WO2011148512A1 - Procédé de réduction du chrome hexavalent, procédé de production d'un corps moulé et procédé d'amélioration des sols - Google Patents

Procédé de réduction du chrome hexavalent, procédé de production d'un corps moulé et procédé d'amélioration des sols Download PDF

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WO2011148512A1
WO2011148512A1 PCT/JP2010/059156 JP2010059156W WO2011148512A1 WO 2011148512 A1 WO2011148512 A1 WO 2011148512A1 JP 2010059156 W JP2010059156 W JP 2010059156W WO 2011148512 A1 WO2011148512 A1 WO 2011148512A1
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hexavalent chromium
cellulomonas
reducing
cement
microorganism
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PCT/JP2010/059156
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English (en)
Japanese (ja)
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浩 亦野
健次 山本
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株式会社 メニコン
株式会社 大栄工業
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Priority to JP2012517078A priority Critical patent/JP5602848B2/ja
Priority to PCT/JP2010/059156 priority patent/WO2011148512A1/fr
Publication of WO2011148512A1 publication Critical patent/WO2011148512A1/fr

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    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B28/00—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements
    • C04B28/02—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements containing hydraulic cements other than calcium sulfates
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20—Organic substances
    • A62D2101/24—Organic substances containing heavy metals
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/40—Inorganic substances
    • A62D2101/43—Inorganic substances containing heavy metals, in the bonded or free state
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2103/00—Function or property of ingredients for mortars, concrete or artificial stone
    • C04B2103/0068—Ingredients with a function or property not provided for elsewhere in C04B2103/00
    • C04B2103/0096—Reducing agents
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2111/00—Mortars, concrete or artificial stone or mixtures to prepare them, characterised by specific function, property or use
    • C04B2111/10—Compositions or ingredients thereof characterised by the absence or the very low content of a specific material
    • C04B2111/1075—Chromium-free or very low chromium-content materials
    • C04B2111/1081—Chromium VI, e.g. for avoiding chromium eczema
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Definitions

