WO2010134650A1 - Methods for preparing a fermented ginseng concentrate or powder - Google Patents

Methods for preparing a fermented ginseng concentrate or powder Download PDF

Info

Publication number
WO2010134650A1
WO2010134650A1 PCT/KR2009/002635 KR2009002635W WO2010134650A1 WO 2010134650 A1 WO2010134650 A1 WO 2010134650A1 KR 2009002635 W KR2009002635 W KR 2009002635W WO 2010134650 A1 WO2010134650 A1 WO 2010134650A1
Authority
WO
WIPO (PCT)
Prior art keywords
ginseng
fermented
pectinase
galactosidase
beta
Prior art date
Application number
PCT/KR2009/002635
Other languages
French (fr)
Inventor
Dae Keun Sim
Jong Hwan Sung
Seung Kwon Lee
Min Goo Cho
Original Assignee
Il Hwa Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Il Hwa Co., Ltd. filed Critical Il Hwa Co., Ltd.
Priority to EP09844959.8A priority Critical patent/EP2432891B1/en
Priority to PCT/KR2009/002635 priority patent/WO2010134650A1/en
Priority to JP2012511737A priority patent/JP6170674B2/en
Priority to CN200980160042.9A priority patent/CN102459623B/en
Publication of WO2010134650A1 publication Critical patent/WO2010134650A1/en

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine

Definitions

  • the present invention relates to methods for preparing a fermented ginseng concentrate or powder.
  • Panax ginseng C.A. Meyer is one of the medicinal plants that have long been used for treating various diseases in Asian countries, including China, Korea, and Japan.
  • ginsenosides ginseng saponin
  • the main active ingredient of Panax ginseng are known to have various physiological activities, such as anti-aging activity, anti-inflammatory activity, antioxidant activities in the central nervous system, cardiovascular system, and the immune system (Wu et al., J. Immunol . , 148:1519-25, 1992; Lee, Facts about Ginseng , the Elixir of Life ., Hollyn International. New Jersey, 1992; Huang, The Pharmacology of Chinese Herbs . CRC Press.
  • ginsenosides are metabolized by bacteria inside the human intestine after intake, where their metabolites are known to have various physiological activities (Karikura et al., Chem . Pharm. Bull ., 39:2357-61, 1991; Kanaoda et al., J. Tradit . Med . 11:241-5, 1994; Akao et al., Biol . Pharm. Bull ., 21:245-9, 1998).
  • Rb1, Rb2, and Rc which are protopanaxadiol-type ginsenosides, are metabolized by human intestinal bacteria into IH-901 (20-O- ⁇ -D-glucopyranosyl-20(S)-protopandaxadiol) (Hasegawa et al., Planta Medica 63:463-40, 1997; Tawab et al., Drug Metab .
  • IH-901 is generally known for its anti-diabetic (Choi et al., J. Ginseng Res . 31(2): 79-85, 2007) and immune-enhancing activities. Further, IH-901 is known to induce an anti-metastasis or anti-cancer effect by blocking tumor invasion or preventing chromosomal mutation and tumor formation (Wakabayashi et al., Oncol . Res ., 9:411-7, 1998; Lee et al., Cancer Lett ., 144:39-43, 1999).
  • the present invention is directed to overcoming these deficiencies in the art.
  • the present invention relates to a method for preparing a fermented ginseng concentrate.
  • the method first involves subjecting ginseng to an extraction with a solvent to obtain a ginseng extract.
  • pectinase and beta-galactosidase are added to the ginseng extract under conditions effective to ferment the ginseng extract.
  • the fermented ginseng extract is concentrated to produce a fermented ginseng concentrate.
  • the present invention also relates to a method for preparing a fermented ginseng concentrate.
  • the method first involves suspending ginseng in a first solvent to obtain a ginseng solution.
  • pectinase and beta-galactosidase are added to the ginseng solution under conditions effective to ferment the ginseng solution.
  • the fermented ginseng solution is subjected to an extraction with a second solvent to obtain a fermented ginseng extract.
  • the fermented ginseng extract is concentrated to produce a fermented ginseng concentrate.
  • Another aspect of the present invention relates to fermented ginseng concentrates and fermented ginseng powder prepared by the above methods, as well as food compositions or functional food compositions containing such fermented ginseng concentrates and powder.
  • the methods for preparing a fermented ginseng concentrate and fermented ginseng powder according to the present invention involve simple steps and are capable of producing a fermented ginseng concentrate or powder having a high IH-901 content. Due to their high IH-901 content, the fermented ginseng concentrate or powder prepared according to the methods of the present invention can be effectively used in functional food compositions.
  • Fig. 1 is a graph depicting the results from a high-pressure liquid chromatography analysis of the fermented ginseng concentrate of the present invention.
  • Fig. 2 is a graph showing the average plasma concentrations of IH-901 over time for the subjects who took the fermented ginseng concentrate of the present invention.
  • Fig. 3 is a graph showing the maximum plasma concentrations (C max ) of IH-901 of each subject.
  • Fig. 4 is a graph showing the times to reach maximum plasma concentration (T max ) of IH-901 of each subject.
  • Fig. 5 is a graph showing the area under the plasma concentration time curve (AUC) of each subject.
  • the present invention relates to a method for preparing a fermented ginseng concentrate.
  • the method first involves subjecting ginseng to an extraction with a solvent to obtain a ginseng extract.
  • ginseng may include, but are not limited to, ginseng tail, white ginseng, fresh ginseng, dried ginseng, red ginseng, taekuk ginseng, Korean ginseng, Chinese ginseng, Japanese ginseng, Asian ginseng, American ginseng, extracts thereof, powders thereof, and mixtures thereof.
  • the solvent used in the extraction of ginseng may be any solvent, such as an aqueous solvent (e.g., water), an organic solvent, or any mixture thereof, as long as it does not affect the activity of the enzymes used in the method.
  • the organic solvent may include, without limitation, acetonitrile, dioxane, dimethyl sulfoxide, methanol, ethanol, 1-propanol, 2-propanol, and mixtures thereof.
  • the ginseng may be subjected to an extraction with the above solvents by using any commonly known solvent extraction methods.
  • an appropriate mixture of water and organic solvents may be used for the extraction, where the organic solvent in the mixture may increase the solubility of the ingredients in ginseng that are insoluble in water and/or the intermediates produced from the enzymatic reactions, resulting in an overall increase in the IH-901 content in the final product, i.e., the fermented ginseng concentrate or powder.
  • pectinase and beta-galactosidase are added to the ginseng extract under conditions effective to ferment the ginseng extract.
  • the ginseng extract may be diluted with a solvent before the pectinase and the beta-galactosidase are added.
  • the pectinase and the beta-galactosidase are added to the ginseng extract together or at the same time.
  • the pectinase and the beta-galactosidase are added to the ginseng extract separately or at different times.
  • the pectinase and the beta-galactosidase may be added to the ginseng extract in a relative ratio ranging from about 100:1 to about 1:100, from about 50:1 to about 1:50, from about 20:1 to about 1:20, from about 10:1 to about 1:10, from about 5:1 to about 1:5, from about 3:1 to about 1:3, or from about 2:1 to about 1:2.
  • the pectinase and the beta-galactosidase may be added to the ginseng extract in a relative ratio of about 100:1, about 50:1, about 20:1, about 10:1, about 5:1, about 3:1, about 2:1, about 1:2, about 1:3, about 1:5, about 1:10, about 1:20, about 1:50, or about 1:100.
