WO2011096775A2 - Appareil à deux étages pour insémination artificielle, apte à porter simultanément des cellules reproductrices de bovin et des substances favorisant la fertilisation et à injecter les cellules reproductrices et les substances favorisant la fertilisation dans un utérus en un seul acte - Google Patents

Appareil à deux étages pour insémination artificielle, apte à porter simultanément des cellules reproductrices de bovin et des substances favorisant la fertilisation et à injecter les cellules reproductrices et les substances favorisant la fertilisation dans un utérus en un seul acte Download PDF

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Publication number
WO2011096775A2
WO2011096775A2 PCT/KR2011/000800 KR2011000800W WO2011096775A2 WO 2011096775 A2 WO2011096775 A2 WO 2011096775A2 KR 2011000800 W KR2011000800 W KR 2011000800W WO 2011096775 A2 WO2011096775 A2 WO 2011096775A2
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WO
WIPO (PCT)
Prior art keywords
straw
artificial insemination
pregnancy
uterus
present
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Application number
PCT/KR2011/000800
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English (en)
Korean (ko)
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WO2011096775A3 (fr
Inventor
김소섭
Original Assignee
광개토한우농업법인 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority claimed from KR1020110010588A external-priority patent/KR101289383B1/ko
Application filed by 광개토한우농업법인 주식회사 filed Critical 광개토한우농업법인 주식회사
Publication of WO2011096775A2 publication Critical patent/WO2011096775A2/fr
Publication of WO2011096775A3 publication Critical patent/WO2011096775A3/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/21—Interferons [IFN]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase

