WO2011113977A1 - Biomass production method using aqueous aerosols containing photosynthetic microorganisms - Google Patents

Biomass production method using aqueous aerosols containing photosynthetic microorganisms Download PDF

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Publication number
WO2011113977A1
WO2011113977A1 PCT/ES2011/000082 ES2011000082W WO2011113977A1 WO 2011113977 A1 WO2011113977 A1 WO 2011113977A1 ES 2011000082 W ES2011000082 W ES 2011000082W WO 2011113977 A1 WO2011113977 A1 WO 2011113977A1
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Prior art keywords
suspension
photosynthetic microorganisms
aerosols
atmospheric
biomass production
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PCT/ES2011/000082
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Spanish (es)
French (fr)
Inventor
Alfonso Miguel GAÑÁN CALVO
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Universidad De Sevilla
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Publication of WO2011113977A1 publication Critical patent/WO2011113977A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/002Photo bio reactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor

Definitions

  • the present invention falls within the technical field of biomass production for various uses and the removal of atmospheric C0 2 .
  • Said production is proposed to be carried out in an open air environment and exposed to solar radiation, where the air can freely enter and exit, but in which the entry or exit of aerosols can be restricted by any method.
  • a suspension of micro-algae or other photosynthetic microorganisms is permanently nebulized in the form of drops containing one or more of said microorganisms, such that during the time that the microdrops are kept in the air, they receive radiation solar and are in contract with the atmospheric C0 2 , carrying out a biomass generation process that is characterized by its high irradiation rate and surface contact with air per unit volume.
  • An object of the present invention is a biomass production method by using aqueous aerosols containing photosynthetic microorganisms. Aerosols are produced from an aqueous suspension of said microorganisms that may have the necessary nutrients for their development. Said aerosols are released in an atmospheric air environment subjected to solar radiation, and they remain suspended long enough for the biomass production yield to be positive. For this, the aerosol is released in a way that describes a global trajectory that maximizes said maintenance time in suspension, under the action of gravitational and hydrodynamic forces within the air. Additionally, forced air currents may or may not be established in the environment, or favor natural ones, so that the suspension time increases and the energy efficiency of the system is maintained within the parameters of sustainability.
  • An object of the present invention is also an environment for the production of photosynthetic microorganisms by aerosols in air suspension obtained from an aqueous suspension of said microorganisms that may contain nutrients for their development.
  • Said aqueous suspension may contain one or several different species and may also receive sedimented aerosols.
  • ecosystems can also develop that may or may not reach a permanent regime, so that they can be favored by some type of artificial action to produce a specific species or biological product desired.
  • Another object of the present invention is a method of cultivating photosynthetic microorganisms and producing biomass by means of suspended aerosols as described above, to remove atmospheric C0 or a certain natural or artificial effluent.
  • FIG 1. Shows a schematic representation of the basic biomass production cycle proposed in the present invention.
  • FIG 2. Shows a schematic representation of an aerial artificial forest, object of the present invention.
  • the basic biomass production cycle proposed in the present invention comprises a stage of nebulization of an aqueous suspension (1) of photosynthetic microorganisms (2) that, optionally, may comprise the nutrients necessary for the development of microorganisms
  • These aerosols (1) have been released in an atmospheric air environment subjected to solar radiation (3) and are kept in suspension long enough for the biomass production yield to be positive.
  • aerosols with photosynthetic microorganisms (2) that, optionally, may comprise the nutrients necessary for the biomass production yield to be positive.
  • the aerosols (1) with the photosynthetic microorganisms (2) settle (4) and are collected (5) in such a way that a feedback (6) is established with the original culture, to be again fogged.
  • the aerosols (1) are released in such a way that they describe an upward and downward trajectory that maximizes the maintenance time in suspension, under the action of gravitational and hydrodynamic forces within the air.
  • the aqueous suspension may contain one or several different species of biological microorganisms and a combination of the sedimented aerosols, as indicated above, as well as selected nutrients for the development of said photosynthetic microorganisms and other biological organisms that are in the aqueous suspension. .
  • the nebulization process is maintained for very long periods of time compared to the typical air suspension times of an aerosol drop, whereby said environment generates repetitive biochemical cycles that can favor, enhance or establish selective biological developments, thus favoring selective to certain species or the generation of certain biological products, and that can develop ecosystems that may or may not reach a permanent regime.
  • the generated ecosystems can be favored by any type of artificial action (that is, a cultivation action) of the control group of temperature, humidity, pH of aqueous suspension, concentration of nitrogen oxides, sulfur, iron and salts of sodium, potassium, calcium and magnesium, so that, as indicated, the development of a certain species or species is favored, as well as the generation of a specific biological product.
  • Figure 2 shows an example of practical application of the invention in an artificial forest, where a culture of suspended algae and nutrients (21) is permanently sprayed (22) in the form of an aerosol, generating a cloud (23) that absorbs solar radiation (24) and C0 2 (25) generating a high biomass yield, since the aerosol that precipitates (that is, sediments) is collected in the crop (21) and nebulized again.