  • the total amount of the pectinase and beta-galactosidase added to the ginseng extract may range from about 0.01 wt.% to about 50 wt.%, from about 0.05 wt.% to about 50 wt.%, from about 0.1 wt.% to about 50 wt.%, from about 0.5 wt.% to about 50 wt.%, from about 1 wt.% to about 50 wt.%, from about 2 wt.% to about 50 wt.%, from about 5 wt.% to about 50 wt.%, from about 10 wt.% to about 50 wt.%, from about 25 wt.% to about 50 wt.%, from about 0.01 wt.% to about 0.05 wt.%, from about 0.01 wt.% to about 0.1 wt.%, from about 0.01 wt.% to about 0.5 wt.%, from about 0.01
  • the total amount of the pectinase and beta-galactosidase added to the ginseng extract may be about 0.01 wt.%, about 0.05 wt.%, about 0.1 wt.%, about 0.5 wt.%, about 1 wt.%, about 2 wt.%, about 5 wt.%, about 10 wt.%, about 25 wt.%, or about 50 wt.%, based on the total weight of the ginseng extract or ginseng.
  • the pectinase and the beta-galactosidase are added to the ginseng extract where the reaction is carried out at a pH of from about 3 to about 8, from about 4 to about 8, from about 4.5 to about 8, from about 5 to about 8, from about 6 to about 8, from about 3 to about 4, from about 3 to about 4.5, from about 3 to about 5, from about 3 to about 6, from about 4 to about 4.5, from about 4.5 to about 5, or from about 5 to about 6.
  • the reaction is carried out at a pH of about 3, about 4, about 4.5, about 5, about 6, or about 8.
  • the reaction may be carried out at any pH as long as the activities of the pectinase and the beta-galactosidase are maintained.
  • the pH may be adjusted by adding to the ginseng extract solutions, such as but not limited to, a phosphate solution, citric acid solution, and sodium citrate buffer solution.
  • the reaction temperature for adding the pectinase and the beta-galactosidase to the ginseng extract may be any temperature as long as the activities of the pectinase and beta-galactosidase are maintained.
  • the reaction temperature range may range from about 10°C to about 70°C, from about 30°C to about 70°C, from about 40°C to about 70°C, from about 50°C to about 70°C, from about 60°C to about 70°C, from about 10°C to about 30°C, from about 10°C to about 40°C, from about 10°C to about 50°C, from about 10°C to about 60°C, from about 30°C to about 40°C, from about 40°C to about 50°C, or from about 50°C to about 60°C.
  • the reaction temperature may be about 10°C, about 30°C, about 40°C, about 50°C, about 60°C, or about 70°C.
  • reaction time may be any length of time, as long as the activities of the pectinase and beta-galactosidase are maintained.
  • the reaction time may range from about 1 hour to about 72 hours, from about 12 hours to about 72 hours, from about 24 hours to about 72 hours, from about 48 hours to about 72 hours, from about 60 hours to about 72 hours, from about 1 hour to about 12 hours, from about 1 hour to about 24 hours, from about 1 hour to about 48 hours, from about 1 hour to about 60 hours, from about 12 hours to about 24 hours, from about 24 to about 48 hours, or from about 48 to about 60 hours.
  • the reaction time may be about 1 hour, about 12 hours, about 24 hours, about 48 hours, about 60 hours, or about 72 hours.
  • the fermented ginseng extract is concentrated to produce a fermented ginseng concentrate.
  • the concentrating of the fermented ginseng extract may involve initially carrying out a centrifugation of the fermented ginseng extract and then concentrating only the separated supernatant.
  • the concentrating of the fermented ginseng extract may involve concentrating the fermented ginseng extract without any pre-processing steps.
  • the fermented ginseng extract may be concentrated by using commonly used devices, such as but not limited to, a vacuum decompression concentrator.
  • the method of the present invention may further include drying the prepared fermented ginseng concentrate (which may be in a liquid form) to produce fermented ginseng powder.
  • the drying may be carried out by any conventional drying method, such as but not limited to, drying under reduced pressure, hot-air drying, spray drying, fluidized bed drying, fluidized bed granulation, and freeze drying or lyophilization.
  • the present invention also relates to a method for preparing a fermented ginseng concentrate.
  • the method first involves suspending ginseng in a first solvent to obtain a ginseng solution.
  • pectinase and beta-galactosidase are added to the ginseng solution under conditions effective to ferment the ginseng solution.
  • the fermented ginseng solution is subjected to an extraction with a second solvent to obtain a fermented ginseng extract.
  • the first and second solvents may be an aqueous solvent (e.g., water), an organic solvent, or a mixture thereof.
  • the fermented ginseng extract is concentrated to produce a fermented ginseng concentrate. Descriptions regarding the various conditions used in this method, for example, with respect to addition of the pectinase and beta-galactosidase, extraction, and concentration already described above are not necessarily repeated herein.
  • Another aspect of the present invention relates to fermented ginseng concentrates and fermented ginseng powder prepared by the above methods.
  • the above methods of the present invention involve directly adding pectinase and beta-galactosidase to a ginseng extract or ginseng solution under conditions effective to ferment the ginseng extract or solution and thus are simple methods for preparing fermented ginseng concentrates or powder having a high IH-901 content. Since the fermented ginseng concentrate and powder prepared by the above methods of the present invention have a high IH-901 content, they can be effectively used as functional food compositions.
  • Another aspect of the present invention relates to food compositions containing the above fermented ginseng concentrate or powder, as well as functional food compositions containing a therapeutically effective amount of the above fermented ginseng concentrate or powder.
  • the fermented ginseng concentrate and powder of the present invention may contain a large amount of IH-901, which is known to possess immune-enhancing, anti-diabetic, anticancer, anti-aging, and anti-oxidation activities.
  • IH-901 which is known to possess immune-enhancing, anti-diabetic, anticancer, anti-aging, and anti-oxidation activities.
  • a person skilled in the art can properly determine the amount of the fermented ginseng concentrate or powder of the present invention to be added, depending on the purpose for using the functional food composition.
  • the fermented ginseng concentrate or powder of the present invention may be added to the functional food composition in an amount ranging from about 0.1 wt% to about 100 wt%, based on the total weight of the functional food composition.
  • the amount of the fermented ginseng concentrate or powder of the present invention added to the functional food composition may be lower than the above range. Since there are no safety issues, however, the fermented ginseng concentrate or powder of the present invention may be used in a very large amount.
  • the food compositions or functional food compositions of the present invention may be prepared by adding the fermented ginseng concentrate or powder of the present invention or mixtures thereof without any pre-processing.
  • the fermented ginseng concentrate or powder of the present invention may be initially processed by commonly known methods and other food components may then be added.
  • the food components that may be added to the food composition or functional food composition of the present invention may differ depending on the type of the food composition or functional food composition, and are not limited as long as they do not cause any side effects when mixed with the fermented ginseng concentrate or powder of the present invention.
  • the food compositions or functional food compositions of the present invention may be in a form suitable for oral consumption, for example, as soft or hard capsules, tablets, lozenges, dispersible powders or granules, emulsions, suspensions, and tea bags composed of dried powder made by commonly known methods.
