WO2011027291A1 - Cartouche de détecteur pourvue d'une couche de couverture soluble - Google Patents

Cartouche de détecteur pourvue d'une couche de couverture soluble Download PDF

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Publication number
WO2011027291A1
WO2011027291A1 PCT/IB2010/053898 IB2010053898W WO2011027291A1 WO 2011027291 A1 WO2011027291 A1 WO 2011027291A1 IB 2010053898 W IB2010053898 W IB 2010053898W WO 2011027291 A1 WO2011027291 A1 WO 2011027291A1
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WO
WIPO (PCT)
Prior art keywords
cover layer
optical structure
cartridge
sensor device
light beam
Prior art date
Application number
PCT/IB2010/053898
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English (en)
Inventor
Dominique M. Bruls
Original Assignee
Koninklijke Philips Electronics N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips Electronics N.V. filed Critical Koninklijke Philips Electronics N.V.
Publication of WO2011027291A1 publication Critical patent/WO2011027291A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/11—Filling or emptying of cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G01N2021/4173—Phase distribution
    • G01N2021/4193—Phase distribution using a PSD
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G01N21/43—Refractivity; Phase-affecting properties, e.g. optical path length by measuring critical angle

Definitions

  • the invention relates to a cartridge comprising a detection surface, to a sensor device for processing such a cartridge, and to a method for detecting a dissolvable cover layer on a detection surface. By detection of the dissolving of the cover layer, wetting of the detection surface may be determined.
  • the WO 2009/001289 Al discloses on optical procedure for determining the wetting grade of a surface. This comprises detection of an intensity change in a reflected light beam that is caused by a liquid contacting the surface. The known procedure requires that a sample liquid can immediately contact the surface of the carrier.
  • the invention relates to a cartridge for a sensor device, wherein the term "cartridge” shall denote an exchangeable element or unit with which a sample can be provided to a sensor device for processing.
  • the cartridge will usually be a disposable component which is used only once for a single sample.
  • the cartridge comprises the following components:
  • a sample chamber which can be filled with a (liquid or gaseous) sample that shall be hold by the cartridge, the sample chamber having at least one dedicated (piece of) surface which will in the following be called “detection surface” for purposes of reference.
  • the detection surface is an interior surface of the sample chamber, constituting the interface towards a sample (if present).
  • the sample chamber is typically an empty cavity or a cavity filled with some substance that may absorb a sample substance; it may be an open cavity, a closed cavity, or a cavity connected to other cavities by fluid connection channels.
  • optical structure that is formed in the aforementioned detection surface.
  • an “optical structure” shall denote any configuration that optically deviates from the smooth (planar), homogeneous constitution of the "normal” detection surface and that can hence affect the passage of light rays by scattering, refraction, reflection, diffraction and/or absorption.
  • the optical structure may particularly correspond to a three-dimensional configuration (topology) of the detection surface.
  • a dissolvable cover layer that is disposed on the aforementioned optical structure.
  • the cover layer is formed by a (solid) material which can dissolve in an appropriate fluid, particularly a water-soluble material. Due to its immediate contact to the optical structure, the cover layer affects the optical effect of the optical structure.
  • the invention further relates to a sensor device for processing a cartridge of the kind described above, i.e. for manipulating and/or examining a sample that is provided by the cartridge.
  • the cartridge may optionally be considered as a component of the sensor device or not.
  • the sensor device comprises the following components:
  • the input light beam will reach the optical structure through the (transparent) material of the cartridge.
  • the light source may for example be a laser or a light emitting diode (LED), optionally provided with some optics for shaping and directing the input light beam.
  • Said interaction between the input light and the optical structure may particularly comprise a reflection, refraction, diffraction, or scattering, but also the largely unaffected passage of input light through the optical structure.
  • the evaluation unit for determining the presence or absence of a cover layer on the optical structure from the results of the aforementioned detection.
  • the evaluation unit may be realized by dedicated electronic hardware, digital data processing hardware with appropriate software, or a mixture of both.
  • the invention further relates to a method for detecting with a sensor device a dissolvable cover layer on a "detection surface", particularly on the detection surface of a cartridge of the kind described above.
  • the method comprises the following steps, which are preferably executed at least once in the listed sequence: Irradiating an optical structure, which is formed in said detection surface, with an input light beam.
  • the cartridge, the sensor device, and the method described above have in common that an optical structure is provided on the detection surface and that this optical structure is (at least initially) covered by a dissolvable cover layer.
  • the presence or absence of such a cover layer may then optically be detected with the help of an output light beam that comes from the optical structure and that is affected by the optical structure and the medium in contact therewith.
  • the dissolvable cover layer may particularly be removed from the optical structure by dissolving it in an adjacent (sample) liquid, the presence or absence of the cover layer will often correspond to the presence or absence of a (sample) liquid in the sample chamber. Detection of the (absence of a) cover layer is then tantamount to the detection of said liquid.