  • the present invention relates to a hexavalent chromium reducing method for reducing hexavalent chromium in a hydraulic substance whose aqueous solution is alkaline, and a method for producing a molded body and a ground improvement method using the hexavalent chromium reducing method. .
  • cement using a hydraulic substance such as cement is widely used as a building material or building material.
  • cement since cement uses natural resources as raw materials, it contains a small amount of hexavalent chromium derived from nature. Therefore, conventionally, techniques for reducing toxic hexavalent chromium to nontoxic trivalent chromium and suppressing elution of hexavalent chromium from concrete have been studied.
  • Patent Document 1 describes a method for suppressing environmental pollution caused by elution of hexavalent chromium from concrete. Specifically, Patent Document 1 discloses a method for preventing elution of hexavalent chromium from the surface of a concrete member by attaching a wood carbide to the surface of the concrete member in a layered or film form.
  • Patent Document 1 in order to completely prevent the elution of hexavalent chromium, it is necessary to cover the entire surface of the concrete with wood carbide, and there is a problem that it cannot be easily carried out if it is buried in the ground or if the object is large. .
  • Patent Documents 2 and 3 describe bioremediation techniques using microorganisms that reduce hexavalent chromium.
  • Patent Document 2 discloses Bacillus pumilus having the ability to reduce hexavalent chromium.
  • Patent Document 3 discloses Actinomycetales having the ability to reduce hexavalent chromium.
  • the hydraulic materials particularly cement has a problem that many microorganisms including those disclosed in Patent Documents 2 and 3 cannot grow because the aqueous solution is a strong alkali, for example, pH 12-13.
  • the concrete is neutralized by the acid derived from the microorganisms and the concrete is deteriorated. For this reason, a method for suppressing elution of hexavalent chromium from concrete using microorganisms has been hardly studied.
  • An object of the present invention is to provide a method for reducing hexavalent chromium and a method for producing a molded body and a ground improvement method using the method for reducing hexavalent chromium.
  • a method for reducing hexavalent chromium which comprises contacting a alkalophilic Cellulomonas microorganism with a hydraulic substance containing hexavalent chromium and having an alkaline aqueous solution.
  • the reducing sugar is preferably at least one selected from xylose, lactose, and maltose.
  • the reducing agent is preferably at least one selected from ascorbic acid, thioglycolic acid, cysteine, mercapto compound, sulfite, bisulfite, and thiosulfate.
  • the hydraulic substance is preferably cement.
  • the cement is preferably at least one selected from Portland cement, mixed cement, and cement-based solidifying material for ground improvement.
  • the alkalophilic Cerulomonas genus microorganism was added to Cellulomonas genus microorganism K32A strain (patent biological deposit center of the National Institute of Advanced Industrial Science and Technology, which is an international depositary organization) (formerly Institute of Biotechnology, Institute of Industrial Science and Technology, Ministry of International Trade and Industry). It has been deposited internationally on May 25, and the bacteria accession number is preferably FERM BP-6766).
  • a hydraulic substance, aggregate, admixture, alkalophilic Cellulomonas genus microorganism, and water containing hexavalent chromium and having an aqueous solution alkaline is provided.
  • a soil-improving cement-based solidifying material containing hexavalent chromium and having an alkaline aqueous solution and an alkaliphilic Cellulomonas microorganism are added to soil.
  • -A ground improvement method to be mixed is provided.
  • the hexavalent chromium reducing method of the present embodiment is carried out by bringing a alkalophilic Cellulomonas microorganism into contact with a hydraulic substance containing hexavalent chromium and having an alkaline aqueous solution (hereinafter simply referred to as “hydraulic substance”). Is done.
  • the above reduction method preferably further includes the addition of reducing sugar, reducing agent, and blast furnace slag.
  • the hydraulic substance is a processing object to be processed by the hexavalent chromium reduction method of the present embodiment.
  • the hydraulic substance include cement, lime, calcium silicate, calcium carbonate, magnesium carbonate, and magnesium trisilicate.
  • the cement include Portland cement, mixed cement, special cement, and cement-based solidifying material for ground improvement.
  • Portland cement include ordinary Portland cement, early-strength Portland cement, ultra-early strong Portland cement, moderately hot Portland cement, sulfate-resistant Portland cement, and low heat Portland cement.
  • Examples of the mixed cement include blast furnace cement, fly ash cement, and silica cement.
  • blast furnace cement include Portland cement mixed with a modifying material such as quartzite, diatomaceous earth, and blast furnace slag.
  • An example of the special cement is alumina cement.
  • dust-proof cement can be mentioned.
  • the dust-proof cement include a fluororesin-treated dust-proof cement in which fine fiberized fluororesin, for example, tetrafluoroethylene is uniformly dispersed in the cement.
  • the shape of the hydraulic substance is not particularly limited, but a hydraulic substance powder, a hydraulic substance granule, and a hydraulic substance granule are preferable in order to improve the contact property with an alkaliphilic Cellulomonas genus microorganism.