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a biomass production method using aqueous aerosols containing photosynthetic microorganisms, including: a first step comprising the production of an aerosol (1) consisting of an aqueous suspension of photosynthetic microorganisms; and a second step comprising the release of the aerosol into an atmospheric air environment subjected to solar radiation (3), such that said suspension is maintained for a sufficient amount of time in order for a positive biomass production performance to be obtained, said suspension receiving solar radiation and being in contact with the atmospheric CO2, thereby providing a natural biomass generation process.

Description

PROCEDIMIENTO DE PRODUCCIÓN DE BIOMASA MEDIANTE EL USO DE AEROSOLES ACUOSOS QUE CONTIENEN MICROORGANISMOS BIOMASS PRODUCTION PROCEDURE THROUGH THE USE OF WATERPROOF AEROSOLS CONTAINING MICROORGANISMS
FOTOSINTÉTICOS PHOTOSYNTHETICS
La presente invención se encuadra dentro del campo técnico de la producción de biomasa para diversos usos y la retirada de C02 atmosférico. Dicha producción se propone realizarla en un entorno aéreo abierto y expuesto a la radiación solar, en donde pueda libremente entrar y salir el aire, pero en el que se pueda restringir la entrada o salida de aerosoles mediante cualquier método. En el entorno descrito se nebuliza de forma permanente una suspensión de micro-algas u otros microorganismos fotosintéticos en forma de gotas que contienen uno o varios de dichos microorganismos, de tal forma que durante el tiempo que se mantienen en el aire las microgotas, reciban radiación solar y estén en contracto con el C02 atmosférico, realizando un proceso de generación de biomasa que se caracteriza por su elevada tasa de irradiación y superficie de contacto con el aire por unidad de volumen. The present invention falls within the technical field of biomass production for various uses and the removal of atmospheric C0 2 . Said production is proposed to be carried out in an open air environment and exposed to solar radiation, where the air can freely enter and exit, but in which the entry or exit of aerosols can be restricted by any method. In the described environment, a suspension of micro-algae or other photosynthetic microorganisms is permanently nebulized in the form of drops containing one or more of said microorganisms, such that during the time that the microdrops are kept in the air, they receive radiation solar and are in contract with the atmospheric C0 2 , carrying out a biomass generation process that is characterized by its high irradiation rate and surface contact with air per unit volume.
ESTADO DE LA TÉCNICA ANTERIOR STATE OF THE PREVIOUS TECHNIQUE
Se desconoce por parte del inventor, experto en la materia de ningún método o procedimiento que contenga las características esenciales de la invención tal y como se describen a continuación. It is unknown by the inventor, skilled in the art of any method or procedure that contains the essential characteristics of the invention as described below.
EXPLICACIÓN DE LA INVENCIÓN EXPLANATION OF THE INVENTION
Es un objeto de la presente invención un método de producción de biomasa mediante el uso de aerosoles acuosos que contienen microorganismos fotosintéticos. Los aerosoles son producidos a partir de una suspensión acuosa de dichos microorganismos que pueden tener los nutrientes necesarios para su desarrollo. Dichos aerosoles son liberados en un entorno aéreo atmosférico sometido a la radiación solar, y se mantienen en suspensión el tiempo suficiente para que el rendimiento de producción de biomasa resulte positivo. Para ello, el aerosol se libera de forma que describa una trayectoria global que maximice dicho tiempo de mantenimiento en suspensión, bajo la acción de las fuerzas gravitatorias e