WO2012019104A1 - Méthode et dispositif d'immunoessai impliquant la capture de billes sensibles au magnétisme - Google Patents

Méthode et dispositif d'immunoessai impliquant la capture de billes sensibles au magnétisme Download PDF

Info

Publication number
WO2012019104A1
WO2012019104A1 PCT/US2011/046753 US2011046753W WO2012019104A1 WO 2012019104 A1 WO2012019104 A1 WO 2012019104A1 US 2011046753 W US2011046753 W US 2011046753W WO 2012019104 A1 WO2012019104 A1 WO 2012019104A1
Authority
WO
WIPO (PCT)
Prior art keywords
beads
magnet
sample
electrode
conduit
Prior art date
Application number
PCT/US2011/046753
Other languages
English (en)
Inventor
Cary James Miller
Original Assignee
Abbott Point Of Care Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Point Of Care Inc. filed Critical Abbott Point Of Care Inc.
Priority to EP11746727.4A priority Critical patent/EP2600973B1/fr
Priority to CN201180046212.8A priority patent/CN103118785B/zh
Publication of WO2012019104A1 publication Critical patent/WO2012019104A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Definitions

  • the present invention generally relates to an apparatus and method for rapid determination of analytes in liquid samples by immunoassays incorporating magnetic capture of beads on a sensor, capable of being used in the point-of-care diagnostic field, including, for example, use at accident sites, emergency rooms, in surgery, in intensive care units, and also in non-medical environments.
  • Electrochemical detection in which the binding of an analyte directly or indirectly causes a change in the activity of an electroactive species adjacent to an electrode, has also been applied to immunoassays.
  • electrochemical immunoassays see Laurell et al., Methods in Enzymology, vol. 73, "Electroimmunoassay", Academic Press, New York, 339, 340, 346-348 ( 1981).
  • electroactive species may be attached directly to an analyte, or the antibody may be covalently attached to an enzyme that either produces an electroactive species from an electroinactive substrate or destroys an electroactive substrate. See, M. J. Green ( 1987) Philos. Trans. R. Soc. Lond. B. Biol. Sci. 316: 135-142, for a review of electrochemical immunosensors. Magnetic components have been integrated with electrochemical immunoassays.
  • Microfabrication techniques are attractive for constaiction of multilayered sensor staictures in confined spaces.
  • Methods for microfabrication of electrochemical immunosensors, for example on silicon substrates are disclosed in U.S. Pat. No. 5,200,051 to Cozette et al, which is hereby incorporated in its entirety by reference. These include dispensing methods, methods for attaching biological reagent, e.g., antibodies, to surfaces including photoformed layers and microparticle latexes, and methods for performing electrochemical assays.
  • U.S. Pat. No. 7,223,438 to Mirkin et al. describes a method of forming magnetic nanostaictures by depositing a precursor onto a substrate using a nanoscopic tip, and then converting the precursor to form a magnetic nanostructure.
  • U.S. Pat. No. 7, 106,051 to Prins et al. describes a magnetoresistive sensing device for determining the density of magnetic particles in a fluid.
  • U.S. Pat. Appl. Pub. 2009/0191401 to Deetz et al. is directed to magnetic receptive paints and coatings that allow magnets to stick to coated surfaces. These paint and coating compositions contain multiple-sized ferromagnetic particles and a base resin with minimal or no fillers and provide an ultra smooth finish on a substrate.
  • U.S. Pat. No. 5,587, 102 to Stern et al. discloses a latex paint composition comprising iron particles and U.S. Pat. No. 5,843,329 to Deetz provides techniques for blending magnetic receptive particles into solution for making magnetic coatings.
  • Jointly-owned U.S. Pat. Nos. 5,998,224 and 6,294,342 to Rohr et al. disclose assay methods utilizing the response of a magnetically responsive reagent to influence a magnetic field to qualitatively or quantitatively measure binding between specific binding pair members. Each of these patents is hereby incorporated by reference in its entirety.
  • the present invention is directed to a magnetic immunosensing device and methods of performing an immunoassay with imniunosensors incorporating magnetic capture of beads on a sensor to provide diverse real-time or near real-time analysis of analytes.
  • the invention is directed to a magnetic immunosensing device for detecting an analyte in a sample, comprising: a sensing electrode on a substantially planar chip, wherein the electrode is positioned in a conduit for receiving a sample mixed with magnetically susceptible beads; and a high-field magnet, e.g., a permanent magnet or an electromagnet, positioned proximate to said chip for attracting the beads in the conduit substantially proximate to said electrode and substantially retaining the beads at said electrode surface during removal of the unbound sample and washing of said electrode, wherein the beads are coated with an antibody to an analyte in the sample.
  • a high-field magnet e.g., a permanent magnet or an electromagnet
  • Another embodiment of the present invention is directed to a method of performing a sandwich immunoassay for an analyte in a sample with a magnetic imniunosensor, wherein said immunosensor comprises a sensing electrode on a substantially planar chip, wherein the electrode is positioned in a conduit for receiving a sample mixed with magnetically susceptible beads, and a high-field magnet, e.g., a permanent magnet or an electromagnet, positioned proximate to said chip, said method comprising: (a) mixing magnetically susceptible beads coated with a capture antibody with a sample containing an analyte and a signal antibody to form a sandwich on the beads; (b) applying the mixture to the immunosensor; (c) magnetically localizing and retaining at least a portion of the beads on the electrode; (d) washing the unbound sample from the electrode; (e) exposing the signal antibody of the sandwich to a signal generating reagent; and (f) measuring a signal from the reagent at the electrode.
  • said immunosensor comprises
  • a method of performing a competitive immunoassay for an analyte in a sample with a magnetic immunosensor wherein said immunosensor comprises a sensing electrode on a substantially planar chip, wherein the electrode is positioned in a conduit for receiving a sample mixed with magnetically susceptible beads, and a high-field magnet, e.g., a permanent magnet or an electromagnet, positioned below said chip, is provided.
  • a high-field magnet e.g., a permanent magnet or an electromagnet
  • This method comprises: (a) mixing magnetically susceptible beads coated with a capture antibody with a sample containing an analyte and a labeled form of said analyte to permit competitive binding on the beads; (b) applying the mixture to the immunosensor; (c) magnetically localizing and retaining at least a portion of the beads on the electrode; (d) washing the unbound sample from the electrode; (e) exposing the localized analyte to a signal generating reagent; and (f) measuring a signal from the reagent at the electrode.
  • kits for performing an analyte immunoassay in a whole blood sample comprising: magnetically susceptible beads; a sensing electrode on a substantially planar chip; and a high-field magnet, e.g., a permanent magnet or an electromagnet, positioned proximate to the chip for attracting the beads substantially proximate to the electrode and substantially retaining the beads at the electrode surface.
  • a high-field magnet e.g., a permanent magnet or an electromagnet
  • FIG. 1 is a cross-sectional illustration of a grooved immunosensor chip in a conduit with applied magnetic fields in accordance with one embodiment of the present invention
  • FIG. 2 is a top view of the grooved immunosensor chip and a microelectrode array
  • FIGS. 3A-C illustrate various exemplary configurations for the positioning of a rare earth permanent magnet below an immunosensor chip within a cartridge
  • FIG. 4 is a schematic of magnetic field lines for sensor configurations
  • FIG. 5 is a micrograph of magnetically susceptible beads captured on a chip surface where the center of the magnet is positioned directly below the perimeter of an immunosensor;
  • FIG. 6 illustrates three traces of the electrochemical detection step for the magnetically susceptible bead capture assay: plasma with zero cTnl; Cliniqa control level 3; and Cliniqa control level 4.
  • FIG. 7 illustrates the positioning of a rare earth permanent magnet below an immunosensor chip within a cartridge housing in accordance with one embodiment of the present invention
  • FIG. 8 illustrates a foldable cartridge housing in accordance with one embodiment of the present invention where the rare earth permanent magnet is positioned underneath an immunosensor chip;
  • FIG. 9 is a micrograph of magnetically susceptible beads localized on a patterned magnetic layer of PVA and NdFeB particles;
  • FIG. 10 is a micrograph of a fractured cross-section of the device of FIG. 9;
  • FIGS. 1 lA-C include micrographs of a patterned PVA film with various particle sizes of NdFeB (FIG. 11 A); ground 6 ⁇ MOP in polyimide (FIG. 11B); ground 6 ⁇ MOP in polyimide(FIG. 11C);
  • FIG. 12A is a micrograph of a 6 ⁇ MQP NdFeB powder and FIG. 12B is a micrograph of the 6 ⁇ MQP NdFeB powder comminuted using a ball mill;
  • FIG. 13 is a micrograph of beads captured on NdFeB particle surfaces
  • FIGS. 14A and 14B show exemplary base immunosensor electrode arrays partially covered with a printed NdFeB magnetic layer leaving a portion of the perimeter of the array exposed;
  • FIGS. 15A and 15B depict the over-printed magnetic layer in accordance with other embodiments of the present invention.
  • FIG. 16 is a sheared sensor illustrating the printed magnetic layer profile of FIGS. 15A and 15B;
  • FIG. 17 illustrates the etched trench process in accordance with one embodiment of the present invention
  • FIG. 18 is a top view of an exemplary underside trench design etched into a silicon wafer
  • FIG. 19 is the cross-sectional profile of the underside trench
  • FIGS. 20A and 20B depict different views of the etched trench
  • FIG. 21 shows the etched trench filled with NbFeB powder in a polyimide resin
  • FIGS. 22 A and 22B are micrographs of a rectangular trench produced on a silicon substrate via reactive ion etching: FIG. 22A shows a cross-section of the trench and FIG. 22B shows a different cross-section of the trench filled with NbFeB powder in a polyimide resin;
  • FIG. 23 depicts an exemplary combined sensor design for an expanded detection range where the magnetic zone is comprised a screen printed line of NdFeB powder in a polyimide matrix;
  • FIG. 24 depicts an exemplary combined sensor design for an expanded detection range where the magnetic zone is comprised of a bulk NdFeB magnet
  • FIG. 25 depicts another combined sensor design in accordance with one embodiment of the present invention where the open circles on the chip are potential print locations for the reagents of the present invention
  • FIG. 26 is a schematic of an oscillating bead immunoassay (OBIA) with a central immiinosensor flanked by two adjacent magnetic zones with the small bead moving in-between in accordance with one embodiment of the present invention
  • FIG. 27 is a schematic illustrating the degree of bead capture with time
  • FIG. 28 shows a multiplexed OBIA where several different types of analyte-capturing beads are present, but where they are effectively separated onto their individual capture sites;
  • FIG. 29 illustrates the comparative principle of an amperometric immunoassay for determination of troponin I (Tnl), a marker of cardiac injury;
  • FIG. 30 is an isometric top view of an immunosensor cartridge cover of one embodiment of the invention.
  • FIG. 31 is an isometric bottom view of an immunosensor cartridge cover of one embodiment of the invention.
  • FIG. 32 is a top view of the layout of a tape gasket for an immunosensor cartridge of one embodiment of the invention.
  • FIG. 33 is an isometric top view of an immunosensor cartridge base of one embodiment of the invention.
  • FIG. 34 illustrates an exemplary segment forming means
  • FIG. 35 is a top view of one embodiment of an immunosensor cartridge.
  • FIG. 36 is a schematic view of the fluidics of one embodiment of an immunosensor cartridge. DETAILED DESCRIPTION OF THE INVENTION
  • the present invention relates to an apparatus and its method of use for determining the presence or concentrations of analytes in a liquid sample with a single-use disposable cartridge.
  • the invention is adapted for conducting diverse real-time or near real-time assays of analytes. This invention is particularly relevant to high sensitivity cardiac troponin assays in whole blood samples.
  • the invention relates to the determination of analytes in biological samples such as blood using magnetic electrochemical immunosensors or other ligand/ligand receptor-based biosensors based on a magnetically susceptible bead localization step.
  • the present invention also hereby incorporates by reference in their respective entireties jointly-owned U.S. Pat. Nos. 7,419,821 to Davis et al. and 7,682,833 and 7,723,099 to Miller et al., each of which is referenced above.
  • One notable advantage of the magnetically susceptible bead capture approach of the present invention is in improving the low-end sensitivity of immunoassays where there is a perceived benefit in detection of extremely low levels of a marker of myocardial injury (e.g., cTnl).
  • Further advantages and benefits of the embodiments of the invention described and disclosed herein include but are not limited to ease of use, automation of many, if not all steps, of the analysis and elimination of user induced error in the analysis.
  • Various embodiments of the present invention are directed to a magnetic immunosensing device or immunosensor that includes a base sensor or sensing electrode on a substantially planar chip where the sensing electrode is positioned in a conduit for receiving a sample mixed with beads that can be attracted to a magnet, or respond to a magnetic field.
  • a high-field magnet e.g., a permanent magnet or an electromagnet
  • the immunosensor chip e.g., below
  • This magnetic zone functions to substantially retain the beads at or near the sensing electrode surface during removal of the unbound sample and washing of the electrode.
  • the beads are coated with an antibody to an analyte in said sample, which provides the basis for an immunoassay.
  • a system comprising a reading apparatus or reader and a single-use cartridge containing the magnetic immunosensor and all the other assay components is used to analyze an analyte in a biological sample.
  • the magnetic immunosensor comprises a sensing electrode on a substantially planar chip and has a high-field magnet, e.g., a permanent magnet or an electromagnet, positioned proximate to (e.g., below) or associated with the chip.
  • the magnetic immunosensor of the present invention provides a field of greater than about 0.1 Tesla and has an "event horizon" (as defined herein) that can efficiently draw beads from a range of about 0.05 mm to about 5 mm in the region of the sensing electrode.
  • the high-field magnet e.g., permanent magnet or electromagnet
