WO2012083572A1 - Flocking swab for biological sampling quantitatively and method for making the same - Google Patents
Flocking swab for biological sampling quantitatively and method for making the same Download PDFInfo
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- WO2012083572A1 WO2012083572A1 PCT/CN2011/000203 CN2011000203W WO2012083572A1 WO 2012083572 A1 WO2012083572 A1 WO 2012083572A1 CN 2011000203 W CN2011000203 W CN 2011000203W WO 2012083572 A1 WO2012083572 A1 WO 2012083572A1
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- swab
- flocking
- fiber
- sampling
- ring
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N2001/028—Sampling from a surface, swabbing, vaporising
Definitions
- the present invention relates to a biological experimental apparatus and a method of manufacturing the same, and more particularly to a quantitative biological sampling flocking swab and a method of manufacturing the same.
- sampling is one of the most common and convenient sampling techniques, with sampling swabs being the most critical sampling equipment.
- the sampling transfer system consists of sampling swabs to complete the process of reliably collecting biological samples from the sampling site and safely transferring them to the laboratory.
- the swab rod is a rod (generally cylindrical) made of a material having a certain hardness and elasticity.
- One end is a hand-held part, and one end or both ends can be used as a part for making a swab head.
- the swab head is a portion that is fixed at one or both ends of the swab rod to effectively absorb the biological sample, and is generally bud-like.
- the swab head is divided into three categories: long fiber winding (wound swab), short fiber flocking (flocking swab) and polyurethane foaming (foaming swab).
- the entangled swab is made by a fiber material winding process, and the flocking swab is made by a short flocking fiber material by an electrostatic flocking process, the fiber is generally a natural fiber, a chemical fiber and a mixture of these fibers; the foaming swab uses a polyurethane And the foaming agent is produced by a foaming process.
- the wrap swab is the first sampling device used for swab sampling technology, because it is difficult to control the raw Process factors such as bond material, fiber length, fiber denier, and tightness of the entanglement result in significant differences in sampling performance.
- Subsequent flocking and foaming swabs overcome some of the defects of the wrapped swab, and the sampling performance is relatively stable, especially for the adsorption and release properties of the sample.
- the main mechanisms for the adsorption of biological samples by the three types of swabs are fiber surface adsorption (winding and flocking swabs), micropore surface adhesion (foaming swabs) and capillary effects (flocking and foaming swabs).
- the three types of biological sampling swabs still have the following inherent defects during the sampling process:
- the amount of sample adsorbed by flocking and foaming swabs is lower than that of wound swabs.
- the diameter of the swab is limited. If it is too thin, the sampling swab cannot be made.
- the flocking swab is flocked at the top of the swab, and the effective flocking area is small. If the swab is too small, the swab can not be flocked; the swab head formed by the foaming material is loose, and the swab diameter is too small. Fall off. 3 ⁇ 4
- the flocking and foaming swab heads are distributed along the long axis of the swab rod at one end of the swab rod.
- the effective contact portion of the swab head and the sampling portion is less than half of the whole swab head, which is often ineffective. Collect liquid samples.
- the tip (head) of the three types of swabs may damage the sampling site, such as human organs.
- the present invention also provides a method of manufacturing the quantitative biological sample flocking swab.
- the present invention provides a quantitative biological sampling flocking swab comprising a swab rod and a swab head, and the end of one or both ends of the swab rod is arranged as an annular bracket structure, and the flock layer is covered The surface of the annular stent forms a swab head.
- the effective flocking area of the flocking substrate is set to 2. 5 ⁇ 296 legs 2 .
- the annular bracket is arranged in a circular ring shape, an elliptical ring shape, a three-sided ring shape or a polygonal ring shape of at least three sides.
- the annular bracket cylinder has a circular cross section, an elliptical shape or a polygonal shape.
- the material of the swab rod is polypropylene plastic, polyethylene plastic, polybutylene plastic, polystyrene plastic or engineering plastic.
- the velvet layer is covered with natural fibers, or chemical fibers, or a mixture of the two on the surface of the annular stent.
- the fiber density of the flock layer is 1. 5 ⁇ 350 ⁇ g/ ⁇ 2 ⁇
- the fiber density of the flock layer is 1. 5 ⁇ 350 ⁇ g / mm 2 .
- the flock layer is covered with nylon fibers on the surface of the annular stent.
- the swab bar is provided with a break port for facilitating the breakage of the swab bar, or a swab having a modified polystyrene tanning material that can be broken at any point without a break.
- the manufacturing method of the present invention comprises the production of a swab rod and a swab head, and specifically includes the following steps: forming a swab rod by an injection molding process One end or both ends are in the form of a ring-shaped bracket; the ring-shaped bracket is used as a flocking substrate, and the surface of the ring-shaped bracket substrate is covered with a conventional electrostatic flocking process to form a swab head, and the flocking layer is formed.
- the material is made of natural fiber, or chemical fiber, or a mixture of the two; by calculating the effective flocking area of the annular stent, the inner diameter of the annular stent is designed as
- the annular stent cylinder cross-sectional diameter is designed to be 0. 2 ⁇ 3 mm, to achieve control of the effective flocking area' value between 2. 5 ⁇ 296 mm 2 range, finally obtained sampling Quantitative biological sampling of flocked swabs with a range of values between ⁇ and 200 ⁇ .
- the density of the flocked flocking fibers is controlled to 1. 5 ⁇ 350 ⁇ .
