WO2011113977A1 - Procédé de production de biomasse à travers l'utilisation d'aérosols aqueux qui contiennent des micro-organismes photosynthétiques - Google Patents

Procédé de production de biomasse à travers l'utilisation d'aérosols aqueux qui contiennent des micro-organismes photosynthétiques Download PDF

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Publication number
WO2011113977A1
WO2011113977A1 PCT/ES2011/000082 ES2011000082W WO2011113977A1 WO 2011113977 A1 WO2011113977 A1 WO 2011113977A1 ES 2011000082 W ES2011000082 W ES 2011000082W WO 2011113977 A1 WO2011113977 A1 WO 2011113977A1
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WO
WIPO (PCT)
Prior art keywords
suspension
photosynthetic microorganisms
aerosols
atmospheric
biomass production
Prior art date
Application number
PCT/ES2011/000082
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English (en)
Spanish (es)
Inventor
Alfonso Miguel GAÑÁN CALVO
Original Assignee
Universidad De Sevilla
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad De Sevilla filed Critical Universidad De Sevilla
Publication of WO2011113977A1 publication Critical patent/WO2011113977A1/fr

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/002Photo bio reactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor

Definitions

  • the present invention falls within the technical field of biomass production for various uses and the removal of atmospheric C0 2 .
  • Said production is proposed to be carried out in an open air environment and exposed to solar radiation, where the air can freely enter and exit, but in which the entry or exit of aerosols can be restricted by any method.
  • a suspension of micro-algae or other photosynthetic microorganisms is permanently nebulized in the form of drops containing one or more of said microorganisms, such that during the time that the microdrops are kept in the air, they receive radiation solar and are in contract with the atmospheric C0 2 , carrying out a biomass generation process that is characterized by its high irradiation rate and surface contact with air per unit volume.
  • An object of the present invention is a biomass production method by using aqueous aerosols containing photosynthetic microorganisms. Aerosols are produced from an aqueous suspension of said microorganisms that may have the necessary nutrients for their development. Said aerosols are released in an atmospheric air environment subjected to solar radiation, and they remain suspended long enough for the biomass production yield to be positive. For this, the aerosol is released in a way that describes a global trajectory that maximizes said maintenance time in suspension, under the action of gravitational and hydrodynamic forces within the air. Additionally, forced air currents may or may not be established in the environment, or favor natural ones, so that the suspension time increases and the energy efficiency of the system is maintained within the parameters of sustainability.
  • An object of the present invention is also an environment for the production of photosynthetic microorganisms by aerosols in air suspension obtained from an aqueous suspension of said microorganisms that may contain nutrients for their development.
  • Said aqueous suspension may contain one or several different species and may also receive sedimented aerosols.
  • ecosystems can also develop that may or may not reach a permanent regime, so that they can be favored by some type of artificial action to produce a specific species or biological product desired.
  • Another object of the present invention is a method of cultivating photosynthetic microorganisms and producing biomass by means of suspended aerosols as described above, to remove atmospheric C0 or a certain natural or artificial effluent.
  • FIG 1. Shows a schematic representation of the basic biomass production cycle proposed in the present invention.
  • FIG 2. Shows a schematic representation of an aerial artificial forest, object of the present invention.
  • the basic biomass production cycle proposed in the present invention comprises a stage of nebulization of an aqueous suspension (1) of photosynthetic microorganisms (2) that, optionally, may comprise the nutrients necessary for the development of microorganisms
  • These aerosols (1) have been released in an atmospheric air environment subjected to solar radiation (3) and are kept in suspension long enough for the biomass production yield to be positive.
  • aerosols with photosynthetic microorganisms (2) that, optionally, may comprise the nutrients necessary for the biomass production yield to be positive.
  • the aerosols (1) with the photosynthetic microorganisms (2) settle (4) and are collected (5) in such a way that a feedback (6) is established with the original culture, to be again fogged.
  • the aerosols (1) are released in such a way that they describe an upward and downward trajectory that maximizes the maintenance time in suspension, under the action of gravitational and hydrodynamic forces within the air.
  • the aqueous suspension may contain one or several different species of biological microorganisms and a combination of the sedimented aerosols, as indicated above, as well as selected nutrients for the development of said photosynthetic microorganisms and other biological organisms that are in the aqueous suspension. .
  • the nebulization process is maintained for very long periods of time compared to the typical air suspension times of an aerosol drop, whereby said environment generates repetitive biochemical cycles that can favor, enhance or establish selective biological developments, thus favoring selective to certain species or the generation of certain biological products, and that can develop ecosystems that may or may not reach a permanent regime.
  • the generated ecosystems can be favored by any type of artificial action (that is, a cultivation action) of the control group of temperature, humidity, pH of aqueous suspension, concentration of nitrogen oxides, sulfur, iron and salts of sodium, potassium, calcium and magnesium, so that, as indicated, the development of a certain species or species is favored, as well as the generation of a specific biological product.
  • Figure 2 shows an example of practical application of the invention in an artificial forest, where a culture of suspended algae and nutrients (21) is permanently sprayed (22) in the form of an aerosol, generating a cloud (23) that absorbs solar radiation (24) and C0 2 (25) generating a high biomass yield, since the aerosol that precipitates (that is, sediments) is collected in the crop (21) and nebulized again.