  • the cover layer comprises a transparent material, i.e. a material that absorbs (in a given spectral range) less than about 70 %, preferably less than about 30 %, most preferably less than about 10 % of a light beam that passes it.
  • the cover layer will be transparent in the spectral range of the light that is used to investigate it, for example of the input light beam and/or output light beam mentioned above.
  • a transparent cover layer allows to affect the passage of light through the optical structure by refraction, i.e. the presence of the cover layer changes the optical properties (e.g. scattering/transmission properties) of the optical structure.
  • the cover layer comprises a (typically transparent) material having (in a given spectral range, e.g. that of visible light) a similar refractive index as the material the optical structure is built of.
  • similarity means that the refractive indices differ by less than about 30 %, preferably less than about 15 %, most preferably less than about 5 % from each other, in which the refractive index of the cover layer preferably matches or is higher than the refractive index of the cartridge material.
  • the refractive indices of the optical structure and the cover layer are approximately (or exactly) the same, light rays can pass the interface between these components substantially without (hardly any) refraction.
  • the optical effect of the optical structure is hence neutralized by the cover layer. This will typically yield a large, well detectable difference with respect to the situation that no cover layer is disposed on the optical structure and the optical structure can exhibit its refractive properties.
  • the input light beam is preferably directed towards the optical structure in such a way that it reaches the detection surface under conditions of total internal reflection (with respect to the media typically present in the sample chamber, e.g. air and/or water-like liquids). Total internal reflection will then occur at the surface of the cover layer (if present) in the aforementioned case, i.e. when the cover layer has a similar index of refraction as the optical structure.
  • the output light beam may hence comprise (approximately) all the light of the input light beam, giving rise to a high, well detectable signal difference, w.r.t. the case where no cover layer is present and the optical structure can refract light out of the output beam, hence lowering the optical signal obtained from the output beam.
  • the presence or absence of a cover layer can influence an output light beam in many different, detectable ways.
  • the cover layer and the optical structure have such a form and/or refraction index that at least a part of the light of the input light beam is directed as the output light beam towards the light detector if the cover layer is present. The presence of this light will then reveal the presence of the cover layer.
  • at least a part of the light of the input light beam may be directed as the output light beam towards the light detector if the cover layer is NOT present. In this case the presence of this light reveals the absence of the cover layer.
  • Parts of the surface of the cover layer that are not in contact to the optical structure preferably have a topology that is different from the topology of the optical structure. Hence the effects these topologies have on passing light rays will be different, too.
  • the surface of the cover layer is smooth (e.g. planar). This will typically yield a maximal contrast to an interaction with the (non-smooth) optical structure.
  • the cover layer may comprise a sugar, e.g. sucrose or dextrose, a salt, a buffer component, a pH optimizing agent or the like as a constituting material.
  • a sugar e.g. sucrose or dextrose
  • a salt e.g. sodium bicarbonate
  • a buffer component e.g. sodium bicarbonate
  • a pH optimizing agent or the like e.g. sodium bicarbonate
  • a cover layer from such materials on the detection surface is particularly advantageous in bioassays.
  • biomolecules e.g. antibodies
  • the optical structure on the detection surface may be designed in many different ways.
  • the optical structure comprises a plurality of elevations, for example spikes or peaks and/or of angels.
  • the elevations or angels may be arranged in a regular or an irregular pattern.
  • the size of the elevations or angles typically ranges between 100 nm and 2 microns, with an aspect ratio that is preferably in the range of 1 or higher (meaning that the elevations are "sharp" features).
  • the elevations are preferably chosen in such a way that "mirror- like" areas are suppressed as much as possible, i.e. areas that are substantially parallel to the plane of the detection surface are avoided as much as possible. This is for example achieved in embodiments in which more than 50 %, preferably more than 75 %, most preferably more than 90 % of the area of the optical structure are inclined (i.e. non-parallel) with respect to the detection surface.
  • the angle of inclination of these area- fractions with respect to the detection surface may typically be larger than 10°, preferably larger than 45°.
  • the optical structure comprises an alignment element that can be localized in an image of the detection surface whether a cover layer is present or not.
  • an image sensor for example a camera
  • the alignment element can always be seen, allowing to particularly localize those parts of the optical structure that have to be evaluated for clues about the presence or absence of a cover layer.
  • the alignment element may be realized in different ways, for example by light absorbing optical markers on the detection surface.
  • the alignment element comprises a ridge.
  • a ridge can readily be produced with the same means as the rest of the optical structure.
  • the alignment element is preferably located at the border of the optical structure, most preferably encircling it at least partially.
  • the optically active area of the optical structure can then readily be determined.
  • this alignment element comprises structures that exceed the optical structures (used for the cover layer detection) w.r.t. their height. This means that the height of structures inside the alignment elements is chosen in such a way, that they are too high to be covered by the cover layer material.