  • Alkaline-philic Cellulomonas microorganisms grow in the presence of hydraulic substances and reduce hexavalent chromium in hydraulic substances to non-toxic trivalent chromium.
  • the alkalophilic Cellulomonas microorganism is not particularly limited as long as it is a microorganism that can grow in an alkaline environment, but a microorganism that can grow in an environment of preferably pH 10.5 to 13.5, more preferably pH 12 to 13 is selected. . It is preferable that the alkalophilic Cellulomonas microorganism does not produce an acid, for example, an organic acid during the growth process.
  • the alkalophilic Cellulomonas microorganism preferably has heat resistance. Specifically, a microorganism that can grow in an environment of preferably 30 to 55 ° C., more preferably 35 to 50 ° C. is selected. This is because a hydraulic substance such as cement generates heat when it is mixed with water and hardened.
  • the alkaliphilic cellulomonas microorganism it is preferable to use a microorganism capable of producing ⁇ -1,3 glucan.
  • ⁇ -1,3 glucan include curdlan and paramylon.
  • the curdlan is solidified by heating in the presence of moisture to form a gel.
  • the ⁇ -1,3 glucan produced by the alkalophilic cellulomonas microorganisms can prevent cracking of a molded article after the hydraulic substance is solidified, for example, concrete.
  • Cellulomonas sp. K32A strain As an alkalophilic Cellulomonas microorganism, preferably the Cellulomonas sp. K32A strain (Cellulomonas sp. K32A) (International Depositary Agency, National Institute of Advanced Industrial Science and Technology, Patent Biological Depositary Center (formerly the Ministry of International Trade and Industry, Biotechnology Industrial Technology) Research Institute) has been deposited internationally on June 25, 1999, and its bacterial accession number is FERM BP-6766). Cellulomonas sp. Strain K32A can grow even in a strong alkaline environment and does not produce acid during the growth process. The Cellulomonas genus microorganism K32A is a microorganism that can grow in an environment of 30 to 55 ° C.
  • the mixing ratio of the content of the alkalophilic Cellulomonas microorganism to the content of the hydraulic substance during the reduction treatment of hexavalent chromium is appropriately set according to, for example, the type of hydraulic substance, the type of microorganism, and the processing environment .
  • Reducing sugar promotes the reducing action of hexavalent chromium by alkaliphilic cellulomonas microorganisms.
  • reducing sugars include free monosaccharides, reducing disaccharides, and reducing oligosaccharides.
  • free monosaccharides include triose, tetrose, pentose, hexose, and heptose.
  • pentose include ribose, xylose, and arabinose.
  • Hexose includes, for example, glucose, galactose, mannose, and fructose.
  • reducing disaccharides include homobiose and heterobiose.
  • homobiose examples include maltose, cellobiose, isomaltose, and gentiobiose.
  • heterobiose examples include melibiose, lactose, maltulose, and lactulose.
  • reducing oligosaccharides include maltotriose, which is an ⁇ -amylase degradation product of starch and glycogen.
  • reducing sugars xylose, maltose, and lactose, which have a high function of promoting the reducing action of hexavalent chromium by an alkalophilic Cellulomonas microorganism, are preferable.
  • the reducing agent promotes the reducing action of hexavalent chromium by the alkalophilic Cellulomonas microorganisms.
  • the reducing agent include ascorbic acid, thioglycolic acid, cysteine, mercapto compound, sulfite, bisulfite, and thiosulfate.
  • Ascorbic acid for example, ascorbic acid, erythorbic acid, ascorbate, for example sodium ascorbate and potassium ascorbate, erythorbate, for example sodium erythorbate and potassium erythorbate, and derivatives thereof, for example, ascorbic acid sulfate Examples include ester salts.
  • Examples of thioglycolic acid include thioglycolic acid, thioglycolates such as ammonium thioglycolate and sodium thioglycolate, and esters of thioglycolic acid such as glycerin thioglycolate.
  • Examples of cysteine include cysteine, cysteine hydrochloride, and N-acetyl-L-cysteine.
  • mercapto compounds include thioglycerol, thiolactic acid, thiomalic acid, and cysteamine.
  • Examples of sulfites include sulfite, ammonium sulfite, and sodium sulfite.
  • Examples of the bisulfite include ammonium bisulfite and sodium bisulfite.
  • Examples of the thiosulfate include thiosulfuric acid and sodium thiosulfate.
  • Blast furnace slag promotes the reducing action of hexavalent chromium by alkaliphilic Cellulomonas microorganisms.
  • blast furnace slag include iron chloride and ferrous sulfate.
  • the mixing ratio of the content of reducing sugar, reducing agent, or blast furnace slag with respect to the content of hydraulic substance during the reduction treatment of hexavalent chromium is appropriately set according to, for example, the type of the compound and the processing environment.
  • the hexavalent chromium reducing method of the present embodiment is preferably used when a hydraulic substance and a material containing water or moisture are mixed and cured.
  • the above reduction method is used, for example, when a molded body is manufactured by mixing hydraulic substance, aggregate, admixture, and water.
  • the method for adding the alkalophilic Cellulomonas microorganism is not particularly limited, and it can be added until the hydraulic substance and water are mixed and cured.
  • the alkalophilic Cellulomonas microorganism is preferably added when mixing the hydraulic substance and water.
  • Examples of the molded body include concrete, mortar, and cement paste.