- the density of the flocked flocking fibers is controlled to 1. 5 ⁇ 350 ⁇ . g/mm 2 .
- the material of the flock layer (2) is made of nylon fiber.
- the inner diameter of the annular stent and the cross-sectional diameter of the annular stent cylinder for quantitative biosampling flocking swabs are the main factors determining the effective flocking area and controlling the volume of sample collection and quantitative sampling.
- the volume of the sample that the swab can collect is also affected by the fiber length, fiber denier, and fiber density of the flock layer.
- the flocking surface area of the substrate obtained by using the annular stent as the substrate is increased, that is, the effective sampling amount is increased by increasing the effective fluff area.
- the invention controls the surface area of the flocking substrate by controlling the inner ring diameter of the annular support and the cross-sectional diameter of the annular support cylinder, and further adjusts the fiber fineness, fiber length and fiber density of the flock layer, and finally wipes
- the subhead has a quantitative sampling function. Swab made according to the principles of the present invention,
- the controllable quantitative sampling volume detected by the water absorption gravimetric analysis method (H 2 0@25 ° C) at 25 ° C is a fixed value in the range of 10 to 200 ⁇ 1 .
- the effective sampling part of the flocked sampling swab manufactured by the invention is concentrated on the end of the swab rod, it can be completely contacted with the sampled part during sampling, so as to ensure sufficient sample collection;
- the simple cylindrical design of the end of the swab rod for many years makes the swab head difficult to fall off and effectively eliminates the possibility of sampling swabs causing damage to the sampling site.
- the quantitative biological sampling flocking swab obtained by the invention and the manufacturing method thereof have the following advantages, and
- Quantitative biological sampling flocking swabs with different sampling quantities can be selected according to the needs of collecting samples.
- Quantitative biological sampling flocking swabs can quantitatively collect samples, which can help to directly calculate the results after experimental testing.
- Quantitative biological sampling flocking swabs effectively solve the problem of loosening and shedding of the swab head.
- Fig. 1 is a cross-sectional view showing the structure of a first embodiment of the present invention.
- 2 is a schematic cross-sectional view of a cylindrical stent cylinder according to Embodiment 1 of the present invention.
- Fig. 3 is a cross-sectional view showing a ring-shaped bracket according to a first embodiment of the present invention.
- FIG. 4 is a cross-sectional view showing the structure of a swab produced in the prior art.
- 1-swab rod 2-flock layer
- 3-fracture 4-ring bracket
- this embodiment provides a quantitative biological sampling flocking swab and a manufacturing method thereof, including a swab rod 1 and a swab head, and one end of the swab rod 1
- the ends of the annular holders 4 are arranged in a ring shape by a conventional injection molding process, or the ends of the ends of the swabs 1 can be respectively arranged as an annular support 4.
- the annular bracket 4 may be arranged in an elliptical ring shape, a three-sided ring shape or a polygonal ring shape of at least three sides; the annular bracket 4 has a cylindrical cross section which is arranged in a circular shape, or may be arranged in an elliptical shape or a polygonal shape.
- the embodiment is set to 1.19 mm; the swab rod 1 and the annular bracket 4 may be made of polyethylene plastic, polyterephthalic plastic or engineering plastic. In this embodiment, polypropylene plastic is used.
- a baffle 3 for facilitating the breakage of the swab rod 1 is provided in the swab rod 1 to facilitate the transfer after sampling; or a swab made of modified polystyrene material can also be used.
- Rod 1 is achieved to break at any point.
- the annular scaffold 4 is used as a base material, and the flock layer 2 is covered on the surface of the base material of the annular support 4 by a conventional electrostatic flocking process, and the effective flocking layer area of the surface of the annular support 4 substrate can be set at 5 to 296 mm.
- this embodiment is 46 mm 2 ; the fiber length of the flock layer 2 can be set between 0.2 and 3.0 mm, which is 0.6 mm in this embodiment; the fiber fineness of the flock layer 2 can be set at 0.33 ⁇ Between 7.0 dtex range, this embodiment is 1.5 dtex; the fiber density of the flock layer 2 can be set between 1.5 and 350 g/mm 2 , which is 17 ⁇ g/mm 2 in this embodiment; swab head flocking
- the material involved in layer 2 is a natural fiber, or a chemical fiber, or a mixed fiber of two, which is a nylon fiber in this embodiment.
- the advantage of this embodiment is that the manufacturing process is simple, the amount of the sample is large, and the volume of the liquid to be quantitatively adsorbed is determined to be 55 ⁇ ⁇ (0@25 ° C), and the swab head is not easy. Shedding, easy to use and safe.
- the annular support 4 has an inner ring diameter of 5 mm, the annular support 4 has a cylindrical cross-sectional diameter of 1.5 mm, the annular support 4 has an effective flocking area of 74 mm 2 , and the nylon fiber has a length of 1.5 ran, and the fiber has a fineness. 6.67 dtex, the fiber density of the flock layer 2 is 328 ⁇ g/mm 2 , and the volume of the adsorbed liquid is 110 ⁇ ⁇ (H 2 0@25 ° C), and the rest is the same as in the first embodiment.
