WO2012043711A1 - Agent favorisant la production du collagène de la peau - Google Patents

Agent favorisant la production du collagène de la peau Download PDF

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Publication number
WO2012043711A1
WO2012043711A1 PCT/JP2011/072359 JP2011072359W WO2012043711A1 WO 2012043711 A1 WO2012043711 A1 WO 2012043711A1 JP 2011072359 W JP2011072359 W JP 2011072359W WO 2012043711 A1 WO2012043711 A1 WO 2012043711A1
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WIPO (PCT)
Prior art keywords
amino acid
milk protein
protein fraction
milk
skin collagen
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PCT/JP2011/072359
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English (en)
Japanese (ja)
Inventor
加藤　健
愛子 小野
宏 上野
典子 上田
松山　博昭
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雪印メグミルク株式会社
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Publication of WO2012043711A1 publication Critical patent/WO2012043711A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/19—Dairy proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
    • A61K8/986—Milk; Derivatives thereof, e.g. butter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/007—Preparations for dry skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/83—Electrophoresis; Electrodes; Electrolytic phenomena

Definitions

  • the present invention relates to a skin collagen production promoter, a food and drink for promoting skin collagen production, and a cosmetic for promoting skin collagen production, which are useful for preventing rough skin, wrinkles, a decrease in elasticity, and the like. More specifically, the present invention relates to a skin collagen production promoter containing a milk protein fraction and / or a degradation product of the milk protein fraction as an active ingredient.
  • substances having a skin collagen promoting action include a basic protein fraction present in milk or a basic peptide fraction obtained by degrading the basic protein fraction with a proteolytic enzyme (Patent Document 1), lactoferrin Alternatively, a lactoferrin degradation product obtained by degrading lactoferrin with a proteolytic enzyme (Patent Document 2), lactoperoxidase or a lactoperoxidase degradation product obtained by degrading lactoperoxidase with a proteolytic enzyme (Patent Document 3), angio An angiogenin degradation product obtained by degrading genin or angiogenin with a proteolytic enzyme (Patent Document 4) is already known.
  • the present invention provides a new skin collagen production promoter that promotes the biosynthesis of skin collagen that can be used as a food material that can prevent dryness, rough skin, wrinkles, and talmi, and that is safe in terms of safety.
  • the issue is to provide.
  • this invention makes it a subject to provide the food / beverage products or cosmetics which mix
  • the inventors of the present invention have been diligently searching for substances that have a skin collagen production promoting action widely contained in food materials. A fraction and / or a degradation product of the milk protein fraction was found and the present invention was completed.
  • a skin collagen production promoter comprising a milk protein fraction having the following characteristics (a) to (c) as an active ingredient.
  • A It is derived from milk.
  • B A fraction containing a protein having a molecular weight in the range of 80,000 to 85,000 daltons by electrophoresis on sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE).
  • C The basic amino acid is contained in the constituent amino acid composition in an amount of 16 to 18% by weight, and the basic amino acid / acidic amino acid ratio is in the range of 0.65 to 0.85.
  • a skin collagen production promoter comprising a degradation product of a milk protein fraction having the following characteristics (a) to (c) as an active ingredient.
  • A It is derived from milk.
  • B A fraction containing a protein having a molecular weight in the range of 80,000 to 85,000 daltons by electrophoresis on sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE).
  • C The basic amino acid is contained in the constituent amino acid composition in an amount of 16 to 18% by weight, and the basic amino acid / acidic amino acid ratio is in the range of 0.65 to 0.85.
  • a skin collagen production promoting cosmetic comprising the milk protein fraction according to (1) or (2) or a degradation product of the milk protein fraction.
  • the milk protein fraction having the following characteristics (a) to (c) is orally ingested at a dose of 10 ⁇ g or more per day per adult, or becomes 0.001 to 2% by weight based on the total amount of the composition
  • B A fraction containing a protein having a molecular weight in the range of 80,000 to 85,000 daltons by electrophoresis on sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE).
  • the basic amino acid is contained in the constituent amino acid composition in an amount of 16 to 18% by weight, and the basic amino acid / acidic amino acid ratio is in the range of 0.65 to 0.85.
  • the degradation product of the milk protein fraction having the following characteristics (a) to (c) is orally ingested in an amount of 10 ⁇ g or more per day per adult, or 0.001-2 weight based on the total amount of the composition A method for promoting the production of skin collagen by applying an agent formulated so as to be at a percentage.
  • A It is derived from milk.
  • (B) A fraction containing a protein having a molecular weight in the range of 80,000 to 85,000 daltons by electrophoresis on sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE).
