AU2011340160A1

AU2011340160A1 - Composition for inhibiting the growth of mammalian hair

Info

Publication number: AU2011340160A1
Application number: AU2011340160A
Authority: AU
Inventors: Mrinmayee Kondhalkar
Original assignee: Individual
Current assignee: Individual
Priority date: 2010-12-08
Filing date: 2011-12-02
Publication date: 2013-07-18
Anticipated expiration: 2031-12-02
Also published as: WO2012077024A1; GB2499769A; GB201311617D0; GB2499769B; GB2499769A8; AU2011340160B2

Abstract

The invention relates to inhibiting hair growth. Particularly, the invention relates to composition for and method of inhibiting growth of mammalian hair with plant extract using liposomal delivery system which damages the hair follicle, thereby disabling it to form a normal hair shaft.

Description

WO 2012/077024 PCT/IB2011/055431 Compositions for inhibiting mammalian hair growth FIELD OF INVENTION: The invention relates to inhibiting hair growth. Particularly, the invention relates to composition for and method of inhibiting growth of mammalian hair with plant extract using liposomal delivery system which damages the hair follicle , thereby disabling it to form a normal hair shaft. INTRODUCTION: Generally normal body hair growth is tolerated. But if there is excessive unwanted hair growth it affects the self-esteem of the person because of the social stigma. Various procedures have been employed to remove unwanted hair, including shaving, electrolysis, depilatory creams or lotions, waxing, plucking, and therapeutic anti-androgens. These conventional procedures generally have drawbacks associated with them. PRIOR ART: As a rule normal body hair growth is tolerated. But if there is excessive unwanted hair growth it af f ect s t he self -est eem of t he person. 1 WO 2012/077024 PCT/IB2011/055431 Various procedures have been employed to remove unwanted hair, including shaving, depilatory creams or lotion, waxing, plucking, electrolysis, therapeutic anti-androgens and laser. These conventional procedures generally have drawbacks associated with them. The invent or herself has fil ed f following pat ent s on t he subj ect, incorporat ed here as ref erence. Indian patent application no IN/ 837/ 2004 dated 13 1h August 2004 (Granted Patent no: 237834) USpatent publication no US2006-0034952 Al dated 16 1h February 2006 USpatent publication no US2008-0145330 Al dated 19 1h June 2008 PCT patent application no PCT/ IN2005/ 000190 10 1h June 2005 Ebuth Africa Granted patent no: 2007/ 2024 United Kngdom Granted Patent no: GB2434533 Australia Patent Application no: 2005270843 (Granted) Indian Pat ent Applicat ion No 337/ MUNW 2006 dat ed 09 h March 2006 Indian Pat ent Applicat ion No 998/ MUNW 2006 dat ed 16 h March 2006 Indian Pat ent Applicat ion No 1296/ MUNW 2006 dat ed 21' August 2006 2 WO 2012/077024 PCT/IB2011/055431 The inventor has discussed the use of the actives in different forms derived from the castor beans and related group of actives (cytotoxic lectins) for the inhibition of hair and hair follicle related actions in these patent applications. Castor bean is well known for its lipid constituent, castor oil and its medicinal properties especially as a purgat ive. The ancient science of Ayurveda describes many medicines made of castor oil and beans for various applications such as for general pain, inflammation, headache, abdominal pain, fever, asthma, cough, leucoderma, piles, Vaatnashak, used externally as a paste to treat inflammatory skin conditions, boils, carbuncles, abscesses, inflammation of the middle ear, and migraines. Though the topical applications of castor are well known, they are not meant for the above-mentioned application. Most of all, the above prior art other than patent applications filed by Inventor, does not mention use of the composition for specifically inhibit mammalian hair growth. Whereas, the claimed composition for specifically inhibiting mammalian hair growth is a novel invention and would offer an inexpensive method for inhibiting mammalian hair growth. This invention seeksto overcome the limitations of the prior art. 3 WO 2012/077024 PCT/IB2011/055431 The object of this invention is to provide for a composition for inhibiting mammalian hair growth. Another object of this invention isto provide for a composition for inhibiting mammalian hair growth from castor beans. Another object of this invention isto provide for a composition for inhibiting mammalian hair growth from follicular delivery of aqueous extract of castor beans. Another object of this invention isto provide for a composition using vesicular carriers which deliver the active i.e. Rcin in the extract directly to the hair follicle without causing allergic react ion. Another object of this invention is to provide for a cytotoxic lectin (e.g. Rcin) composition using vesicular carriers for hair growth inhibition wherein purification of cytotoxic lectin (e.g. Rcin) is not necessary. Another object of this invention isto provide for a composition for inhibiting mammalian hair growth from follicular delivery of liposomized castor beans extract. 