WO2011065753A3 - 형광 강도의 조절을 이용한 유세포 분석법 - Google Patents

형광 강도의 조절을 이용한 유세포 분석법 Download PDF

Info

Publication number
WO2011065753A3
WO2011065753A3 PCT/KR2010/008366 KR2010008366W WO2011065753A3 WO 2011065753 A3 WO2011065753 A3 WO 2011065753A3 KR 2010008366 W KR2010008366 W KR 2010008366W WO 2011065753 A3 WO2011065753 A3 WO 2011065753A3
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
flow cytometry
conjugated
cell populations
color
Prior art date
Application number
PCT/KR2010/008366
Other languages
English (en)
French (fr)
Other versions
WO2011065753A2 (ko
Inventor
한경자
Original Assignee
가톨릭대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 가톨릭대학교 산학협력단 filed Critical 가톨릭대학교 산학협력단
Priority to US13/510,254 priority Critical patent/US20120231473A1/en
Priority claimed from KR1020100117617A external-priority patent/KR101251590B1/ko
Publication of WO2011065753A2 publication Critical patent/WO2011065753A2/ko
Publication of WO2011065753A3 publication Critical patent/WO2011065753A3/ko

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

본 발명은 동일 색상의 형광 색소가 컨쥬게이트 되어 있는 항체에 의해 타겟팅되는 세포집단들이, 항체의 종류에 따라 상이한 형광 강도를 나타내도록 조절하는 것을 포함하는 새로운 유세포 분석 방법을 제공한다. 하나의 색상 당 하나의 항체를 이용함으로써 하나의 타겟의 양성 및 음성만을 분류할 수 있었던 종래의 유세포 분석법과는 달리, 본 발명의 방법에서는 단일 색상의 형광 색소가 컨쥬게이트되어 있는 여러 종류의 항체가 각기 상이한 형광 강도를 나타내도록 조절하거나, 항체의 종류에 따라 형광색소가 컨쥬게이트되어 있는 항체의 양을 상이하게 조절함으로써, 하나의 색상으로도 다수개의 타겟의 양성 및 음성을 분류할 수 있게 되므로, 한정된 색상을 분류해 낼 수 있는 현재의 유세포 분석기를 이용하더라도 임상적으로 확인이 필요한 다양한 세포 집단을 분류해 낼 수 있게 된다.
PCT/KR2010/008366 2009-11-24 2010-11-24 형광 강도의 조절을 이용한 유세포 분석법 WO2011065753A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/510,254 US20120231473A1 (en) 2009-11-24 2010-11-24 Flow cytometry method through the control of fluorescence intensities

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20090113899 2009-11-24
KR10-2009-0113899 2009-11-24
KR1020100117617A KR101251590B1 (ko) 2009-11-24 2010-11-24 형광 강도의 조절을 이용한 유세포 분석법
KR10-2010-0117617 2010-11-24

Publications (2)

Publication Number Publication Date
WO2011065753A2 WO2011065753A2 (ko) 2011-06-03
WO2011065753A3 true WO2011065753A3 (ko) 2011-11-17

Family

ID=44067102

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/008366 WO2011065753A2 (ko) 2009-11-24 2010-11-24 형광 강도의 조절을 이용한 유세포 분석법

Country Status (1)

Country Link
WO (1) WO2011065753A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109298174A (zh) * 2018-09-26 2019-02-01 姜云瀚 一种多色免疫荧光标记方法和成像方法
CN114216836A (zh) * 2021-12-02 2022-03-22 星汉德生物医药(大连)有限公司 一种特异性检测产品中tcr阳性t细胞比例的流式方法
CN115290875A (zh) * 2022-08-15 2022-11-04 无锡市人民医院 一种6色tbnk淋巴细胞亚群检测试剂盒和检测方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05107249A (ja) * 1991-02-04 1993-04-27 Toyobo Co Ltd リガンド・レセプター反応の高感度検出法
JP2001518624A (ja) * 1997-09-26 2001-10-16 ユニバーシティ・オブ・ワシントン 同時の粒子分離および化学反応
JP2005537781A (ja) * 2002-02-14 2005-12-15 イムニベスト・コーポレイション 低コストで細胞計数するための方法およびアルゴリズム
WO2007097377A1 (ja) * 2006-02-24 2007-08-30 The Furukawa Electric Co., Ltd. フローサイトメトリーによる生体分子の定量システム、その定量方法、細胞の検出・分取システム、その検出・分取方法、それらに用いる蛍光シリカ粒子、及び複数個の蛍光シリカ粒子を組み合わせたキット
KR20070102177A (ko) * 2006-04-14 2007-10-18 주식회사 디지탈바이오테크놀러지 세포표면 표지의 검출 및 계수 방법