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé de production de biomasse à travers l'utilisation d'aérosols aqueux qui contiennent des micro-organismes photosynthétiques. Ledit procédé comprend une première étape de production d'aérosol (1) constitué d'une suspension aqueuse de micro-organismes photosynthétiques; et d'une seconde étape de libération de l'aérosol dans un environnement d'air atmosphérique soumis au rayonnement solaire (3), de telle sorte que ladite suspension se maintienne pendant une durée suffisante pour que le rendement de production de biomasse soit positif, la suspension recevant le rayonnement solaire et étant en contact avec le CO2 atmosphérique, un procédé naturel de génération de biomasse étant ainsi obtenu.
PCT/ES2011/000082 2010-03-18 2011-03-18 Procédé de production de biomasse à travers l'utilisation d'aérosols aqueux qui contiennent des micro-organismes photosynthétiques WO2011113977A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP201000380 2010-03-18
ES201000380A ES2365312B1 (es) 2010-03-18 2010-03-18 Procedimiento de producción de biomasa mediante el uso de aerosoles acuosos que contienen microorganismos fotosintéticos.

Publications (1)

Publication Number Publication Date
WO2011113977A1 true WO2011113977A1 (fr) 2011-09-22

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Family Applications (1)

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PCT/ES2011/000082 WO2011113977A1 (fr) 2010-03-18 2011-03-18 Procédé de production de biomasse à travers l'utilisation d'aérosols aqueux qui contiennent des micro-organismes photosynthétiques

Country Status (2)

Country Link
ES (1) ES2365312B1 (fr)
WO (1) WO2011113977A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU501719A1 (ru) * 1974-04-10 1976-02-05 Республиканский Проектный Институт "Узгипросельстрой" Установка дл выращивани микроводорослей
SU1055435A1 (ru) * 1981-06-19 1983-11-23 Белорусский Научно-Исследовательский Проектно-Конструкторский Институт Рыбного Хозяйства Установка дл производства микроводорослей
US20080178739A1 (en) * 2006-07-10 2008-07-31 Greenfuel Technologies Corp. Photobioreactor systems and methods for treating CO2-enriched gas and producing biomass
WO2009134114A1 (fr) * 2008-04-30 2009-11-05 Tet Shin Ho Appareil de culture en masse de microalgues et procédé pour leur culture

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU501719A1 (ru) * 1974-04-10 1976-02-05 Республиканский Проектный Институт "Узгипросельстрой" Установка дл выращивани микроводорослей
SU1055435A1 (ru) * 1981-06-19 1983-11-23 Белорусский Научно-Исследовательский Проектно-Конструкторский Институт Рыбного Хозяйства Установка дл производства микроводорослей
US20080178739A1 (en) * 2006-07-10 2008-07-31 Greenfuel Technologies Corp. Photobioreactor systems and methods for treating CO2-enriched gas and producing biomass
WO2009134114A1 (fr) * 2008-04-30 2009-11-05 Tet Shin Ho Appareil de culture en masse de microalgues et procédé pour leur culture

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ZEBIB, T.: "Microalgae Grown in Photobioreactors for Mass Production of Biofuel", MICROALGAE GROWN IN PHOTOBIOREACTORS FOR MASS PRODUCTION OF BIOFUEL, 30 September 2008 (2008-09-30), RUTGERS UNIVERSITY. DEPARTMENT OF BIOENVIRONMENTAL ENGINEERING, Retrieved from the Internet <URL:http://www.water.rutgers.edu/Educational_Programs/Senior%20Design2008/Algae%20to%20Energy%20Report.pdf> [retrieved on 20110705] *

Also Published As

Publication number Publication date
ES2365312A1 (es) 2011-09-29
ES2365312B1 (es) 2012-04-20

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