- the inner ring of the annular bracket 4 has a diameter of 1.5 mm
- the annular bracket 4 has a cross-sectional diameter of 0.5 mm
- the effective flocking area of the substrate of the annular bracket 4 is 7.39 mm 2
- the length of the nylon fiber is 1.0 mm
- the fiber density of the flock layer 2 is 109 ⁇ g/mm 2
- the volume of the adsorbed liquid is 20 ⁇ ⁇ (25 ° C), and the rest is the same as in the first embodiment.
Abstract
The invention relates to a flocking swab for biological sampling quantitatively and a method for making the same. One or two terminals of the rod form a loop frame by injection molding process. A flocking layer locates on the surface of the loop frame by conventional electrostatic flocking process. By controlling the diameters of the loop frame and the cylinder of the loop frame and controlling the key flocking parameters, the swab for biological sampling is obtained, which can adsorb liquid samples quantitatively. It accomplishes the purposes of quantitative sampling, large sampling volume, hardly falling off for the head of the swab, easy to use and good security.
Description
技术领域 Technical field
本发明涉及一种生物学实验器材及其制造方法，尤其是涉及一种 定量生物采样植绒拭子及其制造方法。 The present invention relates to a biological experimental apparatus and a method of manufacturing the same, and more particularly to a quantitative biological sampling flocking swab and a method of manufacturing the same.
背景技术 Background technique
在生物学领域， 生物样本（包括生物细胞和生物分子）的采集和 传递是能否进行有效实验室检査的首要条件。在众多生物样本采集和 传递技术中， 拭子采样是一种最常用和最便捷的采样技术， 其中采样 拭子是最关键的采样器材。围绕采样拭子组成采样传递系统器材可以 完成由采样部位可靠采集生物样本和安全传递到实验室的全部过程。 In the field of biology, the collection and transfer of biological samples, including biological cells and biomolecules, is a prerequisite for effective laboratory testing. Among the many biological sample collection and transfer technologies, swab sampling is one of the most common and convenient sampling techniques, with sampling swabs being the most critical sampling equipment. The sampling transfer system consists of sampling swabs to complete the process of reliably collecting biological samples from the sampling site and safely transferring them to the laboratory.
目前， 传统生物采样拭子由拭子杆和拭子头组成。拭子杆为一个 棒状物， （一般为圆柱状)， 由有一定硬度并有弹性的材料制作， 一端 为手持部位， 一端或二端均可作为制作拭子头的部位。拭子头为固定 在拭子杆一端或二端的能够有效吸附生物样本的部分， 一般为蓓蕾 状。 按照制作材料和工艺， 拭子头分为长纤维缠绕 （缠绕拭子）、 短 纤维植绒（植绒拭子） 以及聚氨酯发泡（发泡拭子）三大类。 缠绕拭 子采用纤维材料经缠绕工艺制作，植绒拭子采用短绒纤维材料经静电 植绒工艺制作， 所述纤维一般为天然纤维、化学纤维以及这些纤维的 混合物； 发泡拭子则使用聚胺酯及发泡剂经发泡工艺制作。 Currently, traditional biological sampling swabs consist of a swab shaft and a swab head. The swab rod is a rod (generally cylindrical) made of a material having a certain hardness and elasticity. One end is a hand-held part, and one end or both ends can be used as a part for making a swab head. The swab head is a portion that is fixed at one or both ends of the swab rod to effectively absorb the biological sample, and is generally bud-like. According to the materials and processes, the swab head is divided into three categories: long fiber winding (wound swab), short fiber flocking (flocking swab) and polyurethane foaming (foaming swab). The entangled swab is made by a fiber material winding process, and the flocking swab is made by a short flocking fiber material by an electrostatic flocking process, the fiber is generally a natural fiber, a chemical fiber and a mixture of these fibers; the foaming swab uses a polyurethane And the foaming agent is produced by a foaming process.
缠绕拭子是最先用于拭子采样技术的采样器材，由于难以控制生
产工艺要素（如粘结材料、纤维长度、纤维纤度以及缠绕的紧密度等） 导致其采样性能出现明显差异。之后出现的植绒和发泡拭子克服了缠 绕拭子的部分缺陷， 采样性能相对稳定， 尤其对样本的吸附和释放性 能显著提高。三类拭子头吸附生物样本的主要机制分别是纤维表面吸 附（缠绕和植绒拭子）、微孔表面粘附（发泡拭子）和毛细管效应（植 绒和发泡拭子）。 综合比较， 三类生物采样拭子在采样过程中仍然存 在如下固有缺陷： The wrap swab is the first sampling device used for swab sampling technology, because it is difficult to control the raw Process factors such as bond material, fiber length, fiber denier, and tightness of the entanglement result in significant differences in sampling performance. Subsequent flocking and foaming swabs overcome some of the defects of the wrapped swab, and the sampling performance is relatively stable, especially for the adsorption and release properties of the sample. The main mechanisms for the adsorption of biological samples by the three types of swabs are fiber surface adsorption (winding and flocking swabs), micropore surface adhesion (foaming swabs) and capillary effects (flocking and foaming swabs). Comprehensive comparison, the three types of biological sampling swabs still have the following inherent defects during the sampling process:
( 1 ) 植绒和发泡拭子的样本吸附量比缠绕拭子低。 (1) The amount of sample adsorbed by flocking and foaming swabs is lower than that of wound swabs.
