WO2012007919A3 - Construction d'acide nucléique destinée à accroître la tolérance au stress abiotique dans des végétaux - Google Patents

Construction d'acide nucléique destinée à accroître la tolérance au stress abiotique dans des végétaux Download PDF

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Publication number
WO2012007919A3
WO2012007919A3 PCT/IB2011/053150 IB2011053150W WO2012007919A3 WO 2012007919 A3 WO2012007919 A3 WO 2012007919A3 IB 2011053150 W IB2011053150 W IB 2011053150W WO 2012007919 A3 WO2012007919 A3 WO 2012007919A3
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Prior art keywords
nucleic acid
plants
stress
abiotic stress
stress tolerance
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PCT/IB2011/053150
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English (en)
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WO2012007919A2 (fr
WO2012007919A9 (fr
Inventor
Shimon Gepstein
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Technion Research & Development Foundation Ltd.
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Application filed by Technion Research & Development Foundation Ltd. filed Critical Technion Research & Development Foundation Ltd.
Priority to EP11749521.8A priority Critical patent/EP2593554A2/fr
Priority to CA2804728A priority patent/CA2804728A1/fr
Priority to BR112013000984A priority patent/BR112013000984A2/pt
Priority to CN201180044363.XA priority patent/CN103108955B/zh
Priority to US13/810,246 priority patent/US10106812B2/en
Publication of WO2012007919A2 publication Critical patent/WO2012007919A2/fr
Publication of WO2012007919A3 publication Critical patent/WO2012007919A3/fr
Publication of WO2012007919A9 publication Critical patent/WO2012007919A9/fr

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8295—Cytokinins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne des constructions d'acide nucléique qui comprennent un polynucléotide codant pour le phosphate d'adénosine-isopentényle transférase (IPT) lié fonctionnellement à un promoteur lié au stress. L'invention porte en outre sur des cellules hôtes transformées avec lesdites constructions d'acide nucléique, et sur des plantes transgéniques exprimant la séquence codante de l'IPT en cours de régulation transcriptionnelle d'un promoteur lié au stress. L'invention a également trait à leurs procédés d'utilisation, en vue d'accroître la tolérance au stress abiotique d'un végétal et la biomasse de végétaux dans des conditions normales ou de stress.
PCT/IB2011/053150 2010-07-15 2011-07-14 Construction d'acide nucléique destinée à accroître la tolérance au stress abiotique dans des végétaux WO2012007919A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP11749521.8A EP2593554A2 (fr) 2010-07-15 2011-07-14 Construction d'acide nucléique destinée à accroître la tolérance au stress abiotique dans des végétaux
CA2804728A CA2804728A1 (fr) 2010-07-15 2011-07-14 Construction d'acide nucleique destinee a accroitre la tolerance au stress abiotique dans des vegetaux
BR112013000984A BR112013000984A2 (pt) 2010-07-15 2011-07-14 Estrutura de ácido nucleico para o aumento da tolerância ao estresse abiótico em plantas
CN201180044363.XA CN103108955B (zh) 2010-07-15 2011-07-14 用于提高植物中非生物胁迫耐受性的核酸构建体
US13/810,246 US10106812B2 (en) 2010-07-15 2011-07-14 Nucleic acid construct for increasing abiotic stress tolerance in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36440710P 2010-07-15 2010-07-15
US61/364,407 2010-07-15

Publications (3)

Publication Number Publication Date
WO2012007919A2 WO2012007919A2 (fr) 2012-01-19
WO2012007919A3 true WO2012007919A3 (fr) 2012-04-12
WO2012007919A9 WO2012007919A9 (fr) 2012-09-27

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Country Status (6)

Country Link
US (1) US10106812B2 (fr)
EP (1) EP2593554A2 (fr)
CN (1) CN103108955B (fr)
BR (1) BR112013000984A2 (fr)
CA (1) CA2804728A1 (fr)
WO (1) WO2012007919A2 (fr)

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WO2012007919A2 (fr) 2010-07-15 2012-01-19 Technion Research & Development Foundation Ltd. Construction d'acide nucléique destinée à accroître la tolérance au stress abiotique dans des végétaux
GB201106845D0 (en) * 2011-04-26 2011-06-01 Vib Vzw Methods and means to produce abiotic stress tolerant plants
CA2863139A1 (fr) * 2012-03-02 2013-09-06 Basf Plant Science Company Gmbh Cassettes d'expression pour l'expression d'un gene induite par le stress chez des plantes
CN104884619B (zh) * 2012-12-31 2017-09-08 创世纪种业有限公司 一种棉花异戊烯基转移酶ipt2及其编码基因与应用
CA2904234A1 (fr) * 2013-03-05 2014-09-12 The Texas A&M University System Manipulation de genes phb pour l'accumulation et le rendement de biomasse vegetale
WO2016092570A2 (fr) * 2014-12-10 2016-06-16 Council Of Scientific & Industrial Research Nouveau promoteur bidirectionnel spécifique des sacs embryonnaires d'arabidopsis
WO2016175132A1 (fr) * 2015-04-30 2016-11-03 国立大学法人 奈良先端科学技術大学院大学 Molécule d'adn codant une 5'utr qui permet un niveau élevé d'expression d'une protéine recombinante dans une plante
CN107258356A (zh) * 2017-06-21 2017-10-20 四川农业大学 利用 iaa缓解叶用莴苣镉胁迫生长抑制并降低镉含量的方法
CN109053870B (zh) * 2018-08-08 2021-11-12 中国科学院植物研究所 AtERF49基因在植物响应高温胁迫过程中的应用
CN112226441B (zh) * 2019-06-27 2022-09-30 中国科学技术大学 一种与植物抗逆性相关基因及其编码蛋白与应用
WO2022067019A1 (fr) * 2020-09-26 2022-03-31 The Regents Of The University Of California Protocoles hybrides et schémas de codage à barres pour technologies de séquençage multiples
US20240132908A1 (en) * 2020-12-03 2024-04-25 Inari Agriculture Technology, Inc. Pest and pathogen resistant soybean plants
CN113373161B (zh) * 2021-08-03 2022-07-08 中国农业科学院棉花研究所 GhERF017基因在调控植物耐盐性中的应用
WO2023183820A2 (fr) * 2022-03-21 2023-09-28 City Of Hope Agents thérapeutiques pour traiter une infection des voies respiratoires dirigés contre la covid-19
CN116042646A (zh) * 2022-10-17 2023-05-02 惠州学院 AtLecRK-VI.1基因及其编码蛋白在调控植物根长中的应用
CN116574713A (zh) * 2022-10-18 2023-08-11 扬州大学 Rsb11优异等位变异rsb11-r及其在改良水稻纹枯病抗性中的应用
CN116508439A (zh) * 2023-04-17 2023-08-01 西北工业大学深圳研究院 一种在淹涝胁迫下促进作物种子萌发的方法

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CA2804728A1 (fr) 2012-01-19
WO2012007919A2 (fr) 2012-01-19
BR112013000984A2 (pt) 2017-10-03
EP2593554A2 (fr) 2013-05-22
CN103108955A (zh) 2013-05-15
CN103108955B (zh) 2015-11-25
WO2012007919A9 (fr) 2012-09-27
US10106812B2 (en) 2018-10-23

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