WO2012049154A8 - Qualitätsbestimmung von stammzellen - Google Patents

Qualitätsbestimmung von stammzellen Download PDF

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Publication number
WO2012049154A8
WO2012049154A8 PCT/EP2011/067697 EP2011067697W WO2012049154A8 WO 2012049154 A8 WO2012049154 A8 WO 2012049154A8 EP 2011067697 W EP2011067697 W EP 2011067697W WO 2012049154 A8 WO2012049154 A8 WO 2012049154A8
Authority
WO
WIPO (PCT)
Prior art keywords
genes
stem cells
cpg
quality determination
pluripotent stem
Prior art date
Application number
PCT/EP2011/067697
Other languages
English (en)
French (fr)
Other versions
WO2012049154A1 (de
Inventor
Simeon Santourlidis
Original Assignee
Wernet, Peter
Groth, Nicole
Ghanjati, Foued
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wernet, Peter, Groth, Nicole, Ghanjati, Foued filed Critical Wernet, Peter
Priority to JP2013533179A priority Critical patent/JP2014500708A/ja
Priority to EP11767256.8A priority patent/EP2627779A1/de
Priority to US13/878,915 priority patent/US20140004512A1/en
Publication of WO2012049154A1 publication Critical patent/WO2012049154A1/de
Publication of WO2012049154A8 publication Critical patent/WO2012049154A8/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Transplantation (AREA)
  • Developmental Biology & Embryology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Verfahren zur Qualitätsbestimmung einer pluripotenten Stammzelle umfassend die Schritte: Messen der DNA-Methylierung mindestens eines CpG in einer CpG-Insel in mindestens zwei Genen der pluripotenten Stammzelle, Vergleich mit der DNA-Methylierung des mindestens einen CpG in einer CpG-Insel in den mindestens zwei Genen mindestens einer Referenzzelle wobei die Gene auf verschiedenen Chromosomen lokalisiert sind und zur Genfamilie der olfaktorischen Rezeptorgene gehören.
PCT/EP2011/067697 2010-10-11 2011-10-11 Qualitätsbestimmung von stammzellen WO2012049154A1 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2013533179A JP2014500708A (ja) 2010-10-11 2011-10-11 幹細胞の品質判定
EP11767256.8A EP2627779A1 (de) 2010-10-11 2011-10-11 Qualitätsbestimmung von stammzellen
US13/878,915 US20140004512A1 (en) 2010-10-11 2011-10-11 Quality determination of stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10187137.4 2010-10-11
EP10187137 2010-10-11

Publications (2)

Publication Number Publication Date
WO2012049154A1 WO2012049154A1 (de) 2012-04-19
WO2012049154A8 true WO2012049154A8 (de) 2013-04-18

Family

ID=43530173

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/067697 WO2012049154A1 (de) 2010-10-11 2011-10-11 Qualitätsbestimmung von stammzellen

Country Status (4)

Country Link
US (1) US20140004512A1 (de)
EP (1) EP2627779A1 (de)
JP (1) JP2014500708A (de)
WO (1) WO2012049154A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016060264A1 (ja) * 2014-10-17 2016-04-21 積水メディカル株式会社 幹細胞の品質管理方法
JP6886893B2 (ja) * 2017-08-23 2021-06-16 花王株式会社 嗅覚受容体の応答測定方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2482907A1 (en) * 2002-04-16 2003-10-30 Origene Technologies, Inc. Tissue specific genes and gene clusters
WO2007026255A2 (en) * 2005-06-22 2007-03-08 Universitetet I Oslo Dedifferentiated cells and methods of making and using dedifferentiated cells
WO2010069008A1 (en) * 2008-12-19 2010-06-24 Griffith University A germline competent cell derived from adult tissue
US20120128655A1 (en) * 2009-04-03 2012-05-24 The Mclean Hospital Corporation Induced pluripotent stem cells

Also Published As

Publication number Publication date
JP2014500708A (ja) 2014-01-16
US20140004512A1 (en) 2014-01-02
WO2012049154A1 (de) 2012-04-19
EP2627779A1 (de) 2013-08-21

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