WO2011034704A1 - Soybean transgenic event mon 87708 and methods of use thereof - Google Patents

Soybean transgenic event mon 87708 and methods of use thereof Download PDF

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WO2011034704A1
WO2011034704A1 PCT/US2010/046759 US2010046759W WO2011034704A1 WO 2011034704 A1 WO2011034704 A1 WO 2011034704A1 US 2010046759 W US2010046759 W US 2010046759W WO 2011034704 A1 WO2011034704 A1 WO 2011034704A1
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dna
seq
soybean
plant
event mon
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PCT/US2010/046759
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French (fr)
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WO2011034704A8 (en
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Ronald J. Brinker
Wen C. Burns
Paul C.C. Feng
Anju Gupta
Sio-Wai Hoi
Marianne Malven
Kunsheng Wu
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Monsanto Technology Llc
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Priority to KR1020127009679A priority Critical patent/KR101376028B1/en
Priority to UAA201204680A priority patent/UA115761C2/en
Priority to RU2012115127A priority patent/RU2624025C2/en
Priority to IN2963DEN2012 priority patent/IN2012DN02963A/en
Priority to AP2012006217A priority patent/AP2872A/en
Priority to EP10817646.2A priority patent/EP2478000B1/en
Priority to AU2010295864A priority patent/AU2010295864C1/en
Priority to SG2012017455A priority patent/SG179103A1/en
Priority to EP21154710.4A priority patent/EP3875592A3/en
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Priority to CN2010800478901A priority patent/CN102596984A/en
Priority to NZ598724A priority patent/NZ598724A/en
Priority to ES10817646.2T priority patent/ES2675311T3/en
Priority to BR112012006079-1A priority patent/BR112012006079B1/en
Priority to MX2012003299A priority patent/MX2012003299A/en
Priority to JP2012529778A priority patent/JP5726878B2/en
Priority to DK10817646.2T priority patent/DK2478000T3/en
Priority to CA2773929A priority patent/CA2773929C/en
Priority to EP16171892.9A priority patent/EP3127425B1/en
Priority to MX2014000571A priority patent/MX351696B/en
Publication of WO2011034704A1 publication Critical patent/WO2011034704A1/en
Publication of WO2011034704A8 publication Critical patent/WO2011034704A8/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/01—Pulses or legumes in form of whole pieces or fragments thereof, without mashing or comminuting
    • A23L11/03—Soya beans, e.g. full-fat soya bean flakes or grits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Definitions

  • sequence listing that is contained in the file named "55544-0001_seqlisting.txt", which is 19.5 kilobytes (size as measured in Microsoft Windows®) and was created on August 13, 2010, is filed herewith by electronic submission and is incorporated by reference herein.
  • the invention relates to transgenic Glycine max event MON 87708.
  • the event exhibits tolerance to dicamba herbicide.
  • the invention also relates to plants, plant parts, plant seeds, plant cells, agricultural products, and methods related to event MON 87708 and provides nucleotide molecules that are unique to the event and were created in connection with the insertion of transgenic DNA into the genome of a Glycine max plant. BACKGROUND OF THE INVENTION
  • Soybean ⁇ Glycine max is an important crop in many areas of the world, and the methods of biotechnology have been applied to this crop in order to produce soybean with desirable traits.
  • One such desirable trait is herbicide tolerance.
  • the expression of an herbicide tolerance transgene in a plant can confer the desirable trait of herbicide tolerance on the plant, but expression of the transgene may be influenced by the chromosomal location and the genomic result of the transgene insertion. For example, it has been observed in plants that there often is variation in the level and pattern of transgene expression among individual events that differ in the chromosomal insertion site of the transgene but are otherwise identical. There may also be undesirable and/or desirable phenotypic or agronomic differences between events.
  • the invention provides transgenic soybean plants designated event MON 87708, which exhibit commercially acceptable tolerance to applications of dicamba herbicide, having representative seed deposited with American Type Culture Collection (ATCC) with Accession No. PTA-9670.
  • the invention also provides novel DNA molecules related to soybean event MON 87708 and methods of using these molecules.
  • the invention also provides seeds, progeny, plant parts, cells, and commodity products of soybean event MON 87708.
  • the invention also provides methods of using soybean event MON 87708 and methods of producing dicamba tolerant soybean.
  • the invention provides recombinant DNA molecules related to soybean event MON 87708. These recombinant DNA molecules may comprise nucleotide molecules having a nucleotide sequence representing a region of the genomic DNA flanking the transgene insertion, and/or a region of the transgene insertion, and/or a contiguous sequence of any of these regions such as a region of the junction between the transgene insertion and flanking genomic DNA of soybean event MON 87708.
  • the invention also provides DNA molecules useful as primers and probes diagnostic for soybean event MON 87708 and amplicons diagnostic for the presence of soybean event MON 87708. Soybean plants, plant cells, plant parts, commodity products, progeny, and seeds comprising these molecules are also disclosed.
  • the invention provides methods, compositions, and kits useful for detecting the presence and/or absence of DNA derived from soybean event MON 87708 and thus the presence and/or absence of the event.
  • the invention provides a method for detection of MON 87708 by contacting a sample comprising DNA with a primer set that when used in a nucleic acid amplification reaction with genomic DNA from soybean event MON 87708 produces an amplified DNA diagnostic for soybean event MON 87708, performing a nucleic acid amplification reaction thereby producing the amplified DNA, and detecting the presence and/or absence of the amplified DNA.
  • the invention also provides a method for detection of MON 87708 by contacting a sample comprising DNA with a probe that when used in a hybridization reaction with DNA from soybean event MON 87708 hybridizes to a DNA molecule specific for soybean event MON 87708, performing a hybridization reaction, and detecting the hybridization of the probe to the DNA molecule.
  • Kits comprising the methods and compositions of the invention useful for detecting the presence of DNA derived from soybean event MON 87708 are also provided.
  • the invention provides a soybean plant, seed, plant cell, progeny plant, plant part, or commodity product derived from a plant, plant cell, or seed of soybean event MON 87708.
  • the invention also provides a soybean plant, seed, plant cell, progeny plant, plant part, or commodity product comprising a recombinant DNA molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8, and complements and fragments thereof.
  • the invention also provides a soybean plant, seed, plant cell, progeny plant, plant part, or commodity product derived from the plant or seed of soybean event MON 87708 and comprising a recombinant DNA molecule that produces an amplified DNA molecule comprising SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8 in a DNA amplification method.
  • the invention provides a method for controlling weeds in a field by planting soybean event MON 87708 and then applying an effective dose of dicamba herbicide capable of controlling the weeds without injuring the soybean event MON 87708 plants.
  • the invention also provides a method for controlling weeds in a field by applying an effective dose of dicamba herbicide to control weeds in a field and then planting soybean event MON 87708 in the field.
  • the invention also provides a method for producing soybean seed essentially free of the seeds of toxic weed species by planting seeds of a dicamba tolerant soybean variety MON 87708 in a field, applying a post-emergence effective dose of dicamba herbicide sufficient to kill the toxic weed species to the field, and harvesting seed from the field.
  • the invention provides methods of producing a soybean plant and/or seed that tolerates application of dicamba herbicide by sexually crossing a soybean event MON 87708 plant comprising SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8 with a second soybean plant, thereby producing seed, growing the seed to produce progeny plants, treating the progeny plants with dicamba, and selecting a progeny plant that is tolerant to dicamba.
  • the methods may also include selfing the selected progeny plant to produce a plurality of second generation progeny plants and selecting from these a dicamba tolerant plant.
  • the methods may also include sexually crossing the selected progeny plant with another soybean plant to produce seed, growing the seed to produce a second generation of progeny plants, treating the second generation of progeny plants with dicamba, and selecting a second generation progeny plant that is tolerant to dicamba.
  • the invention provides methods of producing a soybean plant and/or seed that tolerates application of dicamba herbicide by selfing a dicamba tolerant soybean event MON 87708 plant comprising SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8, thereby producing seed, growing the seed to produce progeny plants, treating the progeny plants with dicamba; and selecting a progeny plant that is tolerant to dicamba.
  • the invention provides methods of determining the zygosity of a soybean event MON 87708 plant or seed comprising contacting a soybean DNA sample with a primer set comprising SEQ ID NO: 12, SEQ ID NO: 13, and SEQ ID NO: 14 and a probe set comprising SEQ ID NO: 15 and SEQ ID NO: 16; then performing a nucleic acid amplification reaction with the sample, primer set, and probe set; then detecting in then nucleic acid amplification reaction a first fluorescent signal that is diagnostic for event MON 87708 and a second fluorescent signal different from the first fluorescent signal and that is diagnostic for native soybean genomic DNA corresponding to the location of insertion of the event MON 87708 transgene; and analyzing the presence and/or absence of the first fluorescent signal and the second fluorescent signal in the nucleic acid amplification reaction, wherein the presence of both fluorescent signals indicates the sample is heterozygous for event MON 87708 and the presence of only the first fluorescent signal indicates the sample is homozygous for event MON 87708.
  • the invention also provides a soybean plant, seed, plant cell, or plant part comprising soybean haplotype region on linkage group 9 at approximately map position 143.5 comprising a dicamba tolerance gene and further defined by haplotype window 19743 and 19767, and methods of using the same.
  • Figure 1 illustrates the organization of the transgenic insert in the genome of soybean event MON 87708;
  • [A] corresponds to the relative position of SEQ ID NO: 1, which is sixty nucleotides of the junction between the soybean genomic DNA and the 5' portion of the transgene insert DNA;
  • [ ⁇ '] corresponds to the relative position of SEQ ID NO: 7, which is one hundred nucleotides of the junction between the soybean genomic DNA and the 5' portion of the transgene insert DNA;
  • [B] corresponds to the relative position of SEQ ID NO: 2, which is sixty nucleotides of the junction between the soybean genomic DNA and the 3' portion of the transgene insert DNA;
  • [ ⁇ '] corresponds to the relative position of SEQ ID NO: 8, which is one hundred nucleotides of the junction between the soybean genomic DNA and the 3 ' portion of the transgene insert DNA;
  • [C] corresponds to the relative position of SEQ ID NO: 3, which is the soybean genome sequence flanking the arbitrarily assigned/designated 5' end of
  • SEQ ID NO: 1 is a sixty nucleotide sequence representing the 5' junction between the soybean genomic DNA and the integrated transgenic expression cassette. SEQ ID NO: 1 is positioned in SEQ ID NO: 6 at nucleotide position 1097-1156.
  • SEQ ID NO: 2 is a sixty nucleotide sequence representing the 3 ' junction between the soybean genomic DNA and the integrated transgenic expression cassette. SEQ ID NO: 2 is positioned in SEQ ID NO: 6 at nucleotide position 4100-4159.
  • SEQ ID NO: 3 is the 5' sequence flanking the inserted DNA of soybean event MON 87708 up to and including a region of transgene DNA insertion.
  • SEQ ID NO: 4 is the 3 ' sequence flanking the inserted DNA of soybean event MON 87708 up to and including a region of transgene DNA insertion.
  • SEQ ID NO: 5 is the sequence of the integrated transgenic expression cassette.
  • SEQ ID NO: 6 is the nucleotide sequence representing the contig of the 5' sequence flanking the inserted DNA of soybean event MON 87708 (SEQ ID NO: 3), the sequence of the inserted DNA (SEQ ID NO: 5), and the 3' sequence flanking the inserted DNA of soybean event MON 87708 (SEQ ID NO: 4) and includes SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and SEQ ID NO: 8.
  • SEQ ID NO: 7 is a one hundred nucleotide sequence representing the 5' junction between the soybean genomic DNA and the integrated transgenic expression cassette.
  • SEQ ID NO: 8 is a one hundred nucleotide sequence representing the 3' junction between the soybean genomic DNA and the integrated transgenic expression cassette.
  • SEQ ID NO: 9 is the sequence of a primer referred to as Primer SQ13570 and used to identify soybean event MON 87708. It is complimentary to the inserted expression cassette at the region close to the 3' transgene insertion border.
  • a PCR amplicon produced from a TAQMAN® (PE Applied Biosystems, Foster City, CA) assay using the combination of primers SQ13570 and SQ13571 (SEQ ID NO: 10) is a positive result for the presence of the event MON 87708.
  • SEQ ID NO: 10 is the sequence of a primer referred to as Primer SQ13571 and used to identify soybean event MON 87708. It is complimentary to a 3 'region flanking the inserted expression cassette and close to the transgene DNA insertion border.
  • a PCR amplicon produced from a TAQMAN® (PE Applied Biosystems, Foster City, CA) assay using the combination of primers SQ13570 (SEQ ID NO: 9) and SQ13571 is a positive result for the presence of the event MON 87708.
  • SEQ ID NO: 11 is the sequence of a probe referred to as Probe PB4655 and used to identify soybean event MON 87708. It is complimentary to a region spanning the 3' junction of the inserted expression cassette and the genomic DNA.
  • This probe is a 6-FAMTM-labeled synthetic oligonucleotide. Release of a fluorescent signal in an amplification reaction using primers SQ13570 and SQ13571 (SEQ ID NO: 9-10) in combination with 6-FAMTM-labeled probe PB4655 is diagnostic of event MON 87708 in a TAQMAN® assay.
  • SEQ ID NO: 12 is the sequence of a primer referred to as Primer SQ20632 and used to identify MON 87708 event zygosity.
  • SEQ ID NO: 13 is the sequence of a primer referred to as Primer SQ20636 and used to identify soybean wild-type and MON 87708 event zygosity.
  • SEQ ID NO: 14 is the sequence of a primer referred to as Primer SQ20637 and used to identify soybean wild-type zygosity.
  • SEQ ID NO: 15 is the sequence of a probe referred to as Probe PB10130 and used for a
  • SEQ ID NO: 16 is the sequence of a probe referred to as Probe PB10131 and used for a soybean wild-type zygosity assay.
  • the invention provides a transgenic soybean event MON 87708 that exhibits commercially acceptable tolerance to applications of dicamba herbicide.
  • the event comprises a single insertion of transgenic DNA into the chromosome/genome of the soybean germplasm.
  • An "event” is produced by: (i) transformation of a plant cell with a nucleic acid construct that includes a transgene of interest, (ii) regeneration of a population of plants resulting from the insertion of the transgene into the genome of the plant, and (iii) selection of a particular plant characterized by insertion of the transgene into a particular location in the plant's genome.
  • the term "event” refers to the original transformant that includes the transgene inserted into the particular location in the plant's genome.
  • event also refers to progeny of the transformant that include the transgene inserted into the particular location in the plant's genome.
  • progeny may be produced by a sexual outcross between the transformant, or its progeny, and another plant.
  • Such other plant may be a transgenic plant comprising the same or different transgene and/or a nontransgenic plant, such as one from a different variety. Even after repeated back-crossing to a recurrent parent, the inserted DNA and flanking DNA from the transformed parent is present in the progeny of the cross at the same genomic location.
  • soybean means Glycine max and includes all plant varieties that can be bred with soybean, including wild soybean species as well as those plants belonging to Glycine that permit breeding between species.
  • the term "event” also refers to a DNA molecule from the original transformant comprising the inserted DNA and the flanking soybean genomic DNA immediately adjacent to either side of the inserted DNA.
  • This DNA molecule is created by the act of inserting the transgenic DNA into the genome of the soybean plant, i.e. , by the act of transformation.
  • This DNA molecule therefore comprises a nucleotide sequence that is both specific to the event and that is unique to the genome of the soybean plant into which the transgenic DNA has been inserted, in that this nucleotide sequence contains both the sequence of a particular region of soybean genomic DNA and of the transgenic DNA insert.
  • the arrangement of the inserted DNA in soybean event MON 87708 in relation to the surrounding soybean plant genome DNA is therefore specific and unique for soybean event MON 87708.
  • This DNA molecule is also an integral part of the soybean chromosome of event MON 87708 and as such is static in the plant and may be passed on to progeny of the plant.
  • Event MON 87708 comprises a transgene that confers tolerance to applications of dicamba herbicide to the soybean plant.
  • Dicamba refers to 3,6-dichloro-2-methoxybenzoic acid.
  • Dicamba is a synthetic auxin herbicide useful for controlling broadleaf weeds.
  • Soybean plants were transformed with dicamba mono-oxygenase (DMO), an enzyme cloned from Stenotrophomonas maltophilia which is commonly found in soil rhizosphere.
  • Dicamba mono-oxygenase is an enzyme that catalyzes the deactivation of dicamba via an O-demethylation reaction to the nonherbicidal compound 3,5- dichlorosalicylic acid.
  • toxic weed species seeds may contaminate harvested soybean seeds that can affect the health and nutrition of animals fed the contaminated soybean commodity products. These plants can be eliminated from a soybean field by treatment with a dicamba herbicide.
  • a dicamba herbicide included in this group of toxic weeds.
  • Members of this group of toxic weeds include Cardaria spp, Heliotropium spp, Centaurea spp., Senecio spp., Crotalaria spp., Solanum spp., Xanthium spp., Amsinckia spp., Cassia spp., Sesbania spp., Datura spp., Ricinus spp., Argemone spp., Corchorus spp., Impomoea spp., and Echium spp.
  • recombinant refers to a form of DNA and/or protein and/or an organism that would not normally be found in nature and as such was created by human intervention. Such human intervention may produce a recombinant DNA molecule and/or a recombinant plant.
  • a "recombinant DNA molecule” is a DNA molecule comprising a combination of DNA molecules that would not naturally occur together and is the result of human intervention, e.g., a DNA molecule that is comprised of a combination of at least two DNA molecules heterologous to each other, and/or a DNA molecule that is artificially synthesized and comprises a polynucleotide sequence that deviates from the polynucleotide sequence that would normally exist in nature, and/or a DNA molecule that comprises a transgene artificially incorporated into a host cell's genomic DNA and the associated flanking DNA of the host cell's genome.
  • a recombinant DNA molecule is a DNA molecule described herein resulting from the insertion of the transgene into the soybean genomic DNA, which may ultimately result in the expression of a recombinant RNA and/or protein molecule in that organism.
  • a "recombinant plant” is a plant that would not normally exist in nature, is the result of human intervention, and contains a transgene and/or heterologous DNA molecule incorporated into its genome. As a result of such genomic alteration, the recombinant plant is distinctly different from the related wildtype plant.
  • An example of a recombinant plant is a soybean plant described herein as Event MON 87708.
  • transgene refers to a nucleotide molecule artificially incorporated into a host cell's genome. Such transgene may be heterologous to the host cell.
  • transgenic plant refers to a plant comprising such a transgene.
  • heterologous refers to a first molecule not normally found in combination with a second molecule in nature.
  • a molecule may be derived from a first species and inserted into the genome of a second species. The molecule would thus be heterologous to the host and artificially incorporated into a host cell's genome.
  • chimeric refers to a single DNA molecule produced by fusing a first DNA molecule to a second DNA molecule, where neither first nor second DNA molecule would normally be found in that configuration, i.e., fused to the other.
  • the chimeric DNA molecule is thus a new DNA molecule not otherwise normally found in nature.
  • DNA molecules and their corresponding nucleotide sequences.
  • DNA DNA molecule
  • nucleotide molecule refers to a DNA molecule of genomic or synthetic origin, i.e., a polymer of deoxyribonucleotide bases or a polynucleotide molecule, read from the 5' (upstream) end to the 3' (downstream) end.
  • DNA sequence nucleotide sequence or polynucleotide sequence
  • DNA sequence refers to the nucleotide sequence of a DNA molecule.
  • the nucleotide sequence corresponding to the complete nucleotide sequence of the inserted transgenic DNA and substantial segments of the soybean genome DNA flanking either end of the inserted transgenic DNA is provided herein as SEQ ID NO: 6.
  • a subsection of this is the inserted transgenic DNA provided as SEQ ID NO: 5.
  • the nucleotide sequence of the soybean genome DNA physically linked by phosphodiester bond linkage to and therefore flanking the 5 ' end of the inserted transgenic DNA is set forth as shown in SEQ ID NO: 3.
  • the nucleotide sequence of the soybean genome DNA physically linked by phosphodiester bond linkage to and therefore flanking the 3 ' end of the inserted transgenic DNA is set forth as shown in SEQ ID NO: 4.
  • the soybean event MON 87708 further comprises two regions, one spanning the 5' location and one spanning the 3 ' location where the transgenic DNA is inserted into the genomic DNA, referred to herein as the 5' and 3' junction, respectively.
  • a "junction sequence” or “junction region” refers to the DNA sequence and/or corresponding DNA molecule that spans the inserted transgenic DNA and the adjacent flanking genomic DNA.
  • the junction sequences may be arbitrarily represented by the two 60 nucleotide sequences provided as SEQ ID NO: 1 and SEQ ID NO: 2, each representing 30 nucleotides of the flanking genomic DNA adjacent to and contiguous with 30 nucleotides of insert DNA.
  • junction sequences may be arbitrarily represented by the two 100 nucleotide sequences provided as SEQ ID NO: 7 and SEQ ID NO: 8, each representing 50 nucleotides of the flanking genomic DNA adjacent to and contiguous with 50 nucleotides of insert DNA. These nucleotides are connected by phosphodiester linkage and in soybean event MON 87708 are present as part of the genome.
  • the identification of one or more of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 7, and SEQ ID NO: 8 in a sample derived from a soybean plant, seed, or plant part is determinative that the DNA was obtained from soybean event MON 87708 and is diagnostic for the presence in a sample of DNA from soybean event MON 87708.
  • the invention thus provides a DNA molecule that contains at least the nucleotide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8.
  • Any segment of DNA derived from transgenic soybean event MON 87708 that is sufficient to include SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8 is within the scope of The invention.
  • any polynucleotide comprising a sequence complementary to any of the sequences described within this paragraph is within the scope of the invention.
  • Figure 1 illustrates the physical arrangement of SEQ ID NO: 1-5 and 7-8 relative to SEQ ID NO: 6 arranged from 5'to 3'.
  • the invention provides exemplary DNA molecules that can be used either as primers or probes for diagnosing the presence of DNA derived from soybean plant event MON 87708 in a sample.
  • primers or probes are specific for a target nucleic acid sequence and as such are useful for the identification of soybean event MON 87708 nucleic acid sequence by the methods of the invention described herein.
  • a "primer” is typically a highly purified, isolated polynucleotide that is designed for use in specific annealing or hybridization methods that involve thermal amplification.
  • a pair of primers may be used with template DNA, such as a sample of soybean genomic DNA, in a thermal amplification, such as polymerase chain reaction (PCR), to produce an amplicon, where the amplicon produced from such reaction would have a DNA sequence corresponding to sequence of the template DNA located between the two sites where the primers hybridized to the template.
  • PCR polymerase chain reaction
  • an "amplicon” is a piece or fragment of DNA that has been synthesized using amplification techniques.
  • An amplicon of the invention comprises at least SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8.
  • a primer is typically designed to hybridize to a complementary target DNA strand to form a hybrid between the primer and the target DNA strand, and the presence of the primer is a point of recognition by a polymerase to begin extension of the primer (i.e., polymerization of additional nucleotides into a lengthening nucleotide molecule) using as a template the target DNA strand.
  • Primer pairs are intended to refer to use of two primers binding opposite strands of a double stranded nucleotide segment for the purpose of amplifying linearly the polynucleotide segment between the positions targeted for binding by the individual members of the primer pair, typically in a thermal amplification reaction or other conventional nucleic-acid amplification methods.
  • Exemplary DNA molecules useful as primers are provided as SEQ ID NO: 9-10.
  • the primer pair provided as SEQ ID NO: 9 and SEQ ID NO: 10 are useful as a first DNA molecule and a second DNA molecule that is different from the first DNA molecule, and both are each of sufficient length of contiguous nucleotides of either SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6 to function as DNA primers that, when used together in a thermal amplification reaction with template DNA derived from soybean event MON 87708, produce an amplicon comprising SEQ ID NO: 2.
  • a “probe” is an isolated nucleic acid that is complementary to a strand of a target nucleic acid.
  • Probes according to the invention include not only deoxyribonucleic or ribonucleic acids but also polyamides and other probe materials that bind specifically to a target DNA sequence and the detection of such binding can be useful in diagnosing, discriminating, determining, or confirming the presence of that target DNA sequence in a particular sample.
  • a probe may be attached to a conventional detectable label or reporter molecule, e.g., a radioactive isotope, ligand, chemiluminescent agent, or enzyme.
  • An exemplary DNA molecule useful as a probe is provided as SEQ ID NO: 11.
  • Probes and primers according to the invention may have complete sequence identity with the target sequence, although primers and probes differing from the target sequence that retain the ability to hybridize preferentially to target sequences may be designed by conventional methods.
  • primers and probes differing from the target sequence that retain the ability to hybridize preferentially to target sequences may be designed by conventional methods.
  • a nucleic acid molecule In order for a nucleic acid molecule to serve as a primer or probe it need only be sufficiently complementary in sequence to be able to form a stable double -stranded structure under the particular solvent and salt concentrations employed. Any conventional nucleic acid hybridization or amplification method can be used to identify the presence of transgenic DNA from soybean event MON 87708 in a sample.
  • Probes and primers are generally at least about 11 nucleotides, at least about 18 nucleotides, at least about 24 nucleotides, or at least about 30 nucleotides or more in length. Such probes and primers hybridize specifically to a target DNA sequence under stringent hybridization conditions. Conventional stringency conditions are described by Sambrook et ah, 1989, and by Haymes et al , In: Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, DC (1985). As used herein, two nucleic acid molecules are capable of specifically hybridizing to one another if the two molecules are capable of forming an anti-parallel, double-stranded nucleic acid structure.
  • a nucleic acid molecule is the "complement” of another nucleic acid molecule if they exhibit complete complementarity.
  • molecules exhibit “complete complementarity” when every nucleotide of one of the molecules is complementary to a nucleotide of the other.
  • Two molecules are “minimally complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional "low- stringency” conditions.
  • the molecules are “complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional "high-stringency” conditions. Departures from complete complementarity are therefore permissible, as long as such departures do not completely preclude the capacity of the molecules to form a double-stranded structure.
  • isolated refers to at least partially separating a molecule from other molecules normally associated with it in its native or natural state.
  • the term “isolated” refers to a DNA molecule that is at least partially separated from the nucleic acids which normally flank the DNA molecule in its native or natural state.
  • DNA molecules fused to regulatory or coding sequences with which they are not normally associated, for example as the result of recombinant techniques are considered isolated herein. Such molecules are considered isolated even when integrated into the chromosome of a host cell or present in a nucleic acid solution with other DNA molecules.
  • DNA molecules, or fragment thereof can also be obtained by other techniques such as by directly synthesizing the fragment by chemical means, as is commonly practiced by using an automated oligonucleotide synthesizer.
  • DNA molecules and corresponding nucleotide sequences provided herein are therefore useful for, among other things, identifying soybean event MON 87708, selecting plant varieties or hybrids comprising soybean event MON 87708, detecting the presence of DNA derived from the transgenic soybean event MON 87708 in a sample, and monitoring samples for the presence and/or absence of soybean event MON 87708 or plant parts derived from soybean event MON 87708.
  • the invention provides soybean plants, progeny, seeds, plant cells, plant parts (such as pollen, ovule, pod, flower tissue, root tissue, stem tissue, and leaf tissue), and commodity products.
  • These plants, progeny, seeds, plant cells, plant parts, and commodity products contain a detectable amount of a polynucleotide of the invention, i.e., such as a polynucleotide having at least one of the sequences provided as SEQ ID NO: 1-8.
  • Plants, progeny, seeds, plant cells, and plant parts of the invention may also contain one or more additional transgenes.
  • transgene may be any nucleotide sequence encoding a protein or RNA molecule conferring a desirable trait including but not limited to increased insect resistance, increased water use efficiency, increased yield performance, increased drought resistance, increased seed quality, improved nutritional quality, and/or increased herbicide tolerance, in which the desirable trait is measured with respect to a soybean plant lacking such additional transgene.
  • the invention provides soybean plants, progeny, seeds, plant cells, and plant part such as pollen, ovule, pod, flower, root or stem tissue, and leaves derived from a transgenic soybean plant event MON 87708.
  • a representative sample of soybean event MON 87708 seed has been deposited according to the Budapest Treaty for the purpose of enabling the invention.
  • the repository selected for receiving the deposit is the American Type Culture Collection (ATCC) having an address at 10801 University Boulevard, Manassas, Virginia USA, Zip Code 20110.
  • the ATCC repository has assigned the accession No. PTA-9670 to the event MON 87708 seed.
  • the invention provides a microorganism comprising a DNA molecule having SEQ ID NO: 1 and SEQ ID NO: 2 present in its genome.
  • An example of such a microorganism is a transgenic plant cell.
  • Microorganisms, such as a plant cell of The invention are useful in many industrial applications, including but not limited to: (i) use as research tool for scientific inquiry or industrial research; (ii) use in culture for producing endogenous or recombinant carbohydrate, lipid, nucleic acid, or protein products or small molecules that may be used for subsequent scientific research or as industrial products; and (iii) use with modern plant tissue culture techniques to produce transgenic plants or plant tissue cultures that may then be used for agricultural research or production.
  • microorganisms such as transgenic plant cells utilizes modern microbiological techniques and human intervention to produce a man-made, unique microorganism.
  • recombinant DNA is inserted into a plant cell's genome to create a transgenic plant cell that is separate and unique from naturally occurring plant cells.
  • This transgenic plant cell can then be cultured much like bacteria and yeast cells using modern microbiology techniques and may exist in an undifferentiated, unicellular state.
  • the new plant cell's genetic composition and phenotype is a technical effect created by the integration of the heterologous DNA into the genome of the cell.
  • Another aspect of the invention is a method of using a microorganism of the invention.
  • Methods of using microorganisms of the invention include (i) methods of producing transgenic cells by integrating recombinant DNA into the genome of the cell and then using this cell to derive additional cells possessing the same heterologous DNA; (ii) methods of culturing cells that contain recombinant DNA using modern microbiology techniques; (iii) methods of producing and purifying endogenous or recombinant carbohydrate, lipid, nucleic acid, or protein products from cultured cells; and (iv) methods of using modern plant tissue culture techniques with transgenic plant cells to produce transgenic plants or transgenic plant tissue cultures.
  • Plants of the invention may pass along the event DNA, including the transgene, to progeny.
  • progeny includes any plant, seed, plant cell, and/or regenerable plant part comprising the event DNA derived from an ancestor plant and/or a polynucleotide having at least one of the sequences provided as SEQ ID NO: 1 and SEQ ID NO: 2.
  • Plants, progeny, and seeds may be homozygous or heterozygous for the transgene.
  • Progeny may be grown from seeds produced by a soybean event MON 87708 plant and/or from seeds produced by a plant fertilized with pollen from a soybean event MON 87708 plant.
  • Progeny plants may be self-pollinated (also known as "selfing") to generate a true breeding line of plants, i.e., plants homozygous for the transgene. Selfing of appropriate progeny can produce plants that are homozygous for both added, exogenous genes.
  • progeny plants may be outcrossed, e.g., bred with another unrelated plant, to produce a varietal or a hybrid seed or plant.
  • the other unrelated plant may be transgenic or nontransgenic.
  • a varietal or hybrid seed or plant of the invention may thus be derived by crossing a first parent that lacks the specific and unique DNA of the soybean event MON 87708 with a second parent comprising soybean event MON 87708, resulting in a hybrid comprising the specific and unique DNA of the soybean event MON 87708.
  • Each parent can be a hybrid or an inbred/varietal, so long as the cross or breeding results in a plant or seed of the invention, i.e., a seed having at least one allele containing the specific and unique DNA of soybean event MON 87708 and/or SEQ ID NO: 1 and SEQ ID NO: 2.
  • Two different transgenic plants may thus be mated to produce hybrid offspring that contain two independently segregating, added, exogenous genes.
  • the MON 87708 dicamba tolerant soybean can be crossed with other transgenic soybean plant to produce a plant having the characteristics of both transgenic parents.
  • One example of this would be a cross of MON 87708 dicamba tolerant soybean with a plant having one or more additional traits such as herbicide tolerance (e.g.
  • soybean event 40-3-2 or soybean event MON89788 (U.S. Patent Application Publication No. 20060282915)), insect control (e.g. soybean event MON87701 (U.S. Patent Application Publication No. 20090130071)), and/or other desirable traits (e.g. enhanced oil composition such as soybean event MON87769 (PCT Patent Publication WO2009102873)), resulting in a progeny plant or seed that is tolerant to dicamba and has one or more additional traits.
  • Herbicides for which transgenic plant tolerance has been demonstrated and the method of the invention can be applied include but are not limited to: glyphosate, glufosinate, sulfonylureas, imidazolinones, bromoxynil, delapon, cyclohexanedione, protoporphyrionogen oxidase inhibitors, and isoxasflutole herbicides.
  • Nucleotide molecules encoding proteins involved in herbicide tolerance include, but are not limited to, a nucleotide molecule encoding: glyphosate-tolerant 5-enolpyruvylshikimate-3- phosphate synthase (EPSPS) (see, for example, U.S. Patent Nos. 5,627,061 ; 5,633,435; 6,040,497; 5,094,945; 5,804,425; 6,248,876; 7,183,110; RE39,247); glyphosate oxidoreductase (GOX) (see, for example, U.S. Patent No.
  • EPSPS glyphosate-tolerant 5-enolpyruvylshikimate-3- phosphate synthase
  • GOX glyphosate oxidoreductase
  • glyphosate-n-acetyltransferase GAT
  • GAT glyphosate-n-acetyltransferase
  • ALS herbicide-tolerant acetolactate synthase
  • AHAS acetohydroxyacid synthase
  • sulfonylureas imidazolinones, triazolopyrimidines, pyrimidinyl oxybenzoates, sulfonylamino carbonyl triazolinones, and/or heteroaryl ethers
  • ACCase or R-2,4-dichlorophenoxypropionate dioxygenase (rdpA) for tolerance to an aryloxyphenoxypropionate (AOPP) (such as haloxyfop, quizalofop, dichlorofop, and diclofop)
  • a detoxification protein such as a 2,4-D dioxygenase (tfdA), R
  • Patent No. 4,810,648 a phytoene desaturase (crtl) for tolerance to norflurazon; the bialaphos resistance (bar) or phosphinothricin acetyltransferase (PAT) protein ⁇ see, for example, U.S. Patent No.
  • glufosinate and bialaphos for tolerance to glufosinate and bialaphos
  • a protein for triketone herbicide -tolerance such as tolerant 4-HydroxyPhenylPyruvate Dioxygenase (HPPD), a detoxifying cytochrome P450, or an HPPD pathway bypass such as Artbrobacter globiformis HPP oxidase (HPPO) and Pseudomonas acidovorans 4-HPA 1 -hydroxylase (HP AH) and NADH oxidoreductase (HP AC).
  • HPPD 4-HydroxyPhenylPyruvate Dioxygenase
  • HPPO Artbrobacter globiformis HPP oxidase
  • HP AH Pseudomonas acidovorans 4-HPA 1 -hydroxylase
  • the invention provides a plant part that is derived from soybean event MON 87708.
  • a "plant part” refers to any part of a plant which is comprised of material derived from a soybean event MON 87708 plant.
  • Plant parts include but are not limited to pollen, ovule, pod, flower, root or stem tissue, fibers, and leaves. Plant parts may be viable, nonviable, regenerable, and/or nonregenerable.
  • the invention provides a commodity product that is derived from soybean event MON 87708.
  • a "commodity product” refers to any composition or product which is comprised of material derived from a soybean event MON 87708 plant, seed, plant cell, or plant part.
  • Commodity products may be sold to consumers and may be viable or nonviable.
  • Nonviable commodity products include but are not limited to nonviable seeds and grains; processed seeds, seed parts, and plant parts; dehydrated plant tissue, frozen plant tissue, and processed plant tissue; seeds and plant parts processed for animal feed for terrestrial and/or aquatic animals consumption, oil, meal, flour, flakes, bran, fiber, milk, cheese, paper, cream, wine, and any other food for human consumption; and biomasses and fuel products.
  • Viable commodity products include but are not limited to seeds and plant cells.
  • the soybean event MON 87708 can thus be used to manufacture any commodity product typically acquired from soybean.
  • Any such commodity product that is derived from the soybean event MON 87708 may contain at least a detectable amount of the specific and unique DNA corresponding to soybean event MON 87708, and specifically may contain a detectable amount of a polynucleotide containing at least 15 contiguous nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2.
  • Any standard method of detection for nucleotide molecules may be used, including methods of detection disclosed herein.
  • a commodity product is within the scope of the invention if there is any detectable amount of SEQ ID NO: 1 or SEQ ID NO: 2 in the commodity product.
  • the plants, progeny, seeds, plant cells, plant parts (such as pollen, ovule, pod, flower, root or stem tissue, and leaves), and commodity products of the invention are therefore useful for, among other things, growing plants for the purpose of producing seed and/or plant parts of soybean event MON 87708 for agricultural purposes, producing progeny of soybean event MON 87708 for plant breeding and research purposes, use with microbiological techniques for industrial and research applications, and sale to consumers.
  • the invention provides methods for controlling weeds and methods for producing plants using dicamba herbicide and soybean event MON 87708.
  • a method for controlling weeds in a field is provided and consists of planting soybean event MON 87708 varietal or hybrid plants in a field and applying a herbicidally effective dose of dicamba to the field for the purpose of controlling weeds in the field without injuring the MON 87708 plants.
  • Such application of dicamba herbicide may be pre -emergence, i.e. , any time after MON 87708 seed is planted and before MON 87708 plants emerge, or post-emergence, i.e., any time after MON 87708 plants emerge.
  • Another method for controlling weeds in a field consists of applying an effective dose of dicamba herbicide to control weeds in a field and then planting soybean event MON 87708 in the field.
  • Such application of dicamba herbicide would be pre-planting, i.e., before MON 87708 seed is planted, and could be done any time pre-planting including, but not limited to, about 14 days pre-planting to about 1 day pre-planting.
  • the invention also provides a method for producing soybean seed essentially free of the seeds of toxic weed species by planting seeds of a dicamba tolerant soybean variety MON 87708 in a field, applying a post-emergence effective dose of dicamba herbicide sufficient to kill the toxic weed species to the field, and harvesting seed from the field.
  • a herbicidally effective dose of dicamba for use in the field should consist of a range from about 0.005 pounds per acre to about 8 pounds of dicamba per acre over a growing season.
  • Multiple applications of dicamba may be used over a growing season, for example, two applications (such as a pre-planting application and a post-emergence application or a pre-emergence application and a post-emergence application) or three applications (such as a pre-planting application, a pre-emergence application, and a post-emergence application).
  • Transgenic plants used in these methods may be homozygous or heterozygous for the transgene.
  • Progeny plants produced by these methods may be varietal or hybrid plants; may be grown from seeds produced by a soybean event MON 87708 plant and/or from seeds produced by a plant fertilized with pollen from a soybean event MON 87708 plant; and may be homozygous or heterozygous for the transgene.
  • Progeny plants may be subsequently self-pollinated to generate a true breeding line of plants, i.e. , plants homozygous for the transgene, or alternatively may be outcrossed, e.g., bred with another unrelated plant, to produce a varietal or a hybrid seed or plant.
  • a soybean plant that tolerates application of dicamba herbicide may be produced by sexually crossing an event MON 87708 plant comprising a nucleotide molecule comprising the sequence of SEQ ID NO: 1 and SEQ ID NO: 2 with another soybean plant and thereby producing seed, which is then grown into progeny plants. These progeny plants may then be treated with dicamba herbicide to select for progeny plants that are tolerant to dicamba herbicide. Alternatively, these progeny plants may be analyzed using diagnostic methods to select for progeny plants that contain the event MON 87708 DNA. The other plant used in the crossing may or may not be tolerant to dicamba herbicide and may or may not be transgenic. The progeny plant and/or seed produced may be varietal or hybrid seed.
  • the step of sexually crossing one plant with another plant may be accomplished or facilitated by human intervention, for example: by human hands collecting the pollen of one plant and contacting this pollen with the style or stigma of a second plant; by human hands and/or actions removing, destroying, or covering the stamen or anthers of a plant (e.g., by detasseling or by application of a chemical gametocide) so that natural self-pollination is prevented and cross-pollination would have to take place in order for fertilization to occur; by human placement of pollinating insects in a position for "directed pollination" (e.g., by placing beehives in orchards or fields or by caging plants with pollinating insects); by human opening or removing of parts of the flower to allow for placement or contact of foreign pollen on the style or stigma (e.g.
  • a soybean plant that tolerates application of dicamba herbicide may be produced by selfing an event MON 87708 plant comprising a nucleotide molecule comprising the sequence of SEQ ID NO: 1 and SEQ ID NO: 2 and thereby producing seed, which is then grown into progeny plants. These progeny plants may then be treated with dicamba herbicide to select for progeny plants that are tolerant to dicamba herbicide. Alternatively, these progeny plants may be analyzed using diagnostic methods to select for progeny plants that contain the event MON 87708 DNA. In practicing this method, the step of sexually crossing one plant with itself, i.e.
  • self-pollinating or selfing may be accomplished or facilitated by human intervention, for example: by human hands collecting the pollen of the plant and contacting this pollen with the style or stigma of the same plant and then optionally preventing further fertilization of the plant; by human hands and/or actions removing, destroying, or covering the stamen or anthers of other nearby plants (e.g.
  • Progeny soybean plants and seeds encompassed by these methods and produced by using these methods will be distinct from other soybean plants, for example because the progeny soybean plants and seeds: are recombinant and as such created by human intervention; are dicamba herbicide tolerant; contain at least one allele that consists of the transgene DNA of the invention; and/or contain a detectable amount of a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO: 2.
  • a seed may be selected from an individual progeny plant, and so long as the seed comprises SEQ ID NO: 1 and SEQ ID NO: 2, it will be within the scope of the invention.
  • two different transgenic plants can be crossed to produce hybrid offspring that contain two independently segregating heterologous genes. Selling of appropriate progeny can produce plants that are homozygous for both genes.
  • Back-crossing to a parental plant and out-crossing with a non-transgenic plant are also contemplated, as is vegetative propagation. Descriptions of other methods that are commonly used for different traits and crops can be found in one of several references, e.g., Fehr, in Breeding Methods for Cultivar Development, Wilcox J. ed., American Society of Agronomy, Madison WI (1987).
  • transgenes may be any nucleotide sequence encoding a protein or RNA molecule conferring a desirable trait including but not limited to increased insect resistance, increased water use efficiency, increased yield performance, increased drought resistance, increased seed quality, improved nutritional quality, and/or increased herbicide tolerance, in which the desirable trait is measured with respect to a soybean plant lacking such additional transgene.
  • the methods of the invention are therefore useful for, among other things, controlling weeds in a field while growing plants for the purpose of producing seed and/or plant parts of soybean event MON 87708 for agricultural or research purposes, selecting for progeny of soybean event MON 87708 for plant breeding or research purposes, and producing progeny plants and seeds of soybean event MON 87708.
  • the plants, progeny, seeds, plant cells, plant parts (such as pollen, ovule, pod, flower, root or stem tissue, and leaves), and commodity products of the invention may be evaluated for DNA composition, gene expression, and/or protein expression. Such evaluation may be done by using any standard method such as PCR, northern blotting, southern analysis, western blotting, immuno-precipitation, and ELISA or by using the methods of detection and/or the detection kits provided herein. Methods of detecting the presence of DNA derived from a soybean cell, tissue, seed, or plant of soybean event MON 87708 in a sample are provided.
  • One method consists of (i) extracting a DNA sample from at least one soybean cell, tissue, seed, or plant, (ii) contacting the DNA sample with a primer pair that is capable of producing an amplicon from event MON 87708 DNA under conditions appropriate for DNA amplification, (iii) performing a DNA amplification reaction, and then (iv) detecting the amplicon molecule and/or confirming that the nucleotide sequence of the amplicon comprises a nucleotide sequence specific for event MON 87708, such as one selected from the group consisting of SEQ ID NO: 1-8.
  • the amplicon should be one that is specific for event MON 87708, such as an amplicon that comprises SEQ ID NO: 1 or SEQ ID NO: 2.
  • the detection of a nucleotide sequence specific for event MON 87708 in the amplicon is determinative and/or diagnostic for the presence of the soybean event MON 87708 specific DNA in the sample.
  • An example of a primer pair that is capable of producing an amplicon from event MON 87708 DNA under conditions appropriate for DNA amplification is provided as SEQ ID NO: 10-11.
  • Other primer pairs may be readily designed by one of skill in the art and would comprise at least one fragment of SEQ ID NO: 6.
  • Another method of detecting the presence of DNA derived from a soybean cell, tissue, seed, or plant of soybean event MON 87708 in a sample consists of (i) extracting a DNA sample from at least one soybean cell, tissue, seed, or plant,, (ii) contacting the DNA sample with a DNA probe specific for event MON 87708 DNA, (iii) allowing the probe and the DNA sample to hybridize under stringent hybridization conditions, and then (iv) detecting hybridization between the probe and the target DNA sample.
  • An example of the sequence a DNA probe that is specific for event MON 87708 DNA is provided as SEQ ID NO: 11.
  • Other probes may be readily designed by one of skill in the art and would comprise at least one fragment of SEQ ID NO: 6. Detection of probe hybridization to the DNA sample is diagnostic for the presence of soybean event MON 87708 specific DNA in the sample. Absence of hybridization is alternatively diagnostic of the absence of soybean event MON 87708 specific DNA in the sample.
  • DNA detection kits are provided that are useful for the identification of soybean event MON 87708 DNA in a sample and can also be applied to methods for breeding soybean plants containing the appropriate event DNA.
  • Such kits contain DNA primers and/or probes comprising fragments of SEQ ID NO: 1-8.
  • One example of such a kit comprises at least one DNA molecule of sufficient length of contiguous nucleotides of SEQ ID NO: 6 to function as a DNA probe useful for detecting the presence and/or absence of DNA derived from transgenic soybean event MON 87708 in a sample.
  • the DNA derived from transgenic soybean event MON 87708 would comprise SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8.
  • a DNA molecule sufficient for use as a DNA probe is provided that is useful for determining, detecting, or diagnosing the presence and/or absence of soybean event MON 87708 DNA in a sample is provided as SEQ ID NO: 11.
  • Other probes may be readily designed by one of skill in the art and should comprise at least 15 contiguous nucleotides of SEQ ID NO: 6 and be sufficiently unique to soybean event MON 87708 DNA in order to identify DNA derived from the event.
  • Another type of kit comprises a primer pair useful for producing an amplicon useful for detecting the presence and/or absence of DNA derived from transgenic soybean event MON 87708 in a sample.
  • Such a kit would employ a method comprising contacting a target DNA sample with a primer pair as described herein, then performing a nucleic acid amplification reaction sufficient to produce an amplicon comprising SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8, and then detecting the presence and/or absence of the amplicon.
  • a method may also include sequencing the amplicon or a fragment thereof, which would be determinative of, i.e. diagnostic for, the presence of the soybean event MON 87708 specific DNA in the target DNA sample.
  • Other primer pairs may be readily designed by one of skill in the art and should comprise at least 15 contiguous nucleotides of SEQ ID NO: 6 and be sufficiently unique to soybean event MON 87708 DNA in order to identify DNA derived from the event.
  • Nucleic-acid amplification can be accomplished by any of the various nucleic-acid amplification methods known in the art, including thermal amplification methods. Many techniques are known in the art for detecting, quantifying, and/or sequencing the amplicon produced by these methods. One exemplary technique useful in practicing this invention is TAQMAN® (PE Applied Biosystems, Foster City, CA).
  • kits and detection methods of the invention are useful for, among other things, identifying soybean event MON 87708, selecting plant varieties or hybrids comprising soybean event MON 87708, detecting the presence of DNA derived from the transgenic soybean event MON 87708 in a sample, and monitoring samples for the presence and/or absence of soybean event MON 87708 or plant parts derived from soybean event MON 87708.
  • sequence of the heterologous DNA insert, junction sequences, or flanking sequences from soybean event MON 87708 can be verified (and corrected if necessary) by amplifying such sequences from the event using primers derived from the sequences provided herein followed by standard DNA sequencing of the amplicon or of the cloned DNA.
  • soybean plant MON 87708 was produced by Agrobacterium-mediated transformation of soybean. Soybean cells were transformed and regenerated into intact soybean plants and individual plants were selected from the population of plants that showed integrity of the plant expression cassette and resistance to dicamba. From this population, soybean plant event MON 87708 was selected and characterized.
  • the transgenic dicamba tolerant soybean plant MON 87708 was developed through Agrobacterium-mediated transformation of soybean meristem tissue utilizing transformation vector PV- GMHT4355. The method was described in U.S. Patent No. 6,384,301 (herein incorporated by reference), which allows for the generation of transformed plants without utilization of callus. Briefly, meristem tissues were excised from the embryos of germinated A3525 soybean seed (Asgrow, St Louis, MO).
  • the meristems were placed on selection medium containing glyphosate (Monsanto, St Louis, MO), carbenicillin disodium salt, cefotaxime sodium salt, and ticarcillin disodium salt/potassium clavulanate mixture to inhibit the growth of untransformed plant cells and excess Agrobacterium.
  • the meristems were then placed in media conducive to shoot and root development. Rooted plants with normal phenotypic characteristics were selected and transferred to soil for growth and further assessment.
  • the R0 plants generated through the above transformation were transferred to soil for growth and then selfed to produce Rl seed.
  • the unlinked insertions of T-DNA I (dmo expression cassette) and T-DNA II (cp4 epsps expression cassette) were segregated.
  • a non-lethal dose of glyphosate was applied to Rl plants.
  • the plants with minor injuries were selected for further analyses, whereas plants showing no injury, i.e., containing T-DNA II (cp4 epsps expression cassette) were eliminated from subsequent development.
  • R0 plants containing only a single T-DNA I insert i.e., dmo gene cassette
  • the T-DNA I expression cassette comprised the Peanut Chlorotic Streak Virus (PC1SV) promoter with a duplicated enhancer region (P-PClSV.FLt-enh); operably linked to a DNA leader derived from RNA transcript of Tobacco Etch Virus (L-TEV); operably linked to a DNA molecule encoding an N-terminal chloroplast transit peptide from ribulose 1,5-bisphosphate carboxylase small subunit (SSU) from Pisum sativum (TS-RbcS-3C); operably linked to part of the mature protein from ribulose 1,5-bisphosphate carboxylase small subunit (SSU) from Pisum sativum (CR-RbcS-3C); operably linked to a DNA molecule encoding a dicamba mono-oxygenase (DMO) from Stenotrophomonas maltophilia (Pseudomonas maltophilia was the original name of the source of the DMO gene.
  • This source organism was subsequently reclassified first as Xanthomonas maltophilia and then as Stenotrophomonas maltophilia); operably linked to a 3 ' UTR DNA molecule derived from the ribulose 1,5-bisphosphate carboxylase small subunit gene of Pisum sativum (T-Ps.RbcS2- E9). Plants were selected by a combination of analytical techniques, including TaqMan, PCR analysis, and herbicide spray. The MON 87708 event was selected from among approximately 2,400 individual transgenic events based on its superior phenotypic characteristics, a comprehensive molecular profile analysis, and its desirable haplotype association.
  • Event MON 87708 was then crossed with event MON 89788 (glyphosate tolerant).
  • the progeny of this cross were treated with dicamba (Clarity®, BASF, Research Triangle Park, NC), glyphosate (Roundup WeatherMAX®, Monsanto Co., St Louis, MO), or a combination of dicamba and glyphosate.
  • the treatments were done at pre-plant, post-plant at the vegetative 3 growth stage (V3), and post-plant at the reproduction 1 stage (Rl).
  • Treated plants were scored for percent growth inhibition at 14 days after treatment (DAT) for the pre-plant herbicide treatment, 3 DAT for post-emergence treatment at the VE stage, and 3 DAT post-emergence treatment at the Rl stage.
  • the herbicide(s) were applied at various rates per acre as shown in Table 1. Percent inhibition measurements represent an average of the repetitions.
  • Table 1 Dicamba and/or Roundup WeatherMAX® tolerance testing with MON89788 x MON 87708
  • the dicamba tolerance transgene was mapped in soybean event MON 87708 to linkage group 9 at approximately map position 143.5.
  • the associated haplotype window 19743 and 19767 has no effect on yield, maturity, height or lodging.
  • Haplotype association information is provided in Table 2 where GM_A92205 indicates event MON 87708.
  • the DNA inserted into the genome of soybean plant MON 87708 and the flanking sequence was characterized by detailed molecular analyses. These analyses included: the insert sequence, the insert number (number of integration sites within the soybean genome), the copy number (number of copies of transgene DNA within one locus), the integrity of the inserted gene cassette, the flanking sequences, and the association of the insertion with haplotype regions of the soybean genome.
  • Molecular DNA probes were used that included the intact coding region and its respective regulatory elements, the promoters, introns, and polyadenylation sequences of the plant expression cassettes. The analysis showed that MON 87708 contains a single transgene DNA insertion with one copy of the expression cassette. Inverse PCR and DNA sequence analyses were performed to determine the 5' and 3 ' insert-to-plant genome junctions, confirm the organization of the elements within the insert ( Figure 1), and determine the complete DNA sequence of the insert in soybean plant MON 87708 (provided herein as SEQ ID NO: 5). A soybean plant that comprises in its genome the linked transgene genetic elements shown in Figure 1 and is resistant to dicamba is an aspect of the invention.
  • Sequences flanking the transgene DNA insertion in MON 87708 were determined using inverse PCR as described in Ochman et ah, 1990 (PCR Protocols: A guide to Methods and Applications, Academic Press, Inc.) and/or genome walker techniques. Plant genomic DNA was isolated from both A3525 and the transgenic soybean lines from tissue grown under standard greenhouse conditions. Approximately 1 gram of young leaf tissue was combined with liquid nitrogen and ground to a fine powder using a mortar and pestle. DNA was extracted using a NucleonTM PhytoPureTM Genomic DNA extraction kit (RPN8511, Amersham, Piscataway, NJ) according to the manufacturer's protocol.
  • RPN8511 NucleonTM PhytoPureTM Genomic DNA extraction kit
  • DNA was resuspended in 0.5 ml of TE (lOmM Tris-HCl pH 8.0, ImM EDTA).
  • TE lOmM Tris-HCl pH 8.0, ImM EDTA
  • This method can be modified by one skilled in the art to extract DNA from any tissue of soybean, including, but not limited to seed.
  • An aliquot of DNA was digested with restriction endonucleases selected based upon restriction analysis of the transgene DNA. After self-ligation of restriction fragments, PCR was performed using primers designed from the transgene DNA sequence that would amplify sequences extending away from the 5' and 3 ' ends of the transgene DNA.
  • PCR products were separated by agarose gel electrophoresis and purified using a QIAGEN gel purification kit (Qiagen, Valencia, CA). The subsequent DNA products were sequenced directly using standard DNA sequencing protocols.
  • the 5' flanking sequence which extends into the right border sequence of the expression cassette transgene DNA is presented as SEQ ID NO: 3 ([C], see Figure 1).
  • the 3 ' flanking sequence which extends into the left border sequence of the expression cassette transgene DNA is presented as SEQ ID NO: 4 ([D], see Figure 1).
  • SEQ ID NO: 5 [E], see Figure 1).
  • Isolated DNA molecule sequences were compared to the transgene DNA sequence to identify the flanking sequence and the co-isolated transgene DNA fragment. Confirmation of the presence of the expression cassette was achieved by PCR with primers designed based upon the deduced flanking sequence data and the known transgene DNA sequence. The wild type sequence corresponding to the same region in which the transgene DNA was integrated in the transformed line was isolated using primers designed from the flanking sequences in MON 87708. The PCR reactions were performed using the Elongase® amplification system (Invitrogen, Carlsbad, CA). The flanking DNA sequences in MON 87708 and the A3525 wild type sequence were analyzed against multiple nucleotide and protein databases.
  • flanking sequence and wild type sequences were used to design primers for TAQMAN® endpoint assays used to identify the events. Zygosity assays were developed using this information.
  • Example 3 Event specific endpoint TAQMAN® assays.
  • This example describes an event specific endpoint TAQMAN® thermal amplification method developed to identify event MON 87708 in a sample.
  • Examples of conditions useful with the event MON 87708 Specific Endpoint TAQMAN® method are as follows: Step 1: 18 megohm water adjusted for final volume of 10 ⁇ . Step 2: 5.0 ⁇ of 2X Universal Master Mix (dNTPs, enzyme, buffer) to a IX final concentration.
  • Step 3 0.5 ⁇ Event Primer-1 (SQ13570) and Event Primer-2 (SQ13571) Mix (resuspended in 18 megohm water to a concentration of 20 uM for each primer) to 1.0 ⁇ final concentration (for example in a microcentrifuge tube, the following should be added to achieve 500 ⁇ at a final concentration of 20uM: 100 ⁇ of Primer SQ13570 (SEQ ID NO: 9) at a concentration of 100 ⁇ ; 100 ⁇ of Primer SQ13571 (SEQ ID NO: 10) at a concentration of 100 ⁇ ; 300 ⁇ of 18 megohm water).
  • Step 4 0.2 ⁇ Event 6-FAMTM MGB Probe PB4655 (resuspended in 18 megohm water to a concentration of 10 ⁇ (SEQ ID NO: 11) to 0.2 ⁇ final concentration.
  • Step 5 0.5 ⁇ Internal Control Primer-1 and Internal Control Primer-2 Mix (resuspended in 18 megohm water to a concentration of 20 ⁇ for each primer) to 1.0 ⁇ final concentration.
  • Step 6 0.2 ⁇ Internal Control VICTM Probe to 0.2 ⁇ final concentration (resuspended in 18 megohm water to a concentration of 10 ⁇ )
  • Step 7 3.0 ⁇ Extracted DNA (template) for each sample with one each of the following comprising 1.
  • Negative control non-transgenic DNA
  • Negative water control no template
  • Positive control MON 87708 DNA.
  • Step 8 Thermocycler Conditions as follows: One Cycle at 50°C for 2 minutes; One Cycle at 95°C forlO minutes; Ten Cycles of 95°C for 15 seconds then 64°C for 1 minute with -l°C/cycle; Thirty Cycles of 95°C for 15 seconds then 54°C 1 minute; final cycle of 10°C.
  • the DNA primers used in the endpoint assay are primers SQ13570 (SEQ ID NO: 9), SQ13571 (SEQ ID NO: 10), and 6-FAMTM labeled probe PB4655 (SEQ ID NO: 11).
  • 6-FAM TM is a fluorescent dye product of Applied Biosystems (Foster City, CA) attached to the DNA probe.
  • TAQMAN® MGB TM probes the 5'exonuclease activity of Taq DNA polymerase cleaves the probe from the 5'-end, between the fluorophore and quencher. When hybridized to the target DNA strand, quencher and fluorophore are separated enough to produce a fluorescent signal, thus releasing fluorescence.
  • SQ13570 SEQ ID NO: 9
  • SQ13571 SEQ ID NO: 10 when used with these reaction methods with PB4655 (SEQ ID NO: 11) produce a DNA amplicon that is diagnostic for event MON 87708 DNA.
  • the controls for this analysis should include a positive control from soybean containing event MON 87708 DNA, a negative control from non-transgenic soybean, and a negative control that contains no template DNA.
  • a control for the PCR reaction includes Internal Control Primers and an Internal Control Probe, specific to a single copy gene in the Glycine genome.
  • Internal Control Primers and an Internal Control Probe, specific to a single copy gene in the Glycine genome.
  • primers specific to a single copy gene in the Glycine genome.
  • These assays are optimized for use with either an Applied Biosystems GeneAmp® PCR System 9700 (run at maximum speed) or MJ Research DNA Engine PTC- 225 thermal cycler. Other methods and apparatus known to those skilled in the art that produce amplicons that identify the event MON 87708 DNA is within the skill of the art.
  • R0 plants demonstrating the presence of the expression cassette were allowed to develop into fully mature plants. Probes designed based on the sequences of the dicamba tolerance transgene cassette were used to probe Southern blots to determine linkage. The R0 plants were also evaluated for copy number of the expression cassette using a combination of Southern analysis and endpoint TAQMAN®.
  • a zygosity assay is useful for determining if a plant comprising an event is homozygous for the event DNA; that is comprising the exogenous DNA in the same location on each chromosome of a chromosomal pair; or heterozygous for an event DNA, that is comprising the exogenous DNA on only one chromosome of a chromosomal pair; or is null for the event DNA, that is wildtype.
  • the endpoint TAQMAN® thermal amplification method was also used to develop zygosity assays for event MON 87708. This example describes an event specific endpoint TAQMAN® thermal amplification method developed to determine the zygosity of event MON 87708 in a sample.
  • primer SQ20632 hybridizes and extends specifically from the 3' junction of the inserted exogenous DNA and genomic DNA
  • primer SQ20636 (SEQ ID NO: 13) hybridizes and extends specifically from the DNA flanking the 3' side of the inserted exogenous DNA
  • primer SQ20637 (SEQ ID NO: 14) hybridizes and extends specifically from genomic DNA into which was integrated the inserted exogenous DNA.
  • the three primers are diagnostic for the event.
  • primer SQ20636 (SEQ ID NO: 13) and primer SQ20632 (SEQ ID NO: 12) and the 6-FAMTM-labeled oligonucleotide probe PB10130 (SEQ ID NO: 15) are diagnostic when there is a copy of the inserted exogenous DNA.
  • SQ20636 (SEQ ID NO: 13) and primer SQ20637 (SEQ ID NO: 14) and the VICTM-labeled oligonucleotide probe PB10131 (SEQ ID NO: 16) are diagnostic when there is no copy of the inserted exogenous DNA present in the genomic DNA, i.e. wildtype.
  • Step 1 18 megohm water adjusted for final volume of 10 ⁇ .
  • Step 2 5.0 ⁇ of 2X Universal Master Mix (Applied Biosystems cat # 4304437; dNTPs, enzyme, buffer) to a IX final concentration.
  • Step 3 0.5 ⁇ of Zygosity Primers SQ20632, SQ20636, SQ20637 (resuspended in 18 megohm water to a concentration of 20 ⁇ for each primer) to a final concentration of 1.0 ⁇ .
  • Step 4 0.2 ⁇ 6-FAMTM Probe PB 10130 (SEQ ID NO: 15) (resuspended in 18 megohm water to a concentration of 10 ⁇ ) to 0.2 ⁇ final concentration.
  • Step 5 0.2 ⁇ VICTM Probe PB10131 (SEQ ID NO: 16) (resuspended in 18 megohm water to a concentration of 10 ⁇ ) to 0.2 ⁇ final concentration.
  • Step 6 3.0 ⁇ Extracted DNA (template) for each sample with one each of the following comprising 1.
  • Leaf Samples to be analyzed (4-80 ng of genomic DNA diluted in water); 2.
  • Negative control non-transgenic soybean DNA; 4ng diluted in water); 3.
  • Negative water control no template; solution in which DNA was resuspended; 4.
  • Positive control MON 87708 genomic DNA from known heterozygous event (4 ng diluted in water); 5.
  • Positive control MON 87708 genomic DNA from known homozygous event (4 ng diluted in water).
  • Step 7 Gently mix.
  • Step 8 Thermocycler Conditions when using Applied Biosystems GeneAmp® PCR System 9700 (run at maximum speed) or MJ Research DNA Engine PTC-225 thermal cycler are as follows: One Cycle at 50°C for 2 minutes; one cycle at 95°C for 10 minutes; Ten Cycles of (95°C for 15 seconds then 64°C for 1 minute (-l°C/cycle); Thirty Cycles of (95°C for 15 seconds then 54°C for 1 minute); Optional additional 10 to 20 cycles (95°C for 15 seconds then 64°C for 1 minute (-l°C/cycle) may provide more distinct population separation during EndPoint TaqMan ® analysis; One cycle at 10°C hold.
  • Example 4 Identification of event MON 87708 in any MON 87708 breeding activity
  • the following example describes how one may identify the MON 87708 event within progeny of any breeding activity using soybean event MON 87708.
  • DNA event primer pairs are used to produce an amplicon diagnostic for soybean event MON 87708.
  • An amplicon diagnostic for MON 87708 comprises at least one junction sequence, provided as SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 7 or SEQ ID NO: 8.
  • Event primer pairs that will produce a diagnostic amplicon for MON 87708 include primer pairs based upon the flanking sequences and the inserted expression cassette.
  • SEQ ID NO: 1 To acquire a diagnostic amplicon in which SEQ ID NO: 1 is found, one would design a forward primer molecule based upon SEQ ID NO: 3 from bases 1 through 1126 and a reverse primer molecule based upon the inserted expression cassette DNA sequence (SEQ ID NO: 5 from positions 1 through 3003) in which the primer molecules are of sufficient length of contiguous nucleotides to specifically hybridize to SEQ ID NO: 3 and SEQ ID NO: 5.
  • a forward primer molecule based upon the inserted expression cassette DNA sequence (SEQ ID NO: 5 from positions 1 through 3003) and a reverse primer molecule based upon the 3 ' flanking sequence (SEQ ID NO: 4 from bases 131 through 1947), in which the primer molecules are of sufficient length of contiguous nucleotides to specifically hybridize to SEQ ID NO: 4 and SEQ ID NO: 5.
  • primers which produce amplicons of a limited size range, for example, between 100 to 1000 bases.
  • Smaller (shorter polynucleotide length) sized amplicons in general are more reliably produced in PCR reactions, allow for shorter cycle times, and can be easily separated and visualized on agarose gels or adapted for use in endpoint TAQMAN®-like assays.
  • Smaller amplicons can be produced and detected by methods known in the art of DNA amplicon detection.
  • amplicons produced using the primer pairs can be cloned into vectors, propagated, isolated, and sequenced or can be sequenced directly with methods well established in the art.
  • Any primer pair derived from the combination of SEQ ID NO: 3 and SEQ ID NO: 5 or the combination of SEQ ID NO: 4 and SEQ ID NO: 5 that are useful in a DNA amplification method to produce an amplicon diagnostic for MON 87708 or progeny thereof is an aspect of the invention.
  • Any single isolated DNA polynucleotide primer molecule comprising at least 11 contiguous nucleotides of SEQ ID NO: 3, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for MON 87708 or progeny thereof is an aspect of the invention.
  • Any single isolated DNA polynucleotide primer molecule comprising at least 11 contiguous nucleotides of SEQ ID NO: 4, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for MON 87708 or progeny thereof is an aspect of the invention.
  • Any single isolated DNA polynucleotide primer molecule comprising at least 11 contiguous nucleotides of SEQ ID NO: 5, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for MON 87708 or progeny thereof is an aspect of the invention.
  • a diagnostic amplicon comprises a DNA molecule homologous or complementary to at least one transgene/genomic junction DNA (SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 7 or SEQ ID NO: 8), or a substantial portion thereof.
  • An analysis for event MON 87708 plant tissue sample should include a positive tissue control from event MON 87708, a negative control from a soybean plant that is not event MON 87708 (for example, but not limited to A3525), and a negative control that contains no soybean genomic DNA.
  • a primer pair that will amplify an endogenous soybean DNA molecule will serve as an internal control for the DNA amplification conditions. Additional primer sequences can be selected from SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5 by those skilled in the art of DNA amplification methods, and conditions selected for the production of an amplicon by the methods shown in Example 3 may differ, but result in an amplicon diagnostic for event MON 87708 DNA.
  • the use of these DNA primer sequences with modifications to the methods of Example 3 are within the scope of the invention.
  • the amplicon produced by at least one DNA primer sequence derived from SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5 that is diagnostic for MON 87708 is an aspect of the invention.
  • DNA detection kits contain at least one DNA primer of sufficient length of contiguous nucleotides derived from SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5, that when used in a DNA amplification method produces a diagnostic amplicon for MON 87708 or its progeny is an aspect of the invention.
  • a MON 87708 soybean plant, plant part, plant cell, seed, or commodity product that will produce an amplicon diagnostic for MON 87708 when tested in a DNA amplification method is an aspect of the invention.
  • the assay for the MON 87708 amplicon can be performed by using an Applied Biosystems GeneAmp® PCR System 9700 (run at maximum speed) or MJ Research DNA Engine PTC-225 thermal cycler or any other amplification system that can be used to produce an amplicon diagnostic of MON 87708 as shown in Example 3.
  • a deposit of a representative sample of soybean event MON 87708 seed disclosed above and recited in the claims has been made under the Budapest Treaty with the American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA. 20110.
  • the ATCC accession number for this deposit is PTA-9670.
  • the deposit will be maintained in the depository for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced as necessary during that period.