( 2 ) 拭子杆直径受限制， 过细即不能制作采样拭子。 植绒拭子 在拭子杆顶端植绒， 有效植绒面积小， 拭子杆直径过小即不能植绒； 通过发泡材料包裹作用形成的拭子头比较松动，拭子杆直径过小容易 脱落。 ¾ (2) The diameter of the swab is limited. If it is too thin, the sampling swab cannot be made. The flocking swab is flocked at the top of the swab, and the effective flocking area is small. If the swab is too small, the swab can not be flocked; the swab head formed by the foaming material is loose, and the swab diameter is too small. Fall off. 3⁄4
( 3 ) 植绒和发泡拭子头均沿拭子杆长轴分布于拭子杆一端， 在 实际使用时拭子头与采样部位的有效接触部分不足拭子头整体的一 半， 往往不能有效采集液体样本。 (3) The flocking and foaming swab heads are distributed along the long axis of the swab rod at one end of the swab rod. In actual use, the effective contact portion of the swab head and the sampling portion is less than half of the whole swab head, which is often ineffective. Collect liquid samples.
(4)已有三类拭子的制作技术均只能获得不定量样本采集拭子， 不具备定量采集生物样本的性能。 至今尚无可定量采样的拭子。 (4) There are three types of swabs that can only obtain unquantified sample collection swabs, and do not have the ability to quantitatively collect biological samples. There are no swabs that can be quantified so far.
( 5 ) 三类拭子杆的尖端 （头部） 有可能损伤采样部位， 如人体 器官。 i 发明内容
量低， 以及解决拭子头易脱落和不安全的问题； 为此， 本发明还提供 一种制造该定量生物采样植绒拭子的方法。 (5) The tip (head) of the three types of swabs may damage the sampling site, such as human organs. i Summary of the invention The amount is low, and the problem that the swab head is easy to fall off and unsafe is solved; therefore, the present invention also provides a method of manufacturing the quantitative biological sample flocking swab.
为了实现上述目的， 本发明提供一种定量生物采样植绒拭子， 包 括拭子杆和拭子头，将拭子杆的一端或两端的端部设置为环状支架结 构， 植绒层覆盖在环状支架的表面形成拭子头。 In order to achieve the above object, the present invention provides a quantitative biological sampling flocking swab comprising a swab rod and a swab head, and the end of one or both ends of the swab rod is arranged as an annular bracket structure, and the flock layer is covered The surface of the annular stent forms a swab head.
环状支架内环直径为 1. 25〜10 mm, 环状支架柱体横截面直径为 0. 2〜3 mm;或者环状支架作为植绒基材的有效植绒面积设置为 2. 5〜 296 腿 2。 5〜 The effective flocking area of the flocking substrate is set to 2. 5~ 296 legs 2 .
环状支架设置成圆环形、椭圆环形、三边环形或至少三边以上的 多边环形， 环状支架柱体横截面设置成圆形、 椭圆形或多边形。 The annular bracket is arranged in a circular ring shape, an elliptical ring shape, a three-sided ring shape or a polygonal ring shape of at least three sides. The annular bracket cylinder has a circular cross section, an elliptical shape or a polygonal shape.
拭子杆的材料为聚丙烯塑料、聚乙烯塑料、聚对苯二甲酸类塑料、 聚苯乙烯塑料或工程塑料。 The material of the swab rod is polypropylene plastic, polyethylene plastic, polybutylene plastic, polystyrene plastic or engineering plastic.
^絨层采用天然纤维， 或者化学纤维， 或者二者的混合纤维覆盖 在环状支架的表面。 The velvet layer is covered with natural fibers, or chemical fibers, or a mixture of the two on the surface of the annular stent.
植绒层的纤维长度为 0. 2〜3. 0 mm, 植绒层的纤维纤度为 0. 33〜 7. 0 dtex, 植绒层的纤维密度为 1. 5〜350 μ g/mm2。 5〜350 μ g/毫米 2。 The fiber density of the flock layer is 1. 5~350 μ g / mm 2 . The fiber density of the flock layer is 1. 5~350 μ g / mm 2 .
植絨层采用尼龙纤维覆盖在环状支架的表面。 The flock layer is covered with nylon fibers on the surface of the annular stent.
拭子杆上设置有便于定点折断拭子杆的折断口，或用改性的聚苯 乙烯孿料制作的能任意点折断的拭子杆上不设折断口。 The swab bar is provided with a break port for facilitating the breakage of the swab bar, or a swab having a modified polystyrene tanning material that can be broken at any point without a break.
为了制造定量生物采样植绒拭子，本发明的制造方法包括拭子杆 和拭子头的制作， 具体包括以下步骤， 通过注塑工艺将拭子杆制作成
一端或两端呈环状支架结构； 再以环状支架作为植绒基材， 在环状支 架基材的表面用常规静电植絨工艺覆盖植绒层后制成拭子头，植绒层 的材料采用天然纤维， 或者化学纤维， 或者二者的混合纤维制作； 通 过计算改变环状支架的有效植绒面积， 将环状支架内环直径设计为In order to manufacture a quantitative biological sampling flocking swab, the manufacturing method of the present invention comprises the production of a swab rod and a swab head, and specifically includes the following steps: forming a swab rod by an injection molding process One end or both ends are in the form of a ring-shaped bracket; the ring-shaped bracket is used as a flocking substrate, and the surface of the ring-shaped bracket substrate is covered with a conventional electrostatic flocking process to form a swab head, and the flocking layer is formed. The material is made of natural fiber, or chemical fiber, or a mixture of the two; by calculating the effective flocking area of the annular stent, the inner diameter of the annular stent is designed as
1. 25〜10 mm, 将环状支架柱体横截面直径设计成 0. 2〜3 mm, 来实 现控制有效植绒面积'的值在 2. 5〜296 mm2范围之间，最终得到采样量 能在 ^〜200 μ ΐ范围之间选择的定量生物采样植绒拭子。 1. 25〜10毫米, The annular stent cylinder cross-sectional diameter is designed to be 0. 2~3 mm, to achieve control of the effective flocking area' value between 2. 5~296 mm 2 range, finally obtained sampling Quantitative biological sampling of flocked swabs with a range of values between ^ and 200 μΐ.