  • the functional food composition may be added to various foods, such as but not limited to, beverages, gum, candies, snacks, vitamin complexes, health functional foods, etc., to provide immune-enhancing, anti-diabetic, anti-cancer, anti-oxidation, and/or anti-aging effects.
  • ginseng tail and 5 g of white ginseng were extracted with a mixture of water and ethanol (mixing ratio of 1:1; in an amount corresponding to 500% compared to the raw materials) four times at 80°C at 3-hour intervals to obtain 7 g of ginseng extract.
  • 100 ml of water and then 0.3 g of pectinase and 1 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50°C, pH 4-5, for 48 hours.
  • the reactant was filtered and concentrated under reduced pressure at 80°C to give 6.3 g of fermented ginseng concentrate according to the present invention.
  • ginseng extract 7 g was prepared as described in Example ⁇ 1-1> above. 100 ml of water and then 1 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50°C for 24 hours, and then the enzyme was inactivated by subjecting the mixture at a temperature of 80°C for 3 hours. The reactant was cooled and the pH was adjusted to 4-5 with citric acid. Then, 0.3 g of pectinase was added thereto where the mixture was subjected to a reaction for 24 hours. Subsequently, the reaction mixture was filtered and concentrated under reduced pressure at 80°C to give 5.9 g of fermented ginseng concentrate according to the present invention.
  • ginseng extract 5 g of ginseng tail and 5 g of white ginseng were extracted with a mixture of water and ethanol (mixing ratio of 1:1; in an amount corresponding to 500% compared to the raw materials) to obtain 7 g of ginseng extract. 100 ml of water and then 0.5 g of pectinase and 0.7 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50°C, pH 4-5, for 48 hours. The reactant was filtered and concentrated under reduced pressure at 80°C to give 6.2 g of fermented ginseng concentrate according to the present invention.
  • ginseng extract 5 g of ginseng tail and 5 g of white ginseng were extracted with a mixture of water and ethanol (mixing ratio of 1:1; in an amount corresponding to 500% compared to the raw materials) to obtain 7 g of ginseng extract. 100 ml of water and then 0.9 g of pectinase and 0.6 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50°C, pH 4-5, for 48 hours. The reactant was filtered and concentrated under reduced pressure at 80°C to give 5.9 g of fermented ginseng concentrate according to the present invention.
  • ginseng extract 5 g of ginseng tail and 5 g of white ginseng were extracted with a mixture of water and ethanol (mixing ratio of 1:1; in an amount corresponding to 500% compared to the raw materials) to obtain 7 g of ginseng extract. 100 ml of water and then 1.2 g of pectinase and 0.2 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50°C, pH 4-5, for 48 hours. The reactant was filtered and concentrated under reduced pressure at 80°C to give 6.3 g of fermented ginseng concentrate according to the present invention.
  • ginseng tail was extracted with a mixture of water and ethanol by the same method as described in Example ⁇ 1-1> above to obtain 7.2 g of ginseng extract.
  • the pH of the extract was adjusted to 3-4 with citric acid.
  • 0.3 g of pectinase and 1 g of beta-galactosidase were added to the ginseng extract, where the mixture was filtered and concentrated by the same method as described in Example ⁇ 1-1> above to obtain 6.8 g of fermented ginseng concentrate (II) according to the present invention.
  • ginseng powder 7 g was suspended in water. The pH was adjusted to 3-4.5 with citric acid. The suspended ginseng powder was reacted with 0.3 g of pectinase and 1 g of beta-galactosidase and extracted by the same method as described in Example ⁇ 1-11> above, and then filtered and concentrated to obtain 3.7 g of fermented ginseng concentrate according to the present invention.
  • the fermented ginseng concentrate according to the present invention in contrast to the ginseng extract, contains a large amount of IH-901. Furthermore, the IH-901 content in the fermented ginseng concentrate according to the present invention is 3.6 times higher than that in the fermented ginseng concentrate prepared using beta-galactosidase only and 1.5 times higher than that in the fermented ginseng concentrate prepared using pectinase only.
  • the C max , AUC, and T max of IH-901 in the fermented ginseng concentrate are about 24 times higher, 15 times higher, and 3.7 times lower than those of the ginseng concentrate, respectively.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Diabetes (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Medical Informatics (AREA)
  • Toxicology (AREA)
  • Epidemiology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Fruits And Vegetables (AREA)

Abstract

The present invention relates to a method for preparing a fermented ginseng concentrate. The method first involves subjecting ginseng to an extraction with a solvent to obtain a ginseng extract. Next, pectinase and beta-galactosidase are added to the ginseng extract under conditions effective to ferment the ginseng extract. The fermented extract is then concentrated to produce a fermented ginseng concentrate. The method may further involve drying the fermented ginseng concentrate to obtain fermented ginseng powder.

Description

METHODS FOR PREPARING A FERMENTED GINSENG CONCENTRATE OR POWDER
The present invention relates to methods for preparing a fermented ginseng concentrate or powder.
Background of the Invention
Panax ginseng C.A. Meyer (Araliaceae), is one of the medicinal plants that have long been used for treating various diseases in Asian countries, including China, Korea, and Japan. In particular, ginsenosides (ginseng saponin), the main active ingredient of Panax ginseng, are known to have various physiological activities, such as anti-aging activity, anti-inflammatory activity, antioxidant activities in the central nervous system, cardiovascular system, and the immune system (Wu et al., J. Immunol ., 148:1519-25, 1992; Lee, Facts about Ginseng , the Elixir of Life., Hollyn International. New Jersey, 1992; Huang, The Pharmacology of Chinese Herbs. CRC Press. Florida, 1999), anti-diabetic activity (Chang, Pharmacology and Application of Chinese Material Medica . Vol.1, World Scientific. Singapore, 1986), and anti-tumor activity (Sato et al., Biol . Pharm . Bull. 17:635-9, 1994; Mochizuki et al., Biol . Pharm . Bull. 18:1197-1202, 1995).
Further, ginsenosides are metabolized by bacteria inside the human intestine after intake, where their metabolites are known to have various physiological activities (Karikura et al., Chem . Pharm. Bull., 39:2357-61, 1991; Kanaoda et al., J. Tradit . Med . 11:241-5, 1994; Akao et al., Biol . Pharm. Bull., 21:245-9, 1998). For example, Rb1, Rb2, and Rc, which are protopanaxadiol-type ginsenosides, are metabolized by human intestinal bacteria into IH-901 (20-O-β-D-glucopyranosyl-20(S)-protopandaxadiol) (Hasegawa et al., Planta Medica 63:463-40, 1997; Tawab et al., Drug Metab . Dispos., 31:1065-71, 2003), while Re and Rg1, protopanaxatriol-type ginsenosides, are metabolized into ginsenoside Rh1 or ginsenoside F1 (Hasegawa et al., Planta Medica 63:463-40, 1997; Tawab et al., Drug Metab . Dispos., 31:1065-71, 2003), where the metabolites IH-901, Rh1, and F1 exhibit various physiological activities.
Specifically, IH-901 is generally known for its anti-diabetic (Choi et al., J. Ginseng Res. 31(2): 79-85, 2007) and immune-enhancing activities. Further, IH-901 is known to induce an anti-metastasis or anti-cancer effect by blocking tumor invasion or preventing chromosomal mutation and tumor formation (Wakabayashi et al., Oncol . Res., 9:411-7, 1998; Lee et al., Cancer Lett., 144:39-43, 1999).