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Abstract

The invention provides a transgenic soybean event MON 87708 plant and plants, plant cells, seeds, plant parts, and commodity products derived from event MON 87708. The invention also provides polynucleotides specific for event MON 87708 and plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides specific for event MON 87708. The invention also provides methods related to event MON 87708.

Description

SOYBEAN TRANSGENIC EVENT MON 87708 AND METHODS OF USE THEREOF
CROSS REFERENCE TO RELATED APPLICATIONS
This application claims the benefit of United States Provisional Application No. 61/243,227 filed September 17, 2009, which is herein incorporated by reference in its entirety. INCORPORATION OF SEQUENCE LISTING
The sequence listing that is contained in the file named "55544-0001_seqlisting.txt", which is 19.5 kilobytes (size as measured in Microsoft Windows®) and was created on August 13, 2010, is filed herewith by electronic submission and is incorporated by reference herein.
FIELD OF THE INVENTION
The invention relates to transgenic Glycine max event MON 87708. The event exhibits tolerance to dicamba herbicide. The invention also relates to plants, plant parts, plant seeds, plant cells, agricultural products, and methods related to event MON 87708 and provides nucleotide molecules that are unique to the event and were created in connection with the insertion of transgenic DNA into the genome of a Glycine max plant. BACKGROUND OF THE INVENTION
Soybean {Glycine max) is an important crop in many areas of the world, and the methods of biotechnology have been applied to this crop in order to produce soybean with desirable traits. One such desirable trait is herbicide tolerance. The expression of an herbicide tolerance transgene in a plant can confer the desirable trait of herbicide tolerance on the plant, but expression of the transgene may be influenced by the chromosomal location and the genomic result of the transgene insertion. For example, it has been observed in plants that there often is variation in the level and pattern of transgene expression among individual events that differ in the chromosomal insertion site of the transgene but are otherwise identical. There may also be undesirable and/or desirable phenotypic or agronomic differences between events. Because of this, it is often necessary to produce and analyze a large number of individual plant transformation events in order to select an event having both the desirable trait and the optimal phenotypic and agricultural characteristics necessary to make it suitable for commercial purposes. Such selection often requires greenhouse and field trials with many events over multiple years, in multiple locations, and under a variety of conditions so that a significant amount of agronomic, phenotypic, and molecular data may be collected. The resulting data and observations must then be analyzed by teams of scientists and agronomists with the goal of selecting a commercially suitable event. Such an event, once selected, may then be used for introgressing the desirable trait into other genetic backgrounds using plant breeding methods, and thus producing a number of different crop varieties that contain the desirable trait and are suitably adapted to specific local growing conditions.
SUMMARY OF THE INVENTION
The invention provides transgenic soybean plants designated event MON 87708, which exhibit commercially acceptable tolerance to applications of dicamba herbicide, having representative seed deposited with American Type Culture Collection (ATCC) with Accession No. PTA-9670. The invention also provides novel DNA molecules related to soybean event MON 87708 and methods of using these molecules. The invention also provides seeds, progeny, plant parts, cells, and commodity products of soybean event MON 87708. The invention also provides methods of using soybean event MON 87708 and methods of producing dicamba tolerant soybean.
The invention provides recombinant DNA molecules related to soybean event MON 87708. These recombinant DNA molecules may comprise nucleotide molecules having a nucleotide sequence representing a region of the genomic DNA flanking the transgene insertion, and/or a region of the transgene insertion, and/or a contiguous sequence of any of these regions such as a region of the junction between the transgene insertion and flanking genomic DNA of soybean event MON 87708. The invention also provides DNA molecules useful as primers and probes diagnostic for soybean event MON 87708 and amplicons diagnostic for the presence of soybean event MON 87708. Soybean plants, plant cells, plant parts, commodity products, progeny, and seeds comprising these molecules are also disclosed. The invention provides methods, compositions, and kits useful for detecting the presence and/or absence of DNA derived from soybean event MON 87708 and thus the presence and/or absence of the event. The invention provides a method for detection of MON 87708 by contacting a sample comprising DNA with a primer set that when used in a nucleic acid amplification reaction with genomic DNA from soybean event MON 87708 produces an amplified DNA diagnostic for soybean event MON 87708, performing a nucleic acid amplification reaction thereby producing the amplified DNA, and detecting the presence and/or absence of the amplified DNA. The invention also provides a method for detection of MON 87708 by contacting a sample comprising DNA with a probe that when used in a hybridization reaction with DNA from soybean event MON 87708 hybridizes to a DNA molecule specific for soybean event MON 87708, performing a hybridization reaction, and detecting the hybridization of the probe to the DNA molecule. Kits comprising the methods and compositions of the invention useful for detecting the presence of DNA derived from soybean event MON 87708 are also provided.
The invention provides a soybean plant, seed, plant cell, progeny plant, plant part, or commodity product derived from a plant, plant cell, or seed of soybean event MON 87708. The invention also provides a soybean plant, seed, plant cell, progeny plant, plant part, or commodity product comprising a recombinant DNA molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8, and complements and fragments thereof. The invention also provides a soybean plant, seed, plant cell, progeny plant, plant part, or commodity product derived from the plant or seed of soybean event MON 87708 and comprising a recombinant DNA molecule that produces an amplified DNA molecule comprising SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8 in a DNA amplification method.
The invention provides a method for controlling weeds in a field by planting soybean event MON 87708 and then applying an effective dose of dicamba herbicide capable of controlling the weeds without injuring the soybean event MON 87708 plants. The invention also provides a method for controlling weeds in a field by applying an effective dose of dicamba herbicide to control weeds in a field and then planting soybean event MON 87708 in the field. The invention also provides a method for producing soybean seed essentially free of the seeds of toxic weed species by planting seeds of a dicamba tolerant soybean variety MON 87708 in a field, applying a post-emergence effective dose of dicamba herbicide sufficient to kill the toxic weed species to the field, and harvesting seed from the field.
The invention provides methods of producing a soybean plant and/or seed that tolerates application of dicamba herbicide by sexually crossing a soybean event MON 87708 plant comprising SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8 with a second soybean plant, thereby producing seed, growing the seed to produce progeny plants, treating the progeny plants with dicamba, and selecting a progeny plant that is tolerant to dicamba. The methods may also include selfing the selected progeny plant to produce a plurality of second generation progeny plants and selecting from these a dicamba tolerant plant. The methods may also include sexually crossing the selected progeny plant with another soybean plant to produce seed, growing the seed to produce a second generation of progeny plants, treating the second generation of progeny plants with dicamba, and selecting a second generation progeny plant that is tolerant to dicamba. The invention provides methods of producing a soybean plant and/or seed that tolerates application of dicamba herbicide by selfing a dicamba tolerant soybean event MON 87708 plant comprising SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8, thereby producing seed, growing the seed to produce progeny plants, treating the progeny plants with dicamba; and selecting a progeny plant that is tolerant to dicamba.
The invention provides methods of determining the zygosity of a soybean event MON 87708 plant or seed comprising contacting a soybean DNA sample with a primer set comprising SEQ ID NO: 12, SEQ ID NO: 13, and SEQ ID NO: 14 and a probe set comprising SEQ ID NO: 15 and SEQ ID NO: 16; then performing a nucleic acid amplification reaction with the sample, primer set, and probe set; then detecting in then nucleic acid amplification reaction a first fluorescent signal that is diagnostic for event MON 87708 and a second fluorescent signal different from the first fluorescent signal and that is diagnostic for native soybean genomic DNA corresponding to the location of insertion of the event MON 87708 transgene; and analyzing the presence and/or absence of the first fluorescent signal and the second fluorescent signal in the nucleic acid amplification reaction, wherein the presence of both fluorescent signals indicates the sample is heterozygous for event MON 87708 and the presence of only the first fluorescent signal indicates the sample is homozygous for event MON 87708. The invention also provides a soybean plant, seed, plant cell, or plant part comprising soybean haplotype region on linkage group 9 at approximately map position 143.5 comprising a dicamba tolerance gene and further defined by haplotype window 19743 and 19767, and methods of using the same. The foregoing and other aspects of the invention will become more apparent from the following detailed description.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 illustrates the organization of the transgenic insert in the genome of soybean event MON 87708; [A] corresponds to the relative position of SEQ ID NO: 1, which is sixty nucleotides of the junction between the soybean genomic DNA and the 5' portion of the transgene insert DNA; [Α'] corresponds to the relative position of SEQ ID NO: 7, which is one hundred nucleotides of the junction between the soybean genomic DNA and the 5' portion of the transgene insert DNA; [B] corresponds to the relative position of SEQ ID NO: 2, which is sixty nucleotides of the junction between the soybean genomic DNA and the 3' portion of the transgene insert DNA; [Β'] corresponds to the relative position of SEQ ID NO: 8, which is one hundred nucleotides of the junction between the soybean genomic DNA and the 3 ' portion of the transgene insert DNA; [C] corresponds to the relative position of SEQ ID NO: 3, which is the soybean genome sequence flanking the arbitrarily assigned/designated 5' end of the expression cassette integrated into the genome in event MON 87708; [D] corresponds to the relative position of SEQ ID NO: 4, which is the soybean genome sequence flanking the arbitrarily assigned/designated 3' end of the expression cassette integrated into the genome in event MON 87708; [E] represents the various elements comprising SEQ ID NO: 5 and is the sequence of the expression cassette inserted into the genome of the event MON 87708; and [F] represents the contiguous sequence (provided as SEQ ID NO: 6) comprising, as represented in the figure from left to right, SEQ ID NO: 3, SEQ ID NO: 5 and SEQ ID NO: 4, in which SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and SEQ ID NO: 8 are included, as these sequences are present in the genome in event MON 87708. BRIEF DESCRIPTION OF THE SEQUENCES
SEQ ID NO: 1 is a sixty nucleotide sequence representing the 5' junction between the soybean genomic DNA and the integrated transgenic expression cassette. SEQ ID NO: 1 is positioned in SEQ ID NO: 6 at nucleotide position 1097-1156.
SEQ ID NO: 2 is a sixty nucleotide sequence representing the 3 ' junction between the soybean genomic DNA and the integrated transgenic expression cassette. SEQ ID NO: 2 is positioned in SEQ ID NO: 6 at nucleotide position 4100-4159.
SEQ ID NO: 3 is the 5' sequence flanking the inserted DNA of soybean event MON 87708 up to and including a region of transgene DNA insertion.
SEQ ID NO: 4 is the 3 ' sequence flanking the inserted DNA of soybean event MON 87708 up to and including a region of transgene DNA insertion.
SEQ ID NO: 5 is the sequence of the integrated transgenic expression cassette.
SEQ ID NO: 6 is the nucleotide sequence representing the contig of the 5' sequence flanking the inserted DNA of soybean event MON 87708 (SEQ ID NO: 3), the sequence of the inserted DNA (SEQ ID NO: 5), and the 3' sequence flanking the inserted DNA of soybean event MON 87708 (SEQ ID NO: 4) and includes SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and SEQ ID NO: 8.
SEQ ID NO: 7 is a one hundred nucleotide sequence representing the 5' junction between the soybean genomic DNA and the integrated transgenic expression cassette.
SEQ ID NO: 8 is a one hundred nucleotide sequence representing the 3' junction between the soybean genomic DNA and the integrated transgenic expression cassette.
SEQ ID NO: 9 is the sequence of a primer referred to as Primer SQ13570 and used to identify soybean event MON 87708. It is complimentary to the inserted expression cassette at the region close to the 3' transgene insertion border. A PCR amplicon produced from a TAQMAN® (PE Applied Biosystems, Foster City, CA) assay using the combination of primers SQ13570 and SQ13571 (SEQ ID NO: 10) is a positive result for the presence of the event MON 87708.
SEQ ID NO: 10 is the sequence of a primer referred to as Primer SQ13571 and used to identify soybean event MON 87708. It is complimentary to a 3 'region flanking the inserted expression cassette and close to the transgene DNA insertion border. A PCR amplicon produced from a TAQMAN® (PE Applied Biosystems, Foster City, CA) assay using the combination of primers SQ13570 (SEQ ID NO: 9) and SQ13571 is a positive result for the presence of the event MON 87708.
SEQ ID NO: 11 is the sequence of a probe referred to as Probe PB4655 and used to identify soybean event MON 87708. It is complimentary to a region spanning the 3' junction of the inserted expression cassette and the genomic DNA. This probe is a 6-FAM™-labeled synthetic oligonucleotide. Release of a fluorescent signal in an amplification reaction using primers SQ13570 and SQ13571 (SEQ ID NO: 9-10) in combination with 6-FAM™-labeled probe PB4655 is diagnostic of event MON 87708 in a TAQMAN® assay.
SEQ ID NO: 12 is the sequence of a primer referred to as Primer SQ20632 and used to identify MON 87708 event zygosity.
SEQ ID NO: 13 is the sequence of a primer referred to as Primer SQ20636 and used to identify soybean wild-type and MON 87708 event zygosity.
SEQ ID NO: 14 is the sequence of a primer referred to as Primer SQ20637 and used to identify soybean wild-type zygosity.
SEQ ID NO: 15 is the sequence of a probe referred to as Probe PB10130 and used for a
MON 87708 event zygosity assay.
SEQ ID NO: 16 is the sequence of a probe referred to as Probe PB10131 and used for a soybean wild-type zygosity assay.
DETAILED DESCRIPTION
The following definitions and methods are provided to better define the invention and to guide those of ordinary skill in the art in the practice of the invention. Unless otherwise noted, terms are to be understood according to conventional usage by those of ordinary skill in the relevant art.
The invention provides a transgenic soybean event MON 87708 that exhibits commercially acceptable tolerance to applications of dicamba herbicide. The event comprises a single insertion of transgenic DNA into the chromosome/genome of the soybean germplasm. An "event" is produced by: (i) transformation of a plant cell with a nucleic acid construct that includes a transgene of interest, (ii) regeneration of a population of plants resulting from the insertion of the transgene into the genome of the plant, and (iii) selection of a particular plant characterized by insertion of the transgene into a particular location in the plant's genome. The term "event" refers to the original transformant that includes the transgene inserted into the particular location in the plant's genome. The term "event" also refers to progeny of the transformant that include the transgene inserted into the particular location in the plant's genome. Such progeny may be produced by a sexual outcross between the transformant, or its progeny, and another plant. Such other plant may be a transgenic plant comprising the same or different transgene and/or a nontransgenic plant, such as one from a different variety. Even after repeated back-crossing to a recurrent parent, the inserted DNA and flanking DNA from the transformed parent is present in the progeny of the cross at the same genomic location.
As used herein, the term "soybean" means Glycine max and includes all plant varieties that can be bred with soybean, including wild soybean species as well as those plants belonging to Glycine that permit breeding between species.
The term "event" also refers to a DNA molecule from the original transformant comprising the inserted DNA and the flanking soybean genomic DNA immediately adjacent to either side of the inserted DNA. This DNA molecule is created by the act of inserting the transgenic DNA into the genome of the soybean plant, i.e. , by the act of transformation. This DNA molecule therefore comprises a nucleotide sequence that is both specific to the event and that is unique to the genome of the soybean plant into which the transgenic DNA has been inserted, in that this nucleotide sequence contains both the sequence of a particular region of soybean genomic DNA and of the transgenic DNA insert. The arrangement of the inserted DNA in soybean event MON 87708 in relation to the surrounding soybean plant genome DNA is therefore specific and unique for soybean event MON 87708. This DNA molecule is also an integral part of the soybean chromosome of event MON 87708 and as such is static in the plant and may be passed on to progeny of the plant.
Event MON 87708 comprises a transgene that confers tolerance to applications of dicamba herbicide to the soybean plant. "Dicamba" refers to 3,6-dichloro-2-methoxybenzoic acid. Dicamba is a synthetic auxin herbicide useful for controlling broadleaf weeds. Soybean plants were transformed with dicamba mono-oxygenase (DMO), an enzyme cloned from Stenotrophomonas maltophilia which is commonly found in soil rhizosphere. Dicamba mono-oxygenase is an enzyme that catalyzes the deactivation of dicamba via an O-demethylation reaction to the nonherbicidal compound 3,5- dichlorosalicylic acid. In some areas of the world toxic weed species seeds may contaminate harvested soybean seeds that can affect the health and nutrition of animals fed the contaminated soybean commodity products. These plants can be eliminated from a soybean field by treatment with a dicamba herbicide. Members of this group of toxic weeds include Cardaria spp, Heliotropium spp, Centaurea spp., Senecio spp., Crotalaria spp., Solanum spp., Xanthium spp., Amsinckia spp., Cassia spp., Sesbania spp., Datura spp., Ricinus spp., Argemone spp., Corchorus spp., Impomoea spp., and Echium spp.
As used herein, the term "recombinant" refers to a form of DNA and/or protein and/or an organism that would not normally be found in nature and as such was created by human intervention. Such human intervention may produce a recombinant DNA molecule and/or a recombinant plant. As used herein, a "recombinant DNA molecule" is a DNA molecule comprising a combination of DNA molecules that would not naturally occur together and is the result of human intervention, e.g., a DNA molecule that is comprised of a combination of at least two DNA molecules heterologous to each other, and/or a DNA molecule that is artificially synthesized and comprises a polynucleotide sequence that deviates from the polynucleotide sequence that would normally exist in nature, and/or a DNA molecule that comprises a transgene artificially incorporated into a host cell's genomic DNA and the associated flanking DNA of the host cell's genome. An example of a recombinant DNA molecule is a DNA molecule described herein resulting from the insertion of the transgene into the soybean genomic DNA, which may ultimately result in the expression of a recombinant RNA and/or protein molecule in that organism. As used herein, a "recombinant plant" is a plant that would not normally exist in nature, is the result of human intervention, and contains a transgene and/or heterologous DNA molecule incorporated into its genome. As a result of such genomic alteration, the recombinant plant is distinctly different from the related wildtype plant. An example of a recombinant plant is a soybean plant described herein as Event MON 87708.
As used herein, the term "transgene" refers to a nucleotide molecule artificially incorporated into a host cell's genome. Such transgene may be heterologous to the host cell. The term "transgenic plant" refers to a plant comprising such a transgene.
As used herein, the term "heterologous" refers to a first molecule not normally found in combination with a second molecule in nature. For example, a molecule may be derived from a first species and inserted into the genome of a second species. The molecule would thus be heterologous to the host and artificially incorporated into a host cell's genome.
As used herein, the term "chimeric" refers to a single DNA molecule produced by fusing a first DNA molecule to a second DNA molecule, where neither first nor second DNA molecule would normally be found in that configuration, i.e., fused to the other. The chimeric DNA molecule is thus a new DNA molecule not otherwise normally found in nature.
The invention provides DNA molecules and their corresponding nucleotide sequences. As used herein, the term "DNA", "DNA molecule", "nucleotide molecule" refers to a DNA molecule of genomic or synthetic origin, i.e., a polymer of deoxyribonucleotide bases or a polynucleotide molecule, read from the 5' (upstream) end to the 3' (downstream) end. As used herein, the term "DNA sequence", "nucleotide sequence" or "polynucleotide sequence" refers to the nucleotide sequence of a DNA molecule. The nomenclature used herein is that required by Title 37 of the United States Code of Federal Regulations § 1.822 and set forth in the tables in WIPO Standard ST.25 (1998), Appendix 2, Tables 1 and 3. By convention, the nucleotide sequences of the invention provided as SEQ ID NO: 1-8 and fragments thereof are disclosed with reference to only one strand of the two complementary nucleotide sequence strands. By implication, the complementary sequences {i.e. the sequences of the complementary strand), also referred to in the art as the reverse complementary sequences, are within the scope of the invention and are expressly intended to be within the scope of the subject matter claimed.
The nucleotide sequence corresponding to the complete nucleotide sequence of the inserted transgenic DNA and substantial segments of the soybean genome DNA flanking either end of the inserted transgenic DNA is provided herein as SEQ ID NO: 6. A subsection of this is the inserted transgenic DNA provided as SEQ ID NO: 5. The nucleotide sequence of the soybean genome DNA physically linked by phosphodiester bond linkage to and therefore flanking the 5 ' end of the inserted transgenic DNA is set forth as shown in SEQ ID NO: 3. The nucleotide sequence of the soybean genome DNA physically linked by phosphodiester bond linkage to and therefore flanking the 3 ' end of the inserted transgenic DNA is set forth as shown in SEQ ID NO: 4.
The soybean event MON 87708 further comprises two regions, one spanning the 5' location and one spanning the 3 ' location where the transgenic DNA is inserted into the genomic DNA, referred to herein as the 5' and 3' junction, respectively. A "junction sequence" or "junction region" refers to the DNA sequence and/or corresponding DNA molecule that spans the inserted transgenic DNA and the adjacent flanking genomic DNA. The junction sequences may be arbitrarily represented by the two 60 nucleotide sequences provided as SEQ ID NO: 1 and SEQ ID NO: 2, each representing 30 nucleotides of the flanking genomic DNA adjacent to and contiguous with 30 nucleotides of insert DNA. Alternatively, the junction sequences may be arbitrarily represented by the two 100 nucleotide sequences provided as SEQ ID NO: 7 and SEQ ID NO: 8, each representing 50 nucleotides of the flanking genomic DNA adjacent to and contiguous with 50 nucleotides of insert DNA. These nucleotides are connected by phosphodiester linkage and in soybean event MON 87708 are present as part of the genome. In soybean the identification of one or more of SEQ ID NO: 1 , SEQ ID NO: 2, SEQ ID NO: 7, and SEQ ID NO: 8 in a sample derived from a soybean plant, seed, or plant part is determinative that the DNA was obtained from soybean event MON 87708 and is diagnostic for the presence in a sample of DNA from soybean event MON 87708. The invention thus provides a DNA molecule that contains at least the nucleotide sequence as set forth in SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8. Any segment of DNA derived from transgenic soybean event MON 87708 that is sufficient to include SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8 is within the scope of The invention. In addition, any polynucleotide comprising a sequence complementary to any of the sequences described within this paragraph is within the scope of the invention. Figure 1 illustrates the physical arrangement of SEQ ID NO: 1-5 and 7-8 relative to SEQ ID NO: 6 arranged from 5'to 3'.
The invention provides exemplary DNA molecules that can be used either as primers or probes for diagnosing the presence of DNA derived from soybean plant event MON 87708 in a sample. Such primers or probes are specific for a target nucleic acid sequence and as such are useful for the identification of soybean event MON 87708 nucleic acid sequence by the methods of the invention described herein.
A "primer" is typically a highly purified, isolated polynucleotide that is designed for use in specific annealing or hybridization methods that involve thermal amplification. A pair of primers may be used with template DNA, such as a sample of soybean genomic DNA, in a thermal amplification, such as polymerase chain reaction (PCR), to produce an amplicon, where the amplicon produced from such reaction would have a DNA sequence corresponding to sequence of the template DNA located between the two sites where the primers hybridized to the template. As used herein, an "amplicon" is a piece or fragment of DNA that has been synthesized using amplification techniques. An amplicon of the invention comprises at least SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8. A primer is typically designed to hybridize to a complementary target DNA strand to form a hybrid between the primer and the target DNA strand, and the presence of the primer is a point of recognition by a polymerase to begin extension of the primer (i.e., polymerization of additional nucleotides into a lengthening nucleotide molecule) using as a template the target DNA strand. Primer pairs, as used in the invention, are intended to refer to use of two primers binding opposite strands of a double stranded nucleotide segment for the purpose of amplifying linearly the polynucleotide segment between the positions targeted for binding by the individual members of the primer pair, typically in a thermal amplification reaction or other conventional nucleic-acid amplification methods. Exemplary DNA molecules useful as primers are provided as SEQ ID NO: 9-10. The primer pair provided as SEQ ID NO: 9 and SEQ ID NO: 10 are useful as a first DNA molecule and a second DNA molecule that is different from the first DNA molecule, and both are each of sufficient length of contiguous nucleotides of either SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6 to function as DNA primers that, when used together in a thermal amplification reaction with template DNA derived from soybean event MON 87708, produce an amplicon comprising SEQ ID NO: 2.
A "probe" is an isolated nucleic acid that is complementary to a strand of a target nucleic acid. Probes according to the invention include not only deoxyribonucleic or ribonucleic acids but also polyamides and other probe materials that bind specifically to a target DNA sequence and the detection of such binding can be useful in diagnosing, discriminating, determining, or confirming the presence of that target DNA sequence in a particular sample. A probe may be attached to a conventional detectable label or reporter molecule, e.g., a radioactive isotope, ligand, chemiluminescent agent, or enzyme. An exemplary DNA molecule useful as a probe is provided as SEQ ID NO: 11.
Probes and primers according to the invention may have complete sequence identity with the target sequence, although primers and probes differing from the target sequence that retain the ability to hybridize preferentially to target sequences may be designed by conventional methods. In order for a nucleic acid molecule to serve as a primer or probe it need only be sufficiently complementary in sequence to be able to form a stable double -stranded structure under the particular solvent and salt concentrations employed. Any conventional nucleic acid hybridization or amplification method can be used to identify the presence of transgenic DNA from soybean event MON 87708 in a sample. Probes and primers are generally at least about 11 nucleotides, at least about 18 nucleotides, at least about 24 nucleotides, or at least about 30 nucleotides or more in length. Such probes and primers hybridize specifically to a target DNA sequence under stringent hybridization conditions. Conventional stringency conditions are described by Sambrook et ah, 1989, and by Haymes et al , In: Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, DC (1985). As used herein, two nucleic acid molecules are capable of specifically hybridizing to one another if the two molecules are capable of forming an anti-parallel, double-stranded nucleic acid structure. A nucleic acid molecule is the "complement" of another nucleic acid molecule if they exhibit complete complementarity. As used herein, molecules exhibit "complete complementarity" when every nucleotide of one of the molecules is complementary to a nucleotide of the other. Two molecules are "minimally complementary" if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional "low- stringency" conditions. Similarly, the molecules are "complementary" if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional "high-stringency" conditions. Departures from complete complementarity are therefore permissible, as long as such departures do not completely preclude the capacity of the molecules to form a double-stranded structure.
As used herein, the term "isolated" refers to at least partially separating a molecule from other molecules normally associated with it in its native or natural state. In one embodiment, the term "isolated" refers to a DNA molecule that is at least partially separated from the nucleic acids which normally flank the DNA molecule in its native or natural state. Thus, DNA molecules fused to regulatory or coding sequences with which they are not normally associated, for example as the result of recombinant techniques, are considered isolated herein. Such molecules are considered isolated even when integrated into the chromosome of a host cell or present in a nucleic acid solution with other DNA molecules.
Any number of methods well known to those skilled in the art can be used to isolate and manipulate a DNA molecule, or fragment thereof, disclosed in the invention. For example, PCR (polymerase chain reaction) technology can be used to amplify a particular starting DNA molecule and/or to produce variants of the original molecule. DNA molecules, or fragment thereof, can also be obtained by other techniques such as by directly synthesizing the fragment by chemical means, as is commonly practiced by using an automated oligonucleotide synthesizer.
The DNA molecules and corresponding nucleotide sequences provided herein are therefore useful for, among other things, identifying soybean event MON 87708, selecting plant varieties or hybrids comprising soybean event MON 87708, detecting the presence of DNA derived from the transgenic soybean event MON 87708 in a sample, and monitoring samples for the presence and/or absence of soybean event MON 87708 or plant parts derived from soybean event MON 87708.
The invention provides soybean plants, progeny, seeds, plant cells, plant parts (such as pollen, ovule, pod, flower tissue, root tissue, stem tissue, and leaf tissue), and commodity products. These plants, progeny, seeds, plant cells, plant parts, and commodity products contain a detectable amount of a polynucleotide of the invention, i.e., such as a polynucleotide having at least one of the sequences provided as SEQ ID NO: 1-8. Plants, progeny, seeds, plant cells, and plant parts of the invention may also contain one or more additional transgenes. Such transgene may be any nucleotide sequence encoding a protein or RNA molecule conferring a desirable trait including but not limited to increased insect resistance, increased water use efficiency, increased yield performance, increased drought resistance, increased seed quality, improved nutritional quality, and/or increased herbicide tolerance, in which the desirable trait is measured with respect to a soybean plant lacking such additional transgene.
The invention provides soybean plants, progeny, seeds, plant cells, and plant part such as pollen, ovule, pod, flower, root or stem tissue, and leaves derived from a transgenic soybean plant event MON 87708. A representative sample of soybean event MON 87708 seed has been deposited according to the Budapest Treaty for the purpose of enabling the invention. The repository selected for receiving the deposit is the American Type Culture Collection (ATCC) having an address at 10801 University Boulevard, Manassas, Virginia USA, Zip Code 20110. The ATCC repository has assigned the accession No. PTA-9670 to the event MON 87708 seed.
The invention provides a microorganism comprising a DNA molecule having SEQ ID NO: 1 and SEQ ID NO: 2 present in its genome. An example of such a microorganism is a transgenic plant cell. Microorganisms, such as a plant cell of The invention, are useful in many industrial applications, including but not limited to: (i) use as research tool for scientific inquiry or industrial research; (ii) use in culture for producing endogenous or recombinant carbohydrate, lipid, nucleic acid, or protein products or small molecules that may be used for subsequent scientific research or as industrial products; and (iii) use with modern plant tissue culture techniques to produce transgenic plants or plant tissue cultures that may then be used for agricultural research or production. The production and use of microorganisms such as transgenic plant cells utilizes modern microbiological techniques and human intervention to produce a man-made, unique microorganism. In this process, recombinant DNA is inserted into a plant cell's genome to create a transgenic plant cell that is separate and unique from naturally occurring plant cells. This transgenic plant cell can then be cultured much like bacteria and yeast cells using modern microbiology techniques and may exist in an undifferentiated, unicellular state. The new plant cell's genetic composition and phenotype is a technical effect created by the integration of the heterologous DNA into the genome of the cell. Another aspect of the invention is a method of using a microorganism of the invention. Methods of using microorganisms of the invention, such as transgenic plant cells, include (i) methods of producing transgenic cells by integrating recombinant DNA into the genome of the cell and then using this cell to derive additional cells possessing the same heterologous DNA; (ii) methods of culturing cells that contain recombinant DNA using modern microbiology techniques; (iii) methods of producing and purifying endogenous or recombinant carbohydrate, lipid, nucleic acid, or protein products from cultured cells; and (iv) methods of using modern plant tissue culture techniques with transgenic plant cells to produce transgenic plants or transgenic plant tissue cultures.
Plants of the invention may pass along the event DNA, including the transgene, to progeny. As used herein, "progeny" includes any plant, seed, plant cell, and/or regenerable plant part comprising the event DNA derived from an ancestor plant and/or a polynucleotide having at least one of the sequences provided as SEQ ID NO: 1 and SEQ ID NO: 2. Plants, progeny, and seeds may be homozygous or heterozygous for the transgene. Progeny may be grown from seeds produced by a soybean event MON 87708 plant and/or from seeds produced by a plant fertilized with pollen from a soybean event MON 87708 plant. Progeny plants may be self-pollinated (also known as "selfing") to generate a true breeding line of plants, i.e., plants homozygous for the transgene. Selfing of appropriate progeny can produce plants that are homozygous for both added, exogenous genes.
Alternatively, progeny plants may be outcrossed, e.g., bred with another unrelated plant, to produce a varietal or a hybrid seed or plant. The other unrelated plant may be transgenic or nontransgenic. A varietal or hybrid seed or plant of the invention may thus be derived by crossing a first parent that lacks the specific and unique DNA of the soybean event MON 87708 with a second parent comprising soybean event MON 87708, resulting in a hybrid comprising the specific and unique DNA of the soybean event MON 87708. Each parent can be a hybrid or an inbred/varietal, so long as the cross or breeding results in a plant or seed of the invention, i.e., a seed having at least one allele containing the specific and unique DNA of soybean event MON 87708 and/or SEQ ID NO: 1 and SEQ ID NO: 2. Two different transgenic plants may thus be mated to produce hybrid offspring that contain two independently segregating, added, exogenous genes. For example, the MON 87708 dicamba tolerant soybean can be crossed with other transgenic soybean plant to produce a plant having the characteristics of both transgenic parents. One example of this would be a cross of MON 87708 dicamba tolerant soybean with a plant having one or more additional traits such as herbicide tolerance (e.g. , soybean event 40-3-2 or soybean event MON89788 (U.S. Patent Application Publication No. 20060282915)), insect control (e.g. soybean event MON87701 (U.S. Patent Application Publication No. 20090130071)), and/or other desirable traits (e.g. enhanced oil composition such as soybean event MON87769 (PCT Patent Publication WO2009102873)), resulting in a progeny plant or seed that is tolerant to dicamba and has one or more additional traits. Herbicides for which transgenic plant tolerance has been demonstrated and the method of the invention can be applied, include but are not limited to: glyphosate, glufosinate, sulfonylureas, imidazolinones, bromoxynil, delapon, cyclohexanedione, protoporphyrionogen oxidase inhibitors, and isoxasflutole herbicides. Nucleotide molecules encoding proteins involved in herbicide tolerance are known in the art and include, but are not limited to, a nucleotide molecule encoding: glyphosate-tolerant 5-enolpyruvylshikimate-3- phosphate synthase (EPSPS) (see, for example, U.S. Patent Nos. 5,627,061 ; 5,633,435; 6,040,497; 5,094,945; 5,804,425; 6,248,876; 7,183,110; RE39,247); glyphosate oxidoreductase (GOX) (see, for example, U.S. Patent No. 5,776,760); glyphosate-n-acetyltransferase (GAT); an herbicide-tolerant acetolactate synthase (ALS, also known as acetohydroxyacid synthase (AHAS)) for tolerance to sulfonylureas, imidazolinones, triazolopyrimidines, pyrimidinyl oxybenzoates, sulfonylamino carbonyl triazolinones, and/or heteroaryl ethers; an herbicide-tolerant acetyl coenzyme A carboxylase (ACCase) or R-2,4-dichlorophenoxypropionate dioxygenase (rdpA) for tolerance to an aryloxyphenoxypropionate (AOPP) (such as haloxyfop, quizalofop, dichlorofop, and diclofop); a detoxification protein such as a 2,4-D dioxygenase (tfdA), R-2,4-dichlorophenoxypropionate dioxygenase (rdpA), AryloxyAlkanoate Dioxygenase (AAD), and/or S-2,4-dichorprop dioxygenase (sdpA) for tolerance to synthetic auxin herbicides; a bromoxynil nitrilase (Bxn) for Bromoxynil tolerance {see, for example, U.S. Patent No. 4,810,648); a phytoene desaturase (crtl) for tolerance to norflurazon; the bialaphos resistance (bar) or phosphinothricin acetyltransferase (PAT) protein {see, for example, U.S. Patent No. 5,646,024 and 5,276,268) for tolerance to glufosinate and bialaphos; and a protein for triketone (mezotrione, tembotrione, topromezone, isoxazole) herbicide -tolerance such as tolerant 4-HydroxyPhenylPyruvate Dioxygenase (HPPD), a detoxifying cytochrome P450, or an HPPD pathway bypass such as Artbrobacter globiformis HPP oxidase (HPPO) and Pseudomonas acidovorans 4-HPA 1 -hydroxylase (HP AH) and NADH oxidoreductase (HP AC).
Back-crossing to a parental plant and out-crossing with a non-transgenic plant are also contemplated, as is vegetative propagation. Descriptions of other breeding methods that are commonly used for different traits and crops can be found in one of several references, e.g., Fehr, in Breeding Methods for Cultivar Development, Wilcox J. ed., American Society of Agronomy, Madison WI (1987).
The invention provides a plant part that is derived from soybean event MON 87708. As used herein, a "plant part" refers to any part of a plant which is comprised of material derived from a soybean event MON 87708 plant. Plant parts include but are not limited to pollen, ovule, pod, flower, root or stem tissue, fibers, and leaves. Plant parts may be viable, nonviable, regenerable, and/or nonregenerable.
The invention provides a commodity product that is derived from soybean event MON 87708. As used herein, a "commodity product" refers to any composition or product which is comprised of material derived from a soybean event MON 87708 plant, seed, plant cell, or plant part. Commodity products may be sold to consumers and may be viable or nonviable. Nonviable commodity products include but are not limited to nonviable seeds and grains; processed seeds, seed parts, and plant parts; dehydrated plant tissue, frozen plant tissue, and processed plant tissue; seeds and plant parts processed for animal feed for terrestrial and/or aquatic animals consumption, oil, meal, flour, flakes, bran, fiber, milk, cheese, paper, cream, wine, and any other food for human consumption; and biomasses and fuel products. Viable commodity products include but are not limited to seeds and plant cells. The soybean event MON 87708 can thus be used to manufacture any commodity product typically acquired from soybean. Any such commodity product that is derived from the soybean event MON 87708 may contain at least a detectable amount of the specific and unique DNA corresponding to soybean event MON 87708, and specifically may contain a detectable amount of a polynucleotide containing at least 15 contiguous nucleotides of SEQ ID NO: 1 or SEQ ID NO: 2. Any standard method of detection for nucleotide molecules may be used, including methods of detection disclosed herein. A commodity product is within the scope of the invention if there is any detectable amount of SEQ ID NO: 1 or SEQ ID NO: 2 in the commodity product.
The plants, progeny, seeds, plant cells, plant parts (such as pollen, ovule, pod, flower, root or stem tissue, and leaves), and commodity products of the invention are therefore useful for, among other things, growing plants for the purpose of producing seed and/or plant parts of soybean event MON 87708 for agricultural purposes, producing progeny of soybean event MON 87708 for plant breeding and research purposes, use with microbiological techniques for industrial and research applications, and sale to consumers.
The invention provides methods for controlling weeds and methods for producing plants using dicamba herbicide and soybean event MON 87708. A method for controlling weeds in a field is provided and consists of planting soybean event MON 87708 varietal or hybrid plants in a field and applying a herbicidally effective dose of dicamba to the field for the purpose of controlling weeds in the field without injuring the MON 87708 plants. Such application of dicamba herbicide may be pre -emergence, i.e. , any time after MON 87708 seed is planted and before MON 87708 plants emerge, or post-emergence, i.e., any time after MON 87708 plants emerge. Another method for controlling weeds in a field is also provided and consists of applying an effective dose of dicamba herbicide to control weeds in a field and then planting soybean event MON 87708 in the field. Such application of dicamba herbicide would be pre-planting, i.e., before MON 87708 seed is planted, and could be done any time pre-planting including, but not limited to, about 14 days pre-planting to about 1 day pre-planting. The invention also provides a method for producing soybean seed essentially free of the seeds of toxic weed species by planting seeds of a dicamba tolerant soybean variety MON 87708 in a field, applying a post-emergence effective dose of dicamba herbicide sufficient to kill the toxic weed species to the field, and harvesting seed from the field. A herbicidally effective dose of dicamba for use in the field should consist of a range from about 0.005 pounds per acre to about 8 pounds of dicamba per acre over a growing season. Multiple applications of dicamba may be used over a growing season, for example, two applications (such as a pre-planting application and a post-emergence application or a pre-emergence application and a post-emergence application) or three applications (such as a pre-planting application, a pre-emergence application, and a post-emergence application).
Methods for producing an herbicide tolerant soybean plant comprising the DNA sequences specific and unique to the transgenic event MON 87708 of the invention are provided. Transgenic plants used in these methods may be homozygous or heterozygous for the transgene. Progeny plants produced by these methods may be varietal or hybrid plants; may be grown from seeds produced by a soybean event MON 87708 plant and/or from seeds produced by a plant fertilized with pollen from a soybean event MON 87708 plant; and may be homozygous or heterozygous for the transgene. Progeny plants may be subsequently self-pollinated to generate a true breeding line of plants, i.e. , plants homozygous for the transgene, or alternatively may be outcrossed, e.g., bred with another unrelated plant, to produce a varietal or a hybrid seed or plant.
A soybean plant that tolerates application of dicamba herbicide may be produced by sexually crossing an event MON 87708 plant comprising a nucleotide molecule comprising the sequence of SEQ ID NO: 1 and SEQ ID NO: 2 with another soybean plant and thereby producing seed, which is then grown into progeny plants. These progeny plants may then be treated with dicamba herbicide to select for progeny plants that are tolerant to dicamba herbicide. Alternatively, these progeny plants may be analyzed using diagnostic methods to select for progeny plants that contain the event MON 87708 DNA. The other plant used in the crossing may or may not be tolerant to dicamba herbicide and may or may not be transgenic. The progeny plant and/or seed produced may be varietal or hybrid seed. In practicing this method, the step of sexually crossing one plant with another plant, i.e., cross-pollinating, may be accomplished or facilitated by human intervention, for example: by human hands collecting the pollen of one plant and contacting this pollen with the style or stigma of a second plant; by human hands and/or actions removing, destroying, or covering the stamen or anthers of a plant (e.g., by detasseling or by application of a chemical gametocide) so that natural self-pollination is prevented and cross-pollination would have to take place in order for fertilization to occur; by human placement of pollinating insects in a position for "directed pollination" (e.g., by placing beehives in orchards or fields or by caging plants with pollinating insects); by human opening or removing of parts of the flower to allow for placement or contact of foreign pollen on the style or stigma (e.g. , in soy which naturally has flowers that hinder or prevent cross-pollination, making them naturally obligate self-pollinators without human intervention); by selective placement of plants (e.g., intentionally planting plants in pollinating proximity); and/or by application of chemicals to precipitate flowering or to foster receptivity (of the stigma for pollen).
A soybean plant that tolerates application of dicamba herbicide may be produced by selfing an event MON 87708 plant comprising a nucleotide molecule comprising the sequence of SEQ ID NO: 1 and SEQ ID NO: 2 and thereby producing seed, which is then grown into progeny plants. These progeny plants may then be treated with dicamba herbicide to select for progeny plants that are tolerant to dicamba herbicide. Alternatively, these progeny plants may be analyzed using diagnostic methods to select for progeny plants that contain the event MON 87708 DNA. In practicing this method, the step of sexually crossing one plant with itself, i.e. , self-pollinating or selfing, may be accomplished or facilitated by human intervention, for example: by human hands collecting the pollen of the plant and contacting this pollen with the style or stigma of the same plant and then optionally preventing further fertilization of the plant; by human hands and/or actions removing, destroying, or covering the stamen or anthers of other nearby plants (e.g. , by detasseling or by application of a chemical gametocide) so that natural cross-pollination is prevented and self-pollination would have to take place in order for fertilization to occur; by human placement of pollinating insects in a position for "directed pollination" (e.g., by caging a plant alone with pollinating insects); by human manipulation of the flower or its parts to allow for self-pollination; by selective placement of plants (e.g., intentionally planting plants beyond pollinating proximity); and/or by application of chemicals to precipitate flowering or to foster receptivity (of the stigma for pollen).
Progeny soybean plants and seeds encompassed by these methods and produced by using these methods will be distinct from other soybean plants, for example because the progeny soybean plants and seeds: are recombinant and as such created by human intervention; are dicamba herbicide tolerant; contain at least one allele that consists of the transgene DNA of the invention; and/or contain a detectable amount of a polynucleotide sequence selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO: 2. A seed may be selected from an individual progeny plant, and so long as the seed comprises SEQ ID NO: 1 and SEQ ID NO: 2, it will be within the scope of the invention.
In practicing the invention, two different transgenic plants can be crossed to produce hybrid offspring that contain two independently segregating heterologous genes. Selling of appropriate progeny can produce plants that are homozygous for both genes. Back-crossing to a parental plant and out-crossing with a non-transgenic plant are also contemplated, as is vegetative propagation. Descriptions of other methods that are commonly used for different traits and crops can be found in one of several references, e.g., Fehr, in Breeding Methods for Cultivar Development, Wilcox J. ed., American Society of Agronomy, Madison WI (1987).
The plants and seeds used in the methods disclosed herein may also contain one or more additional transgenes. Such transgene may be any nucleotide sequence encoding a protein or RNA molecule conferring a desirable trait including but not limited to increased insect resistance, increased water use efficiency, increased yield performance, increased drought resistance, increased seed quality, improved nutritional quality, and/or increased herbicide tolerance, in which the desirable trait is measured with respect to a soybean plant lacking such additional transgene.
The methods of the invention are therefore useful for, among other things, controlling weeds in a field while growing plants for the purpose of producing seed and/or plant parts of soybean event MON 87708 for agricultural or research purposes, selecting for progeny of soybean event MON 87708 for plant breeding or research purposes, and producing progeny plants and seeds of soybean event MON 87708.
The plants, progeny, seeds, plant cells, plant parts (such as pollen, ovule, pod, flower, root or stem tissue, and leaves), and commodity products of the invention may be evaluated for DNA composition, gene expression, and/or protein expression. Such evaluation may be done by using any standard method such as PCR, northern blotting, southern analysis, western blotting, immuno-precipitation, and ELISA or by using the methods of detection and/or the detection kits provided herein. Methods of detecting the presence of DNA derived from a soybean cell, tissue, seed, or plant of soybean event MON 87708 in a sample are provided. One method consists of (i) extracting a DNA sample from at least one soybean cell, tissue, seed, or plant, (ii) contacting the DNA sample with a primer pair that is capable of producing an amplicon from event MON 87708 DNA under conditions appropriate for DNA amplification, (iii) performing a DNA amplification reaction, and then (iv) detecting the amplicon molecule and/or confirming that the nucleotide sequence of the amplicon comprises a nucleotide sequence specific for event MON 87708, such as one selected from the group consisting of SEQ ID NO: 1-8. The amplicon should be one that is specific for event MON 87708, such as an amplicon that comprises SEQ ID NO: 1 or SEQ ID NO: 2. The detection of a nucleotide sequence specific for event MON 87708 in the amplicon is determinative and/or diagnostic for the presence of the soybean event MON 87708 specific DNA in the sample. An example of a primer pair that is capable of producing an amplicon from event MON 87708 DNA under conditions appropriate for DNA amplification is provided as SEQ ID NO: 10-11. Other primer pairs may be readily designed by one of skill in the art and would comprise at least one fragment of SEQ ID NO: 6. Another method of detecting the presence of DNA derived from a soybean cell, tissue, seed, or plant of soybean event MON 87708 in a sample consists of (i) extracting a DNA sample from at least one soybean cell, tissue, seed, or plant,, (ii) contacting the DNA sample with a DNA probe specific for event MON 87708 DNA, (iii) allowing the probe and the DNA sample to hybridize under stringent hybridization conditions, and then (iv) detecting hybridization between the probe and the target DNA sample. An example of the sequence a DNA probe that is specific for event MON 87708 DNA is provided as SEQ ID NO: 11. Other probes may be readily designed by one of skill in the art and would comprise at least one fragment of SEQ ID NO: 6. Detection of probe hybridization to the DNA sample is diagnostic for the presence of soybean event MON 87708 specific DNA in the sample. Absence of hybridization is alternatively diagnostic of the absence of soybean event MON 87708 specific DNA in the sample.
DNA detection kits are provided that are useful for the identification of soybean event MON 87708 DNA in a sample and can also be applied to methods for breeding soybean plants containing the appropriate event DNA. Such kits contain DNA primers and/or probes comprising fragments of SEQ ID NO: 1-8. One example of such a kit comprises at least one DNA molecule of sufficient length of contiguous nucleotides of SEQ ID NO: 6 to function as a DNA probe useful for detecting the presence and/or absence of DNA derived from transgenic soybean event MON 87708 in a sample. The DNA derived from transgenic soybean event MON 87708 would comprise SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8. A DNA molecule sufficient for use as a DNA probe is provided that is useful for determining, detecting, or diagnosing the presence and/or absence of soybean event MON 87708 DNA in a sample is provided as SEQ ID NO: 11. Other probes may be readily designed by one of skill in the art and should comprise at least 15 contiguous nucleotides of SEQ ID NO: 6 and be sufficiently unique to soybean event MON 87708 DNA in order to identify DNA derived from the event. Another type of kit comprises a primer pair useful for producing an amplicon useful for detecting the presence and/or absence of DNA derived from transgenic soybean event MON 87708 in a sample. Such a kit would employ a method comprising contacting a target DNA sample with a primer pair as described herein, then performing a nucleic acid amplification reaction sufficient to produce an amplicon comprising SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 7, and/or SEQ ID NO: 8, and then detecting the presence and/or absence of the amplicon. Such a method may also include sequencing the amplicon or a fragment thereof, which would be determinative of, i.e. diagnostic for, the presence of the soybean event MON 87708 specific DNA in the target DNA sample. Other primer pairs may be readily designed by one of skill in the art and should comprise at least 15 contiguous nucleotides of SEQ ID NO: 6 and be sufficiently unique to soybean event MON 87708 DNA in order to identify DNA derived from the event.
Nucleic-acid amplification can be accomplished by any of the various nucleic-acid amplification methods known in the art, including thermal amplification methods. Many techniques are known in the art for detecting, quantifying, and/or sequencing the amplicon produced by these methods. One exemplary technique useful in practicing this invention is TAQMAN® (PE Applied Biosystems, Foster City, CA).
The kits and detection methods of the invention are useful for, among other things, identifying soybean event MON 87708, selecting plant varieties or hybrids comprising soybean event MON 87708, detecting the presence of DNA derived from the transgenic soybean event MON 87708 in a sample, and monitoring samples for the presence and/or absence of soybean event MON 87708 or plant parts derived from soybean event MON 87708.
The sequence of the heterologous DNA insert, junction sequences, or flanking sequences from soybean event MON 87708 (with representative seed samples deposited as ATCC PTA-9670) can be verified (and corrected if necessary) by amplifying such sequences from the event using primers derived from the sequences provided herein followed by standard DNA sequencing of the amplicon or of the cloned DNA.
As used herein, the term "comprising" means "including but not limited to".
The following examples are included to demonstrate examples of certain preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples that follow represent approaches the inventors have found function well in the practice of the invention, and thus can be considered to constitute examples of preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments that are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.
EXAMPLES
Example 1: Transformation of Soybean A3525 and MON 87708 event selection
The soybean plant MON 87708 was produced by Agrobacterium-mediated transformation of soybean. Soybean cells were transformed and regenerated into intact soybean plants and individual plants were selected from the population of plants that showed integrity of the plant expression cassette and resistance to dicamba. From this population, soybean plant event MON 87708 was selected and characterized.
The transgenic dicamba tolerant soybean plant MON 87708 was developed through Agrobacterium-mediated transformation of soybean meristem tissue utilizing transformation vector PV- GMHT4355. The method was described in U.S. Patent No. 6,384,301 (herein incorporated by reference), which allows for the generation of transformed plants without utilization of callus. Briefly, meristem tissues were excised from the embryos of germinated A3525 soybean seed (Asgrow, St Louis, MO). After co-culturing with Agrobacterium carrying the vector, the meristems were placed on selection medium containing glyphosate (Monsanto, St Louis, MO), carbenicillin disodium salt, cefotaxime sodium salt, and ticarcillin disodium salt/potassium clavulanate mixture to inhibit the growth of untransformed plant cells and excess Agrobacterium. The meristems were then placed in media conducive to shoot and root development. Rooted plants with normal phenotypic characteristics were selected and transferred to soil for growth and further assessment.
The R0 plants generated through the above transformation were transferred to soil for growth and then selfed to produce Rl seed. During subsequent selfrng of the R0 plants to produce the Rl generation, the unlinked insertions of T-DNA I (dmo expression cassette) and T-DNA II (cp4 epsps expression cassette) were segregated. A non-lethal dose of glyphosate was applied to Rl plants. The plants with minor injuries were selected for further analyses, whereas plants showing no injury, i.e., containing T-DNA II (cp4 epsps expression cassette) were eliminated from subsequent development. Subsequently, R0 plants containing only a single T-DNA I insert (i.e., dmo gene cassette) were identified. The T-DNA I expression cassette comprised the Peanut Chlorotic Streak Virus (PC1SV) promoter with a duplicated enhancer region (P-PClSV.FLt-enh); operably linked to a DNA leader derived from RNA transcript of Tobacco Etch Virus (L-TEV); operably linked to a DNA molecule encoding an N-terminal chloroplast transit peptide from ribulose 1,5-bisphosphate carboxylase small subunit (SSU) from Pisum sativum (TS-RbcS-3C); operably linked to part of the mature protein from ribulose 1,5-bisphosphate carboxylase small subunit (SSU) from Pisum sativum (CR-RbcS-3C); operably linked to a DNA molecule encoding a dicamba mono-oxygenase (DMO) from Stenotrophomonas maltophilia (Pseudomonas maltophilia was the original name of the source of the DMO gene. This source organism was subsequently reclassified first as Xanthomonas maltophilia and then as Stenotrophomonas maltophilia); operably linked to a 3 ' UTR DNA molecule derived from the ribulose 1,5-bisphosphate carboxylase small subunit gene of Pisum sativum (T-Ps.RbcS2- E9). Plants were selected by a combination of analytical techniques, including TaqMan, PCR analysis, and herbicide spray. The MON 87708 event was selected from among approximately 2,400 individual transgenic events based on its superior phenotypic characteristics, a comprehensive molecular profile analysis, and its desirable haplotype association. Event MON 87708 was then crossed with event MON 89788 (glyphosate tolerant). The progeny of this cross were treated with dicamba (Clarity®, BASF, Research Triangle Park, NC), glyphosate (Roundup WeatherMAX®, Monsanto Co., St Louis, MO), or a combination of dicamba and glyphosate. The treatments were done at pre-plant, post-plant at the vegetative 3 growth stage (V3), and post-plant at the reproduction 1 stage (Rl). Treated plants were scored for percent growth inhibition at 14 days after treatment (DAT) for the pre-plant herbicide treatment, 3 DAT for post-emergence treatment at the VE stage, and 3 DAT post-emergence treatment at the Rl stage. The herbicide(s) were applied at various rates per acre as shown in Table 1. Percent inhibition measurements represent an average of the repetitions.
Table 1: Dicamba and/or Roundup WeatherMAX® tolerance testing with MON89788 x MON 87708
Figure imgf000027_0001
The dicamba tolerance transgene was mapped in soybean event MON 87708 to linkage group 9 at approximately map position 143.5. The associated haplotype window 19743 and 19767 has no effect on yield, maturity, height or lodging. Haplotype association information is provided in Table 2 where GM_A92205 indicates event MON 87708.
Table 2: Haplotype association LG9, Pos 143.5
Figure imgf000027_0002
GM_A92205 19743 1573373 0.00 -0.20 -0.01 -0.03 TG*GG 9
GM_A92205 19743 1573374 0.00 -0.08 -0.07 0.05 TG*GA 9
GM_A92205 19743 1573375 0.00 -0.15 0.05 0.04 CCCTA 9
GM_A92205 19743 1573376 0.00 -0.45 -0.14 0.00 TC*GG 9
GM_A92205 19767 1573486 0.00 0.00 -0.03 0.00 TACGGTC 9
GM_A92205 19767 1573493 0.00 0.00 0.22 0.00 AACAATT 9
GM_A92205 19767 1573494 0.00 0.00 0.03 0.00 TACAATC 9
GM_A92205 19767 1573495 0.00 0.00 0.07 0.00 TGAAACC 9
GM_A92205 19767 1573497 0.00 0.00 0.41 0.00 TACGGTT 9
GM_A92205 19767 1573499 0.00 0.00 -0.01 0.00 TGAAACT 9
GM_A92205 19767 1573500 0.00 0.00 0.06 0.00 TGAGACC 9
GM_A92205 19767 1573502 0.00 0.00 -0.07 0.00 AACAATC 9
GM_A92205 19767 1573503 0.00 0.00 0.08 0.00 AACGATC 9
GM_A92205 19767 1573504 0.00 0.00 0.07 0.00 TACAGTC 9
GM_A92205 19767 1573506 0.00 0.00 -0.03 0.00 AACGATT 9
GM_A92205 19767 1573507 0.00 0.00 0.20 0.00 TGAAATT 9
Example 2: Characterization of MON 87708 DNA sequences
The DNA inserted into the genome of soybean plant MON 87708 and the flanking sequence was characterized by detailed molecular analyses. These analyses included: the insert sequence, the insert number (number of integration sites within the soybean genome), the copy number (number of copies of transgene DNA within one locus), the integrity of the inserted gene cassette, the flanking sequences, and the association of the insertion with haplotype regions of the soybean genome.
Molecular DNA probes were used that included the intact coding region and its respective regulatory elements, the promoters, introns, and polyadenylation sequences of the plant expression cassettes. The analysis showed that MON 87708 contains a single transgene DNA insertion with one copy of the expression cassette. Inverse PCR and DNA sequence analyses were performed to determine the 5' and 3 ' insert-to-plant genome junctions, confirm the organization of the elements within the insert (Figure 1), and determine the complete DNA sequence of the insert in soybean plant MON 87708 (provided herein as SEQ ID NO: 5). A soybean plant that comprises in its genome the linked transgene genetic elements shown in Figure 1 and is resistant to dicamba is an aspect of the invention.
Sequences flanking the transgene DNA insertion in MON 87708 were determined using inverse PCR as described in Ochman et ah, 1990 (PCR Protocols: A guide to Methods and Applications, Academic Press, Inc.) and/or genome walker techniques. Plant genomic DNA was isolated from both A3525 and the transgenic soybean lines from tissue grown under standard greenhouse conditions. Approximately 1 gram of young leaf tissue was combined with liquid nitrogen and ground to a fine powder using a mortar and pestle. DNA was extracted using a Nucleon™ PhytoPure™ Genomic DNA extraction kit (RPN8511, Amersham, Piscataway, NJ) according to the manufacturer's protocol. After the final precipitation step, DNA was resuspended in 0.5 ml of TE (lOmM Tris-HCl pH 8.0, ImM EDTA). This method can be modified by one skilled in the art to extract DNA from any tissue of soybean, including, but not limited to seed. An aliquot of DNA was digested with restriction endonucleases selected based upon restriction analysis of the transgene DNA. After self-ligation of restriction fragments, PCR was performed using primers designed from the transgene DNA sequence that would amplify sequences extending away from the 5' and 3 ' ends of the transgene DNA. PCR products were separated by agarose gel electrophoresis and purified using a QIAGEN gel purification kit (Qiagen, Valencia, CA). The subsequent DNA products were sequenced directly using standard DNA sequencing protocols. The 5' flanking sequence which extends into the right border sequence of the expression cassette transgene DNA is presented as SEQ ID NO: 3 ([C], see Figure 1). The 3 ' flanking sequence which extends into the left border sequence of the expression cassette transgene DNA is presented as SEQ ID NO: 4 ([D], see Figure 1). The portion of the expression cassette DNA that was fully integrated into the A3525 genomic DNA is presented as SEQ ID NO: 5 ([E], see Figure 1).
Isolated DNA molecule sequences were compared to the transgene DNA sequence to identify the flanking sequence and the co-isolated transgene DNA fragment. Confirmation of the presence of the expression cassette was achieved by PCR with primers designed based upon the deduced flanking sequence data and the known transgene DNA sequence. The wild type sequence corresponding to the same region in which the transgene DNA was integrated in the transformed line was isolated using primers designed from the flanking sequences in MON 87708. The PCR reactions were performed using the Elongase® amplification system (Invitrogen, Carlsbad, CA). The flanking DNA sequences in MON 87708 and the A3525 wild type sequence were analyzed against multiple nucleotide and protein databases. This information was used to examine the relationship of the transgene to the plant genome and to look for the insertion site integrity. The flanking sequence and wild type sequences were used to design primers for TAQMAN® endpoint assays used to identify the events. Zygosity assays were developed using this information.
Example 3: Event specific endpoint TAQMAN® assays.
This example describes an event specific endpoint TAQMAN® thermal amplification method developed to identify event MON 87708 in a sample. Examples of conditions useful with the event MON 87708 Specific Endpoint TAQMAN® method are as follows: Step 1: 18 megohm water adjusted for final volume of 10 μΐ. Step 2: 5.0 μΐ of 2X Universal Master Mix (dNTPs, enzyme, buffer) to a IX final concentration. Step 3: 0.5 μΐ Event Primer-1 (SQ13570) and Event Primer-2 (SQ13571) Mix (resuspended in 18 megohm water to a concentration of 20 uM for each primer) to 1.0 μΜ final concentration (for example in a microcentrifuge tube, the following should be added to achieve 500 μΐ at a final concentration of 20uM: 100 μΐ of Primer SQ13570 (SEQ ID NO: 9) at a concentration of 100 μΜ; 100 μΐ of Primer SQ13571 (SEQ ID NO: 10) at a concentration of 100 μΜ; 300 μΐ of 18 megohm water). Step 4: 0.2 μΐ Event 6-FAM™ MGB Probe PB4655 (resuspended in 18 megohm water to a concentration of 10 μΜ (SEQ ID NO: 11) to 0.2 μΜ final concentration. Step 5: 0.5 μΐ Internal Control Primer-1 and Internal Control Primer-2 Mix (resuspended in 18 megohm water to a concentration of 20 μΜ for each primer) to 1.0 μΜ final concentration. Step 6: 0.2 μΐ Internal Control VIC™ Probe to 0.2 μΜ final concentration (resuspended in 18 megohm water to a concentration of 10 μΜ) Step 7: 3.0 μΐ Extracted DNA (template) for each sample with one each of the following comprising 1. Leaf Samples to be analyzed; 2. Negative control (non-transgenic DNA); 3. Negative water control (no template); 4. Positive control MON 87708 DNA. Step 8: Thermocycler Conditions as follows: One Cycle at 50°C for 2 minutes; One Cycle at 95°C forlO minutes; Ten Cycles of 95°C for 15 seconds then 64°C for 1 minute with -l°C/cycle; Thirty Cycles of 95°C for 15 seconds then 54°C 1 minute; final cycle of 10°C. The DNA primers used in the endpoint assay are primers SQ13570 (SEQ ID NO: 9), SQ13571 (SEQ ID NO: 10), and 6-FAM™ labeled probe PB4655 (SEQ ID NO: 11). 6-FAM™ is a fluorescent dye product of Applied Biosystems (Foster City, CA) attached to the DNA probe. For TAQMAN® MGB™ probes, the 5'exonuclease activity of Taq DNA polymerase cleaves the probe from the 5'-end, between the fluorophore and quencher. When hybridized to the target DNA strand, quencher and fluorophore are separated enough to produce a fluorescent signal, thus releasing fluorescence. SQ13570 (SEQ ID NO: 9) and SQ13571 (SEQ ID NO: 10) when used with these reaction methods with PB4655 (SEQ ID NO: 11) produce a DNA amplicon that is diagnostic for event MON 87708 DNA. The controls for this analysis should include a positive control from soybean containing event MON 87708 DNA, a negative control from non-transgenic soybean, and a negative control that contains no template DNA. Additionally, a control for the PCR reaction includes Internal Control Primers and an Internal Control Probe, specific to a single copy gene in the Glycine genome. One of skill in the art will know how to design primers specific to a single copy gene in the Glycine genome. These assays are optimized for use with either an Applied Biosystems GeneAmp® PCR System 9700 (run at maximum speed) or MJ Research DNA Engine PTC- 225 thermal cycler. Other methods and apparatus known to those skilled in the art that produce amplicons that identify the event MON 87708 DNA is within the skill of the art.
R0 plants demonstrating the presence of the expression cassette were allowed to develop into fully mature plants. Probes designed based on the sequences of the dicamba tolerance transgene cassette were used to probe Southern blots to determine linkage. The R0 plants were also evaluated for copy number of the expression cassette using a combination of Southern analysis and endpoint TAQMAN®.
A zygosity assay is useful for determining if a plant comprising an event is homozygous for the event DNA; that is comprising the exogenous DNA in the same location on each chromosome of a chromosomal pair; or heterozygous for an event DNA, that is comprising the exogenous DNA on only one chromosome of a chromosomal pair; or is null for the event DNA, that is wildtype. The endpoint TAQMAN® thermal amplification method was also used to develop zygosity assays for event MON 87708. This example describes an event specific endpoint TAQMAN® thermal amplification method developed to determine the zygosity of event MON 87708 in a sample. For this assay, a three primer assay was employed wherein primer SQ20632 (SEQ ID NO: 12) hybridizes and extends specifically from the 3' junction of the inserted exogenous DNA and genomic DNA, primer SQ20636 (SEQ ID NO: 13) hybridizes and extends specifically from the DNA flanking the 3' side of the inserted exogenous DNA, and primer SQ20637 (SEQ ID NO: 14) hybridizes and extends specifically from genomic DNA into which was integrated the inserted exogenous DNA. The three primers are diagnostic for the event. In this example, primer SQ20636 (SEQ ID NO: 13) and primer SQ20632 (SEQ ID NO: 12) and the 6-FAM™-labeled oligonucleotide probe PB10130 (SEQ ID NO: 15) are diagnostic when there is a copy of the inserted exogenous DNA. In this example, SQ20636 (SEQ ID NO: 13) and primer SQ20637 (SEQ ID NO: 14) and the VIC™-labeled oligonucleotide probe PB10131 (SEQ ID NO: 16) are diagnostic when there is no copy of the inserted exogenous DNA present in the genomic DNA, i.e. wildtype. When the three primers and two probes are mixed together in a PCR reaction with DNA extracted from a plant homozygous for event MON 87708, there is a fluorescent signal only from the 6- FAM™-labeled oligonucleotide probe PB10130 (SEQ ID NO: 15) which is indicative of and diagnostic a plant homozygous for event MON 87708. When the three primers and two probes are mixed together in a PCR reaction with DNA extracted from a plant heterozygous for event MON 87708, there is a fluorescent signal from both the 6-F AM™-labeled oligonucleotide probe PB 10130 (SEQ ID NO: 15) and the VIC™- labeled oligonucleotide probe PB10131 (SEQ ID NO: 16) which is indicative of and diagnostic a plant heterozygous for event MON 87708. When the three primers and two probes are mixed together in a PCR reaction with DNA extracted from a plant which is null for event MON 87708 {i.e. wildtype), there is a fluorescent signal from only the VIC™-labeled oligonucleotide probe PB10131 (SEQ ID NO: 16) which is indicative of and diagnostic a plant null for event MON 87708, i.e. wildtype. Examples of conditions useful with this method are as follows. Step 1 : 18 megohm water adjusted for final volume of 10 μΐ. Step 2: 5.0 μΐ of 2X Universal Master Mix (Applied Biosystems cat # 4304437; dNTPs, enzyme, buffer) to a IX final concentration. Step 3: 0.5 μΐ of Zygosity Primers SQ20632, SQ20636, SQ20637 (resuspended in 18 megohm water to a concentration of 20 μΜ for each primer) to a final concentration of 1.0 μΜ. Step 4: 0.2 μΐ 6-FAM™ Probe PB 10130 (SEQ ID NO: 15) (resuspended in 18 megohm water to a concentration of 10 μΜ) to 0.2 μΜ final concentration. Step 5: 0.2 μΐ VIC™ Probe PB10131 (SEQ ID NO: 16) (resuspended in 18 megohm water to a concentration of 10 μΜ) to 0.2 μΜ final concentration. Step 6: 3.0 μΐ Extracted DNA (template) for each sample with one each of the following comprising 1. Leaf Samples to be analyzed (4-80 ng of genomic DNA diluted in water); 2. Negative control (non-transgenic soybean DNA; 4ng diluted in water); 3. Negative water control (no template; solution in which DNA was resuspended); 4. Positive control MON 87708 genomic DNA from known heterozygous event (4 ng diluted in water); 5. 4. Positive control MON 87708 genomic DNA from known homozygous event (4 ng diluted in water). Step 7: Gently mix. Step 8: Thermocycler Conditions when using Applied Biosystems GeneAmp® PCR System 9700 (run at maximum speed) or MJ Research DNA Engine PTC-225 thermal cycler are as follows: One Cycle at 50°C for 2 minutes; one cycle at 95°C for 10 minutes; Ten Cycles of (95°C for 15 seconds then 64°C for 1 minute (-l°C/cycle); Thirty Cycles of (95°C for 15 seconds then 54°C for 1 minute); Optional additional 10 to 20 cycles (95°C for 15 seconds then 64°C for 1 minute (-l°C/cycle) may provide more distinct population separation during EndPoint TaqMan® analysis; One cycle at 10°C hold. Example 4: Identification of event MON 87708 in any MON 87708 breeding activity
The following example describes how one may identify the MON 87708 event within progeny of any breeding activity using soybean event MON 87708.
DNA event primer pairs are used to produce an amplicon diagnostic for soybean event MON 87708. An amplicon diagnostic for MON 87708 comprises at least one junction sequence, provided as SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 7 or SEQ ID NO: 8. Event primer pairs that will produce a diagnostic amplicon for MON 87708 include primer pairs based upon the flanking sequences and the inserted expression cassette. To acquire a diagnostic amplicon in which SEQ ID NO: 1 is found, one would design a forward primer molecule based upon SEQ ID NO: 3 from bases 1 through 1126 and a reverse primer molecule based upon the inserted expression cassette DNA sequence (SEQ ID NO: 5 from positions 1 through 3003) in which the primer molecules are of sufficient length of contiguous nucleotides to specifically hybridize to SEQ ID NO: 3 and SEQ ID NO: 5. To acquire a diagnostic amplicon in which SEQ ID NO: 2 is found, one would design a forward primer molecule based upon the inserted expression cassette DNA sequence (SEQ ID NO: 5 from positions 1 through 3003) and a reverse primer molecule based upon the 3 ' flanking sequence (SEQ ID NO: 4 from bases 131 through 1947), in which the primer molecules are of sufficient length of contiguous nucleotides to specifically hybridize to SEQ ID NO: 4 and SEQ ID NO: 5. For practical purposes, one should design primers which produce amplicons of a limited size range, for example, between 100 to 1000 bases. Smaller (shorter polynucleotide length) sized amplicons in general are more reliably produced in PCR reactions, allow for shorter cycle times, and can be easily separated and visualized on agarose gels or adapted for use in endpoint TAQMAN®-like assays. Smaller amplicons can be produced and detected by methods known in the art of DNA amplicon detection. In addition, amplicons produced using the primer pairs can be cloned into vectors, propagated, isolated, and sequenced or can be sequenced directly with methods well established in the art. Any primer pair derived from the combination of SEQ ID NO: 3 and SEQ ID NO: 5 or the combination of SEQ ID NO: 4 and SEQ ID NO: 5 that are useful in a DNA amplification method to produce an amplicon diagnostic for MON 87708 or progeny thereof is an aspect of the invention. Any single isolated DNA polynucleotide primer molecule comprising at least 11 contiguous nucleotides of SEQ ID NO: 3, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for MON 87708 or progeny thereof is an aspect of the invention. Any single isolated DNA polynucleotide primer molecule comprising at least 11 contiguous nucleotides of SEQ ID NO: 4, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for MON 87708 or progeny thereof is an aspect of the invention. Any single isolated DNA polynucleotide primer molecule comprising at least 11 contiguous nucleotides of SEQ ID NO: 5, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for MON 87708 or progeny thereof is an aspect of the invention.
An example of the amplification conditions for this analysis is illustrated in Example 3. However, any modification of these methods or the use of DNA primers homologous or complementary to SEQ ID NO: 3 or SEQ ID NO: 4 or DNA sequences of the genetic elements contained in the transgene insert (SEQ ID NO: 5) of MON 87708 that produce an amplicon diagnostic for MON 87708 is within the art. A diagnostic amplicon comprises a DNA molecule homologous or complementary to at least one transgene/genomic junction DNA (SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 7 or SEQ ID NO: 8), or a substantial portion thereof.
An analysis for event MON 87708 plant tissue sample should include a positive tissue control from event MON 87708, a negative control from a soybean plant that is not event MON 87708 (for example, but not limited to A3525), and a negative control that contains no soybean genomic DNA. A primer pair that will amplify an endogenous soybean DNA molecule will serve as an internal control for the DNA amplification conditions. Additional primer sequences can be selected from SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5 by those skilled in the art of DNA amplification methods, and conditions selected for the production of an amplicon by the methods shown in Example 3 may differ, but result in an amplicon diagnostic for event MON 87708 DNA. The use of these DNA primer sequences with modifications to the methods of Example 3 are within the scope of the invention. The amplicon produced by at least one DNA primer sequence derived from SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5 that is diagnostic for MON 87708 is an aspect of the invention.
DNA detection kits contain at least one DNA primer of sufficient length of contiguous nucleotides derived from SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5, that when used in a DNA amplification method produces a diagnostic amplicon for MON 87708 or its progeny is an aspect of the invention. A MON 87708 soybean plant, plant part, plant cell, seed, or commodity product that will produce an amplicon diagnostic for MON 87708 when tested in a DNA amplification method is an aspect of the invention. The assay for the MON 87708 amplicon can be performed by using an Applied Biosystems GeneAmp® PCR System 9700 (run at maximum speed) or MJ Research DNA Engine PTC-225 thermal cycler or any other amplification system that can be used to produce an amplicon diagnostic of MON 87708 as shown in Example 3.
A deposit of a representative sample of soybean event MON 87708 seed disclosed above and recited in the claims has been made under the Budapest Treaty with the American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA. 20110. The ATCC accession number for this deposit is PTA-9670. The deposit will be maintained in the depository for a period of 30 years, or 5 years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced as necessary during that period.
Having illustrated and described the principles of the invention, it should be apparent to persons skilled in the art that the invention can be modified in arrangement and detail without departing from such principles. We claim all modifications that are within the spirit and scope of the appended claims.