4 WO 2012/077024 PCT/IB2011/055431 Another object of this invention is to provide for a composition that could be applied topically to the skin so as to damage the follicle, resulting in follicular destruction, thereby disabling it to form a hair shaft. Another object of this invention is to provide the composition in the form of a paste, cream or gel which is easy to apply, has aesthetic appeal and has good spread ability. Another object of this invention is to provide for a composition in the form of a paste, cream or gel that leaves no stains on application and isfree of preservatives. Another object of this invention is to provide for a composition which could be packed conveniently in jars or tubes. BACKGROUND OF THE INVENTION The invention relates to inhibiting hair growth. Particularly, the invention relates to composition for and method of inhibiting growth of mammalian hair with plant extract using liposomal delivery system which damages the hair follicle, thereby disabling it to form a normal hair shaft. SUMMARY OF THE INVENTION 5 WO 2012/077024 PCT/IB2011/055431 The present invention comprises of compositions for topical application for inhibiting unwanted mammalian hair growth by applying an effective amount of the composition to the skin of individuals in need to inhibit hair, including facial areas, legs, arms, armpits, and torso; irrespective of gender of the subject. The composition comprises of a hair-growth inhibiting active agent dispersed in a dermatologically acceptable vehicle such as but not limiting to oil in water emulsion, gels, sustained release polymer films, sprays, soaps etc. Smilarly, vesicular carriers like liposomes can be used for the selective follicular delivery of the hair-growth-inhibiting agent. TECHNICAL FIELD Rcin, which represents the type || ribosome inactivating proteins, is the active ingredient in the castor bean that inactivates the hair follicle disabling it to form the hair shaft. Apart from ricin & its isomers, there is a group of allergens that are present in the castor bean. Rcin is water-soluble in nature, but also is present in castor oil in low concentrations. D, both aqueous extract and oil fraction can be used together or separately for the hair-growth inhibition. However, the allergic fraction causes both immediate and delayed hypersensitivity reactions. %, it calls for the treatment of the extract to remove the allergens. Various extraction and purification procedures of the herbal extract are well known to those skilled in the art. However, the present invention describes an innovative solution wherein castor bean extract, without purifying Rcin, can be used by encapsulating in a carrier like liposome, 6 WO 2012/077024 PCT/IB2011/055431 neosome or microspheres. Use of such vesicular carriers results in delivery of the active i.e. Fcin in the extract directly to the hair follicle without causing allergic reaction. Before administration of the composition, herein described, in a preferred embodiment the hair is removed from the follicle e.g. by waxing or plucking or alternatively by other methods like shaving, depilation. Methods of hair removal which remove the hair from the follicle are known to initiate anagen, the growth phase of the hair cycle and also it exposes the hair follicle assembly responsible for the synthesis of hair shaft to the action of the composition herein described. The topical compositions are the formulations containing therapeutically effective amounts of the respective active agent and pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions such as but not limiting to pH adj usting and buffering agents, toxicity adjusting agents and the like, vesicular carriers like liposomes, microspheres, neosomes for carrying the active agent to the hair follicle, along with the usual components of the particular carrier like e.g. for oil in water emulsion, stearic acid and its derivatives, cetyl alcohol and its derivatives, mineral oils, citric acid, preservatives, fragrance, colours etc. or other such topical compositions as described in different pharmaceutical publications, resulting in aesthetically pleasing and easy to use formulations. Examples: Crude aqueous extracts of castor beans are used for liposome formulations. 7 WO 2012/077024 PCT/IB2011/055431 Compositions: Number of gel and oil in water emulsions were prepared using varying concentrations of the liposome suspension for animal as well as human experiment s. 01 in water emulsion: Mineral oil 26% Cetyl alcohol 0.52% Searic acid 0.94% Cetostearyl alcohol 0.94 % &>dium alginate 0.36% Triethanolamine 0.1 % Methyl Paraben 0.2% Propyl paraben 0.01 % Purified Water q. s. Liposome suspension 10- 50 % Gel: Carbopol 940 1 % Triethanolamine 1 % Purified Water q.s. 8 WO 2012/077024 PCT/IB2011/055431 Liposome suspension 10- 50 % Liposome encapsulat ion Example: Liposomes were prepared using Phospholipon 90 G (Lipoid) 10 mg and cholesterol 1.5 mg by ethanol injection method. Example 2: Liposomes were prepared using Phospholipon 90 G (Lipoid) 10 mg and cholesterol 1.5 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by cent rif ugat ion. Example 3: Liposomes were prepared using Phospholipon 90 G (Lipoid) 15 mg by thin film formation method and hydrated with the extract (15 ml). Un-encapsulated extract is removed by cent rif ugat ion. Example 4 Liposomes were prepared using Phospholipon 90 G (Lipoid) 10 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by cent rif ugat ion. Example 5 Liposomes were prepared using Ebya lecithin (Himedia) 10 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by centrifugation. 9 WO 2012/077024 PCT/IB2011/055431 Example 6 Liposomes were prepared using Soya lecithin (Himedia) 9 mg and DOPE 1 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by centrifugat ion. Example 7 Liposomes were prepared using Soya lecithin (Himedia) 8 mg and DOPE 2 mg by thin film formation method and hydrated with the extract (10 ml). Un-encapsulated extract is removed by centrifugat ion. All the formulations mentioned above showed reduction in the number of hair follicles, reduction in the darkness, length and thickness of the hair. Overall they changed the appearance of re-growing hair, both in animal experiments (mice) and humans. Aso, the subjects did not show any adverse reaction to their skin. At the end of the description of the invention, it should be understood that without departing from the spirit of the invention or the essential characteristics, it might be embodied in other variations. The embodiments described here should be considered as illustrative but not as restrictive. Al variations and forms, which come within the meaning and the range of equivalency of the claim, are intended to be embraced therein. 10