Several researchers have tried to produce genuine prosapogenin or sapogenin by using methods, such as chemical synthesis, subacid hydrolysis, and alkali digestion (Han et al., Planta Medica 44:146-9, 1982; Chen et al., Chem . Pharm . Bull . 35:1653-5, 1987; Elyakov et al., Synthesis of the Ginseng Glycosides and Their Analogs. Proc . 6 th Int . Ginseng Symp . Seoul 74-83, 1993). However, the above methods are known to cause various side reactions, such as epimerization, hydration, and hydroxylation.
Thus, a number of methods that convert ginsenosides under mild conditions by utilizing enzymes (Ko et al., Biosci . Biotechnol . Biochem. 64:2739-43, 2000; Ko et al., Planta Med. 69:285-6, 2003) or intestinal bacteria (Hasegawa et al., Planta Medica 63:463-40, 1997; Bae et al., J. Microbial . Biotechnol. 13:9-14, 2003) have been studied. Specifically, methods for preparing IH-901 by hydrolyzing diol-type ginsenosides with naringinase, as well as methods for preparing IH-901 by administrating the diol-type ginsenosides orally to a rat and then having them digested in the colon have been developed. However, the above preparation methods were found to have certain drawbacks in that the production yield of IH-901 is extremely low and various secondary metabolites are produced, making it difficult to obtain highly pure IH-901 (Karikura et al., Chem . Pharm . Bull. 38:2859, 1990).
More recently, other methods for preparing IH-901 by reacting a variety of enzymes with ginsenosides (Korean Laid-open Patent Publication No. 2003-94757; Korean Patent Nos. 418604 and 377546) have been studied, but the amount of IH-901 produced using these methods was found to be too low to have any significant effect. Further, the above methods involve complicated steps and are unsuitable for preparing a fermented ginseng concentrate having a high content of IH-901.
The present invention is directed to overcoming these deficiencies in the art.
Summary of the Invention
The present invention relates to a method for preparing a fermented ginseng concentrate. The method first involves subjecting ginseng to an extraction with a solvent to obtain a ginseng extract. Next, pectinase and beta-galactosidase are added to the ginseng extract under conditions effective to ferment the ginseng extract. Then, the fermented ginseng extract is concentrated to produce a fermented ginseng concentrate.
The present invention also relates to a method for preparing a fermented ginseng concentrate. The method first involves suspending ginseng in a first solvent to obtain a ginseng solution. Next, pectinase and beta-galactosidase are added to the ginseng solution under conditions effective to ferment the ginseng solution. Then, the fermented ginseng solution is subjected to an extraction with a second solvent to obtain a fermented ginseng extract. Finally, the fermented ginseng extract is concentrated to produce a fermented ginseng concentrate.
Another aspect of the present invention relates to fermented ginseng concentrates and fermented ginseng powder prepared by the above methods, as well as food compositions or functional food compositions containing such fermented ginseng concentrates and powder.
The methods for preparing a fermented ginseng concentrate and fermented ginseng powder according to the present invention involve simple steps and are capable of producing a fermented ginseng concentrate or powder having a high IH-901 content. Due to their high IH-901 content, the fermented ginseng concentrate or powder prepared according to the methods of the present invention can be effectively used in functional food compositions.
Brief Description of the Drawings
Fig. 1 is a graph depicting the results from a high-pressure liquid chromatography analysis of the fermented ginseng concentrate of the present invention.
Fig. 2 is a graph showing the average plasma concentrations of IH-901 over time for the subjects who took the fermented ginseng concentrate of the present invention.
Fig. 3 is a graph showing the maximum plasma concentrations (Cmax) of IH-901 of each subject.
Fig. 4 is a graph showing the times to reach maximum plasma concentration (Tmax) of IH-901 of each subject.
Fig. 5 is a graph showing the area under the plasma concentration time curve (AUC) of each subject.
Detailed Description of the Invention
The present invention relates to a method for preparing a fermented ginseng concentrate.
The method first involves subjecting ginseng to an extraction with a solvent to obtain a ginseng extract. Examples of ginseng may include, but are not limited to, ginseng tail, white ginseng, fresh ginseng, dried ginseng, red ginseng, taekuk ginseng, Korean ginseng, Chinese ginseng, Japanese ginseng, Asian ginseng, American ginseng, extracts thereof, powders thereof, and mixtures thereof.
The solvent used in the extraction of ginseng may be any solvent, such as an aqueous solvent (e.g., water), an organic solvent, or any mixture thereof, as long as it does not affect the activity of the enzymes used in the method. In some embodiments, the organic solvent may include, without limitation, acetonitrile, dioxane, dimethyl sulfoxide, methanol, ethanol, 1-propanol, 2-propanol, and mixtures thereof.
In some embodiments, the ginseng may be subjected to an extraction with the above solvents by using any commonly known solvent extraction methods. In certain embodiments, an appropriate mixture of water and organic solvents may be used for the extraction, where the organic solvent in the mixture may increase the solubility of the ingredients in ginseng that are insoluble in water and/or the intermediates produced from the enzymatic reactions, resulting in an overall increase in the IH-901 content in the final product, i.e., the fermented ginseng concentrate or powder.
Next, pectinase and beta-galactosidase are added to the ginseng extract under conditions effective to ferment the ginseng extract. In some embodiments, the ginseng extract may be diluted with a solvent before the pectinase and the beta-galactosidase are added. In some embodiments, the pectinase and the beta-galactosidase are added to the ginseng extract together or at the same time. In other embodiments, the pectinase and the beta-galactosidase are added to the ginseng extract separately or at different times.
In some embodiments, the pectinase and the beta-galactosidase may be added to the ginseng extract in a relative ratio ranging from about 100:1 to about 1:100, from about 50:1 to about 1:50, from about 20:1 to about 1:20, from about 10:1 to about 1:10, from about 5:1 to about 1:5, from about 3:1 to about 1:3, or from about 2:1 to about 1:2. In other embodiments, the pectinase and the beta-galactosidase may be added to the ginseng extract in a relative ratio of about 100:1, about 50:1, about 20:1, about 10:1, about 5:1, about 3:1, about 2:1, about 1:2, about 1:3, about 1:5, about 1:10, about 1:20, about 1:50, or about 1:100.
In some embodiments, the total amount of the pectinase and beta-galactosidase added to the ginseng extract may range from about 0.01 wt.% to about 50　wt.%, from about 0.05 wt.% to about 50　wt.%, from about 0.1 wt.% to about 50　wt.%, from about 0.5 wt.% to about 50　wt.%, from about 1 wt.% to about 50　wt.%, from about 2 wt.% to about 50　wt.%, from about 5 wt.% to about 50　wt.%, from about 10 wt.% to about 50　wt.%, from about 25 wt.% to about 50　wt.%, from about 0.01 wt.% to about 0.05　wt.%, from about 0.01 wt.% to about 0.1　wt.%, from about 0.01 wt.% to about 0.5　wt.%, from about 0.01 wt.% to about 1　wt.%, from about 0.01 wt.% to about 2　wt.%, from about 0.01 wt.% to about 5　wt.%, from about 0.01 wt.% to about 10　wt.%, from about 0.01 wt.% to about 25　wt.%, from about 0.05 wt.% to about 0.1　wt.%, from about 0.1 wt.% to about 0.5　wt.%, from about 0.5 wt.% to about 1　wt.%, from about 1 wt.% to about 2　wt.%, from about 2 wt.% to about 5　wt.%, from about 5 wt.% to about 10　wt.%, or from about 10 wt.% to about 25　wt.%, based on the total weight of the ginseng extract or ginseng. In other embodiments, the total amount of the pectinase and beta-galactosidase added to the ginseng extract may be about 0.01 wt.%, about 0.05 wt.%, about 0.1 wt.%, about 0.5 wt.%, about 1 wt.%, about 2 wt.%, about 5 wt.%, about 10 wt.%, about 25 wt.%, or about 50 wt.%, based on the total weight of the ginseng extract or ginseng.