Claims

We Claim:
1. A recombinant DNA molecule comprising a nucleotide molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8, and complements thereof.
2. The recombinant DNA molecule of claim 1 , wherein said recombinant DNA molecule was formed by the insertion of a heterologous nucleic acid molecule into the genomic DNA of a soybean plant, plant cell, or seed.
3. The recombinant DNA molecule of claim 1, wherein said DNA molecule is derived from transgenic soybean event MON 87708, a representative sample of which has been deposited as ATCC PTA-9670.
4. The recombinant DNA molecule of claim 1, wherein said DNA molecule is in a soybean plant, plant cell, seed, plant part, or commodity product derived from soybean event MON 87708, a representative sample of which has been deposited as ATCC PTA-9670.
5. The recombinant DNA molecule of claim 1, wherein said DNA molecule is an amplicon diagnostic for the presence of DNA derived from event MON 87708.
6. A DNA molecule comprising a nucleotide molecule having a nucleotide sequence of sufficient length of contiguous nucleotide sequence of SEQ ID NO: 6, and complements thereof, to function as a DNA probe that hybridizes under stringent hybridization conditions with a DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8 and does not hybridize under the stringent hybridization conditions with a DNA molecule not comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8.
7. A pair of DNA molecules consisting of a first DNA molecule and a second DNA molecule different from the first DNA molecule, wherein said first and second DNA molecules each comprise a nucleotide molecule having a nucleotide sequence of sufficient length of contiguous nucleotides of SEQ ID NO: 6, or a complement thereof, to function as DNA primers when used together in an amplification reaction with DNA derived from event MON 87708 to produce an amplicon diagnostic for soybean event MON 87708 DNA in a sample.
8. A method of detecting the presence of a DNA molecule derived from soybean event MON 87708 in a sample, said method comprising:
a. contacting a sample with the DNA probe of claim 6; b. subjecting said sample and said DNA probe to stringent hybridization conditions; and c. detecting hybridization of said DNA probe to a DNA molecule in said sample, wherein the
hybridization of said DNA probe to said DNA molecule indicates the presence of a DNA molecule derived from soybean event MON 87708 in said sample.
9. A method of detecting the presence of a DNA molecule derived from soybean event MON 87708 in a sample, said method comprising:
a. contacting a sample with the pair of DNA molecules of claim 7;
b. performing an amplification reaction sufficient to produce a DNA amplicon comprising a
sequence selected from the group consisting of SEQ ID NO: 1-8, and complements thereof; and c. detecting the presence of said DNA amplicon in said reaction, wherein the presence of said DNA amplicon in said reaction indicates the presence of a DNA molecule derived from soybean event MON 87708 in said sample.
10. A DNA detection kit comprising at least one DNA molecule comprising a nucleotide sequence of sufficient length of contiguous nucleotide sequence of SEQ ID NO: 6, and complements thereof, to function as a DNA primer or probe specific for detecting the presence of DNA derived from soybean event MON 87708, wherein detection of said DNA is diagnostic for the presence of said event MON 87708 DNA in a sample.
11. A recombinant soybean plant, seed, cell, or plant part thereof comprising a nucleotide molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8, and complements thereof.
12. The recombinant soybean plant, seed, cell, or plant part thereof of claim 11, wherein said plant, seed, cell, or plant part thereof is tolerant to dicamba herbicide treatment.
13. The recombinant soybean plant, seed, cell, or plant part thereof of claim 11, the genome of which produces an amplicon comprising a DNA molecule selected from the group consisting of SEQ ID NO: 1- 8, and complements thereof, when tested in a DNA amplification method.
14. A soybean plant or seed, wherein said soybean plant or seed is derived from soybean event
MON 87708, a representative sample having been deposited under ATCC accession No. PTA-9670.
15. The soybean plant or seed of claim 14, wherein said soybean plant or seed is a hybrid having at least one parent derived from soybean event MON 87708.
16. A nonliving plant material comprising a recombinant DNA molecule selected from the group
consisting of SEQ ID NO: 1-8, and complements thereof.
17. A microorganism comprising a nucleotide molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8, and complements thereof.
18. The microorganism of claim 17, wherein said microorganism is a plant cell.
19. A commodity product comprising a nucleotide molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8, and complements thereof.
20. The commodity product of claim 19, wherein said commodity product is selected from the group consisting of whole or processed seeds, animal feed, oil, meal, flour, flakes, bran, milk, cheese, paper, cream, wine, biomass, and fuel products.
21. A method for controlling weeds in a field comprising planting soybean event MON 87708 plants in a field and applying an effective dose of dicamba herbicide to control weeds in said field without injuring said soybean event MON 87708 plants.
22. The method of claim 21, wherein said effective dose of dicamba herbicide is from about 0.005 pounds to about 8 pounds per acre.
23. A method for controlling weeds in a field comprising applying an effective dose of dicamba herbicide to control weeds in a field and then planting soybean event MON 87708 in said field.
24. The method of claim 23, wherein said effective dose of dicamba herbicide is from about 0.005 pounds to about 8 pounds per acre and said planting soybean event MON 87708 is within 14 days of said applying an effective dose of dicamba herbicide.
25. A method for producing soybean seed essentially free of toxic weed species seeds, said method
comprising:
a. planting seeds of soybean event MON 87708 in a field;
b. applying an effective dose of a dicamba herbicide to said field to kill toxic weeds in said field without injuring said soybean event MON 87708 plants; and
c. harvesting soybean seed from said field.
26. The method of claim 25, wherein said toxic weed species seeds are selected from the group consisting of Cardaria spp, Heliotropium spp, Centaurea spp., Senecio spp., Crotalaria spp., Solanum spp., Xanthium spp., Amsinckia spp., Cassia spp., Sesbania spp., Datura spp., Ricinus spp., Argemone spp., Corchorus spp., Impomoea spp., and Echium spp.
27. A method of producing a soybean plant that tolerates application of dicamba herbicide comprising: a. sexually crossing a transgenic soybean event MON 87708 plant comprising a nucleotide molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8, and complements thereof, with a second soybean plant;
b. collecting the seed produced from said cross;
c. growing said seed to produce a plurality of progeny plants;
d. treating said plurality of progeny plants with dicamba; and
e. selecting a progeny plant that is tolerant to dicamba.
28. A method of producing a soybean plant that tolerates application of dicamba herbicide comprising: a. selfing a transgenic soybean event MON 87708 plant comprising a nucleotide molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-8, and complements thereof;
b. collecting the seed produced from said selfing;
c. growing said seed to produce a plurality of progeny plants;
d. treating said plurality of progeny plants with dicamba; and
e. selecting a progeny plant that is tolerant to dicamba.
29. A method of determining the zygosity of a soybean event MON 87708 plant or seed comprising: a. contacting a sample comprising soybean DNA with a primer set comprising SEQ ID NO: 12, SEQ ID NO: 13, and SEQ ID NO: 14 and a probe set comprising SEQ ID NO: 15 and SEQ ID NO: 16;
b. performing a nucleic acid amplification reaction with said sample, primer set, and probe set; c. detecting in said nucleic acid amplification reaction a first fluorescent signal that is diagnostic for event MON 87708 and a second fluorescent signal different from said first fluorescent signal and diagnostic for native soybean genomic DNA corresponding to the location of insertion of the event MON 87708 transgene; and
d. analyzing the presence and/or absence of said first fluorescent signal and said second fluorescent signal in said nucleic acid amplification reaction, wherein the presence of both fluorescent signals indicates said sample is heterozygous for event MON 87708 and the presence of only said first fluorescent signal indicates said sample is homozygous for event MON 87708.
30. A soybean haplotype region on linkage group 9 at approximately map position 143.5 comprising a dicamba tolerance gene and further defined by haplotype window 19743 and 19767.
PCT/US2010/046759 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof WO2011034704A1 (en)

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CN2010800478901A CN102596984A (en) 2009-09-17 2010-08-26 Soybean transgenic event MON 87708 and methods of use thereof
NZ598724A NZ598724A (en) 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof
UAA201204680A UA115761C2 (en) 2009-09-17 2010-08-26 RECOMBINANT DNA MOLECULE INDICATING THE PRESENCE OF A TRANSGENIC OBJECT OF MON 87708
IN2963DEN2012 IN2012DN02963A (en) 2009-09-17 2010-08-26
AP2012006217A AP2872A (en) 2009-09-17 2010-08-26 Soybean transgenic event MON 87708 and methods of use thereof
EP10817646.2A EP2478000B1 (en) 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof
AU2010295864A AU2010295864C1 (en) 2009-09-17 2010-08-26 Soybean transgenic event MON 87708 and methods of use thereof
SG2012017455A SG179103A1 (en) 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof
ES10817646.2T ES2675311T3 (en) 2009-09-17 2010-08-26 GM 87708 soybean transgenic event and procedures for its use
KR1020127009679A KR101376028B1 (en) 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof
MX2014000571A MX351696B (en) 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof.
RU2012115127A RU2624025C2 (en) 2009-09-17 2010-08-26 Coi mon 87708 transgenic object and methods for its application
EP21154710.4A EP3875592A3 (en) 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof
BR112012006079-1A BR112012006079B1 (en) 2009-09-17 2010-08-26 Recombinant DNA molecule, methods and kit for its detection
MX2012003299A MX2012003299A (en) 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof.
JP2012529778A JP5726878B2 (en) 2009-09-17 2010-08-26 Soybean transgenic event MON87708 and method of use thereof
DK10817646.2T DK2478000T3 (en) 2009-09-17 2010-08-26 TRANSGEN SOYBEAN EVENT MON 87708 AND PROCEDURES FOR USING IT
CA2773929A CA2773929C (en) 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof
EP16171892.9A EP3127425B1 (en) 2009-09-17 2010-08-26 Soybean transgenic event mon 87708 and methods of use thereof

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WO2015138394A2 (en) 2014-03-11 2015-09-17 Bayer Cropscience Lp Hppd variants and methods of use
WO2015160620A1 (en) 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and an insecticide
WO2015160619A1 (en) 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and a fungicide
WO2015160618A1 (en) 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and a biological control agent
EP2997825A1 (en) 2011-04-22 2016-03-23 Bayer Intellectual Property GmbH Active compound combinations comprising a (thio)carboxamide derivative and a fungicidal compound
WO2016166077A1 (en) 2015-04-13 2016-10-20 Bayer Cropscience Aktiengesellschaft N-cycloalkyl-n-(biheterocyclyethylene)-(thio)carboxamide derivatives
EP3097782A1 (en) 2015-05-29 2016-11-30 Bayer CropScience Aktiengesellschaft Methods for controlling phytopathogenic nematodes by combination of fluopyram and biological control agents
WO2017042259A1 (en) 2015-09-11 2017-03-16 Bayer Cropscience Aktiengesellschaft Hppd variants and methods of use
EP3205210A1 (en) 2012-05-30 2017-08-16 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide selected from inhibitors of the succinate dehydrogenase
EP3243387A2 (en) 2012-05-30 2017-11-15 Bayer CropScience Aktiengesellschaft Compositions comprising a biological control agent and an insecticide
WO2018019676A1 (en) 2016-07-29 2018-02-01 Bayer Cropscience Aktiengesellschaft Active compound combinations and methods to protect the propagation material of plants
EP3281526A1 (en) 2012-05-30 2018-02-14 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
EP3292764A2 (en) 2012-05-30 2018-03-14 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide selected from inhibitors of the respiratory chain at complex iii
EP3300603A2 (en) 2012-05-30 2018-04-04 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
EP3318128A2 (en) 2012-05-30 2018-05-09 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
WO2018098214A1 (en) 2016-11-23 2018-05-31 Bayer Cropscience Lp Axmi669 and axmi991 toxin genes and methods for their use
WO2018119361A1 (en) 2016-12-22 2018-06-28 Bayer Cropscience Lp Elite event ee-gm4 and methods and kits for identifying such event in biological samples
WO2018114393A1 (en) 2016-12-19 2018-06-28 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018119336A1 (en) 2016-12-22 2018-06-28 Athenix Corp. Use of cry14 for the control of nematode pests
WO2018119364A1 (en) 2016-12-22 2018-06-28 Bayer Cropscience Lp Elite event ee-gm5 and methods and kits for identifying such event in biological samples
WO2018136604A1 (en) 2017-01-18 2018-07-26 Bayer Cropscience Lp Bp005 toxin gene and methods for its use
WO2018136611A1 (en) 2017-01-18 2018-07-26 Bayer Cropscience Lp Use of bp005 for the control of plant pathogens
EP3360418A1 (en) 2012-05-30 2018-08-15 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
EP3363289A2 (en) 2012-05-30 2018-08-22 Bayer CropScience Aktiengesellschaft Compositions comprising a biological control agent and an insecticide
WO2018153730A1 (en) 2017-02-21 2018-08-30 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018165091A1 (en) 2017-03-07 2018-09-13 Bayer Cropscience Lp Hppd variants and methods of use
WO2018184970A1 (en) 2017-04-07 2018-10-11 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018188962A1 (en) 2017-04-11 2018-10-18 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018195256A1 (en) 2017-04-21 2018-10-25 Bayer Cropscience Lp Method of improving crop safety
US10113178B2 (en) 2014-03-20 2018-10-30 Monsanto Technology Llc Transgenic maize event MON 87419 and methods of use thereof
WO2018202491A1 (en) 2017-05-04 2018-11-08 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2018202487A1 (en) 2017-05-04 2018-11-08 Basf Se Substituted 5-(haloalkyl)-5-hydroxy-isoxazoles for combating phytopathogenic fungi
WO2018219797A1 (en) 2017-06-02 2018-12-06 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018234139A1 (en) 2017-06-19 2018-12-27 Basf Se 2-[[5-(trifluoromethyl)-1,2,4-oxadiazol-3-yl]aryloxy](thio)acetamides for combating phytopathogenic fungi
WO2019025250A1 (en) 2017-08-04 2019-02-07 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019038042A1 (en) 2017-08-21 2019-02-28 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019052932A1 (en) 2017-09-18 2019-03-21 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019068811A1 (en) 2017-10-06 2019-04-11 Bayer Aktiengesellschaft Compositions comprising fluopyram and tioxazafen
WO2019083810A1 (en) 2017-10-24 2019-05-02 Basf Se Improvement of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (hppd) inhibitors by down-regulation of hppd expression in soybean
WO2019083808A1 (en) 2017-10-24 2019-05-02 Basf Se Improvement of herbicide tolerance to hppd inhibitors by down-regulation of putative 4-hydroxyphenylpyruvate reductases in soybean
WO2019101511A1 (en) 2017-11-23 2019-05-31 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019121143A1 (en) 2017-12-20 2019-06-27 Basf Se Substituted cyclopropyl derivatives
WO2019137995A1 (en) 2018-01-11 2019-07-18 Basf Se Novel pyridazine compounds for controlling invertebrate pests
WO2019145221A1 (en) 2018-01-29 2019-08-01 BASF Agro B.V. New agrochemical formulations
WO2019154663A1 (en) 2018-02-07 2019-08-15 Basf Se New pyridine carboxamides
WO2019154665A1 (en) 2018-02-07 2019-08-15 Basf Se New pyridine carboxamides
WO2019166257A1 (en) 2018-03-01 2019-09-06 BASF Agro B.V. Fungicidal compositions of mefentrifluconazole
WO2019219464A1 (en) 2018-05-15 2019-11-21 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019224092A1 (en) 2018-05-22 2019-11-28 Basf Se Pesticidally active c15-derivatives of ginkgolides
WO2019233863A1 (en) 2018-06-04 2019-12-12 Bayer Aktiengesellschaft Herbicidally active bicyclic benzoylpyrazoles
US10561083B2 (en) 2009-11-23 2020-02-18 Monsanto Technology Llc Transgenic maize event MON 87427 and the relative development scale
EP3613736A1 (en) 2018-08-22 2020-02-26 Basf Se Substituted glutarimide derivatives
EP3628158A1 (en) 2018-09-28 2020-04-01 Basf Se Pesticidal mixture comprising a mesoionic compound and a biopesticide
WO2020081464A1 (en) * 2018-10-16 2020-04-23 Monsanto Technology Llc Brassica event mon94100 and methods of use thereof
EP3643705A1 (en) 2018-10-24 2020-04-29 Basf Se Pesticidal compounds
WO2020083662A1 (en) 2018-10-23 2020-04-30 Basf Se Tricyclic pesticidal compounds
EP3670501A1 (en) 2018-12-17 2020-06-24 Basf Se Substituted [1,2,4]triazole compounds as fungicides
WO2020144308A1 (en) 2019-01-11 2020-07-16 Basf Se Crystalline forms of 1-(1,2-dimethylpropyl)-n-ethyl-5-methyl-n-pyridazin-4-yl-pyrazole-4-carboxamide
EP3696177A1 (en) 2019-02-12 2020-08-19 Basf Se Heterocyclic compounds for the control of invertebrate pests
EP3701796A1 (en) 2019-08-08 2020-09-02 Bayer AG Active compound combinations
EP3708565A1 (en) 2020-03-04 2020-09-16 Bayer AG Pyrimidinyloxyphenylamidines and the use thereof as fungicides
WO2020231751A1 (en) 2019-05-10 2020-11-19 Bayer Cropscience Lp Active compound combinations
WO2020239517A1 (en) 2019-05-29 2020-12-03 Basf Se Mesoionic imidazolium compounds and derivatives for combating animal pests
WO2020244968A1 (en) 2019-06-06 2020-12-10 Basf Se Fungicidal n-(pyrid-3-yl)carboxamides
WO2020244970A1 (en) 2019-06-06 2020-12-10 Basf Se New carbocyclic pyridine carboxamides
WO2020244969A1 (en) 2019-06-06 2020-12-10 Basf Se Pyridine derivatives and their use as fungicides
EP3766879A1 (en) 2019-07-19 2021-01-20 Basf Se Pesticidal pyrazole derivatives
EP3769623A1 (en) 2019-07-22 2021-01-27 Basf Se Mesoionic imidazolium compounds and derivatives for combating animal pests
WO2021013719A1 (en) 2019-07-23 2021-01-28 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021013721A1 (en) 2019-07-22 2021-01-28 Bayer Aktiengesellschaft 5-amino substituted pyrazoles and triazoles as pest control agents
WO2021013720A1 (en) 2019-07-23 2021-01-28 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021022069A1 (en) 2019-08-01 2021-02-04 Bayer Cropscience Lp Method of improving cold stress tolerance and crop safety
WO2021058659A1 (en) 2019-09-26 2021-04-01 Bayer Aktiengesellschaft Rnai-mediated pest control
WO2021063736A1 (en) 2019-10-02 2021-04-08 Basf Se Bicyclic pyridine derivatives
WO2021063735A1 (en) 2019-10-02 2021-04-08 Basf Se New bicyclic pyridine derivatives
WO2021064075A1 (en) 2019-10-02 2021-04-08 Bayer Aktiengesellschaft Active compound combinations comprising fatty acids
WO2021069567A1 (en) 2019-10-09 2021-04-15 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021069569A1 (en) 2019-10-09 2021-04-15 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021089673A1 (en) 2019-11-07 2021-05-14 Bayer Aktiengesellschaft Substituted sulfonyl amides for controlling animal pests
WO2021097162A1 (en) 2019-11-13 2021-05-20 Bayer Cropscience Lp Beneficial combinations with paenibacillus
WO2021099271A1 (en) 2019-11-18 2021-05-27 Bayer Aktiengesellschaft Active compound combinations comprising fatty acids
WO2021099303A1 (en) 2019-11-18 2021-05-27 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021105091A1 (en) 2019-11-25 2021-06-03 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
US11066424B2 (en) 2018-08-18 2021-07-20 Boragen, Inc. Solid forms of substituted benzoxaborole and compositions thereof
WO2021155084A1 (en) 2020-01-31 2021-08-05 Pairwise Plants Services, Inc. Suppression of shade avoidance response in plants
WO2021165195A1 (en) 2020-02-18 2021-08-26 Bayer Aktiengesellschaft Heteroaryl-triazole compounds as pesticides
WO2021211926A1 (en) 2020-04-16 2021-10-21 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
WO2021209490A1 (en) 2020-04-16 2021-10-21 Bayer Aktiengesellschaft Cyclaminephenylaminoquinolines as fungicides
WO2021213978A1 (en) 2020-04-21 2021-10-28 Bayer Aktiengesellschaft 2-(het)aryl-substituted condensed heterocyclic derivatives as pest control agents
EP3903583A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iii
EP3903584A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iv
EP3903582A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ii
EP3903581A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors i
WO2021219513A1 (en) 2020-04-28 2021-11-04 Basf Se Pesticidal compounds
WO2021224220A1 (en) 2020-05-06 2021-11-11 Bayer Aktiengesellschaft Pyridine (thio)amides as fungicidal compounds
WO2021224323A1 (en) 2020-05-06 2021-11-11 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
EP3909950A1 (en) 2020-05-13 2021-11-17 Basf Se Heterocyclic compounds for the control of invertebrate pests
WO2021228734A1 (en) 2020-05-12 2021-11-18 Bayer Aktiengesellschaft Triazine and pyrimidine (thio)amides as fungicidal compounds
WO2021233861A1 (en) 2020-05-19 2021-11-25 Bayer Aktiengesellschaft Azabicyclic(thio)amides as fungicidal compounds
EP3915971A1 (en) 2020-12-16 2021-12-01 Bayer Aktiengesellschaft Phenyl-s(o)n-phenylamidines and the use thereof as fungicides
WO2021245087A1 (en) 2020-06-04 2021-12-09 Bayer Aktiengesellschaft Heterocyclyl pyrimidines and triazines as novel fungicides
WO2021247477A1 (en) 2020-06-02 2021-12-09 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
WO2021249800A1 (en) 2020-06-10 2021-12-16 Basf Se Substituted [1,2,4]triazole compounds as fungicides
WO2021249995A1 (en) 2020-06-10 2021-12-16 Bayer Aktiengesellschaft Azabicyclyl-substituted heterocycles as fungicides
WO2021255089A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazole pyrimidines and 1,3,4-oxadiazole pyridines as fungicides
WO2021255170A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazole pyrimidines as fungicides
WO2021255169A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazole pyrimidines as fungicides
WO2021257775A1 (en) 2020-06-17 2021-12-23 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
WO2021255091A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazoles and their derivatives as fungicides
WO2021255118A1 (en) 2020-06-18 2021-12-23 Bayer Aktiengesellschaft Composition for use in agriculture
WO2021255071A1 (en) 2020-06-18 2021-12-23 Bayer Aktiengesellschaft 3-(pyridazin-4-yl)-5,6-dihydro-4h-1,2,4-oxadiazine derivatives as fungicides for crop protection
EP3929189A1 (en) 2020-06-25 2021-12-29 Bayer Animal Health GmbH Novel heteroaryl-substituted pyrazine derivatives as pesticides
WO2022002818A1 (en) 2020-07-02 2022-01-06 Bayer Aktiengesellschaft Heterocyclene derivatives as pest control agents
EP3939961A1 (en) 2020-07-16 2022-01-19 Basf Se Strobilurin type compounds and their use for combating phytopathogenic fungi
WO2022017836A1 (en) 2020-07-20 2022-01-27 BASF Agro B.V. Fungicidal compositions comprising (r)-2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1- (1,2,4-triazol-1-yl)propan-2-ol
EP3945089A1 (en) 2020-07-31 2022-02-02 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors v
WO2022033991A1 (en) 2020-08-13 2022-02-17 Bayer Aktiengesellschaft 5-amino substituted triazoles as pest control agents
EP3960727A1 (en) 2020-08-28 2022-03-02 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors vi
WO2022043559A2 (en) 2020-08-31 2022-03-03 Basf Se Yield improvement
WO2022053453A1 (en) 2020-09-09 2022-03-17 Bayer Aktiengesellschaft Azole carboxamide as pest control agents
EP3970494A1 (en) 2020-09-21 2022-03-23 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors viii
WO2022058327A1 (en) 2020-09-15 2022-03-24 Bayer Aktiengesellschaft Substituted ureas and derivatives as new antifungal agents
EP3974414A1 (en) 2020-09-25 2022-03-30 Bayer AG 5-amino substituted pyrazoles and triazoles as pesticides
WO2022089969A1 (en) 2020-10-27 2022-05-05 BASF Agro B.V. Compositions comprising mefentrifluconazole
WO2022090069A1 (en) 2020-11-02 2022-05-05 Basf Se Compositions comprising mefenpyr-diethyl
WO2022090071A1 (en) 2020-11-02 2022-05-05 Basf Se Use of mefenpyr-diethyl for controlling phytopathogenic fungi
WO2022106304A1 (en) 2020-11-23 2022-05-27 BASF Agro B.V. Compositions comprising mefentrifluconazole
WO2022129196A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft Heterobicycle substituted 1,2,4-oxadiazoles as fungicides
WO2022129190A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft (hetero)aryl substituted 1,2,4-oxadiazoles as fungicides
WO2022128524A1 (en) 2020-12-14 2022-06-23 Basf Se Sulfoximine pesticides
WO2022129200A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft Use of dhodh inhibitor for controlling resistant phytopathogenic fungi in crops
WO2022129188A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft 1,2,4-oxadiazol-3-yl pyrimidines as fungicides
EP4036083A1 (en) 2021-02-02 2022-08-03 Bayer Aktiengesellschaft 5-oxy substituted heterocycles as pesticides
EP4043444A1 (en) 2021-02-11 2022-08-17 Basf Se Substituted isoxazoline derivatives
WO2022173885A1 (en) 2021-02-11 2022-08-18 Pairwise Plants Services, Inc. Methods and compositions for modifying cytokinin oxidase levels in plants
WO2022182834A1 (en) 2021-02-25 2022-09-01 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture in plants
WO2022207496A1 (en) 2021-03-30 2022-10-06 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2022207494A1 (en) 2021-03-30 2022-10-06 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2022233758A1 (en) 2021-05-03 2022-11-10 Basf Se Additives for enhancing the pesticidal effectiveness of pesticidal microorganisms
WO2022233777A1 (en) 2021-05-06 2022-11-10 Bayer Aktiengesellschaft Alkylamide substituted, annulated imidazoles and use thereof as insecticides
WO2022238391A1 (en) 2021-05-12 2022-11-17 Bayer Aktiengesellschaft 2-(het)aryl-substituted condensed heterocycle derivatives as pest control agents
EP4091451A1 (en) 2021-05-17 2022-11-23 BASF Agro B.V. Compositions comprising mefentrifluconazole
WO2022243111A1 (en) 2021-05-18 2022-11-24 Basf Se New substituted pyridines as fungicides
WO2022243107A1 (en) 2021-05-18 2022-11-24 Basf Se New substituted pyridines as fungicides
WO2022243109A1 (en) 2021-05-18 2022-11-24 Basf Se New substituted quinolines as fungicides
WO2022266271A1 (en) 2021-06-17 2022-12-22 Pairwise Plants Services, Inc. Modification of growth regulating factor family transcription factors in soybean
WO2022263285A1 (en) 2021-06-14 2022-12-22 Basf Se Yield improvement by gene combinations
WO2022271892A1 (en) 2021-06-24 2022-12-29 Pairwise Plants Services, Inc. Modification of hect e3 ubiquitin ligase genes to improve yield traits
WO2023278651A1 (en) 2021-07-01 2023-01-05 Pairwise Plants Services, Inc. Methods and compositions for enhancing root system development
EP4119547A1 (en) 2021-07-12 2023-01-18 Basf Se Triazole compounds for the control of invertebrate pests
WO2023011958A1 (en) 2021-08-02 2023-02-09 Basf Se (3-pirydyl)-quinazoline
WO2023011957A1 (en) 2021-08-02 2023-02-09 Basf Se (3-quinolyl)-quinazoline
WO2023019188A1 (en) 2021-08-12 2023-02-16 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
WO2023017120A1 (en) 2021-08-13 2023-02-16 Bayer Aktiengesellschaft Active compound combinations and fungicide compositions comprising those
WO2023023496A1 (en) 2021-08-17 2023-02-23 Pairwise Plants Services, Inc. Methods and compositions for modifying cytokinin receptor histidine kinase genes in plants
EP4140986A1 (en) 2021-08-23 2023-03-01 Basf Se Pyrazine compounds for the control of invertebrate pests
EP4140995A1 (en) 2021-08-27 2023-03-01 Basf Se Pyrazine compounds for the control of invertebrate pests
WO2023025682A1 (en) 2021-08-25 2023-03-02 Bayer Aktiengesellschaft Novel pyrazinyl-triazole compounds as pesticides
EP4144739A1 (en) 2021-09-02 2023-03-08 Bayer Aktiengesellschaft Anellated pyrazoles as parasiticides
WO2023034731A1 (en) 2021-08-30 2023-03-09 Pairwise Plants Services, Inc. Modification of ubiquitin binding peptidase genes in plants for yield trait improvement
WO2023034891A1 (en) 2021-09-02 2023-03-09 Pairwise Plants Services, Inc. Methods and compositions for improving plant architecture and yield traits
EP4151631A1 (en) 2021-09-20 2023-03-22 Basf Se Heterocyclic compounds for the control of invertebrate pests
WO2023049720A1 (en) 2021-09-21 2023-03-30 Pairwise Plants Services, Inc. Methods and compositions for reducing pod shatter in canola
WO2023060152A2 (en) 2021-10-07 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023060028A1 (en) 2021-10-04 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023072671A1 (en) 2021-10-28 2023-05-04 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ix
WO2023072670A1 (en) 2021-10-28 2023-05-04 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors x
WO2023078915A1 (en) 2021-11-03 2023-05-11 Bayer Aktiengesellschaft Bis(hetero)aryl thioether (thio)amides as fungicidal compounds
WO2023099445A1 (en) 2021-11-30 2023-06-08 Bayer Aktiengesellschaft Bis(hetero)aryl thioether oxadiazines as fungicidal compounds
EP4194453A1 (en) 2021-12-08 2023-06-14 Basf Se Pyrazine compounds for the control of invertebrate pests
WO2023108035A1 (en) 2021-12-09 2023-06-15 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
EP4198033A1 (en) 2021-12-14 2023-06-21 Basf Se Heterocyclic compounds for the control of invertebrate pests
EP4198023A1 (en) 2021-12-16 2023-06-21 Basf Se Pesticidally active thiosemicarbazone compounds
WO2023147526A1 (en) 2022-01-31 2023-08-03 Pairwise Plants Services, Inc. Suppression of shade avoidance response in plants
WO2023148028A1 (en) 2022-02-01 2023-08-10 Globachem Nv Methods and compositions for controlling pests
WO2023148036A1 (en) 2022-02-01 2023-08-10 Globachem Nv Methods and compositions for controlling pests in soybean
WO2023156402A1 (en) 2022-02-17 2023-08-24 Basf Se Pesticidally active thiosemicarbazone compounds
WO2023156270A1 (en) 2022-02-18 2023-08-24 Basf Se Coumarin synthesis and uses thereof
RU2802940C2 (en) * 2019-03-28 2023-09-05 БАЙОСИРИЗ ЭлЭлСи Transgenic object ind-00410-5 of soya
EP4238971A1 (en) 2022-03-02 2023-09-06 Basf Se Substituted isoxazoline derivatives
WO2023168217A1 (en) 2022-03-02 2023-09-07 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
WO2023192838A1 (en) 2022-03-31 2023-10-05 Pairwise Plants Services, Inc. Early flowering rosaceae plants with improved characteristics
WO2023196886A1 (en) 2022-04-07 2023-10-12 Pairwise Plants Services, Inc. Methods and compositions for improving resistance to fusarium head blight
WO2023205714A1 (en) 2022-04-21 2023-10-26 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
WO2023213626A1 (en) 2022-05-03 2023-11-09 Bayer Aktiengesellschaft Use of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine for controlling unwanted microorganisms
WO2023215704A1 (en) 2022-05-02 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for enhancing yield and disease resistance
WO2023213670A1 (en) 2022-05-03 2023-11-09 Bayer Aktiengesellschaft Crystalline forms of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine
WO2023215809A1 (en) 2022-05-05 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture and/or improving plant yield traits
US11834466B2 (en) 2017-11-30 2023-12-05 5Metis, Inc. Benzoxaborole compounds and formulations thereof
EP4295688A1 (en) 2022-09-28 2023-12-27 Bayer Aktiengesellschaft Active compound combination
WO2024006791A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024006792A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024006679A1 (en) 2022-06-27 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
WO2024018016A1 (en) 2022-07-21 2024-01-25 Syngenta Crop Protection Ag Crystalline forms of 1,2,4-oxadiazole fungicides
WO2024030984A1 (en) 2022-08-04 2024-02-08 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
WO2024028243A1 (en) 2022-08-02 2024-02-08 Basf Se Pyrazolo pesticidal compounds
WO2024036240A1 (en) 2022-08-11 2024-02-15 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024033374A1 (en) 2022-08-11 2024-02-15 Syngenta Crop Protection Ag Novel arylcarboxamide or arylthioamide compounds
WO2024054880A1 (en) 2022-09-08 2024-03-14 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
EP4342885A1 (en) 2022-09-20 2024-03-27 Basf Se N-(3-(aminomethyl)-phenyl)-5-(4-phenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-amine derivatives and similar compounds as pesticides
WO2024068837A1 (en) 2022-09-28 2024-04-04 Syngenta Crop Protection Ag Agricultural methods
WO2024068517A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2024068838A1 (en) 2022-09-28 2024-04-04 Syngenta Crop Protection Ag Fungicidal compositions
WO2024068519A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2024068518A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-heteroaryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2024068520A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
EP4361126A1 (en) 2022-10-24 2024-05-01 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors xv
WO2024100069A1 (en) 2022-11-08 2024-05-16 Syngenta Crop Protection Ag Microbiocidal pyridine derivatives
WO2024104823A1 (en) 2022-11-16 2024-05-23 Basf Se New substituted tetrahydrobenzoxazepine
WO2024104822A1 (en) 2022-11-16 2024-05-23 Basf Se Substituted tetrahydrobenzodiazepine as fungicides
WO2024104815A1 (en) 2022-11-16 2024-05-23 Basf Se Substituted benzodiazepines as fungicides
WO2024104818A1 (en) 2022-11-16 2024-05-23 Basf Se Substituted benzodiazepines as fungicides
EP4385327A1 (en) 2022-12-15 2024-06-19 Kimitec Group S.L. Biopesticide composition and method for controlling and treating broad spectrum of pests and diseases in plants
EP4389210A1 (en) 2022-12-21 2024-06-26 Basf Se Heteroaryl compounds for the control of invertebrate pests
WO2024137438A2 (en) 2022-12-19 2024-06-27 BASF Agricultural Solutions Seed US LLC Insect toxin genes and methods for their use
WO2024165343A1 (en) 2023-02-08 2024-08-15 Basf Se New substituted quinoline compounds for combatitng phytopathogenic fungi
WO2024173622A1 (en) 2023-02-16 2024-08-22 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
WO2024182658A1 (en) 2023-03-02 2024-09-06 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
WO2024186950A1 (en) 2023-03-09 2024-09-12 Pairwise Plants Services, Inc. Modification of brassinosteroid signaling pathway genes for improving yield traits in plants
WO2024194038A1 (en) 2023-03-17 2024-09-26 Basf Se Substituted pyridyl/pyrazidyl dihydrobenzothiazepine compounds for combatting phytopathogenic fungi
EP4455137A1 (en) 2023-04-24 2024-10-30 Basf Se Pyrimidine compounds for the control of invertebrate pests