  • the high-field magnet includes any material that provides a high magnetic field (e.g., greater than about 0.1 Tesla, greater than 0.4 Tesla or greater than 1 Tesla).
  • the magnetic field can be measured, for example, as the remnant field on a substantially flat surface area of a magnet.
  • the preferred material is a neodymium iron boron alloy (NdFeB) alloy, and more preferably Nd 2 Fei 4 B, other materials may be used.
  • NdFeB neodymium iron boron alloy
  • high-field permanent magnets can include ferrite or aluminum nickel cobalt (AINiCo) magnets, which typically exhibit fields of 0.1 to 1 Tesla.
  • High-field permanent magnets comprised of alloys of rare earth elements (e.g., neodymium alloys and samarium cobalt (SmCo) alloys) exhibit fields in excess of 1 Tesla, e.g., greater than 1.2 Tesla or greater than 1.4 Tesla.
  • rare earth elements e.g., neodymium alloys and samarium cobalt (SmCo) alloys
  • Rare earth magnets are generally brittle and also vulnerable to corrosion, and as such these materials are frequently plated or coated to protect them from breaking and chipping.
  • the Curie point of rare earth magnets is substantially above the temperatures encountered in the immunoassay of the present invention, which may be ain in the ambient to about 50°C range, typically thermostated at 37°C for assays in blood.
  • Curie point or “Curie temperature” refers to a characteristic property of a ferromagnetic material.
  • the Curie point of a ferromagnetic material is the temperature above which it loses its characteristic ferromagnetic ability to possess a net (spontaneous) magnetization in the absence of an external magnetic field.
  • the magnetic moments are partially aligned within magnetic domains in ferromagnetic materials.
  • thermal fluctuations increasingly destroy this alignment, until the net magnetization becomes zero at and above the Curie point. Above the Curie point, the material is purely paramagnetic.
  • the high-field magnet comprises an electromagnet in which the magnetic field is produced by the flow of electric current.
  • the electric current may be provided by a reader, in which the immunosensing device is inserted and with which the immunosensing device is in electrical contact.
  • the magnetic immunosensor of some embodiments of the invention comprises a sensing electrode on a substantially planar chip and a bulk permanent high-field magnet positioned proximate to the electrode (e.g., below or on the opposite side of the chip).
  • the bulk permanent high-field magnet is positioned in the housing (e.g., cut out or trench in the plastic cartridge) of the magnetic immunosensing device.
  • the bulk permanent high-field magnet is positioned within the base of the plastic cartridge housing (e.g., non-coplanar with the sensing electrode).
  • the magnet is positioned adjacent to or within the reading apparatus or reader of the immunosensing device.
  • the bulk high-field permanent magnet is substantially cylindrical, having a diameter in the range of about 0.1 mm to about 5 mm and a length of about 0.1 mm to about 5mm, and is positioned to yield an "event horizon" (as defined herein) in the conduit suitable for bead capture within a short period of time (e.g., 1-5 minutes).
  • the conduit generally has a height of about 0.2 mm to about 5 mm and a width of about 0.2 mm to about 5 mm, and either a uniform or non-uniform cross-sectional area.
  • the bulk magnet shape may be in the form of a square, rectangle, oval, flake, pyramid, sphere, sub-sphere, or other shaped form.
  • the method of some embodiments of the invention includes (a) mixing magnetically susceptible beads coated with a capture antibody with a sample suspected of containing an analyte, and a signal antibody to form a sandwich on the beads, (b) applying the mixture to the immunosensor and magnetically localizing and retaining at least a portion of the beads on the immunosensor, (c) washing the unbound sample from the immunosensor, and (d) exposing the signal antibody of the sandwich to a signal generating reagent, and measuring a signal from the reagent at the electrode.
  • the method can use magnetically susceptible beads and signal antibodies dissolved from a dry matrix.
  • the method operationally relies on step (a) occurring in a first portion of a conduit and step (b) occurring in a second portion of a conduit where the sensor is located.
  • a magnetic immu no sensing device which comprises a sensing electrode on a substantially planar chip.
  • the electrode is positioned in a conduit for receiving a sample mixed with antibody-labeled magnetically susceptible beads and a magnetized layer (e.g., microfabricated magnetic layer).
  • the magnetized layer may be included on (e.g., positioned over, directly attached, coated or patterned onto any surface of the chip) or embedded into the chip (e.g., positioned within the chip, integral to the chip). This configuration attracts the magnetically susceptible beads substantially proximate to the electrode and substantially retains them at the electrode during removal of unbound sample and washing of the electrode.
  • the magnetized layer preferably is formed from a mobile magnetic composition, e.g., a slurry, comprising a material capable of sustaining a high-field permanent magnetic field, e.g., a NdFeB alloy, as particles in an immobilization or support matrix (e.g., a polyimide, polyvinyl alcohol (PVA) or thermoplastic equivalent).
  • a mobile magnetic composition e.g., a slurry
  • a material capable of sustaining a high-field permanent magnetic field e.g., a NdFeB alloy
  • an immobilization or support matrix e.g., a polyimide, polyvinyl alcohol (PVA) or thermoplastic equivalent
  • PVA polyvinyl alcohol
  • the mobile magnetic composition has a viscosity ranging from 0.3 to 300,000 CPS, e.g., from 100 to 100,000 CPS or from 1,000 to 10,000 CPS.
  • the magnetic particles in the slurry of certain embodiments of the invention have an average particle
  • the support matrix for fixing the magnetic particles to the wafer.
  • the methods of curing the matrix may be based on a photo-initiated, thermally initiated or chemically initiated process.
  • the immobilization matrix is comprised of other photoformed matrix materials.
  • the slurry can be applied in a variety of locations in or on the immunosensing device (e.g., to the front side or backside of a wafer or chip, electrode, housing, reader, etc.).
  • the high-field permanent magnetic material is applied to the substantially planar chip in a patterned manner (e.g., using a mask).
  • the high-field permanent magnetic material is also applied to a microfabricated sensing electrode.
  • the slurry is applied in a layer below the sensing electrode.
  • the slurry Prior to the application process, the slurry may or may not be magnetized. However, after the deposition step, the magnetic layer preferably is magnetized to provide directionality to the field.
  • the sensing electrode is preferably microfabricated (e.g., an amperometric gold array) on a substantially planar chip (e.g., silicon wafer), as described in the jointly-owned pending and issued patents cited herein (e.g., U.S. Pat. Nos. 5,200,051 and 7,419,821).
  • a substantially planar chip e.g., silicon wafer
  • the biological sample e.g., blood sample
  • the magnetically susceptible beads may be comprised of any material known in the art that is susceptive to movement by a magnet (e.g., permanent magnet or electromagnet) utilized in or in concert with the device of the present invention.
  • a magnet e.g., permanent magnet or electromagnet
  • the terms “magnetic” and “magnetically susceptible” with regard to beads can be used interchangeably.
  • the beads include a magnetic core, which preferably is completely or partially coated with a coating material.
  • the magnetic core may comprise a ferromagnetic, paramagnetic or a superparamagnetic material.
  • the magnetically susceptible beads comprise a ferrite core and an outer polymer coating.
  • the magnetic core may comprise one or more of Fe, Co, Mn, Ni, metals comprising one or more of these elements, ordered alloys of these elements, crystals comprised of these elements, magnetic oxide stnictures, such as ferrites, and combinations thereof.
  • the magnetic core may be comprised of magnetite (Fe 3 0 4 ), maghemite (y-Fe 2 0 3 ), or divalent metal-ferrites provided by the formula Mei -x OFe3+x0 3 where Me is, for example, Cu, Fe, Ni, Co, Mn, Mg, or Zn or combinations of these materials, and where x ranges from 0.01 to 99.
  • Suitable materials for the coating include synthetic and biological polymers, copolymers and polymer blends, and inorganic materials.
  • Polymer materials may include various combinations of polymers of acrylates, siloxanes, styrenes, acetates, akylene glycols, alkylenes, alkylene oxides, parylenes, lactic acid, and glycolic acid.
  • Biopolymer materials include starch or similar carbohydrate.
  • Inorganic coating materials may include any combination of a metal, a metal alloy, and a ceramic. Examples of ceramic materials may include hydroxyapatite, silicon carbide, carboxylate, sulfonate, phosphate, ferrite, phosphonate, and oxides of Group IV elements of the Periodic Table of Elements.
  • the magnetic beads comprise non- magnetic substrate beads formed, for example, of a material selected from the group consisting of polystyrene, polyacrylic acid and dextran, upon which a magnetic coating is placed.
  • any correctly- sized magnetically susceptible bead capable of being positioned with the high-field magnet of the present invention may be utilized, taking into account the dispersability requirements for the magnetically susceptible beads.
  • at least 50 wt.%, e.g., at least 75 wt.%, of the magnetically susceptible beads are retained at the electrode surface.
  • the average particle size of the magnetically susceptible beads may range from 0.01 ⁇ to 20 ⁇ , e.g., from 0.1 ⁇ to 10 ⁇ , from 0.1 ⁇ to 5 ⁇ or from 0.2 ⁇ to 1.5 ⁇ .
  • the term "average particle size” refers to the average longest dimension of the particles, e.g., beads, for example the diameter for spherical particles, as determined by methods well-known in the art.
  • the particle size distribution of the magnetically susceptible beads preferably is unimodal, although polymodal distributions may also be used in accordance with the present invention. While use of a spherical magnetically susceptible bead is preferred, in other embodiments, other bead shapes and staictures, e.g., ovals, sub-spherical, cylindrical and other irregular shaped particles, are within the meaning of the term “beads” and "microparticles" as used herein.
  • Magnetically susceptible bead preparations include Invitrogen (Carlsbad, California, U.S.A.) by Life Technologies , Ademtech (Pessac, France), Chemicell GmbH (Berlin, Germany), Bangs Laboratories, Inc.TM (Fishers, IN) and Seradyn, Inc. (Indianapolis, IN).
  • Many of the commercially available products incorporate surface functionalization that can be employed to immobilize antibodies (e.g., IgG) on the bead surfaces.
  • Exemplary functionalizations include carboxyl, amino or streptavidin-modified magnetically susceptible beads.
  • the magnetically susceptible beads are preferably coated with an antibody to an analyte that is a cardiovascular marker, e.g., cardiac troponin I, troponin T, a troponin complex, human chorionic gonadotropin, BNP, creatine kinase, creatine kinase subunit M, creatine kinase subunit B, creatine kinase MB (CK-MB), proBNP, NT-proBNP, myoglobin, myosin light chain or modified fragments thereof, among others.
  • markers for other indications can be utilized.
  • analytes include, but are not limited to, beta-HCG, TSH, ultra hTSH II, TT3, TT4, FT3, FT4, myeloperoxidase, D-dimer, CRP, NGAL, PSA, LH, FSH, galectin-3, prolactin, progesterone, estradiol, DHEA-S, AFP, CA 125 II, CA 125, CA 15-3, CA 19-9, CA 19-9XR, CEA, thyroxine (T4), triiodothyronine (T3), T-uptake, Tg, anti-Tg, anti-TPO, ferritin, Cortisol, insulin, HBsAg, HCV Ag/Ab combo, HCV core Ag, anti-HCV, AUSAB (anti-HBs), CORE, CORE-M, SHBG, iPTH, theophylline, sirolimus, tacrolimus, anti-HAV, anti-HAV Ig
  • the magnetic immunosensor and methods of the present invention preferably also comprise a second antibody, which is a labeled antibody, also referred to herein as a signal antibody.
  • the labeled antibody is in the form of a dissolvable dry reagent, which also may comprise the magnetically susceptible beads that are employed in the present invention, as discussed below.
  • Both the immobilized and labeled antibodies can be monoclonal, polyclonal, fragments thereof and combinations thereof.
  • one or more of the antibodies can be labeled with various labels including a radiolabel, enzyme, chromophore, flurophore, chemiluminescent species, ionophore, electroactive species and others known in the immunoassay art.
  • the second antibody is labeled with an enzyme
  • it is preferably ALP, horseradish peroxidase, or glucose oxidase.
  • the analyte is labeled with fluorescein, ferrocene, p-aminophenol, or derivatives thereof.
  • the magnetically susceptible beads are deposited in a suitable region of the magnetic imniunosensing device as a suspension in, for example, a mixture of lactitol and DEAE-dextran such as that supplied by Advanced Enzyme Technologies (Pontypool, Great Britain). Evaporation of the solvent, usually water, yields a glassy deposit in which the beads are immobilized.
  • the lactitol/DEAE-dextran allows the beads to be regionalized within the device in a mechanically and biochemically stable state, but which also rapidly dissolves upon contact with a sample.
  • the magnetically susceptible beads are homogeneously mixed with the sample.
  • the magnetically susceptible beads may be less homogeneously mixed with the sample; however, the intent is to optimize the position and concentration of the beads relative to the sensing electrode.
  • the magnetically susceptible beads of the present invention may be added to the biological sample prior to introduction into the magnetic imniunosensing device, such as, for example, as an integral part of a blood collection device or as a standard manual addition step. However, for the convenience of the user and to assure a quality assay, the magnetically susceptible beads are preferably included within the device.
  • the sample e.g., whole blood sample
  • a dry reagent comprising the magnetically susceptible beads into the sample.
  • the dry reagent e.g., sensing device, conduit, sample entry port, sample holding chamber.
  • the dry reagent may further include one or more of: beads for reducing leukocyte interference, a leukocidal reagent, buffer, salt, surfactant, stabilizing agent, simple carbohydrate, complex carbohydrate and various combinations thereof.
  • the dry reagent can also include an enzyme-labeled antibody (e.g., the above-described labeled antibody) to the analyte.
  • the magnetically susceptible beads are used to amend the biological sample, e.g., blood, in a first container or location, and then the sample is passed to a second container or location that includes the capture and signal antibodies.
  • the magnetically susceptible beads are contained in solution and mixed with the biological sample, and the resulting amended sample is introduced into the magnetic imniunosensing device.
  • a blood sample may be mixed with the magnetically susceptible beads to form an amended sample, which is then introduced into the device.
