WO2012008768A3 - Method for preparing transformed potato plants having multispecies virus resistance, and potato plants prepared by same - Google Patents

Method for preparing transformed potato plants having multispecies virus resistance, and potato plants prepared by same Download PDF

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Publication number
WO2012008768A3
WO2012008768A3 PCT/KR2011/005180 KR2011005180W WO2012008768A3 WO 2012008768 A3 WO2012008768 A3 WO 2012008768A3 KR 2011005180 W KR2011005180 W KR 2011005180W WO 2012008768 A3 WO2012008768 A3 WO 2012008768A3
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WO
WIPO (PCT)
Prior art keywords
potato
potato plants
virus
prepared
multispecies
Prior art date
Application number
PCT/KR2011/005180
Other languages
French (fr)
Korean (ko)
Other versions
WO2012008768A2 (en
Inventor
정봉남
최국선
피터 팔루카이티스
Original Assignee
대한민국(농촌진흥청장)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 대한민국(농촌진흥청장) filed Critical 대한민국(농촌진흥청장)
Priority to KR1020127015778A priority Critical patent/KR101457958B1/en
Publication of WO2012008768A2 publication Critical patent/WO2012008768A2/en
Publication of WO2012008768A3 publication Critical patent/WO2012008768A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

The present invention relates to a method for preparing transformed potato plants, according to which potato cells are transformed with a recombinant expression vector containing a fusion gene (YLRATRPM) or a fusion gene (YLRA), prepared by fusing genetic fragments obtained from each of the following five plant viruses: potato virus Y (PVY), potato leafroll virus (PLRV), potato virus A(PVA), tobacco rattle virus (TRV) and potato mop-top virus (PMTV), thus enabling the potato plants to exhibit resistance to all of the five or to all of three viruses used in the preparation of the fusion gene. The present invention also relates to the transformed potato plants prepared by the method.
PCT/KR2011/005180 2010-07-14 2011-07-14 Method for preparing transformed potato plants having multispecies virus resistance, and potato plants prepared by same WO2012008768A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020127015778A KR101457958B1 (en) 2010-07-14 2011-07-14 Method for preparing transformed potato plants having multispecies virus resistance, and potato plants prepared by same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100067991 2010-07-14
KR10-2010-0067991 2010-07-14

Publications (2)

Publication Number Publication Date
WO2012008768A2 WO2012008768A2 (en) 2012-01-19
WO2012008768A3 true WO2012008768A3 (en) 2012-05-18

Family

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Family Applications (1)

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PCT/KR2011/005180 WO2012008768A2 (en) 2010-07-14 2011-07-14 Method for preparing transformed potato plants having multispecies virus resistance, and potato plants prepared by same

Country Status (2)

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KR (1) KR101457958B1 (en)
WO (1) WO2012008768A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110857439B (en) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 Potato Y virus gene segment capable of efficiently generating siRNA, attenuated vaccine, preparation method and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010112952A (en) * 1999-05-10 2001-12-22 릴리 엠 씨즐러 스피허, 아네뜨 워너 Regulation of viral gene expression

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100358755B1 (en) * 2000-03-04 2002-11-07 대한민국 Transgenic tomato resistant to virus and process for preparation thereof
KR100846174B1 (en) * 2006-12-19 2008-07-16 대한민국 4 Multiplex detection primer set for PVY PLRV PVX and PVS infecting potato and the method diagnosing infectious diseases by using the same

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010112952A (en) * 1999-05-10 2001-12-22 릴리 엠 씨즐러 스피허, 아네뜨 워너 Regulation of viral gene expression

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHANG-XIANG ZHU ET AL.: "Induction of RNA-mediated Multiple Virus Resistance to Potato virus Y, Tobacco mosaic virus and Cucumber mosaic virus", J. PHYTOPATHOLOGY, vol. 157, 2009, pages 101 - 107 *
CHUNG ET AL.: "Resistance to multiple viruses in transgenic tobacco expressing fused, tandem repeat, virus-derived double-stranded RNAs", VIRUS GENES, vol. 43, no. 3, 19 August 2011 (2011-08-19), pages 454 - 464, XP019955342, DOI: doi:10.1007/s11262-011-0655-z *
JENNIFER K. LODGE ET AL.: "Broad-spectrum virus resistance in transgenic plants expressing pokeweed antiviral protein", PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 7089 - 7093, XP002923376, DOI: doi:10.1073/pnas.90.15.7089 *

Also Published As

Publication number Publication date
KR20120115498A (en) 2012-10-18
KR101457958B1 (en) 2014-11-04
WO2012008768A2 (en) 2012-01-19

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