WO2011007551A1

WO2011007551A1 - Sugar alcohol-containing antibacterial agent for inhibiting proliferation of periodontal disease-causing bacteria

Info

Publication number: WO2011007551A1
Application number: PCT/JP2010/004530
Authority: WO
Inventors: 児玉悠史; 樋口裕明; 成瀬敦; 桜井孝治
Original assignee: 株式会社ロッテ
Priority date: 2009-07-14
Filing date: 2010-07-13
Publication date: 2011-01-20
Also published as: JP2011020935A; TW201117804A; KR20120042968A; JP5710111B2; CN102470113A

Abstract

Disclosed is an antibacterial agent having ameliorating and prophylactic effect on periodontal diseases and dental caries, which is characterized by the inhibitory effect of a sugar alcohol on proliferation of periodontal disease-causing bacteria and dental caries-causing bacteria. Specifically disclosed is an antibacterial agent for inhibiting proliferation of periodontal disease-causing bacteria, which contains a sugar alcohol, in particular lactitol or maltitol, as an active ingredient.

Description

[Name of invention determined by ISA based on Rule 37.2] Antimicrobial agent for periodontal pathogen growth inhibition containing sugar alcohol

The present invention relates to an antibacterial agent comprising sugar alcohols, particularly lactol and maltitol.

Sugar alcohol is low in calories, has non-cariogenic properties, and is used in a wide range of products such as foods, cosmetics, pharmaceuticals, and health foods from the viewpoint of preventing tooth decay and lifestyle-related diseases. In the oral cavity, the growth inhibitory action against the causative bacteria of dental caries is widely known, but many other usefulness are still unclear.

In the present invention, focusing on the usefulness of a new sugar alcohol related to periodontal pathogens that cause periodontal disease, the antibacterial activity of sugar alcohols and the inhibition of periodontal pathogen growth were evaluated.

There are various prior literatures regarding the antibacterial action of sugar alcohols.

Non-patent document 1 reports the effect of xylitol on the growth inhibition of P. gingivalis. However, sugar alcohols other than xylitol have not been reported at all regarding antibacterial or growth inhibition against periodontal pathogens, except for causative bacteria and Candida.

Patent Document 1 discloses a preventive or therapeutic agent for periodontal disease. In this document, lactic acid bacteria are mixed and inoculated with P. gingivalis in a medium containing sugars that can be assimilated by these bacteria, thereby suppressing the growth of P. gingivalis compared to the medium alone. In addition, the comparison of the proliferation of the culture medium containing saccharides and the culture medium not containing is not performed. In the Examples, glucose, lactose, and sucrose are used as sugars, and only P. gingivalis is used as a test strain. Furthermore, claim 2 separately describes xylitol, erythritol, and maltitol as sugars, and Prevotella intermedia, Actinobacillus actinomycetemcomitans, Candida, and mutans as control periodontal pathogens in the embodiment of the invention. However, there is no report of antibacterial action against periodontal pathogens by sugar alcohol alone.

Patent Document 2 discloses a composition for preventing and / or treating oral disease. By adding xylitol to the medium containing the culture filtrate of lactic acid bacteria, the growth of P. gingivalis, F. nucleatum, P. intermedia, and Actinobacillus actinomycetemcomitans is suppressed compared to the culture filtrate alone condition. Note that the addition of xylitol alone to the medium has little effect on the growth of these bacteria. The growth of P. gingivalis is also suppressed by adding erythritol or maltitol. However, there is no report of antibacterial action against periodontal pathogens by sugar alcohol alone.

Patent Document 3 discloses a live bacterial preparation containing lactic acid bacteria as an active ingredient and a food containing lactic acid bacteria. When erythritol was added to the basal medium to a final concentration of 5% and Streptococcus mutans was cultured for 24 hours, the number of viable cells was suppressed by 75% and the amount of insoluble glucan formed was suppressed by 60% compared to the condition where erythritol was not added. Yes. Moreover, when lactic acid bacteria are cultured simultaneously, the degree of suppression is further increased. According to claim 11, sugar alcohol contains only erythritol in the composition. However, there is no report of antibacterial action against periodontal pathogens by sugar alcohol alone.