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05107249A (ja) * 1991-02-04 1993-04-27 Toyobo Co Ltd リガンド・レセプター反応の高感度検出法
JP2001518624A (ja) * 1997-09-26 2001-10-16 ユニバーシティ・オブ・ワシントン 同時の粒子分離および化学反応
JP2005537781A (ja) * 2002-02-14 2005-12-15 イムニベスト・コーポレイション 低コストで細胞計数するための方法およびアルゴリズム
WO2007097377A1 (ja) * 2006-02-24 2007-08-30 The Furukawa Electric Co., Ltd. フローサイトメトリーによる生体分子の定量システム、その定量方法、細胞の検出・分取システム、その検出・分取方法、それらに用いる蛍光シリカ粒子、及び複数個の蛍光シリカ粒子を組み合わせたキット
JP2009014729A (ja) * 2006-02-24 2009-01-22 Furukawa Electric Co Ltd:The フローサイトメトリーによる細胞の検出・分取システム、及び検出・分取方法
KR20070102177A (ko) * 2006-04-14 2007-10-18 주식회사 디지탈바이오테크놀러지 세포표면 표지의 검출 및 계수 방법

Also Published As

Publication number Publication date
WO2011065753A2 (ko) 2011-06-03

Similar Documents

Publication Publication Date Title
Hewitt et al. Phenotypic heterogeneity in fungi: importance and methodology
WO2011065753A3 (ko) 형광 강도의 조절을 이용한 유세포 분석법
WO2015181343A3 (en) Multiplex assay for improved scoring of tumor tissues stained for pd-l1
Cabanelas et al. Sorting cells of the microalga Chlorococcum littorale with increased triacylglycerol productivity
WO2017040813A3 (en) Detection of gene loci with crispr arrayed repeats and/or polychromatic single guide ribonucleic acids
EP2100970A3 (en) Ig genes specific oligonucleotides and uses thereof
ES2527592T3 (es) Métodos basados en microARN para el diagnóstico del cáncer de colon
Park et al. Mechanistic elements and critical factors of cellular reprogramming revealed by stepwise global gene expression analyses
WO2016091888A3 (en) Methods, kits and compositions for phenotyping pancreatic ductal adenocarcinoma behaviour by transcriptomics
WO2011014740A3 (en) Methods and compositions for identifying and validating modulators of cell fate
US20100087006A1 (en) Use of fluorescent protein in cyanobacteria and algae for improving photosynthesis and preventing cell damage
WO2020168279A3 (en) Artificial expression constructs for selectively modulating gene expression in selected neuronal cell populations
CN104449661A (zh) 基于dna调控的量子点的新型信息处理方法
Cantrell et al. A mutant of Chlamydomonas without LHCSR maintains high rates of photosynthesis, but has reduced cell division rates in sinusoidal light conditions
WO2009102748A3 (en) Alternatively transcribed genes associated with schizophrenia
Schwartzman et al. Bacterial growth in multicellular aggregates leads to the emergence of complex life cycles
WO2012056451A3 (en) Peripheral blood gene markers for early diagnosis of parkinson's disease
Kim et al. A mechanogenetic role for the actomyosin complex in branching morphogenesis of epithelial organs
EP2211174A3 (en) Multicolored particles
Ma et al. Applications of single-cell technology on bacterial analysis
Worley et al. Time-and dose-dependent effects of ethanol on mouse embryonic stem cells
WO2010030167A3 (en) Method of detection and diagnosis of oral and nasopharyngeal cancers
CN103667521B (zh) 一组用于检测虹彩病毒的引物及检测方法
WO2008137629A3 (en) Identifying germline competent embryonic stem cells
CN103865861A (zh) 基于LuxS缺陷的变异链球菌AMC代谢恢复株

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10833565

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 13510254

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 10833565

Country of ref document: EP

Kind code of ref document: A2