  • the magnetic immunosensing device e.g., cartridge
  • the magnetic immunosensing device includes a pouch that contains a liquid comprising the magnetically susceptible beads, which may be mixed with a biological sample in the device and then processed substantially as described herein to form an assay (e.g., sandwich assay) for analyte detection.
  • an assay e.g., sandwich assay
  • electrowetting is employed to mix a first liquid comprising the magnetically susceptible beads with a liquid biological sample, e.g., blood.
  • an apparatus may be provided for manipulating droplets.
  • the apparatus may have a single-sided electrode design in which all conductive elements are contained on one surface on which droplets are manipulated.
  • an additional surface is provided parallel with the first surface for the purpose of containing the droplets to be manipulated.
  • the droplets are manipulated by performing electrowetting-based techniques in which electrodes contained on or embedded in the first surface are sequentially energized and de-energized in a controlled manner.
  • the apparatus may allow for a number of droplet manipulation processes, including merging and mixing two droplets together, splitting a droplet into two or more droplets, sampling a continuous liquid flow by forming from the flow individually controllable droplets, and iterative binary or digital mixing of droplets to obtain a desired mixing ratio.
  • any immunoassay format known in the art may be modified to include the magnetically susceptible beads of the present invention, for example, by adding the beads in a sample pre-treatment step.
  • the pretreatment may be accomplished, for example, by incorporating the beads in a blood collection device, in a separate vessel, or may take place in the immunoassay device itself by incorporation of the beads in the test cycle of the device.
  • the beads are mobile and thereby capable of interacting with an analyte. After binding to the analyte of interest, magnetic forces are used to concentrate the beads at the electrode for measurement causing the magnetically susceptible beads to be localized to the ampero metric electrode for signal detection.
  • One advantage of using mobile beads according to the present invention is that their motion in the sample or fluid accelerates binding reactions, making the capture step of the assay faster.
  • additives may be included in the magnetic immunosensing device or used in conjunction with the assay.
  • an anticoagulant can be added.
  • heparin may be added to improve performance in cases where the sample was not collected in a heparinized tube or was not properly mixed in a heparinized tube.
  • a sufficient amount of heparin may be added so that fresh unheparinized blood will remain uncoagulated during the assay cycle of the cartridge, typically in the range of 2 to 20 minutes.
  • one or more of proclin, DEAE-dextran, tris buffer, and lactitol can be added as reagent stabilizers.
  • a surfactant such as polysorbate 20, also known as Tween® 20, can be added to reduce binding of proteins to plastic, which is a preferred material for the cartridge housing of the magnetic immunosensing device.
  • a surfactant also facilitates the even coating of reagents on plastic surfaces and minimizes the cry stallization of sugars (e.g., lactitol).
  • an antibacterial agent or biocide e.g., sodium azide
  • a silicon wafer is thermally oxidized to form an insulating oxide layer having a thickness of about 1 um.
  • a titanium/tungsten layer is then sputtered onto the oxide layer to a preferable thickness of about 100 A to about 1000 A, followed by a layer of gold that is from 500 A to 1000 A thick, most preferably about 800 A thick.
  • a photoresist is spun onto the wafer and is dried and baked. The surface is then exposed using a contact mask, the latent image is developed, and the wafer is exposed to a gold-etchant.
  • the patterned gold layer is coated with a pliotodefinable polyimide, suitably baked, exposed using a contact mask, developed, cleaned in an oxygen plasma, and preferably imidized at 350°C for about 5 hours.
  • the square array has a diameter, for example, from 2 ⁇ to 100 ⁇ , from 5 ⁇ to 15 ⁇ or about 7 ⁇ , with an inter-distance of, for example, from 5 ⁇ to 100 ⁇ , from 10 ⁇ to 20 ⁇ or about 15 ⁇ .
  • the area covered by these electrodes i.e., sensor area
  • each chip is assembled into a single-use cartridge.
  • the cartridges may be of the type described in U.S. Pat. Nos. 7,419,821 to Davis et al. or jointly-owned U.S. Patent Application No. 61/288, 189, entitled "Foldable Cartridge Housings for Sample Analysis,” filed December 18, 2009, the entireties of which are incorporated herein by reference.
  • the sensor is positioned in a conduit for receiving a sample, and a high-field magnet, e.g., permanent or electromagnet, is positioned directly below the sensor, preferably in the center region thereof.
  • a high-field magnet can be positioned above the sensor region of the conduit.
  • the one or more high-field magnets can be used for attracting magnetically susceptible beads in the conduit (e.g., substantially proximate to the sensor) and retaining them in the region of the sensor during removal of sample and washing of the sensor to remove unbound or partially absorbed reagents.
  • the magnetic beads are coated with an antibody to an analyte that may be present in the sample.
  • FIG. 1 shows an electrochemical sensor patterned in suitable material (e.g., photoformable polyimide) with trenches (or grooves) having width of from about 1 um to about 100 um, e.g., from 3 ⁇ to 50 ⁇ or from 10 ⁇ to 20 ⁇ , height or depth of from about 0.1 ⁇ to about 100 ⁇ , e.g., from 1 ⁇ to 50 ⁇ or from 5 ⁇ to 10 ⁇ , and length of about 1 ⁇ to about 5000 ⁇ , e.g., from 10 ⁇ to 1000 ⁇ or from 100 ⁇ to 500 ⁇ .
  • suitable material e.g., photoformable polyimide
  • the planar chip is positioned into an analytical system capable of applying magnetic fields in either direction in roughly the z axis, as shown by vectors Bl and B2.
  • the trenches may be oriented in a direction substantially parallel to the direction of sample flow in the immunosensing device.
  • the trenches may be oriented in a direction substantially transverse to or perpendicular to the direction of sample flow.
  • the trench structure of the present invention may beneficially inhibit fluid motion in the x and/or y directions from removing any magnetically susceptible beads that have been magnetically localized within a trench, for example, by a washing fluid.
  • FIG. 2 A top view illustration of the electrochemical sensor of FIG. 1 is illustrated in FIG. 2, in which microelectrodes of diameter ⁇ (i.e., about 1 um to about 100 u ) and spacing ⁇ . (about 5 ⁇ to about 500 ⁇ ) are observed at the bottom of each groove in the sensor staicture as an array.
  • These microelectrodes can be comprised of gold or other suitable conductor and can be patterned as described above (e.g., with gold and polyimide).
  • the high-field magnet is positioned on the underside or below the electrochemical sensor.
  • a sandwich immunoassay is formed on the magnetically susceptible beads substantially everywhere in the conduit and the beads are slowly collected on or adjacent to a sensor surface by bringing the magnet proximal to the cartridge and slowly oscillating the fluid.
  • the oscillating magnetic fields are produced by a moveable high-field magnet, e.g., permanent magnet or electromagnet, located inside the instalment.
  • one or more magnets are stationary inside the instalment and the sandwich formation is located in a position beyond the field.
  • the sample may then be directed, e.g., by a pump or similar means, into a region where the magnetic field is sufficiently strong for capture.
  • the sample is oscillated in a back and forth motion over the sensor, e.g., by one or more pumps, while the magnetic field is applied, in order to maximize the opportunity for the magnetically susceptible beads to be attracted to the sensor surface.
  • the present invention is applicable to methods of performing immunoassays with a magnetic immunosensor.
  • the present invention may be employed in one or more of the following areas: immunosensors, most notably in the context of point-of-care testing; electrochemical immunoassays; immunosensors in conjunction with immuno-reference sensors; whole blood immunoassays; single-use cartridge based immunoassays; and nonsequential immunoassays with only a single wash step; and dry reagent coatings.
  • immunosensors most notably in the context of point-of-care testing
  • electrochemical immunoassays immunosensors in conjunction with immuno-reference sensors
  • whole blood immunoassays single-use cartridge based immunoassays
  • nonsequential immunoassays with only a single wash step and dry reagent coatings.
  • the general concept disclosed herein is applicable to many immunoassay methods and platforms.
  • the methods of the invention are applicable to various biological sample types (e.g., blood, plasma, seaim, urine, interstitial fluid and cerebrospinal fluid).
  • the present invention is applicable a variety of immunoassays including both sandwich and competitive immunoassays.
  • the sample contacts the immunosensor with an immobilized first antibody to the target analyte, and a labeled second antibody to said target analyte.
  • the sample e.g., whole blood sample
  • the sample is collected and then amended by dissolving a dry reagent comprising magnetically susceptible beads into the sample.
  • the beads preferably include an antibody to the analyte of interest immobilized on the outer surface thereof. The dissolution of the dry reagents and the sandwich formation step can occur concurrently or in a stepwise manner.
  • the magnetically susceptible bead concentration employed may vary widely.
  • the sample is amended with the magnetically susceptible beads to provide a dissolved bead concentration of at least 5 ⁇ g per of sample, e.g., at least 10 ⁇ g per ⁇ of sample, or at least 15 ⁇ g per ⁇ , of sample.
  • the dry reagent preferably dissolves into the sample to give a bead concentration of from about 5 ⁇ g to about 40 ⁇ g beads per ⁇ of sample, preferably from about 10 to about 20 ⁇ g beads per ⁇ . of sample. Depending on the size of the beads, this corresponds to at least about 10 4 beads per of sample, at least about 10 3 beads per ⁇ .
  • the beads are present in an amount sufficient to provide a dissolved bead concentration of at least 10 4 beads per ⁇ of sample, e.g., at least about 10 3 beads per ⁇ , of sample, or from about 10 3 to about 10 6 beads per ⁇ of sample.
  • an immunoassay e.g., an electrochemical immunoassay
  • at least about 10,000 beads are used for each assay. This lower limit reduces counting error issues, e.g., about 1% or greater for about 1,000 beads or less. Defining an upper limit is less straightforward and depends on bead size, however about 10,000,000 beads is generally sufficient.
  • the dissolved bead volume is less than about 1% of the total sample assay volume, and is preferably less than about 0.1%.
  • a magnetic field is applied to attract the beads in the conduit substantially proximate to the electrode.
  • the sample is subsequently washed to a waste chamber while leaving the retained beads substantially proximate to the electrode, followed by exposing the sandwich on the magnetically susceptible beads to a substrate capable of reacting with an enzyme to form a product capable of electrochemical detection.
  • a substrate capable of reacting with an enzyme to form a product capable of electrochemical detection.
  • One exemplary format is an electrochemical enzyme-linked immunosorbent assay.
  • a magnetized layer e.g., microfabricated magnetic layer
  • the invention is to a method of performing an immunoassay with a magnetic immunosensor where the immunosensor comprises a sensing electrode on a substantially planar chip and an additional layer on the chip that is magnetized, positioned in the region of the electrode.
  • This method comprises (a) mixing magnetically susceptible beads coated with a capture antibody with a sample containing (or suspected of containing) an analyte and a signal antibody to form a sandwich on the beads, (b) applying the mixture to the immunosensor and magnetically localizing and retaining at least a portion of the beads on the immunosensor, (c) washing the sample and unbound material from the immunosensor and exposing the signal antibody of the sandwich to a signal generating reagent, and (d) measuring a signal from the reagent at the electrode.
  • step (a) preferably occurs in a first portion of a conduit and step (b) preferably occurs in a second portion of a conduit where the sensor resides.
  • other features of the assay e.g., sample type, the use of dry reagents including magnetically susceptible beads and the signal antibodies, as described herein
  • Embodiments of the present invention are also applicable to methods of performing a competitive immunoassay with a magnetic immunosensor.
  • a sample contacts an immunosensor comprising an immobilized first antibody to a target analyte, and a labeled target analyte that competes for binding with the target analyte. Because the (unlabeled) target analyte competes with (amended) labeled target analyte, the resulting signal is inversely proportional to the native analyte concentration of the sample.
  • the method of the invention includes mixing
  • the assay involves sampling of an analyte present in plasma or whole blood by capturing analyte on an antibody-labeled microparticle which is coated (or permanently bound) to the surface of a sensor, e.g. an electrochemical sensor.
  • a sensor e.g. an electrochemical sensor.
  • a second antibody labeled with an enzyme then binds to the analyte to make a sandwich.
  • electrochemical detection format ensures that substantially all the enzyme (e.g., alkaline phosphatase (ALP)) is detected (i.e., detection efficiency approaches 100%), which allows for a high sensitivity assay.
  • ALP alkaline phosphatase
  • detection efficiency approaches 100%
  • the overall amount of analyte that is in the region of the antibody-labeled microparticle and susceptible to capture during normal usage is low due to mass transport limitations. It would be advantageous, therefore, to capture a higher percentage of the analyte, while still retaining the high efficiency of the detection step.
  • more analyte advantageously may be captured and detected by employing capture of a magnetically susceptible bead reagent that is distributed (e.g., homogeneously distributed) throughout the sample during the analyte capture step, but is magnetically localized to the sensor in such a way that retains the ability to measure the enzyme with sufficiently improved detection efficiency.
  • a magnetically susceptible bead reagent that is distributed (e.g., homogeneously distributed) throughout the sample during the analyte capture step, but is magnetically localized to the sensor in such a way that retains the ability to measure the enzyme with sufficiently improved detection efficiency.
  • the sample e.g., plasma or whole blood sample
  • the reagents may contain immunoglobulins, immunoglobulin-coated non-magnetically susceptible beads, non-magnetically susceptible bead reagents for interference screening, and other stabilizing or conditioning reagents. See, e.g., U.S. Publication Nos.