  • the light detector of the sensor device is preferably adapted to allow for position dependent measurements. This may for example be achieved by a segmented light detector.
  • the light detector may be adapted to generate an image of the detection surface.
  • the light detector may for example comprise a CCD or CMOS chip as it is known from imaging devices like digital cameras.
  • Position dependent measurements or even the generation of an image of the detection surface has the advantage that the complete surface can in parallel be surveyed and that different processes can be evaluated at various positions on the detection surface.
  • the presence or absence of a cover layer on the optical structure may affect any characteristic feature of the output light beam, for example its spatial or spectral composition.
  • the affected feature is the amount of light in the output light beam (for example expressed by the intensity of the output light beam).
  • the presence or absence of a cover layer on the optical structure can then be determined by comparing this amount of light with a given, predetermined threshold.
  • the presence of a cover layer may particularly be inferred from a high intensity of the output light beam that is due to a (total internal) reflection of an input light beam at the (smooth) surface of the cover layer, while the absence of the cover layer may correspond to a low intensity of the output light beam due to scattering at the free optical structure.
  • the optical structure beneath the cover layer may be pretreated, i.e. be treated before the cover layer is deposited on the optical structure.
  • the pretreatment may for example comprise (RF) plasma treatment, ⁇ -ray irradiation, and/or buffer washing.
  • the sensor device typically serves some particular purpose to which the detection of a cover layer is only an auxiliary means.
  • the sensor device may particularly comprise at least one sensor unit for detecting the presence of target particles at the detection surface.
  • the "target particles” may especially comprise specific biological molecules or structures, for example complexes, cell fractions or cells.
  • the target particles may be or comprise label particles (e.g. atoms, molecules, complexes, nanoparticles, microparticles etc.) that have some property (e.g. optical density, magnetic susceptibility, electric charge, fluorescence, or radioactivity) which can readily be detected.
  • the sensor unit that detects target particles may optionally be an optical, magnetic, mechanical, acoustic, thermal and/or electrical sensor unit.
  • a magnetic sensor unit may particularly comprise a coil, Hall sensor, planar Hall sensor, flux gate sensor, SQUID (Superconducting Quantum Interference Device), magnetic resonance sensor, magneto- restrictive sensor, or magneto-resistive sensor of the kind described in the WO 2005/010543 Al or WO 2005/010542 A2, especially a GMR (Giant Magneto Resistance), a TMR (Tunnel Magneto Resistance), or an AMR (Anisotropic Magneto Resistance).
  • An optical sensor unit may particularly be adapted to detect variations in an output light beam that arise from a frustrated total internal reflection due to target particles at a sensing surface (cf.
  • the optical components that are used to determine the presence or absence of a cover layer may simultaneously be applied as a sensor unit for detecting the presence of target particles at the detection surface.
  • the sensor device may optionally further comprise a magnetic field generator, for example an electromagnet, for generating a magnetic field in the sample chamber of a cartridge when it has been inserted into the sensor device.
  • a magnetic field generator for example an electromagnet
  • magnetic particles for example superparamagnetic beads serving as labels for biological target molecules, can be manipulated by magnetic forces. They can for example be pulled towards the detection surface to accelerate binding processes or be repelled therefrom to break non-specific bindings.
  • the detection surface may particularly be (locally) coated with capture molecules to which target components of a sample can specifically bind.
  • the invention further relates to the use of the cartridge and/or the sensor device described above for molecular diagnostics, biological sample analysis, chemical sample analysis, food analysis, and/or forensic analysis.
  • Molecular diagnostics may for example be accomplished with the help of magnetic beads or fluorescent particles that are directly or indirectly attached to target molecules.
  • Fig. 1 schematically shows a sensor device according to the present
  • Fig. 2 shows the setup of Figure 1 after the cover layer has been dissolved
  • Fig. 3 shows exemplary measurements of light intensity when a cover layer is dissolved from different optical structures
  • Fig. 4 shows a perspective view onto an optical structure on the detection surface of a cartridge (left) and an enlarged view thereof (right).
  • Like reference numbers in the Figures refer to identical or similar components.
  • an optical biosensor that allows to detect the binding of target particles (e.g. drugs-of-abuse, cTroponin-I, PTH etc.) to the surface of a carrier by means of frustrated total internal reflection (FTIR).
  • target particles e.g. drugs-of-abuse, cTroponin-I, PTH etc.
  • FTIR frustrated total internal reflection
  • magnetic particles it is known to use magnetic particles to label target components in a sample, said magnetic particles allowing to actively move components by magnetic forces for purposes of process acceleration and stringency testing.
  • presence of a cover layer means that the cartridge is dry, while absence of the cover layer means that the latter has been dissolved due to presence of fluid, i.e. the cartridge is wet and wetting of the sensor surface has occurred.
  • sucrose As a material of the cover layer, sucrose can be used. This has the advantage to provide a proper protection of sensitive coatings (printed biospots) on the detection surface.
  • a sucrose layer has a refractive index n that is more or less equal (with n > 1.5 or even larger) to the refractive index of a typical optical cartridge material, for example optical-grade plastics like polystyrene, Topas, Zenox/Zenor (having a refractive index in the range of 1.51 to 1.58).