Definitions

  • the present invention relates to a device capable of injecting a separate straw filled with a specific pregnancy-promoting substance and a straw filled with semen or fertilized egg simultaneously into one sheath tube and an artificial insemination device and injecting it into the genital organs of a cow by one procedure. More specifically, another straw containing certain pregnancy-promoting substances, such as IFN, IGF, MMP, TGF, IP-10, and sex hormones, is combined together in a sheath tube containing a ready-made semen or fertilized egg, and in one artificial insemination chamber.
  • the present invention relates to a method of increasing the convenience and pregnancy rate of a procedure by injecting two substances into the uterus of a cow by simultaneous operation in a single procedure.
  • Fertilization and fertilized egg transplants are a widespread and continuously expanding technology around the world for the improvement of cattle and other livestock. Nevertheless, due to various factors, the fertility rate due to artificial insemination is gradually decreasing. As a method for increasing pregnancy rate, improvements in specification management, disease control related to early absorption of fetuses and similar acids, and efficient estrus control techniques have been commercialized. On the other hand, attempts have been made to apply fertility enhancers to improve the activity of semen and eggs and fertilized eggs related to early fertilization and implantation.
  • TGF a major component in the suit, is added directly to the crystallization solution or injected into the genital organs before and after artificial insemination, and IFN-gamma and tau for promoting the implantation and suppressing the degeneration of the corpus luteum. Utilization, the use of MMPs are reported. In order to inject these substances into the uterus simultaneously with germ cells, the hazards, economics, usage, application and ease of treatment of germ cells should be considered first.
  • a straw containing an already produced semen or fertilized egg is inserted into an artificial insemination, and then injected or implanted into a cow's genital organs, and then the straw containing these substances is reinserted into an artificial insemination and additionally injected twice.
  • the hassle of infusion and consequent contamination of the uterus also increases.
  • This problem is limited to one-time injection of semen or fertilized eggs, and there is a limit to injecting germ cells into the uterus and further injecting gestation-promoting substances.
  • the present invention is to provide a method for encapsulating a pregnancy-promoting substance composed of a specific IFN, IGF, TGF, MMP, IP-10, sex hormone, etc. in artificial insemination or fertilized egg transplant.
  • the present invention is to provide a method of connecting a straw in which a germ cell is sealed with a specific pregnancy-promoting material.
  • the present invention is to provide a method of manufacturing a single-use artificial insemination that can be equipped with a ready-made encapsulated germ cell and the product developed by the method at the same time.
  • another object of the present invention is to provide a method of injecting the product encapsulated by the above method and a ready-made semen or fertilized egg at the same time and then simultaneously injected into the uterus by one procedure.
  • the present inventors secreted by IGF, TGF, endometrium and trophoblast cells, IFN having angiogenesis and luteal degeneration suppression function during implantation, or MMP, IP-10, sex hormone, etc. Is known to act to increase implantation and pregnancy. These materials were treated or combined in appropriate concentrations and stored in 0.25 or 0.50 ml of straw, and then injected into the uterus at the same time as semen or fertilized eggs to develop implantation and pregnancy rates.
  • the two-stage artificial insemination of the present invention is equipped with a germ cell-filled straw and pregnancy-promoting material-filled straw at the same time in one sheath tube and the artificial insemination by injecting them into the cow's genitals by one simultaneous operation and the simplicity of the procedure The pregnancy rate could be dramatically improved.
  • FIG. 1 is a perspective structural diagram illustrating the entire two-stage artificial insemination device according to the present invention.
  • FIG. 2 is a longitudinal cross-sectional view illustrating the internal structure of the two-stage artificial crystal of FIG. 1.
  • FIG 3 is an illustration of an example coupled by a connecting straw of the present invention.
  • FIG. 4 is a schematic view of the movable pressure plate and the rail-type movable part of the present invention.
  • FIG. 5 is a production diagram of a two-stage artificial insemination of the present invention.
  • Pregnancy promoting substance 50 of the present invention includes IFN, TGF, MMP, IGF, IP-10, sex hormones.
  • the substance may be, but is not limited to, a substance in semen, a recombinant protein thereof or trophoblastic vesicles cultured in vitro.
  • these materials may be enclosed mixed with germ cells in the same first straw 10, or each compartment may be distinctly enclosed.
  • these substances may be composed of a single substance only in each of the first or second straws 10 and 20 independent of each other, may be separately enclosed in each independent compartment in the same straw, or mixed in a predetermined ratio, It may be dissolved or combined and enclosed in the same compartment.