  • the primary capture reagent comprises magnetically susceptible beads coated with antibodies or antibody fragments appropriate to the analyte of interest.
  • This magnetically susceptible bead reagent may be printed, for example, on a wall portion of the holding chamber or attached to a conduit in a region upstream of the sensor chip, using methods described in jointly-owned U.S. Pat. No. 5,554,339 to Cozzette et al, which is hereby incorporated by reference in its entirety.
  • the labeled conjugate reagent preferably also comprises a signaling element (e.g., ALP).
  • This reagent may be printed, for example, as a dissolvable matrix with the primary capture reagent in a one stage assay.
  • ALP signaling element
  • the two reagents are amended into the sample separately from one another, in either order.
  • the amended sample be oscillated or mixed in the conduit for an appropriate period of time (e.g., about 1 minute to about 20 minutes or about 5 to 10 minutes). This allows analyte present in the sample to be captured on the surface of the beads and labeled with a signal-generating conjugate.
  • magnetic field B l is applied in such a way that the magnetically susceptible beads are induced to migrate to the top of the sensor channel, opposite the sensor surface, where they are temporarily retained.
  • the surface i.e., top of sensor channel
  • the surface may be patterned in such a way that there is a tendency of the beads to resist (beyond the action of B l) movement in the direction of mixing upon subsequent fluid movement.
  • This process can be accompanied by low-amplitude oscillations in the direction of the capture motion (x-axis) in order to assist capture of the magnetically susceptible beads without entrapping formed elements or non-magnetically susceptible beads.
  • the sample is then moved to a waste chamber.
  • the sample is moved to a lock-wick feature by means of pressurization actuated by an air-bladder as described in jointly-owned U.S. Pat. No. 7,419,821 to Davis et al. (referenced above) and U.S. Pat. No. 7,723,099 to Miller et al. (referenced above).
  • pressurization actuated by an air-bladder as described in jointly-owned U.S. Pat. No. 7,419,821 to Davis et al. (referenced above) and U.S. Pat. No. 7,723,099 to Miller et al. (referenced above).
  • Each of these patents is hereby incorporated by reference in its entirety.
  • the movement of the magnetically susceptible beads is conducted in such a way that the magnetic field B l is able to temporarily retain the beads at the ceiling of the sensor channel, preferably substantially opposite the sensor.
  • the sensor channel is washed in a fashion similar to that described in U.S. Pat. Nos. 7,419,821 and 7,723,099 (referenced above).
  • a portion of the wash fluid is left in the sensor channel until completion of the following magnetic actuation step.
  • the magnetic field Bl is reversed to B2 with the effect that the beads formerly held in position at the sensor channel ceiling now migrate towards the trenches or grooved sensor structure.
  • Low amplitude oscillations of the fluid portion in the direction of capture mixing can be applied in order to help settle the beads into the trenches of the immunosensor.
  • the wash fluid is slowly pulled from the sensor channel and optionally the magnetic field B2 is turned off.
  • a current arising from the diffusion of an electroactive species generated by the action of bound label on a suitable electrogenic substrate contained in the wash fluid is then measured. (See, for example, U.S. Pat. Nos. 7,419,821 and 7,723,099 (referenced above)).
  • the trenches or grooved structure of the immunosensor are intended to aid capture of the magnetically susceptible beads and yet allow for efficient washing in the direction of fluid movement.
  • Other suitable staictures may also be used.
  • One exemplary structure includes a grid (e.g., rectilinear) array.
  • the ability to retain the beads over the array will generally depend on the ability to focus magnetic fields in such a way that highest field densities are contained within the area demarcated by the area of the array.
  • the beads it is desirable to seek to capture substantially all or a reliable fraction (e.g., over 75 wt.%) of the beads, in which case the dimensions of the bead retention feature (e.g., trenches) are of secondary importance provided that they supply sufficient volume to contain the beads.
  • a fixed proportion of the beads may be sampled in which case the total volume of the capture feature(s) must be held constant.
  • Certain embodiments of the invention utilize sequential application of two opposing magnetic fields. In other embodiments, a single applied field may be utilized.
  • the ability to provide sufficient substrate to the enzyme-limited detection reaction may require high substrate concentrations (e.g., about 20 mM).
  • the number and dimensions of the optional trenches or other retention features are dependent on the size and number of the magnetically susceptible beads required to achieve efficient capture of analyte present in the sample.
  • the length of the trenches may be several thousand microns while the height and width may be on the order of several microns.
  • one function of the trenches is to allow for localization and consolidation of the beads and enhanced resistance against fluid motion to dislodge them.
  • the beads inside the trenches are mobile.
  • each chip after dicing the wafer into individual chips, each chip preferably is assembled into a single-use cartridge and in this example a standard immunosensor is used without the groove features.
  • the sensor is positioned in a conduit for receiving a sample, wherein a single high-field magnet, e.g., permanent magnet or electromagnet, preferably a neodymium iron boron magnet (e.g., Nd 2 Fei 4 B), is also positioned directly below the center of the sensor.
  • the high-field magnet is used for attracting magnetically susceptible beads in the conduit (e.g., substantially proximate to the sensor) and retaining them in the region of the sensor during removal of sample and washing of the sensor.
  • the beads are coated with an antibody to an analyte in the sample or suspected of being present in the sample.
  • FIGS. 3A-C shows three exemplary configurations of the sensor chip or die in a base or cartridge housing of the type described in jointly-owned U.S. Pat. Nos. 7,419,821 and 7,723,099 (referenced above) or U.S. Patent Application No. 61/288, 189 (referenced above).
  • the oval stnicture on the sensor die corresponds to the immunosensor, which is positioned in the conduit.
  • the high-field permanent magnets are cylindrical with lengths of from 1 mm to 10 mm, e.g., from 2 mm to 5 mm, preferably about 3 mm, and diameters of from 0.1 mm to 5 mm, e.g., from 0.5 mm to 2 mm.
  • the magnets have diameters of about 1 mm, about 0.5 mm and about 0.3 mm, respectively.
  • the magnets are abutted to the underside of the chip, which preferably has a thickness of from about 0.2 mm to 5 mm, e.g., from 0.5 mm to 2 mm or preferably about 1 mm.
  • FIG. 4 is a schematic representation of the magnetic field lines as the magnet diameter decreases from 1 mm to 0.3 mm and illustrating how the field and magnet selection may impact where on the sensor the magnetically susceptible beads are attracted and focused.
  • FIG. 5 is a micrograph of magnetically susceptible beads captured on a chip surface where the center of the magnet having a diameter 1 mm is positioned directly below a point on the perimeter of an immunosensor. This configuration assisted with showing the contrast between beads (black), the gold electrode area (white) and the base silicon material (gray). As shown in FIG. 5, a majority of the beads are localized onto the area of the surface directly above the magnet when a suspension of the beads is passed down the conduit and into the region of the magnet for capture. The beads were about 3 ⁇ in diameter.
  • FIG. 6 includes three traces of the electrochemical detection step (chronoamperometry) for the magnetically susceptible bead capture assay: plasma with zero cTnl; Cliniqa control level 3; and Cliniqa control level 4.
  • bitmap curves were run in substantially the same way as a commercial cTnl cartridge, but with special software for the new fluidic motions. All the reagents for this particular experiment were printed on the sensor chip.
  • the cTnl levels L3 and L4 were about 1 1 ng/niL and 40 ng/niL respectively.
  • Each trace shows the current at the electrode as a function of time.
  • the rise time of the current to a steady-state value reflects the time constant (TC) for the sensor and the plateau value reflects the amount of analyte in the sample.
  • the traces in FIG. 6 show only the detection step, the complete assay took about 12 minutes, which is acceptable for quantitative point of care immunoassays.
  • Embodiments of the present invention demonstrate that using a fixed permanent magnet in the present device can reliably capture beads (e.g., superparamagnetic or
  • FIG. 7 illustrates the positioning of a rare earth permanent magnet R21 below an immunosensor chip within a cartridge housing in accordance with one embodiment of the present invention
  • FIG. 8 illustrates a foldable cartridge of the type described in previously referenced U.S. Patent Application No.
  • an immunosensor is provided where the magnetic component is directly integrated into sensor manufacture, rather than being a separate component (e.g., bulk permanent high-field magnet) requiring assembly into the test device.
  • a mixture of photoforiiiable polyvinyl alcohol (PVA) mixed with ground Nd 2 Fe 14 B powder was printed onto a wafer using a niicrodispensing apparatus of the type described in jointly-owned U.S. Pat. No. 5,554,339 (referenced above).
  • FIG. 9 is a micrograph of a portion of the printed area where the relatively smaller ferromagnetic (dark) beads are accurately localized on the patterned magnetic layer. As shown, small areas of the relatively larger and irregular NdFeB (light) particles are observable below the bead s.
  • FIG. 10 is a micrograph of a fractured cross- section of the device in FIG. 9.
  • FIGS. 1 lA-C illustrate details of the patterned PVA and polyimide films with various particle sizes of NdFeB (FIG. 10A); ground 6 ⁇ Magnaquench particles (MQP) in polyimide (FIG. 10B); and ground 6 ⁇ MQP in polyimide (FIG. IOC), before exposure to the
  • MQP Magnaquench particles
  • SPR Shipley Photo Resist
  • the about 3 ⁇ to about 7 ⁇ thick magnetic film of FIG. 9 was partially less effective in terms of capturing the beads than the about 30 ⁇ to about 40 ⁇ thick films of FIGS. 1 lA-C. While the "capture radius" of the embodiment of FIG. 9 was several tens of microns, the capture radius of the embodiment of FIGS. 1 lA-C was at least 200 ⁇ . In preferred embodiments, the average capture radius of the sensors is less than about 500 ⁇ , e.g., less than 300 ⁇ or less than 250 ⁇ .
  • the shape and position of the magnet as well as its composition aid in the sensitivity and precision of assays using the techniques of the present invention.
  • FIG. 12A is a micrograph of a MQP NdFeB powder with an average particle size of about 6 ⁇ .
  • FIG. 12B is a micrograph of the MQP NdFeB powder of FIG. 12A that has been comminuted via ball milling for about 3 days.
  • the communited NdFeB powder provides for a more homogeneous mobile magnetic composition, which may facilitate magnetic capture of the beads.
  • FIG. 13 is a micrograph of beads captured on NdFeB particle surfaces.
  • FIGS. 14A and 14B show exemplary base immunosensor electrode arrays 110 partially covered with a printed polyimide and NdFeB particle matrix 1 11 leaving a portion of the perimeter of the array exposed. Also shown is the base silicon wafer 112 and a conductive line 1 13 for connecting the array to the instalment electronics.
  • FIG. 16 is a sheared (i.e., fractured) sensor illustrating the printed magnetic layer profile of FIGS. 15A and 15B.
  • the high-field magnet e.g., permanent or electromagnet
  • the high-field magnet be within a few tens of microns of the amperometric sensor electrode surface in order to speed up the time constant (TC) of the sensor.
  • Effective permanent capture relates to the attraction of a magnetic particle to the magnet, at the point at which the acceleration of the particle is greater than any of the potentially “disaiptive” fluid motions (e.g., mixing oscillations and washing). Physical observation of this phenomenon with a microscope, therefore, can give a practical rough estimate of the "event horizon" for any given bead, sensor design and fluidic motion. Such information is useful in refining the overall test system design to achieve capture of substantially all or a reliable fraction (e.g., over 75 wt.%) of the beads from device to device.
  • One intended use of the present invention is in making single-use disposable test cartridges in large volumes (i.e., greater than a million per year) and each device must perform reproducibly within a given batch, (i.e., have clinically acceptable precision and accuracy).
  • a 10 uL segment of blood sample is oscillated over the immunosensor in a conduit of 0.5 mm height using a prototype design adapted from the i-STAT® immunoassay cartridge format.
  • This process is performed with a 4 second cycle time (i.e., 4 Hertz) and equates to a maximum fluid velocity of about 10 cm per second.
  • the magnetically susceptible beads are observed to move at about 10 um per second in the direction of the magnetic field (microscope, jig and cartridge combination not shown). However, once the beads are within about 50 um of the sensor surface, the beads are within the "event horizon" and become captured on the surface.
  • the beads are no longer subject to the influence of the oscillation (i.e., resuspension of the particle is substantially negligible).
  • the trenched design of one embodiment of the present invention (with the intentional fabrication of a stagnant layer) may facilitate the retention of beads at the surface during this step.
  • one or more intervening air segments are present between the sample and wash fluid in the conduit.
  • the menisci formed by the air segments provide a greater shear force to the captured beads as they pass over the sensor than that generated by fluid oscillation where the beads are in constant contact with the liquid phase. It was also found that the slow passage of the menisci over the sensor surface was more disaiptive than a relatively faster motion.
  • the high-field magnets employed in the present invention e.g., any material that provides a high magnetic field (e.g., greater than about 0.1 Tesla) are preferable for optimizing movement of the magnetically susceptible beads in relation to the sensor.
  • the wash fluid comprises a 0.1 M diethanolamine buffer (pH 9.8), 1 mM MgCl 2 , 1.0 M NaCl, 10 mM 4- aminophenylphosphate and 10 uM NaT
  • the dimensions of the high-field magnet are important in that, generally, if the magnet is too small, either the time needed to capture the magnetically susceptible beads is too long or the stability (i.e., retained capture) during the washing step will degrade the assay performance. In addition, if the magnet is too deep below the sensor plane, or held too far away from the sensor surface, the force of attraction will be reduced and more diffuse and the magnetic reagents are poorly focused upon capture. Based on the present disclosure, those skilled in the art will understand how to optimize these various requirements for any given system and geometry (e.g., electrode area and position in a conduit).