In some embodiments, the pectinase and the beta-galactosidase are added to the ginseng extract where the reaction is carried out at a pH of from about 3 to about 8, from about 4 to about 8, from about 4.5 to about 8, from about 5 to about 8, from about 6 to about 8, from about 3 to about 4, from about 3 to about 4.5, from about 3 to about 5, from about 3 to about 6, from about 4 to about 4.5, from about 4.5 to about 5, or from about 5 to about 6. In other embodiments, the reaction is carried out at a pH of about 3, about 4, about 4.5, about 5, about 6, or about 8. The reaction, however, may be carried out at any pH as long as the activities of the pectinase and the beta-galactosidase are maintained. The pH may be adjusted by adding to the ginseng extract solutions, such as but not limited to, a phosphate solution, citric acid solution, and sodium citrate buffer solution.
The reaction temperature for adding the pectinase and the beta-galactosidase to the ginseng extract may be any temperature as long as the activities of the pectinase and beta-galactosidase are maintained. In some embodiments, the reaction temperature range may range from about 10℃ to about 70℃, from about 30℃ to about 70℃, from about 40℃ to about 70℃, from about 50℃ to about 70℃, from about 60℃ to about 70℃, from about 10℃ to about 30℃, from about 10℃ to about 40℃, from about 10℃ to about 50℃, from about 10℃ to about 60℃, from about 30℃ to about 40℃, from about 40℃ to about 50℃, or from about 50℃ to about 60℃. In other embodiments, the reaction temperature may be about 10℃, about 30℃, about 40℃, about 50℃, about 60℃, or about 70℃.
Further, the reaction time may be any length of time, as long as the activities of the pectinase and beta-galactosidase are maintained. In some embodiments, the reaction time may range from about 1 hour to about 72 hours, from about 12 hours to about 72 hours, from about 24 hours to about 72 hours, from about 48 hours to about 72 hours, from about 60 hours to about 72 hours, from about 1 hour to about 12 hours, from about 1 hour to about 24 hours, from about 1 hour to about 48 hours, from about 1 hour to about 60 hours, from about 12 hours to about 24 hours, from about 24 to about 48 hours, or from about 48 to about 60 hours. In other embodiments, the reaction time may be about 1 hour, about 12 hours, about 24 hours, about 48 hours, about 60 hours, or about 72 hours.
Finally, the fermented ginseng extract is concentrated to produce a fermented ginseng concentrate. In some embodiments, the concentrating of the fermented ginseng extract may involve initially carrying out a centrifugation of the fermented ginseng extract and then concentrating only the separated supernatant. In other embodiments, the concentrating of the fermented ginseng extract may involve concentrating the fermented ginseng extract without any pre-processing steps. The fermented ginseng extract may be concentrated by using commonly used devices, such as but not limited to, a vacuum decompression concentrator.
In some embodiments, the method of the present invention may further include drying the prepared fermented ginseng concentrate (which may be in a liquid form) to produce fermented ginseng powder. The drying may be carried out by any conventional drying method, such as but not limited to, drying under reduced pressure, hot-air drying, spray drying, fluidized bed drying, fluidized bed granulation, and freeze drying or lyophilization.
The present invention also relates to a method for preparing a fermented ginseng concentrate. The method first involves suspending ginseng in a first solvent to obtain a ginseng solution. Next, pectinase and beta-galactosidase are added to the ginseng solution under conditions effective to ferment the ginseng solution. Then, the fermented ginseng solution is subjected to an extraction with a second solvent to obtain a fermented ginseng extract. In some embodiments, the first and second solvents may be an aqueous solvent (e.g., water), an organic solvent, or a mixture thereof. Finally, the fermented ginseng extract is concentrated to produce a fermented ginseng concentrate. Descriptions regarding the various conditions used in this method, for example, with respect to addition of the pectinase and beta-galactosidase, extraction, and concentration already described above are not necessarily repeated herein.
Another aspect of the present invention relates to fermented ginseng concentrates and fermented ginseng powder prepared by the above methods. The above methods of the present invention involve directly adding pectinase and beta-galactosidase to a ginseng extract or ginseng solution under conditions effective to ferment the ginseng extract or solution and thus are simple methods for preparing fermented ginseng concentrates or powder having a high IH-901 content. Since the fermented ginseng concentrate and powder prepared by the above methods of the present invention have a high IH-901 content, they can be effectively used as functional food compositions.
Another aspect of the present invention relates to food compositions containing the above fermented ginseng concentrate or powder, as well as functional food compositions containing a therapeutically effective amount of the above fermented ginseng concentrate or powder.
The fermented ginseng concentrate and powder of the present invention may contain a large amount of IH-901, which is known to possess immune-enhancing, anti-diabetic, anticancer, anti-aging, and anti-oxidation activities. A person skilled in the art can properly determine the amount of the fermented ginseng concentrate or powder of the present invention to be added, depending on the purpose for using the functional food composition. Generally, the fermented ginseng concentrate or powder of the present invention may be added to the functional food composition in an amount ranging from about 0.1 wt% to about 100　wt%, based on the total weight of the functional food composition. In certain embodiments, such as when taking the functional food composition on a long term basis for health management, the amount of the fermented ginseng concentrate or powder of the present invention added to the functional food composition may be lower than the above range. Since there are no safety issues, however, the fermented ginseng concentrate or powder of the present invention may be used in a very large amount.
In some embodiments, the food compositions or functional food compositions of the present invention may be prepared by adding the fermented ginseng concentrate or powder of the present invention or mixtures thereof without any pre-processing. In other embodiments, the fermented ginseng concentrate or powder of the present invention may be initially processed by commonly known methods and other food components may then be added. The food components that may be added to the food composition or functional food composition of the present invention may differ depending on the type of the food composition or functional food composition, and are not limited as long as they do not cause any side effects when mixed with the fermented ginseng concentrate or powder of the present invention.
The food compositions or functional food compositions of the present invention may be in a form suitable for oral consumption, for example, as soft or hard capsules, tablets, lozenges, dispersible powders or granules, emulsions, suspensions, and tea bags composed of dried powder made by commonly known methods. In some embodiments, the functional food composition may be added to various foods, such as but not limited to, beverages, gum, candies, snacks, vitamin complexes, health functional foods, etc., to provide immune-enhancing, anti-diabetic, anti-cancer, anti-oxidation, and/or anti-aging effects.
Examples
The following examples are provided to illustrate embodiments of the present invention but are by no means intended to limit its scope.