  • a typical optical cartridge material for example optical-grade plastics like polystyrene, Topas, Zenox/Zenor (having a refractive index in the range of 1.51 to 1.58).
  • this structure can be seen as a dark feature in an FTIR image as all light is being diffracted in all directions. If this structure is covered or buried by a sucrose layer, the scattering properties of the optical structure are inhibited.
  • the optical structure is hardly visible anymore in the FTIR image. This is as if the optical structure is not present at all and normal total internal reflection will occur between the interface of the sucrose layer and the air. If a sample fluid is added, the sucrose layer will however dissolve and the optical structure will be functional again, i.e. can be seen as a "dark" structure in an FTIR image.
  • FIG. 1 schematically illustrates in a side view a sensor device 50 with a cartridge 10 which are designed according to the above principles.
  • the cartridge 10 is typically a disposable device that may be produced from transparent plastic by injection moulding. It comprises a transparent body 15 with a detection surface 12 above which a sample chamber 11 extends.
  • the sample chamber 11 has an inlet 13 and an outlet 14 for filling it with some sample fluid during its use.
  • an optical structure 20 is formed which is symbolized in the Figure by a plurality of angles or elevations 21. As illustrated in this schematic representation, it is preferred that a large fraction (ideally 100 %) of the facets in the optical structure 20 are inclined with respect to the detection surface 12.
  • elevations 21 are bordered by an alignment element in the form of a ridge 22. It should be noted that the Figure depicts the optical structure 20 not to scale and neglects zones on the detection surface 12 where other measurements take place.
  • the optical structure 20 as well as the rest of the detection surface 12 are covered by a dissolvable cover layer 30, for example a layer of sucrose.
  • the sensor device 50 which may receive the disposable cartridge 10, comprises means for making optical investigations at the detection surface 12 of an inserted cartridge 10.
  • the sensor device 50 comprises a light source 51 for emitting an input light beam LI towards the detection surface 12, (inter alia) illuminating the optical structure 20.
  • the sensor device 50 comprises a light detector 52 that receives an output light beam L2 which originates from light of the input light beam LI that was reflected at the optical structure 20 and/or at the surface 31 of the cover layer 30.
  • the light detector 52 may comprise any suitable sensor or plurality of sensor units by which light of a given spectrum can be detected, for example photodiodes, photo resistors, photocells, or a photo multiplier tube.
  • FIG. 1 shows the setup for a dry sample chamber 11, i.e. when the cover layer 30 is present as originally fabricated
  • Figure 2 shows the setup after a sample liquid with target particles 1 has filled the sample chamber 11.
  • the target particles 1 may particularly comprise superparamagnetic beads that can be manipulated with a
  • the cover layer 30 which is assumed to be soluble in the sample liquid (e.g. being water-soluble), has completely dissolved, exposing the detection surface 12 with the optical structure 20 to the sample liquid. This has a crucial effect on the behavior of the input light beam LI at the detection surface:
  • the input light beam LI passed the optical structure 20 nearly unaffected because the refractive index of the cover layer 30 is assumed to be nearly the same as the refractive index of the cartridge material 15.
  • the output light beam L2 hence originates in Figure 1 from a (total internal) reflection of the input light beam LI at the surface 31 of the cover layer 30.
  • This output light beam L2 is therefore a bright beam, comprising (approximately) all the light of the input light beam LI .
  • Figure 3 shows measurement signals S (in relative units, normalized to 100 % at the beginning of the measurements) over time t that were observed for different types of optical structures when a sample liquid is introduced into an initially dry sample chamber 11.
  • a sharp, large drop in optical signal S is observed, as the processes at the optical structure 20 change from reflecting (due to the presence of the sucrose layer 30) to diffracting.
  • a threshold S th in software it is thus possible to detect very accurately whether and when fluid is inserted into the sample chamber 11, i.e. the moment ti at which the sucrose layer 30 dissolves in the fluid.
  • the used cartridges 10 are preferably made by means of injection moulding.
  • an optically flat insert may be used in the injection mould.
  • the top surface of this insert can be made out of NiP.
  • FIB Focus Ion Beam
  • FIB Focus Ion Beam
  • After moulding, a negative of this diffraction structure is obtained in the plastic part.
  • An example of such a milled diffraction structure 20 is shown in Figure 4 in a complete view (left) and an enlarged detail (right).
  • this optical structure 20 can be used as sucrose detector.
  • the depth of the features 21 is typically in the range of 2 to 4 micron.
  • microstructures are direct diamond milling, electrochemical etching, plasma etching, laser ablation etc., i.e. methods that can create "micro -roughness".
  • an additional ring or rim 22 is added, which is rather deep (> 2.5 micron) and has very steep walls (90 degrees). This ring cannot entirely be covered by sucrose. In this way, the ring is also visible in an FTIR image, even when the sucrose layer 30 is present. Hence it is possible to predict on forehand where the optical structure 20 will be, and which area(s) should be monitored for detection purposes.
  • a typical sucrose layer 30 can be made by spraying, pipetting, or local application of a sucrose solution.
  • some detergents like CHAPS (3-(3- cholamidopropyl)dimethylamonio-l-propanesulfonate) may be added to the sucrose solution.
  • Typical values for the sucrose solution are 0.005 % CHAPS and 2 % sucrose.
  • the overall thickness of the sucrose layer is in the order of about 500 nm to about 1 micron. At the position of the optical structure, some accumulation of sucrose appears to occur, which results in a layer that more or less covers the entire optical structure.