Families Citing this family (1029)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0908809A2 (en) 2008-02-15 2015-08-18 Monsanto Technology Llc Soybean and seed plant corresponding to transgenic event mon87769 and methods for its detection
PT2478000T (en) 2009-09-17 2018-07-17 Monsanto Technology Llc Soybean transgenic event mon 87708 and methods of use thereof
PT2575431T (en) 2010-06-04 2018-06-21 Monsanto Technology Llc Transgenic brassica event mon 88302 and methods of use thereof
TWI667347B (en) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 Soybean event syht0h2 and compositions and methods for detection thereof
AR086540A1 (en) * 2011-05-24 2014-01-08 Basf Plant Science Co Gmbh PHYTOPHTHORA RESISTANT POPE DEVELOPMENT WITH GREATER PERFORMANCE
UA117816C2 (en) 2012-11-06 2018-10-10 Байєр Кропсайєнс Акцієнгезелльшафт Herbicidal combinations for tolerant soybean cultures
US9408359B1 (en) 2013-08-29 2016-08-09 Monsanto Technology Llc Soybean variety XB36G13
US9332707B2 (en) 2013-08-29 2016-05-10 Monsanto Technology Llc Soybean variety XB04F13
CA2861217C (en) 2013-08-29 2022-07-19 Monsanto Technology Llc Soybean variety xb007g13
US9271462B1 (en) 2013-08-29 2016-03-01 Monsanto Technology Llc Soybean variety XB49AE13
US9485930B1 (en) 2013-08-29 2016-11-08 Monsanto Technology Llc Soybean variety XB35W13
US9480226B1 (en) 2013-08-29 2016-11-01 Monsanto Technology Llc Soybean variety XB34Z13
US9332712B2 (en) 2013-08-29 2016-05-10 Monsanto Technology Llc Soybean variety XB28T13
US9433175B2 (en) 2013-08-29 2016-09-06 Monsanto Technology Llc Soybean variety XB33Z13
CA2861228A1 (en) 2013-08-29 2015-02-28 Monsanto Technology Llc Soybean variety xb09ae13
CA2861213A1 (en) 2013-08-29 2015-02-28 Monsanto Technology Llc Soybean variety xb21z13
CA2861216A1 (en) 2013-08-29 2015-02-28 Monsanto Technology Llc Soybean variety xb22a13
US9485932B1 (en) 2013-08-29 2016-11-08 Monsanto Technology Llc Soybean variety XB40U13
CA2861223A1 (en) 2013-08-29 2015-02-28 Monsanto Technology Llc Soybean variety xb23ab13
US9271464B1 (en) 2013-08-29 2016-03-01 Monsanto Technology Llc Soybean variety XB35Y13
US9271463B1 (en) 2013-08-29 2016-03-01 Monsanto Technology Llc Soybean variety XB56V13
US9408358B1 (en) 2013-08-29 2016-08-09 Monsanto Technology Llc Soybean variety XB34Y13
US9445567B1 (en) 2013-08-29 2016-09-20 Monsanto Technology Llc Soybean variety XB39D13
US9408357B1 (en) 2013-08-29 2016-08-09 Monsanto Technology Llc Soybean variety XB35X13
US9320233B1 (en) 2013-08-29 2016-04-26 Monsanto Technology Llc Soybean variety XB55B13
US9485931B1 (en) 2013-08-29 2016-11-08 Monsanto Technology Llc Soybean variety XB39C13
US9332713B1 (en) 2013-08-29 2016-05-10 Monsanto Technology Llc Soybean variety XB44K13
US9497914B2 (en) 2013-12-12 2016-11-22 Monsanto Technology Llc Soybean variety 01045730
US9554528B2 (en) 2013-12-13 2017-01-31 Monsanto Technology Llc Soybean variety 01045954
US9357731B2 (en) 2013-12-13 2016-06-07 Monsanto Technology Llc Soybean variety 01046948
US9554529B2 (en) 2013-12-13 2017-01-31 Monsanto Technology Llc Soybean variety 01046035
US9370155B2 (en) 2013-12-16 2016-06-21 Monsanto Technology Llc Soybean variety 01046182
US9386754B2 (en) 2013-12-17 2016-07-12 Monsanto Technology, Llc Soybean variety 01046551
CA2875106A1 (en) 2013-12-17 2015-06-17 Monsanto Technology Llc Soybean variety 01046426
US9445568B1 (en) 2013-12-17 2016-09-20 Monsanto Technology Llc Soybean variety 01046626
US9370157B2 (en) 2013-12-17 2016-06-21 Monsanto Technology Llc Soybean variety 01046703
US9345217B2 (en) 2013-12-17 2016-05-24 Monsanto Technology Llc Soybean variety 01046814
CN103725777B (en) * 2013-12-18 2015-07-15 南京佳邦食品有限公司 Real-time fluorescence PCR (Polymerase Chain Reaction) method for rapidly detecting transgenic soybean MON89788
US9363967B2 (en) 2013-12-18 2016-06-14 Monsanto Technology Llc Soybean variety 01046942
US9370156B2 (en) 2013-12-18 2016-06-21 Monsanto Technology Llc Soybean variety 01046863
US9357732B2 (en) 2013-12-18 2016-06-07 Monsanto Technology Llc Soybean variety 01046898
US9521816B2 (en) 2014-05-08 2016-12-20 Monsanto Technology Llc Soybean variety 01051547
US9363973B2 (en) 2014-05-08 2016-06-14 Monsanto Technology Llc Soybean variety 01051540
US9363974B2 (en) 2014-05-08 2016-06-14 Monsanto Technology Llc Soybean variety 01051545
US9345224B2 (en) 2014-05-08 2016-05-24 Monsanto Technology Llc Soybean variety 01051076
US9510544B2 (en) 2014-05-08 2016-12-06 Monsanto Technology Llc Soybean variety 01050893
CA2890604A1 (en) 2014-05-08 2015-11-08 Monsanto Technology Llc Soybean variety 01050938
US9510545B2 (en) 2014-05-08 2016-12-06 Monsanto Technology Llc Soybean variety 01050923
US9655320B2 (en) 2014-05-09 2017-05-23 Monsanto Technology Llc Soybean variety 01051581
US9345225B2 (en) 2014-05-09 2016-05-24 Monsanto Technology Llc Soybean variety 01051559
US9655322B2 (en) 2014-05-09 2017-05-23 Monsanto Technology Llc Soybean variety 01051603
US9655321B2 (en) 2014-05-09 2017-05-23 Monsanto Technology Llc Soybean variety 01051585
US9585347B2 (en) 2014-09-29 2017-03-07 Monsanto Technology Llc Soybean variety 01046668
US9320243B1 (en) 2014-11-19 2016-04-26 Monsanto Technology Llc Soybean variety XR45Z14RX
US9675026B1 (en) 2014-11-19 2017-06-13 Monsanto Technology Llc Soybean variety XR35AP14RX
US9320239B1 (en) 2014-11-19 2016-04-26 Monsanto Technology Llc Soybean variety XR35AR14RX
US9332717B1 (en) 2014-11-19 2016-05-10 Monsanto Technology Llc Soybean variety XR68D14RX
US9648819B1 (en) 2014-11-19 2017-05-16 Monsanto Technology Llc Soybean variety XR57F14RX
US9554538B2 (en) 2014-11-19 2017-01-31 Monsanto Technology Llc Soybean variety XR27AK14RX
US9585321B1 (en) 2014-11-19 2017-03-07 Monsanto Technology Llc Soybean variety XR34AT14RX
CA2883600C (en) 2014-11-19 2017-08-29 Monsanto Technology Llc Soybean variety xr30ar14rx
US9554539B2 (en) 2014-11-19 2017-01-31 Monsanto Technology Llc Soybean variety XR30AT14RX
US9402362B1 (en) 2014-11-19 2016-08-02 Monsanto Technology Llc Soybean variety XR47AB14RX
US9320240B1 (en) 2014-11-19 2016-04-26 Monsanto Technology Llc Soybean variety XR35AS14RX
US9320242B1 (en) 2014-11-19 2016-04-26 Monsanto Technology Llc Soybean variety XR43A14RX
US9320241B1 (en) 2014-11-19 2016-04-26 Monsanto Technology Llc Soybean variety XR37Z14RX
US9433180B1 (en) 2014-11-24 2016-09-06 Monsanto Technology Llc Soybean variety XB35AL14R2
US9332716B1 (en) 2014-11-24 2016-05-10 Monsanto Technology LC Soybean variety XB42A14R2
US9414562B1 (en) 2014-11-24 2016-08-16 Monsanto Technology Llc Soybean variety XB47AA14R2
US9554537B2 (en) 2014-11-24 2017-01-31 Monsanto Technology Llc Soybean variety XB07A14R2
US9648818B1 (en) 2014-11-24 2017-05-16 Monsanto Technology Llc Soybean variety XB37X14R2
US9271470B1 (en) 2014-11-24 2016-03-01 Monsanto Technology Llc Soybean variety XB69F14R2
US9504222B2 (en) 2014-11-24 2016-11-29 Monsanto Technology Llc Soybean variety XB15R14R2
US9635819B1 (en) 2014-11-24 2017-05-02 Monsanto Technology Llc Soybean variety XB57E14R2
US9320238B1 (en) 2014-11-24 2016-04-26 Monsanto Technology Llc Soybean variety XB20AA14R2
US9351467B1 (en) 2015-03-02 2016-05-31 Monsanto Technology Llc Soybean variety XB32AQ13R2
US9609826B2 (en) 2015-05-20 2017-04-04 Monsanto Technology Llc Soybean variety 01050982
US9585336B2 (en) 2015-05-20 2017-03-07 Monsanto Technology Llc Soybean variety 01059528
US9585335B2 (en) 2015-05-20 2017-03-07 Monsanto Technology Llc Soybean variety 01050939
US9585332B2 (en) 2015-05-20 2017-03-07 Monsanto Technology Llc Soybean variety 01059874
US9585331B2 (en) 2015-05-20 2017-03-07 Monsanto Technology Llc Soybean variety 01059966
US9615525B2 (en) 2015-05-20 2017-04-11 Monsanto Technology Llc Soybean variety 01051642
US9585334B2 (en) 2015-05-20 2017-03-07 Monsanto Technology Llc Soybean variety 01059962
US9585333B2 (en) 2015-05-20 2017-03-07 Monsanto Technology Llc Soybean variety 01059967
US9788503B2 (en) 2015-05-21 2017-10-17 Monsanto Technology Llc Soybean variety 01051964
US9491920B1 (en) 2015-05-21 2016-11-15 Monsanto Technology Llc Soybean variety 01050862
US9439384B1 (en) 2015-05-21 2016-09-13 Monsanto Technology Llc Soybean variety 01051734
US9456577B1 (en) 2015-05-21 2016-10-04 Monsanto Technology Llc Soybean variety 01051789
US9474238B1 (en) 2015-05-21 2016-10-25 Monsanto Technology Llc Soybean variety 01050800
US9781891B2 (en) 2015-05-21 2017-10-10 Monsanto Technology Llc Soybean variety 01051941
US9609828B2 (en) 2015-05-21 2017-04-04 Monsanto Technology Llc Soybean variety 01051807
US9439383B1 (en) 2015-05-21 2016-09-13 Monsanto Technology Llc Soybean variety 01051693
US9439382B1 (en) 2015-05-21 2016-09-13 Monsanto Technology Llc Soybean variety 01050895
US9609827B2 (en) 2015-05-21 2017-04-04 Monsanto Technology Llc Soybean variety 01050829
US9655324B2 (en) 2015-05-22 2017-05-23 Monsanto Technology Llc Soybean variety 01051871
US9468180B1 (en) 2015-05-22 2016-10-18 Monsanto Technology Llc Soybean variety 01051028
US9497922B1 (en) 2015-05-22 2016-11-22 Monsanto Technology Llc Soybean variety 01051809
US9510547B1 (en) 2015-05-22 2016-12-06 Monsanto Technology Llc Soybean variety 01051813
US9497920B1 (en) 2015-05-22 2016-11-22 Monsanto Technology Llc Soybean variety 01051652
US9655323B2 (en) 2015-05-22 2017-05-23 Monsanto Technology Llc Soybean variety 01050561
US9468179B1 (en) 2015-05-22 2016-10-18 Monsanto Technology Llc Soybean variety 01050806
US9510546B1 (en) 2015-05-22 2016-12-06 Monsanto Technology Llc Soybean variety 01050728
US9585338B2 (en) 2015-05-22 2017-03-07 Monsanto Technology Llc Soybean variety 01051129
US9585337B2 (en) 2015-05-22 2017-03-07 Monsanto Technology Llc Soybean variety 01051092
US9622442B2 (en) 2015-05-22 2017-04-18 Monsanto Technology Llc Soybean variety 01050576
US9462778B1 (en) 2015-05-22 2016-10-11 Monsanto Technology Llc Soybean variety 01050913
US9693514B2 (en) 2015-05-22 2017-07-04 Monsanto Technology Llc Soybean variety 01051868
US9497921B1 (en) 2015-05-22 2016-11-22 Monsanto Technology Llc Soybean variety 01051779
US9585339B2 (en) 2015-05-28 2017-03-07 Monsanto Technology Llc Soybean variety 01050639
US9521823B1 (en) 2015-05-28 2016-12-20 Monsanto Technology Llc Soybean variety 01051901
US9521822B1 (en) 2015-05-28 2016-12-20 Monsanto Technology Llc Soybean variety 01051937
US9474239B1 (en) 2015-05-28 2016-10-25 Monsanto Technology Llc Soybean variety 01051127
US9585340B2 (en) 2015-05-28 2017-03-07 Monsanto Technology Llc Soybean variety 01052124
US9648825B2 (en) 2015-05-28 2017-05-16 Monsanto Technology Llc Soybean variety 01052098
US9655327B2 (en) 2015-05-28 2017-05-23 Monsanto Technology Llc Soybean variety 01051842
US9675028B2 (en) 2015-05-28 2017-06-13 Monsanto Technology Llc Soybean variety 01051998
US9585341B2 (en) 2015-05-28 2017-03-07 Monsanto Technology Llc Soybean variety 01052100
US9578842B2 (en) 2015-05-28 2017-02-28 Monsanto Technology Llc Soybean variety 01052083
US9655326B2 (en) 2015-05-28 2017-05-23 Monsanto Technology Llc Soybean variety 01051843
US9510548B1 (en) 2015-05-28 2016-12-06 Monsanto Technology Llc Soybean variety 01052178
US9655325B2 (en) 2015-05-28 2017-05-23 Monsanto Technology Llc Soybean variety 01050591
US9655328B2 (en) 2015-05-28 2017-05-23 Monsanto Technology Llc Soybean variety 01052052
US9521821B1 (en) 2015-05-28 2016-12-20 Monsanto Technology Llc Soybean variety 01051938
US9585343B2 (en) 2015-06-03 2017-03-07 Monsanto Technology Llc Soybean variety 01050761
US9510549B1 (en) 2015-06-03 2016-12-06 Monsanto Technology Llc Soybean variety 01050689
US9532543B2 (en) 2015-06-03 2017-01-03 Monsanto Technology Llc Soybean variety 01050742
US9675029B2 (en) 2015-06-03 2017-06-13 Monsanto Technology Llc Soybean variety 01050834
US9609829B2 (en) 2015-06-03 2017-04-04 Monsanto Technology Llc Soybean variety 01050652
US9532544B2 (en) 2015-06-03 2017-01-03 Monsanto Technology Llc Soybean variety 01050668
US9510551B1 (en) 2015-06-03 2016-12-06 Monsanto Technology Llc Soybean variety 01050909
US9675030B2 (en) 2015-06-03 2017-06-13 Monsanto Technology Llc Soybean variety 01050762
US9585342B2 (en) 2015-06-03 2017-03-07 Monsanto Technology Llc Soybean variety 01050585
US9510550B1 (en) 2015-06-03 2016-12-06 Monsanto Technology Llc Soybean variety 01050509
US9521824B1 (en) 2015-06-03 2016-12-20 Monsanto Technology Llc Soybean variety 01050628
US10104848B2 (en) 2015-06-25 2018-10-23 Monsanto Technology Llc Soybean variety 01051773
US9781897B2 (en) 2015-06-25 2017-10-10 Monsanto Technology Llc Soybean variety 01058741
US9788516B2 (en) 2015-06-25 2017-10-17 Monsanto Technology Llc Soybean variety 01058627
US9743630B2 (en) 2015-06-25 2017-08-29 Monsanto Technology Llc Soybean variety 01058664
US9832963B2 (en) 2015-06-25 2017-12-05 Monsanto Technology Llc Soybean variety 01058403
US9814206B2 (en) 2015-06-25 2017-11-14 Monsanto Technology Llc Soybean variety 01051877
US9907262B2 (en) 2015-06-25 2018-03-06 Monsanto Technology Llc Soybean variety 01051117
US9832964B2 (en) 2015-06-25 2017-12-05 Monsanto Technology Llc Soybean variety 01051705
US9986704B2 (en) 2015-06-25 2018-06-05 Monsanto Technology Llc Soybean variety 01057162
US10076093B2 (en) 2015-06-25 2018-09-18 Monsanto Technology Llc Soybean variety 01058524
US9826701B2 (en) 2015-06-25 2017-11-28 Monsanto Technology Llc Soybean variety 01050769
US9743631B2 (en) 2015-06-25 2017-08-29 Monsanto Technology Llc Soybean variety 01058733
US9974268B2 (en) 2015-06-25 2018-05-22 Monsanto Technology Llc Soybean variety 01051074
US9781898B2 (en) 2015-06-25 2017-10-10 Monsanto Technology Llc Soybean variety 01058771
US9807961B2 (en) 2015-06-25 2017-11-07 Monsanto Technology Llc Soybean variety 01058472
US9807962B2 (en) 2015-06-25 2017-11-07 Monsanto Technology Llc Soybean variety 01058613
US9775320B2 (en) 2015-06-25 2017-10-03 Monsanto Technology Llc Soybean variety 01058744
US9814205B2 (en) 2015-06-25 2017-11-14 Monsanto Technology Llc Soybean variety 01058730
US9894860B2 (en) 2015-06-25 2018-02-20 Monsanto Technology Llc Soybean variety 01058780
US9924677B2 (en) 2015-06-28 2018-03-27 Monsanto Technology Llc Soybean variety 01052136
US9907265B2 (en) 2015-06-28 2018-03-06 Monsanto Technology Llc Soybean variety 01058380
US9844200B2 (en) 2015-06-28 2017-12-19 Monsanto Technology Llc Soybean variety 01058749
US9924676B2 (en) 2015-06-28 2018-03-27 Monsanto Technology Llc Soybean variety 01057057
US9826703B2 (en) 2015-06-28 2017-11-28 Monsanto Technology Llc Soybean variety 01058683
US9807963B2 (en) 2015-06-28 2017-11-07 Monsanto Technology Llc Soybean variety 01052094
US9844199B2 (en) 2015-06-28 2017-12-19 Monsanto Technology Llc Soybean variety 01058475
US10051807B2 (en) 2015-06-28 2018-08-21 Monsanto Technology Llc Soybean variety 01058772
US9820458B2 (en) 2015-06-28 2017-11-21 Monsanto Technology Llc Soybean variety 01052110
US9848569B2 (en) 2015-06-28 2017-12-26 Monsanto Technology Llc Soybean variety 01057209
US9907266B2 (en) 2015-06-28 2018-03-06 Monsanto Technology Llc Soybean variety 01057255
US9974269B2 (en) 2015-06-28 2018-05-22 Monsanto Technology Llc Soybean variety 01058766
US9924679B2 (en) 2015-06-29 2018-03-27 Monsanto Technology Llc Soybean variety 01057010
US9907271B2 (en) 2015-06-29 2018-03-06 Monsanto Technology Llc Soybean variety 01056960
US9907267B2 (en) 2015-06-29 2018-03-06 Monsanto Technology Llc Soybean variety 01057383
US9924678B2 (en) 2015-06-29 2018-03-27 Monsanto Technology Llc Soybean variety 01057325
US10034445B2 (en) 2015-06-29 2018-07-31 Monsanto Technology Llc Soybean variety 01050611
US9907269B2 (en) 2015-06-29 2018-03-06 Monsanto Technology Llc Soybean variety 01050536
US9907270B2 (en) 2015-06-29 2018-03-06 Monsanto Technology Llc Soybean variety 01057497
US9901057B2 (en) 2015-06-29 2018-02-27 Monsanto Technology Llc Soybean variety 01050566
US9907268B2 (en) 2015-06-29 2018-03-06 Monsanto Technology Llc Soybean variety 01057333
US9844201B2 (en) 2015-06-29 2017-12-19 Monsanto Technology Llc Soybean variety 01050745
US9968057B2 (en) 2015-06-29 2018-05-15 Monsanto Technology Llc Soybean variety 01058336
US9901056B2 (en) 2015-06-29 2018-02-27 Monsanto Technology Llc Soybean variety 01057377
US10034444B2 (en) 2015-06-30 2018-07-31 Monsanto Technology Llc Soybean variety 01050609
US9901055B2 (en) 2015-06-30 2018-02-27 Monsanto Technology Llc Soybean variety 01050596
US9826702B2 (en) 2015-06-30 2017-11-28 Monsanto Technology Llc Soybean variety 01051835
US10004197B2 (en) 2015-06-30 2018-06-26 Monsanto Technology Llc Soybean variety 01050595
US9832965B2 (en) 2015-06-30 2017-12-05 Monsanto Technology Llc Soybean variety 01051604
US10070606B2 (en) 2015-06-30 2018-09-11 Monsanto Technology Llc Soybean variety 01058789
US9820459B2 (en) 2015-06-30 2017-11-21 Monsanto Technology Llc Soybean variety 01058808
US9907263B2 (en) 2015-06-30 2018-03-06 Monsanto Technology Llc Soybean variety 01050612
US9814207B2 (en) 2015-06-30 2017-11-14 Monsanto Technology Llc Soybean variety 01058565
US9907264B2 (en) 2015-06-30 2018-03-06 Monsanto Technology Llc Soybean variety 01052085
US9258973B1 (en) 2015-08-05 2016-02-16 Monsanto Technology Llc Soybean cultivar 46092117
US9351470B1 (en) 2015-08-05 2016-05-31 Monsanto Technology Llc Soybean cultivar 46043017
US9351471B1 (en) 2015-08-05 2016-05-31 Monsanto Technology Llc Soybean cultivar 46434117
CN105002291B (en) * 2015-08-10 2018-01-12 吉林省农业科学院 Genetically engineered soybean MON87708 LAMP detection primer group, kit and detection method
US9901059B2 (en) 2015-09-24 2018-02-27 Monsanto Technology Llc Soybean variety 01051956
US9907273B2 (en) 2015-09-24 2018-03-06 Monsanto Technology Llc Soybean variety 01051571
US9901060B2 (en) 2015-09-24 2018-02-27 Monsanto Technology Llc Soybean variety 01051946
US9901062B2 (en) 2015-09-24 2018-02-27 Monsanto Technology Llc Soybean variety 01052029
US9894862B2 (en) 2015-09-24 2018-02-20 Monsanto Technology Llc Soybean variety 01058666
US9907274B2 (en) 2015-09-24 2018-03-06 Monsanto Technology Llc Soybean variety 01052030
US9901061B2 (en) 2015-09-24 2018-02-27 Monsanto Technology Llc Soybean variety 01052008
US9629325B1 (en) 2015-12-01 2017-04-25 Monsanto Technology Llc Soybean variety 01057518
CN105483239A (en) * 2015-12-26 2016-04-13 吉林省农业科学院 LAMP (Loop-mediated isothermal amplification) detection primer group and kit and detection method for gene dmo of transgenic plants
US9596819B1 (en) 2016-03-21 2017-03-21 Monsanto Technology Llc Soybean variety XR33AU15X
US9700000B1 (en) 2016-03-21 2017-07-11 Monsanto Technology Llc Soybean variety XR38W15X
US9763412B1 (en) 2016-03-21 2017-09-19 Monsanto Technology Llc Soybean variety XR09AP15X
US9585354B1 (en) 2016-03-21 2017-03-07 Monsanto Technology Llc Soybean variety XR48C15X
US9603331B1 (en) 2016-03-21 2017-03-28 Monsanto Technology Llc Soybean variety XR39V15X
US9861056B1 (en) 2016-03-21 2018-01-09 Monsanto Technology Llc Soybean variety XR56J15X
US9699999B1 (en) 2016-03-21 2017-07-11 Monsanto Technology Llc Soybean variety XR22K15X
US9763411B1 (en) 2016-03-21 2017-09-19 Monsanto Technology Llc Soybean variety XR09AL15X
US9560829B1 (en) 2016-03-21 2017-02-07 Monsanto Technology Llc Soybean variety XR47AG15X
US9565821B1 (en) 2016-03-21 2017-02-14 Monsanto Technology Llc Soybean variety XR47AF15X
US9861055B1 (en) 2016-03-21 2018-01-09 Monsanto Technology Llc Soybean variety XR39U15X
US9560828B1 (en) 2016-03-21 2017-02-07 Monsanto Technology Llc Soybean variety XR46Z15X
US9788508B1 (en) 2016-03-21 2017-10-17 Monsanto Technology Llc Soybean variety XR20AH15X
US9565822B1 (en) 2016-03-21 2017-02-14 Monsanto Technology Llc Soybean variety XR37AE15X
US9560827B1 (en) 2016-03-21 2017-02-07 Monsanto Technology Llc Soybean variety XR45C15X
US9763413B1 (en) 2016-03-21 2017-09-19 Monsanto Technology Llc Soybean variety XR23AQ15X
US9730415B1 (en) 2016-03-21 2017-08-15 Monsanto Technology Llc Soybean variety XR08A15X
US9596818B1 (en) 2016-03-21 2017-03-21 Monsanto Technology Llc Soybean variety XR30AX15X
US9861053B1 (en) 2016-03-21 2018-01-09 Monsanto Technology Llc Soybean variety XR36AC15X
US9861054B1 (en) 2016-03-21 2018-01-09 Monsanto Technology Llc Soybean variety XR36Z15X
US9585355B1 (en) 2016-03-21 2017-03-07 Monsanto Technology Llc Soybean variety XR40AA15X
US10010040B1 (en) 2016-03-21 2018-07-03 Monsanto Technology Llc Soybean variety XR50E15X
US9763414B1 (en) 2016-03-21 2017-09-19 Monsanto Technology Llc Soybean variety XR25AL15X
US9655336B1 (en) * 2016-03-22 2017-05-23 Monsanto Technology Llc Soybean cultivar 58150703
US9648836B1 (en) * 2016-03-22 2017-05-16 Monsanto Technology Llc Soybean cultivar 50262411
US9648835B1 (en) * 2016-03-22 2017-05-16 Monsanto Technology Llc Soybean cultivar 53030521
US9907246B2 (en) 2016-05-31 2018-03-06 Monsanto Technology Llc Soybean variety 01056904
US9907245B2 (en) 2016-05-31 2018-03-06 Monsanto Technology Llc Soybean variety 01056902
US9888646B2 (en) 2016-05-31 2018-02-13 Monsanto Technology Llc Soybean variety 01057021
US9743623B1 (en) 2016-05-31 2017-08-29 Monsanto Technology Llc Soybean variety 01056977
US9901051B2 (en) 2016-05-31 2018-02-27 Monsanto Technology Llc Soybean variety 01057075
US9924672B2 (en) 2016-05-31 2018-03-27 Monsanto Technology Llc Soybean variety 01056984
US9877450B2 (en) 2016-05-31 2018-01-30 Monsanto Technology Llc Soybean variety 01056979
US9888648B2 (en) 2016-05-31 2018-02-13 Monsanto Technology Llc Soybean variety 01057115
US9750218B1 (en) 2016-05-31 2017-09-05 Monsanto Technology Llc Soybean variety 01056968
US9756823B1 (en) 2016-05-31 2017-09-12 Monsanto Technology Llc Soybean variety 01056961
US9888647B2 (en) 2016-05-31 2018-02-13 Monsanto Technology Llc Soybean variety 01057072
US9743624B1 (en) 2016-05-31 2017-08-29 Monsanto Technology Llc Soybean variety 01056994
US9867351B2 (en) 2016-05-31 2018-01-16 Monsanto Technology Llc Soybean variety 01057076
US9907247B2 (en) 2016-05-31 2018-03-06 Monsanto Technology Llc Soybean variety 01056929
US9713311B1 (en) 2016-05-31 2017-07-25 Monsanto Technology Llc Soybean variety 01057181
US10051806B2 (en) 2016-05-31 2018-08-21 Monsanto Technology Llc Soybean variety 01057079
US10226001B2 (en) 2016-05-31 2019-03-12 Monsanto Technology Llc Soybean variety 01056938
US9872469B2 (en) 2016-05-31 2018-01-23 Monsanto Technology Llc Soybean variety 01056991
US9901052B2 (en) 2016-05-31 2018-02-27 Monsanto Technology Llc Soybean variety 01057176
US9867352B2 (en) 2016-05-31 2018-01-16 Monsanto Technology Llc Soybean variety 01057081
US9907251B2 (en) 2016-06-01 2018-03-06 Monsanto Technology Llc Soybean variety 01058313
US9700008B1 (en) 2016-06-01 2017-07-11 Monsanto Technology Llc Soybean variety 01057341
US9907252B2 (en) 2016-06-01 2018-03-06 Monsanto Technology Llc Soybean variety 01058320
US9788515B1 (en) 2016-06-01 2017-10-17 Monsanto Technology Llc Soybean variety 01057347
US9854772B2 (en) 2016-06-01 2018-01-02 Monsanto Technology Llc Soybean variety 01058360
US9907249B2 (en) 2016-06-01 2018-03-06 Monsanto Technology Llc Soybean variety 01057459
US9848563B1 (en) 2016-06-01 2017-12-26 Monsanto Technology Llc Soybean variety 01058353
US9661823B1 (en) 2016-06-01 2017-05-30 Monsanto Technology Llc Soybean variety 01057424
US9848562B1 (en) 2016-06-01 2017-12-26 Monsanto Technology Llc Soybean variety 01058349
US10226002B2 (en) 2016-06-01 2019-03-12 Monsanto Technology Llc Soybean variety 01057408
US9832953B1 (en) 2016-06-01 2017-12-05 Monsanto Technology Llc Soybean variety 01057348
US9907248B2 (en) 2016-06-01 2018-03-06 Monsanto Technology Llc Soybean variety 01057390
US9974264B2 (en) 2016-06-01 2018-05-22 Monsanto Technology Llc Soybean variety 01057212
US9706738B1 (en) 2016-06-01 2017-07-18 Monsanto Technology Llc Soybean variety 01057282
US9788514B1 (en) 2016-06-01 2017-10-17 Monsanto Technology Llc Soybean variety 01057345
US9907250B2 (en) 2016-06-01 2018-03-06 Monsanto Technology Llc Soybean variety 01057493
US10231398B2 (en) 2016-06-01 2019-03-19 Monsanto Technology Llc Soybean variety 01057400
US9706739B1 (en) 2016-06-01 2017-07-18 Monsanto Technology Llc Soybean variety 01057290
US10104847B2 (en) 2016-06-01 2018-10-23 Monsanto Technology Llc Soybean variety 01057454
US9968054B2 (en) 2016-06-01 2018-05-15 Monsanto Technology Llc Soybean variety 01057207
US9867354B2 (en) 2016-06-02 2018-01-16 Monsanto Technology Llc Soybean variety 01058529
US9700010B1 (en) 2016-06-02 2017-07-11 Monsanto Technology Llc Soybean variety 01063871
US9848564B1 (en) 2016-06-02 2017-12-26 Monsanto Technology Llc Soybean variety 01058864
US9693529B1 (en) 2016-06-02 2017-07-04 Monsanto Technology Llc Soybean variety 01063878
US9693530B1 (en) 2016-06-02 2017-07-04 Monsanto Technology Llc Soybean variety 01063884
US9907255B2 (en) 2016-06-02 2018-03-06 Monsanto Technology Llc Soybean variety 01058767
US9700009B1 (en) 2016-06-02 2017-07-11 Monsanto Technology Llc Soybean variety 01058861
US9832954B1 (en) 2016-06-02 2017-12-05 Monsanto Technology Llc Soybean variety 01058711
US9907254B2 (en) 2016-06-02 2018-03-06 Monsanto Technology Llc Soybean variety 01058698
US9907256B2 (en) 2016-06-02 2018-03-06 Monsanto Technology Llc Soybean variety 01058822
US9832956B1 (en) 2016-06-02 2017-12-05 Monsanto Technology Llc Soybean variety 01063886
US9832955B1 (en) 2016-06-02 2017-12-05 Monsanto Technology Llc Soybean variety 01058718
US9901053B2 (en) 2016-06-02 2018-02-27 Monsanto Technology Llc Soybean variety 01058606
US9832957B1 (en) 2016-06-02 2017-12-05 Monsanto Technology Llc Soybean variety 01058863
US9872470B2 (en) 2016-06-02 2018-01-23 Monsanto Technology Llc Soybean variety 01058469
US9854773B2 (en) 2016-06-02 2018-01-02 Monsanto Technology Llc Soybean variety 01058444
US9907253B2 (en) 2016-06-02 2018-03-06 Monsanto Technology Llc Soybean variety 01058513
US9867353B2 (en) 2016-06-02 2018-01-16 Monsanto Technology Llc Soybean variety 01058528
US9807960B1 (en) 2016-06-02 2017-11-07 Monsanto Technology Llc Soybean variety 01063888
US9907260B2 (en) 2016-06-03 2018-03-06 Monsanto Technology Llc Soybean variety 01063906
US9730418B1 (en) 2016-06-03 2017-08-15 Monsanto Technology Llc Soybean variety 01063920
US9717213B1 (en) 2016-06-03 2017-08-01 Monsanto Technology Llc Soybean variety 01064120
US9907257B2 (en) 2016-06-03 2018-03-06 Monsanto Technology Llc Soybean variety 01063892
US9848565B1 (en) 2016-06-03 2017-12-26 Monsanto Technology Llc Soybean variety 01063939
US9730419B1 (en) 2016-06-03 2017-08-15 Monsanto Technology Llc Soybean variety 01064108
US9907258B2 (en) 2016-06-03 2018-03-06 Monsanto Technology Llc Soybean variety 01063898
US9907259B2 (en) 2016-06-03 2018-03-06 Monsanto Technology Llc Soybean variety 01063916
US9955645B2 (en) 2016-06-03 2018-05-01 Monsanto Technology Llc Soybean variety 01063902
US9717211B1 (en) 2016-06-03 2017-08-01 Monsanto Technology Llc Soybean variety 01063940
US9743625B1 (en) 2016-06-03 2017-08-29 Monsanto Technology Llc Soybean variety 01063903
US9901054B2 (en) 2016-06-03 2018-02-27 Monsanto Technology Llc Soybean variety 01063900
US9750221B1 (en) 2016-06-03 2017-09-05 Monsanto Technology Llc Soybean variety 01063935
US9832958B1 (en) 2016-06-03 2017-12-05 Monsanto Technology Llc Soybean variety 01064122
US9737037B1 (en) 2016-06-03 2017-08-22 Monsanto Technology Llc Soybean variety 01063921
US9907261B2 (en) 2016-06-03 2018-03-06 Monsanto Technology Llc Soybean variety 01063928
US9717212B1 (en) 2016-06-03 2017-08-01 Monsanto Technology Llc Soybean variety 01064113
US10219462B2 (en) 2016-06-03 2019-03-05 Monsanto Technology Llc Soybean variety 01063907
US10058047B2 (en) 2016-06-03 2018-08-28 Monsanto Technology Llc Soybean variety 01063918
US9730420B1 (en) 2016-06-06 2017-08-15 Monsanto Technology Llc Soybean variety 01064123
US9844197B1 (en) 2016-06-06 2017-12-19 Monsanto Technology Llc Soybean variety 01064151
US9832960B1 (en) 2016-06-06 2017-12-05 Monsanto Technology Llc Soybean variety 01064152
US9832961B1 (en) 2016-06-06 2017-12-05 Monsanto Technology Llc Soybean variety 01064167
US10064356B2 (en) 2016-06-06 2018-09-04 Monsanto Technology Llc Soybean variety 01064137
US9795106B1 (en) 2016-06-06 2017-10-24 Monsanto Technology Llc Soybean variety 01064150
US9867355B2 (en) 2016-06-06 2018-01-16 Monsanto Technology Llc Soybean variety 01064176
US9848566B1 (en) 2016-06-06 2017-12-26 Monsanto Technology Llc Soybean variety 01064124
US9832962B1 (en) 2016-06-06 2017-12-05 Monsanto Technology Llc Soybean variety 01064172
US9867356B2 (en) 2016-06-06 2018-01-16 Monsanto Technology Llc Soybean variety 01064143
US9848567B1 (en) 2016-06-06 2017-12-26 Monsanto Technology Llc Soybean variety 01064142
US9743627B1 (en) 2016-06-06 2017-08-29 Monsanto Technology Llc Soybean variety 01064182
US9832959B1 (en) 2016-06-06 2017-12-05 Monsanto Technology Llc Soybean variety 01064131
US9743626B1 (en) 2016-06-06 2017-08-29 Monsanto Technology Llc Soybean variety 01064148
US9839187B1 (en) 2016-06-06 2017-12-12 Monsanto Technology Llc Soybean variety 01064165
US9924673B2 (en) 2016-06-06 2018-03-27 Monsanto Technology Llc Soybean variety 01064169
US9872471B2 (en) 2016-06-06 2018-01-23 Monsanto Technology Llc Soybean variety 01064177
US9968055B2 (en) 2016-06-07 2018-05-15 Monsanto Technology Llc Soybean variety 01062064
US9974265B2 (en) 2016-06-07 2018-05-22 Monsanto Technology Llc Soybean variety 01057517
US9974266B2 (en) 2016-06-07 2018-05-22 Monsanto Technology Llc Soybean variety 01059566
US9848568B1 (en) 2016-06-07 2017-12-26 Monsanto Technology Llc Soybean variety 01064130
US9756824B1 (en) 2016-06-07 2017-09-12 Monsanto Technology Llc Soybean variety 01062058
US9924674B2 (en) 2016-06-07 2018-03-27 Monsanto Technology Llc Soybean variety 01057531
US9974267B2 (en) 2016-06-07 2018-05-22 Monsanto Technology Llc Soybean variety 01057526
US9743628B1 (en) 2016-06-07 2017-08-29 Monsanto Technology Llc Soybean variety 01062055
US9992945B2 (en) 2016-06-07 2018-06-12 Monsanto Technology Llc Soybean variety 01062057
US9999186B2 (en) 2016-06-07 2018-06-19 Monsanto Technology Llc Soybean variety 01059533
US9743629B1 (en) 2016-06-07 2017-08-29 Monsanto Technology Llc Soybean variety 01058714
US9844198B1 (en) 2016-06-07 2017-12-19 Monsanto Technology Llc Soybean variety 01063894
US9968056B2 (en) 2016-06-07 2018-05-15 Monsanto Technology Llc Soybean variety 01051641
CN106086010B (en) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 For detecting the nucleic acid sequence and its detection method of herbicide-tolerant soybean plant DBN9008
CN106086011B (en) * 2016-06-18 2019-10-18 北京大北农科技集团股份有限公司 For detecting the nucleic acid sequence and its detection method of herbicide-tolerant soybean plant DBN9004
CN106399302A (en) * 2016-06-24 2017-02-15 汕头出入境检验检疫局检验检疫技术中心 Duplex fluorescent PCR method for efficiently detecting specific genes of transgenic soybean MON87701 strain and transgenic soybean MON87708 strain simultaneously
WO2018064516A1 (en) * 2016-09-30 2018-04-05 Monsanto Technology Llc Method for selecting target sites for site-specific genome modification in plants
MA47081B1 (en) 2016-12-22 2021-04-30 Syngenta Participations Ag Polymorphs
GB201622007D0 (en) 2016-12-22 2017-02-08 And See Cambridge Display Tech Ltd Syngenta Participations Ag Polymorphs
UY37623A (en) 2017-03-03 2018-09-28 Syngenta Participations Ag DERIVATIVES OF OXADIAZOL THIOPHEN FUNGICIDES
US9913450B1 (en) 2017-03-29 2018-03-13 Monsanto Technology Llc Soybean cultivar 68071303
US9913449B1 (en) 2017-03-29 2018-03-13 Monsanto Technology Llc Soybean cultivar 67272133
US9961868B1 (en) 2017-03-29 2018-05-08 Monsanto Technology Llc Soybean cultivar 60111034