  • (C) The basic amino acid is contained in the constituent amino acid composition in an amount of 16 to 18% by weight, and the basic amino acid / acidic amino acid ratio is in the range of 0.65 to 0.85.
  • the skin collagen production promoter of the present invention promotes the biosynthesis of collagen in the skin by oral administration or application, it is useful for preventing dry skin, rough skin, wrinkles, lowering elasticity, and the like.
  • the skin collagen production promoter of the present invention is (a) derived from milk, and (b) sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) electrophoresis, having a molecular weight in the range of 80,000 to 85,000 daltons.
  • a fraction containing protein (c) 16 to 18% by weight of a basic amino acid in the constituent amino acid composition and a basic amino acid / acidic amino acid ratio in the range of 0.65 to 0.85
  • the active ingredient is a milk protein fraction that satisfies all three conditions, or a degradation product of the milk protein fraction.
  • Such a milk protein fraction is prepared by, for example, bringing milk material such as skim milk or whey into contact with a cation exchange resin, washing it with a 0.6 M salt solution, and then washing the milk protein adsorbed on the resin with 1. It can be obtained by eluting with a salt eluent of 0 to 1.5M.
  • a salt eluent 0 to 1.5M.
  • potassium salt, ammonia salt, phosphate, acetate, carbonate, etc. can be used in addition to sodium chloride, the ionic strength of the washing solution is 0.55 to 0.65, and the ionic strength of the elution solution Can be appropriately adjusted to 1.0 or more, preferably 1.0 to 2.0, to obtain the milk protein fraction of the present invention.
  • this elution fraction can be collected, desalted and concentrated by reverse osmosis (RO) membrane, electrodialysis (ED) method or the like, and dried if necessary.
  • RO reverse osmosis
  • ED electrodialysis
  • Examples of the reverse osmosis (RO) membrane include Desal-3 (manufactured by Desalination), HR-95 (manufactured by Dow Danmark), NTR-729HF (manufactured by Nitto Denko), and the like.
  • electrodialysis (ED) device include electrodialysis devices manufactured by Yuasa Ionics and Nippon Ruisui.
  • a method for obtaining a protein fraction derived from milk after bringing milk or a milk-derived raw material into contact with a cation exchanger, the basic protein fraction adsorbed on the cation exchanger exceeds pH 5, A method of elution with an eluent with an ionic strength of more than 0.5 (Japanese Patent Laid-Open No. 5-202098), a method of using an alginate gel (Japanese Patent Laid-Open No. 61-246198), milk using inorganic porous particles A method for obtaining from milk (Japanese Patent Laid-Open No.
  • the milk protein fraction having a skin collagen production promoting action used in the present invention contains 16 to 18% by weight of basic amino acids in its constituent amino acid composition, and the basic amino acid / acidic amino acid ratio is 0.65 to 0.005. A range of 75. Outside this range, the effects of the present invention cannot be exhibited. Further, the product of the present invention is a protein-based protein mixture having a molecular weight of 80,000 to 85,000 daltons and an isoelectric point of 7.0 to 8.0.
  • the degradation product of the milk protein fraction has the same amino acid composition as that of the milk protein fraction.
  • a protease such as pepsin, trypsin or chymotrypsin acts on the milk protein fraction obtained by the above method.
  • a degradation product having an average molecular weight of 4,000 or less can be obtained by acting a proteolytic enzyme such as pancreatin.
  • the degradation product of the milk protein fraction can also be used after being pulverized by freeze drying or the like.
  • milk such as cow's milk, human milk, goat milk, and sheep milk can be used. These milks can be used as they are, or reduced milk, skim milk, whey and the like of these milks can be used.
  • the skin collagen production promoter of the present invention exhibits an effect of promoting skin collagen production by oral administration or application.
  • the active ingredient milk protein fraction or the degradation product of the milk protein fraction can be used as it is. It can also be formulated into a medicine, tablet, capsule, drink or the like.
  • oral preparations such as powders, granules, tablets and capsules are formulated by conventional methods using excipients such as starch, lactose, sucrose, mannitol, carboxymethylcellulose, corn starch, and inorganic salts. It becomes.
  • binders in addition to the above-mentioned excipients, binders, disintegrants, surfactants, lubricants, fluidity promoters, colorants, fragrances and the like can be appropriately used.
  • the binder include starch, dextrin, gum arabic powder, gelatin, hydroxypropyl starch, sodium carboxymethylcellulose, methylcellulose, crystalline cellulose, ethylcellulose, and polyvinylpyrrolidone.
  • disintegrant include starch, hydroxypropyl starch, carboxymethylcellulose, sodium carboxymethylcellulose, crosslinked sodium carboxymethylcellulose, and crystalline cellulose.
  • soybean lecithin As surfactant, soybean lecithin, sucrose fatty acid ester, etc., as lubricant, talc, wax, sucrose fatty acid ester, hydrogenated vegetable oil, etc., as fluidity promoter, anhydrous silicic acid, dry aluminum hydroxide,
  • examples include magnesium silicate.