  • portland cement and mixed cement are preferably used as the hydraulic substance.
  • Examples of the aggregate include coarse aggregate and fine aggregate. Known coarse aggregates and fine aggregates can be used.
  • a mortar composition can be prepared by using fine aggregates as the aggregate.
  • a concrete composition can be prepared by using fine aggregate and coarse aggregate as the aggregate.
  • Admixtures are materials other than hydraulic substances, water, and aggregates.
  • the purpose is to improve workability (ease of placing work), improve strength and durability, and adjust the setting speed.
  • the admixture is classified into admixtures that do not need to be included in the volume of the cured molded body and admixtures that need to be included in the volume of the cured molded body.
  • An example of an admixture that changes the viscosity of the hydraulic substance at the time of placing is a surfactant.
  • the admixture for improving workability include an AE agent, a water reducing agent, a fluidizing agent, an AE water reducing agent, a separation reducing agent, and a high performance AE water reducing agent.
  • Examples of the admixture for adjusting the setting speed include accelerators, retarders, early strengthening agents, and quick setting agents.
  • Examples of the admixture for improving strength, heat resistance, and heat insulation include a water reducing agent, a foaming agent, and an expanding agent.
  • Examples of the admixture for improving durability include an AE agent, a rust preventive agent, and a waterproofing agent.
  • As a mixing method of the hydraulic substance and water a known method can be appropriately employed.
  • the hexavalent chromium reducing method of the present embodiment can be used when the ground is improved by adding and mixing the cement-based solidifying material for ground improvement to the soil.
  • the alkaliphilic Cellulomonas genus microorganism is added when the cement-based solidifying material for soil improvement and the soil are mixed.
  • the hexavalent chromium in the hydraulic substance is non-toxic trivalent chromium by the growth activity of the alkalophilic Cellulomonas genus microorganism.
  • the hexavalent chromium derived from the hydraulic substance is greatly reduced.
  • the following advantages can be obtained.
  • an alkalophilic Cellulomonas microorganism is used. This is advantageous in that hexavalent chromium can be reduced by an alkalophilic Cellulomonas microorganism even if the aqueous solution is alkaline.
  • the Cellulomonas microorganism K32A strain When used as the alkalophilic Cellulomonas microorganism, it has heat resistance. This is advantageous in that hexavalent chromium can be reduced efficiently even in a high-temperature environment when a hydraulic substance such as cement hardens.
  • curdlan is produced as ⁇ -1,3 glucan. This is advantageous in that it is possible to suppress the occurrence of cracks in the molded body after the hydraulic substance is cured, for example, concrete.
  • hexavalent chromium in the molded article is greatly reduced. Therefore, elution of hexavalent chromium from the molded body can be prevented.
  • ⁇ -1,3 glucan extracted or purified from a microorganism capable of producing ⁇ -1,3 glucan may be blended.
  • the culture medium was prepared by adding phosphoric acid to 1/5 PTYG medium. Specifically, 1 g of peptone, 1 g of tryptone, 1 g of yeast extract, 1 g of glucose, and 10 g of Na 2 HPO 4 were dissolved in water, and further water was added to finally adjust the volume to 1 L. Each medium at pH 7.5, 8.5, 9.5, 10.5, 11.5, 12.5 was adjusted using 1N NaOH as a pH adjuster, and then sterilized by filtration. Each medium was inoculated with Cellulomonas sp.
  • the Cellulomonas sp. Microorganism K32A strain exhibits a proliferation action even when the pH of the medium is 12.5. This indicates that the Cellulomonas microorganism K32A strain can grow in the presence of strong alkalinity.
  • the cellulomonas flavigena which is a neutral bacterium, has a reduced number of bacteria when the pH of the medium is pH 11.5, and no bacteria grows when the pH of the medium is 12.5. That is confirmed.
  • the culture medium is prepared by dissolving 5 g yeast extract, 17 g casein peptone, 3 g soybean peptone, 2.5 g K 2 HPO 4, 2.5 g glucose, 5 g NaCl in water, and finally adding water to a volume of 1 L. did.
  • the pH was adjusted to 7.1 to 7.5 using 1N NaOH as a pH adjuster, and then sterilized by filtration.
  • the medium was inoculated with Cellulomonas sp. Strain K32A so as to have the number of bacteria shown in Table 3 below, and cultured at 40, 45, 50, and 55 ° C. for 16 hours. After 1, 2, 4, 8, and 16 hours from the start of culture, the number of microorganisms (cfu) in 1 mL of the medium was determined by a plate smearing method. The results are shown in Table 3.
  • Peptone saline buffer was used as the culture medium. Specifically, 3.56 g of KH 2 PO 4, 7.226 g of anhydrous Na 2 HPO 4 , 4.3 g of NaCl, and 1 g of peptone were dissolved in water, and further water was added to finally prepare 1 L capacity. Hexavalent chromium was added to the medium to a concentration of 0.2 ppm. The pH was adjusted to 8.5, 10.5, and 12.5 using 1N NaOH as a pH adjuster, and then sterilized by filtration. The medium was inoculated with Cellulomonas sp. Microorganism K32A strain at 1.36 ⁇ 10 6 cfu / mL, and cultured at 30 ° C.
  • Peptone saline buffer was used as the culture medium. Specifically, 3.56 g of KH 2 PO 4, 7.226 g of anhydrous Na 2 HPO 4 , 4.3 g of NaCl, 1 g of peptone, and 2.5 g of reducing sugar are dissolved in water, and water is added to finally add 1 L capacity. Prepared. Hexavalent chromium was added to the medium to a concentration of 0.2 ppm. The pH was adjusted to 12.5 using 1N NaOH as a pH adjuster, and then sterilized by filtration. As the reducing sugar, lactose (lactose) and maltose (malt sugar) were used. A medium without reducing sugar was also prepared for comparison.