US9907281B1 (en) 2017-03-29 2018-03-06 Monsanto Technology Llc Soybean cultivar 64291310
US9930856B1 (en) 2017-03-29 2018-04-03 Monsanto Technology Llc Soybean cultivar 62030513
US9930858B1 (en) 2017-03-29 2018-04-03 Monsanto Technology Llc Soybean cultivar 62232543
US9930857B1 (en) 2017-03-29 2018-04-03 Monsato Technology Llc Soybean cultivar 61311131
US9913448B1 (en) 2017-03-29 2018-03-13 Monsanto Technology Llc Soybean cultivar 63281310
US9907280B1 (en) 2017-03-29 2018-03-06 Monsanto Technology Llc Soybean cultivar 64492916
US9999193B1 (en) 2017-03-29 2018-06-19 Monsanto Technology Llc Soybean cultivar 65090203
US10039249B1 (en) 2017-03-29 2018-08-07 Monsanto Technology Llc Soybean cultivar 61162227
US9907282B1 (en) 2017-03-29 2018-03-06 Monsanto Technology Llc Soybean cultivar 60371632
US9907283B1 (en) 2017-03-29 2018-03-06 Monsanto Technology Llc Soybean cultivar 62103227
US9907279B1 (en) 2017-03-29 2018-03-06 Monsanto Technology Llc Soybean cultivar 63282422
US9888659B1 (en) 2017-03-29 2018-02-13 Monsanto Technology Llc Soybean cultivar 61040705
US9894869B1 (en) 2017-03-29 2018-02-20 Monsanto Technology Llc Soybean cultivar 64091728
KR102615161B1 (en) 2017-03-31 2023-12-15 신젠타 파티서페이션즈 아게 fungicidal composition
BR112019020134B1 (en) 2017-03-31 2023-05-09 Syngenta Participations Ag FUNGICIDAL COMPOSITIONS
CN110506040A (en) 2017-04-03 2019-11-26 先正达参股股份有限公司 Kill the oxadiazole derivatives of microorganism
WO2018184986A1 (en) 2017-04-05 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
BR112019020739B1 (en) 2017-04-05 2023-12-19 Syngenta Participations Ag COMPOUNDS DERIVED FROM OXADIAZOLE MICROBIOCIDES AND THEIR USE, AGROCHEMICAL COMPOSITION, METHOD TO CONTROL OR PREVENT INFESTATION OF USEFUL PLANTS BY PHYTOPATHOGENIC MICROORGANISMS
WO2018184987A1 (en) 2017-04-05 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
WO2018184988A1 (en) 2017-04-05 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
WO2018184982A1 (en) 2017-04-05 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
WO2018184984A1 (en) 2017-04-05 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
WO2018185211A1 (en) 2017-04-06 2018-10-11 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
CN110709395A (en) 2017-06-02 2020-01-17 先正达参股股份有限公司 Microbicidal oxadiazole derivatives
WO2018219773A1 (en) 2017-06-02 2018-12-06 Syngenta Participations Ag Fungicidal compositions
BR112019026331A2 (en) 2017-06-14 2020-07-21 Syngenta Participations Ag fungicidal compositions
US10342198B2 (en) 2017-06-26 2019-07-09 Monsanto Technology Llc Soybean variety 01064381
US10039255B1 (en) 2017-06-26 2018-08-07 Monsanto Technology Llc Soybean variety 01064252
US10034447B1 (en) 2017-06-26 2018-07-31 Monsanto Technology Llc Soybean variety 01064421
US10368518B2 (en) 2017-06-26 2019-08-06 Monsanto Technology Llc Soybean variety 01068117
US10034448B1 (en) 2017-06-26 2018-07-31 Monsanto Technology Llc Soybean variety 01064516
US10383304B2 (en) 2017-06-26 2019-08-20 Monsanto Technology Llc Soybean variety 01063944
US10314274B2 (en) 2017-06-26 2019-06-11 Monsanto Technology Llc Soybean variety 01068109
US10342199B2 (en) 2017-06-26 2019-07-09 Monsanto Technology Llc Soybean variety 01068883
US10455788B2 (en) 2017-06-26 2019-10-29 Monsanto Technology Llc Soybean variety 01068888
US10039254B1 (en) 2017-06-26 2018-08-07 Monsanto Technology Llc Soybean variety 01064117
US10342197B2 (en) 2017-06-26 2019-07-09 Monsanto Technology Llc Soybean variety 01064349
US10314273B2 (en) 2017-06-26 2019-06-11 Monsanto Technology Llc Soybean variety 01068110
US10405511B2 (en) 2017-06-26 2019-09-10 Monsanto Technology Llc Soybean variety 01064350
US10010046B1 (en) 2017-06-26 2018-07-03 Monsanto Technology Llc Soybean variety 01064401
US10053699B1 (en) 2017-06-28 2018-08-21 Monsanto Technology Llc Soybean variety 01068892
US10182539B1 (en) 2017-06-28 2019-01-22 Monsanto Technology Llc Soybean variety 5PDZB74
US10172310B1 (en) 2017-06-28 2019-01-08 Monsanto Technology Llc Soybean variety 5PYFS21
US10206349B1 (en) 2017-06-28 2019-02-19 Monsanto Technology Llc Soybean variety 5PPHU37
US10196646B2 (en) 2017-06-28 2019-02-05 Monsanto Technology Llc Soybean variety 01064344
US10244705B2 (en) 2017-06-28 2019-04-02 Monsanto Technology Llc Soybean variety 01064265
US10178838B1 (en) 2017-06-28 2019-01-15 Monsanto Technology Llc Soybean variety 5PQNG18
US10194612B2 (en) 2017-06-28 2019-02-05 Monsanto Technology Llc Soybean variety 01068093
BR112019027900A2 (en) 2017-06-28 2020-07-21 Syngenta Participations Ag fungicidal compositions
US10053700B1 (en) 2017-06-28 2018-08-21 Monsanto Technology Llc Soybean variety 01064288
US10244704B2 (en) 2017-06-28 2019-04-02 Monsanto Technologies Llc Soybean variety 01068084
US10368511B2 (en) 2017-06-28 2019-08-06 Monsanto Technology Llc Soybean variety 01068085
US10201138B2 (en) 2017-06-28 2019-02-12 Monsanto Technology Llc Soybean variety 01064519
US10011845B1 (en) 2017-06-28 2018-07-03 Monsanto Technology Llc Soybean variety 01068893
US10178837B1 (en) 2017-06-28 2019-01-15 Monsanto Technology Llc Soybean variety 5PHQP31
US10349598B2 (en) 2017-06-28 2019-07-16 Monsanto Technology Llc Soybean variety 01068088
US10188059B1 (en) 2017-06-28 2019-01-29 Monsanto Technology Llc Soybean variety 5PUWT62
US10206350B1 (en) 2017-06-28 2019-02-19 Monsanto Technology Llc Soybean variety 5PDSD70
US10036027B1 (en) 2017-06-28 2018-07-31 Monsanto Technology Llc Soybean variety 01064536
US10292358B2 (en) 2017-06-28 2019-05-21 Monsanto Technology Llc Soybean variety 01064327
US10194613B2 (en) 2017-06-28 2019-02-05 Monsanto Technology Llc Soybean variety 01064363
US10271493B2 (en) 2017-06-28 2019-04-30 Monsanto Technology Llc Soybean variety 01064280
US10368512B2 (en) 2017-06-28 2019-08-06 Monsanto Technology Llc Soybean variety 01068083
US10231403B2 (en) 2017-07-06 2019-03-19 Monsanto Technology Llc Soybean variety 01068141
US10064382B1 (en) 2017-07-06 2018-09-04 Monsanto Technology Llc Soybean variety 01064035
US10238057B2 (en) 2017-07-06 2019-03-26 Monsanto Technology Llc Soybean variety 01064461
US10201139B2 (en) 2017-07-06 2019-02-12 Monsanto Technology Llc Soybean variety 01068144
US10064367B1 (en) 2017-07-06 2018-09-04 Monsanto Technology Llc Soybean variety 01067515
US10244706B2 (en) 2017-07-06 2019-04-02 Monsanto Technology Llc Soybean variety 01064571
US10098305B1 (en) 2017-07-06 2018-10-16 Monsanto Technology Llc Soybean variety 01064403
US10070616B1 (en) 2017-07-06 2018-09-11 Monsanto Technology Llc Soybean variety 01067507
US10194616B2 (en) 2017-07-06 2019-02-05 Monsanto Technology Llc Soybean variety 01064568
US10194614B2 (en) 2017-07-06 2019-02-05 Monsanto Technology Llc Soybean variety 01064567
US10070617B1 (en) 2017-07-06 2018-09-11 Monsanto Technology Llc Soybean variety 01063975
US10064379B1 (en) 2017-07-06 2018-09-04 Monsanto Technology Llc Soybean variety 01064450
US10064381B1 (en) 2017-07-06 2018-09-04 Monsanto Technology Llc Soybean variety 01067510
WO2019011926A1 (en) 2017-07-11 2019-01-17 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
BR112020000456A2 (en) 2017-07-11 2020-07-21 Syngenta Participations Ag microbiocidal oxadiazole derivatives
WO2019011923A1 (en) 2017-07-11 2019-01-17 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
WO2019011928A1 (en) 2017-07-11 2019-01-17 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
WO2019012001A1 (en) 2017-07-12 2019-01-17 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
BR112020000371A2 (en) 2017-07-12 2020-07-14 Syngenta Participations Ag microbiocidal oxadiazole derivatives
WO2019012003A1 (en) 2017-07-13 2019-01-17 Syngenta Participations Ag Microbiocidal oxadiazole derivatives
US10064368B1 (en) 2017-07-19 2018-09-04 Monsanto Technology Llc Soybean variety 01068133
US10238076B2 (en) 2017-07-19 2019-03-26 Monsanto Technology, Llc Soybean variety 01068130
US10398105B2 (en) 2017-07-19 2019-09-03 Monsanto Technology Llc Soybean variety 01064198
US10194617B1 (en) 2017-07-19 2019-02-05 Monsanto Technology Llc Soybean variety 01068066
US10206351B2 (en) 2017-07-19 2019-02-19 Monsanto Technology Llc Soybean variety 01068060
US10212901B2 (en) 2017-07-19 2019-02-26 Monsanto Technology Llc Soybean variety 01068895
US10058060B1 (en) 2017-07-19 2018-08-28 Monsanto Technology Llc Soybean variety 01068082
US10098306B1 (en) 2017-07-19 2018-10-16 Monsanto Technology Llc Soybean variety 01068070
US10064369B1 (en) 2017-07-19 2018-09-04 Monsanto Technology Llc Soybean variety 01068131
US10231422B2 (en) 2017-07-19 2019-03-19 Monsanto Technology Llc Soybean variety 01068134
US10206352B2 (en) 2017-07-19 2019-02-19 Monsanto Technology Llc Soybean variety 01068065
US10231405B2 (en) 2017-07-19 2019-03-19 Monsanto Technology Llc Soybean variety 01068135
US10426116B2 (en) 2017-07-19 2019-10-01 Monsanto Technology Llc Soybean variety 01068124
US10231404B2 (en) 2017-07-19 2019-03-19 Monsanto Technology Llc Soybean variety 01064187
US10440908B2 (en) 2017-07-19 2019-10-15 Monsanto Technology Llc Soybean variety 01064245
US10356997B2 (en) 2017-07-19 2019-07-23 Monsanto Technology Llc Soybean variety 01068891
US10058061B1 (en) 2017-07-19 2018-08-28 Monsanto Technology Llc Soybean variety 01068112
US10219466B2 (en) 2017-07-19 2019-03-05 Monsanto Technology Llc Soybean variety 01064243
US10136602B1 (en) 2017-07-19 2018-11-27 Monsanto Technology Llc Soybean variety 01068087
US10231421B2 (en) 2017-07-19 2019-03-19 Monsanto Technology Llc Soybean variety 01064231
US10314275B2 (en) 2017-07-19 2019-06-11 Monsanto Technology Llc Soybean variety 01064238
US10098308B1 (en) 2017-07-20 2018-10-16 Monsanto Technology Llc Soybean variety 01068054
US10117401B1 (en) 2017-07-20 2018-11-06 Monsanto Technology Llc Soybean variety 01064488
US10231406B2 (en) 2017-07-20 2019-03-19 Monsanto Technology Llc Soybean variety 01068831
US10064370B1 (en) 2017-07-20 2018-09-04 Monsanto Technology Llc Soybean variety 01064806
US10117400B1 (en) 2017-07-20 2018-11-06 Monsanto Technology Llc Soybean variety 01064185
US10285367B2 (en) 2017-07-20 2019-05-14 Monsanto Technology Llc Soybean variety 01064508
US10098307B1 (en) 2017-07-20 2018-10-16 Monsanto Technology Llc Soybean variety 01064838
US10143167B1 (en) 2017-07-20 2018-12-04 Monsanto Technology Llc Soybean variety 01064871
US10206353B2 (en) 2017-07-20 2019-02-19 Monsanto Technology Llc Soybean variety 01064485
US10058062B1 (en) 2017-07-20 2018-08-28 Monsanto Technology Llc Soybean variety 01064840
US10051823B1 (en) 2017-07-20 2018-08-21 Monsanto Technology Llc Soybean variety 01064868
US10244723B2 (en) 2017-07-20 2019-04-02 Monsanto Technology Llc Soybean variety 01068058
US10368513B2 (en) 2017-07-31 2019-08-06 Monsanto Technology Llc Soybean variety 01064206
US10117402B1 (en) 2017-07-31 2018-11-06 Monsanto Technology Llc Soybean variety 01068884
US10257994B2 (en) 2017-08-23 2019-04-16 Monsanto Technology Llc Soybean variety 01068143
US10231408B1 (en) 2017-08-23 2019-03-19 Monsanto Technology Llc Soybean variety 01064013
US10285368B2 (en) 2017-08-23 2019-05-14 Monsanto Technology Llc Soybean variety 01064056
US10117404B1 (en) 2017-08-23 2018-11-06 Monsanto Technology Llc Soybean variety 01064613
US10238059B2 (en) 2017-08-23 2019-03-26 Monsanto Technology Llc Soybean variety 01064460
US10271494B2 (en) 2017-08-23 2019-04-30 Monsanto Technology Llc Soybean variety 01068122
US10264748B2 (en) 2017-08-23 2019-04-23 Monsanto Technology Llc Soybean variety 01064212
US10117403B1 (en) 2017-08-23 2018-11-06 Monsanto Technology Llc Soybean variety 01064099
US10244708B2 (en) 2017-08-23 2019-04-02 Monsanto Technology Llc Soybean variety 01064594
US10231407B1 (en) 2017-08-23 2019-03-19 Monsanto Technology Llc Soybean variety 01063959
US10251354B2 (en) 2017-08-23 2019-04-09 Monsanto Technology Llc Soybean variety 01064596
US10130058B1 (en) 2017-08-24 2018-11-20 Monsanto Technology Llc Soybean variety 01064723
US10136603B1 (en) 2017-08-24 2018-11-27 Monsanto Technology Llc Soybean variety 01067535
US10130059B1 (en) 2017-08-24 2018-11-20 Monsanto Technology Llc Soybean variety 01068801
US10342200B2 (en) 2017-08-24 2019-07-09 Monsanto Technology Llc Soybean variety 01067525
US10231423B1 (en) 2017-08-24 2019-03-19 Monsanto Technology Llc Soybean variety 01064404
US10051824B1 (en) 2017-08-24 2018-08-21 Monsanto Technology Llc Soybean variety 01064393
US10045507B1 (en) 2017-08-24 2018-08-14 Monsanto Technology Llc Soybean variety 01064406
US10051825B1 (en) 2017-08-24 2018-08-21 Monsanto Technology Llc Soybean variety 01064400
US10130060B1 (en) 2017-08-24 2018-11-20 Monsanto Technology Llc Soybean variety 01068804
US10045506B1 (en) 2017-08-24 2018-08-14 Monsanto Technology Llc Soybean variety 01064415
US10123501B1 (en) 2017-08-24 2018-11-13 Monsanto Technology Llc Soybean variety 01064722
US10314276B2 (en) 2017-08-24 2019-06-11 Monsanto Technology Llc Soybean variety 01064370
US10039256B1 (en) 2017-08-24 2018-08-07 Monsanto Technology Llc Soybean variety 01064446
US10433504B2 (en) 2017-08-29 2019-10-08 Monsanto Technology Llc Soybean variety 01064105
US10238077B2 (en) 2017-08-29 2019-03-26 Monsanto Technology Llc Soybean variety 01064104
US10271495B2 (en) 2017-08-29 2019-04-30 Monsanto Technology Llc Soybean variety 01064036
US10257995B2 (en) 2017-08-29 2019-04-16 Monsanto Technology Llc Soybean variety 01064083
US10159205B1 (en) 2017-08-29 2018-12-25 Monsanto Technology Llc Soybean variety 01064633
US10271496B2 (en) 2017-08-29 2019-04-30 Monsanto Technology Llc Soybean variety 01064102
US10271497B2 (en) 2017-08-29 2019-04-30 Monsanto Technology Llc Soybean variety 01064054
US10314277B2 (en) 2017-08-29 2019-06-11 Monsanto Technology Llc Soybean variety 01064636
US10244724B2 (en) 2017-08-29 2019-04-02 Monsanto Technology Llc Soybean variety 01064667
US10159206B1 (en) 2017-08-29 2018-12-25 Monsanto Technology Llc Soybean variety 01067519
US10231409B1 (en) 2017-08-29 2019-03-19 Monsanto Technology Llc Soybean variety 01064026
US10485197B2 (en) 2017-08-29 2019-11-26 Monsanto Technology Llc Soybean variety 01064625
US10342201B2 (en) 2017-08-29 2019-07-09 Monsanto Technology Llc Soybean variety 01067531
US10314265B2 (en) 2017-08-29 2019-06-11 Monsanto Technology Llc Soybean variety 01064669
US10051826B1 (en) 2017-08-30 2018-08-21 Monsanto Technology Llc Soybean variety 01064189
US10194618B1 (en) 2017-08-30 2019-02-05 Monsanto Technology Llc Soybean variety 01064184
US10188061B1 (en) 2017-08-30 2019-01-29 Monsanto Technology Llc Soybean variety 01064486
US10219484B1 (en) 2017-08-30 2019-03-05 Monsanto Technology Llc Soybean variety 01064735
US10357007B2 (en) 2017-08-30 2019-07-23 Monsanto Technology Llc Soybean variety 01064743
US10342202B2 (en) 2017-08-30 2019-07-09 Monsanto Technology Llc Soybean variety 01064775
US10051827B1 (en) 2017-08-30 2018-08-21 Monsanto Technology Llc Soybean variety 01064164
US10219485B1 (en) 2017-08-30 2019-03-05 Monsanto Technology Llc Soybean variety 01069661
US10212902B1 (en) 2017-08-30 2019-02-26 Monsanto Technology Llc Soybean variety 01067523
US10117407B1 (en) 2017-08-30 2018-11-06 Monsanto Technology Llc Soybean variety 01064490
US10117405B1 (en) 2017-08-30 2018-11-06 Monsanto Technology Llc Soybean variety 01064511
US10314278B2 (en) 2017-08-30 2019-06-11 Monsanto Technology Llc Soybean variety 01064635
US10117409B1 (en) 2017-08-30 2018-11-06 Monsanto Technology Llc Soybean variety 01064756
US10231426B1 (en) 2017-08-30 2019-03-19 Monsanto Technology Llc Soybean variety 01064748
US10231424B1 (en) 2017-08-30 2019-03-19 Monsanto Technology Llc Soybean variety 01067526
US10117406B1 (en) 2017-08-30 2018-11-06 Monsanto Technology Llc Soybean variety 01064166
US10219467B1 (en) 2017-08-30 2019-03-05 Monsanto Technology Llc Soybean variety 01064677
US10271498B2 (en) 2017-08-30 2019-04-30 Monsanto Technology Llc Soybean variety 01064676
US10278350B2 (en) 2017-08-30 2019-05-07 Monsanto Technology Llc Soybean variety 01064672
US10188072B1 (en) 2017-08-30 2019-01-29 Monsanto Technology Llc Soybean variety 01064694
US10064371B1 (en) 2017-08-30 2018-09-04 Monsanto Technology Llc Soybean variety 01064809
US10231425B1 (en) 2017-08-30 2019-03-19 Monsanto Technology Llc Soybean variety 01067534
US10117408B1 (en) 2017-08-30 2018-11-06 Monsanto Technology Llc Soybean variety 01064491
UY37912A (en) 2017-10-05 2019-05-31 Syngenta Participations Ag PICOLINAMIDE DERIVATIVES FUNGICIDES THAT CONTAIN HETEROARILO OR HETEROARILOXI TERMINAL GROUPS
UY37913A (en) 2017-10-05 2019-05-31 Syngenta Participations Ag PICOLINAMIDE DERIVATIVES FUNGICIDES THAT CARRY A QUATERNARY TERMINAL GROUP
US11291205B2 (en) 2017-11-15 2022-04-05 Syngenta Participations Ag Microbiocidal picolinamide derivatives
CA3151283C (en) 2018-02-02 2024-03-19 Monsanto Technology Llc Dna constructs conferring herbicide tolerance
CN112020503A (en) 2018-04-26 2020-12-01 先正达参股股份有限公司 Microbicidal oxadiazole derivatives
US10602703B1 (en) * 2018-05-15 2020-03-31 Pioneer Hi-Bred International, Inc. Soybean variety 5PDEC49
US10420312B1 (en) 2018-05-24 2019-09-24 Monsanto Technology Llc Soybean variety 5PKGL32
US10368520B1 (en) 2018-05-24 2019-08-06 Monsanto Technology Llc Soybean variety 5PCDJ10
US10420313B1 (en) 2018-05-24 2019-09-24 Monsanto Technology Llc Soybean variety 5PKVF24
US10368519B1 (en) 2018-05-24 2019-08-06 Monsanto Technology Llc Soybean variety 5PYGS18
US10412921B1 (en) 2018-05-24 2019-09-17 Monsanto Technology Llc Soybean variety 5PYUP46
US10480005B1 (en) 2018-05-24 2019-11-19 Monsanto Technology Llc Soybean variety 5PEKB42
US10477818B1 (en) 2018-05-24 2019-11-19 Monsanto Technology Llc Soybean variety 5PTJB40
BR112020026877A2 (en) 2018-06-29 2021-04-06 Syngenta Crop Protection Ag MICROBIOCIDAL OXADIAZOL DERIVATIVES
WO2020007658A1 (en) 2018-07-02 2020-01-09 Syngenta Crop Protection Ag 3-(2-thienyl)-5-(trifluoromethyl)-1,2,4-oxadiazole derivatives as agrochemical fungicides
US20210267204A1 (en) 2018-07-16 2021-09-02 Syngenta Crop Protection Ag Microbiocidal oxadiazole derivatives
CN109182369B (en) * 2018-08-03 2021-01-12 浙江大学 Multi-herbicide-resistant expression vector, transformant and application thereof
US10398122B1 (en) 2018-08-06 2019-09-03 Monsanto Technology Llc Soybean cultivar 77252418
US10238080B1 (en) 2018-08-06 2019-03-26 Monsanto Technology Llc Soybean cultivar 79072143
US10264760B1 (en) 2018-08-06 2019-04-23 Monsanto Technology Llc Soybean cultivar 79380729
US10238066B1 (en) 2018-08-06 2019-03-26 Monsanto Technology Llc Soybean cultivar 73301323
US10342207B1 (en) 2018-08-06 2019-07-09 Monsanto Technology Llc Soybean cultivar 68381333
US10349608B1 (en) 2018-08-06 2019-07-16 Monsanto Technology Llc Soybean cultivar 70520427
US10349607B1 (en) 2018-08-06 2019-07-16 Monsanto Technology Llc Soybean cultivar 78091047
US10342206B1 (en) 2018-08-06 2019-07-09 Monsanto Technology Llc Soybean cultivar 60390131
US10321654B1 (en) 2018-08-06 2019-06-18 Monsanto Technology Llc Soybean cultivar 76222418
US10238079B1 (en) 2018-08-06 2019-03-26 Monsanto Technology Llc Soybean cultivar 77301427
US10362758B1 (en) 2018-08-06 2019-07-30 Monsanto Technology Llc Soybean cultivar 64272401
US10368521B1 (en) 2018-08-06 2019-08-06 Monsanto Technology Llc Soybean cultivar 70490447
US10258014B1 (en) 2018-08-06 2019-04-16 Monsanto Technology Llc Soybean cultivar 76051047
US10517255B1 (en) 2018-08-06 2019-12-31 Monsanto Technology Llc Soybean cultivar 62391708
US10357010B1 (en) 2018-08-06 2019-07-23 Monsanto Technology Llc Soybean cultivar 68352516
US10342204B1 (en) 2018-08-06 2019-07-09 Monsanto Technology Llc Soybean cultivar 65382631
US10258013B1 (en) 2018-08-06 2019-04-16 Monsanto Technology Llc Soybean cultivar 64442216
US11206791B2 (en) 2018-08-22 2021-12-28 Monsanto Technology Llc Soybean variety 01072337
US10602704B2 (en) 2018-08-22 2020-03-31 Monsanto Technology Llc Soybean variety 01068313
US10897870B2 (en) 2018-08-22 2021-01-26 Monsanto Technology Llc Soybean variety 01072322
US11096363B2 (en) 2018-08-22 2021-08-24 Monsanto Technology Llc Soybean variety 01072331
US10905080B2 (en) 2018-08-22 2021-02-02 Monsanto Technology Llc Soybean variety 01072355
US11109552B2 (en) 2018-08-22 2021-09-07 Monsanto Technology Llc Soybean variety 01072332
US10582686B1 (en) 2018-08-22 2020-03-10 Monsanto Technology Llc Soybean variety 01064580
US10993404B2 (en) 2018-08-22 2021-05-04 Monsanto Technology Llc Soybean variety 01072356
US10555481B1 (en) 2018-08-22 2020-02-11 Monsanto Technology Llc Soybean variety 01068364
US10973199B2 (en) 2018-08-22 2021-04-13 Monsanto Technology Llc Soybean variety 01072341
US10624302B2 (en) 2018-08-22 2020-04-21 Monsanto Technology Llc Soybean variety 01072280
US10939652B2 (en) 2018-08-22 2021-03-09 Monsanto Technology Llc Soybean variety 01072350
US10905081B2 (en) 2018-08-22 2021-02-02 Monsanto Technology Llc Soybean variety 01072329
US10973198B2 (en) 2018-08-22 2021-04-13 Monsanto Technology Llc Soybean variety 01072340
US10602689B2 (en) 2018-08-22 2020-03-31 Monsanto Technology Llc Soybean variety 01068908
US10918065B2 (en) 2018-08-22 2021-02-16 Monsanto Technology Llc Soybean variety 01072343
US11026389B2 (en) 2018-08-22 2021-06-08 Monsanto Technology Llc Soybean variety 01072367
US10555482B1 (en) 2018-08-22 2020-02-11 Monsanto Technology Llc Soybean variety 01068427
US10624303B2 (en) 2018-08-22 2020-04-21 Monsanto Technology Llc Soybean variety 01072279
US10575489B1 (en) 2018-08-22 2020-03-03 Monsanto Technology Llc Soybean variety 01067545
US10561098B1 (en) 2018-08-22 2020-02-18 Monsanto Technology Llc Soybean variety 01067649
US10973201B2 (en) 2018-08-22 2021-04-13 Monsanto Technology Llc Soybean variety 01072339
US10624285B2 (en) 2018-08-22 2020-04-21 Monsanto Technology Llc Soybean variety 01068376
US10561099B1 (en) 2018-08-22 2020-02-18 Monsanto Technology Llc Soybean variety 01068352
US10897868B2 (en) 2018-08-22 2021-01-26 Monsanto Technology Llc Soybean variety 01072327
US10897869B2 (en) 2018-08-22 2021-01-26 Monsanto Technology Llc Soybean variety 01072333
US10555484B1 (en) 2018-08-22 2020-02-11 Monsanto Technology Llc Soybean variety 01068903
US11044876B2 (en) 2018-08-22 2021-06-29 Monsanto Technology Llc Soybean variety 01072345
US10555483B1 (en) 2018-08-22 2020-02-11 Monsanto Technology Llc Soybean variety 01067598
US11109553B2 (en) 2018-08-22 2021-09-07 Monsanto Technology Llc Soybean variety 01072328
US10999998B2 (en) 2018-08-22 2021-05-11 Monsanto Technology Llc Soybean variety 01072346
US10973200B2 (en) 2018-08-22 2021-04-13 Monsanto Technology Llc Soybean variety 01072342
US11013197B2 (en) 2018-08-22 2021-05-25 Monsanto Technology Llc Soybean variety 01072368
US10575490B1 (en) 2018-08-22 2020-03-03 Monsanto Technology Llc Soybean variety 01067547
US10555480B1 (en) 2018-08-22 2020-02-11 Monsanto Technology Llc Soybean variety 01068350
US10617089B2 (en) 2018-08-22 2020-04-14 Monsanto Technology Llc Soybean variety 01067827
US10524446B1 (en) 2018-08-23 2020-01-07 Monsanto Technology Llc Soybean variety 01072245
US10638707B2 (en) 2018-08-23 2020-05-05 Monsanto Technology Llc Soybean variety 01067639
US10499601B1 (en) 2018-08-23 2019-12-10 Monsanto Technology Llc Soybean variety 01068356
US10512239B1 (en) 2018-08-23 2019-12-24 Monsanto Technology Llc Soybean variety 01072273
US10631499B2 (en) 2018-08-23 2020-04-28 Monsanto Technology Llc Soybean variety 01072291
US10506786B1 (en) 2018-08-23 2019-12-17 Monsanto Technology Llc Soybean variety 01067789
US10555486B1 (en) 2018-08-23 2020-02-11 Monsanto Technology Llc Soybean variety 01068910
US10492450B1 (en) 2018-08-23 2019-12-03 Monsanto Technology Llc Soybean variety 01064752
US10548284B1 (en) 2018-08-23 2020-02-04 Monsanto Technology Llc Soybean variety 01072294
US10463000B1 (en) 2018-08-23 2019-11-05 Monsanto Technology Llc Soybean variety 01072224
US10448609B1 (en) 2018-08-23 2019-10-22 Monsanto Technology Llc Soybean variety 01072293
US10555485B1 (en) 2018-08-23 2020-02-11 Monsanto Technology Llc Soybean variety 01073532
US10448608B1 (en) 2018-08-23 2019-10-22 Monsanto Technology Llc Soybean variety 01067812
US10631486B2 (en) 2018-08-23 2020-04-28 Monsanto Technology Llc Soybean variety 01068187
US10609888B2 (en) 2018-08-23 2020-04-07 Monsanto Technology Llc Soybean variety 01068346
US10687501B2 (en) 2018-08-23 2020-06-23 Monsanto Technology Llc Soybean variety 01068438
US10687502B2 (en) 2018-08-23 2020-06-23 Monsanto Technology Llc Soybean variety 01068433
US10638708B2 (en) 2018-08-23 2020-05-05 Monsanto Technology Llc Soybean variety 01068325
US10624287B2 (en) 2018-08-23 2020-04-21 Monsanto Technology Llc Soybean variety 01072275
US10624286B2 (en) 2018-08-23 2020-04-21 Monsanto Technology Llc Soybean variety 01072276
US10561106B1 (en) 2018-08-24 2020-02-18 Monsanto Technology Llc Soybean variety 01068466
US10631506B2 (en) 2018-08-24 2020-04-28 Monsanto Technology Llc Soybean variety 01068838
US10531629B1 (en) 2018-08-24 2020-01-14 Monsanto Technology Llc Soybean variety 01072223
US10660292B2 (en) 2018-08-24 2020-05-26 Monsanto Technology Llc Soybean variety 01068157
US10582690B1 (en) 2018-08-24 2020-03-10 Monsanto Technology Llc Soybean variety 01067790
US10582688B1 (en) 2018-08-24 2020-03-10 Monsanto Technology Llc Soybean variety 01067543
US10448610B1 (en) 2018-08-24 2019-10-22 Monsanto Technology Llc Soybean variety 01067781
US10561105B1 (en) 2018-08-24 2020-02-18 Monsanto Technology Llc Soybean variety 01072235
US10561100B1 (en) 2018-08-24 2020-02-18 Monsanto Technology Llc Soybean variety 01067742
US10506787B1 (en) 2018-08-24 2019-12-17 Monsanto Technology Llc Soybean variety 01072220
US10555487B1 (en) 2018-08-24 2020-02-11 Monsanto Technology Llc Soybean variety 01067694
US10542721B1 (en) 2018-08-24 2020-01-28 Monsanto Technology Llc Soybean variety 01072231
US10561102B1 (en) 2018-08-24 2020-02-18 Monsanto Technology Llc Soybean variety 01068182
US10582689B1 (en) 2018-08-24 2020-03-10 Monsanto Technology Llc Soybean variety 01067573
US10485213B1 (en) 2018-08-24 2019-11-26 Monsanto Technology Llc Soybean variety 01068968
US10517259B1 (en) 2018-08-24 2019-12-31 Monsanto Technology Llc Soybean variety 01068824
US10561103B1 (en) 2018-08-24 2020-02-18 Monsanto Technology Llc Soybean variety 01073501
US10617090B2 (en) 2018-08-24 2020-04-14 Monsanto Technology Llc Soybean variety 01068163
US10555488B1 (en) 2018-08-24 2020-02-11 Monsanto Technology Llc Soybean variety 01067714
US10477821B1 (en) 2018-08-24 2019-11-19 Monsanto Technology Llc Soybean variety 01067769
US10582687B1 (en) 2018-08-24 2020-03-10 Monsanto Technology Llc Soybean variety 01068359
US10548285B1 (en) 2018-08-24 2020-02-04 Monsanto Technology Llc Soybean variety 01067703
US10512241B1 (en) 2018-08-24 2019-12-24 Monsanto Technology Llc Soybean variety 01072232
US10631504B2 (en) 2018-08-24 2020-04-28 Monsanto Technology Llc Soybean variety 01072295
US10624304B2 (en) 2018-08-24 2020-04-21 Monsanto Technology Llc Soybean variety 01068381
US10631503B2 (en) 2018-08-24 2020-04-28 Monsanto Technology Llc Soybean variety 01067814
US10645898B2 (en) 2018-08-24 2020-05-12 Monsanto Technology Llc Soybean variety 01068192
US10631505B2 (en) 2018-08-24 2020-04-28 Monsanto Technology Llc Soybean variety 01069676
US10602705B2 (en) 2018-08-24 2020-03-31 Monsanto Technology Llc Soybean variety 01068821
US10631500B2 (en) 2018-08-24 2020-04-28 Monsanto Technology Llc Soybean variety 01067788
US10631501B2 (en) 2018-08-24 2020-04-28 Monsanto Technology Llc Soybean variety 01067793
US10470425B1 (en) 2018-08-24 2019-11-12 Monsanto Technology Llc Soybean variety 01067783
US10631507B2 (en) 2018-08-24 2020-04-28 Monsanto Technology Llc Soybean variety 01068485
US10470395B1 (en) 2018-08-24 2019-11-12 Monsanto Technology Llc Soybean variety 01067663
US10638709B2 (en) 2018-08-24 2020-05-05 Monsanto Technology Llc Soybean variety 01068191
US10492451B1 (en) 2018-08-24 2019-12-03 Monsanto Technology Llc Soybean variety 0107226
US10512240B1 (en) 2018-08-24 2019-12-24 Monsanto Technology Llc Soybean variety 01072228
US10561101B1 (en) 2018-08-24 2020-02-18 Monsanto Technology Llc Soybean variety 01068184
US10638710B2 (en) 2018-08-24 2020-05-05 Monsanto Technology Llc Soybean variety 01068195
US10631502B2 (en) 2018-08-24 2020-04-28 Monsanto Technology Llc Soybean variety 01067840
US10561104B1 (en) 2018-08-24 2020-02-18 Monsanto Technology Llc Soybean variety 01072234
US10575491B1 (en) 2018-08-24 2020-03-03 Monsanto Technology Llc Soybean variety 01072289
US10463010B1 (en) 2018-08-24 2019-11-05 Monsanto Technology Llc Soybean variety 01068189
US10602691B2 (en) 2018-08-27 2020-03-31 Monsanto Technology Llc Soybean variety 01072253
US10687503B2 (en) 2018-08-27 2020-06-23 Monsanto Technology Llc Soybean variety 01068213
US10582678B1 (en) 2018-08-27 2020-03-10 Monsanto Technology Llc Soybean variety 01072239
US10463011B1 (en) 2018-08-27 2019-11-05 Monsanto Technology Llc Soybean variety 01068162
US10582679B1 (en) 2018-08-27 2020-03-10 Monsanto Technology Llc Soybean variety 01072243
US10561107B1 (en) 2018-08-27 2020-02-18 Monsanto Technology Llc Soybean variety 01068807
US10575493B1 (en) 2018-08-27 2020-03-03 Monsanto Technology Llc Soybean variety 01067819
US10512242B1 (en) 2018-08-27 2019-12-24 Monsanto Technology Llc Soybean variety 01077436
US10602706B2 (en) 2018-08-27 2020-03-31 Monsanto Technology Llc Soybean variety 01077437
US10575492B1 (en) 2018-08-27 2020-03-03 Monsanto Technology Llc Soybean variety 01068915
US10499602B1 (en) 2018-08-27 2019-12-10 Monsanto Technology Llc Soybean variety 01067553
US10743491B2 (en) 2018-08-27 2020-08-18 Monsanto Technology Llc Soybean variety 01077432
US10568290B1 (en) 2018-08-27 2020-02-25 Monsanto Technology Llc Soybean variety 01068219
US10477822B1 (en) 2018-08-27 2019-11-19 Monsanto Technology Llc Soybean variety 01069686
US10506788B1 (en) 2018-08-27 2019-12-17 Monsanto Technology Llc Soybean variety 01072292
US10548286B1 (en) 2018-08-27 2020-02-04 Monsanto Technology Llc Soybean variety 01068171
US10492452B1 (en) 2018-08-27 2019-12-03 Monsanto Technology Llc Soybean variety 01067646
US10555489B1 (en) 2018-08-27 2020-02-11 Monsanto Technology Llc Soybean variety 01068972
US10743492B2 (en) 2018-08-27 2020-08-18 Monsanto Technology Llc Soybean variety 01077433
US10499603B1 (en) 2018-08-27 2019-12-10 Monsanto Technology Llc Soybean variety 01068207
US10631508B2 (en) 2018-08-27 2020-04-28 Monsanto Technology Llc Soybean variety 01068228
US10463012B1 (en) 2018-08-27 2019-11-05 Monsanto Technology Llc Soybean variety 01069688
US10660294B2 (en) 2018-08-27 2020-05-26 Monsanto Technology Llc Soybean variety 01077435
US10477823B1 (en) 2018-08-27 2019-11-19 Monsanto Technology Llc Soybean variety 01069720
US10660293B2 (en) 2018-08-27 2020-05-26 Monsanto Technology Llc Soybean variety 01077434
US10492453B1 (en) 2018-08-27 2019-12-03 Monsanto Technology Llc Soybean variety 01072254
US10492454B1 (en) 2018-08-27 2019-12-03 Monsanto Technology Llc Soybean variety 01072256
US10602692B2 (en) 2018-08-27 2020-03-31 Monsanto Technology Llc Soybean variety 01072252
US10463013B1 (en) 2018-08-27 2019-11-05 Monsanto Technology Llc Soybean variety 01069709
US10631509B2 (en) 2018-08-27 2020-04-28 Monsanto Technology Llc Soybean variety 01067807
US10743493B2 (en) 2018-08-27 2020-08-18 Monsanto Technology Llc Soybean variety 01077438
US10602690B2 (en) 2018-08-27 2020-03-31 Monsanto Technology Llc Soybean variety 01068227
US10568291B1 (en) 2018-08-27 2020-02-25 Monsanto Technology Llc Soybean variety 01067741