Example 1 - Preparation of Fermented Ginseng Concentrate Using Pectinase and beta - Galactosidase
<1-1> Preparation of Fermented Ginseng Concentrate Using Ginseng Tail and White Ginseng
5 g of ginseng tail and 5 g of white ginseng were extracted with a mixture of water and ethanol (mixing ratio of 1:1; in an amount corresponding to 500% compared to the raw materials) four times at 80℃ at 3-hour intervals to obtain 7 g of ginseng extract. 100 ml of water and then 0.3 g of pectinase and 1 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50℃, pH 4-5, for 48 hours. The reactant was filtered and concentrated under reduced pressure at 80℃ to give 6.3 g of fermented ginseng concentrate according to the present invention.
<1-2> Preparation of Fermented Ginseng Concentrate Using Ginseng Tail and White Ginseng
7 g of ginseng extract was prepared as described in Example <1-1> above. 100 ml of water and then 1 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50℃ for 24 hours, and then the enzyme was inactivated by subjecting the mixture at a temperature of 80℃ for 3 hours. The reactant was cooled and the pH was adjusted to 4-5 with citric acid. Then, 0.3 g of pectinase was added thereto where the mixture was subjected to a reaction for 24 hours. Subsequently, the reaction mixture was filtered and concentrated under reduced pressure at 80℃ to give 5.9 g of fermented ginseng concentrate according to the present invention.
<1-3> Preparation of Fermented Ginseng Concentrate Using Ginseng Tail and White Ginseng
5 g of ginseng tail and 5 g of white ginseng were extracted with a mixture of water and ethanol (mixing ratio of 1:1; in an amount corresponding to 500% compared to the raw materials) to obtain 7 g of ginseng extract. 100 ml of water and then 0.5 g of pectinase and 0.7 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50℃, pH 4-5, for 48 hours. The reactant was filtered and concentrated under reduced pressure at 80℃ to give 6.2 g of fermented ginseng concentrate according to the present invention.
<1-4> Preparation of Fermented Ginseng Concentrate Using Ginseng Tail and White Ginseng
5 g of ginseng tail and 5 g of white ginseng were extracted with a mixture of water and ethanol (mixing ratio of 1:1; in an amount corresponding to 500% compared to the raw materials) to obtain 7 g of ginseng extract. 100 ml of water and then 0.9 g of pectinase and 0.6 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50℃, pH 4-5, for 48 hours. The reactant was filtered and concentrated under reduced pressure at 80℃ to give 5.9 g of fermented ginseng concentrate according to the present invention.
<1-5> Preparation of Fermented Ginseng Concentrate Using Ginseng Tail and White Ginseng
5 g of ginseng tail and 5 g of white ginseng were extracted with a mixture of water and ethanol (mixing ratio of 1:1; in an amount corresponding to 500% compared to the raw materials) to obtain 7 g of ginseng extract. 100 ml of water and then 1.2 g of pectinase and 0.2 g of beta-galactosidase were added to the ginseng extract, where the reaction mixture was maintained at 50℃, pH 4-5, for 48 hours. The reactant was filtered and concentrated under reduced pressure at 80℃ to give 6.3 g of fermented ginseng concentrate according to the present invention.
<1-6> Preparation of Fermented Ginseng Concentrate Using Ginseng Tail
10 g of ginseng tail was extracted with 100 ml of water to obtain 7 g of ginseng extract. The pH of the extract was adjusted to 3-4 with citric acid. 0.3　g of pectinase and 1　g of beta-galactosidase were added to the ginseng extract, where the mixture was subjected to a reaction at 50℃ for 48 hours. Subsequently, the reactant was filtered and concentrated by the same method as described in Example <1-1> above to obtain 6.5 g of fermented ginseng concentrate (I) according to the present invention.
Alternatively, 10 g of ginseng tail was extracted with a mixture of water and ethanol by the same method as described in Example <1-1> above to obtain 7.2 g of ginseng extract. The pH of the extract was adjusted to 3-4 with citric acid. 0.3 g of pectinase and 1 g of beta-galactosidase were added to the ginseng extract, where the mixture was filtered and concentrated by the same method as described in Example <1-1> above to obtain 6.8 g of fermented ginseng concentrate (II) according to the present invention.
<1-7> Preparation of Fermented Ginseng Concentrate Using Ginseng Tail
10 g of ginseng tail was extracted with 100 ml of water to obtain 7 g of ginseng extract. 100 ml of water and then 1 g of beta-galactosidase were added to the ginseng extract where the reaction mixture was maintained at 50℃ for 24 hours, and then the enzyme was inactivated by subjecting the mixture at a temperature of 80℃ for 3 hours. The reactant was cooled and the pH was adjusted to 4-5 with citric acid. Then, 0.3 g of pectinase was added thereto and reacted at 50℃ for 24 hours. Subsequently, the reactant was filtered and concentrated under reduced pressure at 80℃ to obtain 6.5 g of fermented ginseng concentrate according to the present invention.
<1-8> Preparation of Fermented Ginseng Concentrate Using White Ginseng
1 kg of white ginseng was extracted with 5ℓ of ethanol to obtain 540 g of ginseng extract. 7 g of the extract was concentrated and then suspended in water. 0.3 g of pectinase and 1 g of beta-galactosidase were added thereto, where the mixture was reacted, filtered, and concentrated by the same method as described in Example <1-1> above to obtain 5.2 g of fermented ginseng concentrate according to the present invention.
<1-9> Preparation of Fermented Ginseng Concentrate Using White Ginseng
1 kg of white ginseng was extracted with 5ℓ of ethanol to obtain 540 g of ginseng extract. 7 g of the extract was concentrated and then suspended in water. 0.5 g of pectinase and 0.5 g of beta-galactosidase were added thereto, where the mixture was reacted, filtered, and concentrated by the same method as described in Example <1-1> above to obtain 5.1 g of fermented ginseng concentrate according to the present invention.
<1-10> Preparation of Fermented Ginseng Concentrate Using White Ginseng
1 kg of white ginseng was extracted with 5ℓ of ethanol to obtain 540 g of ginseng extract. 7 g of the extract was concentrated and then suspended in water. 1.5 g of pectinase and 0.2 g of beta-galactosidase were added thereto, where the mixture was reacted, filtered, and concentrated by the same method as described in Example <1-1> above to obtain 6.2 g of fermented ginseng concentrate according to the present invention.
<1-11> Preparation of Fermented Ginseng Concentrate Using Fresh Ginseng
6 g of fresh ginseng was sliced into pieces and sterilized. After the ginseng pieces were suspended in water, the pH was adjusted to 3~4.5 with citric acid, and 0.3 g of pectinase and 1 g of beta-galactosidase were added thereto where the mixture was subjected to a reaction at 50℃ for 72 hours. The reactant was extracted with a mixture of water and ethanol prepared by the same method as described in Example <1-1> above four times at 80℃ for 4 hours. Then, the extract was filtered and concentrated to obtain 3.5 g of fermented ginseng concentrate according to the present invention.
<1-12> Preparation of Fermented Ginseng Concentrate Using Fresh Ginseng
6 g of fresh ginseng was sliced into pieces and sterilized. After the ginseng pieces were suspended in water, 1g of beta-galactosidase was added thereto where the mixture was subjected to a reaction at 50℃ for 24 hours and inactivated at 80℃ for 3 hours. The reactant was cooled and the pH was adjusted to 4-5 with citric acid. Then, 0.3 g of pectinase was added thereto and reacted at 50℃ for 24 hours. Subsequently, the reactant was filtered and then extracted with a mixture of water and ethanol prepared by the same method as described in Example <1-1> above four times at 80℃ for 4 hours. Then, the extract was concentrated under reduced pressure at 80℃ to obtain 2.9 g of fermented ginseng concentrate according to the present invention.
