WO2012077024A1

WO2012077024A1 - Composition for inhibiting the growth of mammalian hair

Info

Publication number: WO2012077024A1
Application number: PCT/IB2011/055431
Authority: WO
Inventors: Mrinmayee Kondhalkar
Original assignee: Mrinmayee Kondhalkar
Priority date: 2010-12-08
Filing date: 2011-12-02
Publication date: 2012-06-14
Also published as: GB201311617D0; GB2499769A; AU2011340160A1; GB2499769B; AU2011340160B2; GB2499769A8

Abstract

The invention relates to inhibiting hair growth. Particularly, the invention relates to composition for and method of inhibiting growth of mammalian hair with plant extract using liposomal delivery system which damages the hair follicle, thereby disabling it to form a normal hair shaft.

Description

Compositions for inhibiting mammalian hair growth

FIELD OF INVENTION:

The invention relates to inhibiting hair growth.

Particularly, the invention relates to composition for and method of inhibiting growth of mammalian hair with plant extract using liposomal delivery system which damages the hair follicle , thereby disabling it to form a normal hair shaft.

INTRODUCTION:

Generally normal body hair growth is tolerated. But if there is excessive unwanted hair growth it affects the self-esteem of the person because of the social stigma.

Various procedures have been employed to remove unwanted hair, including shaving, electrolysis, depilatory creams or lotions, waxing, plucking, and therapeutic anti-androgens. These conventional procedures generally have drawbacks associated with them.

PRIOR ART:

Asa rule normal body hair growth is tolerated. But if there is excessive unwanted hair growth it affect st he self-esteem of the person. Various procedures have been employed to remove unwanted hair, including shaving, depilatory creams or lotion, waxing, plucking, electrolysis, therapeutic ant i-androgens and laser. These convent ional procedures general ly have drawbacks associated with t hem.

The i nventor herself has fi l ed fol lowing patents on t he subj ect , i ncorporated here as reference.

Indian patent application no IN/ 837/ 2004 dated 13^th August 2004

(Granted Patent no: 237834) USpatent publicat ion no US 2006-0034952 A1 dated 16^th February 2006 USpatent publicat ion no US 2008-0145330 A1 dated 19^th June 2008 PCT patent application no PCT/ IN2005/ 000190 10^th June 2005

South Af rica Granted patent no: 2007/ 2024

United Kingdom Granted Patent no: GB2434533

Australia Patent Application no: 2005270843 (Granted) Indian Patent Applicat ion No 337/ MUIW 2006 dated 09^th March 2006 Indian Patent Applicat ion No 998/ MUIW 2006 dated 16^th March 2006 Indian Patent Applicat ion No 1296/ MUM/ 2006 dated 21^st August 2006 The inventor has discussed the use of the actives in different forms derived from the castor beans and related group of actives (cytotoxic lectins) for the inhibition of hair and hair follicle related actions in these patent applications.

Castor bean is well known for its lipid constituent, castor oil and its medicinal properties especially as a purgative.

The ancient science of Ayurveda describes many medicines made of castor oil and beans for various applications such as for general pain, inflammation, headache, abdominal pain, fever, asthma, cough, leucoderma, piles, Vaatnashak, used externally as a paste to treat inflammatory skin conditions, boils, carbuncles, abscesses, inflammation of the middle ear, and migraines.

Though the topical applications of castor are well known, they are not meant for the above-mentioned application.

Most of all, the above prior art other than patent applications filed by Inventor, does not mention use of the composition for specifically inhibit mammalian hair growth. Whereas, the claimed composition for specifically inhibiting mammalian hair growth is a novel invention and would offer an inexpensive method for inhibiting mammalian hair growth.

This invention seeksto overcome the limitations of the prior art. The object of this invention is to provide for a composition for inhibiting mammalian hair growth.

Another object of this invention is to provide for a composition for inhibiting mammalian hair growth from castor beans.

Another object of this invention is to provide for a composition for inhibiting mammalian hair growth from follicular delivery of aqueous extract of castor beans.

Another object of this invention is to provide for a composition using vesicular carriers which deliver the active i.e. Rein in the extract directly to the hair follicle without causing allergic reaction.

Another object of this invention is to provide for a cytotoxic lectin (e.g. Rein) composition using vesicular carriers for hair growth inhibition wherein purification of cytotoxic lectin (e.g. Rein) is not necessary.

Another object of this invention is to provide for a composition for inhibiting mammalian hair growth from follicular delivery of liposomized castor beans extract. Another object of this invention is to provide for a composition that could be applied topically to the skin so as to damage the follicle, resulting in follicular destruction, thereby disabling it to form a hair shaft.

Another object of this invention is to provide the composition in the form of a paste, cream or gel which is easy to apply, has aesthetic appeal and has good spread ability.

Another object of this invention is to provide for a composition in the form of a paste, cream or gel that leaves no stains on application and isfree of preservatives.

Another object of this invention is to provide for a composition which could be packed conveniently in jars or tubes.

E3ACKGROUND OF THE INVENTION

The invention relates to inhibiting hair growth.

Particularly, the invention relates to composition for and method of inhibiting growth of mammalian hair with plant extract using liposomal delivery system which damages the hair follicle, thereby disabling it to form a normal hair shaft.

SUMMARY OF THE INVENTION The present invention comprises of compositions for topical application for inhibiting unwanted mammalian hair growth by applying an effective amount of the composition to the skin of individuals in need to inhibit hair, including facial areas, legs, arms, armpits, and torso; irrespective of gender of the subject. The composition comprises of a hair-growth- inhibiting active agent dispersed in a dermatologically acceptable vehicle such as but not limiting to oil in water emulsion, gels, sustained release polymer films, sprays, soaps etc. Similarly, vesicular carriers like liposomes can be used for the selective follicular delivery of the hair-growth-inhibiting agent.