综合控制拭子头的环状支架结构和植绒密度实现拭子头定量采 集液体样本， 在 25°C条件下重量分析法测得的拭子头吸附去离子水 的体积范围为在 10〜200 μ ΐ之间的确定值。 Comprehensive control of the annular stent structure and flocking density of the swab head enables quantitative sampling of the liquid sample by the swab head. The volume of the deionized water adsorbed by the swab head measured by gravimetric analysis at 25 ° C is in the range of 10 to 200. The determined value between μ ΐ.
将植绒层纤维长度控制为 0. 2〜3. 0 mm, 将植绒层纤维纤度控制 为 0. 33〜7. 0 dtex，将植绒层植绒纤维密度控制为 1. 5〜350 μ g/mm2。 5〜350 μ The density of the flocked flocking fibers is controlled to 1. 5~350 μ. The density of the flocked flocking fibers is controlled to 1. 5~350 μ. g/mm 2 .
植絨层 （2 ) 的材料采用尼龙纤维。 The material of the flock layer (2) is made of nylon fiber.
定量生物采样植绒拭子的环状支架内环直径和环状支架柱体横 截面直径是决定有效植绒面积进而控制采集样品体积和实现定量采 集样本的主要因素。此外， 拭子能够采集样本的体积还受植绒层的纤 维长度、纤维纤度以及纤维密度的影响。与普通端部为圆柱状的传统 拭子头相比， 以环状支架为基材所获得的基材的植绒表面积增加， 也 就是通过增加有效 绒面积增大了有效采样量。本发明通过控制环状 支架内环直径和环状支架柱体横截面直径，控制实施植绒基材的表面 积， 迸一步调节植绒层的纤维纤度、纤维长度和纤维密度的变化， 最 终使得拭子头具备定量采样功能。按照本发明的原理制作的拭子， 经
25°C 境条件下吸水重量分析法（H20@25°C )检测到的可控制定量采 样体积为 10〜200 μ 1范围的一个定值。 The inner diameter of the annular stent and the cross-sectional diameter of the annular stent cylinder for quantitative biosampling flocking swabs are the main factors determining the effective flocking area and controlling the volume of sample collection and quantitative sampling. In addition, the volume of the sample that the swab can collect is also affected by the fiber length, fiber denier, and fiber density of the flock layer. Compared with the conventional swab head having a cylindrical end, the flocking surface area of the substrate obtained by using the annular stent as the substrate is increased, that is, the effective sampling amount is increased by increasing the effective fluff area. The invention controls the surface area of the flocking substrate by controlling the inner ring diameter of the annular support and the cross-sectional diameter of the annular support cylinder, and further adjusts the fiber fineness, fiber length and fiber density of the flock layer, and finally wipes The subhead has a quantitative sampling function. Swab made according to the principles of the present invention, The controllable quantitative sampling volume detected by the water absorption gravimetric analysis method (H 2 0@25 ° C) at 25 ° C is a fixed value in the range of 10 to 200 μ 1 .
由于本发明制造的植绒采样拭子的有效采样部位集中于拭子杆 端部，在实施采样时可以与被采样部位完全接触，保证充分采集样本; 由于采用环状支架结构，不再采用沿袭多年的拭子杆端部的简单柱状 设计，使拭子头既不易脱落也有效消除了采样拭子造成采样部位伤害 的可能性。 ' Since the effective sampling part of the flocked sampling swab manufactured by the invention is concentrated on the end of the swab rod, it can be completely contacted with the sampled part during sampling, so as to ensure sufficient sample collection; The simple cylindrical design of the end of the swab rod for many years makes the swab head difficult to fall off and effectively eliminates the possibility of sampling swabs causing damage to the sampling site. '
本发明获得的定量生物采样植绒拭子及其制造方法具有以下优 占、、. · The quantitative biological sampling flocking swab obtained by the invention and the manufacturing method thereof have the following advantages, and
( 1 )能够根据采集样本的需要选择制造不同采样量的定量生物采 样植绒拭子。 (1) Quantitative biological sampling flocking swabs with different sampling quantities can be selected according to the needs of collecting samples.
(2)定量生物采样植绒拭子的拭子头有效部位与采样部位完全接 触， 保证采样量最大化。 (2) The effective part of the swab head of the quantitative biological sampling flocking swab is completely in contact with the sampling part to ensure the maximum sampling amount.
(3)定量生物采样植绒拭子能够定量采集样本， 有助于实现实验 检测后直接计算结果。 (3) Quantitative biological sampling flocking swabs can quantitatively collect samples, which can help to directly calculate the results after experimental testing.
( 4) 定量生物采样植绒拭子有效地解决了拭子头松动和脱落问 题。 (4) Quantitative biological sampling flocking swabs effectively solve the problem of loosening and shedding of the swab head.