Thus, in the prior literature, it was found that a sugar alcohol was added to the medium of lactic acid bacteria to suppress the growth of periodontal pathogens, except for causative bacteria, Staphylococcus aureus, Candida, There were no reports on the growth of periodontal pathogens under the condition of sugar alcohol alone.

JP 2003-171292 A Special table 02/080946 Special table 03/082027

It is an object of the present invention to provide an antibacterial agent that is effective in improving and preventing periodontal disease and cough, characterized by the growth inhibitory action of periodontal pathogens and crush-causing bacteria by lactitol and maltitol. To do.

Sugar alcohol is used in a wide range of products such as foods, cosmetics, pharmaceuticals and health foods, and its anti-proliferative action against causative bacteria in the oral cavity is widely known, but other usefulness has not been elucidated. Many. When periodontal pathogens were cultured in a medium containing lactitol or maltitol, it was confirmed that the growth was significantly inhibited as compared with a medium not containing lactitol or maltitol. Moreover, it was suggested that this effect is not bactericidal but may be bacteriostatic.

Since the present invention has an antibacterial action against periodontal pathogens, as a preparation such as a mouthwash, a toothpaste, an inhalant, a troche, etc., chewing gum, candy, tablet candy, gummy jelly, biscuits, It can be used and consumed on a daily basis as foods such as chocolate and other confectionery, sherbet and beverages, and is extremely effective in improving and preventing periodontal disease and touch.

It is a graph which shows the influence of sugar alcohol (xylitol) on the proliferation of F. nucleatum. It is a graph which shows the influence of sugar alcohol (erythritol) on the proliferation of F. nucleatum. It is a graph which shows the influence of sugar alcohol (lactitol) on the proliferation of F. nucleatum. It is a graph which shows the influence of the sugar alcohol (maltitol) on the proliferation of F. nucleatum. It is a graph which shows the influence of sugar alcohol (xylitol) on the proliferation of P. gingivalis. It is a graph which shows the influence of sugar alcohol (erythritol) on the proliferation of P. gingivalis. It is a graph which shows the influence of the sugar alcohol (lactitol) on the proliferation of P.gingivalis. It is a graph which shows the influence of sugar alcohol (maltitol) on the proliferation of P. gingivalis.

One embodiment of the present invention is an antibacterial agent for periodontal pathogen growth inhibition containing sugar alcohol as an active ingredient.

A further embodiment of the present invention is an antibacterial agent for inhibiting periodontal pathogen growth containing lactitol or maltitol as an active ingredient.

Another embodiment of the present invention is a mouthwash, a toothpaste, an inhalant, and a troche made of an antibacterial agent for inhibiting periodontal pathogen growth containing lactitol or maltitol as an active ingredient.

Still another embodiment of the present invention is a chewing gum, candy, tablet confectionery, gummy jelly, biscuits, chocolate and other confectionery, sherbet, containing an antibacterial agent for inhibiting periodontal pathogen growth containing lactitol or maltitol as an active ingredient , Food such as beverages.

Hereinafter, the present invention will be described in detail by way of specific examples, but the present invention is not limited thereto.

Example 1
The antibacterial test of sugar alcohol was performed as follows.

1-1. Test sample preparation method Using 2% ethanol, the final medium concentration is 8% for xylitol, erythritol, lactitol, maltitol, the final medium concentration is 800 ppm for thymol, and the final medium concentration is 200 ppm for zinc chloride. Each was prepared and a 2-fold dilution series was prepared with 2% ethanol.

