WO2011071358A3 - Proceso para la producción de l-lactato mediante fermentación de lactosuero con lactococcus lactis subespecie lactis - Google Patents
Proceso para la producción de l-lactato mediante fermentación de lactosuero con lactococcus lactis subespecie lactis Download PDFInfo
- Publication number
- WO2011071358A3 WO2011071358A3 PCT/MX2010/000143 MX2010000143W WO2011071358A3 WO 2011071358 A3 WO2011071358 A3 WO 2011071358A3 MX 2010000143 W MX2010000143 W MX 2010000143W WO 2011071358 A3 WO2011071358 A3 WO 2011071358A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- lactate
- culture medium
- fermentation
- production
- lactis
- Prior art date
Links
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 title abstract 7
- 229940116871 l-lactate Drugs 0.000 title abstract 5
- 241000194035 Lactococcus lactis Species 0.000 title abstract 4
- 235000014897 Streptococcus lactis Nutrition 0.000 title abstract 4
- 238000000855 fermentation Methods 0.000 title abstract 4
- 230000004151 fermentation Effects 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 239000005862 Whey Substances 0.000 title abstract 2
- 102000007544 Whey Proteins Human genes 0.000 title abstract 2
- 108010046377 Whey Proteins Proteins 0.000 title abstract 2
- 239000001963 growth medium Substances 0.000 abstract 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 abstract 2
- 238000009825 accumulation Methods 0.000 abstract 2
- 239000006227 byproduct Substances 0.000 abstract 2
- 239000008101 lactose Substances 0.000 abstract 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 229930182830 galactose Natural products 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 150000002772 monosaccharides Chemical class 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 230000037361 pathway Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/56—Lactic acid
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
- A23J3/08—Dairy proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/231—Lactis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
En la presente invención se describe un proceso para producir L-lactato mediante la fermentación de lactosuero con Lactococcus lactis subespecie lactis. Dicho proceso contempla la etapa de inocular (a) el medio de cultivo con Lactococcus lactis subespecie lactis, la etapa de producir y acumular (b) el L-lactato en el medio de cultivo y la etapa de extraer (c) determinado volumen de caldo fermentado del reactor biológico. La fermentación en el medio de cultivo se lleva a cabo a temperatura constante en el rango comprendido entre 22 a 29°C, bajo estrictas condiciones anaerobias y a un pH en el rango comprendido entre 5 a 6. Estas condiciones de operación encaminan la ruta homofermentativa de Lactococcus lactis subespecie lactis, evitando así la generación de subproductos diferentes al L-Lactato, y permitir el uso integral de la lactosa, evitando la acumulación de glucosa y galactosa en el medio de cultivo fermentado. Finalmente se recupera el medio de cultivo fermentado del recipiente de fermentación con una concentración de L-Lactato de al menos 20g/l con una pureza óptica mayor al 90%, y con una concentración de no más de 0.3g/l de subproductos diferentes al L-Ácido láctico y/o de alguno de los monosacáridos que constituyen la lactosa.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MXMX/A/2009/013589 | 2009-12-11 | ||
| MX2009013589A MX341691B (es) | 2009-12-11 | 2009-12-11 | Proceso para la produccion de l-lactato mediante fermentacion de lactosuero con lactococcus lactis subespecie lactis. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2011071358A2 WO2011071358A2 (es) | 2011-06-16 |
| WO2011071358A3 true WO2011071358A3 (es) | 2011-11-24 |
Family
ID=44146084
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/MX2010/000143 WO2011071358A2 (es) | 2009-12-11 | 2010-12-03 | Proceso para la producción de l-lactato mediante fermentación de lactosuero con lactococcus lactis subespecie lactis |
Country Status (2)
| Country | Link |
|---|---|
| MX (1) | MX341691B (es) |
| WO (1) | WO2011071358A2 (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115124414A (zh) * | 2022-05-20 | 2022-09-30 | 山东省食品发酵工业研究设计院 | 一种高光学纯度3-羟基丁酮的制备方法及其应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1496108A1 (en) * | 2002-03-26 | 2005-01-12 | New Century Fermentation Research Ltd. | Method of continuous culture of anaerobic bacterium |
| US7537925B2 (en) * | 2001-10-04 | 2009-05-26 | Forskarptent I Syd Ab | Mutated Lactococcus strain |
-
2009
- 2009-12-11 MX MX2009013589A patent/MX341691B/es active IP Right Grant
-
2010
- 2010-12-03 WO PCT/MX2010/000143 patent/WO2011071358A2/es active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7537925B2 (en) * | 2001-10-04 | 2009-05-26 | Forskarptent I Syd Ab | Mutated Lactococcus strain |
| EP1496108A1 (en) * | 2002-03-26 | 2005-01-12 | New Century Fermentation Research Ltd. | Method of continuous culture of anaerobic bacterium |
Non-Patent Citations (2)
| Title |
|---|
| HOFVENDAHL, K. ET AL.: "B. Factors affecting the fermentative lactic acid production from renewable resources.", ENZYME AND MICROBIAL TECHNOLOGY., vol. 26, no. 2, February 2000 (2000-02-01), pages 87 - 107, XP002500788, DOI: doi:10.1016/S0141-0229(99)00155-6 * |
| ROUKAS, T. ET AL.: "Production of lactic acid from deproteinized whey by coimmobilized Lactobacillus casei and Lactococcus lactis cells.", ENZYME AND MICROBIAL TECHNOLOGY., vol. 13, no. 1, January 1991 (1991-01-01), pages 33 - 38, XP023854200, DOI: doi:10.1016/0141-0229(91)90185-D * |
Also Published As
| Publication number | Publication date |
|---|---|
| MX341691B (es) | 2016-07-05 |
| MX2009013589A (es) | 2011-06-15 |
| WO2011071358A2 (es) | 2011-06-16 |
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