  • the magnet diameter or width affects the time constant (TC) of the sensor and how effectively the sensor detects the signal-generating enzyme labels bound to the magnetic reagents on the magnet surface.
  • FIG. 17 illustrates the etched trench process in accordance with one embodiment of the present invention, wherein a silicon wafer 1301 with a surface coating of photoresist 1302 is etched first with hydrofluoric acid (HF) and then with hot potassium hydroxide (KOH) or trimethyl ammonium hydroxide (TMAH) to leave a trench of controlled dimensions 1303 (e.g., a depth and width of from about 5 ⁇ to about 200 ⁇ ).
  • a slurry of magnetizable particles e.g., NdFeB powder
  • a thermoplastic matrix e.g., polyimide
  • the wafer 1301 may be further processed, as described in jointly-owned U.S. Pat. Nos. 7,419,821 and 7,723,099 (referenced above), to provide an immunosensor array over each etched trench on a wafer.
  • FIG. 18 is a top view of an exemplary underside trench design etched into a silicon wafer using a 800 ⁇ x 1000 ⁇ mask.
  • FIG. 19 is the after etch cross-sectional profile of the underside trench, having a depth of 50-90% into the silicon wafer.
  • FIGS. 20A and 20B depict different views of the etched trench in accordance with one embodiment of the present invention and FIG. 21 depicts the trench filled with NbFeB powder in a polyimide resin.
  • FIGS. 22A and 22B are micrographs of a rectangular trench produced on a silicon substrate via reactive ion etching by INO (Hamilton, Ontario).
  • FIG. 22A shows a cross-section of the trench while FIG. 22B shows a different cross-section of the trench filled with NbFeB powder in a polyimide resin.
  • the NbFeB powder was about 6 ⁇ in diameter.
  • the silicon wafer is polished on both sides and a trench is etched on one side and filled with magnetic particle material in a binder matrix.
  • Sensor manufacturing processes in accordance with U.S. Pat. Nos. 7,419,821 and 7,723,099 (referenced above) can then be performed on the other side of the wafer.
  • This approach has the advantage of starting the electrode part of the sensor processes on a pristine flat surface.
  • the binder matrix deposition step of the magnetic zone can optionally be performed as the last step in the overall process.
  • a current commercial cTnl assay e.g., i-STAT cTnl cartridge
  • a current commercial cTnl assay can be modified to include a separate but analytically- integrated magnetic capture immunosensor.
  • the prior art sensor covers the detection range of 0.20 ng/mL to 36.00 ng/mL, while certain embodiments of the magnetic capture immunosensor of the present invention cover a lower, but overlapping range of 0.002 ng/mL to 1.0 ng mL.
  • FIG. 23 where the magnetic zone is comprised a screen printed line of NdFeB powder in a polyimide matrix. Another embodiment is shown in FIG.
  • FIG. 24 depicts yet other exemplary combined sensor design where the open circles on the chip are potential print locations for the reagents of the present invention.
  • the crossover from primary use of the magnetic sensor to the standard sensor enables a broad analytical range (e.g., from about 50 ng/mL to about 0.001 ng/mL for cTnl).
  • the use of the overlapping range sensors will include an instalment error detection software protocol, which enables an inconsistency between the cTnl sensor signals to be detected.
  • an instalment error detection software protocol which enables an inconsistency between the cTnl sensor signals to be detected.
  • both sensors should yield elevated signals, while in the absence of cTnl, both sensors should yield relatively low amperometric signals. Failure of these conditions being detected by the operating software would indicate that the analytical result is unreliable. Consequently, the instalment would suppresses reporting a result and instead indicate to the user that the test should be re-ain with a new cartridge.
  • the stabilization of the reagents and mode of printing enables a quick curing matrix.
  • Print cocktails for the enzyme conjugate include, but are not limited to the enzyme-labeled antibody to the analyte in a protein stabilizing matrix of less than 30% solids and more preferably 10% or lower solids.
  • the printed magnetic materials of the present invention cure rapidly (i.e., in less than about 7 days). This relatively quick curing time has the advantage of simplifying the manufacturing process, where a short delay between chip manufacture and assembly into a cartridge is desirable.
  • One oscillating magnetic field embodiment makes critical use of labeled magnetically susceptible beads containing both a label antibody, and an enzyme or fluorescent marker or any other detectable label, termed herein as a "signaling moiety.”
  • the label may be detected by any means known in the art including simple microscopy or a reflectance measurement for the beads attached to the capture site using an immunosensing device.
  • the label can be measured optically through a simple optical density measurement on the capture regions or electrochemically, e.g., through an enzymatic reaction. Additional detection techniques include optical resonators, nuclear magnetic resonance (NMR), piezoelectric, pyroelectric, fluorescence, chemiluminescence and surface acoustic wave, among others.
  • Another embodiment of the invention is to a method of performing a sandwich immunoassay for an analyte in a sample with an immunosensor on a substantially planar surface using a means for applying an oscillating magnetic field (e.g., an electromagnet and/or a moving fixed magnet with respect to the surface of the immunosensor).
  • the first step comprises mixing the magnetically susceptible beads with a sample containing or suspected of containing an analyte, wherein the beads are coated with an antibody to the analyte and a signal generating moiety.
  • the second step requires oscillating the beads across the surface of the immunosensor coated with a second antibody to the analyte, using one of the magnetic means mentioned above. An antibody sandwich is formed thereby immobilizing the beads on the immunosensor.
  • the sample is then washed from the immunosensor, and the signaling moiety on the immunosensor is detected.
  • Various embodiments of the invention are directed to use of the movement of the magnetically susceptible beads along a surface to accelerate the signal generation of an immunoassay.
  • the beads are initially located in a region between two spaced magnetic zones and can move freely between the two. Oscillation of the magnetic field may start slowly and increase in frequency and the magnetically susceptible beads are forced to move back and forth across a surface as the field changes. Through this motion, the beads can be captured in the intervening space on a capture area of the immunosensor having capture antibodies for a particular target analyte.
  • the beads are labeled with analyte specific antibody or antibodies.
  • FIG. 26 is a schematic of an oscillating bead immunoassay (OBIA) with a central immunosensor flanked by two adjacent magnetic zones with the magnetically susceptible beads moving there between, and FIG. 27 shows the enhanced degree of bead capture with time in accordance with one embodiment of the present invention.
  • OBIA oscillating bead immunoassay
  • FIG. 27 shows the enhanced degree of bead capture with time in accordance with one embodiment of the present invention.
  • These immunoassay embodiments are amenable to multiplexing, i.e., testing for more than one type of analyte, because the capture event is localized by the capture immunosensor' s specificity to a particular analyte.
  • FIG. 28 illustrates a multiplexed OBIA where several different types of analyte-capturing beads are present, but where they are effectively separated onto their individual capture sites (e.g., separate immunosensors).
  • the format of the OBIA can be multiplexed with the specific capture regions for the different analytes to be measured being arranged sequentially between the two magnetic concentration areas.
  • a reference sensor (not shown) is also readily applied in this format by having a non-specific antibody layer with the series of specific analyte capture sites.
  • the magnetically susceptible beads are prepared containing label antibodies thereon and are mixed together in controlled ratios, depending on the analytical considerations of the particular assays within the multiplexed immunoassay cartridge. All of the labeled magnetically susceptible beads will then move between the two magnet contact zones across each of the specific capture regions.
  • the different analyte-specific magnetically susceptible beads may have the same label type or, more preferably, will have different types of labels.
  • the different analyte-specific magnetically susceptible beads can be mixed into the same sample and allowed to react prior to their attraction to the sensor surface via the positioning of the magnets.
  • the oscillation of the beads is kept to one side of the capture region for a period of time in order to allow analyte capture on the immiinosensor surface.
  • a further refinement includes a reference sensor to determine the portion of the non-specific signal generated during the analyte capture part of the assay.
  • the magnetic field is preferably actuated via a coil around two ends of a tube or conduit, similar to, for example, the formation of NMR shim fields. This allows control of the force the magnetically susceptible beads experience in contacting the surface of the sensor and minimizes nonspecific binding.
  • the coils have several axes to allow x/y and z motion in the conduit. Fixed magnets on a rotating or oscillating platform may be used in alternative embodiments.
  • FIG. 29 illustrates a comparative amperometric immunoassay for the determination of troponin I (Tnl) 70, a marker of cardiac injury.
  • a blood sample for example, is introduced into the sample holding chamber of the immunosensing device of the present invention, and is amended by a conjugate molecule 71 comprising alkaline phosphatase enzyme (AP) covalently attached to a polyclonal anti-troponin I antibody (cTnl).
  • AP alkaline phosphatase enzyme
  • cTnl polyclonal anti-troponin I antibody
  • This conjugate specifically binds to the Tnl 70 in the blood sample, producing a complex made up of Tnl bound to the AP-aTnl conjugate 72.
  • the blood sample is further amended with polymer beads with a ferrite core 74 coated with a Tnl antibody.
  • the mixture is oscillated in a conduit connected to the holding chamber that generates sandwich formation on the bead.
  • the sensor chip Positioned in the conduit is the sensor chip (or chips), which includes a conductivity sensor used to monitor where the sample is with respect to the sensor chip.
  • the position of the sample segment within the conduit can be actively controlled using the edge of the fluid as a marker. As the sample/air interface crosses the conductivity sensor, a precise signal is generated that can be used as a fluid position marker from which controlled fluid excursions can be executed.
  • the fluid segment is preferentially oscillated edge-to-edge over the sensor.
  • the immunosensor chip is positioned downstream of the mean oscillation position in the conduit.
  • a bulk magnet 76 is positioned under the sensor chip and draws the magnetically susceptible beads to the immunosensor surface.
  • the senor comprises an amperometric electrode used to detect the enzymatically produced 4-aminophenol from the reaction of 4-aminophenylphosphate with the enzyme label alkaline phosphatase.
  • the electrode is preferably produced from a gold surface coated with a photodefined layer of polyimide. Regularly spaced openings in the insulating polyimide layer define a grid of small gold electrodes at which the 4-aminophenol is oxidized in a 2 electron per molecule reaction.
  • Substrates such as p-aminophenol species, can be selected such that the half-wave potential (Ei 2 ) of the substrate and product differ substantially.
  • the Ei 2 of the substrate is substantially higher (i.e., more positive) than that of the product.
  • the product can be selectively electrochemically measured in the presence of the substrate.
  • the concentration of the 4-aminophenylphosphate is selected to be in excess, e.g., 10 times the Km value.
  • the analysis solution is 0.1 M in diethanolamine and 1.0 M NaCl, buffered to a pH of 9.8. Additionally, the analysis solution contains 0.5 niM MgCl, which is a cofactor for the enzyme.
  • a carbonate buffer may
  • the antibodies are selected to bind one or more of protein, e.g., human chorionic gonad otrophin, troponin I, troponin T, troponin C, a troponin complex, creatine kinase, creatine kinase subunit M, creatine kinase subunit B, myoglobin, myosin light chain, or modified fragments thereof.
  • modified fragments are generated by oxidation, reduction, deletion, addition or modification of at least one amino acid, including chemical modification with a natural moiety or with a synthetic moiety.
  • these biomolecules bind to the analyte specifically and have an affinity constant for binding of about 10 7 to lO 13 M "1 .
  • the immunosensor was prepared as follows. A silicon wafer was thermally oxidized to form an insulating oxide layer with a thickness of about 1 ⁇ . A titanium/tungsten layer was sputtered onto the oxide layer to a preferable thickness of about 100 A to about 1000 A, followed by a layer of gold that is most preferably about 800 A thick. A photoresist was then spin coated onto the wafer and was dried and baked. The surface was then exposed using a contact mask, and the latent image was developed. Next, the wafer was exposed to a gold-etchant.
  • the patterned gold layer was coated with a photodefinable polyimide, suitably baked, exposed using a contact mask, developed, cleaned in an O2 plasma, and preferably imidized at 350°C for 5 hours.
  • An optional metallization of the back side of the wafer may be performed to act as a resistive heating element, such as for example in embodiments where the immunosensor is to be used in a thermostatted format.
  • the present example describes a method of using a magnetic immunosensing device in accordance with one embodiment of the invention.
  • an unmetered fluid sample was introduced into sample chamber 34 of a cartridge, through a sample entry port 4.
  • Capillary stop 25 prevents passage of the sample into conduit 1 1 at this stage, and conduit 34 is filled with the sample.
  • Lid 2 is closed to prevent leakage of the sample from out of the cartridge.
  • the cartridge is then inserted into a reading apparatus, such as that disclosed in U.S. Pat. No. 5,821,399 to Zelin (referenced above), which is hereby incorporated by reference.
  • Insertion of the cartridge into a reading apparatus activates the mechanism which punctures a fluid-containing package located at 42 when the package is pressed against spike 38. Fluid is thereby expelled into the second conduit, arriving in sequence at 39, 20, 12 and 11. The constriction at 12 prevents further movement of fluid because residual hydrostatic pressure is dissipated by the flow of fluid via second conduit portion 1 1 into the waste chamber 44.