1-2. Test strains As the periodontal pathogens, Porphyromonas gingivalis ATCC33277 (hereinafter abbreviated as P. gingivalis), Fusobacterium nucleatum ATCC25586 (hereinafter abbreviated as F.nucleatum), Prevotella intermedia ATCC25611 (hereinafter abbreviated as P.intermedia), Prevotella nigrescens
JCM6322 strain (hereinafter abbreviated as P. nigrescens), Treponema denticola
ATCC35405 strain (hereinafter abbreviated as T.denticola), Tannerella forsythia
JCM10827 strain (hereinafter abbreviated as T. forsythia) was used. In addition, among oral tongue bacteria, oral bacteria with a high percentage in tongue coating are Prevotella melaninogenica JCM6325 strain (hereinafter abbreviated as P. melaninogenica), Veillonella parvula JCM12972 strain (hereinafter abbreviated as V. parvula), and Veillonella dispar ATCC17748 strain. (Hereinafter abbreviated as V.dispar).

1-3. Media P.gingivalis, F.nucleatum, P.intermedia, P.nigrescens, P.melaninogenica are Tripticase soy supplemented with Yeast extract (3.0 g / l), Hemin (5.0 mg / l), Menadione (0.5 mg / l) Using broth, the cells were cultured under anaerobic conditions (10% CO ₂ , 10% H ₂ , 80% N ₂ ) at 37 ° C. V.parvula and V.dispar are Trypticase (5.0g / l), Yeast Extract (3.0g / l), Sodium lactate (9.0g / l), Sodium thioglycollate (0.75g / l), Tween80 (1.0g / l) , Glucose (1.0 g / l) added medium was adjusted to pH 7.5 with K ₂ CO ₂ and cultured under the same conditions. T. forsythia was prepared by sterilizing Brain heart infusion broth supplemented with Yeast extract (5.0 g / l), Hemin (5.0 mg / l) and Menadione (0.5 mg / l) at 120 ° C. for 15 minutes, and then L-cysteine ( 1.0 g / l), N-acetylneuraminic acid (10.0 mg / l), and Fetal Bovine Serum (50 ml / l) were added under the same conditions. T. denticola was cultured under the same conditions using TYGVS medium.

1-4. Determination of antibacterial property The antibacterial property test used a liquid dilution method in which dilution was performed in a 2-fold dilution series. That is, a test sample solution that has been diluted 2 times is added to a 96-well plate (the volume of each well is 100 μl), and the bacterial solution cultured in the above 1-3 medium is added to the same medium at a concentration of 5% or less. After dilution and mixing, 100 μl of this was added to a 96-well plate. In addition, as a control, add only 100 μl of medium without bacterial solution, and perform anaerobic culture (10% CO ₂ , 10% H ₂ , 80% N ₂ ) for 24 to 48 hours, using a spectrophotometer The OD ₆₆₀ value was measured. The concentration at which the OD ₆₆₀ value was comparable to that of the control and the growth of bacteria was not observed with the naked eye was defined as the minimum growth inhibitory concentration (hereinafter abbreviated as MIC).

1-5. Results Antibacterial tests were conducted for the purpose of confirming the antibacterial activity of various sugar alcohols against bad breath causing bacteria.

Table 1 shows the MIC of each test strain. All test samples showed no antibacterial activity against P. nigrescens, P. melaninogenica, V. parvula, T. forsythia at concentrations of 8% or less (thymol 800 ppm or less). Although antibacterial activity was not obtained with thymol against T. denticola, an MIC of 8% was confirmed for all four sugar alcohols used in the test. Conversely, thymol showed a MIC of 400 ppm for V.dispar, but sugar alcohol did not show antibacterial activity. In addition, the activity against P. gingivalis was weak compared to 25 ppm for zinc chloride and 200 ppm for thymol, but weak antibacterial activity was confirmed for all sugar alcohols. Although weak antibacterial property does not completely inhibit the growth of bacteria, it shows an OD ₆₆₀ value that is about half that of other concentrations, and it indicates a state in which the growth of bacteria is slightly recognized by the naked eye. Zinc chloride was tested for antibacterial activity only against P. gingivalis. Although it was less active against F. nucleatum compared to 200 ppm of thymol, xylitol and erythritol showed an MIC of 8%. Although antibacterial activity was not confirmed with thymol against P.intermedia, erythritol showed an MIC of 8%.