  • these milk protein fractions or degradation products of the milk protein fractions can be blended in nutrients, foods and drinks, etc. as they are or after preparation. Moreover, if a milk protein fraction or a degradation product of a milk protein fraction is blended together with components that are conventionally considered to have an effective action for collagen production such as vitamin C, further skin collagen production promoting action can be expected. . In addition, since the milk protein fraction or the degradation product of the milk protein fraction is relatively stable to heat, it can be sterilized by heating under the conditions usually used.
  • the skin collagen production promoter of the present invention When applying the skin collagen production promoter of the present invention, it is prepared into various dosage forms such as a liquid agent, a solid agent, a semi-solid agent, etc., by blending with commonly used known components according to the purpose of use.
  • Preferred compositions include ointments, gels, creams, sprays, patches, lotions, powders and the like.
  • the skin collagen production promoter of the present invention is a hydrocarbon such as petrolatum, a higher fatty acid lower alkyl ester such as stearyl alcohol, isopropyl myristate, an animal fat such as lanolin, a polyhydric alcohol such as glycerin, a glycerin fatty acid ester, a monoester
  • a hydrocarbon such as petrolatum, a higher fatty acid lower alkyl ester such as stearyl alcohol, isopropyl myristate, an animal fat such as lanolin, a polyhydric alcohol such as glycerin, a glycerin fatty acid ester, a monoester
  • surfactants such as stearic acid and polyethylene glycol
  • inorganic salts such as stearic acid and polyethylene glycol
  • waxes such as stearic acid and polyethylene glycol
  • preservatives such as methyl paraoxybenzoate and butyl paraoxybenzoate And can
  • the effective amount of the skin collagen production promoter of the present invention by oral administration is appropriately defined by the formulation form, administration method, purpose of use, and age, weight, and medical condition of the patient to which it is applied, but is not constant. According to the results of animal experiments, it was found that it was necessary to ingest 10 ⁇ g or more of the milk protein fraction or the degradation product of the milk protein fraction per kg body weight of the rat in order to show the skin collagen production promoting action. Therefore, according to the extrapolation method, the effect can be expected by ingesting a milk protein fraction or a degradation product of milk protein fraction of 10 ⁇ g or more per adult per day. What is necessary is just to mix
  • the effective amount of the application of the skin collagen production promoter of the present invention varies depending on the dosage form, but is preferably from 0.001 to 2% by weight based on the total amount of the composition to be applied. What is necessary is just to mix
  • the eluate was desalted and concentrated by reverse osmosis (RO) membrane treatment, and then freeze-dried to obtain a powdered milk protein fraction. This operation was repeated twice to obtain 104 g of protein fraction.
  • the isoelectric point of this protein fraction was 7.0 to 8.5, and the basic amino acid contained in this protein fraction was 17.8%.
  • the eluate was desalted and concentrated by reverse osmosis (RO) membrane treatment, and then freeze-dried to obtain a powdered milk protein fraction. This operation was repeated twice to obtain 48 g of milk protein fraction.
  • This fraction contains a protein having a molecular weight of 80,000 to 85,000 daltons, and has an isoelectric point of 7.0 to 8.0.
  • basic amino acids are 16-18%.
  • the basic amino acid / acidic amino acid ratio was 0.65 to 0.85.
  • the obtained milk protein fraction can be used as it is as the skin collagen production promoter of the present invention.
  • the eluate was desalted and concentrated by reverse osmosis (RO) membrane treatment, and then freeze-dried to obtain a powdered milk protein fraction.
  • This operation was performed once to obtain 22.1 g of milk protein fraction.
  • This fraction contains a protein having a molecular weight of 80,000 to 85,000 daltons and has an isoelectric point of 7.0 to 8.0.
  • basic amino acids was 16-18%.
  • the basic amino acid / acidic amino acid ratio was 0.65 to 0.85.
  • the obtained milk protein fraction can be used as it is as the skin collagen production promoter of the present invention.
  • the eluate was desalted and concentrated by reverse osmosis (RO) membrane treatment, and then freeze-dried to obtain a powdered milk protein fraction.
  • This operation was performed twice to obtain 48.8 g of milk protein fraction.
  • This fraction contains a protein having a molecular weight of 80,000 to 85,000 daltons and has an isoelectric point of 7.0 to 8.0.
  • basic amino acids was 16-18%.
  • the basic amino acid / acidic amino acid ratio was 0.65 to 0.85.
  • the obtained milk protein fraction can be used as it is as the skin collagen production promoter of the present invention.
  • [Test Example 1] Collagen production of the milk protein fraction obtained in Reference Example 1, the milk protein fraction obtained in Example 1, and the degradation product of the milk protein fraction obtained in Example 4 by animal experiments using rats The promoting effect was examined. Seven-week-old Wistar male rats were obtained in the physiological saline administration group (Group A), the group (Group B) in which the milk protein fraction obtained in Reference Example 1 was administered at 10 ⁇ g / kg of the rat body weight, and Reference Example 1. The group (group C) in which 100 ⁇ g of the obtained milk protein fraction was administered per kg of rat body weight, the group (group D) in which the milk protein fraction obtained in Example 1 was administered at 10 ⁇ g per kg of rat body weight were obtained in Example 1.
