WO2011132197A1 - Composé efficace pour l'augmentation de la croissance reproductrice de plantes cultivées, de plantes végétales, de plantes à épices, de plantes à fleurs et de jardinage associées - Google Patents

Composé efficace pour l'augmentation de la croissance reproductrice de plantes cultivées, de plantes végétales, de plantes à épices, de plantes à fleurs et de jardinage associées Download PDF

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Publication number
WO2011132197A1
WO2011132197A1 PCT/IN2010/000489 IN2010000489W WO2011132197A1 WO 2011132197 A1 WO2011132197 A1 WO 2011132197A1 IN 2010000489 W IN2010000489 W IN 2010000489W WO 2011132197 A1 WO2011132197 A1 WO 2011132197A1
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WIPO (PCT)
Prior art keywords
plant
plants
ppm
crop
sitosterole
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PCT/IN2010/000489
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English (en)
Inventor
Chetan Balar
Anil Nakum
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Chetan Balar
Anil Nakum
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Publication date
Application filed by Chetan Balar, Anil Nakum filed Critical Chetan Balar
Publication of WO2011132197A1 publication Critical patent/WO2011132197A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring

Definitions

  • This invention is based on efficient plant growth regulator for agricultural crop plants, vegetable plants, spices plants, flower and gardening plants thereof.
  • the present invention provides a method for producing a transgenic graminaceous plant cell, said method comprising: (i) obtaining embryonic cells from a mature graminaceous grain; arid (ii) contacting said embryonic cells with a bacterium capable of transforming a plant cell, said bacterium comprising transfer-nucleic acid to be introduced into the embryonic cell!, said contacting being for a time and under conditions sufficient for said bacterium to introduce said transfer-nucleic acid into one or more of the embryonic cells, thereby producing a transgenic graminaceous plant cell.
  • the present invention also provides a method for producing a transgenic graminaceous plant.
  • the present invention also provides a transgenic graminaceous plant cell and/or a transgenic graminaceous plant produced by said method.
  • the present invention also provides a method for expressing a nucleic acid in a transgenic graminaceous plant cell or a transgenic graminaceous plant.
  • Polynucleotides which are capable of enhancing a growth, yield under water-limited conditions, and/or increased tolerance to an environmental stress of a plant transformed to contain such polynucleotides. Also provided are methods of using such polynucleotides and transgenic plants and agricultural products, including seeds, containing such polynucleotides as transgenes.
  • the present disclosure provides a plant rejuvenating agent using dehydroepiandrosterone (DHEA), or hydrates thereof, to form plant rejuvenating mixtures that may include additional components.
  • DHEA dehydroepiandrosterone
  • the plant rejuvenating mixture including DHEA may be used in typical horticultural applications, including the improvement of soil fertility; the rejuvenation of unhealthy plants by providing both water for increased turgidity and sterols for plant cell repair; enhancing the longevity of growing season of plants; and retarding the senescence of fresh cut flowers.
  • IPC8 Class: AC12N1554FI USPC Class: 800279 wherein The invention provides methods of increasing resistance to disease in a plant.
  • plant steroid biosynthesis enzyme coding sequences including sequences for squalene synthase, constructs comprising these sequences, plants transformed therewith and methods of use thereof. Methods for producing a plant with increased disease resistance' are also provided. In certain aspects of the invention, methods for producing plants transformed with the nucleic acids are provided, the transformed plants exhibiting improved disease resistance.
  • the present disclosure provides a plant rejuvenating agent using dehydroepiandrosterone (DHEA), or hydrates thereof, to form plant rejuvenating mixtures that may include additional components.
  • DHEA dehydroepiandrosterone
  • the plant rejuvenating mixture including DHEA may be used in typical horticultural applications, including the improvement of soil fertility; the rejuvenation of unhealthy plants by providing both water for increased turgidity and sterols for plant cell repair; enhancing the longevity of growing season of plants; and retarding the senescence of fresh cut flowers.
  • the present invention includes unique phylosterol compositions which comprise beta-sitosterol, campesterol and stigmastanol and/or derivatives thereof. As described further hereinbelow, these unique compositions may partially com prise tall-oil pulping soap derived phylosterols and/or phylostanols In the existing system as given in US 2005/0102716 A 1 wherein Provided are recomoinani constructs comprising DNA sequences encoding enzymes effective in altering the biosynthesis and accumulation of sterol compounds and tocopherols in transgenic plants.
  • transgenic plants seeds of which contain elevated levels of sitostanol and/or sitostanol esters, and a-tocopherol, as well as reduced levels of campesterol and campestanol and their corresponding esters. These seeds also contain the novel sterol brassicastanol. Oil obtained from seeds of such transgenic plants is also provided. This oil can be used to prepare food and pharmaceutical compositions effective in lowering the level of low density lipoprotein cholesterol in blood serum. In addition, novel DNA sequences encoding plant steroid 5a-reductases are also disclosed.
