WO2011059920A3 - Procédés de génération de cellules souches neuronales - Google Patents

Procédés de génération de cellules souches neuronales Download PDF

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Publication number
WO2011059920A3
WO2011059920A3 PCT/US2010/055836 US2010055836W WO2011059920A3 WO 2011059920 A3 WO2011059920 A3 WO 2011059920A3 US 2010055836 W US2010055836 W US 2010055836W WO 2011059920 A3 WO2011059920 A3 WO 2011059920A3
Authority
WO
WIPO (PCT)
Prior art keywords
neural stem
stem cells
subject
methods
present disclosure
Prior art date
Application number
PCT/US2010/055836
Other languages
English (en)
Other versions
WO2011059920A2 (fr
Inventor
Yadong Huang
Karen Lee Ring
Original Assignee
The J. David Gladstone Institutes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The J. David Gladstone Institutes filed Critical The J. David Gladstone Institutes
Priority to EP10830566.5A priority Critical patent/EP2499241B8/fr
Publication of WO2011059920A2 publication Critical patent/WO2011059920A2/fr
Publication of WO2011059920A3 publication Critical patent/WO2011059920A3/fr
Priority to US13/467,475 priority patent/US8815589B2/en
Priority to US14/335,246 priority patent/US9249391B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Abstract

La présente invention concerne des procédés de génération de cellules souches neuronales à partir de cellules somatiques différenciées. La présente invention concerne également des cellules souches neuronales induites générées par un des procédés précités, ainsi que des cellules différenciées générées à partir d'une cellule souche neuronale induite précitée. Une cellule souche neuronale précitée, ainsi que des cellules différenciées dérivées d'une cellule souche neuronale précitée, sont utilisées dans diverses aux applications qui sont également décrites dans la présente invention.
PCT/US2010/055836 2009-11-10 2010-11-08 Procédés de génération de cellules souches neuronales WO2011059920A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP10830566.5A EP2499241B8 (fr) 2009-11-10 2010-11-08 Procédés de génération de cellules souches neuronales
US13/467,475 US8815589B2 (en) 2009-11-10 2012-05-09 Methods of generating neural stem cells
US14/335,246 US9249391B2 (en) 2009-11-10 2014-07-18 Methods of generating neural stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25988509P 2009-11-10 2009-11-10
US61/259,885 2009-11-10

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/467,475 Continuation-In-Part US8815589B2 (en) 2009-11-10 2012-05-09 Methods of generating neural stem cells

Publications (2)

Publication Number Publication Date
WO2011059920A2 WO2011059920A2 (fr) 2011-05-19
WO2011059920A3 true WO2011059920A3 (fr) 2011-07-28

Family

ID=43992350

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/055836 WO2011059920A2 (fr) 2009-11-10 2010-11-08 Procédés de génération de cellules souches neuronales

Country Status (3)