From the above results, it was clarified that sugar alcohol exhibits fungus-specific antibacterial properties. P.gingivalis, F.nucleatum, P.intermedia, and T.denticola are all major pathogens of periodontal disease and are involved in their development and progression. Sugar alcohol showed antibacterial activity against these pathogens, but did not show antibacterial activity against oral bacteria (P. melaninogenica, V. parvula, V. dispar), which have a high proportion of tongue coating among the tongue lichen localization bacteria. It has been reported that Veillonella is detected higher in young healthy volunteers than in patients with periodontal disease. The fact that sugar alcohols did not exhibit antibacterial properties for these strains as normal bacteria in the oral cavity of healthy people is considered to be a desirable result as a food additive. As for P. gingivalis, zinc chloride, which acts to inhibit bacterial metabolism, showed an extremely low MIC, which is thought to be due to inhibition of bacterial growth due to metabolic inhibition.

Δ: Weak antibacterial, ×: Antibacterial is not confirmed at all concentrations,
ND: No Data
Sugar alcohol concentration: 0.0078%-8%
Thymol concentration: 0.78ppm-800ppm
Zinc chloride concentration: 0.195ppm to 200ppm

(Example 2)
A sugar alcohol growth inhibition test was performed as follows.

2-1. Test Sample Preparation Method For xylitol, erythritol, lactitol and maltitol, the final medium concentration was adjusted to 5% or 10%.

2-2. As the test strain, F. nucleatum and P. gingivalis described in the test strain of Example 1 were used.

2-3. Medium P.gingivalis, F.nucleatum both Yeast extract (3.0 g / l), Hemin (5.0 mg / l), Tripticase soy broth supplemented with Menadione (0.5 mg / l), under anaerobic conditions at 37 ° C ( 10% CO ₂ , 10% H ₂ , 80% N ₂ ). When measuring the growth curve, sugar alcohol was added to the above medium to a final concentration of 5% or 10%, dissolved, and then sterilized at 120 ° C. for 15 minutes.

2-4. Measurement of Growth Curve 100 μl of the bacterial solution cultured in the above 2-3 medium until the logarithmic growth phase was added to 20 ml of a medium containing 5% or 10% of each sugar alcohol. As a control, 20 ml of a normal medium containing no sugar alcohol was added with 100 μl of the bacterial solution. Anaerobic culture (10% CO ₂ , 10% H ₂ , 80% N ₂ ) was performed for 40 to 47 hours, and OD ₆₆₀ value was measured with a spectrophotometer during this time.

2-5. Results In the antibacterial test, antibacterial activity was observed against 4 strains such as P. gingivalis, F. nucleatum, etc., suggesting the possibility that sugar alcohol could inhibit the growth of these strains. In order to confirm this possibility, the growth curves of P. gingivalis and F. nucleatum were measured in a medium containing sugar alcohol. The growth curves of F. nucleatum are shown in FIGS. 1A, 1B, 1C and 1D, and the growth curves of P. gingivalis are shown in FIGS. 2A, 2B, 2C and 2D. Each sugar alcohol was tested at n = 2 (denoted as 1 and 2 in the figure), and the control represents a sugar alcohol-free medium.

For F. nucleatum, xylitol and erythritol showed particularly strong growth inhibition. This result correlates with the result of antibacterial test against F. nucleatum. Both sugar alcohols showed OD ₆₆₀ values lower than the control at the time of culturing 44 hours at 5% and 10% concentrations. Lactitol and maltitol showed the same growth as the control at 5% concentration, but showed a lower OD ₆₆₀ value at 10% concentration than the control at 44 hours after culturing.

Lactitol and maltitol showed particularly strong growth inhibition against P. gingivalis.