  • the present invention provides a method for producing a transgenic graminaceous plant cell, said method comprising: (i) obtaining embryonic cells from a mature graminaceous grain; and (ii) contacting said embryonic cells with a bacterium capable of transforming a plant cell, said bacterium comprising transfer-nucleic acid to be introduced into the embryonic cells, said contacting being for a time and under conditions sufficient for said bacterium to introduce said transfer-nucleic acid into one or more of the embryonic cells, thereby producing a transgenic graminaceous plant cell.
  • the present invention also provides a method for producing a transgenic graminaceous plant.
  • the present invention also provides a transgenic graminaceous plant cell and/or a transgenic graminaceous plant produced by said method.
  • the present invention also provides a method for expressing a nucleic acid in a transgenic graminaceous plant cell or a transgenic graminaceous plant.
  • the main object of the invention is to introduce the application of compound of the class Phytosterol with special emphasis on compound b-sitosterol and its derivatives for enhanced crop yield, which occur naturally in some plants and can be extracted from them.
  • fertilizers can also cause increased yield from plants, they have to be synthesized chemically and are not a natural product, also fertilizers are relatively expensive to use.
  • the introduced group of compound are cost effective as compared to other existing products, thus the application of these compounds as an aid to agriculture has a very positive economic impact.
  • the group of compound phytosterol and especially b-sitosterol and its derivatives are more effective to others because they significantly and rapidly increase flowering in plants. Also these compounds are absolutely harmless for balance plant physiology.
  • This invention is based on natural compound which are efficient growth regulator for agriculture crop. These compounds are of the class phytosterol and especially b-sitosterol and its derivatives. These compounds are more effective to other agricultural growth fegulators because they significantly enhance the reproductive growth of the crop by rapidly increasing flowering in crop plants, vegetable plants, spices plants, flower and gardening plants thereof.
  • This invention is based on natural compound which are efficient growth regulator for agriculture crop. These compounds are of the class phytosterol and especially b-sitosterol and its derivatives. These compounds are more effective to other agricultural growth regulators because they
  • Phytosterols are a group of steroid alcohols, phytochemicals naturally occurring in plants. Phytosterol occur naturally in small quantities in vegetable oils, especially sea buckthorn oil (1640 mg/lOOg oil), corn oil (968 mg/lOOg), and soybean oil (327 mg/lOOg oil) and are thus absolutely harmless.
  • Certain substances are known to increase and regulate the crop growth. Each of these substances enhances the crop yield by different mechanisms.
  • plant growth regulators mechanism are germination enhancers, root stimulants, plant stature controllers, plant maturity agents, ripening agents and plant dormancy agents. These are used to obtain better quantity and better yield of the crop plants, vegetable plants, spices plants, flower and gardening plants thereof.
  • germination enhancers cause rapid emergence of the plant from the seed, and thereby allow better weed control around the plant.
  • Phytosterols and specially b-sitosterol and its derivatives which occur naturally in some plants enhance the crop yield by stimulating the flowering in the crop plants.
  • Fertilizers are basically nutrients for the crop growth and normally do not enhance and regulate the yield of the crop on the mechanisms mentioned above.
  • B-sitosterol or ⁇ -Sitosterol is one of several phytosterol (plant sterols) with chemical structures similar to that of cholesterol.
  • the natural compound phytosterol that comprises of b-sitosterol enhances the crop quality and yield by increasing leaf area, leaf number, leaf chlorophyll of leaf, canopy of plant, canopy of root like plant parts to cause over all growth and development of plant, it has a very positive and special effect during the flowering stage of the crop and induces or increases flowering significantly. It also helps to increasing health of reproductive organ like buds, fruits filling rate, fruits size.
  • the compound has been found to be extremely effective in increasing the quality and yield through the foliar application to flowering plant like chili and tomato at flowering stage, application to other crop has a similar effect as well.
  • the compound b-sitosterole was found to be much more potent and effective in comparison to the auxins. The same quantification was also used for drenching the crop which gives more potent effect compare to auxin IBA at 100 ppm.
  • B-sitosterole is a plant growth regulator and not plant hormone and thereby it can used in a broad range of concentration varying from lOOOppm to 10,000ppm, and is found to be non toxic to the plant physiology.
  • the compound is very safe and can handled very easily during the application for the foliar application.
  • Another significant advantage of the compound is its relatively low cost and thus positive impact on the agricultural economics.