CN113195462A (en) 2018-10-17 2021-07-30 先正达农作物保护股份公司 Microbicidal oxadiazole derivatives
AR116628A1 (en) 2018-10-18 2021-05-26 Syngenta Crop Protection Ag MICROBIOCIDAL COMPOUNDS
US10506789B1 (en) 2018-12-04 2019-12-17 Monsanto Technology Llc Soybean cultivar 61381330
WO2020165403A1 (en) 2019-02-15 2020-08-20 Syngenta Crop Protection Ag Phenyl substituted thiazole derivatives as microbiocidal compounds
CA3129347A1 (en) 2019-02-20 2020-08-27 Syngenta Crop Protection Ag Use of spiropidion
GB201903942D0 (en) 2019-03-22 2019-05-08 Syngenta Crop Protection Ag Microbiocidal compounds
WO2020208095A1 (en) 2019-04-10 2020-10-15 Syngenta Crop Protection Ag Microbiocidal picolinamide derivatives
CN113966171A (en) 2019-04-10 2022-01-21 先正达农作物保护股份公司 Fungicidal compositions
US10631513B1 (en) 2019-06-04 2020-04-28 Monsanto Technology Llc Soybean cultivar 87181312
US10667484B1 (en) 2019-06-04 2020-06-02 Monsanto Technology Llc Soybean cultivar 82322805
US10617080B1 (en) 2019-06-04 2020-04-14 Monsanto Technology Llc Soybean cultivar 87352527
US10602693B1 (en) 2019-06-04 2020-03-31 Monsanto Technology Llc Soybean cultivar 81302732
US10624305B1 (en) 2019-06-04 2020-04-21 Monsanto Technology Llc Soybean cultivar 85161422
US10617091B1 (en) 2019-06-04 2020-04-14 Monsanto Technology Llc Soybean cultivar 86331141
US10631512B1 (en) 2019-06-04 2020-04-28 Monsanto Technology Llc Soybean cultivar 85322536
US10638686B1 (en) 2019-06-05 2020-05-05 Monsanto Technology Llc Soybean cultivar 84161023
US10548287B1 (en) 2019-06-05 2020-02-04 Monsanto Technology Llc Soybean cultivar 86411138
US10602708B1 (en) 2019-06-05 2020-03-31 Monsanto Technology Llc Soybean cultivar 82370721
US10660295B1 (en) 2019-06-05 2020-05-26 Monsanto Technology Llc Soybean cultivar 86072029
US10624288B1 (en) 2019-06-05 2020-04-21 Monsanto Technology Llc Soybean cultivar 85061635
US10609883B1 (en) 2019-06-05 2020-04-07 Monsanto Technology Llc Soybean cultivar 82340103
US10638713B1 (en) 2019-06-05 2020-05-05 Monsanto Technology Llc Soybean cultivar 85220103
US10602707B1 (en) 2019-06-05 2020-03-31 Monsanto Technology Llc Soybean cultivar 85442619
US10602709B1 (en) 2019-06-05 2020-03-31 Monsanto Technology Llc Soybean cultivar 86360847
US10631514B1 (en) 2019-06-05 2020-04-28 Monsanto Technology Llc Soybean cultivar 80131402
US10638714B1 (en) 2019-06-06 2020-05-05 Monsanto Technology Llc Soybean cultivar 84062517
US10638687B1 (en) 2019-06-06 2020-05-05 Monsanto Technology Llc Soybean cultivar 88103087
US10561109B1 (en) 2019-06-06 2020-02-18 Monsanto Technology Llc Soybean cultivar 82042815
US10631487B1 (en) 2019-06-06 2020-04-28 Monsanto Technology Llc Soybean cultivar 83240828
US10813335B1 (en) 2019-06-06 2020-10-27 Monsanto Technology Llc Soybean cultivar 80261309
US10785950B1 (en) 2019-06-06 2020-09-29 Stine Seed Farm, Inc. Soybean cultivar 86190345
US10568284B1 (en) 2019-06-06 2020-02-25 Monsanto Technology Llc Soybean cultivar 87161804
US10548273B1 (en) 2019-06-06 2020-02-04 Monsanto Technology Llc Soybean cultivar 87120510
US10561108B1 (en) 2019-06-06 2020-02-18 Monsanto Technology Llc Soybean cultivar 80431932
US10548288B1 (en) 2019-06-06 2020-02-04 Mansanto Technology Llc Soybean cultivar 80372800
US10568292B1 (en) 2019-06-06 2020-02-25 Monsanto Technology Llc Soybean cultivar 80181322
US10617092B1 (en) 2019-06-06 2020-04-14 Monsanto Technology Llc Soybean cultivar 80411617
US10609889B1 (en) 2019-06-06 2020-04-07 Monsanto Technology Llc Soybean cultivar 89001711
US10548289B1 (en) 2019-06-06 2020-02-04 Monsanto Technology Llc Soybean cultivar 82290447
US10555491B1 (en) 2019-06-07 2020-02-11 Monsanto Technology Llc Soybean cultivar 83072718
US10548274B1 (en) 2019-06-07 2020-02-04 Monsanto Technology Llc Soybean cultivar 83262718
US10568294B1 (en) 2019-06-07 2020-02-25 Monsanto Technology Llc Soybean cultivar 82440038
US10548290B1 (en) 2019-06-07 2020-02-04 Monsanto Technology Llc Soybean cultivar 89031941
US10561089B1 (en) 2019-06-07 2020-02-18 Monsanto Technology Llc Soybean cultivar 84060345
US10561090B1 (en) 2019-06-07 2020-02-18 Monsanto Technology Llc Soybean cultivar 86370345
US10602710B1 (en) 2019-06-07 2020-03-31 Monsanto Technology Llc Soybean cultivar 89282206
US10555492B1 (en) 2019-06-07 2020-02-11 Monsanto Technology Llc Soybean cultivar 81430038
US10660296B1 (en) 2019-06-07 2020-05-26 Monsanto Technology Llc Soybean cultivar 84241941
US10555490B1 (en) 2019-06-07 2020-02-11 Monsanto Technology Llc Soybean cultivar 85370909
US10568293B1 (en) 2019-06-07 2020-02-25 Monsanto Technology Llc Soybean cultivar AR 86112649
BR112021026861A2 (en) 2019-07-05 2022-02-22 Syngenta Crop Protection Ag Microbiocidal picolinamide derivatives
GB201910037D0 (en) 2019-07-12 2019-08-28 Syngenta Crop Protection Ag Microbiocidal compounds
US10993401B2 (en) 2019-07-29 2021-05-04 Monsanto Technology Llc Soybean variety 01073256
US10918050B1 (en) 2019-07-29 2021-02-16 Monsanto Technology Llc Soybean variety 01072791
US10912270B1 (en) 2019-07-29 2021-02-09 Monsanto Technology Llc Soybean variety 01072737
US10849300B1 (en) 2019-07-29 2020-12-01 Monsanto Technology Llc Soybean variety 01073359
US10918051B1 (en) 2019-07-29 2021-02-16 Monsanto Technology Llc Soybean variety 01072804
US10925235B1 (en) 2019-07-29 2021-02-23 Monsanto Technology Llc Soybean variety 01072810
US10905072B1 (en) 2019-07-29 2021-02-02 Monsanto Technology Llc Soybean variety 01073330
US10932428B2 (en) 2019-07-29 2021-03-02 Monsanto Technology Llc Soybean variety 01072749
US10932429B2 (en) 2019-07-29 2021-03-02 Monsanto Technology Llc Soybean variety 01073265
US10918053B1 (en) 2019-07-29 2021-02-16 Monsanto Technology Llc Soybean variety 01073354
US10918052B1 (en) 2019-07-29 2021-02-16 Monsanto Technology Llc Soybean variety 01073365
US10939643B2 (en) 2019-07-29 2021-03-09 Monsanto Technology Llc Soybean variety 01072768
US10905071B1 (en) 2019-07-29 2021-02-02 Monsanto Technology Llc Soybean variety 01072744
US11096357B2 (en) 2019-07-29 2021-08-24 Monsanto Technology Llc Soybean variety 01073351
US10918054B1 (en) 2019-07-29 2021-02-16 Monsanto Technology Llc Soybean variety 01073353
US10925238B1 (en) 2019-07-29 2021-02-23 Monsanto Technology Llc Soybean variety 01073320
US10869452B1 (en) 2019-07-29 2020-12-22 Monsanto Technology Llc Soybean variety 01072767
US10925237B1 (en) 2019-07-29 2021-02-23 Monsanto Technology Llc Soybean variety 01073288
US10863708B1 (en) 2019-07-29 2020-12-15 Monsanto Technology Llc Soybean variety 01073439
US10863709B1 (en) 2019-07-29 2020-12-15 Monsanto Technology Llc Soybean variety 01072789
US10925236B1 (en) 2019-07-29 2021-02-23 Monsanto Technology Llc Soybean variety 01072823
US10888063B1 (en) 2019-07-30 2021-01-12 Monsanto Technology Llc Soybean variety 01072800
US10918055B1 (en) 2019-07-30 2021-02-16 Monsanto Technology Llc Soybean variety 01073472
US10881073B1 (en) 2019-07-30 2021-01-05 Monsanto Technology Llc Soybean variety 01072782
US10827714B1 (en) 2019-07-30 2020-11-10 Monsanto Technology Llc Soybean variety 01073443
US10925239B1 (en) 2019-07-30 2021-02-23 Monsanto Technology Llc Soybean variety 01073452
US10856507B1 (en) 2019-07-30 2020-12-08 Monsanto Technology Llc Soybean variety 01073444
US10925240B1 (en) 2019-07-30 2021-02-23 Monsanto Technology Llc Soybean variety 01073455
US10939645B2 (en) 2019-07-30 2021-03-09 Monsanto Technology Llc Soybean variety 01073480
US11266100B2 (en) 2019-07-30 2022-03-08 Monsanto Technology Llc Soybean variety 01073441
US10849301B1 (en) 2019-07-30 2020-12-01 Monsanto Technology Llc Soybean variety 01072784
US10939644B2 (en) 2019-07-30 2021-03-09 Monsanto Technology Llc Soybean variety 01073461
US10856508B1 (en) 2019-07-30 2020-12-08 Monsanto Technology Llc Soybean variety 01073445
US10897864B1 (en) 2019-07-30 2021-01-26 Monsanto Technology Llc Soybean variety 01073453
US10881074B1 (en) 2019-07-30 2021-01-05 Monsanto Technology Llc Soybean variety 01072802
US10939646B2 (en) 2019-07-30 2021-03-09 Monsanto Technology Llc Soybean variety 01073479
US10912271B1 (en) 2019-07-31 2021-02-09 Monsanto Technology Llc Soybean variety 01072822
US10827715B1 (en) 2019-07-31 2020-11-10 Monsanto Technology Llc Soybean variety 01077799
US10973194B2 (en) 2019-07-31 2021-04-13 Monsanto Technology Llc Soybean variety 01072867
US10918056B1 (en) 2019-07-31 2021-02-16 Monsanto Technology Llc Soybean variety 01077815
US10842116B1 (en) 2019-07-31 2020-11-24 Monsanto Technology Llc Soybean variety 01072832
US10939647B2 (en) 2019-07-31 2021-03-09 Monsanto Technology Llc Soybean variety 01072860
US10945397B2 (en) 2019-07-31 2021-03-16 Monsanto Technology Llc Soybean variety 01073506
US10869453B1 (en) 2019-07-31 2020-12-22 Monsanto Technology Llc Soybean variety 01072813
US11122759B2 (en) 2019-07-31 2021-09-21 Monsanto Technology Llc Soybean variety 01072870
US10874078B1 (en) 2019-07-31 2020-12-29 Monsanto Technology Llc Soybean variety 01072817
US10973193B2 (en) 2019-07-31 2021-04-13 Monsanto Technology Llc Soybean variety 01072864
US10939648B2 (en) 2019-07-31 2021-03-09 Monsanto Technology Llc Soybean variety 01072899
US10959389B2 (en) 2019-07-31 2021-03-30 Monsanto Technology Llc Soybean variety 01073503
US10842117B1 (en) 2019-07-31 2020-11-24 Monsanto Technology Llc Soybean variety 01072842
US10863710B1 (en) 2019-07-31 2020-12-15 Monsanto Technology Llc Soybean variety 01072819
US10905073B1 (en) 2019-08-01 2021-02-02 Monsanto Technology Llc Soybean variety 01072883
US11026387B2 (en) 2019-08-01 2021-06-08 Monsanto Technology Llc Soybean variety 01073016
US10905074B1 (en) 2019-08-01 2021-02-02 Monsanto Technology Llc Soybean variety 01072906
US10925241B1 (en) 2019-08-01 2021-02-23 Monsanto Technology Llc Soybean variety 01072974
US11026388B2 (en) 2019-08-01 2021-06-08 Monsanto Technology Llc Soybean variety 01073060
US11019788B2 (en) 2019-08-01 2021-06-01 Monsanto Technology Llc Soybean variety 01072980
US10905075B1 (en) 2019-08-01 2021-02-02 Monsanto Technology Llc Soybean variety 01073059
US11019789B2 (en) 2019-08-01 2021-06-01 Monsanto Technology Llc Soybean variety 01073534
US11006602B2 (en) 2019-08-01 2021-05-18 Monsanto Technology Llc Soybean variety 01072946
US11129346B2 (en) 2019-08-01 2021-09-28 Monsanto Technology Llc Soybean variety 01073533
US11019787B2 (en) 2019-08-01 2021-06-01 Monsanto Technology Llc Soybean variety 01072951
US11096359B2 (en) 2019-08-07 2021-08-24 Monsanto Technology Llc Soybean variety 01077377
US11019790B2 (en) * 2019-08-07 2021-06-01 Monsanto Technology Llc Soybean variety 01073304
US10980203B2 (en) 2019-08-07 2021-04-20 Monsanto Technology Llc Soybean variety 01073305
US10973195B2 (en) 2019-08-07 2021-04-13 Monsanto Technology Llc Soybean variety 01073325
US11076552B2 (en) 2019-08-07 2021-08-03 Monsanto Technology Llc Soybean variety 01073356
US10912273B1 (en) 2019-08-07 2021-02-09 Monsanto Technology Llc Soybean variety 01073355
US11109550B2 (en) 2019-08-15 2021-09-07 Monsanto Technology Llc Soybean variety 01073116
US11013196B2 (en) 2019-08-15 2021-05-25 Monsanto Technology Llc Soybean variety 01073094
US10952392B2 (en) 2019-08-15 2021-03-23 Monsanto Technology Llc Soybean variety 01073151
US10952393B2 (en) 2019-08-15 2021-03-23 Monsanto Technology Llc Soybean variety 01073169
US11044867B2 (en) 2019-08-15 2021-06-29 Monsanto Technology Llc Soybean variety 01073133
US11044866B2 (en) 2019-08-15 2021-06-29 Monsanto Technology Llc Soybean variety 01073114
US10952391B2 (en) 2019-08-15 2021-03-23 Monsanto Technology Llc Soybean variety 01073150
US11096361B2 (en) 2019-08-15 2021-08-24 Monsanto Technology Llc Soybean variety 01073072
US11096360B2 (en) 2019-08-15 2021-08-24 Monsanto Technology Llc Soybean variety 01067560
US10959390B2 (en) 2019-08-15 2021-03-30 Monsanto Technology Llc Soybean variety 01073069
US11044865B2 (en) 2019-08-15 2021-06-29 Monsanto Technology Llc Soybean variety 01073029
US11071271B2 (en) 2019-08-15 2021-07-27 Monsanto Technology Llc Soybean variety 01073065
US10945398B2 (en) 2019-08-15 2021-03-16 Monsanto Technology Llc Soybean variety 01073160
US11129347B2 (en) 2019-08-16 2021-09-28 Monsanto Technology Llc Soybean variety 01073210
US10905079B1 (en) 2019-08-16 2021-02-02 Monsanto Technology Llc Soybean variety 01073335
US11071272B2 (en) 2019-08-16 2021-07-27 Monsanto Technology Llc Soybean variety 01073195
US11160237B2 (en) 2019-08-16 2021-11-02 Monsanto Technology Llc Soybean variety 01073183
US10863712B1 (en) 2019-08-16 2020-12-15 Monsanto Technology Llc Soybean variety 01073281
US11044869B2 (en) 2019-08-16 2021-06-29 Monsanto Technology Llc Soybean variety 01073270
US10973196B2 (en) 2019-08-16 2021-04-13 Monsanto Technology Llc Soybean variety 01073237
US10869454B1 (en) 2019-08-16 2020-12-22 Monsanto Technology Llc Soybean variety 01073329
US11044868B2 (en) 2019-08-16 2021-06-29 Monsanto Technology Llc Soybean variety 01073261
US10856509B1 (en) 2019-08-16 2020-12-08 Monsanto Technology Llc Soybean variety 01073199
US11039591B2 (en) 2019-08-16 2021-06-22 Monsanto Technology Llc Soybean variety 01073340
US11039590B2 (en) 2019-08-16 2021-06-22 Monsanto Technology Llc Soybean variety 01073272
US11129349B2 (en) 2019-08-16 2021-09-28 Monsanto Technology Llc Soybean variety 01073350
US11006603B2 (en) 2019-08-16 2021-05-18 Monsanto Technology Llc Soybean variety 01072287
US10863711B1 (en) 2019-08-16 2020-12-15 Monsanto Technology Llc Soybean variety 01073279
US11109551B2 (en) 2019-08-16 2021-09-07 Monsanto Technology Llc Soybean variety 01073253
US10932430B1 (en) 2019-08-16 2021-03-02 Monsanto Technology Llc Soybean variety 01073203
US11096362B2 (en) 2019-08-16 2021-08-24 Monsanto Technology Llc Soybean variety 01073204
US11129348B2 (en) 2019-08-16 2021-09-28 Monsanto Technology Llc Soybean variety 01073219
US11185044B2 (en) 2019-09-16 2021-11-30 Monsanto Technology Llc Soybean variety 01077343
US11266110B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01077337
US11266106B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01081407
US11259494B2 (en) 2019-09-16 2022-03-01 Monsanto Technology Llc Soybean variety 01077345
US11185043B2 (en) 2019-09-16 2021-11-30 Monsanto Technology Llc Soybean variety 01077334
US11266109B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01077385
US11266105B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01077338
US11350595B2 (en) 2019-09-16 2022-06-07 Monsanto Technology, Llc Soybean variety 01077372
US11178841B2 (en) 2019-09-16 2021-11-23 Monsanto Technology Llc Soybean variety 01077386
US11284587B2 (en) 2019-09-16 2022-03-29 Monsanto Technology Llc Soybean variety 01077381
US11337400B2 (en) 2019-09-16 2022-05-24 Monsanto Technology, Llc Soybean variety 01077373
US11259493B2 (en) 2019-09-16 2022-03-01 Monsanto Technology Llc Soybean variety 01077341
US11266107B2 (en) 2019-09-16 2022-03-08 Monsanto Technology Llc Soybean variety 01081408
US11229182B2 (en) 2019-09-16 2022-01-25 Monsanto Technology Llc Soybean variety 01077387
US11576340B2 (en) 2019-09-17 2023-02-14 Monsanto Technology, Llc Soybean variety 01081414
US11297792B2 (en) 2019-09-17 2022-04-12 Monsanto Technology Llc Soybean variety 01077344
US11266111B2 (en) 2019-09-17 2022-03-08 Monsanto Technology Llc Soybean variety 01077383
US11576341B2 (en) * 2019-09-17 2023-02-14 Monsanto Technology, Llc Soybean variety 01072361
US11266108B2 (en) 2019-09-17 2022-03-08 Monsanto Technology Llc Soybean variety 01081409
US11330783B2 (en) 2019-10-18 2022-05-17 Monsanto Technology, Llc Soybean variety 01077336
US11330777B2 (en) 2019-10-18 2022-05-17 Monsanto Technology, Llc Soybean variety 01077390
US11252922B2 (en) 2019-10-18 2022-02-22 Monsanto Technology Llc Soybean variety 01077391
US11272679B2 (en) 2019-10-18 2022-03-15 Monsanto Technology Llc Soybean variety 01077330
US11252921B2 (en) 2019-10-18 2022-02-22 Monsanto Technology Llc Soybean variety 01077393
US11324181B2 (en) 2019-10-18 2022-05-10 Monsanto Technology Llc Soybean variety 01077335
US20230131427A1 (en) 2020-03-05 2023-04-27 Syngenta Crop Protection Ag Fungicidal compositions
UY39115A (en) 2020-03-05 2021-10-29 Syngenta Crop Protection Ag FUNGICIDE MIXTURES OF ARYL METHOXYACRYLATE DERIVATIVES
GB202006386D0 (en) 2020-04-30 2020-06-17 Syngenta Crop Protection Ag Microbiocidal Compounds
GB202006399D0 (en) 2020-04-30 2020-06-17 Syngenta Crop Protection Ag Microbiocidal compounds
GB202006480D0 (en) 2020-05-01 2020-06-17 Syngenta Crop Protection Ag Microbiocidal compounds
GB202006606D0 (en) 2020-05-05 2020-06-17 Syngenta Crop Protection Ag Microbiocidal compounds
CA3188279A1 (en) * 2020-07-31 2022-02-03 Inari Agriculture Technology, Inc. Inht26 transgenic soybean
US20240011043A1 (en) 2020-07-31 2024-01-11 Inari Agriculture Technology, Inc. Generation of plants with improved transgenic loci by genome editing
US11234406B1 (en) 2020-09-09 2022-02-01 Monsanto Technology Llc Soybean cultivar 98010818
US11234405B1 (en) 2020-09-09 2022-02-01 Monsanto Technology Llc Soybean cultivar 90110208
US11206797B1 (en) 2020-09-09 2021-12-28 Monsanto Technology Llc Soybean cultivar 97320218
US11252923B1 (en) 2020-09-09 2022-02-22 Monsanto Technology Llc Soybean cultivar 99003018
US11259495B1 (en) 2020-09-09 2022-03-01 Monsanto Technology Llc Soybean cultivar 90381408
US11330784B2 (en) 2020-09-11 2022-05-17 Monsanto Technology, Llc Soybean cultivar 95322700
US20220079073A1 (en) * 2020-09-11 2022-03-17 Monsanto Technology Llc Soybean cultivar 92111703
US11284592B1 (en) 2020-09-11 2022-03-29 Monsanto Technology Llc Soybean cultivar 91030603
US11357200B2 (en) 2020-09-11 2022-06-14 Monsanto Technology, Llc Soybean cultivar 92131600
US11317588B2 (en) 2020-09-11 2022-05-03 Stine Seed Farm, Inc. Soybean cultivar 97080600
US11439111B2 (en) 2020-09-11 2022-09-13 Monsanto Technology Llc Soybean cultivar 99354100
US11229183B1 (en) 2020-09-11 2022-01-25 Monsanto Technology Llc Soybean cultivar 93070703
US11350596B2 (en) 2020-09-11 2022-06-07 Monsanto Technology, Llc Soybean cultivar 96050600
US11310988B2 (en) 2020-09-11 2022-04-26 Monsanto Technology Llc Soybean cultivar 92340030
US11277996B1 (en) 2020-09-11 2022-03-22 Monsanto Technology Llc Soybean cultivar 97051600
US11317587B2 (en) 2020-09-11 2022-05-03 Stine Seed Farm, Inc. Soybean cultivar 96030600
US11406080B2 (en) 2020-09-15 2022-08-09 Stine Seed Farm, Inc. Soybean cultivar 92420140
US11412692B2 (en) 2020-09-15 2022-08-16 Monsanto Technology Llc Soybean cultivar 90290600
US11425879B2 (en) 2020-09-15 2022-08-30 Monsanto Technology, Llc Soybean cultivar 93054930
US11317589B2 (en) 2020-09-15 2022-05-03 Monsanto Technology Llc Soybean cultivar 95430820
US11363780B2 (en) 2020-09-15 2022-06-21 Monsanto Technology Llc Soybean cultivar 93272026
US11324182B2 (en) 2020-09-15 2022-05-10 Stine Seed Farm, Inc. Soybean cultivar 95060020
US11357201B2 (en) 2020-09-15 2022-06-14 Monsanto Technology, Llc Soybean cultivar 94411660
US11337401B2 (en) 2020-09-15 2022-05-24 Monsanto Technology, Llc Soybean cultivar 90370640
US11343989B2 (en) 2020-09-15 2022-05-31 Monsanto Technology, Llc Soybean cultivar 92350300
US11744211B2 (en) 2020-09-15 2023-09-05 Monsanto Technology, Llc Soybean cultivar 92310017
US11310989B2 (en) 2020-09-15 2022-04-26 Monsanto Technology Llc Soybean cultivar 94071660
US11606926B2 (en) 2020-09-15 2023-03-21 Monsanto Technology Llc Soybean cultivar 94030201
US11399497B2 (en) 2020-09-15 2022-08-02 Monsanto Technology, Llc Soybean cultivar 93333110
US11324183B2 (en) 2020-09-15 2022-05-10 Stine Seed Farm, Inc. Soybean cultivar 90250820
US11363778B2 (en) 2020-09-15 2022-06-21 Monsanto Technology Llc Soybean cultivar 90221620
US11419299B2 (en) 2020-09-15 2022-08-23 Monsanto Technology Llc Soybean cultivar 91361201
US11363779B2 (en) 2020-09-15 2022-06-21 Monsanto Technology Llc Soybean cultivar 90301201
US11343988B2 (en) 2020-09-15 2022-05-31 Stine Seed Farm, Inc. Soybean cultivar 91041800
US11343987B2 (en) 2020-09-15 2022-05-31 Stine Seed Farm, Inc. Soybean cultivar 90401320
US11324184B2 (en) 2020-09-15 2022-05-10 Stine Seed Farm, Inc. Soybean cultivar 97061850
US11399496B2 (en) 2020-09-15 2022-08-02 Monsanto Technology, Llc Soybean cultivar 90116100
US11350597B2 (en) 2020-09-15 2022-06-07 Monsanto Technology, Llc Soybean cultivar 94111580
US11369074B2 (en) 2020-09-15 2022-06-28 Monsanto Technology Llc Soybean cultivar 99122620
GB202014840D0 (en) 2020-09-21 2020-11-04 Syngenta Crop Protection Ag Microbiocidal compounds
US11510382B2 (en) 2020-11-02 2022-11-29 Monsanto Technology Llc Soybean variety 01078845
US11547075B2 (en) 2020-11-02 2023-01-10 Monsanto Technology Llc Soybean variety 01078822
US11477960B2 (en) 2020-11-02 2022-10-25 Monsanto Technology Llc Soybean variety 01077912
US11612128B2 (en) 2020-11-02 2023-03-28 Monsanto Technology Llc Soybean variety 01083666
US11653612B2 (en) 2020-11-02 2023-05-23 Monsanto Technology Llc Soybean variety 01077826
US11653613B2 (en) 2020-11-02 2023-05-23 Monsanto Technology Llc Soybean variety 01077829
US11596122B2 (en) 2020-11-02 2023-03-07 Monsanto Technology Llc Soybean variety 01077891
US11412694B2 (en) 2020-11-05 2022-08-16 Monsanto Technology Llc Soybean variety 01077905
US11350600B2 (en) 2020-11-05 2022-06-07 Monsanto Technology, Llc Soybean variety 01083699
US11284594B1 (en) 2020-11-05 2022-03-29 Monsanto Technology Llc Soybean variety 01077848
US11357205B2 (en) 2020-11-05 2022-06-14 Monsanto Technology Llc Soybean variety 01083631
US11343990B2 (en) 2020-11-05 2022-05-31 Monsanto Technology, Llc Soybean variety 01083439
US11343991B2 (en) 2020-11-05 2022-05-31 Monsanto Technology, Llc Soybean variety 01078115
US11445689B2 (en) 2020-11-05 2022-09-20 Monsanto Technology, Llc Soybean variety 01078100
US11445688B2 (en) 2020-11-05 2022-09-20 Monsanto Technology, Llc Soybean variety 01077870
US11284593B1 (en) 2020-11-05 2022-03-29 Monsanto Technology Llc Soybean variety 01083687
US11343992B2 (en) 2020-11-05 2022-05-31 Monsanto Technology, Llc Soybean variety 01078550
US11277997B1 (en) 2020-11-05 2022-03-22 Monsanto Technology Llc Soybean variety 01077853
US11457591B2 (en) 2020-11-05 2022-10-04 Monsanto Technology, Llc Soybean variety 01077893
US11343993B2 (en) 2020-11-05 2022-05-31 Monsanto Technology, Llc Soybean variety 01083650
US11533883B2 (en) 2020-11-05 2022-12-27 Monsanto Technology, Llc Soybean variety 01077890
US11357206B2 (en) 2020-11-06 2022-06-14 Monsanto Technology Llc Soybean variety 01077974
US11533884B2 (en) 2020-11-06 2022-12-27 Monsanto Technology, Llc Soybean variety 01083660
US11432518B2 (en) 2020-11-06 2022-09-06 Monsanto Technology, Llc Soybean variety 01078759
US11343996B1 (en) 2020-11-06 2022-05-31 Monsanto Technology, Llc Soybean variety 01077875
US11382301B2 (en) 2020-11-06 2022-07-12 Monsanto Technology, Llc Soybean variety 01077881
US11457601B2 (en) 2020-11-06 2022-10-04 Monsanto Technology Llc Soybean variety 01083638
US11369076B2 (en) 2020-11-06 2022-06-28 Monsanto Technology, Llc Soybean variety 01078749
US11350601B2 (en) 2020-11-06 2022-06-07 Monsanto Technology, Llc Soybean variety 01077876
US11510384B2 (en) 2020-11-06 2022-11-29 Monsanto Technology, Llc Soybean variety 01078675
US11547077B2 (en) 2020-11-06 2023-01-10 Monsanto Technology, Llc Soybean variety 01083688
US11445690B2 (en) 2020-11-06 2022-09-20 Monsanto Technology Llc Soybean variety 01083639
US11553671B2 (en) 2020-11-06 2023-01-17 Monsanto Technology, Llc Soybean variety 01083653
US11330785B1 (en) 2020-11-06 2022-05-17 Monsanto Technology, Llc Soybean variety 01077941
US11528879B2 (en) 2020-11-06 2022-12-20 Monsanto Technology, Llc Soybean variety 01083659
US11343995B1 (en) 2020-11-06 2022-05-31 Monsanto Technology, Llc Soybean variety 01083647
US11464195B2 (en) 2020-11-06 2022-10-11 Monsanto Technology Llc Soybean variety 01083654
US11484000B2 (en) 2020-11-06 2022-11-01 Monsanto Technology, Llc Soybean variety 01083661
US11477961B2 (en) 2020-11-06 2022-10-25 Monsanto Technology, Llc Soybean variety 01077940
US11343994B1 (en) 2020-11-06 2022-05-31 Monsanto Technology, Llc Soybean variety 01083655
US11547076B2 (en) 2020-11-06 2023-01-10 Monsanto Technology, Llc Soybean variety 01077931
US11457600B2 (en) 2020-11-06 2022-10-04 Monsanto Technology, Llc Soybean variety 01083646
US11439114B2 (en) 2020-11-06 2022-09-13 Monsanto Technology, Llc Soybean variety 01078032
TW202231187A (en) 2020-11-27 2022-08-16 瑞士商先正達農作物保護公司 Pesticidal compositions
UY39544A (en) 2020-12-02 2022-06-30 Syngenta Crop Protection Ag FUNGICIDE COMPOSITIONS COMPRISING A MIXTURE OF COMPONENTS (A) AND (B) AS ACTIVE PRINCIPLES
WO2022117650A1 (en) 2020-12-02 2022-06-09 Syngenta Crop Protection Ag Fungicidal compositions
AR125089A1 (en) 2021-03-19 2023-06-07 Syngenta Crop Protection Ag PESTICIDE COMPOSITIONS
WO2022233869A1 (en) 2021-05-04 2022-11-10 Syngenta Crop Protection Ag Use of clethodim for insect control
CN117597025A (en) 2021-07-02 2024-02-23 先正达农作物保护股份公司 Use of haloxyfop-R-methyl for insect control
US11666031B2 (en) 2021-08-24 2023-06-06 Monsanto Technology Llc Soybean cultivar 05140102
US11641837B2 (en) 2021-08-24 2023-05-09 Monsanto Technology Llc Soybean cultivar 02340134
US11696556B2 (en) 2021-08-24 2023-07-11 Monsanto Technology, Llc Soybean cultivar 07080746
US11647722B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 02140434
US11647724B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 03230124
US11647723B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 07060434
US11678635B2 (en) 2021-08-24 2023-06-20 Stine Seed Farm, Inc. Soybean cultivar 01160922
US11678636B2 (en) 2021-08-24 2023-06-20 Monsanto Technology, Llc Soybean cultivar 04070428
US11641838B2 (en) 2021-08-24 2023-05-09 Monsanto Technology Llc Soybean cultivar 04130514
US11647721B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 02330517
US11647720B2 (en) 2021-08-24 2023-05-16 Monsanto Technology Llc Soybean cultivar 01130157
US12029189B2 (en) 2021-08-30 2024-07-09 Monsanto Technology Llc Soybean cultivar 02170813
US11700827B2 (en) 2021-08-31 2023-07-18 Monsanto Technology, Llc Soybean cultivar 07220837
US11672229B2 (en) 2021-08-31 2023-06-13 Monsanto Technology, Llc Soybean cultivar 08180710
US11696558B2 (en) 2021-08-31 2023-07-11 Monsanto Technology, Llc Soybean cultivar 02021411
US11800847B2 (en) 2021-08-31 2023-10-31 Monsanto Technology, Llc Soybean cultivar 05050112
US11672230B2 (en) 2021-08-31 2023-06-13 Monsanto Technology, Llc Soybean cultivar 03260108
US11647725B2 (en) 2021-08-31 2023-05-16 Monsanto Technology Llc Soybean cultivar 07160416
US11647726B2 (en) 2021-08-31 2023-05-16 Monsanto Technology Llc Soybean cultivar 04070902
US11716947B2 (en) 2021-08-31 2023-08-08 Monsanto Technology, Llc Soybean cultivar 07030013
US11647727B2 (en) 2021-08-31 2023-05-16 Monsanto Technology Llc Soybean cultivar 04120446
US11700825B2 (en) 2021-08-31 2023-07-18 Monsanto Technology, Llc Soybean cultivar 06170324
US11856914B2 (en) 2021-08-31 2024-01-02 Monsanto Technology, Llc Soybean cultivar 01140108
US11696557B2 (en) 2021-08-31 2023-07-11 Monsanto Technology, Llc Soybean cultivar 04060955
US11700826B2 (en) 2021-08-31 2023-07-18 Monsanto Technology, Llc Soybean cultivar 02410315
US11825804B2 (en) 2021-09-28 2023-11-28 Monsanto Technology, Llc Soybean variety 01083877
US11819003B2 (en) 2021-09-28 2023-11-21 Monsanto Technology, Llc Soybean variety 01083835
US11825805B2 (en) 2021-09-28 2023-11-28 Monsanto Technology, Llc Soybean variety 01083845
US11730132B2 (en) 2021-09-28 2023-08-22 Monsanto Technology, Llc Soybean variety 01083701
US11856918B2 (en) 2021-09-28 2024-01-02 Monsanto Technology, Llc Soybean variety 01084034
US11903357B2 (en) 2021-09-28 2024-02-20 Monsanto Technology Llc Soybean variety 01083737
US11825806B2 (en) 2021-09-28 2023-11-28 Monsanto Technology, Llc Soybean variety 01083849
US11997978B2 (en) 2021-09-28 2024-06-04 Monsanto Technology Llc Soybean variety 01083711
US11707044B2 (en) 2021-09-28 2023-07-25 Monsanto Technology, Llc Soybean variety 01083999
US11985949B2 (en) * 2021-09-28 2024-05-21 Monsanto Technology Llc Soybean variety 01091555
US11917969B2 (en) 2021-09-28 2024-03-05 Monsanto Technology Llc Soybean variety 01083899
US11917968B2 (en) 2021-09-28 2024-03-05 Monsanto Technology Llc Soybean variety 01083684
US11744215B2 (en) 2021-09-28 2023-09-05 Monsanto Technology, Llc Soybean variety 01084068
US11917971B2 (en) 2021-09-29 2024-03-05 Monsanto Technology Llc Soybean variety 01091703
US11744217B2 (en) 2021-09-29 2023-09-05 Monsanto Technology, Llc Soybean variety 01091711
US11925161B2 (en) 2021-09-29 2024-03-12 Monsanto Technology Llc Soybean variety 01091628
US11758869B2 (en) 2021-09-29 2023-09-19 M.S. Technologies, L.L.C. Soybean variety 01091607
US11716957B2 (en) 2021-09-29 2023-08-08 Monsanto Technology, Llc Soybean variety 01091574
US11839192B2 (en) 2021-09-29 2023-12-12 Monsanto Technology, Llc Soybean variety 01091705
US11917970B2 (en) 2021-09-29 2024-03-05 Monsanto Technology Llc Soybean variety 01091697
US11930768B2 (en) 2021-09-29 2024-03-19 Monsanto Technology Llc Soybean variety 01091702
US11716958B2 (en) 2021-09-29 2023-08-08 Monsanto Technology, Llc Soybean variety 01091691
US11744216B2 (en) 2021-09-29 2023-09-05 Monsanto Technology, Llc Soybean variety 01091693
US12089555B2 (en) 2021-09-30 2024-09-17 Monsanto Technology Llc Soybean variety 01091725
US11930769B2 (en) 2021-09-30 2024-03-19 Monsanto Technology Llc Soybean variety 01091763
US11751529B2 (en) 2021-09-30 2023-09-12 M.S. Technologies, L.L.C. Soybean variety 01091734
US11744218B2 (en) 2021-09-30 2023-09-05 Monsanto Technology, Llc Soybean variety 01091749
US12004475B2 (en) 2021-09-30 2024-06-11 Monsanto Technology Llc Soybean variety 01091767
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US11963509B2 (en) 2021-10-01 2024-04-23 Monsanto Technology Llc Soybean variety 01091775
US11917973B2 (en) 2021-10-01 2024-03-05 Monsanto Technology Llc Soybean variety 01091785
US20230107744A1 (en) * 2021-10-01 2023-04-06 Monsanto Technology Llc Soybean variety 01091779
US11930770B2 (en) 2021-10-01 2024-03-19 Monsanto Technology Llc Soybean variety 01091765
US11917972B2 (en) 2021-10-01 2024-03-05 Monsanto Technology Llc Soybean variety 01091777
US11832578B2 (en) 2021-10-04 2023-12-05 Monsanto Technology, Llc Soybean variety 01091795
US11917974B2 (en) 2021-10-04 2024-03-05 Monsanto Technology, Llc Soybean variety 01091792
US11839193B2 (en) 2021-10-04 2023-12-12 Monsanto Technology, Llc Soybean variety 01091798
US12082547B2 (en) * 2021-10-04 2024-09-10 Monsanto Technology Llc Soybean variety 01091811
US11903358B2 (en) 2021-10-04 2024-02-20 Monsanto Technology Llc Soybean variety 01091805
US20230107769A1 (en) * 2021-10-04 2023-04-06 Monsanto Technology Llc Soybean variety 01091810
WO2024006698A2 (en) * 2022-06-27 2024-01-04 Monsanto Technology Llc Soybean transgenic event gm_csm63770 and methods for detection and uses thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040117870A1 (en) * 2000-11-30 2004-06-17 Guy Weyens T227-1 Flanking sequence
US7022896B1 (en) * 1997-04-04 2006-04-04 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
US20080120739A1 (en) * 2006-06-06 2008-05-22 Monsanto Technology Llc Method for selection of transformed cells
US20080166699A1 (en) * 2006-05-25 2008-07-10 George James Baley Method to identify disease resistant quantitative trait loci in soybean and compositions thereof
US20080305952A1 (en) * 2006-10-25 2008-12-11 Arnevik Cindy L Cropping systems for managing weeds
US20090199308A1 (en) * 2005-08-30 2009-08-06 Kimberly Zobrist Duff Transgenic plants with enhanced agronomic traits