Country Link
US (2) US8815589B2 (fr)
EP (1) EP2499241B8 (fr)
WO (1) WO2011059920A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2499241B8 (fr) 2009-11-10 2018-02-21 The J. David Gladstone Institutes Procédés de génération de cellules souches neuronales
WO2012164137A1 (fr) 2011-05-30 2012-12-06 Fundación Investigación En Regeneración Del Sistema Nervioso Cellules mères et cellules de la souche neurale dérivées de la glie olfactive enveloppante, et leurs applications
CN102827812B (zh) * 2011-06-17 2014-12-24 中国科学院动物研究所 诱导型神经干细胞的制备方法及应用
GB201112433D0 (en) * 2011-07-20 2011-08-31 Univ Leuven Kath Novel method for generation of neural progenitor cells
WO2017117229A1 (fr) 2015-12-30 2017-07-06 Wake Forest University Health Sciences Complexes intestin-sphincter d'ingénierie tissulaire et leurs procédés de fabrication
US11311367B2 (en) 2012-01-31 2022-04-26 Wake Forest University Health Sciences Tissue-engineered gut-sphincter complexes and methods of making the same
WO2013124309A1 (fr) * 2012-02-20 2013-08-29 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Reprogrammation directe de cellules somatiques en cellules souches neurales
US20150087594A1 (en) * 2012-03-21 2015-03-26 Merck Patent Gmbh Induced neural stem cells
PL223189B1 (pl) * 2012-11-15 2016-10-31 Celther Polska Spółka Z Ograniczoną Odpowiedzialnością Komórka iNS oraz sposób reprogramowania komórek somatycznych za pomocą czynnika Sox2 oraz Sox2 i c-Myc do komórki iNS
EP2970877B1 (fr) * 2013-03-15 2018-07-25 Wake Forest University Health Sciences Différenciation de cellules progénitrices neurales
US20140271584A1 (en) * 2013-03-15 2014-09-18 The Research Foundation For The State University Of New York Methods and Compositions for Direct Reprogramming of Somatic Cells to Stem Cells, and Uses of these Cells
KR20140121317A (ko) * 2013-04-06 2014-10-15 서울대학교산학협력단 비신경 세포로부터 유도 신경 줄기 세포의 생산 방법 및 이에 의하여 생산되는 유도 신경 줄기 세포
US20140371304A1 (en) * 2013-06-13 2014-12-18 Sucampo Ag Method for suppressing tumorigenicity of stem cells
EP3277329A4 (fr) 2015-03-31 2018-12-05 The University of North Carolina at Chapel Hill Véhicules d'apport pour cellules souches et leurs utilisations
US10286009B2 (en) * 2015-05-16 2019-05-14 Asterias Biotherapeutics, Inc. Pluripotent stem cell-derived oligodendrocyte progenitor cells for the treatment of spinal cord injury
JP2019509744A (ja) 2016-03-30 2019-04-11 アステリアス バイオセラピューティクス インコーポレイテッド オリゴデンドロサイト前駆細胞組成物
CN113474451A (zh) 2019-01-23 2021-10-01 阿斯特里亚斯生物疗法股份有限公司 来自人多能干细胞的背源性少突胶质祖细胞

Citations (4)

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US20050032207A1 (en) * 2001-09-12 2005-02-10 Anna Wobus Method for isolating, culturing and differentiating intestinal stem cells for therapeutic use
US7314721B2 (en) * 2000-01-21 2008-01-01 Ludwig Institute For Cancer Research Small cell lung cancer associated antigens and uses therefor
US20080260829A1 (en) * 2004-10-27 2008-10-23 New York Society For The Ruptured And Crippled Maintaining The Hospital For Special Surgery Bmp Gene and Fusion Protein
US20090191159A1 (en) * 2007-06-15 2009-07-30 Kazuhiro Sakurada Multipotent/pluripotent cells and methods

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2751345B1 (fr) 1996-07-16 1998-09-18 Univ Paris Curie Lignees d'encapsidation hautement productrices
US20020009743A1 (en) 2000-05-17 2002-01-24 Carpenter Melissa K. Neural progenitor cell populations
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US8338176B2 (en) 2007-07-30 2012-12-25 The Board Of Trustees Of The Leland Stanford Junior University Derivation of neural stem cells from embryonic stem cells
US9005966B2 (en) * 2007-11-19 2015-04-14 The Regents Of The University Of California Generation of pluripotent cells from fibroblasts
AU2008338989A1 (en) * 2007-12-17 2009-06-25 Gliamed, Inc. Stem-like cells and method for reprogramming adult mammalian somatic cells
EP2499241B8 (fr) 2009-11-10 2018-02-21 The J. David Gladstone Institutes Procédés de génération de cellules souches neuronales

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7314721B2 (en) * 2000-01-21 2008-01-01 Ludwig Institute For Cancer Research Small cell lung cancer associated antigens and uses therefor
US20050032207A1 (en) * 2001-09-12 2005-02-10 Anna Wobus Method for isolating, culturing and differentiating intestinal stem cells for therapeutic use
US20080260829A1 (en) * 2004-10-27 2008-10-23 New York Society For The Ruptured And Crippled Maintaining The Hospital For Special Surgery Bmp Gene and Fusion Protein
US20090191159A1 (en) * 2007-06-15 2009-07-30 Kazuhiro Sakurada Multipotent/pluripotent cells and methods

Also Published As

Publication number Publication date
EP2499241B8 (fr) 2018-02-21
EP2499241B1 (fr) 2018-01-10
EP2499241A4 (fr) 2013-08-21
US20150017139A1 (en) 2015-01-15
US9249391B2 (en) 2016-02-02
WO2011059920A2 (fr) 2011-05-19
US20120269778A1 (en) 2012-10-25
US8815589B2 (en) 2014-08-26
EP2499241A2 (fr) 2012-09-19

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