  • the material of the coupling tube 25 for the connection of the first and second straws 10 and 20 is silicone or the same polyethylene resin as that of the 0.50ml and 0.25ml standard artificial artificial insemination (fertilization egg). As such, it can be produced in a length of 0.5 ⁇ 5cm.
  • the inner diameter of the coupling portion should be firmly coupled to the outer diameter of the commercial straw and there should be no leakage, and should be made narrower than the inner diameter of the sheath pipe 110 to be moved within the sheath pipe 110.
  • connection portion where the second straw 20 of the ready-made semen and the second straw 20 in which the material is enclosed by the coupling pipe 25 should be coupled so as not to be separated from the rear by the appearance.
  • the close contact with the major part of the sheath pipe 110, there was no leakage of semen, fertilized egg and pregnancy-promoting material (50) when pushing the artificial insemination (120, propeller), and the surface sealing plug (30) , See FIG. 2) is also produced so as not to occur.
  • Components constituting the two-stage artificial insemination according to the present invention may be made of a plated material of two or three tubular or linear stainless steel or brass casting. These are external parts for mounting the first and second straws 20 and the sheath pipe 110, the sheath pipe fixing part 113 and the sheath pipe fixing part 113 where the sheath pipe 110 is fixed so as not to be separated.
  • the appearance of the present invention is a front end leading to the genital organs, the sieve part covered with the sheath tube 110, the fixing portion 113 for fixing the sheath tube 110 is not detached, a fixed type for the operation and propulsion of artificial insemination. It consists of a pressure plate 130 or a movable pressure plate 140.
  • the outermost end of the sheath pipe 110 is configured to have an inclined portion 111 of an inclined form in which the outer diameter is narrowed, so as to secure easy insertion degree according to the detachment and the stretchability of the first straw 10. .
  • the outer appearance is a tubular shape of the length 425mm, outer diameter 3.8mm, inner diameter 3.2mm, can be shortened by one straw length compared to the preformed appearance, can be equipped with a commercially modified quartz sheath tube 110,
  • the inclination part 111 of the front of the sheath pipe 110 prevents the second and first straws 20 and 10 from coming off, and the sheath pipe fixing part 113 and the pressure plate are attached to the rear part thereof.
  • the inner tube may be attached.
  • the sheath tube fixing part 113 located in the rear portion of the exterior is processed into a spiral protrusion of 4 to 5 times larger than the exterior dimension to rotate or fix the sheath tube 110 to fix the spiral or sheath tube 110.
  • the pressure of the spring 41 acts on the canine clamp (40, see FIG. 2) and can be manufactured in a canine type that prevents the departure of the sheath tube 110, which is screwed, caulked or bonded to the exterior. It is firmly attached via the medium.
  • the fixed or movable pressure plate (130, 140) of the artificial clarifier is easy to grip, the disk shape of 15mm diameter, 3mm thickness for generating the pressure of the inner core (120, propeller) firmly in the rear of the exterior Attached.
  • the inner tube 115 is 190mm, outer diameter 3.2mm, inner diameter 2.4mm shortened to 1 to 2 times the length of the normal straw in the direction from the front to the rear portion, it is inserted into the interior of the exterior and firmly attached , Straw mounted on the sheath pipe 110 as an appearance is prevented from escaping to the exterior rear portion, and guided the inner core for propulsion (120, propeller) was easy to move forward and backward.
  • the propulsion inner core (120, propeller) may be a single wire or composite wire of length 453mm, thickness 2.4mm, 1 to 2 fixed and movable pressure plate (130 or 140) is attached to the rear portion, in the case of a single wire
  • the latter half can also be manufactured as a hinge.
  • the propulsion inner core (120, propeller) is processed by forming a cross-section half moon shape or one side half moon shape, quadrilateral with the cross-section in the length of about 5 ⁇ 15cm in the rear portion of the propeller to operate by operating the movable pressure plate 140 Can be made.
  • the pressure plate of the inner core 120 may be manufactured in a disc shape having a thickness of 3 mm and a diameter of 15 mm, one of which is fixed to the second half of the propelling inner core (120, propeller) and one may be movable.
  • the movable pressure plate 140 is mounted on the processed inner core and manufactured to move forward and backward.
  • both ends of the first straw 10 in which the germ cells are enclosed and the fertility enhancer 50 are enclosed are cut and coupled to the binding portion, and then the germ cells are adjusted to be located at the tip of the artificial insemination. Inserted into the sheath tube 110 is mounted to the artificial insemination of the present invention.
  • the flexibility of the front end is improved, and the exterior of the artificial insemination unit is connected to the first and second straws 20.
  • the separation and leakage of the two straws combined are prevented.
  • the inner core (propulsion) propulsion method of the artificial insemination presses the external pressure plate and the movable inner core (120, propeller) pressure plate at the same time, and then by pressing the fixed inner core pressure plate in two stages, the germ cells in the front straw First, it can be injected into the uterus and the pregnancy promoting material 50 in the rear straw can be injected.