Claims

1. Atopical composition for inhibiting the growth of mammalian hair comprising a. Hair growth inhibiting active agent which is a liposome-encapsulated Ricinus communis ext r act b. Dermatologically acceptable vehicle c. Water (q.s.), wherein the hair growth inhibiting active agent isdispersed in adermatologically acceptable vehicle.

2. A topical composition for inhibiting mammalian hair growth as claimed in claim 1 wherein the composition is in the form of oil in water emulsion or gel.

3. According to claim 1, wherein the liposomes are prepared using phospholipids

4. According to claim 3, wherein the phospholipids used are selected from lecithin, phosphat idylcholine (PC), phosphat idylet hanol ami ne(PE), phosphat idylseri ne(PS), DOPE, ceramide.

5. According to claim 1, wherein the liposomes optionally contain cholesterol or cholesteryl esters.

6. A cosmetic method of inhibiting mammalian hair growth, comprising providing and applying a topical composition of any of the preceding claim to mammalian skin.

7. A cosmetic method of inhibiting mammalian hair growth as claimed in claim 6, wherein the topical composition is applied after removal of hair.

8. A cosmetic method of inhibiting mammalian hair growth as claimed in claim 6, wherein the skin is human skin.

9. A topical composition and cosmetic method for inhibiting mammalian hair growth as claimed in any of the preceding claims and substantially described with reference to the accompanying examples. 11