  • operation of a pump means applies pressure to air-bladder 43, forcing air through conduit 40, through cutaways 17 and 18, and into conduit 34 at a predetermined location 27. Capillary stop 25 and location 27 delimit a metered portion of the original sample.
  • sample chamber 34 While the sample is within sample chamber 34, it is optionally amended with a compound or compounds present initially as a dry coating on the inner surface of the chamber (e.g., antibody-coated magnetically susceptible beads and enzyme-labeled antibody conjugate).
  • a compound or compounds present initially as a dry coating on the inner surface of the chamber e.g., antibody-coated magnetically susceptible beads and enzyme-labeled antibody conjugate.
  • the metered portion of the sample is then expelled through the capillary stop by air pressure produced within air-bladder 43.
  • the sample optionally is oscillated in order to promote efficient sandwich formation on the magnetically susceptible beads.
  • an oscillation frequency of between about 0.2 Hz and about 5 Hz is used, most preferably about 0.7 Hz.
  • the sample is moved forwards along the conduit such that the magnetically susceptible beads can become trapped onto the surface of the magnetic electrode. Subsequently, the sample is ejected from the conduit by further pressure applied to air-bladder 43, and the sample passes to waste chamber 44.
  • a wash step next removes non-specifically bound enzyme-conjugate from the immunosensor area of the conduit. Wash fluid in the second conduit is moved by a pump means 43, into contact with the sensors.
  • the air segment (meniscus) or segments are produced within a conduit by any suitable means, including a passive means, an embodiment of which is shown in FIG. 34 and described in detail in U.S. Pat. No. 7,682,833 (referenced above), or an active means including a transient lowering of the pressure within a conduit using pump means whereby air is drawn into the conduit through a flap or valve.
  • the air segment is extremely effective at clearing the sample-contaminated fluid from conduit 15.
  • the efficiency of the rinsing of the sensor region is greatly enhanced by the introduction of one or more air segments.
  • the leading and/or trailing edges of air segments are passed one or more times over the sensors to rinse and resuspend extraneous material that may have been deposited from the sample.
  • Extraneous material includes any material other than specifically bound analyte or analyte/antibody-enzyme conjugate complex.
  • the washing or rinsing step is not sufficiently protracted or vigorous so as to promote substantial resuspension of the magnetically susceptible beads or dissociation of specifically bound analyte or analyte/ antibody-enzyme conjugate complex from the beads.
  • a further portion of fluid containing the enzyme substrate is placed over the beads on the immu no sensors, and the current or potential, as appropriate to the mode of operation, is recorded as a function of time.
  • FIG. 34 illustrates the constaiction of a specific means for passively introducing an air segment into the sample fluid.
  • recess 140 comprising a tapered portion 141 and a cylindrical portion 142 that are connected.
  • the tapered portion is in fluid connection with a hole of similar diameter in the tape gasket (FIG. 32) that separates the base (FIG. 33) and cover (FIGS. 30 and 31) of the assembled immunosensor cartridge.
  • the recess contains an absorbent material that, upon contact with fluid, withdraws a small quantity of fluid from a conduit thereby passively introducing an air segment into the conduit.
  • the volume of the recess and the amount and type of material within it may be adjusted to control the size of the air segment introduced.
  • Specific absorbent materials include, but are not limited to, glass filter and a laminate comprising a 3 ⁇ Versapor® filter (i.e., acrylic copolymer membrane cast on a nonwoven nylon support) bonded by sucrose to a 60% viscose chiffon layer.
  • a 3 ⁇ Versapor® filter i.e., acrylic copolymer membrane cast on a nonwoven nylon support
  • the cartridge includes a closeable valve, located between the immunosensor and the waste chamber.
  • a blood sample is first introduced into the sample chamber of the cartridge.
  • the diagnostic tests determine whether fluid or sample is present in the conduits using the conductivity electrodes, determine whether electrical short circuits are present in the electrodes, and ensure that the sensor and ground (e.g., reference/counter) electrodes are thermally equilibrated to, preferably, 37°C prior to the analyte determination.
  • a metered portion of the sample preferably between about 4 pL and about 200 pL, more preferably between about 4 pL and about 20 pL, and most preferably about 7 pL, is used to contact the sensor.
  • the edges defining the forward and trailing edges of the sample are reciprocally moved over the conductivity sensor region at a frequency that is preferably between 0.2 to 5.0 Hz, and is most preferably 0.7 Hz.
  • the enzyme-antibody conjugate and magnetically susceptible beads dissolve within the sample.
  • the amount of enzyme-antibody conjugate that is coated onto the conduit is selected to yield a concentration when dissolved that is preferably higher than the highest anticipated cTnl concentration, and is most preferably six times higher than the highest anticipated cTnl concentration in the sample.
  • the sample is moved to the immunosensor for capture of the magnetically susceptible beads.
  • the sample is moved into the waste chamber via closeable valve 41, wetting the closeable valve and causing it to close.
  • the seal created by the closing of the valve 41 permits the first pump means to be used to control motion of fluid from conduit 11 to conduit 15.
  • the analyzer plunger retracts from the flexible diaphragm of the pump mean, creating a partial vacuum in the sensor conduit. This forces the analysis fluid through the small hole in the tape gasket 31 and into a short transecting conduit in the base, 13 and 14.
  • the analysis fluid is then pulled further and the front edge of the analysis fluid is oscillated across the surface of the immunosensor chip in order to shear the sample near the walls of the conduit.
  • the conductivity sensor on the chip is used to control this process.
  • the efficiency of the wash is optimally further enhanced by introduction into the fluid of one or more meniscus or air segment.
  • the air segment may be introduced by either active or passive means. Fluid is then forcibly moved towards sensor chip by the partial vacuum generated by reducing the mechanical pressure exerted upon paddle 6, causing the "T" region of the sensor channel in the vicinity of the transecting conduit to fill with analysis fluid.
  • the T region of the sensor channel optionally has a higher channel height resulting in a meniscus with a smaller radius of curvature. Further away from the T region towards the post-analytical conduit, the conduit height is optionally smaller.
  • the analysis fluid passively flows from the T region towards this low conduit height region, thereby washing the conduit walls.
  • the fluid located within the second conduit also contains a substrate for the enzyme.
  • amendment of the fluid using dried substrate within the second conduit may be utilized.
  • Cartridge 150 comprises a base and a top portion, preferably constaicted of plastic. The two portions are connected by a thin, adhesive gasket or thin pliable film.
  • the assembled cartridge comprises a sample chamber 151 into which a sample containing an analyte of interest is introduced via sample inlet 152.
  • a metered portion of the sample is delivered to the sensor chip 153 (comprising an integrated magnetic layer) via the sample conduit 154 (first conduit) by the combined action of a capillary stop 152, preferably formed by a 0.012" laser cut hole in the gasket or film that connects the two portions of the cartridge, and an entry point 155 located at a predetermined point within the sample chamber whereby air is introduced by the action of a pump means, such as a paddle pushing upon a sample diaphragm 156.
  • a pump means such as a paddle pushing upon a sample diaphragm 156.
  • the wicking material is preferably a cotton fiber material, a cellulose material, or other hydrophilic material having pores. It is important in the present application that the material is sufficiently absorbent (i.e., possesses sufficient wicking speed) that the valve closes within a time period that is commensurate with the subsequent withdrawal of the sample diaphragm actuating means, so that the sample is not subsequently drawn back into the region of the immunosensor.
  • a wash conduit (second conduit) 158 connected at one end to a vent 159 and at the other end to the sample conduit at a point 160 of the sample conduit that is located between vent 157 and immunosensor chip 153.
  • a fluid is introduced into conduit 158.
  • the fluid is present initially within a foil pouch 161 that is punctured by a pin when an actuating means applies pressure upon the pouch.
  • a short conduit 162 that connects the fluid to conduit 154 via a small opening in the gasket 163.
  • a second capillary stop initially prevents the fluid from reaching capillary stop 160, so that the fluid is retained within conduit 158.
  • Air vent 164 preferably comprising a small flap cut in the gasket or a membrane that vibrates to provide an intermittent air stream, provides a means for air to enter conduit 158 via a second vent 165.
  • the second vent 165 preferably also contains wicking material capable of closing the vent if wetted, which permits subsequent depression of sample diaphragm 156 to close vent 165, if required.
  • fluid is drawn from conduit 158, through capillary stop 160, into conduit 154. Because the flow of fluid is interaipted by air entering vent 164, at least one air segment (e.g., a segment or stream of segments) is introduced.
  • sample diaphragm 156 draws the liquid containing at least one air segment back across the sensing surface of sensor chip 153.
  • the presence of air-liquid boundaries within the liquid enhances the rinsing of the sensor chip surface to remove remaining sample.
  • the movement of the sample diaphragm 156 is controlled in conjunction with signals received from the conductivity electrodes housed within the sensor chip adjacent to the analyte sensors. In this way, the presence of liquid over the sensor is detected, and multiple readings can be performed by movement of the fluid in discrete steps.
  • the ground chip 165 is preferably a silver/silver chloride reference electrode and acts effectively as both a counter and reference electrode in an amperometric measurement. It is advantageous, in this embodiment of the invention, to avoid air segments, which easily form upon the relatively hydrophobic silver chloride surface, to pattern the ground chip as small regions of silver/silver chloride interspersed with more hydrophilic regions, such as a surface of silicon dioxide.
  • a preferred ground electrode (counter and reference electrode combined) configuration comprises an array of silver/silver chloride squares densely arranged and interspersed with silicon dioxide. There is a further advantage in the avoidance of unintentional segments if the regions of silver/silver chloride are somewhat recessed.
  • Regions R1-R7 represent specific regions of the conduits associated with specific operational functions.
  • Rl represents the sample chamber;
  • R2 the sample conduit whereby a metered portion of the sample is transferred to the capture region, and in which the sample is optionally amended with a substance coated upon the walls of the conduit (e.g., magnetically susceptible beads with antibody to the analyte and antibody conjugate);
  • R21 represents a region for mixing and sample oscillation;
  • R3 represents the magnetic capture region, which houses the imiminosensors;
  • R4 and R5 represent portions of the first conduit that are optionally used for further amendment of fluids with substances coated onto the conduit wall, whereby more complex assay schemes are achieved;
  • R6 represents the portion of the second conduit into which fluid is introduced upon insertion of the cartridge into a reading apparatus;
  • R7 comprises a portion of the conduit located between capillary stops 160 and 166 (shown in FIG. 35
  • Example 5 Magnetic Immunosensor System
  • This example addresses the coordination of fluidics and analyte measurements as a system.
  • a user places a sample into the cartridge, places the cartridge into the analyzer and in about 1 minute to about 20 minutes, a quantitative measurement of one or more analytes is performed.
  • FIGS. 35 and 36 the following is a non-limiting example of a sequence of events that occur during the analysis:
  • a 25 to 50 ⁇ sample is introduced in the sample inlet 167 and fills chamber 151 to capillary stop 152 formed by a 0.012" laser cut hole in the adhesive tape holding the cover and base components together. The user then seals the inlet and places the cartridge into the analyzer.
  • Magnetically susceptible beads having antibodies to the analyte of interest and optionally a labeled conjugate antibody to the analyte may be provided in a dry coating on the walls of chamber 151 such that they dissolve into the sample once the sample is introduced therein.
  • either or both the magnetically susceptible beads and the labeled conjugate antibody may be provided in a dry coating within conduit 154.
  • the analyzer makes contact with the cartridge, and a motor driven plunger presses onto the foil pouch 161, forcing the wash/analysis fluid out into a central conduit 158.
  • a separate motor driven plunger contacts the sample diaphragm 156, pushing a measured segment of the sample along the sample conduit (from reagent region Rl to R2 and R21).
  • the sample position is detected via one or more conductivity sensors.
  • the immunosensor chip is located in capture region R3.
  • the sample is oscillated by means of the sample diaphragm 156 in the R21 region in a predetermined and controlled fashion for a controlled time to promote binding of analyte to the magnetically susceptible beads and to the antibody conjugate.
  • wash/analysis fluid from the second conduit moves into the inlet located between R5 and R4.
  • a series of air separated fluid segments are generated, which are pulled across the sensor chip towards the sample inlet (from R4 to R3 to R21 to R2 and Rl). This process washes the sensor free of excess reagents and wets the sensor with reagents appropriate for the analysis.
  • the wash/analysis fluid that originates in the foil pouch can be further amended by addition of reagents in R7 and R6 within the central wash/analysis fluid conduit.
  • the wash/analysis fluid segment is drawn at a slower speed towards the sample inlet to yield an immunosensor chip with the retained magnetically susceptible beads, which contains only a thin layer of the analysis fluid.
  • the electrochemical analysis is performed at this point.
  • the preferred method of analysis is amperometry, but potentiometry or impedance detection is also used.