  • the two-stage artificial insemination machine is superior in flexibility in the front part of the sheath tube 110 coupled to the artificial insemination, and the use of the sheath tube 110 and the number of injections are reduced by one half compared to the conventional artificial insemination. It was effective in injecting germ cells and gestation enhancers (50) at the same time.
  • One injection group One-time procedure that combines semen straw and IFN straw, and is mounted on a two-stage artificial insemination
  • Twice injection group Twice the procedure of injecting semen into a ready-made intraocular lens and injecting IFN into a ready-made intraocular lens secondly.
  • the co-transplantation method of inteferon using the two-stage artificial insemination of the present invention has a higher pregnancy-promoting effect and can also reduce the consumption of cistern tube 110 compared to the additional implantation method of interferon using the preformed artificial insemination. It can be seen.
  • the pregnancy rate was significantly higher in the group implanted with the fertilized egg and interferon than the control group transplanted with the fertilized egg alone.
  • connection device that can be connected to the straw filled with the pregnancy-promoting substance (50) at the same time to combine two straws in one sheath tube 110 It was confirmed that there was no leak in the site, the insemination of the intraocular lens (120, propeller) was easily propelled, and these substances were simultaneously injected into the uterus.
  • connection straw of the present invention is made of polyethylene or silicone material, and the inner diameters of both ends thereof may be firmly combined with the outer diameter of the straw to be bonded, and may be elastic, and pass through the surface sealing and the inner core (120, propeller). Withstands rupture, germ cells and gestational promoter 50 do not leak, the length of the coupling portion may be within 1 ⁇ 5cm.
  • the inner tube of the artificial insemination of the present invention has a hook portion 1/2 to the length of the conventional artificial insemination in order to prevent the straw is attached to one artificial insemination with two straws connected to the rear side to prevent the departure It may be manufactured to be shortened or extended twice, or may be manufactured to be adjustable in length.
  • the outer surface of the artificial insemination of the present invention by one length of the straw compared to the preformed appearance so that the straw can be firmly mounted in close contact with the straw in a state in which two straws are connected to one artificial insemination It can be manufactured to be shortened, it can be manufactured to finely adjust the coupling length by the helical projection of the fixing portion.
  • the front end portion of the sheath tube 110 in the form after combining the appearance of the artificial insemination of the present invention and the sheath tube 110 is free from the outside as much as the length of the straw is sheath tube 110 of the non-sensitized portion Extinction can be significantly improved.
  • the inner core for the surface sealing propulsion (120, propeller) of the artificial insemination of the present invention may be produced in a single wire or a composite wire of stainless material so as to extend by one length.
  • the longer part of the piston may be made to be bent so as not to be inconvenient during operation, and the bent portion of the single wire may be manufactured to be hinged.
  • the propulsion inner core of the present invention can be manufactured to act as a rail of the movable pressure plate by processing the cross section of the portion corresponding to the length of one straw in the rear side semicircle, one side semicircle, square.
  • the pressure plate for the propulsion of the inner core (120, propeller) of the present invention may be two, one is fixed, the other is not fixed, the movable can be moved back and forth on the processed inner core without being fixed Can be made into mold.
  • the method for mounting two straws in the artificial insemination of the present invention is to cut one end of the straw filled with the pregnancy-promoting substance 50, and to cut after connecting one end of the straw filled with germ cells
  • an apparatus which are coupled to the sheath tube 110 such that the germ-filled straw is located at the tip of the injector, and finally mounted in the artificial insemination of the present invention.
  • the piston propulsion method of the artificial insemination of the present invention by first compressing the pressure plate and the movable pressure plate mounted on the body part, the germ cells are first injected into the uterus, and then the pressure plate attached to the body part and the fixed pressure plate of the inner core 2 By compressing differentially, the pregnancy promoting substance 50 may be injected.
  • the tip portion of the sheath tube 110 of the present invention is improved in flexibility, it is excellent in adaptability to the degree of flexion of the uterus at the time of intrauterine insertion, and is easy to be injected into the core, thereby causing less endometrial wounds. .
  • the two-stage artificial insemination of the present invention was able to dramatically improve the convenience and pregnancy rate of the procedure by co-insertion and co-injection of bovine germ cells and gestation promoting material 50 into the same artificial insemination. .

