WO2011037367A3 - 고효율 유도만능줄기세포 제조방법 및 상기 방법에 의해 제조된 유도만능줄기세포 - Google Patents

고효율 유도만능줄기세포 제조방법 및 상기 방법에 의해 제조된 유도만능줄기세포 Download PDF

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Publication number
WO2011037367A3
WO2011037367A3 PCT/KR2010/006374 KR2010006374W WO2011037367A3 WO 2011037367 A3 WO2011037367 A3 WO 2011037367A3 KR 2010006374 W KR2010006374 W KR 2010006374W WO 2011037367 A3 WO2011037367 A3 WO 2011037367A3
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WO
WIPO (PCT)
Prior art keywords
stem cells
pluripotent stem
induced pluripotent
produced
cells
Prior art date
Application number
PCT/KR2010/006374
Other languages
English (en)
French (fr)
Other versions
WO2011037367A2 (ko
Inventor
박영배
김효수
권유욱
조현재
백재승
Original Assignee
서울대학교병원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020100001940A external-priority patent/KR20110032989A/ko
Application filed by 서울대학교병원 filed Critical 서울대학교병원
Priority to US13/120,331 priority Critical patent/US8673633B2/en
Publication of WO2011037367A2 publication Critical patent/WO2011037367A2/ko
Publication of WO2011037367A3 publication Critical patent/WO2011037367A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/45—Artificially induced pluripotent stem cells

Abstract

본 발명은 배아줄기세포와 동일한 분화능을 가지는 맞춤형 만능줄기세포를 제조하는 방법에 관한 것으로, 다양한 방법으로 유도된 모든 종류의 역분화 줄기세포 또는 유도만능줄기세포(induced Pluripotent Stem cells, iPS)에서 단백질을 분리하는 추출물 제조 단계; 상기 추출물을 성체세포에 주입하는 단계; 및 상기 성체세포를 배양하여 배아줄기세포와 동일한 분화능을 가지는 만능줄기세포를 유도하는 단계를 포함한다. 또한, 본 발명은 상기 방법에 의해 제조된 만능줄기세포 및 상기 만능줄기세포를 포함하는 세포 치료제에 관한 것이다. 본 발명은 종래의 만능줄기세포 제조 방법에 비하여 매우 용이하게 그리고 현저하게 높은 효율로 만능줄기세포를 제조하는 것을 가능하게 한다.
PCT/KR2010/006374 2009-09-22 2010-09-17 고효율 유도만능줄기세포 제조방법 및 상기 방법에 의해 제조된 유도만능줄기세포 WO2011037367A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/120,331 US8673633B2 (en) 2009-09-22 2010-09-17 Method for producing induced pluripotent stem cells with high efficiency and induced poluripotent stem cells prouced thereby

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2009-0089330 2009-09-22
KR20090089330 2009-09-22
KR10-2010-0001940 2010-01-08
KR1020100001940A KR20110032989A (ko) 2009-09-22 2010-01-08 성체세포로부터 만능줄기세포를 유도하는 방법 및 그 방법에 의해 제조된 만능줄기세포

Publications (2)

Publication Number Publication Date
WO2011037367A2 WO2011037367A2 (ko) 2011-03-31
WO2011037367A3 true WO2011037367A3 (ko) 2011-09-01

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PCT/KR2010/006374 WO2011037367A2 (ko) 2009-09-22 2010-09-17 고효율 유도만능줄기세포 제조방법 및 상기 방법에 의해 제조된 유도만능줄기세포

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WO (1) WO2011037367A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020530271A (ja) 2017-06-30 2020-10-22 ピーティーティー グローバル ケミカル パブリック カンパニー リミテッド 安定化されたコピー数の機能性ｄｎａ配列を有する微生物及び関連方法
CN111500529A (zh) * 2020-04-29 2020-08-07 辽宁和泽生物科技有限公司 一种从人胎盘蜕膜分离亚全能干细胞的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090130582A (ko) * 2008-06-16 2009-12-24 서울대학교병원 배아줄기세포의 추출물을 이용한 맞춤형 만능줄기세포의유도 방법 및 상기 방법에 의해 제조된 만능줄기세포
WO2011037301A1 (ko) * 2009-09-22 2011-03-31 서울대학교병원 성체세포로부터 만능줄기세포를 유도하는 방법 및 그 방법에 의해 제조된 만능줄기세포

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090130582A (ko) * 2008-06-16 2009-12-24 서울대학교병원 배아줄기세포의 추출물을 이용한 맞춤형 만능줄기세포의유도 방법 및 상기 방법에 의해 제조된 만능줄기세포
WO2011037301A1 (ko) * 2009-09-22 2011-03-31 서울대학교병원 성체세포로부터 만능줄기세포를 유도하는 방법 및 그 방법에 의해 제조된 만능줄기세포

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BRU, T. ET AL.: "Rapid induction of pluripotency genes after exposure human somatic cells to mouse ES cell extracts", EXPERIMENTAL CELL RESEARCH, vol. 314, 29 May 2008 (2008-05-29), pages 2634 - 2642 *
RAJASINGH, J. ET AL.: "Cell-free embryonic stem cell extract mediated derivation of multipotent stem cells from NIH3T3 fibroblasts for functional and anatomical ischemic tissue repair", CIRCULATION RESEARCH, vol. 102, 15 May 2008 (2008-05-15), pages E107 - E117 *

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