Abstract

La présente invention concerne un appareil et des méthodes de détermination rapide de la présence d'analytes dans des échantillons de liquide, faisant appel à des immunoessais impliquant la capture magnétique de billes sur un capteur pouvant être utilisé dans le cadre du diagnostic sur le lieu de soins.
PCT/US2011/046753 2010-08-05 2011-08-05 Méthode et dispositif d'immunoessai impliquant la capture de billes sensibles au magnétisme WO2012019104A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP11746727.4A EP2600973B1 (fr) 2010-08-05 2011-08-05 Méthode et dispositif d'immunoessai impliquant la capture de billes sensibles au magnétisme
CN201180046212.8A CN103118785B (zh) 2010-08-05 2011-08-05 使用易感磁微珠捕获的免疫测定方法和装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37106610P 2010-08-05 2010-08-05
US61/371,066 2010-08-05

Publications (1)

Publication Number Publication Date
WO2012019104A1 true WO2012019104A1 (fr) 2012-02-09

Family

ID=44504268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/046753 WO2012019104A1 (fr) 2010-08-05 2011-08-05 Méthode et dispositif d'immunoessai impliquant la capture de billes sensibles au magnétisme

Country Status (4)

Country Link
US (2) US10126296B2 (fr)
EP (1) EP2600973B1 (fr)
CN (1) CN103118785B (fr)
WO (1) WO2012019104A1 (fr)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103048452A (zh) * 2012-12-18 2013-04-17 苏州浩欧博生物医药有限公司 一种肿瘤相关抗原ca125的纳米磁微粒化学发光测定试剂盒及其制备方法和检测方法
CN103048454A (zh) * 2012-12-18 2013-04-17 苏州浩欧博生物医药有限公司 一种乙型肝炎病毒e抗原的纳米磁微粒化学发光测定试剂盒及其制备方法和检测方法
CN103048474A (zh) * 2012-12-18 2013-04-17 苏州浩欧博生物医药有限公司 一种促甲状腺激素的纳米磁微粒化学发光测定试剂盒及其制备方法和检测方法
CN105092834A (zh) * 2014-05-05 2015-11-25 江苏泽成生物技术有限公司 糖类抗原19-9（ca19-9）定量测定试剂盒及其制备方法与检测方法
US9549914B2 (en) 2014-04-03 2017-01-24 The Johns Hopkins University Treatment of human cytomegalovirus by modulating Wnt
EP3143386A4 (fr) * 2014-05-12 2017-11-29 Qi, Huan Procédé et système de détection d'analytes
US9958440B2 (en) 2010-08-05 2018-05-01 Abbott Point Of Care Inc. Magnetic immunosensor and method of use
US10048258B2 (en) 2010-08-05 2018-08-14 Abbott Point Of Care Inc. Oscillating immunoassay method and device
CN109239046A (zh) * 2018-08-22 2019-01-18 暨南大学 一种c反应蛋白检测试剂及sers检测方法
CN110192109A (zh) * 2016-12-09 2019-08-30 雅培医护站股份有限公司 用于扩展范围的灵敏度的联合免疫测定和磁免疫测定系统和装置
US11402375B2 (en) 2010-08-05 2022-08-02 Abbott Point Of Care Inc. Magnetic immunosensor with trench configuration and method of use

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201014805D0 (en) 2010-09-07 2010-10-20 Multi Sense Technologies Ltd Microfluidics based assay device
GB201218555D0 (en) * 2012-10-16 2012-11-28 Mode Diagnostics Ltd Immunoassay using electrochemical detection
CN103048477B (zh) * 2012-12-18 2015-07-01 苏州浩欧博生物医药有限公司 一种三碘甲状腺原氨酸的纳米磁微粒化学发光测定试剂盒及其制备方法和检测方法
CN103513028B (zh) * 2013-09-11 2015-08-12 辽宁师范大学 一种基于电位控制固定抗体的免疫传感器制备方法
AU2014322925B2 (en) * 2013-09-17 2020-07-02 Biomerieux Solution for dissociating vitamin D from vitamin D-binding protein, associated detection method and use
GB201317621D0 (en) * 2013-10-04 2013-11-20 Randox Teoranta Kidney disease biomarker
CN104569376B (zh) * 2013-10-09 2017-11-10 常州博闻迪医药科技有限公司 一种磁微粒介导的层析检测技术装置及其用途
CN107076752A (zh) * 2014-07-02 2017-08-18 曼普知识产权控股有限公司 检测异位妊娠的方法
AU2014407088B2 (en) 2014-09-26 2021-09-23 Somalogic Operating Co., Inc. Cardiovascular risk event prediction and uses thereof
CN104237513A (zh) * 2014-09-30 2014-12-24 博奥赛斯（天津）生物科技有限公司 一种甲状腺过氧化物酶抗体磁微粒化学发光免疫定量检测试剂盒
MX2017012742A (es) * 2015-04-03 2017-11-15 Abbott Lab Dispositivos y metodos para el analisis de muestras.
CN104950111A (zh) * 2015-05-22 2015-09-30 北京协和洛克生物技术有限责任公司 一种定量检测样本中髓过氧化物酶浓度的液态芯片试剂盒及其制备方法
JP6738608B2 (ja) * 2016-01-22 2020-08-12 田中貴金属工業株式会社 クロマトグラフ媒体
GB201611442D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Fluid control
ES2849173T3 (es) * 2016-12-09 2021-08-16 Abbott Point Of Care Inc Métodos combinados de inmunoensayo e inmunoensayo magnético para un intervalo extendido de sensibilidad
US11156607B2 (en) * 2016-12-09 2021-10-26 Abbott Point Of Care Inc. Extended range immunoassay devices with immunosensor and magnetic immunosensor
US10324041B2 (en) * 2016-12-21 2019-06-18 Abbott Japan Co., Ltd. Optical imaging system using lateral illumination for digital assays
CN107328931A (zh) * 2017-05-16 2017-11-07 中山大学 一种基于纳米探针和磁性微纳米颗粒的快速连续检测技术
CN108593938A (zh) * 2018-05-31 2018-09-28 吉林大学 表面偶联心肌肌钙蛋白抗原的磁性高分子微球及其在检测血清中心肌肌钙蛋白中的应用
CN114599977A (zh) * 2019-12-30 2022-06-07 深圳迈瑞生物医疗电子股份有限公司 用于检测传染性疾病的试剂盒、方法及免疫分析仪

Citations (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4230685A (en) 1979-02-28 1980-10-28 Northwestern University Method of magnetic separation of cells and the like, and microspheres for use therein
US4554088A (en) 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4628037A (en) 1983-05-12 1986-12-09 Advanced Magnetics, Inc. Binding assays employing magnetic particles
US4945045A (en) 1984-07-06 1990-07-31 Serono Diagnostics Ltd. Electrochemical methods of assay
US4978610A (en) 1984-07-10 1990-12-18 Forrest Gordon C Method of assay employing a magnetic electrode
US5149630A (en) 1984-01-26 1992-09-22 Forrest Gordon C Methods of assay
US5200051A (en) 1988-11-14 1993-04-06 I-Stat Corporation Wholly microfabricated biosensors and process for the manufacture and use thereof
US5587102A (en) 1994-08-25 1996-12-24 Djs&T Limited Partnership Magnetic paint composition and method
US5821399A (en) 1993-07-16 1998-10-13 I-Stat Corporation Automatic test parameters compensation of a real time fluid analysis sensing device
US5843329A (en) 1995-03-17 1998-12-01 Deetz; Dayton J. Magnetic paint or ink additive
US5998224A (en) 1997-05-16 1999-12-07 Abbott Laboratories Magnetically assisted binding assays utilizing a magnetically responsive reagent
US6231760B1 (en) 1995-02-21 2001-05-15 Iqbal W. Siddiqi Apparatus for mixing and separation employing magnetic particles
US6294342B1 (en) 1999-09-29 2001-09-25 Abbott Laboratories Magnetically assisted binding assays utilizing a magnetically responsive reagent
US20030012693A1 (en) * 2000-08-24 2003-01-16 Imego Ab Systems and methods for localizing and analyzing samples on a bio-sensor chip
US6764859B1 (en) 1999-07-19 2004-07-20 Biomerieux, B.V. Device and method for mixing magnetic particles with a fluid
US7106051B2 (en) 2001-12-21 2006-09-12 Koninklijke Philips Electronics, N.V. Magnetoresistive sensing device, system and method for determining a density of magnetic particles in fluid
US7223438B2 (en) 2002-09-17 2007-05-29 Northwestern University Patterning magnetic nanostructures
US20080160634A1 (en) * 2006-12-28 2008-07-03 Xing Su Method and device for biomolecule preparation and detection using magnetic array
US7419821B2 (en) 2002-03-05 2008-09-02 I-Stat Corporation Apparatus and methods for analyte measurement and immunoassay
US20090191401A1 (en) 2005-04-28 2009-07-30 Dayton Joseph Deetz Magnetic paint
US7682833B2 (en) 2003-09-10 2010-03-23 Abbott Point Of Care Inc. Immunoassay device with improved sample closure
US7723099B2 (en) 2003-09-10 2010-05-25 Abbott Point Of Care Inc. Immunoassay device with immuno-reference electrode
US20100248273A1 (en) 2009-03-25 2010-09-30 Abbott Point Of Care Inc. Amelioration of heterophile antibody immunosensor interference
US20110117581A1 (en) 2009-11-17 2011-05-19 Abbott Point Of Care Inc. Reducing leukocyte interference in competitive immunoassays
US20110117580A1 (en) 2009-11-17 2011-05-19 Abbott Point Of Care Inc. Reducing leukocyte interference in non-competitive immunoassays