( 5 )定量生物采样植绒拭子完全克服了拭子对采样部位 (如人体） 造成伤害的问题。 附图说明 (5) Quantitative biological sampling flocking swabs completely overcome the problem of swabs causing damage to the sampling site (such as the human body). DRAWINGS
图 1为本发明实施例一的结构剖视图。
图 2为本发明实施例一的环状支架柱体横截面示意图。 Fig. 1 is a cross-sectional view showing the structure of a first embodiment of the present invention. 2 is a schematic cross-sectional view of a cylindrical stent cylinder according to Embodiment 1 of the present invention.
图 3为本发明实施例一的环状支架的剖视图。 Fig. 3 is a cross-sectional view showing a ring-shaped bracket according to a first embodiment of the present invention.
图 4为现有技术生产的拭子结构剖视图。 4 is a cross-sectional view showing the structure of a swab produced in the prior art.
其中： 1-拭子杆， 2-植絨层， 3-折断口， 4-环状支架。 Among them: 1-swab rod, 2-flock layer, 3-fracture, 4-ring bracket.
具体实施方式 detailed description
下面根据附图详细说明本发明的优选实施例。 Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
施例一如图 1、 图 2、 图 3所示， 本实施例提供一种定量生物 采样植绒拭子及其制造方法， 包括拭子杆 1和拭子头， 将拭子杆 1的 一端的端部通过常规注塑工艺设置成环状支架 4， 或者也可以将拭子 杆 1两端的端部分别设置成环状支架 4， 本实施例的环状支架 4的环 状设置成圆环形， 或者也可以设置成椭圆环形、三边环形或至少三边 以上的多边环形； 环状支架 4柱体横截面设置成圆形， 或者也可以设 置成椭圆形或多边 。 As shown in FIG. 1, FIG. 2 and FIG. 3, this embodiment provides a quantitative biological sampling flocking swab and a manufacturing method thereof, including a swab rod 1 and a swab head, and one end of the swab rod 1 The ends of the annular holders 4 are arranged in a ring shape by a conventional injection molding process, or the ends of the ends of the swabs 1 can be respectively arranged as an annular support 4. Or, it may be arranged in an elliptical ring shape, a three-sided ring shape or a polygonal ring shape of at least three sides; the annular bracket 4 has a cylindrical cross section which is arranged in a circular shape, or may be arranged in an elliptical shape or a polygonal shape.
本实施例的环状支架 4 内环直径可设置在 1. 25〜； LO mm范围之 间， 本实施例设置为 3. 91 mm; 环状支架 4柱体横截面直径可设置在 0. 2〜3 mm范围之间， 本实施例设置为 1. 19 mm; 拭子杆 1和环状支 架 4可以采用聚乙烯塑料、聚对苯二甲酸类塑料或工程塑料， 本实施 例采用聚丙烯塑料材料制成，并根据需要在拭子杆 1部分设置有便于 定点折断拭子杆 1的折断口 3， 以方便采样后的传递使用； 或者也可 以采用改性的聚苯乙烯材料制造的拭子杆 1以实现在任意点折断。
以环状支架 4为基材，使植绒层 2通过常规静电植绒工艺覆盖在 环状支架 4基材的表面，环状支架 4基材表面的有效植绒层面积可设 置在 5〜296mm2范围之间， 本实施例为 46 mm2; 植绒层 2的纤维长 度可设置在 0.2〜3.0 mm范围之间， 本实施例为 0.6 mm; 植绒层 2 的纤维纤度可设置在 0.33〜7.0 dtex 范围之间， 本实施例为 1.5 dtex; 植绒层 2的纤维密度可设置在 1.5〜350 g/mm2范围之间， 本 实施例为 17 μ g/mm2; 拭子头植绒层 2涉及的材料为天然纤维， 或者 化学纤维， 或者二 的混合纤维， 本实施例为尼龙纤维。 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 Between the range of 〜3 mm, the embodiment is set to 1.19 mm; the swab rod 1 and the annular bracket 4 may be made of polyethylene plastic, polyterephthalic plastic or engineering plastic. In this embodiment, polypropylene plastic is used. Made of material, and if necessary, a baffle 3 for facilitating the breakage of the swab rod 1 is provided in the swab rod 1 to facilitate the transfer after sampling; or a swab made of modified polystyrene material can also be used. Rod 1 is achieved to break at any point. The annular scaffold 4 is used as a base material, and the flock layer 2 is covered on the surface of the base material of the annular support 4 by a conventional electrostatic flocking process, and the effective flocking layer area of the surface of the annular support 4 substrate can be set at 5 to 296 mm. Between the two ranges, this embodiment is 46 mm 2 ; the fiber length of the flock layer 2 can be set between 0.2 and 3.0 mm, which is 0.6 mm in this embodiment; the fiber fineness of the flock layer 2 can be set at 0.33~ Between 7.0 dtex range, this embodiment is 1.5 dtex; the fiber density of the flock layer 2 can be set between 1.5 and 350 g/mm 2 , which is 17 μ g/mm 2 in this embodiment; swab head flocking The material involved in layer 2 is a natural fiber, or a chemical fiber, or a mixed fiber of two, which is a nylon fiber in this embodiment.
