WO2011108854A3 - 가지상 dna 복합체의 형성 촉진을 통한 핵산 검출 방법 - Google Patents

가지상 dna 복합체의 형성 촉진을 통한 핵산 검출 방법 Download PDF

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Publication number
WO2011108854A3
WO2011108854A3 PCT/KR2011/001452 KR2011001452W WO2011108854A3 WO 2011108854 A3 WO2011108854 A3 WO 2011108854A3 KR 2011001452 W KR2011001452 W KR 2011001452W WO 2011108854 A3 WO2011108854 A3 WO 2011108854A3
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WIPO (PCT)
Prior art keywords
branched dna
nucleic acids
complex formation
promoting
detecting nucleic
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PCT/KR2011/001452
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English (en)
French (fr)
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WO2011108854A2 (ko
Inventor
채치범
김경태
강종훈
Original Assignee
건국대학교 산학협력단
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Application filed by 건국대학교 산학협력단 filed Critical 건국대학교 산학협력단
Priority to JP2012556016A priority Critical patent/JP5681217B2/ja
Priority to EP11750911.7A priority patent/EP2543737B1/en
Priority to CN2011800123665A priority patent/CN102869787A/zh
Publication of WO2011108854A2 publication Critical patent/WO2011108854A2/ko
Publication of WO2011108854A3 publication Critical patent/WO2011108854A3/ko
Priority to US13/603,190 priority patent/US9868983B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/313—Branched oligonucleotides

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

가지상 DNA 복합체의 형성 촉진을 통한 핵산 검출 방법이 개시된다. 동일 반응 혼합물에서의 PCR, 가열변성 및 혼성화의 통합 수행에 의하여, 단일 사슬 올리고뉴클레오티드 프로브와 복수의 증폭된 표적 DNA 사이에서의 가지상(branched) DNA의 자기 조립을 촉진시킴으로써, 표적 핵산의 검출 신호 및 민감도를 극적으로 증가시킬 수 있다.
PCT/KR2011/001452 2010-03-04 2011-03-03 가지상 dna 복합체의 형성 촉진을 통한 핵산 검출 방법 WO2011108854A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2012556016A JP5681217B2 (ja) 2010-03-04 2011-03-03 分枝状ｄｎａ複合体の形成促進を通じた核酸検出方法
EP11750911.7A EP2543737B1 (en) 2010-03-04 2011-03-03 Method for detecting nucleic acids by promoting branched dna complex formation
CN2011800123665A CN102869787A (zh) 2010-03-04 2011-03-03 通过促进分枝状dna复合体的形成的核酸检测方法
US13/603,190 US9868983B2 (en) 2010-03-04 2012-09-04 Method for detecting nucleic acids by promoting branched DNA complex formation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20100019539 2010-03-04
KR10-2010-0019539 2010-03-04

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/603,190 Continuation US9868983B2 (en) 2010-03-04 2012-09-04 Method for detecting nucleic acids by promoting branched DNA complex formation

Publications (2)

Publication Number Publication Date
WO2011108854A2 WO2011108854A2 (ko) 2011-09-09
WO2011108854A3 true WO2011108854A3 (ko) 2012-01-12

Family

ID=44542715

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/001452 WO2011108854A2 (ko) 2010-03-04 2011-03-03 가지상 dna 복합체의 형성 촉진을 통한 핵산 검출 방법

Country Status (6)

Country Link
US (1) US9868983B2 (ko)
EP (1) EP2543737B1 (ko)
JP (1) JP5681217B2 (ko)
KR (1) KR101236695B1 (ko)
CN (1) CN102869787A (ko)
WO (1) WO2011108854A2 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105400776B (zh) * 2014-09-12 2019-12-31 深圳华大智造科技有限公司 寡核苷酸接头及其在构建核酸测序单链环状文库中的应用
CN105986324B (zh) * 2015-02-11 2018-08-14 深圳华大智造科技有限公司 环状小rna文库构建方法及其应用
JP6977978B2 (ja) * 2016-01-28 2021-12-08 株式会社Tba 標的核酸の検出方法
CN106047677B (zh) * 2016-05-19 2018-10-02 沈阳今唐基因与医学技术研究院 检测单细胞中核酸的微流控芯片及检测单细胞中核酸的方法

Citations (3)

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Publication number Priority date Publication date Assignee Title
US6465183B2 (en) * 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
US20080193934A1 (en) * 2006-07-17 2008-08-14 Brandeis University Specialized oligonucleotides and their use in nucleic acid amplification and detection
US20080305966A1 (en) * 2004-08-02 2008-12-11 Regis Peytavi Capture Probe Design for Efficient Hybridisation

Family Cites Families (8)

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US5849544A (en) * 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
EP0845047A2 (en) * 1995-08-14 1998-06-03 Abbott Laboratories All-in-one nucleic acid amplification assay
DE19730359A1 (de) * 1997-07-15 1999-01-21 Boehringer Mannheim Gmbh Integriertes Verfahren und System zur Amplifizierung und zum Nachweis von Nukleinsäuren
DE10253966B4 (de) * 2002-11-19 2005-03-24 Clondiag Chip Technologies Gmbh Microarray-basiertes Verfahren zur Amplifikation und Detektion von Nukleinsäuren in einem kontinuierlichen Prozess
KR100645259B1 (ko) 2004-08-26 2006-11-15 (주)차바이오메드 유형 특이적 인유두종바이러스 검출용 올리고뉴클레오티드프로브와 이를 이용한 인유두종바이러스 유전형 분석용ｄｎａ칩, 분석키트 및 그 제조 방법
US8841069B2 (en) 2005-12-29 2014-09-23 Korea Materials & Analysis Corporation Dendron-mediated DNA virus detection
EP2199409A3 (en) * 2005-12-29 2010-09-29 University-Industry Cooperation Foundation Konkuk University One step diagnosis by DNA chip
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Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465183B2 (en) * 1999-07-01 2002-10-15 Agilent Technologies, Inc. Multidentate arrays
US20080305966A1 (en) * 2004-08-02 2008-12-11 Regis Peytavi Capture Probe Design for Efficient Hybridisation
US20080193934A1 (en) * 2006-07-17 2008-08-14 Brandeis University Specialized oligonucleotides and their use in nucleic acid amplification and detection

Non-Patent Citations (3)

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Title
See also references of EP2543737A4 *
SHCHEPINOV, M. S. ET AL.: "Oligonucleotide dendrimers: synthesis and use as polylabelled DNA probes.", NUCLEIC ACIDS RESEARCH, vol. 25, no. 22, 15 November 1997 (1997-11-15), pages 4447 - 4454, XP002928476 *
WONG, D. M. ET AL.: "Branch capture reactions: displacers derived from asymmetric PCR.", NUCLEIC ACIDS RESEARCH, vol. 19, no. 9, 11 May 1999 (1999-05-11), pages 2251 - 2259, XP000204316 *

Also Published As

Publication number Publication date
US20130078629A1 (en) 2013-03-28
KR20110100585A (ko) 2011-09-14
WO2011108854A2 (ko) 2011-09-09
CN102869787A (zh) 2013-01-09
EP2543737A4 (en) 2013-10-23
JP5681217B2 (ja) 2015-03-04
KR101236695B1 (ko) 2013-02-25
EP2543737A2 (en) 2013-01-09
US9868983B2 (en) 2018-01-16
EP2543737B1 (en) 2017-08-02
JP2013520987A (ja) 2013-06-10

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