良方法。 The method for improving physical properties of an edible composition according to claim 6, wherein the physical properties are dispersibility, shape retention, water retention, emulsion stability, oxidation stability or texture.
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