hidrodinámicas en el seno del aire. Adicionalmente, se pueden o no establecer corrientes forzadas de aire en el entorno, o favorecer las naturales, de forma que aumente el tiempo de suspensión y que el rendimiento energético del sistema se mantenga dentro de los parámetros de la sostenibilidad. Es también un objeto de la presente invención un entorno de producción de microorganismos fotosintéticos mediante aerosoles en suspensión aérea obtenidos a partir de una suspensión acuosa de dichos microorganismos que puede contener nutrientes para su desarrollo. Dicha suspensión acuosa puede contener una o varias especies diversas y puede recibir también los aerosoles sedimentados. An object of the present invention is a biomass production method by using aqueous aerosols containing photosynthetic microorganisms. Aerosols are produced from an aqueous suspension of said microorganisms that may have the necessary nutrients for their development. Said aerosols are released in an atmospheric air environment subjected to solar radiation, and they remain suspended long enough for the biomass production yield to be positive. For this, the aerosol is released in a way that describes a global trajectory that maximizes said maintenance time in suspension, under the action of gravitational and hydrodynamic forces within the air. Additionally, forced air currents may or may not be established in the environment, or favor natural ones, so that the suspension time increases and the energy efficiency of the system is maintained within the parameters of sustainability. An object of the present invention is also an environment for the production of photosynthetic microorganisms by aerosols in air suspension obtained from an aqueous suspension of said microorganisms that may contain nutrients for their development. Said aqueous suspension may contain one or several different species and may also receive sedimented aerosols.
También se considera un objeto de la presente invención un entorno considerado como un bosque artificial en el que el proceso de nebulización permanente genera ciclos bioquímicos repetitivos que pueden favorecer o establecer desarrollos direccionales, favoreciendo de forma selectiva a ciertas especies. En dicho entorno pueden también desarrollarse ecosistemas que pueden llegar, o no, a un régimen permanente, de manera que pueden ser favorecidos por algún tipo de acción artificial para que produzcan una determinada especie o producto biológico deseado. It is also considered an object of the present invention an environment considered as an artificial forest in which the permanent nebulization process generates repetitive biochemical cycles that can favor or establish directional developments, selectively favoring certain species. In this environment, ecosystems can also develop that may or may not reach a permanent regime, so that they can be favored by some type of artificial action to produce a specific species or biological product desired.
Otro objeto de la presente invención es un método de cultivo de microorganismos fotosintéticos y de producción de biomasa mediante aerosoles en suspensión como el descrito anteriormente, para retirar C0 atmosférico o de un cierto efluente natural o artificial. Another object of the present invention is a method of cultivating photosynthetic microorganisms and producing biomass by means of suspended aerosols as described above, to remove atmospheric C0 or a certain natural or artificial effluent.