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention porte sur un régulateur efficace de croissance de plantes pour la floraison de plantes cultivées, de plantes végétales, de plantes à épices, de plantes à fleurs et de jardinage associées. Le composé s'est révélé être extrêmement efficace pour l'augmentation de la qualité et du rendement à travers l'application foliaire à une plante à fleurs, telle que le chili et la tomate, au stade de floraison, l'application à une autre culture ayant également un effet similaire. Lors d'une vérification comparative pour l'effet à travers l'application foliaire à une plante à fleurs, telle que le chili et la tomate, au stade de floraison de b-sitostérol à 1000 ppm dans de l'eau DM, contre du brassinolide d'hormone naturel à 0,1 ppm, du NAA à 10 ppm, du IAA à 10 ppm, du GA3 à 500 ppm, de la Kinétine à 500 ppm. Le composé b-sitostérol s'est révélé être beaucoup plus puissant et efficace en comparaison avec les auxines. La même quantification a été également utilisée pour tremper la culture qui offre un effet plus puissant par rapport à l'auxine IBA à 100 ppm. En outre, le b-sitostérol est un régulateur de croissance de plantes et non pas une hormone de plantes et ainsi, il peut être utilisé dans une large plage de concentration variant de 1000 ppm à 10000 ppm, et s'est révélé être non toxique pour la physiologie des plantes. Le composé est très sûr et peut être manipulé très facilement pendant l'application pour l'application foliaire. Un autre avantage significatif du composé est son coût relativement faible et par conséquent, son impact positif sur l'économie agricole.
PCT/IN2010/000489 2010-04-20 2010-07-28 Composé efficace pour l'augmentation de la croissance reproductrice de plantes cultivées, de plantes végétales, de plantes à épices, de plantes à fleurs et de jardinage associées WO2011132197A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1286MU2010 2010-04-20
IN1286/MUM/2010 2010-04-20

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102613009A (zh) * 2012-03-29 2012-08-01 常熟市佳盛农业科技发展有限公司 一种树木嫁接专用营养液
CN102992902A (zh) * 2012-11-30 2013-03-27 王正泽 一种植物生长促进剂的制备方法
CN102992903A (zh) * 2012-11-30 2013-03-27 王正泽 一种植物生长促进剂
CN103262849A (zh) * 2013-04-23 2013-08-28 青岛农业大学 一种植物甾醇农用杀菌剂、制备方法及其应用
FR3069756A1 (fr) * 2017-08-07 2019-02-08 Innovi Production Tensioactif non ionique comme biostimulant
CN110199787A (zh) * 2019-05-21 2019-09-06 广东东阳光药业有限公司 一种同时调控珠芽蓼开花和结珠芽的方法

Citations (9)