Abstract

La présente invention concerne un dispositif détecteur (50) et une cartouche (10) associée permettant de détecter la présence ou l'absence d'une couche de couverture (30) sur une surface de détection (12) de la cartouche (10). La présence de la couche de couverture révèle typiquement l'absence de fluide échantillon dans la chambre de réaction de la cartouche, puisque la couche de couverture est soluble dans le fluide échantillon. A cette fin, une structure optique (20) est formée dans la surface de détection (12). La détection d'une couche de couverture (30) sur la structure optique (20) est ensuite réalisée par évaluation d'un faisceau de lumière de sortie (L2) provenant d'une interaction au niveau de la structure optique (20). De préférence, la couche de couverture (30) présente le même indice de réfraction que le matériau de la structure optique (20). En particulier, elle peut comprendre du saccharose.
PCT/IB2010/053898 2009-09-07 2010-08-31 Cartouche de détecteur pourvue d'une couche de couverture soluble WO2011027291A1 (fr)

Applications Claiming Priority (2)

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EP09169587.4 2009-09-07
EP09169587 2009-09-07

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Cited By (5)

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WO2013062540A1 (fr) * 2011-10-26 2013-05-02 Hewlett-Packard Development Company, L.P. Appareil pour utilisation dans une application de détection ayant une couverture destructible
WO2014013372A1 (fr) * 2012-07-18 2014-01-23 Koninklijke Philips N.V. Traitement d'un fluide d'échantillonnage contenant des composants cibles
WO2015121359A1 (fr) * 2014-02-13 2015-08-20 Koninklijke Philips N.V. Détection de mouillage sans marqueurs
US10466163B2 (en) 2011-04-28 2019-11-05 Koninklijke Philips N.V. Concurrently evaluating assays with optical inhomogeneities
CN111122400A (zh) * 2018-10-30 2020-05-08 宁波方太厨具有限公司 一种油烟检测装置及油烟检测方法

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EP2771658A4 (fr) * 2011-10-26 2015-04-15 Hewlett Packard Development Co Appareil pour utilisation dans une application de détection ayant une couverture destructible
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WO2015121359A1 (fr) * 2014-02-13 2015-08-20 Koninklijke Philips N.V. Détection de mouillage sans marqueurs
CN106507682A (zh) * 2014-02-13 2017-03-15 皇家飞利浦有限公司 没有标记物的润湿检测
CN111122400A (zh) * 2018-10-30 2020-05-08 宁波方太厨具有限公司 一种油烟检测装置及油烟检测方法

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