A lo largo de la descripción y las reivindicaciones la palabra "comprende" y sus variantes no pretenden excluir otras características técnicas, aditivos, componentes o pasos. Para los expertos en la materia, otros objetos, ventajas y características de la invención se desprenderán en parte de la descripción y en parte de la práctica de la invención. Los siguientes ejemplos y dibujos se proporcionan a modo de ilustración, y no se pretende que sean limitativos de la presente invención. Además, la presente invención cubre todas las posibles combinaciones de realizaciones particulares y preferidas aquí indicadas. Throughout the description and the claims the word "comprises" and its variants are not intended to exclude other technical characteristics, additives, components or steps. For those skilled in the art, other objects, advantages and features of the invention will be derived partly from the description and partly from the practice of the invention. The following examples and drawings are provided by way of illustration, and are not intended to be limiting of the present invention. In addition, the present invention covers all possible combinations of particular and preferred embodiments indicated herein.
BREVE DESCRIPCIÓN DE LOS DIBUJOS BRIEF DESCRIPTION OF THE DRAWINGS
FIG 1. Muestra una representación esquematizada del ciclo básico de producción de biomasa propuesto en la presente invención. FIG 1. Shows a schematic representation of the basic biomass production cycle proposed in the present invention.
FIG 2. Muestra una representación esquematizada de un bosque artificial aéreo, objeto de la presente invención. EXPOSICIÓN DETALLADA DE MODOS DE REALIZACIÓN Y EJEMPLOS FIG 2. Shows a schematic representation of an aerial artificial forest, object of the present invention. DETAILED EXHIBITION OF EMBODIMENTS AND EXAMPLES
Tal y como muestra la figura 1 el ciclo básico de producción de biomasa propuesto en la presente invención comprende una etapa de nebulización de una suspensión acuosa (1) de microorganismos fotosintéticos (2) que, opcionalmente, pueden comprender los nutrientes necesarios para el desarrollo de los microorganismos. Estos aerosoles (1) han sido liberados en un entorno aéreo atmosférico sometido a la radiación solar (3) y se mantienen en suspensión el tiempo suficiente para que el rendimiento de producción de biomasa resulte positivo. Posteriormente, los aerosoles con los microorganismos fotosintéticos (2) que, opcionalmente, pueden comprender los nutrientes necesarios para que el rendimiento de producción de biomasa resulte positivo. Posteriormente, los aerosoles (1) con los microorganismos fotosintéticos (2) sedimentan (4) y son recogidos (5) de tal forma que se establece una realimentación (6) con el cultivo original, para volver a ser nebulizado. As Figure 1 shows, the basic biomass production cycle proposed in the present invention comprises a stage of nebulization of an aqueous suspension (1) of photosynthetic microorganisms (2) that, optionally, may comprise the nutrients necessary for the development of microorganisms These aerosols (1) have been released in an atmospheric air environment subjected to solar radiation (3) and are kept in suspension long enough for the biomass production yield to be positive. Subsequently, aerosols with photosynthetic microorganisms (2) that, optionally, may comprise the nutrients necessary for the biomass production yield to be positive. Subsequently, the aerosols (1) with the photosynthetic microorganisms (2) settle (4) and are collected (5) in such a way that a feedback (6) is established with the original culture, to be again fogged.
Dentro de esta realización, los aerosoles (1) se liberan de tal forma que describen una trayectoria ascendente y descendente que maximiza el tiempo de mantenimiento en suspensión, bajo la acción de las fuerzas gravitatorias e hidrodinámicas en el seno del aire. Within this embodiment, the aerosols (1) are released in such a way that they describe an upward and downward trajectory that maximizes the maintenance time in suspension, under the action of gravitational and hydrodynamic forces within the air.