<1-13> Preparation of Fermented Ginseng Concentrate Using Fresh Ginseng
6 g of fresh ginseng was sliced into pieces and sterilized. After the ginseng pieces were suspended in water, the pH was adjusted to 3-4 with citric acid. Then, 0.7g of pectinase and 0.6g of beta-galactosidase were added thereto where the mixture was subjected to a reaction at 50℃ for 72 hours. Subsequently, the reactant was filtered and concentrated to obtain 2.9 g of fermented ginseng concentrate according to the present invention.
<1-14> Preparation of Fermented Ginseng Concentrate Using Fresh Ginseng
6 g of fresh ginseng was sliced into pieces and sterilized. After the ginseng pieces were suspended in water, 0.7g of beta-galactosidase was added thereto where the mixture was subjected to a reaction at 50℃ for 24 hours and inactivated at 80℃ for 3 hours. The reactant was cooled and the pH was adjusted to 4-5 with citric acid. Then, 0.6 g of pectinase was added thereto and reacted at 50℃ for 24 hours. Subsequently, the reactant was filtered and then extracted with a mixture of water and ethanol prepared by the same method as described in Example <1-1> above four times at 80℃ for 4 hours. Then, the extract was concentrated under reduced pressure at 80℃ to obtain 2.3 g of fermented ginseng concentrate according to the present invention.
<1-15> Preparation of Fermented Ginseng Concentrate Using Fresh Ginseng
6 g of fresh ginseng was sliced into pieces and sterilized. After the ginseng pieces were suspended in water, the pH was adjusted to 3-4.5 with citric acid. Then, 0.9g of pectinase and 0.5g of beta-galactosidase were added thereto where the mixture was subjected to a reaction at 50℃ for 72 hours. Subsequently, the reactant was filtered and concentrated to obtain 2.5 g of fermented ginseng concentrate according to the present invention.
<1-16> Preparation of Fermented Ginseng Concentrate Using Fresh Ginseng
6 g of fresh ginseng was sliced into pieces and sterilized. After the ginseng pieces were suspended in water, the pH was adjusted to 3-4.5 with citric acid. Then, 2 g of pectinase and 0.2 g of beta-galactosidase were added thereto where the mixture was subjected to a reaction at 50℃ for 72 hours. Subsequently, the reactant was filtered and concentrated to obtain 3.3 g of fermented ginseng concentrate according to the present invention.
<1-17> Preparation of Fermented Ginseng Concentrate Using Fresh Ginseng
6 g of fresh ginseng was sliced into pieces and sterilized. After the ginseng pieces were suspended in water, 0.5 g of beta-galactosidase was added thereto where the mixture was subjected to a reaction at 50℃ for 24 hours and inactivated at 80℃ for 3 hours. The reactant was cooled and the pH was adjusted to 4-5 with citric acid. Then, 1.5 g of pectinase was added thereto and reacted at 50℃ for 24 hours. Subsequently, the reactant was filtered and then extracted with a mixture of water and ethanol prepared by the same method as described in Example <1-1> above four times at 80℃ for 4 hours. Then, the extract was concentrated under reduced pressure at 80℃ to obtain 3.1 g of fermented ginseng concentrate according to the present invention.
<1-18> Preparation of Fermented Ginseng Concentrate Using Ginseng Powder
7 g of ginseng powder was suspended in water. The pH was adjusted to 3-4.5 with citric acid. The suspended ginseng powder was reacted with 0.3 g of pectinase and 1 g of beta-galactosidase and extracted by the same method as described in Example <1-11> above, and then filtered and concentrated to obtain 3.7 g of fermented ginseng concentrate according to the present invention.
Comparative Example 1 - Preparation of Fermented Ginseng Concentrate Using Pectinase Only
100 ml of water was added to 7 g of ginseng extract prepared by the same method as described in Example <1-1> above. Then, 1.3 g of pectinase was added to the extract, which was subsequently reacted and concentrated under reduced pressure by the same method as described in Example <1-1> above to obtain 5.6 g of fermented ginseng concentrate according to the present invention.
Comparative Example 2 - Preparation of Fermented Ginseng Concentrate Using beta-Galactosidase Only
100 ml of water was added to 7 g of ginseng extract prepared by the same method as described in Example <1-1> above. Then, 1.3 g of beta-galactosidase was added to the extract, which was subsequently reacted and concentrated under reduced pressure by the same method as described in Example　<1-1> above to obtain 5.4 g of fermented ginseng concentrate according to the present invention.
Experimental Example 1 - The IH -901 Content in the Fermented Ginseng Concentrate of the Present Invention
In order to analyze the content of IH-901 (20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol) in the fermented ginseng concentrate prepared by the method described in Example <1-1> above, 1 g of the obtained fermented ginseng concentrate was taken and extracted with 60 ml of water-saturated butanol four times. The butanol fraction was concentrated to obtain 120 mg of concentrate, and the concentrate was dissolved in methanol to analyze the IH-901 content by ultra performance liquid chromatography (UPLC). The results of the UPLC analysis are shown in Fig. 1. The IH-901 content in the ginseng extract prepared in Example <1-1> above was also measured in the same manner as above.
Further, the IH-901 content in the fermented ginseng concentrate prepared by the method described in Comparative Example 1 or Comparative Example 2 above was analyzed in the same manner as above.
The results of the above analyses are summarized in Table 1 below.
Table 1
Component Content of each component
Ginseng Extract of Example 1-1 Fermented Ginseng Concentrate of Example 1-1 Fermented Ginseng Concentrate of Comp. Example 1 Fermented Ginseng Concentrate of Comp. Example 2
Ginsenoside Rb1 17.6 mg/g 1.2 mg/g 6.3 mg/g 3.2 mg/g
Ginsenoside Rb2 14.6 mg/g 1.1 mg/g 7.1 mg/g 5.7 mg/g
Ginsenoside Rc 18.9 mg/g 0.1 mg/g 9.3 mg/g 6.3 mg/g
Ginsenoside Rd 7.9 mg/g 1.4 mg/g 2.2 mg/g 4.2 mg/g
IH-901 0 mg/g 36 mg/g 24 mg/g 10 mg/g
As shown in Table 1 above, the fermented ginseng concentrate according to the present invention, in contrast to the ginseng extract, contains a large amount of IH-901. Furthermore, the IH-901 content in the fermented ginseng concentrate according to the present invention is 3.6 times higher than that in the fermented ginseng concentrate prepared using beta-galactosidase only and 1.5 times higher than that in the fermented ginseng concentrate prepared using pectinase only.
Experimental Example 2 - Study on Body's Absorption of IH -901 of the Fermented Ginseng Concentrate of the Present Invention
Twenty four healthy male volunteers of ages ranging from 20 to 45, whose body weights are within 20% of the ideal body weights, were used as subjects for the study (randomized, 2 X 2 cross-over design).
