WO2012031562A1 - Virtual navigation assisted biopsy model for tumor tissue unable to be displayed by ultrasonography - Google Patents

Abstract

A virtual navigation assisted biopsy model for tumor tissue unable to be displayed by ultrasonography is provided. The model consists of tumor gelatin and substantial gelatin. The substantial gelatin is made of carrageenan. The tumor gelatin is made by adding iodized oil, surfactant and milk into carrageenan. The model is formed by allowing the substantial gelatin to envelop one or more cut tumor gelatin blocks with the volume of 0.5-2 cm3. The model can, in virtual navigation technology, realistically simulate tumor tissue which can not be displayed by ultrasonography, and surrounding normal tissue, and effectively lead the virtual navigation technology to in real time assist the positioning and biopsy of tumor tissue which cannot be displayed by ultrasonography. The model enables simple operation and accurate results, and is adapted for learning and training for virtual navigation technology.

Description

 A model for tumor biopsy process that cannot be displayed by virtual navigation-assisted ultrasound

 Technical field

 The invention belongs to the field of medical and health, and relates to Ultrasound-guided interventional therapy in the field of ultrasound, specifically related to a model of tumor tissue biopsy process that cannot be displayed by virtual navigation-assisted ultrasound.

 Background technique

Ultrasound-guided biopsy of liver tumor tissue is a common clinical technique. In this technique, ultrasound mainly plays a role in localizing tumors. The premise of using ultrasound guidance is that ultrasound can show tumors, but it is affected by factors such as disorder of liver tissue, echoes of lesions, etc. In actual operation, ultrasound often fails to show tumor lesions. CT and ultrasound have different imaging principles. When ultrasound cannot show tumors, CT can often be displayed. However, the current CT is a static imaging and is not suitable for guiding biopsy in real time.

 Virtual navigation technology can use magnetic localization methods to fuse image information of different types or different times, such as three-dimensional ultrasound volume images and CT of the same patient. The three-dimensional volume images were fused or fused with a three-dimensional ultrasound volume image of the same patient at different times. After the fusion, the three-dimensional data displayed by the two images can completely coincide in space, and the two images that are overlapped can be simultaneously displayed on the same screen, and the clinically useful information can be found by comparing the two images.

 Virtual navigation technology successfully combines real-time imaging of ultrasound with high-resolution imaging of CT, and extracts the advantages of both through image fusion technology. CT images assisted with ultrasound for real-time localization to assist biopsy of tumors that ultrasound cannot display, and is a highly clinically valuable technique.

The principle of virtual navigation technology is completely different from other imaging methods in the past, including more physics concepts, such as spatial magnetic localization and image fusion. Such concepts are more abstract and less easily understood by imaging doctors, affecting the mastery of this useful technique.

 In vitro models can play an important role in the promotion of a new technology. Some foreign scholars use the commercial colloid to make virtual navigation guidance. Ultrasound can not display tumors. Biopsy phantoms, but such ultrasonic phantoms are expensive and cannot be reused. Another foreign scholar uses a model of isolated bovine liver, but this model requires CT. The evaluation of puncture accuracy, the operation is relatively complicated, and the elasticity of the isolated tissue is large, and it is easy to deform during the operation, which affects the accuracy of the puncture, so it is not suitable for the learning and training of virtual navigation technology. At present, there is no research report on related content in China.

 Summary of the invention

 The object of the present invention is to provide a tumor biopsy process for virtual navigation-assisted ultrasound that cannot be displayed in view of the deficiencies of the prior art. The model is used to promote the learning and training of virtual navigation technology.

 Another object of the present invention is to provide a method of preparing the model.

 The above object of the present invention is achieved by the following technical solutions:

 The invention provides A model for a biopsy process of a tumor tissue that cannot be displayed by virtual navigation-assisted ultrasound, characterized in that the model consists of one or more tumor gels wrapped by a parenchymal gel The parenchymal gel is made of carrageenan, and the tumor gel is made of carrageenan, lipiodol, surfactant and milk.

 The above two kinds of glues are respectively obtained by the following raw materials and methods:

 The parenchy gum is a carrageenan obtained by mixing carrageenan dry product with hot physiological saline at a mass ratio of 1:45-55. The physiological saline solution is stirred and cooled to prepare.

 Tumor gel is made up of carrageenan - physiological saline solution with lipiodol, surfactant and milk according to the mass ratio (150-400) :1 : ( Mix 3-5): (15-40) in proportion (become carrageenan - saline - lipiodol - surfactant - milk solution), made by cooling.