En una realización particular, es posible establecer corrientes forzadas en el entorno aéreo, o favorecer las naturales, que aumentan el tiempo de suspensión de los aerosoles frente al resultante de la sedimentación en una atmósfera estática. In a particular embodiment, it is possible to establish forced currents in the air environment, or to favor natural ones, which increase the suspension time of aerosols compared to the result of sedimentation in a static atmosphere.
La suspensión acuosa puede contener una o varias especies diversas de microorganismos biológicos y una combinación de los aerosoles sedimentados, como anteriormente se ha indicado, así como nutrientes seleccionados para el desarrollo de dichos microorganismos fotosintéticos y de otros organismos biológicos que se encuentren en la suspensión acuosa. The aqueous suspension may contain one or several different species of biological microorganisms and a combination of the sedimented aerosols, as indicated above, as well as selected nutrients for the development of said photosynthetic microorganisms and other biological organisms that are in the aqueous suspension. .
El proceso de nebulización es mantenido durante periodos de tiempo muy largos en comparación con los tiempos típicos de suspensión aérea de una gota de aerosol, con lo cual dicho entorno genera ciclos bioquímicos repetitivos que pueden favorecer, potenciar o establecer desarrollos biológicos selectivos, favoreciendo de manera selectiva a ciertas especies o la generación de productos biológicos determinados, y que pueden desarrollar ecosistemas que pueden alcanzar o no un régimen permanente. Los ecosistemas generados pueden ser favorecidos por cualquier tipo de acción de artificial (es decir, una acción de cultivo) del grupo de control de temperatura, grado de humedad, pH de suspensión acuosa, concentración de óxidos de nitrógeno, azufre, hierro y sales de sodio, potasio, calcio y magnesio, para que, como se ha indicado, se favorezca el desarrollo de una determinada especie o especies, así como la generación de un producto biológico determinado. The nebulization process is maintained for very long periods of time compared to the typical air suspension times of an aerosol drop, whereby said environment generates repetitive biochemical cycles that can favor, enhance or establish selective biological developments, thus favoring selective to certain species or the generation of certain biological products, and that can develop ecosystems that may or may not reach a permanent regime. The generated ecosystems can be favored by any type of artificial action (that is, a cultivation action) of the control group of temperature, humidity, pH of aqueous suspension, concentration of nitrogen oxides, sulfur, iron and salts of sodium, potassium, calcium and magnesium, so that, as indicated, the development of a certain species or species is favored, as well as the generation of a specific biological product.
Ejemplo Example
En la figura 2 se muestra un ejemplo de aplicación práctica de la invención en un bosque artificial, donde un cultivo de algas en suspensión y nutrientes (21) es nebulizado (22) permanentemente en forma de aerosol, generando una nube (23) que absorbe la radiación solar (24) y el C02 (25) generando un alto rendimiento de biomasa, puesto que el aerosol que precipita (es decir, que sedimenta) es recogido en el cultivo (21) y nebulizado nuevamente. Figure 2 shows an example of practical application of the invention in an artificial forest, where a culture of suspended algae and nutrients (21) is permanently sprayed (22) in the form of an aerosol, generating a cloud (23) that absorbs solar radiation (24) and C0 2 (25) generating a high biomass yield, since the aerosol that precipitates (that is, sediments) is collected in the crop (21) and nebulized again.