  • Group (group E) in which 100 ⁇ g of the obtained milk protein fraction was administered per kg body weight of the rat (group E), Group (group F) in which the degradation product of the milk protein fraction obtained in Example 4 was administered at 10 ⁇ g per kg of body weight of the rat, Examples The degradation product of the milk protein fraction obtained in 4 was divided into 7 test groups (n 6) in a group (group G) administered with 100 ⁇ g per kg body weight of rats, and each was administered once daily with a sonde and reared for 10 weeks. It was. Regarding the amount of collagen in the skin, the rat dermis was treated according to the method of Nimni et al. (See Arch. Biochem.
  • hydroxyproline is a special amino acid contained only in collagen and accounts for about 10% of all amino acids constituting collagen, so that the amount of collagen can be estimated (see Takashi Asano et al., Bio Industry, p. 12, 2001). The results are shown in Table 1.
  • * indicates that there is a significant difference compared with the control group A (p ⁇ 0.05).
  • the amount of hydroxyproline in the soluble fraction after 10 weeks was significantly higher in the B group, C group, D group, E group, F group and G group than in the A group.
  • the milk protein fraction obtained in Example 1 and the degradation product of the milk protein fraction obtained in Example 4 are about twice as effective as the milk protein fraction obtained in Reference Example 1. I found out. This revealed that the milk protein fraction of the present invention or a degradation product of the milk protein fraction has a skin collagen production promoting action, and was shown to be useful as a skin collagen production promoter. It was also revealed that this skin collagen production promoting action was observed when a milk protein fraction or a degradation product of the milk protein fraction was administered at least 10 ⁇ g per kg body weight of the rat.
  • Beverages containing the skin collagen production promoter with the formulation shown in Table 2 were produced by conventional methods.
  • the flavor of the manufactured beverage was good, and the flavor did not deteriorate even after storage at room temperature for 1 year, and there was no problem such as precipitation.
  • a dough having the composition shown in Table 3 was prepared by a conventional method, molded, and then baked to produce a biscuit containing a skin collagen production promoter.
  • Each component was mixed with the composition shown in Table 5 and emulsified at an emulsification temperature of 85 ° C. to prepare a processed cheese containing a skin collagen production promoter.
  • Example 2 About the milk protein fraction obtained in Example 2 and Example 3 (Example product 2 and Example product 3) and the degradation product of the milk protein fraction obtained in Example 5 (Example product 5), normal Skin collagen production promoting action was examined by an experiment using a human fibroblast cell line [CCD45SK (ATCCRL 1506) collected from the skin of a white woman].
  • a human fibroblast cell line [CCD45SK (ATCCRL 1506) collected from the skin of a white woman].
  • FBS fetal bovine serum
  • Example Product 2 and Example Product 3 the milk protein fraction obtained in Example 2 and Example 3
  • Example Product 5 the degradation product of the milk protein fraction obtained in Example 5
  • ⁇ -aminopropionitrile is 50 ⁇ g / ml
  • tritium-L-proline is 1 ⁇ Ci / ml.
  • the culture solution was further cultured for 24 hours to obtain a culture solution.
  • the collagen fraction was fractionated from the culture medium thus obtained according to the method of Webster et al. (See Analytical Biochemistry, page 220, 1979), and the radioactivity incorporated into the collagen fraction was measured. As a control, the same test was performed without adding the milk protein fraction and the degradation product of the milk protein fraction. The results are shown in Table 6.
  • * indicates that there is a significant difference compared to the control group (p ⁇ 0.05).
  • the milk protein fraction and the group to which the degradation product of the milk protein fraction was added were twice as much as the group to which the milk protein fraction and the degradation product of the milk protein fraction were not added (control).
  • the above collagen production promoting ability was demonstrated. This reveals that the milk protein fraction and the degradation product of the milk protein fraction of the present invention have an action of acting on dermal fibroblasts and promoting collagen production, and are useful as a skin collagen production promoter. It was shown that there is.
  • a lotion having the composition shown in Table 7 was produced by a conventional method.
  • a cream having the composition shown in Table 8 was produced by a conventional method.
  • Example 3 Using the lotion obtained in Example 10 and the cream obtained in Example 11, an actual use test was conducted.
  • a comparative product the same product as in Examples 10 and 11 was used except that the milk protein fraction and the degradation product of the milk protein fraction were excluded.
  • the skin lotion of the product of the present invention is significantly improved in dry feeling, rough skin, etc., and excellent in promoting skin collagen production, compared to the skin lotion of the comparative product.
  • the cream of the present invention also has an improvement in dry feeling and a marked improvement in rough skin, as compared with the comparative cream, and has been found to have an effect of suppressing natural deterioration such as rough skin.