In both sugar alcohols at 5% and 10% concentrations, no growth was observed even after 40 hours of culturing, and the culture solution was transparent even when visually confirmed. In addition, although the effects of xylitol and erythritol varied, they showed weak growth inhibition. Both sugar alcohols showed bacterial growth from around 20 hours of culture, and showed an OD ₆₆₀ value similar to that of the control after 46 hours. For lactitol and maltitol, there was a possibility that the bacteria were killed due to the influence of water activity, so apply 10% lactitol and 10% maltitol culture solution 100 μl after 40 hours of culture to the blood plate medium, The cells were cultured for 10 days under anaerobic conditions (10% CO ₂ , 10% H ₂ , 80% N ₂ ) at 37 ° C. As a result, 83 colonies were confirmed under 10% lactitol conditions and 308 colonies under 10% maltitol conditions, suggesting that both sugar alcohols may act bacteriostatically rather than bactericidally.
Variations are seen in 10% xylitol, and reproducibility needs to be confirmed, but xylitol and erythritol are thought to have the effect of delaying the time to transition to the logarithmic growth phase.

Next, a mouthwash containing a periodontal pathogen growth inhibitory antibacterial agent comprising the sugar alcohol of the present invention, toothpaste, spray for bad breath, troche, chewing gum, candy, tablet confectionery, gummy jelly, and beverage were produced by conventional methods. . Their formulations are shown below. Note that the scope of the present invention is not limited by these.

(Example 3)
A mouthwash was prepared according to the following formulation.
Ethanol 2.0% by weight
Lactitol 10.0
Fragrance 1.0
Water remaining
100.0

Example 4
A toothpaste was produced according to the following formulation.
Calcium carbonate 40.0% by weight
Glycerin 10.0
Maltitol 20.0
Carboxymethylcellulose 2.0
Sodium ralyl sulfate 2.0
Fragrance 1.0
Saccharin 0.1
Chlorhexidine 0.01
Water remaining
100.0

(Example 5)
A spray for halitosis was produced according to the following formulation.
Ethanol 10.0% by weight
Glycerin 5.0
Lactitol 10.0
Maltitol 10.0
Fragrance 0.05
Coloring 0.001
Water remaining
100.0

(Example 6)
A lozenge was produced according to the following formulation.
Maltitol 72.3 wt%
Xylitol 20.0
Gum arabic 6.0
Fragrance 1.0
Sodium monofluorophosphate 0.7
100.0

(Example 7)
Chewing gum was manufactured according to the following formulation.
Gum base 20.0% by weight
Maltitol 45.0
Lactitol 33.0
Fragrance 2.0
100.0

(Example 8)
Candy was manufactured according to the following prescription.
Maltitol 50.0% by weight
Reduced water candy 34.0
Citric acid 2.0
Fragrance 0.2
Water remaining
100.0

Example 9
Tablet confectionery was produced according to the following prescription.
Maltitol 76.1% by weight
Lactitol 19.0
Sucrose fatty acid ester 0.2
Fragrance 0.2
Water 4.5
100.0

(Example 10)
Gummy jelly was manufactured according to the following prescription.
Gelatin 60.0% by weight
Reduced water candy 21.40
Maltitol 11.5
Vegetable oil 4.5
Malic acid 2.0
Fragrance 0.5
100.0

(Example 11)
A beverage was produced according to the following formulation.
Orange juice 30.0% by weight
Lactitol 15.0
Citric acid 0.1
Vitamin C 0.04
Fragrance 0.1
Water remaining
100.0

The sugar alcohol according to the present invention is a widely used material, and is useful for various products using the sugar alcohol, in particular, foods for preventing periodontal diseases.

This application claims priority from Japanese Patent Application No. 2009-165554 filed on Jul. 14, 2009, the contents of which are incorporated herein by reference.

Claims

An antibacterial agent for periodontal pathogen growth inhibition containing sugar alcohol as an active ingredient.
The antibacterial agent according to claim 1, wherein the sugar alcohol is lactitol or maltitol.
A mouthwash comprising the antibacterial agent according to claim 1 or 2.
A toothpaste comprising the antibacterial agent according to claim 1 or 2.
An inhalant comprising the antibacterial agent according to claim 1 or 2.
A lozenge comprising the antibacterial agent according to claim 1 or 2.
Food containing the antibacterial agent according to claim 1 or 2.