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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Abstract

La présente invention porte sur le développement d'équipement pour l'injection simultanée de cellules reproductrices et de substances spécifiques destinées à favoriser la fertilisation et la capacité de reproduction, dans un organe génital d'une vache. Plus particulièrement, la présente invention porte sur un appareil d'insémination artificielle et sur une technique utilisant l'appareil, l'appareil d'insémination artificielle étant équipé à la fois d'une paillette encapsulée avec un sperme ou un embryon et d'une autre paillette encapsulée avec des substances favorisant la fertilisation pour favoriser la fertilisation ou induire une implantation dans l'utérus de la vache durant l'insémination artificielle ou le transfert d'embryon, et ainsi, l'appareil d'insémination artificielle peut réaliser une injection de sperme ou un transfert d'embryon simultanément en un seul acte. L'appareil de la présente invention peut injecter simultanément des cellules reproductrices et des substances favorisant la fertilisation dans l'utérus d'une vache en un seul acte, permettant ainsi d'améliorer significativement la commodité d'utilisation et le taux de grossesse.
PCT/KR2011/000800 2010-02-08 2011-02-08 Appareil à deux étages pour insémination artificielle, apte à porter simultanément des cellules reproductrices de bovin et des substances favorisant la fertilisation et à injecter les cellules reproductrices et les substances favorisant la fertilisation dans un utérus en un seul acte WO2011096775A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20100011422 2010-02-08
KR10-2010-0011422 2010-02-08
KR1020110010588A KR101289383B1 (ko) 2010-02-08 2011-02-07 소 생식세포와 임신증진물질을 동시 장착 후 1회로 자궁내에 주입이 가능한 2단 인공수정기
KR10-2011-0010588 2011-02-07

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WO2011096775A2 true WO2011096775A2 (fr) 2011-08-11
WO2011096775A3 WO2011096775A3 (fr) 2012-01-05

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3277257A4 (fr) * 2015-04-03 2018-11-21 Nanocell Ltd. Compositions de remodelage d'une matrice extracellulaire et leurs procédés d'utilisation
CN109363799A (zh) * 2018-10-30 2019-02-22 贵州牧林农业科技发展有限公司 一种马牛用输精器

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990041449U (ko) * 1998-05-19 1999-12-15 김강권 가축용 수정란 이식장치
KR20040097726A (ko) * 2003-05-13 2004-11-18 배민기 가축용 수정란 이식장치
KR20050024030A (ko) * 2003-09-04 2005-03-10 주식회사 에이비에스인코리아 수정란 이식용 스트로우
KR20090123340A (ko) * 2008-05-27 2009-12-02 재단법인서울대학교산학협력재단 스트로우를 이용한 포유동물의 수정란 이식 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990041449U (ko) * 1998-05-19 1999-12-15 김강권 가축용 수정란 이식장치
KR20040097726A (ko) * 2003-05-13 2004-11-18 배민기 가축용 수정란 이식장치
KR20050024030A (ko) * 2003-09-04 2005-03-10 주식회사 에이비에스인코리아 수정란 이식용 스트로우
KR20090123340A (ko) * 2008-05-27 2009-12-02 재단법인서울대학교산학협력재단 스트로우를 이용한 포유동물의 수정란 이식 방법

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3277257A4 (fr) * 2015-04-03 2018-11-21 Nanocell Ltd. Compositions de remodelage d'une matrice extracellulaire et leurs procédés d'utilisation
US10722560B2 (en) 2015-04-03 2020-07-28 Nanocell Ltd. Compositions for remodeling extracellular matrix and methods of use thereof
US11382959B2 (en) 2015-04-03 2022-07-12 Nanocell Ltd. Compositions for remodeling extracellular matrix and methods of use thereof
US11779636B2 (en) 2015-04-03 2023-10-10 Nanocell Ltd. Compositions for remodeling extracellular matrix and methods of use thereof
CN109363799A (zh) * 2018-10-30 2019-02-22 贵州牧林农业科技发展有限公司 一种马牛用输精器

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