 In this model, the tumor gel is cut into small pieces of volume 0.5-2 cm3, and the cut tumor mass is wrapped with parenchymal glue.

 The marking material is also attached to the substantial glue, and is a toothpick-shaped article made of bamboo, wood or plastic. The preferred marking material is a toothpick.

 The invention also provides a preparation method of the model, comprising the following steps:

 (1) Preparation of carrageenan - physiological saline solution, carrageenan - saline - lipiodol - surfactant - Milk solution.

 (2) Carrageenan - normal saline - lipiodol - surfactant - milk solution is cooled and solidified and cut into volume 0.5-2 Cubic centimeter of tumor gel block; the tumor gel block was placed in a carrageenan-physiological saline solution and cooled to prepare a model. In Carrageenan - After the physiological saline solution is cooled and molded, a marker article is inserted therein as a registration mark.

 The colloid used in the present invention has the following advantages in the application of the virtual navigation assisted tissue biopsy process:

 ( 1 The invention selects carrageenan as the main raw material of the model. Carrageenan is a polysaccharide extracted from seaweed. Its food grade dry colloid is cheap and is a commonly used material in the food and pharmaceutical industries. Carrageenan produced in Hainan, China belongs to Κ- family, this family of carrageenan has good thermal reversibility, the melting point of solid carrageenan and the freezing point of liquid carrageenan at 40-60 ° C The carrageenan can be reshaped between, ie, simple heating or cooling. The colloid has the advantages of being easy to obtain, low in cost, low in production conditions, good in sound permeability, and difficult to deform the solid rubber. Proper proportion of carrageenan - The physiological saline solution has a suitable hardness, elasticity and brittleness after being turned into a solid, and is not easily deformed after being molded into a mold, and can be made into a substantive gel.

 (2) The present invention uses lipiodol as an additive to tumor gel. Iodine oil can significantly increase the density of tissue, and it can improve CT after adding colloid. Value. The surfactant is combined with lipiodol to distribute the lipiodol evenly in the liquid carrageenan. When the gel becomes solid, the ultrasonic echo is unevenly added. CT The value has increased significantly. The tumor gel was embedded in the parenchyma. Because the ultrasonic echoes of the two gels were the same, the ultrasound could not show the tumor gel. However, the CT value of the tumor gel was significantly higher than that of the parenchyma, so CT A scan reveals the tumor mass in the parenchymal gel.

 (3 Adding a proper amount of milk to the carrageenan solution added with lipiodol, the gel becomes milky white, and the tumor gel and the parenchyma can be distinguished by the naked eye; the ultrasonic echo of the colloid does not change significantly after adding the milk.

 Carrageenan - The physiological saline solution is added with lipiodol, surfactant and milk to make tumor gel. After the tumor gel is embedded in the ordinary parenchyma, the gel can not be displayed by ultrasonic scanning, and CT It can be clearly displayed during the scan. The naked eye is generally colorless and translucent, while the tumor gel is white and opaque, which can be clearly distinguished by the naked eye.

 Thus, the model of the present invention can accurately simulate the inability of the ultrasound to be displayed in the virtual navigation technique. Tumor tissue, effective and objective real-time guided tissue biopsy: tumor gel, ultrasound can not be displayed, CT scan can be used to simulate tumor lesions; tumor gel wrapped around the periphery of the gel, ultrasound and CT Can be displayed and can be identified with the naked eye and tumor glue, can simulate the normal tissue surrounding the tumor; the tumor gel in the model (simulated tumor lesion) can be used for tissue biopsy The detected rubber strip can be observed with the naked eye, and the difference from the actual glue is obvious to verify the effect of the virtual navigation assisted biopsy. At the same time, the invention further explores the material used as a virtual navigation alignment mark in the solid rubber block model. For toothpick-like articles made of bamboo, wood or plastic, it is found that the ultrasonic image can be clearly displayed, and the present invention selects an ordinary toothpick. After the solid glue is embedded in the tumor gel and solidified into a solid, a toothpick is inserted into the parison. The toothpick can be clearly displayed on the ultrasound image and can be used for the alignment mark.