与图 4所示传统拭子相比， 本实施例的优点表现为制造工艺简 单、 ί样量大、 能够确定定量吸附的液体体积为 55 μ ΐ ( 0@25°C)、 拭子头不易脱落、 使用方便和安全性好。 Compared with the conventional swab shown in Fig. 4, the advantage of this embodiment is that the manufacturing process is simple, the amount of the sample is large, and the volume of the liquid to be quantitatively adsorbed is determined to be 55 μ ΐ (0@25 ° C), and the swab head is not easy. Shedding, easy to use and safe.
实施例二， 环状支架 4内环直径 5 mm, 环状支架 4柱体横截面 直径 1.5 mm, 环状支架 4的基材有效植絨面积为 74 mm2， 尼龙纤维 长度 1.5 ran,纤维纤度 6.67 dtex,植绒层 2的纤维密度 328 μ g/mm2 , 吸附液体体积 110 μ ΐ (H20@25°C)， 其余同实施例一。 In the second embodiment, the annular support 4 has an inner ring diameter of 5 mm, the annular support 4 has a cylindrical cross-sectional diameter of 1.5 mm, the annular support 4 has an effective flocking area of 74 mm 2 , and the nylon fiber has a length of 1.5 ran, and the fiber has a fineness. 6.67 dtex, the fiber density of the flock layer 2 is 328 μg/mm 2 , and the volume of the adsorbed liquid is 110 μ ΐ (H 2 0@25 ° C), and the rest is the same as in the first embodiment.
实施例三， 环状支架 4内环直径 1.5 mm, 环状支架 4柱体横截 面直径 0.5 mm, 环状支架 4的基材有效植绒面积为 7.39 mm2， 尼龙 纤维长度 1.0 mm, 纤维纤度 1.5 dtex, 植绒层 2 的纤维密度 109 μ g/mm2, 吸附液体体积 20 μ ΐ (願 25°C)， 其余同实施例一。
Embodiment 3, the inner ring of the annular bracket 4 has a diameter of 1.5 mm, the annular bracket 4 has a cross-sectional diameter of 0.5 mm, the effective flocking area of the substrate of the annular bracket 4 is 7.39 mm 2 , the length of the nylon fiber is 1.0 mm, and the fiber fineness 1.5 dtex, the fiber density of the flock layer 2 is 109 μg/mm 2 , and the volume of the adsorbed liquid is 20 μ ΐ (25 ° C), and the rest is the same as in the first embodiment.
Claims
1、 定量生物采样植绒拭子， 包括拭子杆（1 )和拭子头， 其特征 在于： 拭子杆 （1 ) 的一端或两端的端部设置为环状支架 （4) 结构， 植绒 (2 ) 覆盖在环状支架 （4) 的表面形成拭子头。 1. A quantitative biological sampling flocking swab, comprising a swab rod (1) and a swab head, characterized in that: one end or both ends of the swab rod (1) are arranged as a ring bracket (4) structure, planted The velvet (2) covers the surface of the annular stent (4) to form a swab head.
2、根据权利要求 1所述的定量生物采样植绒拭子， 其特征在于: 环状支架 （4) 内环直径为 1. 25〜10 mm， 环状支架 （4) 柱体横截面 直径为 0. 2〜3皿； 或者环状支架 （4) 作为植绒基材的有效植绒面 积设置为 2. 5〜296 醒 2。 2. The quantitative biological sampling flocking swab according to claim 1, wherein: the annular support (4) has an inner ring diameter of 1. 25 to 10 mm, and the annular support (4) has a cross-sectional diameter of the cylinder. 0〜2小时醒2。 The effective flocking area of the flocking substrate is set to 2. 5~296 wake up 2 .
3、根据权利要求 2所述的定量生物采样植绒拭子， 其特征在于: 环状支架 （4) 设置成圆环形、 椭圆环形、 三边环形或至少三边以上 的多边环形， 环状支架 （4) 柱体横截面设置成圆形、 椭圆形或多边 形。 3. The quantitative biological sampling flocking swab according to claim 2, wherein: the annular support (4) is provided in a circular ring shape, an elliptical ring shape, a three-sided ring shape or a polygonal ring shape of at least three sides or more. Bracket (4) The cylinder cross section is set to be circular, elliptical or polygonal.
拭子杆 （1 ) 的材料为聚丙烯塑料、 聚乙烯塑料、 聚对苯二甲酸 类塑料、 聚苯乙烯塑料或工程塑料。 The material of the swab rod (1) is polypropylene plastic, polyethylene plastic, polybutylene plastic, polystyrene plastic or engineering plastic.
植绒层 （2 ) 采用天然纤维， 或者化学纤维， 或者二者的混合纤 维覆盖在环状支架 （4) 的表面。 The flock layer (2) is covered with natural fibers, or chemical fibers, or a mixture of the two on the surface of the annular stent (4).
4, .根据权利要求 1或 2或 3所述的定量生物采样植绒拭子， 其 特征在于： 植絨层 （2) 的纤维长度为 0. 2〜3. 0 mm， 植绒层 （2) 的 纤维纤度为 0. 33〜7. 0 dtex, 植绒层 （2 ) 的纤维密度为 1. 5〜350 μ g/醒 2。 2〜3. 0毫米,植植层(2), the fiber length of the flock layer (2) is 0. 2~3. 0 mm, flock layer (2 5〜350 μ g/醒了2。 The fiber density of the fiber density of 0. 33~7. 0 dtex, the fiber density of the flock layer (2) is 1. 5~350 μ g / wake up 2 .