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Abstract

La présente invention concerne un nouvel agent favorisant la production du collagène de la peau destiné à favoriser la biosynthèse du collagène de la peau, qui est capable de prévenir l'apparition d'une peau sèche, d'une peau rugueuse, de rides et d'un relâchement de la peau, et qui peut également être utilisé comme substance alimentaire sans aucun problème de sécurité. La présente invention concerne également le problème consistant à fournir des aliments, des boissons et des substances cosmétiques contenant l'agent favorisant la production du collagène de la peau. L'agent favorisant la production du collagène de la peau utilise des fractions de protéines du lait ayant les caractéristiques (a) à (c) suivantes comme principe actif. (a) Les fractions de protéines du lait dérivent du lait. (b) Les fractions, obtenues par le biais d'une électrophorèse sur gel de polyacrylamide en présence de sulfate de sodium dodécylique (SDS-PAGE), contiennent des protéines ayant des poids moléculaires dans la plage allant de 80 000 à 85 000 daltons. (c) La composition constituante à base d'acides aminés contient de 16 à 18 % en poids d'acides aminés basiques, et le rapport acides aminés basiques/acides aminés acides se situe dans la plage allant de 0,65 à 0,85.
PCT/JP2011/072359 2010-09-30 2011-09-29 Agent favorisant la production du collagène de la peau WO2012043711A1 (fr)

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JP2010222096A JP2012077015A (ja) 2010-09-30 2010-09-30 皮膚コラーゲン産生促進剤

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003144095A (ja) * 2001-11-08 2003-05-20 Snow Brand Milk Prod Co Ltd 美肌剤
WO2004098632A1 (fr) * 2003-05-07 2004-11-18 Snow Brand Milk Products Co., Ltd. Promoteur de la production du collagene de la peau
JP2007246413A (ja) * 2006-03-14 2007-09-27 Snow Brand Milk Prod Co Ltd 乳由来塩基性タンパク質含有組成物
WO2009057287A1 (fr) * 2007-11-01 2009-05-07 Snow Brand Milk Products Co., Ltd. Matière d'aliment pour inhiber la création d'ostéoclaste

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003144095A (ja) * 2001-11-08 2003-05-20 Snow Brand Milk Prod Co Ltd 美肌剤
WO2004098632A1 (fr) * 2003-05-07 2004-11-18 Snow Brand Milk Products Co., Ltd. Promoteur de la production du collagene de la peau
JP2007246413A (ja) * 2006-03-14 2007-09-27 Snow Brand Milk Prod Co Ltd 乳由来塩基性タンパク質含有組成物
WO2009057287A1 (fr) * 2007-11-01 2009-05-07 Snow Brand Milk Products Co., Ltd. Matière d'aliment pour inhiber la création d'ostéoclaste

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