  • the medium was inoculated with Cellulomonas sp.
  • Microorganism K32A strain at 1.36 ⁇ 10 6 cfu / mL and cultured at 30 ° C. for 16 hours.
  • the concentration of hexavalent chromium in the medium was measured using JISK0102 65.2.1 (diphenylcarbazide absorptiometry).
  • JISK0102 65.2.1 diphenylcarbazide absorptiometry.
  • the results are shown in Table 6. “-” In Table 6 indicates a value below the measurement limit value.
  • Peptone saline buffer was used as the culture medium. Specifically, 3.56 g of KH 2 PO 4, 7.226 g of anhydrous Na 2 HPO 4 , 4.3 g of NaCl, and 1 g of peptone were dissolved in water, and further water was added to finally prepare 1 L capacity. Hexavalent chromium was added to the medium at a concentration of 0.2 ppm, and iron chloride (II) was added as a blast furnace slag to a concentration of 0.2 ppm. The pH was adjusted to 12.5 using 1N NaOH as a pH adjuster, and then sterilized by filtration. A medium without iron (II) chloride was also prepared for comparison.
  • the medium was inoculated with Cellulomonas sp. Strain K32A at 1.24 ⁇ 10 6 cfu / mL and cultured at 30 ° C. for 16 hours. About 2, 4, 8, and 16 hours after the start of culture, the concentration of hexavalent chromium in the medium was measured using JISK0102 65.2.1 (diphenylcarbazide absorptiometry). The results are shown in Table 8. “-” In Table 8 indicates a value below the measurement limit value. Moreover, it tested similarly using the culture medium which does not add Cellulomonas genus microorganism K32A strain
  • Peptone saline buffer was used as the culture medium. Specifically, 3.56 g of KH 2 PO 4, 7.226 g of anhydrous Na 2 HPO 4 , 4.3 g of NaCl, 1 g of peptone, and 10 g of ascorbic acid as a reducing agent are dissolved in water, and finally water is added to finally add Prepared to 1 L volume. Hexavalent chromium was added to the medium to a concentration of 0.2 ppm. The pH was adjusted to 12.5 using 1N NaOH as a pH adjuster, and then sterilized by filtration. A medium without ascorbic acid was also prepared for comparison. The medium was inoculated with Cellulomonas sp.
  • Peptone saline buffer was used as the culture medium. Specifically, 3.56 g of KH 2 PO 4, 7.226 g of anhydrous Na 2 HPO 4 , 4.3 g of NaCl, and 1 g of peptone were dissolved in water, and further water was added to finally prepare 1 L capacity. Hexavalent chromium was added to the medium to a concentration of 0.2 ppm. The pH was adjusted to 12.5 using 1N NaOH as a pH adjuster, and then sterilized by filtration.
  • Cellulomonas genus microorganism K32A strain is 1.36 ⁇ 10 5 cfu / mL, 1.36 ⁇ 10 6 cfu / mL, 1.36 ⁇ 10 7 cfu / mL, 1.36 ⁇ 10 8 cfu / mL in the medium.
  • Each was inoculated and cultured at 30 ° C. for 24 hours. After 2, 4, 8, 16, and 24 hours from the start of the culture, the concentration of hexavalent chromium in the medium was measured using 65.2.1 (diphenylcarbazide absorptiometry) of JISK0102. Moreover, it tested similarly using the culture medium which does not add Cellulomonas genus microorganism K32A strain
  • Test Example 8 Effect on strength of mortar of Cellulomonas sp. Strain K32A
  • stock was tested.
  • Portland cement manufactured by Ube Mitsubishi Cement Co., Ltd.
  • Mortar followed the manufacturing method of the test specimen of JISR5201 (physical test method of cement).
  • the components described in Table 13 were mixed, and the Cellulomonas genus microorganism K32A strain was described in Table 13 as the number of inoculated bacteria 1 ⁇ 10 3 cfu / mL, 1 ⁇ 10 6 cfu / mL, and 1 ⁇ 10 9 cfu / mL, respectively. Mixed with the amount of fungus to be used.
  • the Cellulomonas sp. Strain K32A was obtained by diluting 1 mL of a culture solution having a growth curve in a stationary phase to 1 L of water to give an inoculum number of 1 ⁇ 10 9 cfu / mL.
  • a solution obtained by further diluting the 1 ⁇ 10 9 cfu / mL bacterial solution 1000 times was used as a bacterial solution having an inoculum number of 1 ⁇ 10 6 cfu / mL.
  • a solution obtained by further diluting the 1 ⁇ 10 6 cfu / mL bacterial solution 1000 times was used as a bacterial solution having an inoculum count of 1 ⁇ 10 3 cfu / mL.
  • the preparation of the specimen was conducted according to C.C. 8.1.8 (mortar compression strength ratio test).
  • the mold was filled in a mold having a diameter of 50 mm and a length of 100 mm, and after demolding, the specimen was cured to a predetermined age in a constant temperature water bath.
  • Microorganism K32A strain were mixed in the blending amounts shown in Table 14 below to prepare a paste. After solidifying it, a hexavalent chromium elution test was conducted. Meanwhile, 350 g of Aso Lafarge Cement Co. (Nydust), 350 g of water, 1850 g of soil, and Cellulomonas sp. Microorganism K32A strain were mixed in the blending amounts shown in Table 15 below to prepare slurry. After solidifying it, a hexavalent chromium elution test was conducted. The curing days of the paste solidified product and the slurry solidified product were each 28 days.
  • Hexavalent chromium elution from paste solidified product or slurry solidified product was measured according to the Environment Agency Notification No. 46 dissolution test, and the hexavalent chromium concentration was measured based on 65.2.1 (diphenylcarbazide absorptiometric method) of JISK0102. did.
  • Table 14 shows the results of the elution amount of hexavalent chromium from the paste solidified product.
  • Table 15 shows the results of the elution amount of hexavalent chromium from the slurry solidified product.