TECHNICAL FIELD

Rein, which represents the type II ribosome inactivating proteins, is the active ingredient in the castor bean that inactivates the hair follicle disabling it to form the hair shaft. Apart from ricin & its isomers, there is a group of allergens that are present in the castor bean. Rein is water-soluble in nature, but also is present in castor oil in low concentrations. So, both aqueous extract and oil fraction can be used together or separately for the hair-growth inhibition. However, the allergic fraction causes both immediate and delayed hypersensitivity reactions. So, it calls for the treatment of the extract to remove the allergens. Various extraction and purification procedures of the herbal extract are well known to those skilled in the art. However, the present invention describes an innovative solution wherein castor bean extract, without purifying Rein, can be used by encapsulating in a carrier like liposome, neosome or microspheres. Use of such vesicular carriers results in delivery of the active i.e. Rein in the extract directly to the hair follicle without causing allergic reaction.

Before administration of the composition, herein described, in a preferred embodiment the hair is removed from the follicle e.g. by waxing or plucking or alternatively by other methods like shaving, depilation. Methods of hair removal which remove the hair from the follicle are known to initiate anagen, the growth phase of the hair cycle and also it exposes the hair follicle assembly responsible for the synthesis of hair shaft to the action of the composition herein described.

The topical compositions are the formulations containing therapeutically effective amounts of the respective active agent and pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions such as but not limiting to pH adjusting and buffering agents, toxicity adjusting agents and the like, vesicular carriers like liposomes, microspheres, neosomes for carrying the active agent to the hair follicle, along with the usual components of the particular carrier like e.g. for oil in water emulsion, stearic acid and its derivatives, cetyl alcohol and its derivatives, mineral oils, citric acid, preservatives, fragrance, colours etc. or other such topical compositions as described in different pharmaceutical publications, resulting in aesthetically pleasing and easy to use formulations.

-Examples:

Crude aqueous extracts of castor beans are used for liposome formulations. Compositions:

NNuummbbeerr ooff ggeell aanndd ooiill iinn wwaatteerr eemmui.lsions were prepared using varying concentrations of the lliippoossoommee ssuussppeennssiioonn ffoorr aanniimmaall aass wwell as human experiments.

Oil in water emulsion: Mineral oil 26% Cety I alcohol 0.52% 9 ear ic acid 0.94% Get ost eary I al cohol 0.94 %

&>dium alginate 0.36% Triethanol amine 0.1 %

Methyl Paraben 0.2% Propyl paraben 0.01 %

Purified Water q.s.

Liposome suspension 10- 50 % Gel:

Carbopol 940 1 %

Triethanol amine 1 %

Purified Water q.s. Liposome suspension 10- 50 %

Liposome encapsulation Examplel:

Liposomes were prepared using Phospholipon 90 G (Lipoid) 10 mg and cholesterol 1.5 mg by ethanol injection method.

Example 2:

Liposomes were prepared using Phospholipon 90 G (Lipoid) 10 mg and cholesterol 1.5 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by cent rifugat ion.

Example 3:

Liposomes were prepared using Phospholipon 90 G (Lipoid) 15 mg by thin film formation method and hydrated with the extract (15 ml). Un-encapsulated extract is removed by cent rifugat ion.

Example 4

Liposomes were prepared using Phospholipon 90 G (Lipoid) 10 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by cent rifugat ion.

Example 5

Liposomes were prepared using &ya lecithin (Himedia) 10 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by cent rifugat ion. Example 6

Liposomes were prepared using Soya lecithin (Himedia) 9 mg and DOPE 1 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by centrifugation.

Example 7

Liposomes were prepared using Soya lecithin (Himedia) 8 mg and DOPE 2 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by centrifugation.

All the formulations mentioned above showed reduction in the number of hair follicles, reduction in the darkness, length and thickness of the hair. Overall they changed the appearance of re-growing hair, both in animal experiments (mice) and humans. Also, the subjects did not show any adverse reaction to their skin.

At the end of the description of the invention, it should be understood that without departing from the spirit of the invention or the essential characteristics, it might be embodied in other variations. The embodiments described here should be considered as illustrative but not as restrictive.

All variations and forms, which come within the meaning and the range of equivalency of the claim, are intended to be embraced therein.

Claims

1. Atopical composition for inhibiting the growth of mammalian hair comprising

a. Hair growth inhibiting active agent which is a liposome-encapsulated Ricinus communis extract

b. Dermatologically acceptable vehicle

c. Water (q.s.),

wherein the hair growth inhibiting active agent isdispersed in a dermatologically acceptable vehicle.

2. A topical composition for inhibiting mammalian hair growth as claimed in claim 1 wherein the composition is in the form of oil in water emulsion or gel.

3. According to claim 1, wherein the liposomes are prepared using phospholipids

4. According to claim 3, wherein the phospholipids used are selected from lecithin, phosphatidylcholine (PC), phosphatidylethanolamine(PE), phosphatidylserine(PS), DOPE, ceramide.

5. According to claim 1, wherein the liposomes optionally contain cholesterol or cholesteryl esters.

6. A cosmetic method of inhibiting mammalian hair growth, comprising providing and applying a topical composition of any of the preceding claim to mammalian skin.

7. A cosmetic method of inhibiting mammalian hair growth as claimed in claim 6, wherein the topical composition is applied after removal of hair.

8. A cosmetic method of inhibiting mammalian hair growth as claimed in claim 6, wherein the skin is human skin.

9. Atopical composition and cosmetic method for inhibiting mammalian hair growth as claimed in any of the preceding claims and substantially described with reference to the accompanying examples.