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US200739A (en) * 1878-02-26 Improvement in barometers
US5094945A (en) 1983-01-05 1992-03-10 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
DE3765449D1 (en) 1986-03-11 1990-11-15 Plant Genetic Systems Nv PLANT CELLS RESISTED BY GENE TECHNOLOGY AND RESISTANT TO GLUTAMINE SYNTHETASE INHIBITORS.
US5276268A (en) 1986-08-23 1994-01-04 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
ATE213774T1 (en) 1990-06-25 2002-03-15 Monsanto Technology Llc GLYPHOSATE TOLERANT PLANTS
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US6069298A (en) * 1993-02-05 2000-05-30 Regents Of The University Of Minnesota Methods and an acetyl CoA carboxylase gene for conferring herbicide tolerance and an alteration in oil content of plants
US5850019A (en) 1996-08-06 1998-12-15 University Of Kentucky Research Foundation Promoter (FLt) for the full-length transcript of peanut chlorotic streak caulimovirus (PCLSV) and expression of chimeric genes in plants
US6040497A (en) 1997-04-03 2000-03-21 Dekalb Genetics Corporation Glyphosate resistant maize lines
KR100558838B1 (en) 1997-04-04 2006-03-10 보드 오브 리젠츠 오브 디 유니버시티 오브 네브라스카 Methods and Materials for the Preparation and Use of Transgenic Dicamba-Degrading Organisms
US7105724B2 (en) 1997-04-04 2006-09-12 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
US5914451A (en) 1998-04-06 1999-06-22 Monsanto Company Efficiency soybean transformation protocol
BRPI0007815B1 (en) 1999-01-14 2016-04-19 Monsanto Technology Llc soybean transformation process
ATE553202T1 (en) * 2003-01-31 2012-04-15 Monsanto Technology Llc GLYPHOSATE-TOLERANT ALUCERNE PLANTS AND METHOD FOR THE DETECTION THEREOF
US7157281B2 (en) 2003-12-11 2007-01-02 Monsanto Technology Llc High lysine maize compositions and event LY038 maize plants
AP2693A (en) * 2005-05-27 2013-07-16 Monsanto Technology Llc Soybean event MON89788 and methods for detection thereof
UA98770C2 (en) * 2006-05-26 2012-06-25 Монсанто Текнолоджи, Ллс Corn plant and seeds corresponding to trangenic event mon89034, and methods for its determination and using
AR061249A1 (en) * 2006-06-06 2008-08-13 Monsanto Technology Llc WEED CONTROL METHODS
UA94613C2 (en) * 2006-06-06 2011-05-25 Монсанто Текнолоджи Ллс Modified enzyme dmo and transgenic plant, producing it
US7855326B2 (en) * 2006-06-06 2010-12-21 Monsanto Technology Llc Methods for weed control using plants having dicamba-degrading enzymatic activity
US7884262B2 (en) * 2006-06-06 2011-02-08 Monsanto Technology Llc Modified DMO enzyme and methods of its use
US7951995B2 (en) * 2006-06-28 2011-05-31 Pioneer Hi-Bred International, Inc. Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof
EP2053916A2 (en) 2006-10-16 2009-05-06 Monsanto Technology, LLC Methods and compositions for improving plant health
US8207092B2 (en) 2006-10-16 2012-06-26 Monsanto Technology Llc Methods and compositions for improving plant health
CL2008000083A1 (en) 2007-01-12 2008-07-18 Monsanto Technology Llc Soc Or CRYSTALLIZED POLYPEPTIDE OF DICAMBA MONOOXIGENASA (BMD); MOLECULA THAT INCLUDES A UNION SURFACE FOR DICAMBA; VEGETABLE CELL THAT UNDERSTANDS DMO.
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
AR061248A1 (en) * 2007-02-26 2008-08-13 Monsanto Technology Llc CHLOROPLASTIC TRANSIT PEPTIDES FOR THE EFFICIENT ADDRESS OF DMO AND ITS USES
US8049071B2 (en) 2007-11-15 2011-11-01 Monsanto Technology Llc Soybean plant and seed corresponding to transgenic event MON87701 and methods for detection thereof
BRPI0908809A2 (en) 2008-02-15 2015-08-18 Monsanto Technology Llc Soybean and seed plant corresponding to transgenic event mon87769 and methods for its detection
PT2478000T (en) 2009-09-17 2018-07-17 Monsanto Technology Llc Soybean transgenic event mon 87708 and methods of use thereof
BR112012012404B1 (en) 2009-11-23 2019-03-06 Monsanto Technology Llc "AMPLICON RECONBINANT DNA Molecule, DNA Probe, DNA Molecule Pair METHOD FOR DETECTING THE PRESENCE OF A DNA MOLECULE AND DNA DETECTION KIT".
PT2575431T (en) 2010-06-04 2018-06-21 Monsanto Technology Llc Transgenic brassica event mon 88302 and methods of use thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7022896B1 (en) * 1997-04-04 2006-04-04 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
US20040117870A1 (en) * 2000-11-30 2004-06-17 Guy Weyens T227-1 Flanking sequence
US20090199308A1 (en) * 2005-08-30 2009-08-06 Kimberly Zobrist Duff Transgenic plants with enhanced agronomic traits
US20080166699A1 (en) * 2006-05-25 2008-07-10 George James Baley Method to identify disease resistant quantitative trait loci in soybean and compositions thereof
US20080120739A1 (en) * 2006-06-06 2008-05-22 Monsanto Technology Llc Method for selection of transformed cells
US20080305952A1 (en) * 2006-10-25 2008-12-11 Arnevik Cindy L Cropping systems for managing weeds