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  • Curing Cements, Concrete, And Artificial Stone (AREA)

Abstract

La présente invention concerne un procédé de réduction du chrome hexavalent, caractérisé par la mise en contact d'un microorganisme alcaliphile appartenant au genre Cellulomonas avec un matériau hydraulique contenant du chrome hexavalent, une solution aqueuse dudit matériau hydraulique étant alcaline. Un sucre réducteur, un agent réducteur et du laitier de haut fourneau peuvent, en outre, être mélangés audit matériau hydraulique.
PCT/JP2010/059156 2010-05-28 2010-05-28 Procédé de réduction du chrome hexavalent, procédé de production d'un corps moulé et procédé d'amélioration des sols WO2011148512A1 (fr)

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JP2012517078A JP5602848B2 (ja) 2010-05-28 2010-05-28 六価クロムの還元方法、成形体の製造方法及び地盤改良方法
PCT/JP2010/059156 WO2011148512A1 (fr) 2010-05-28 2010-05-28 Procédé de réduction du chrome hexavalent, procédé de production d'un corps moulé et procédé d'amélioration des sols

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PCT/JP2010/059156 WO2011148512A1 (fr) 2010-05-28 2010-05-28 Procédé de réduction du chrome hexavalent, procédé de production d'un corps moulé et procédé d'amélioration des sols

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Publication number Priority date Publication date Assignee Title
KR101989536B1 (ko) * 2018-02-05 2019-06-14 한국광해관리공단 강환원제 또는 이의 혼합물을 세척제로 이용한 중금속 오염 토양 세척 방법

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