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US4346226A (en) 1980-08-28 1982-08-24 The United States Of America As Represented By The Secretary Of The Department Of Agriculture Plant growth promoting brassinosteroids
EP0200371A1 (fr) * 1985-04-02 1986-11-05 The Director, Fruit Tree Research Station, Ministry Of Agriculture, Forestry And Fisheries, Government Of Japan Agent et procédé pour élaguer des fleurs des arbres fruitiers
JPH0789808A (ja) * 1993-09-24 1995-04-04 Mitsui Norin Kk 植物病害防護剤
US20040259732A1 (en) * 2003-04-28 2004-12-23 Monsanto Technology, L.L.C. Treatment of plants and plant propagation materials with an antioxidant to improve plant health and/or yield
US20050102716A1 (en) 1999-04-12 2005-05-12 Mylavarapu Venkatramesh Transgenic plants containing altered levels of sterol compounds and tocopherols
US20060178269A1 (en) * 2005-02-08 2006-08-10 Medina-Vega Luis R Plant conditioning treatment for plant growth and health enhancement
WO2009068588A2 (fr) 2007-11-27 2009-06-04 Basf Plant Science Gmbh Plantes transgéniques présentant une tolérance au stress et un rendement accrus
US20090191636A1 (en) 2006-01-11 2009-07-30 Ramage Carl Mcdonald Method of Producing Transgenic Graminaceous Cells and Plants
US7608126B1 (en) 2006-04-04 2009-10-27 Starcher Robert C Method for horticulture plant rejuvenation

Patent Citations (9)

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US4346226A (en) 1980-08-28 1982-08-24 The United States Of America As Represented By The Secretary Of The Department Of Agriculture Plant growth promoting brassinosteroids
EP0200371A1 (fr) * 1985-04-02 1986-11-05 The Director, Fruit Tree Research Station, Ministry Of Agriculture, Forestry And Fisheries, Government Of Japan Agent et procédé pour élaguer des fleurs des arbres fruitiers
JPH0789808A (ja) * 1993-09-24 1995-04-04 Mitsui Norin Kk 植物病害防護剤
US20050102716A1 (en) 1999-04-12 2005-05-12 Mylavarapu Venkatramesh Transgenic plants containing altered levels of sterol compounds and tocopherols
US20040259732A1 (en) * 2003-04-28 2004-12-23 Monsanto Technology, L.L.C. Treatment of plants and plant propagation materials with an antioxidant to improve plant health and/or yield
US20060178269A1 (en) * 2005-02-08 2006-08-10 Medina-Vega Luis R Plant conditioning treatment for plant growth and health enhancement
US20090191636A1 (en) 2006-01-11 2009-07-30 Ramage Carl Mcdonald Method of Producing Transgenic Graminaceous Cells and Plants
US7608126B1 (en) 2006-04-04 2009-10-27 Starcher Robert C Method for horticulture plant rejuvenation
WO2009068588A2 (fr) 2007-11-27 2009-06-04 Basf Plant Science Gmbh Plantes transgéniques présentant une tolérance au stress et un rendement accrus

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MATHUR, N. AND VYAS, A.: "Physiological effect of some bioregulators on vegetative growth, yield and chemical constituents of pearl millet (Pennisetum typhoides (Burm) Stapf. and Hubb)", INTERNATIONAL JOUKRNAL OF AGRICULTURAL RESEARCH, vol. 2, no. 3, 2007, pages 238 - 245, XP007917051, ISSN: 1816-4897, DOI: 10.3923/ijar.2007.238.245 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102613009A (zh) * 2012-03-29 2012-08-01 常熟市佳盛农业科技发展有限公司 一种树木嫁接专用营养液
CN102992902A (zh) * 2012-11-30 2013-03-27 王正泽 一种植物生长促进剂的制备方法
CN102992903A (zh) * 2012-11-30 2013-03-27 王正泽 一种植物生长促进剂
CN103262849A (zh) * 2013-04-23 2013-08-28 青岛农业大学 一种植物甾醇农用杀菌剂、制备方法及其应用
FR3069756A1 (fr) * 2017-08-07 2019-02-08 Innovi Production Tensioactif non ionique comme biostimulant
CN110199787A (zh) * 2019-05-21 2019-09-06 广东东阳光药业有限公司 一种同时调控珠芽蓼开花和结珠芽的方法
CN110199787B (zh) * 2019-05-21 2021-09-28 东莞市东阳光冬虫夏草研发有限公司 一种同时调控珠芽蓼开花和结珠芽的方法

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