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [online] 16 February 2007 (2007-02-16), "GM_WBc0118A04.f GM_WBc Glycine max genomic clone GM_WBc0118A04 5', genomic survey sequence.", XP008153119, Database accession no. E1363554 *
DATABASE GENBANK [online] 8 December 2004 (2004-12-08), "RPCI42_145O22.TV RPCI-42 Bos taurus genomic clone RPCI42_145O22, genomic survey sequence.", XP008153116, Database accession no. CW906303 *
See also references of EP2478000A4 *

Cited By (340)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10561083B2 (en) 2009-11-23 2020-02-18 Monsanto Technology Llc Transgenic maize event MON 87427 and the relative development scale
US11441155B2 (en) 2009-11-23 2022-09-13 Monsanto Technology, Llc Transgenic maize event MON 87427 and the relative development scale
US9055743B2 (en) 2010-11-29 2015-06-16 Bayer Intellectual Property Gmbh Alpha, beta-unsaturated imines
WO2012072489A1 (en) 2010-11-29 2012-06-07 Bayer Cropscience Ag Alpha,beta-unsaturated imines
EP3103338A1 (en) 2010-12-01 2016-12-14 Bayer Intellectual Property GmbH Agent combinations comprising pyridylethyl benzamides and other agents
EP3092900A1 (en) 2010-12-01 2016-11-16 Bayer Intellectual Property GmbH Active ingredient combinations comprising pyridylethylbenzamides and other active ingredients
EP3103334A1 (en) 2010-12-01 2016-12-14 Bayer Intellectual Property GmbH Agent combinations comprising pyridylethyl benzamides and other agents
EP3103339A1 (en) 2010-12-01 2016-12-14 Bayer Intellectual Property GmbH Agent combinations comprising pyridylethyl benzamides and other agents
EP3103340A1 (en) 2010-12-01 2016-12-14 Bayer Intellectual Property GmbH Agent combinations comprising pyridylethyl benzamides and other agents
WO2012072696A1 (en) 2010-12-01 2012-06-07 Bayer Cropscience Ag Active ingredient combinations comprising pyridylethylbenzamides and other active ingredients
WO2012072660A1 (en) 2010-12-01 2012-06-07 Bayer Cropscience Ag Use of fluopyram for controlling nematodes in crops and for increasing yield
WO2012120105A1 (en) 2011-03-10 2012-09-13 Bayer Cropscience Ag Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds
EP3292761A1 (en) 2011-03-23 2018-03-14 Bayer Intellectual Property GmbH Active compound combinations
WO2012126938A2 (en) 2011-03-23 2012-09-27 Bayer Cropscience Ag Active compound combinations
EP3292760A1 (en) 2011-03-23 2018-03-14 Bayer Intellectual Property GmbH Active compound combinations
EP3295797A1 (en) 2011-03-23 2018-03-21 Bayer Intellectual Property GmbH Active compound combinations
CN103597079B (en) * 2011-03-30 2017-04-05 孟山都技术公司 Cotton transgenic event MON88701 and its using method
US10030277B2 (en) 2011-03-30 2018-07-24 Monsanto Technology Llc Cotton transgenic event MON 88701 and methods of use thereof
US10774341B2 (en) 2011-03-30 2020-09-15 Monsanto Technology Llc Cotton transgenic event MON 88701 and methods of use thereof
US9024115B2 (en) 2011-03-30 2015-05-05 Monsanto Technology Llc Cotton transgenic event MON 88701 and methods of use thereof
CN103597079A (en) * 2011-03-30 2014-02-19 孟山都技术公司 Cotton transgenic event MON 88701 and methods of use thereof
WO2012136581A1 (en) 2011-04-08 2012-10-11 Bayer Cropscience Ag Fungicide hydroximoyl-tetrazole derivatives
EP2997825A1 (en) 2011-04-22 2016-03-23 Bayer Intellectual Property GmbH Active compound combinations comprising a (thio)carboxamide derivative and a fungicidal compound
US9241493B2 (en) 2011-06-14 2016-01-26 Bayer Intellectual Property Gmbh Use of an enaminocarbonyl compound in combination with a biological control agent
WO2012171914A1 (en) 2011-06-14 2012-12-20 Bayer Intellectual Property Gmbh Use of an enaminocarbonyl compound in combination with a biological control agent
US9670496B2 (en) 2011-08-22 2017-06-06 Bayer Cropscience N.V. Methods and means to modify a plant genome
WO2013026740A2 (en) 2011-08-22 2013-02-28 Bayer Cropscience Nv Methods and means to modify a plant genome
US10538774B2 (en) 2011-08-22 2020-01-21 Basf Agricultural Solutions Seed, Us Llc Methods and means to modify a plant genome
EP2561759A1 (en) 2011-08-26 2013-02-27 Bayer Cropscience AG Fluoroalkyl-substituted 2-amidobenzimidazoles and their effect on plant growth
WO2013037717A1 (en) 2011-09-12 2013-03-21 Bayer Intellectual Property Gmbh Fungicidal 4-substituted-3-{phenyl[(heterocyclylmethoxy)imino]methyl}-1,2,4-oxadizol-5(4h)-one derivatives
WO2013037956A1 (en) 2011-09-16 2013-03-21 Bayer Intellectual Property Gmbh Use of 5-phenyl- or 5-benzyl-2 isoxazoline-3 carboxylates for improving plant yield
WO2013037958A1 (en) 2011-09-16 2013-03-21 Bayer Intellectual Property Gmbh Use of phenylpyrazolin-3-carboxylates for improving plant yield
WO2013037955A1 (en) 2011-09-16 2013-03-21 Bayer Intellectual Property Gmbh Use of acylsulfonamides for improving plant yield
WO2013050410A1 (en) 2011-10-04 2013-04-11 Bayer Intellectual Property Gmbh RNAi FOR THE CONTROL OF FUNGI AND OOMYCETES BY INHIBITING SACCHAROPINE DEHYDROGENASE GENE
WO2013075817A1 (en) 2011-11-21 2013-05-30 Bayer Intellectual Property Gmbh Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives
WO2013079566A2 (en) 2011-11-30 2013-06-06 Bayer Intellectual Property Gmbh Fungicidal n-bicycloalkyl and n-tricycloalkyl (thio)carboxamide derivatives
WO2013092519A1 (en) 2011-12-19 2013-06-27 Bayer Cropscience Ag Use of anthranilic acid diamide derivatives for pest control in transgenic crops
WO2013098146A1 (en) 2011-12-29 2013-07-04 Bayer Intellectual Property Gmbh Fungicidal 3-[(1,3-thiazol-4-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives
WO2013098147A1 (en) 2011-12-29 2013-07-04 Bayer Intellectual Property Gmbh Fungicidal 3-[(pyridin-2-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives
WO2013110591A1 (en) 2012-01-25 2013-08-01 Bayer Intellectual Property Gmbh Active compounds combination containing fluopyram bacillus and biologically control agent
WO2013110594A1 (en) 2012-01-25 2013-08-01 Bayer Intellectual Property Gmbh Active compound combinations containing fluopyram and biological control agent
WO2013127704A1 (en) 2012-02-27 2013-09-06 Bayer Intellectual Property Gmbh Active compound combinations containing a thiazoylisoxazoline and a fungicide
WO2013139949A1 (en) 2012-03-23 2013-09-26 Bayer Intellectual Property Gmbh Compositions comprising a strigolactame compound for enhanced plant growth and yield
WO2013153143A1 (en) 2012-04-12 2013-10-17 Bayer Cropscience Ag N-acyl- 2 - (cyclo) alkylpyrrolidines and piperidines useful as fungicides
WO2013156559A1 (en) 2012-04-20 2013-10-24 Bayer Cropscience Ag N-cycloalkyl-n-[(heterocyclylphenyl)methylene]-(thio)carboxamide derivatives
WO2013156560A1 (en) 2012-04-20 2013-10-24 Bayer Cropscience Ag N-cycloalkyl-n-[(trisubstitutedsilylphenyl)methylene]-(thio)carboxamide derivatives
EP2662360A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole indanyl carboxamides
EP2662362A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazole indanyl carboxamides
EP2662364A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazole tetrahydronaphthyl carboxamides
EP2662370A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole benzofuranyl carboxamides
EP2662363A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole biphenylcarboxamides
EP2662361A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazol indanyl carboxamides
WO2013167545A1 (en) 2012-05-09 2013-11-14 Bayer Cropscience Ag Pyrazole indanyl carboxamides
WO2013167544A1 (en) 2012-05-09 2013-11-14 Bayer Cropscience Ag 5-halogenopyrazole indanyl carboxamides
WO2013174836A1 (en) 2012-05-22 2013-11-28 Bayer Cropscience Ag Active compounds combinations comprising a lipo-chitooligosaccharide derivative and a nematicide, insecticidal or fungicidal compound
EP3360418A1 (en) 2012-05-30 2018-08-15 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
EP3205210A1 (en) 2012-05-30 2017-08-16 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide selected from inhibitors of the succinate dehydrogenase
EP3409120A1 (en) 2012-05-30 2018-12-05 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
EP3318128A2 (en) 2012-05-30 2018-05-09 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
EP3300603A2 (en) 2012-05-30 2018-04-04 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
EP3363289A2 (en) 2012-05-30 2018-08-22 Bayer CropScience Aktiengesellschaft Compositions comprising a biological control agent and an insecticide
EP3292764A2 (en) 2012-05-30 2018-03-14 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide selected from inhibitors of the respiratory chain at complex iii
EP3488700A1 (en) 2012-05-30 2019-05-29 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
EP3243387A2 (en) 2012-05-30 2017-11-15 Bayer CropScience Aktiengesellschaft Compositions comprising a biological control agent and an insecticide
EP3281526A1 (en) 2012-05-30 2018-02-14 Bayer CropScience Aktiengesellschaft Composition comprising a biological control agent and a fungicide
EP3424322A1 (en) 2012-07-31 2019-01-09 Bayer CropScience Aktiengesellschaft Compositions comprising a pesticidal terpene mixture and an insecticide
WO2014019983A1 (en) 2012-07-31 2014-02-06 Bayer Cropscience Ag Compositions comprising a pesticidal terpene mixture and an insecticide
EP3683307A2 (en) 2012-09-14 2020-07-22 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
EP3173477A1 (en) 2012-09-14 2017-05-31 Bayer Cropscience LP Hppd variants and methods of use
WO2014043435A1 (en) 2012-09-14 2014-03-20 Bayer Cropscience Lp Hppd variants and methods of use
WO2014056956A1 (en) 2012-10-11 2014-04-17 Bayer Cropscience Ag Use of n-phenylethylpyrazole carboxamide derivatives or salts thereof for resistance management of phytopathogenic fungi
EP2719280A1 (en) 2012-10-11 2014-04-16 Bayer CropScience AG Use of N-phenylethylpyrazole carboxamide derivatives or salts thereof for resistance management of phytopathogenic fungi
WO2014060518A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Method of plant growth promotion using carboxamide derivatives
WO2014060502A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Active compound combinations comprising carboxamide derivatives
WO2014060520A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Method for treating plants against fungi resistant to fungicides using carboxamide or thiocarboxamide derivatives
WO2014060519A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives
EP2735231A1 (en) 2012-11-23 2014-05-28 Bayer CropScience AG Active compound combinations
WO2014079789A1 (en) 2012-11-23 2014-05-30 Bayer Cropscience Ag Active compound combinations
WO2014082950A1 (en) 2012-11-30 2014-06-05 Bayer Cropscience Ag Ternary fungicidal mixtures
WO2014083089A1 (en) 2012-11-30 2014-06-05 Bayer Cropscience Ag Ternary fungicidal and pesticidal mixtures
WO2014083031A2 (en) 2012-11-30 2014-06-05 Bayer Cropscience Ag Binary pesticidal and fungicidal mixtures
WO2014083088A2 (en) 2012-11-30 2014-06-05 Bayer Cropscience Ag Binary fungicidal mixtures
WO2014083033A1 (en) 2012-11-30 2014-06-05 Bayer Cropsience Ag Binary fungicidal or pesticidal mixture
WO2014086759A2 (en) 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising biological control agents
WO2014086750A2 (en) 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide
WO2014086749A2 (en) 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide
WO2014086747A2 (en) 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide
WO2014086764A2 (en) 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide
WO2014086753A2 (en) 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising biological control agents
WO2014086758A2 (en) 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide
EP3318129A1 (en) 2012-12-03 2018-05-09 Bayer CropScience Aktiengesellschaft Method for pest control by applying a combination of paecilomyces lilacinus and fluopyram
WO2014086748A2 (en) 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide
WO2014090765A1 (en) 2012-12-12 2014-06-19 Bayer Cropscience Ag Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops
WO2014095826A1 (en) 2012-12-18 2014-06-26 Bayer Cropscience Ag Binary fungicidal and bactericidal combinations
WO2014095677A1 (en) 2012-12-19 2014-06-26 Bayer Cropscience Ag Difluoromethyl-nicotinic- tetrahydronaphtyl carboxamides
WO2014124379A1 (en) 2013-02-11 2014-08-14 Bayer Cropscience Lp Compositions comprising a streptomyces-based biological control agent and an insecticide
WO2014124368A1 (en) 2013-02-11 2014-08-14 Bayer Cropscience Lp Compositions comprising gougerotin and a fungicide
WO2014124369A1 (en) 2013-02-11 2014-08-14 Bayer Cropscience Lp Compositions comprising a streptomyces-based biological control agent and a fungicide
WO2014124373A1 (en) 2013-02-11 2014-08-14 Bayer Cropscience Lp Compositions comprising gougerotin and an insecticide
WO2014124375A1 (en) 2013-02-11 2014-08-14 Bayer Cropscience Lp Compositions comprising gougerotin and a biological control agent
WO2014124361A1 (en) 2013-02-11 2014-08-14 Bayer Cropscience Lp Compositions comprising a streptomyces-based biological control agent and another biological control agent
EP3626828A2 (en) 2013-03-07 2020-03-25 BASF Agricultural Solutions Seed US LLC Toxin genes and methods for their use
WO2014138339A2 (en) 2013-03-07 2014-09-12 Athenix Corp. Toxin genes and methods for their use
WO2014170364A1 (en) 2013-04-19 2014-10-23 Bayer Cropscience Ag Binary insecticidal or pesticidal mixture
WO2014170345A2 (en) 2013-04-19 2014-10-23 Bayer Cropscience Ag Method for improved utilization of the production potential of transgenic plants
WO2014177514A1 (en) 2013-04-30 2014-11-06 Bayer Cropscience Ag Nematicidal n-substituted phenethylcarboxamides
WO2014177582A1 (en) 2013-04-30 2014-11-06 Bayer Cropscience Ag N-(2-fluoro-2-phenethyl)carboxamides as nematicides and endoparasiticides
WO2014206953A1 (en) 2013-06-26 2014-12-31 Bayer Cropscience Ag N-cycloalkyl-n-[(bicyclylphenyl)methylene]-(thio)carboxamide derivatives
WO2015082586A1 (en) 2013-12-05 2015-06-11 Bayer Cropscience Ag N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives
WO2015082587A1 (en) 2013-12-05 2015-06-11 Bayer Cropscience Ag N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives
EP2885970A1 (en) 2013-12-21 2015-06-24 Bayer CropScience AG Fungicide compositions comprising compound I, at least one succinate dehydrogenase (SDH) inhibitor and at least one triazole fungicide
WO2015138394A2 (en) 2014-03-11 2015-09-17 Bayer Cropscience Lp Hppd variants and methods of use
US11987798B2 (en) 2014-03-20 2024-05-21 Monsanto Technology Llc Transgenic maize event MON 87419 and methods of use thereof
US11098321B2 (en) 2014-03-20 2021-08-24 Monsanto Technology Llc Transgenic maize event MON 87419 and methods of use thereof
US10113178B2 (en) 2014-03-20 2018-10-30 Monsanto Technology Llc Transgenic maize event MON 87419 and methods of use thereof
WO2015160618A1 (en) 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and a biological control agent
WO2015160619A1 (en) 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and a fungicide
WO2015160620A1 (en) 2014-04-16 2015-10-22 Bayer Cropscience Lp Compositions comprising ningnanmycin and an insecticide
WO2016166077A1 (en) 2015-04-13 2016-10-20 Bayer Cropscience Aktiengesellschaft N-cycloalkyl-n-(biheterocyclyethylene)-(thio)carboxamide derivatives
EP3097782A1 (en) 2015-05-29 2016-11-30 Bayer CropScience Aktiengesellschaft Methods for controlling phytopathogenic nematodes by combination of fluopyram and biological control agents
WO2016193073A1 (en) 2015-05-29 2016-12-08 Bayer Cropscience Aktiengesellschaft Methods for controlling phytopathogenic nematodes by combination of fluopyram and biological control agents
WO2017042259A1 (en) 2015-09-11 2017-03-16 Bayer Cropscience Aktiengesellschaft Hppd variants and methods of use
WO2018019676A1 (en) 2016-07-29 2018-02-01 Bayer Cropscience Aktiengesellschaft Active compound combinations and methods to protect the propagation material of plants
WO2018098214A1 (en) 2016-11-23 2018-05-31 Bayer Cropscience Lp Axmi669 and axmi991 toxin genes and methods for their use
WO2018114393A1 (en) 2016-12-19 2018-06-28 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018119361A1 (en) 2016-12-22 2018-06-28 Bayer Cropscience Lp Elite event ee-gm4 and methods and kits for identifying such event in biological samples
WO2018119336A1 (en) 2016-12-22 2018-06-28 Athenix Corp. Use of cry14 for the control of nematode pests
WO2018119364A1 (en) 2016-12-22 2018-06-28 Bayer Cropscience Lp Elite event ee-gm5 and methods and kits for identifying such event in biological samples
WO2018136604A1 (en) 2017-01-18 2018-07-26 Bayer Cropscience Lp Bp005 toxin gene and methods for its use
WO2018136611A1 (en) 2017-01-18 2018-07-26 Bayer Cropscience Lp Use of bp005 for the control of plant pathogens
WO2018153730A1 (en) 2017-02-21 2018-08-30 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018165091A1 (en) 2017-03-07 2018-09-13 Bayer Cropscience Lp Hppd variants and methods of use
WO2018184970A1 (en) 2017-04-07 2018-10-11 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018188962A1 (en) 2017-04-11 2018-10-18 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018195256A1 (en) 2017-04-21 2018-10-25 Bayer Cropscience Lp Method of improving crop safety
WO2018202487A1 (en) 2017-05-04 2018-11-08 Basf Se Substituted 5-(haloalkyl)-5-hydroxy-isoxazoles for combating phytopathogenic fungi
WO2018202491A1 (en) 2017-05-04 2018-11-08 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2018219797A1 (en) 2017-06-02 2018-12-06 Basf Se Substituted oxadiazoles for combating phytopathogenic fungi
WO2018234139A1 (en) 2017-06-19 2018-12-27 Basf Se 2-[[5-(trifluoromethyl)-1,2,4-oxadiazol-3-yl]aryloxy](thio)acetamides for combating phytopathogenic fungi
WO2019025250A1 (en) 2017-08-04 2019-02-07 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019038042A1 (en) 2017-08-21 2019-02-28 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019052932A1 (en) 2017-09-18 2019-03-21 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019068811A1 (en) 2017-10-06 2019-04-11 Bayer Aktiengesellschaft Compositions comprising fluopyram and tioxazafen
WO2019083808A1 (en) 2017-10-24 2019-05-02 Basf Se Improvement of herbicide tolerance to hppd inhibitors by down-regulation of putative 4-hydroxyphenylpyruvate reductases in soybean
WO2019083810A1 (en) 2017-10-24 2019-05-02 Basf Se Improvement of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (hppd) inhibitors by down-regulation of hppd expression in soybean
WO2019101511A1 (en) 2017-11-23 2019-05-31 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
US11834466B2 (en) 2017-11-30 2023-12-05 5Metis, Inc. Benzoxaborole compounds and formulations thereof
WO2019121143A1 (en) 2017-12-20 2019-06-27 Basf Se Substituted cyclopropyl derivatives
WO2019137995A1 (en) 2018-01-11 2019-07-18 Basf Se Novel pyridazine compounds for controlling invertebrate pests
WO2019145221A1 (en) 2018-01-29 2019-08-01 BASF Agro B.V. New agrochemical formulations
WO2019154663A1 (en) 2018-02-07 2019-08-15 Basf Se New pyridine carboxamides
WO2019154665A1 (en) 2018-02-07 2019-08-15 Basf Se New pyridine carboxamides
WO2019166257A1 (en) 2018-03-01 2019-09-06 BASF Agro B.V. Fungicidal compositions of mefentrifluconazole
WO2019219464A1 (en) 2018-05-15 2019-11-21 Basf Se Substituted trifluoromethyloxadiazoles for combating phytopathogenic fungi
WO2019224092A1 (en) 2018-05-22 2019-11-28 Basf Se Pesticidally active c15-derivatives of ginkgolides
WO2019233863A1 (en) 2018-06-04 2019-12-12 Bayer Aktiengesellschaft Herbicidally active bicyclic benzoylpyrazoles
US12098159B2 (en) 2018-08-18 2024-09-24 5Metis, Inc. Solid forms of substituted benzoxaborole and compositions thereof
US11236115B2 (en) 2018-08-18 2022-02-01 5Metis, Inc. Solid forms of substituted benzoxaborole and compositions thereof
US11066424B2 (en) 2018-08-18 2021-07-20 Boragen, Inc. Solid forms of substituted benzoxaborole and compositions thereof
US11560393B2 (en) 2018-08-18 2023-01-24 5Metis, Inc. Solid forms of substituted benzoxaborole and compositions thereof
EP3613736A1 (en) 2018-08-22 2020-02-26 Basf Se Substituted glutarimide derivatives
EP3628158A1 (en) 2018-09-28 2020-04-01 Basf Se Pesticidal mixture comprising a mesoionic compound and a biopesticide
WO2020064480A1 (en) 2018-09-28 2020-04-02 Basf Se Pesticidal mixture comprising a mesoionic compound and a biopesticide
AU2019362776C1 (en) * 2018-10-16 2022-10-13 Monsanto Technology Llc Brassica Event MON94100 and methods of use thereof
CN115667516A (en) * 2018-10-16 2023-01-31 孟山都技术公司 Brassica event MON94100 and methods of use thereof
US11098323B2 (en) 2018-10-16 2021-08-24 Monsanto Technology Llc Brassica event MON94100 and methods of use thereof
AU2019362776B2 (en) * 2018-10-16 2022-01-06 Monsanto Technology Llc Brassica Event MON94100 and methods of use thereof
EP3867386A4 (en) * 2018-10-16 2022-07-13 Monsanto Technology LLC Brassica event mon94100 and methods of use thereof
US11976290B2 (en) 2018-10-16 2024-05-07 Monsanto Technology Llc Brassica event MON94100 and methods of use thereof
WO2020081464A1 (en) * 2018-10-16 2020-04-23 Monsanto Technology Llc Brassica event mon94100 and methods of use thereof
WO2020083662A1 (en) 2018-10-23 2020-04-30 Basf Se Tricyclic pesticidal compounds
WO2020083733A1 (en) 2018-10-24 2020-04-30 Basf Se Pesticidal compounds
EP3643705A1 (en) 2018-10-24 2020-04-29 Basf Se Pesticidal compounds
EP3670501A1 (en) 2018-12-17 2020-06-24 Basf Se Substituted [1,2,4]triazole compounds as fungicides
WO2020144308A1 (en) 2019-01-11 2020-07-16 Basf Se Crystalline forms of 1-(1,2-dimethylpropyl)-n-ethyl-5-methyl-n-pyridazin-4-yl-pyrazole-4-carboxamide
EP3696177A1 (en) 2019-02-12 2020-08-19 Basf Se Heterocyclic compounds for the control of invertebrate pests
RU2802940C2 (en) * 2019-03-28 2023-09-05 БАЙОСИРИЗ ЭлЭлСи Transgenic object ind-00410-5 of soya
WO2020231751A1 (en) 2019-05-10 2020-11-19 Bayer Cropscience Lp Active compound combinations
WO2020239517A1 (en) 2019-05-29 2020-12-03 Basf Se Mesoionic imidazolium compounds and derivatives for combating animal pests
WO2020244970A1 (en) 2019-06-06 2020-12-10 Basf Se New carbocyclic pyridine carboxamides
WO2020244969A1 (en) 2019-06-06 2020-12-10 Basf Se Pyridine derivatives and their use as fungicides
WO2020244968A1 (en) 2019-06-06 2020-12-10 Basf Se Fungicidal n-(pyrid-3-yl)carboxamides
WO2021013561A1 (en) 2019-07-19 2021-01-28 Basf Se Pesticidal pyrazole and triazole derivatives
EP3766879A1 (en) 2019-07-19 2021-01-20 Basf Se Pesticidal pyrazole derivatives
EP3769623A1 (en) 2019-07-22 2021-01-27 Basf Se Mesoionic imidazolium compounds and derivatives for combating animal pests
WO2021013721A1 (en) 2019-07-22 2021-01-28 Bayer Aktiengesellschaft 5-amino substituted pyrazoles and triazoles as pest control agents
WO2021013720A1 (en) 2019-07-23 2021-01-28 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021013719A1 (en) 2019-07-23 2021-01-28 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021022069A1 (en) 2019-08-01 2021-02-04 Bayer Cropscience Lp Method of improving cold stress tolerance and crop safety
EP3701796A1 (en) 2019-08-08 2020-09-02 Bayer AG Active compound combinations
WO2021058659A1 (en) 2019-09-26 2021-04-01 Bayer Aktiengesellschaft Rnai-mediated pest control
WO2021063735A1 (en) 2019-10-02 2021-04-08 Basf Se New bicyclic pyridine derivatives
WO2021064075A1 (en) 2019-10-02 2021-04-08 Bayer Aktiengesellschaft Active compound combinations comprising fatty acids
WO2021063736A1 (en) 2019-10-02 2021-04-08 Basf Se Bicyclic pyridine derivatives
WO2021069569A1 (en) 2019-10-09 2021-04-15 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021069567A1 (en) 2019-10-09 2021-04-15 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021089673A1 (en) 2019-11-07 2021-05-14 Bayer Aktiengesellschaft Substituted sulfonyl amides for controlling animal pests
WO2021097162A1 (en) 2019-11-13 2021-05-20 Bayer Cropscience Lp Beneficial combinations with paenibacillus
WO2021099303A1 (en) 2019-11-18 2021-05-27 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021099271A1 (en) 2019-11-18 2021-05-27 Bayer Aktiengesellschaft Active compound combinations comprising fatty acids
WO2021105091A1 (en) 2019-11-25 2021-06-03 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021155084A1 (en) 2020-01-31 2021-08-05 Pairwise Plants Services, Inc. Suppression of shade avoidance response in plants
WO2021165195A1 (en) 2020-02-18 2021-08-26 Bayer Aktiengesellschaft Heteroaryl-triazole compounds as pesticides
EP3708565A1 (en) 2020-03-04 2020-09-16 Bayer AG Pyrimidinyloxyphenylamidines and the use thereof as fungicides
WO2021209490A1 (en) 2020-04-16 2021-10-21 Bayer Aktiengesellschaft Cyclaminephenylaminoquinolines as fungicides
WO2021211926A1 (en) 2020-04-16 2021-10-21 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
WO2021213978A1 (en) 2020-04-21 2021-10-28 Bayer Aktiengesellschaft 2-(het)aryl-substituted condensed heterocyclic derivatives as pest control agents
WO2021219513A1 (en) 2020-04-28 2021-11-04 Basf Se Pesticidal compounds
EP3903583A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iii
EP3903584A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors iv
EP3903582A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ii
EP3903581A1 (en) 2020-04-28 2021-11-03 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors i
WO2021224220A1 (en) 2020-05-06 2021-11-11 Bayer Aktiengesellschaft Pyridine (thio)amides as fungicidal compounds
WO2021224323A1 (en) 2020-05-06 2021-11-11 Bayer Aktiengesellschaft Novel heteroaryl-triazole compounds as pesticides
WO2021228734A1 (en) 2020-05-12 2021-11-18 Bayer Aktiengesellschaft Triazine and pyrimidine (thio)amides as fungicidal compounds
EP3909950A1 (en) 2020-05-13 2021-11-17 Basf Se Heterocyclic compounds for the control of invertebrate pests
WO2021233861A1 (en) 2020-05-19 2021-11-25 Bayer Aktiengesellschaft Azabicyclic(thio)amides as fungicidal compounds
WO2021247477A1 (en) 2020-06-02 2021-12-09 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
WO2021245087A1 (en) 2020-06-04 2021-12-09 Bayer Aktiengesellschaft Heterocyclyl pyrimidines and triazines as novel fungicides
WO2021249995A1 (en) 2020-06-10 2021-12-16 Bayer Aktiengesellschaft Azabicyclyl-substituted heterocycles as fungicides
WO2021249800A1 (en) 2020-06-10 2021-12-16 Basf Se Substituted [1,2,4]triazole compounds as fungicides
WO2021257775A1 (en) 2020-06-17 2021-12-23 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
WO2021255071A1 (en) 2020-06-18 2021-12-23 Bayer Aktiengesellschaft 3-(pyridazin-4-yl)-5,6-dihydro-4h-1,2,4-oxadiazine derivatives as fungicides for crop protection
WO2021255118A1 (en) 2020-06-18 2021-12-23 Bayer Aktiengesellschaft Composition for use in agriculture
WO2021255091A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazoles and their derivatives as fungicides
WO2021255169A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazole pyrimidines as fungicides
WO2021255170A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazole pyrimidines as fungicides
WO2021255089A1 (en) 2020-06-19 2021-12-23 Bayer Aktiengesellschaft 1,3,4-oxadiazole pyrimidines and 1,3,4-oxadiazole pyridines as fungicides
WO2021259997A1 (en) 2020-06-25 2021-12-30 Bayer Animal Health Gmbh Novel heteroaryl-substituted pyrazine derivatives as pesticides
EP3929189A1 (en) 2020-06-25 2021-12-29 Bayer Animal Health GmbH Novel heteroaryl-substituted pyrazine derivatives as pesticides
WO2022002818A1 (en) 2020-07-02 2022-01-06 Bayer Aktiengesellschaft Heterocyclene derivatives as pest control agents
EP3939961A1 (en) 2020-07-16 2022-01-19 Basf Se Strobilurin type compounds and their use for combating phytopathogenic fungi
WO2022017836A1 (en) 2020-07-20 2022-01-27 BASF Agro B.V. Fungicidal compositions comprising (r)-2-[4-(4-chlorophenoxy)-2-(trifluoromethyl)phenyl]-1- (1,2,4-triazol-1-yl)propan-2-ol
EP3945089A1 (en) 2020-07-31 2022-02-02 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors v
WO2022033991A1 (en) 2020-08-13 2022-02-17 Bayer Aktiengesellschaft 5-amino substituted triazoles as pest control agents
EP3960727A1 (en) 2020-08-28 2022-03-02 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors vi
WO2022043559A2 (en) 2020-08-31 2022-03-03 Basf Se Yield improvement
WO2022053453A1 (en) 2020-09-09 2022-03-17 Bayer Aktiengesellschaft Azole carboxamide as pest control agents
WO2022058327A1 (en) 2020-09-15 2022-03-24 Bayer Aktiengesellschaft Substituted ureas and derivatives as new antifungal agents
EP3970494A1 (en) 2020-09-21 2022-03-23 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors viii
EP3974414A1 (en) 2020-09-25 2022-03-30 Bayer AG 5-amino substituted pyrazoles and triazoles as pesticides
WO2022089969A1 (en) 2020-10-27 2022-05-05 BASF Agro B.V. Compositions comprising mefentrifluconazole
WO2022090071A1 (en) 2020-11-02 2022-05-05 Basf Se Use of mefenpyr-diethyl for controlling phytopathogenic fungi
WO2022090069A1 (en) 2020-11-02 2022-05-05 Basf Se Compositions comprising mefenpyr-diethyl
WO2022106304A1 (en) 2020-11-23 2022-05-27 BASF Agro B.V. Compositions comprising mefentrifluconazole
WO2022128524A1 (en) 2020-12-14 2022-06-23 Basf Se Sulfoximine pesticides
EP3915971A1 (en) 2020-12-16 2021-12-01 Bayer Aktiengesellschaft Phenyl-s(o)n-phenylamidines and the use thereof as fungicides
WO2022129200A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft Use of dhodh inhibitor for controlling resistant phytopathogenic fungi in crops
WO2022129188A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft 1,2,4-oxadiazol-3-yl pyrimidines as fungicides
WO2022129190A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft (hetero)aryl substituted 1,2,4-oxadiazoles as fungicides
WO2022129196A1 (en) 2020-12-18 2022-06-23 Bayer Aktiengesellschaft Heterobicycle substituted 1,2,4-oxadiazoles as fungicides
EP4036083A1 (en) 2021-02-02 2022-08-03 Bayer Aktiengesellschaft 5-oxy substituted heterocycles as pesticides
WO2022173885A1 (en) 2021-02-11 2022-08-18 Pairwise Plants Services, Inc. Methods and compositions for modifying cytokinin oxidase levels in plants
EP4043444A1 (en) 2021-02-11 2022-08-17 Basf Se Substituted isoxazoline derivatives
WO2022182834A1 (en) 2021-02-25 2022-09-01 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture in plants
WO2022207494A1 (en) 2021-03-30 2022-10-06 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2022207496A1 (en) 2021-03-30 2022-10-06 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2022233758A1 (en) 2021-05-03 2022-11-10 Basf Se Additives for enhancing the pesticidal effectiveness of pesticidal microorganisms
WO2022233777A1 (en) 2021-05-06 2022-11-10 Bayer Aktiengesellschaft Alkylamide substituted, annulated imidazoles and use thereof as insecticides
WO2022238391A1 (en) 2021-05-12 2022-11-17 Bayer Aktiengesellschaft 2-(het)aryl-substituted condensed heterocycle derivatives as pest control agents
EP4091451A1 (en) 2021-05-17 2022-11-23 BASF Agro B.V. Compositions comprising mefentrifluconazole
WO2022243109A1 (en) 2021-05-18 2022-11-24 Basf Se New substituted quinolines as fungicides
WO2022243107A1 (en) 2021-05-18 2022-11-24 Basf Se New substituted pyridines as fungicides
WO2022243111A1 (en) 2021-05-18 2022-11-24 Basf Se New substituted pyridines as fungicides
WO2022263285A1 (en) 2021-06-14 2022-12-22 Basf Se Yield improvement by gene combinations
WO2022266271A1 (en) 2021-06-17 2022-12-22 Pairwise Plants Services, Inc. Modification of growth regulating factor family transcription factors in soybean
WO2022271892A1 (en) 2021-06-24 2022-12-29 Pairwise Plants Services, Inc. Modification of hect e3 ubiquitin ligase genes to improve yield traits
WO2023278651A1 (en) 2021-07-01 2023-01-05 Pairwise Plants Services, Inc. Methods and compositions for enhancing root system development
EP4119547A1 (en) 2021-07-12 2023-01-18 Basf Se Triazole compounds for the control of invertebrate pests
WO2023011957A1 (en) 2021-08-02 2023-02-09 Basf Se (3-quinolyl)-quinazoline
WO2023011958A1 (en) 2021-08-02 2023-02-09 Basf Se (3-pirydyl)-quinazoline
WO2023019188A1 (en) 2021-08-12 2023-02-16 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
WO2023017120A1 (en) 2021-08-13 2023-02-16 Bayer Aktiengesellschaft Active compound combinations and fungicide compositions comprising those
WO2023023496A1 (en) 2021-08-17 2023-02-23 Pairwise Plants Services, Inc. Methods and compositions for modifying cytokinin receptor histidine kinase genes in plants
EP4140986A1 (en) 2021-08-23 2023-03-01 Basf Se Pyrazine compounds for the control of invertebrate pests
WO2023025682A1 (en) 2021-08-25 2023-03-02 Bayer Aktiengesellschaft Novel pyrazinyl-triazole compounds as pesticides
EP4140995A1 (en) 2021-08-27 2023-03-01 Basf Se Pyrazine compounds for the control of invertebrate pests
WO2023034731A1 (en) 2021-08-30 2023-03-09 Pairwise Plants Services, Inc. Modification of ubiquitin binding peptidase genes in plants for yield trait improvement
EP4144739A1 (en) 2021-09-02 2023-03-08 Bayer Aktiengesellschaft Anellated pyrazoles as parasiticides
WO2023034891A1 (en) 2021-09-02 2023-03-09 Pairwise Plants Services, Inc. Methods and compositions for improving plant architecture and yield traits
EP4151631A1 (en) 2021-09-20 2023-03-22 Basf Se Heterocyclic compounds for the control of invertebrate pests
WO2023049720A1 (en) 2021-09-21 2023-03-30 Pairwise Plants Services, Inc. Methods and compositions for reducing pod shatter in canola
WO2023060028A1 (en) 2021-10-04 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023060152A2 (en) 2021-10-07 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023072670A1 (en) 2021-10-28 2023-05-04 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors x
WO2023072671A1 (en) 2021-10-28 2023-05-04 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors ix
WO2023078915A1 (en) 2021-11-03 2023-05-11 Bayer Aktiengesellschaft Bis(hetero)aryl thioether (thio)amides as fungicidal compounds
WO2023099445A1 (en) 2021-11-30 2023-06-08 Bayer Aktiengesellschaft Bis(hetero)aryl thioether oxadiazines as fungicidal compounds
EP4194453A1 (en) 2021-12-08 2023-06-14 Basf Se Pyrazine compounds for the control of invertebrate pests
WO2023108035A1 (en) 2021-12-09 2023-06-15 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
EP4198033A1 (en) 2021-12-14 2023-06-21 Basf Se Heterocyclic compounds for the control of invertebrate pests
EP4198023A1 (en) 2021-12-16 2023-06-21 Basf Se Pesticidally active thiosemicarbazone compounds
WO2023110932A1 (en) 2021-12-16 2023-06-22 Basf Se Pesticidally active thiosemicarbazone compounds
WO2023147526A1 (en) 2022-01-31 2023-08-03 Pairwise Plants Services, Inc. Suppression of shade avoidance response in plants
WO2023148028A1 (en) 2022-02-01 2023-08-10 Globachem Nv Methods and compositions for controlling pests
WO2023148036A1 (en) 2022-02-01 2023-08-10 Globachem Nv Methods and compositions for controlling pests in soybean
WO2023156402A1 (en) 2022-02-17 2023-08-24 Basf Se Pesticidally active thiosemicarbazone compounds
WO2023156270A1 (en) 2022-02-18 2023-08-24 Basf Se Coumarin synthesis and uses thereof
WO2023168217A1 (en) 2022-03-02 2023-09-07 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
EP4238971A1 (en) 2022-03-02 2023-09-06 Basf Se Substituted isoxazoline derivatives
WO2023192838A1 (en) 2022-03-31 2023-10-05 Pairwise Plants Services, Inc. Early flowering rosaceae plants with improved characteristics
WO2023196886A1 (en) 2022-04-07 2023-10-12 Pairwise Plants Services, Inc. Methods and compositions for improving resistance to fusarium head blight
WO2023205714A1 (en) 2022-04-21 2023-10-26 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
WO2023215704A1 (en) 2022-05-02 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for enhancing yield and disease resistance
WO2023213670A1 (en) 2022-05-03 2023-11-09 Bayer Aktiengesellschaft Crystalline forms of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine
WO2023213626A1 (en) 2022-05-03 2023-11-09 Bayer Aktiengesellschaft Use of (5s)-3-[3-(3-chloro-2-fluorophenoxy)-6-methylpyridazin-4-yl]-5-(2-chloro-4-methylbenzyl)-5,6-dihydro-4h-1,2,4-oxadiazine for controlling unwanted microorganisms
WO2023215809A1 (en) 2022-05-05 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture and/or improving plant yield traits
WO2024006679A1 (en) 2022-06-27 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
WO2024006791A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024006792A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024018016A1 (en) 2022-07-21 2024-01-25 Syngenta Crop Protection Ag Crystalline forms of 1,2,4-oxadiazole fungicides
WO2024028243A1 (en) 2022-08-02 2024-02-08 Basf Se Pyrazolo pesticidal compounds
WO2024030984A1 (en) 2022-08-04 2024-02-08 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
WO2024036240A1 (en) 2022-08-11 2024-02-15 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024033374A1 (en) 2022-08-11 2024-02-15 Syngenta Crop Protection Ag Novel arylcarboxamide or arylthioamide compounds
WO2024054880A1 (en) 2022-09-08 2024-03-14 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
EP4342885A1 (en) 2022-09-20 2024-03-27 Basf Se N-(3-(aminomethyl)-phenyl)-5-(4-phenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-amine derivatives and similar compounds as pesticides
WO2024068838A1 (en) 2022-09-28 2024-04-04 Syngenta Crop Protection Ag Fungicidal compositions
WO2024068519A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2024068518A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-heteroaryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2024068520A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
EP4295688A1 (en) 2022-09-28 2023-12-27 Bayer Aktiengesellschaft Active compound combination
WO2024068517A1 (en) 2022-09-28 2024-04-04 Bayer Aktiengesellschaft 3-(hetero)aryl-5-chlorodifluoromethyl-1,2,4-oxadiazole as fungicide
WO2024068837A1 (en) 2022-09-28 2024-04-04 Syngenta Crop Protection Ag Agricultural methods
EP4361126A1 (en) 2022-10-24 2024-05-01 Basf Se Use of strobilurin type compounds for combating phytopathogenic fungi containing an amino acid substitution f129l in the mitochondrial cytochrome b protein conferring resistance to qo inhibitors xv
WO2024100069A1 (en) 2022-11-08 2024-05-16 Syngenta Crop Protection Ag Microbiocidal pyridine derivatives
WO2024104822A1 (en) 2022-11-16 2024-05-23 Basf Se Substituted tetrahydrobenzodiazepine as fungicides
WO2024104815A1 (en) 2022-11-16 2024-05-23 Basf Se Substituted benzodiazepines as fungicides
WO2024104818A1 (en) 2022-11-16 2024-05-23 Basf Se Substituted benzodiazepines as fungicides
WO2024104823A1 (en) 2022-11-16 2024-05-23 Basf Se New substituted tetrahydrobenzoxazepine
EP4385327A1 (en) 2022-12-15 2024-06-19 Kimitec Group S.L. Biopesticide composition and method for controlling and treating broad spectrum of pests and diseases in plants
WO2024126688A1 (en) 2022-12-15 2024-06-20 Kimitec Biogroup S.L Biopesticide composition and method for controlling and treating broad spectrum of pests and diseases in plants
WO2024137438A2 (en) 2022-12-19 2024-06-27 BASF Agricultural Solutions Seed US LLC Insect toxin genes and methods for their use
EP4389210A1 (en) 2022-12-21 2024-06-26 Basf Se Heteroaryl compounds for the control of invertebrate pests
WO2024165343A1 (en) 2023-02-08 2024-08-15 Basf Se New substituted quinoline compounds for combatitng phytopathogenic fungi
WO2024173622A1 (en) 2023-02-16 2024-08-22 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
WO2024182658A1 (en) 2023-03-02 2024-09-06 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
WO2024186950A1 (en) 2023-03-09 2024-09-12 Pairwise Plants Services, Inc. Modification of brassinosteroid signaling pathway genes for improving yield traits in plants
WO2024194038A1 (en) 2023-03-17 2024-09-26 Basf Se Substituted pyridyl/pyrazidyl dihydrobenzothiazepine compounds for combatting phytopathogenic fungi
EP4455137A1 (en) 2023-04-24 2024-10-30 Basf Se Pyrimidine compounds for the control of invertebrate pests

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