5、根据权利要求 4所述的定量生物采样植绒拭子， 其特征在于： 植绒层 （2) 采用尼龙纤维覆盖在环状支架 （4) 的表面。 5. A quantitative biological sampling flocking swab according to claim 4, wherein: The flock layer (2) is covered with nylon fibers on the surface of the ring bracket (4).
6、根据权利要求 3所述的定量生物采样植绒拭子， 其特征在于: 拭子杆（1)上设置有便于定点折断拭子杆（1) 的折断口 （3)， 或用 改性的聚苯乙烯塑料制作的能任意点折断的拭子杆 （1) 上不设折断 口 （3 The quantitative biological sampling flocking swab according to claim 3, characterized in that: the swab rod (1) is provided with a fracture opening (3) for facilitating the fixed-point breaking of the swab rod (1), or modified The swab bar (1) made of polystyrene plastic can be broken at any point (1) without a break (3
7、 制造权利要求 1所述的定量生物采样植绒拭子的方法， 包括 拭子杆 （1) 和拭子头的制作， 其特征在于： 包括以下步骤， 通过注 塑工艺将拭子杆（1)制作成一端或两端呈环状支架（4)结构； 再以 环状支架（4)作为植绒基材， 在环状支架（4)基材的表面用常规静 电植绒工艺覆盖植绒层 （2)后制成拭子头， 植绒层 （2) 的材料采用 天然纤维， 或者化学纤维， 或者二者的混合纤维制作； 通过计算改变 环状 ί架 （4) 的有效植绒面积， 将环状支架 （4) 内环直径设计为 1.25〜10 mm, 将环状支架 （4) 柱体横截面直径设计成 0.2〜3 mm, 来实现控制有效植绒面积的值在 2.5〜296 mm2范围之间，最终得到采 样量能在 10〜200 μ 1范围之间选择的定量生物采样植绒拭子。 7. A method of producing a quantitative biological sampling flocking swab according to claim 1, comprising the preparation of a swab rod (1) and a swab head, characterized by: comprising the steps of: swabbing the swab by an injection molding process (1) Manufactured as one end or both ends in the form of a ring-shaped bracket (4); the ring-shaped bracket (4) is used as a flocking substrate, and the surface of the ring-shaped bracket (4) is covered with a conventional electrostatic flocking process. The layer (2) is made into a swab head, and the material of the flock layer (2) is made of natural fiber, or chemical fiber, or a mixed fiber of the two; the effective flocking area of the ring-shaped frame (4) is changed by calculation The inner ring diameter of the annular bracket (4) is designed to be 1.25~10 mm, and the cross-sectional diameter of the annular bracket (4) is designed to be 0.2~3 mm, so as to realize the effective flocking area of 2.5 to 296. Between the range of mm 2 , a quantitative bioscattered flocked swab with a sample size between 10 and 200 μ 1 was finally obtained.
8、 根据权利要求 7或 8所述的制造定量生物采样植绒拭子的方 法， 其特征在于： 将植绒层 （2) 纤维长度控制为 0.2〜3.0 ram, 将 植绒层 （2) 纤维纤度控制为 0.33〜7.0 dtex, 将植绒层 （2) 植绒 纤维密度控制为 1.5〜350 μ g/ram2。 The method for manufacturing a quantitative biological sampling flocking swab according to claim 7 or 8, wherein: the fiber length of the flock layer (2) is controlled to be 0.2 to 3.0 ram, and the flock layer (2) fiber is used. The fineness is controlled to be 0.33 to 7.0 dtex, and the density of the flocked layer (2) flocking fiber is controlled to 1.5 to 350 μg/ram 2 .
9、 根据权利要求 7所述的制造定量生物采样植绒拭子的方法， 其特征在于： 综合控制拭子头的环状支架 （4) 结构和植绒密度实现 拭子头定量采集液体样本， 在 25°C条件下重量分析法测得的拭子头 吸附去离子水的体积范围为在 10〜200 μ 1之间的确定值。 9. The method for manufacturing a quantitative biological sampling flocking swab according to claim 7, wherein: the swab head is quantitatively collected to comprehensively control the structure and the flocking density of the swab head, and the liquid sample is quantitatively collected by the swab head. Swab head measured by gravimetric analysis at 25 ° C The volume of adsorbed deionized water ranges from a certain value between 10 and 200 μl.
10、 根据权利要求 9所述的制造定量生物采样植绒拭子的方法， 其特征在于： 植绒层 （2) 的材料采用尼龙纤维。 10. A method of manufacturing a quantitative biologically sampled flocked swab according to claim 9, wherein: the material of the flock layer (2) is nylon fiber.
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| US13/989,056 US20130245495A1 (en) | 2010-12-24 | 2011-02-09 | Flocking swab for biological sampling quantitatively and method for making the same |
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| CN201010604583.0A CN102087172B (en) | 2010-12-24 | 2010-12-24 | Quantitative biological sampling flocking swab |
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| CN201010604572.2A CN102138810B (en) | 2010-12-24 | 2010-12-24 | The method manufacturing quantitative biological sampling flocking swab |
| CN201020679116.X | 2010-12-24 | ||
| CN201020679116XU CN201993241U (en) | 2010-12-24 | 2010-12-24 | Quantitative biological sampling flocked swab |
| CN201010604583.0 | 2010-12-24 |
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