 Compared with the prior art, the present invention has the following beneficial effects:

(1) The model provided by the present invention can accurately simulate that ultrasound cannot be displayed but CT The tumor tissue that can be displayed effectively and objectively simulates virtual navigation technology to assist in locating the tumor tissue that cannot be displayed by biopsy ultrasound, and realizes effective learning and training of virtual navigation technology.

(2) The biopsy results obtained by the model of the present invention are accurate and the biopsy operation process is simple.

(3) The model provided by the invention has low production cost, and the raw materials used are low-cost raw materials and have wide sources.

(4) The preparation method of the model of the invention is simple, does not require large or advanced equipment, and is highly implementable.

 DRAWINGS

 figure 1 For the biopsy needle, the top of the strip is the actual strip (shown by the left arrow), the left side of the middle strip is the tumor strip, and the right half is the strip (the right arrow), most Below is the tumor strip (shown by the up arrow), and the different strips can be distinguished by the naked eye.

 Figure 2 shows the ultrasound image of the model after the fusion of the ultrasound image and the CT image. The tumor gel is not seen in the model. The right side of the model is the CT of the model. Figure, see the tumor gel in the model (shown by the up arrow).

 detailed description

 The technical solution of the present invention will be further described below by way of specific examples.

 Example

 The dried carrageenan produced in Hainan was dissolved in hot physiological saline at a ratio of 1:50, and all dissolved into carrageenan-physiological saline solution for use.

 Iodine oil, detergent, milk and carrageenan - physiological saline solution by 1 : 5 : 20 : 200 The ratio is mixed and cooled to solidify into a tumor gel. The solidified tumor gel is cut into a volume of 1 cubic centimeter of rubber and placed in carrageenan - In the physiological saline solution, after the solution is solidified, the bamboo toothpick is dispersed vertically into the parison gel to form a model.

 One side of the toothpick marking block is a CT scan of the head end. Import Dicom format CT images into Mylab 90 In the ultrasound system, the bamboo toothpick is high-density in the CT image and high-echo in the ultrasound image. As the positioning marker, the ultrasound is combined with the CT image, and the tumor is used for biopsy (Fig. 2). Shown).

After the biopsy is completed, the results of the virtual navigation assisted biopsy are verified by observing the biopsy strips. Different results can be distinguished by the naked eye: a completely transparent parison strip representing normal tissue; and a semi-substantial half-substantial half-substantial half parenchyma representing a semi-lesion Semi-tumor strip; a fully milky white tumor strip representing a complete lesion (as shown 1 shown).

 From the visual observation after biopsy, it is clear that virtual navigation technology assists in the localization of tumors that cannot be displayed by ultrasound. Biopsy the results of tumor tissue to achieve the purpose of learning and training virtual navigation technology.

Claims (8)

1. A model for a biopsy process of a tumor tissue that cannot be displayed by virtual navigation-assisted ultrasound, characterized in that the model consists of one or more tumor glues wrapped by a parenchymal gel; the parenchymal gel is made of carrageenan; the tumor gel is made of carrageenan It is made by mixing lipiodol, surfactant and milk.
2. The model according to claim 1, wherein said substantive glue is a mass ratio of dry carrageenan to thermophysiological saline 1: 45-55 The ratio of the obtained carrageenan - physiological saline solution was stirred and cooled.
3. The model according to claim 2, wherein said tumor gel is a mass ratio of carrageenan-physiological saline solution to lipiodol, surfactant, and milk. The ratio of 150-400:1:3-5:15-40 is mixed and cooled.
4. The model of claim 1 wherein said tumor gel is in a volume of 0.5-2 A small piece of cubic centimeter, wrapped with one or more tumor blocks with parenchymal glue.
5. The model of claim 1 wherein said substantially adhesive is provided with a marking material.
6. The model of claim 5 wherein said The marking material is a toothpick-like article made of bamboo, wood or plastic.
7. A method for preparing a model for a virtual navigation assisted tissue biopsy process, comprising the steps of:

   (1) Preparation of carrageenan - physiological saline solution, carrageenan - saline - lipiodol - surfactant - Milk solution

   (2) Carrageenan - normal saline - lipiodol - surfactant - milk solution is cooled and solidified and cut into a volume of 0.5-2 Cubic centimeter of tumor gel block; the tumor gel block is placed in a mold, poured into a carrageenan-physiological saline solution, and then cooled to form a model.
8. The preparation method according to claim 8, wherein the carrageenan-physiological saline solution of the step (2) is cooled and molded, and then inserted therein. A toothpick-like item made of bamboo, wood or plastic.

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