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4849340A (en) 1987-04-03 1989-07-18 Cardiovascular Diagnostics, Inc. Reaction system element and method for performing prothrombin time assay
ZA92803B (en) 1991-02-06 1992-11-25 Igen Inc Method and apparatus for magnetic microparticulate based luminescene asay including plurality of magnets
US5445971A (en) 1992-03-20 1995-08-29 Abbott Laboratories Magnetically assisted binding assays using magnetically labeled binding members
US5422621A (en) 1993-10-29 1995-06-06 International Business Machines Corporation Oriented granular giant magnetoresistance sensor
US6673533B1 (en) * 1995-03-10 2004-01-06 Meso Scale Technologies, Llc. Multi-array multi-specific electrochemiluminescence testing
DE19822123C2 (de) 1997-11-21 2003-02-06 Meinhard Knoll Verfahren und Vorrichtung zum Nachweis von Analyten
WO2000051814A1 (fr) 1999-03-05 2000-09-08 Bio-Rad Laboratories, Inc. Analyse simultanee d'un analyte et d'un interferant au moyen de la cytometrie de flux
KR100533097B1 (ko) 2000-04-27 2005-12-02 티디케이가부시기가이샤 복합자성재료와 이것을 이용한 자성성형재료, 압분 자성분말성형재료, 자성도료, 복합 유전체재료와 이것을이용한 성형재료, 압분성형 분말재료, 도료, 프리프레그및 기판, 전자부품
AU2001261463A1 (en) 2000-05-12 2001-11-26 University Of Cincinnati Magnetic bead-based arrays
US20060207877A1 (en) 2001-01-30 2006-09-21 Walter Schmidt Microfluidic device with various surface properties fabricated in multilayer body by plasma etching
US20030040129A1 (en) 2001-08-20 2003-02-27 Shah Haresh P. Binding assays using magnetically immobilized arrays
US20040210289A1 (en) 2002-03-04 2004-10-21 Xingwu Wang Novel nanomagnetic particles
US20060134713A1 (en) * 2002-03-21 2006-06-22 Lifescan, Inc. Biosensor apparatus and methods of use
US7572355B1 (en) 2004-01-07 2009-08-11 Board Of Trustees Of The University Of Arkansas Electrochemistry using permanent magnets with electrodes embedded therein
US20060257854A1 (en) 2004-02-27 2006-11-16 Mcdevitt John T Membrane assay system including preloaded particles
US20060292700A1 (en) 2005-06-22 2006-12-28 Naishu Wang Diffused interrupted lateral flow immunoassay device and method
EP1814172A1 (fr) 2006-01-27 2007-08-01 IEE International Electronics & Engineering S.A.R.L. Elément de détection d'un champ magnétique
DE102006029032A1 (de) 2006-02-17 2007-08-23 Poly-An Gesellschaft zur Herstellung von Polymeren für spezielle Anwendungen und Analytik mbH Vorrichtung, Verfahren und Kit zum Nachweis von Analyten in einer Probe
EP2007905B1 (fr) * 2006-03-15 2012-08-22 Micronics, Inc. Dosages d'acides nucléiques intégrés
GB2436616A (en) * 2006-03-29 2007-10-03 Inverness Medical Switzerland Assay device and method
US8870446B2 (en) 2006-06-21 2014-10-28 Spinomix S.A. Device and method for manipulating and mixing magnetic particles in a liquid medium
WO2008094198A2 (fr) * 2006-07-28 2008-08-07 Biosite Incorporated Dispositifs et procédés pour effectuer des tests de liaison à des récepteurs en utilisant des particules magnétiques
EP1939629A3 (fr) 2006-08-11 2011-03-09 Samsung Electronics Co., Ltd. Force centrifuge basée selon un dispositif de contrôle de position magnétique et système micro fluide sous forme de disque
JP5221549B2 (ja) * 2006-10-12 2013-06-26 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 試薬層を有した高速バイオセンサ
US20100015728A1 (en) * 2006-12-19 2010-01-21 Iverness Medical Switzerland Gmbh Assay Device and Method
WO2008099346A1 (fr) 2007-02-16 2008-08-21 Koninklijke Philips Electronics N. V. Procédé et système de séparation destinés à séparer des particules magnétiques, colonne de séparation à utiliser dans un système de séparation
WO2008121239A1 (fr) * 2007-03-29 2008-10-09 Response Biomedical Corporation Système et appareil de lecteur de test modulaire
JP4772816B2 (ja) 2008-03-21 2011-09-14 株式会社東芝 マイクロアレイおよびネガティブコントロールプローブの設計方法
JP5744906B2 (ja) 2009-12-18 2015-07-08 アボット ポイント オブ ケア インコーポレイテッド 試料分析用の一体型ヒンジ式カートリッジ・ハウジング
CA2787135C (fr) 2010-01-21 2018-11-20 Biocep Ltd. Separation magnetique de cellules rares
US8394325B2 (en) 2010-06-14 2013-03-12 Abbott Point Of Care Inc. Magnetic beads for reducing leukocyte interference in immunoassays
US11402375B2 (en) * 2010-08-05 2022-08-02 Abbott Point Of Care Inc. Magnetic immunosensor with trench configuration and method of use
CN103154739B (zh) * 2010-08-05 2016-01-06 雅培医护站股份有限公司 磁免疫传感器和使用方法
WO2012019109A1 (fr) * 2010-08-05 2012-02-09 Abbott Point Of Care Inc. Procédé et dispositif d'immuno-essai oscillant
EP2650676A4 (fr) * 2010-12-07 2018-01-17 Hitachi High-Technologies Corporation Électrode pour mesure électrochimique, cellule d'électrolyse pour mesure électrochimique, analyseur pour dosage électrochimique, et procédé de production associé

Patent Citations (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4230685A (en) 1979-02-28 1980-10-28 Northwestern University Method of magnetic separation of cells and the like, and microspheres for use therein
US4554088A (en) 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4628037A (en) 1983-05-12 1986-12-09 Advanced Magnetics, Inc. Binding assays employing magnetic particles
US5149630A (en) 1984-01-26 1992-09-22 Forrest Gordon C Methods of assay
US4945045A (en) 1984-07-06 1990-07-31 Serono Diagnostics Ltd. Electrochemical methods of assay
US4978610A (en) 1984-07-10 1990-12-18 Forrest Gordon C Method of assay employing a magnetic electrode
US5200051A (en) 1988-11-14 1993-04-06 I-Stat Corporation Wholly microfabricated biosensors and process for the manufacture and use thereof
US5554339A (en) 1988-11-14 1996-09-10 I-Stat Corporation Process for the manufacture of wholly microfabricated biosensors
US5821399A (en) 1993-07-16 1998-10-13 I-Stat Corporation Automatic test parameters compensation of a real time fluid analysis sensing device
US5587102A (en) 1994-08-25 1996-12-24 Djs&T Limited Partnership Magnetic paint composition and method
US6231760B1 (en) 1995-02-21 2001-05-15 Iqbal W. Siddiqi Apparatus for mixing and separation employing magnetic particles
US5843329A (en) 1995-03-17 1998-12-01 Deetz; Dayton J. Magnetic paint or ink additive
US5998224A (en) 1997-05-16 1999-12-07 Abbott Laboratories Magnetically assisted binding assays utilizing a magnetically responsive reagent
US6764859B1 (en) 1999-07-19 2004-07-20 Biomerieux, B.V. Device and method for mixing magnetic particles with a fluid
US6294342B1 (en) 1999-09-29 2001-09-25 Abbott Laboratories Magnetically assisted binding assays utilizing a magnetically responsive reagent
US20030012693A1 (en) * 2000-08-24 2003-01-16 Imego Ab Systems and methods for localizing and analyzing samples on a bio-sensor chip
US7106051B2 (en) 2001-12-21 2006-09-12 Koninklijke Philips Electronics, N.V. Magnetoresistive sensing device, system and method for determining a density of magnetic particles in fluid
US7419821B2 (en) 2002-03-05 2008-09-02 I-Stat Corporation Apparatus and methods for analyte measurement and immunoassay
US7223438B2 (en) 2002-09-17 2007-05-29 Northwestern University Patterning magnetic nanostructures
US7682833B2 (en) 2003-09-10 2010-03-23 Abbott Point Of Care Inc. Immunoassay device with improved sample closure
US7723099B2 (en) 2003-09-10 2010-05-25 Abbott Point Of Care Inc. Immunoassay device with immuno-reference electrode
US20090191401A1 (en) 2005-04-28 2009-07-30 Dayton Joseph Deetz Magnetic paint
US20080160634A1 (en) * 2006-12-28 2008-07-03 Xing Su Method and device for biomolecule preparation and detection using magnetic array
US20100248273A1 (en) 2009-03-25 2010-09-30 Abbott Point Of Care Inc. Amelioration of heterophile antibody immunosensor interference
US20110117581A1 (en) 2009-11-17 2011-05-19 Abbott Point Of Care Inc. Reducing leukocyte interference in competitive immunoassays
US20110117580A1 (en) 2009-11-17 2011-05-19 Abbott Point Of Care Inc. Reducing leukocyte interference in non-competitive immunoassays

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BRULS ET AL., LAB CHIP, vol. 9, 2009, pages 3504 - 3510
DITTMER ET AL., CLIN. CHIM. ACTA, 2010
LAURELL ET AL.: "Methods in Enzymology", vol. 73, 1981, ACADEMIC PRESS, article "Electroimmunoassay", pages: 339,340, - 348
M. J. GREEN, PHILOS. TRANS. R. SOC. LOND. B. BIOL. SCI., vol. 316, 1987, pages 135 - 142
PENG Z-C ET AL: "A bead-based electrochemical biosensor with integrated magnetic manipulation for controllable sample preconcentration", SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS CONFERENCE, 2009. TRANSDUCERS 2009. INTERNATIONAL, IEEE, PISCATAWAY, NJ, USA, no. 15TH, 21 June 2009 (2009-06-21), pages 1802 - 1805, XP002659725, ISBN: 978-1-4244-4190-7 *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9958440B2 (en) 2010-08-05 2018-05-01 Abbott Point Of Care Inc. Magnetic immunosensor and method of use
US11402375B2 (en) 2010-08-05 2022-08-02 Abbott Point Of Care Inc. Magnetic immunosensor with trench configuration and method of use
US10145843B2 (en) 2010-08-05 2018-12-04 Abbott Point Of Care Inc. Magnetic immunosensor and method of use
US10048258B2 (en) 2010-08-05 2018-08-14 Abbott Point Of Care Inc. Oscillating immunoassay method and device
CN103048474B (zh) * 2012-12-18 2015-07-01 苏州浩欧博生物医药有限公司 一种促甲状腺激素的纳米磁微粒化学发光测定试剂盒及其制备方法和检测方法
CN103048452A (zh) * 2012-12-18 2013-04-17 苏州浩欧博生物医药有限公司 一种肿瘤相关抗原ca125的纳米磁微粒化学发光测定试剂盒及其制备方法和检测方法
CN103048452B (zh) * 2012-12-18 2015-04-15 苏州浩欧博生物医药有限公司 一种肿瘤相关抗原ca125的纳米磁微粒化学发光测定试剂盒及其制备方法和检测方法
CN103048474A (zh) * 2012-12-18 2013-04-17 苏州浩欧博生物医药有限公司 一种促甲状腺激素的纳米磁微粒化学发光测定试剂盒及其制备方法和检测方法
CN103048454A (zh) * 2012-12-18 2013-04-17 苏州浩欧博生物医药有限公司 一种乙型肝炎病毒e抗原的纳米磁微粒化学发光测定试剂盒及其制备方法和检测方法
US9549914B2 (en) 2014-04-03 2017-01-24 The Johns Hopkins University Treatment of human cytomegalovirus by modulating Wnt
CN105092834A (zh) * 2014-05-05 2015-11-25 江苏泽成生物技术有限公司 糖类抗原19-9（ca19-9）定量测定试剂盒及其制备方法与检测方法
EP3143386A4 (fr) * 2014-05-12 2017-11-29 Qi, Huan Procédé et système de détection d'analytes
CN110192109A (zh) * 2016-12-09 2019-08-30 雅培医护站股份有限公司 用于扩展范围的灵敏度的联合免疫测定和磁免疫测定系统和装置
US11680944B2 (en) 2016-12-09 2023-06-20 Abbott Point Of Care Inc. Combined immunoassay and magnetic immunoassay systems and devices for extended range of sensitivity
CN110192109B (zh) * 2016-12-09 2023-07-28 雅培医护站股份有限公司 用于扩展范围的灵敏度的联合免疫测定和磁免疫测定系统和装置
CN109239046A (zh) * 2018-08-22 2019-01-18 暨南大学 一种c反应蛋白检测试剂及sers检测方法
CN109239046B (zh) * 2018-08-22 2021-06-11 暨南大学 一种c反应蛋白检测试剂及sers检测方法

Also Published As

Publication number Publication date
CN103118785A (zh) 2013-05-22
EP2600973B1 (fr) 2016-05-25
EP2600973A1 (fr) 2013-06-12
CN103118785B (zh) 2015-11-25
US20190033305A1 (en) 2019-01-31
US10126296B2 (en) 2018-11-13
US20120031773A1 (en) 2012-02-09

Similar Documents

Publication Publication Date Title
US10145843B2 (en) Magnetic immunosensor and method of use
US20220326232A1 (en) Magnetic immunosensor with trench configuration and method of use
US20190033305A1 (en) Immunoassay method and device with magnetically susceptible bead capture
US10048258B2 (en) Oscillating immunoassay method and device
US10514379B2 (en) Dual range cardiac troponin immunoassay devices and methods using immunosensor and magnetic immunosensor
US11680944B2 (en) Combined immunoassay and magnetic immunoassay systems and devices for extended range of sensitivity
US20210156852A1 (en) Combined immunoassay and magnetic immunoassay methods for extended range of sensitivity
US20220034874A1 (en) Extended range immunoassay devices with immunosensor and magnetic immunosensor
US10935511B2 (en) Ameliorated crosstalk immunoassay test device for determining a concentration of an analyte
US10871488B2 (en) Crossover analytical systems and methods using an immunosensor and magnetic immunosensor

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 201180046212.8

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11746727

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2011746727

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: DE