WO2012030827A1 - Élément régulateur pour la production de protéines hétérologues dans l'organe fructifère de champignons filamenteux - Google Patents

Élément régulateur pour la production de protéines hétérologues dans l'organe fructifère de champignons filamenteux Download PDF

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Publication number
WO2012030827A1
WO2012030827A1 PCT/US2011/049741 US2011049741W WO2012030827A1 WO 2012030827 A1 WO2012030827 A1 WO 2012030827A1 US 2011049741 W US2011049741 W US 2011049741W WO 2012030827 A1 WO2012030827 A1 WO 2012030827A1
Authority
WO
WIPO (PCT)
Prior art keywords
expression
promoter
nucleotide sequence
gene
sequence
Prior art date
Application number
PCT/US2011/049741
Other languages
English (en)
Inventor
Zhixin Shi
Jack Q. Wilkinson
Donald S. Walters
C. Peter Romaine
Original Assignee
Agarigen, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agarigen, Inc. filed Critical Agarigen, Inc.
Priority to CA2809828A priority Critical patent/CA2809828C/fr
Priority to CN201180051472.4A priority patent/CN103180433B/zh
Priority to US13/819,356 priority patent/US9145561B2/en
Publication of WO2012030827A1 publication Critical patent/WO2012030827A1/fr
Priority to US14/851,819 priority patent/US10131916B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Abstract

L'invention concerne des compositions et des méthodes pouvant réguler l'expression de séquences nucléotidiques chez des champignons. Ces compositions sont de nouvelles séquences nucléotidiques pour un promoteur à préférence tissulaire, isolé du gène de la lectine d'Agaricus bisporus. Ces séquences s'expriment, de préférence, dans le tissu de l'organe fructifère. L'invention concerne également une méthode utilisant ces séquences régulatrices pour exprimer une séquence nucléotidique chez des champignons. La méthode consiste à transformer une cellule fongique de sorte qu'elle comprenne une séquence nucléotidique liée de manière fonctionnelle à une ou plusieurs des séquences régulatrices de la présente invention; et à régénérer un champignon transformé de manière stable à partir de la cellule transformée.
PCT/US2011/049741 2010-08-30 2011-08-30 Élément régulateur pour la production de protéines hétérologues dans l'organe fructifère de champignons filamenteux WO2012030827A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2809828A CA2809828C (fr) 2010-08-30 2011-08-30 Element regulateur pour la production de proteines heterologues dans l'organe fructifere de champignons filamenteux
CN201180051472.4A CN103180433B (zh) 2010-08-30 2011-08-30 用于在丝状真菌子实体中产生异源蛋白的调控元件
US13/819,356 US9145561B2 (en) 2010-08-30 2011-08-30 Regulatory element for heterologous protein production in the fruiting body of filamentous fungi
US14/851,819 US10131916B2 (en) 2010-08-30 2015-09-11 Regulatory element for heterologous protein production in the fruiting body of filamentous fungi

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87092010A 2010-08-30 2010-08-30
US12/870,920 2010-08-30

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/819,356 A-371-Of-International US9145561B2 (en) 2010-08-30 2011-08-30 Regulatory element for heterologous protein production in the fruiting body of filamentous fungi
US14/851,819 Continuation US10131916B2 (en) 2010-08-30 2015-09-11 Regulatory element for heterologous protein production in the fruiting body of filamentous fungi

Publications (1)

Publication Number Publication Date
WO2012030827A1 true WO2012030827A1 (fr) 2012-03-08

Family

ID=45773239

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/049741 WO2012030827A1 (fr) 2010-08-30 2011-08-30 Élément régulateur pour la production de protéines hétérologues dans l'organe fructifère de champignons filamenteux

Country Status (3)

Country Link
CN (1) CN103180433B (fr)
CA (1) CA2809828C (fr)
WO (1) WO2012030827A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10131916B2 (en) 2010-08-30 2018-11-20 Intrexon Corporation Regulatory element for heterologous protein production in the fruiting body of filamentous fungi

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104611337B (zh) * 2015-02-21 2018-02-13 吉林农业大学 低温诱导型启动子pcp1及应用
CN105602953A (zh) * 2016-03-09 2016-05-25 浙江农林大学 双孢蘑菇诱导型启动子和表达载体及其应用
CN112225787B (zh) * 2017-06-09 2022-01-04 福建省平潭县水产良种实验有限公司 一种抑制棕囊藻生长的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060073560A1 (en) * 2002-10-31 2006-04-06 Kerry Burton Selective expression in filamentous fungi
US20070014817A1 (en) * 2005-05-03 2007-01-18 Institut Pasteur Methods for detecting virulent Plasmodium, for evaluating Plasmodium virulence, and for screening new drugs employing the 3'UTR of Plasmodium SUB2 and the Plasmodium SUB2 serine protease

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* Cited by examiner, † Cited by third party
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EP1173578A2 (fr) * 1999-05-04 2002-01-23 Monsanto Company Compositions polypeptidiques toxiques pour les coleopteres et plantes transgeniques resistant aux insectes
CN101440377A (zh) * 2008-11-11 2009-05-27 中国热带农业科学院热带生物技术研究所 一种在果实中特异表达白藜芦醇的植物表达载体

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060073560A1 (en) * 2002-10-31 2006-04-06 Kerry Burton Selective expression in filamentous fungi
US20070014817A1 (en) * 2005-05-03 2007-01-18 Institut Pasteur Methods for detecting virulent Plasmodium, for evaluating Plasmodium virulence, and for screening new drugs employing the 3'UTR of Plasmodium SUB2 and the Plasmodium SUB2 serine protease

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [online] 15 January 2009 (2009-01-15), "Mouse DNA sequence from clone RP23-48L13 on chromosome X, complete sequence.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/AL732435 Database accession no. AL732435 *
DATABASE GENBANK [online] 5 March 2010 (2010-03-05), "Clostridium perfringens ATCC 13124, complete genome.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/110673209?report=genbank Database accession no. CP000246 *
DATABASE GENBANK [online] 7 August 2001 (2001-08-07), "ENTOK56TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, genomic survey sequence", retrieved from http://www.ncbi.nlm.nih.gov/nucgss/BH138942 Database accession no. BH 138942 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10131916B2 (en) 2010-08-30 2018-11-20 Intrexon Corporation Regulatory element for heterologous protein production in the fruiting body of filamentous fungi

Also Published As

Publication number Publication date
CN103180433B (zh) 2015-05-13
CA2809828A1 (fr) 2012-03-08
CN103180433A (zh) 2013-06-26
CA2809828C (fr) 2019-01-29

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