Claims

REIVINDICACIONES
1. - Método de producción de biomasa mediante el uso de aerosoles acuosos que contienen microorganismos fotosintéticos que se caracteriza porque comprende una primera etapa de producción de aerosol (1) consistente en una suspensión acuosa de microorganismos fotosintéticos; y una segunda etapa de liberación del aerosol en un entorno aéreo atmosférico sometido a la radiación solar (3), de tal forma que dicha suspensión se mantenga el tiempo suficiente para que el rendimiento de producción de biomasa resulte positivo, recibiendo radiación solar y estando en contacto con el C02 atmosférico, realizando un proceso natural de generación de biomasa. 1. - Method of biomass production through the use of aqueous aerosols containing photosynthetic microorganisms characterized in that it comprises a first stage of aerosol production (1) consisting of an aqueous suspension of photosynthetic microorganisms; and a second stage of aerosol release in an atmospheric air environment subjected to solar radiation (3), such that said suspension is maintained long enough for the biomass production yield to be positive, receiving solar radiation and being in contact with atmospheric C0 2 , carrying out a natural biomass generation process.
2. - Método de acuerdo con la reivindicación 1 que se caracteriza porque los aerosoles (1) se liberan describiendo una trayectoria descendente, o ascendente- descendente, maximizando el tiempo de mantenimiento en suspensión bajo la acción de las fuerzas gravitatorias e hidrodinámicas en el seno del aire. 2. - Method according to claim 1 characterized in that the aerosols (1) are released describing a downward, or ascending-downward path, maximizing the maintenance time in suspension under the action of gravitational and hydrodynamic forces within from air.
3. - Método de acuerdo con las reivindicaciones 1 y 2 que se caracteriza porque se establecen corrientes de aire forzadas en el entorno aéreo, favoreciendo las naturales, de tal forma que se maximice el tiempo de suspensión frente al resultante de la sedimentación en una atmósfera estática. 3. - Method according to claims 1 and 2 characterized in that forced air currents are established in the air environment, favoring the natural ones, in such a way that the suspension time is maximized compared to the result of sedimentation in an atmosphere static
4. - Método de acuerdo con las reivindicaciones anteriores, que se caracteriza porque la suspensión acuosa comprende, al menos, un componente seleccionado entre: (a) al menos una especie de organismo biológico; (b) aerosoles sedimentados; (c) una pluralidad de nutrientes seleccionados para el desarrollo de los microorganismos fotosintéticos; (d) nutrientes seleccionados para el desarrollo de otros organismos biológicos; o (e) una combinación de alguno de los anteriores. 4. - Method according to the preceding claims, characterized in that the aqueous suspension comprises at least one component selected from: (a) at least one species of biological organism; (b) sedimented aerosols; (c) a plurality of nutrients selected for the development of photosynthetic microorganisms; (d) nutrients selected for the development of other biological organisms; or (e) a combination of any of the above.
5. - Uso del método de las reivindicaciones 1 a 4 para la retirada del C02 atmosférico o procedente de un efluente artificial o natural. 5. - Use of the method of claims 1 to 4 for the removal of atmospheric C0 2 or from an artificial or natural effluent.
PCT/ES2011/000082 2010-03-18 2011-03-18 Biomass production method using aqueous aerosols containing photosynthetic microorganisms WO2011113977A1 (en)

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Citations (4)

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Publication number Priority date Publication date Assignee Title
SU501719A1 (en) * 1974-04-10 1976-02-05 Республиканский Проектный Институт "Узгипросельстрой" Plant for growing microalgae
SU1055435A1 (en) * 1981-06-19 1983-11-23 Белорусский Научно-Исследовательский Проектно-Конструкторский Институт Рыбного Хозяйства Installation for growing microalgae
US20080178739A1 (en) * 2006-07-10 2008-07-31 Greenfuel Technologies Corp. Photobioreactor systems and methods for treating CO2-enriched gas and producing biomass
WO2009134114A1 (en) * 2008-04-30 2009-11-05 Tet Shin Ho An apparatus for mass cultivation of micro algae and a method for cultivating the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU501719A1 (en) * 1974-04-10 1976-02-05 Республиканский Проектный Институт "Узгипросельстрой" Plant for growing microalgae
SU1055435A1 (en) * 1981-06-19 1983-11-23 Белорусский Научно-Исследовательский Проектно-Конструкторский Институт Рыбного Хозяйства Installation for growing microalgae
US20080178739A1 (en) * 2006-07-10 2008-07-31 Greenfuel Technologies Corp. Photobioreactor systems and methods for treating CO2-enriched gas and producing biomass
WO2009134114A1 (en) * 2008-04-30 2009-11-05 Tet Shin Ho An apparatus for mass cultivation of micro algae and a method for cultivating the same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZEBIB, T.: "Microalgae Grown in Photobioreactors for Mass Production of Biofuel", MICROALGAE GROWN IN PHOTOBIOREACTORS FOR MASS PRODUCTION OF BIOFUEL, 30 September 2008 (2008-09-30), RUTGERS UNIVERSITY. DEPARTMENT OF BIOENVIRONMENTAL ENGINEERING, Retrieved from the Internet <URL:http://www.water.rutgers.edu/Educational_Programs/Senior%20Design2008/Algae%20to%20Energy%20Report.pdf> [retrieved on 20110705] *

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