Each volunteer received the fermented ginseng extract or ginseng extract for pharmacokinetic characteristic assessments. Blood samples were taken at 0, 0.5, 1, 1.5, 2, 3, 4, 5, 6, 7, 8, 10, 12, and 24 hours after administration of the fermented ginseng extract or ginseng extract and were analyzed by LC-MS/MS. The average plasma concentrations of IH-901 over time are shown in Fig. 2. The average maximum plasma concentration (Cmax), average time to reach maximum plasma concentration (Tmax), and area under the plasma concentration time curve (AUC) values measured are shown in Table 2 below, where Cmax is the average of the maximum concentration values of each subject irrespective of time and Tmax is the average of the time values corresponding to the Cmax values of each subject. The Cmax, Tmax, and AUC values for each subject are shown in Figs. 3 to 5.
Table 2
Cmax Tmax AUC
Fermented ginseng concentrate 325.00±91.97 ng/ml 3.29 hr 2083.09±524.68 ng/ml
Ginseng concentrate 13.88±7.24 ng/ml 12.04 hr 134.5±63.10 ng/ml
As shown in Table 2 above, the Cmax, AUC, and Tmax of IH-901 in the fermented ginseng concentrate are about 24 times higher, 15 times higher, and 3.7 times lower than those of the ginseng concentrate, respectively.
Therefore, it was found that when a ginseng concentrate was taken, each individual had different capabilities with respect to metabolizing ginsenosides to IH-901 and only a small amount of IH-901 was absorbed in the body. Further, when a ginseng concentrate was taken, each individual exhibited different average times in reaching the maximum plasma concentration.
In contrast, when a fermented ginseng concentrate was taken, the amount of IH-901 absorbed and the average time to reach maximum plasma level were constant for each individual. Further, when a fermented ginseng concentrate was taken, a large amount of IH-901 was absorbed in the body.
Accordingly, the above results demonstrate that a steady and constant effect (with little individual variation) can be expected when subjects take fermented ginseng concentrate.
Example 2 - Preparation of Fermented Ginseng Powder According to the Present Invention
<2-1> Preparation of Fermented Ginseng Powder Using Ginseng Tail and White Ginseng
100 g of water was added to 7 g of an extract of ginseng prepared by the same method as described in Example <1-1> above. Then, 0.3 g of pectinase and 1　g of beta-galactosidase were added to the extract, which was subsequently reacted at 50℃, pH 4-5, for 24 hours. The reacted extract was dried under reduced pressure at 80℃ to obtain 4.23 g of fermented ginseng powder according to the present invention.
<2-2> Preparation of Fermented Ginseng Powder Using Ginseng Powder
7 g of ginseng powder was suspended in water, and then the pH was adjusted to 3-4.5. Next, 0.3 g of pectinase and 1 g of beta-galactosidase were added to the suspension, which was subsequently reacted at 50℃ for 24 hours and extracted with a mixture of water and ethanol prepared by the same method as in described in Example <1-1> above four times at 80℃ for 4 hours. The extract was then dried under reduced pressure to obtain 2.6 g of fermented ginseng powder.
Although the invention has been described in detail for the purpose of illustration, it is understood that such detail is solely for that purpose, and variations can be made therein by those skilled in the art without departing from the spirit and scope of the invention which is defined by the following claims.

Claims (32)

  1. A method for preparing a fermented ginseng concentrate comprising:
    subjecting ginseng to an extraction with a solvent to obtain a ginseng extract;
    adding pectinase and beta-galactosidase to the ginseng extract under conditions effective to ferment the ginseng extract; and
    concentrating the fermented ginseng extract to produce a fermented ginseng concentrate.
  2. The method according to Claim 1, wherein the ginseng is one or more of ginseng tail, white ginseng, fresh ginseng, dried ginseng, red ginseng, taekuk ginseng, Korean ginseng, Chinese ginseng, Japanese ginseng, Asian ginseng, American ginseng, extracts thereof, powders thereof, and mixtures thereof.
  3. The method according to Claim 1, wherein the solvent is one or more of an aqueous solvent, organic solvent, and a mixture thereof.
  4. The method according to Claim 1, wherein said adding comprises adding the pectinase and the beta-galactosidase together.
  5. The method according to Claim 1, wherein said adding comprises adding the pectinase and the beta-galactosidase separately.
  6. The method according to Claim 1, wherein said adding comprises adding the pectinase and the beta-galactosidase in a relative ratio ranging from about 100:1 to about 1:100, wherein the amount of the pectinase and beta-galactosidase ranges from about 0.01 wt% to about 50 wt% based on the total amount of the ginseng extract or ginseng.
  7. The method according to Claim 1, wherein said adding comprises adding the pectinase and the beta-galactosidase to the ginseng extract at a temperature of from about 10℃ to about 70℃.
  8. The method according to Claim 1, wherein said adding comprises adding the pectinase and the beta-galactosidase to the ginseng extract for a time period from about 1 to about 72 hours.
  9. The method according to Claim 1 further comprising:
    drying the fermented ginseng concentrate after said concentrating to produce fermented ginseng powder.
  10. The method according to Claim 9, wherein said drying is carried out by drying under reduced pressure, hot-air drying, spray drying, fluidized bed drying, fluidized bed granulation, or freeze drying.
  11. A fermented ginseng concentrate prepared by the method according to Claim 1.
  12. A food composition comprising the fermented ginseng concentrate of Claim 11.
  13. A functional food composition comprising a therapeutically effective amount of the fermented ginseng concentrate of Claim 11.
  14. A fermented ginseng powder prepared by the method according to Claim　9.
  15. A food composition comprising the fermented ginseng powder of Claim　14.
  16. A functional food composition comprising a therapeutically effective amount of the fermented ginseng powder of Claim 14.
  17. A method for preparing a fermented ginseng concentrate comprising:
    suspending ginseng in a first solvent to obtain a ginseng solution;
    adding pectinase and beta-galactosidase to the ginseng solution under conditions effective to ferment the ginseng solution;
    subjecting the fermented ginseng solution to an extraction with a second solvent to obtain a fermented ginseng extract; and
    concentrating the fermented ginseng extract to produce a fermented ginseng concentrate.
  18. The method according to Claim 17, wherein the ginseng is one or more of ginseng tail, white ginseng, fresh ginseng, dried ginseng, red ginseng, taekuk ginseng, Korean ginseng, Chinese ginseng, Japanese ginseng, Asian ginseng, American ginseng, extracts thereof, powders thereof, and mixtures thereof.
  19. The method according to Claim 17, wherein said adding comprises adding the pectinase and the beta-galactosidase together.
  20. The method according to Claim 17, wherein said adding comprises adding the pectinase and the beta-galactosidase separately.
  21. The method according to Claim 17, wherein said adding comprises adding the pectinase and the beta-galactosidase in a relative ratio ranging from about 100:1 to about 1:100 wherein the amount of the pectinase and beta-galactosidase ranges from about 0.01 to about 50 wt% based on the total amount of the ginseng solution or ginseng.
  22. The method according to Claim 17, wherein said adding comprises adding the pectinase and the beta-galactosidase to the ginseng solution at a temperature of from about 10℃ to about 70℃.
  23. The method according to Claim 17, wherein said adding comprises adding the pectinase and the beta-galactosidase to the ginseng solution for a time period from about 1 to about 72 hours.
  24. The method according to Claim 17, wherein the first and second solvents are one or more of an aqueous solvent, organic solvent, and a mixture thereof.
  25. The method according to Claim 17 further comprising:
    drying the fermented ginseng concentrate after said concentrating to produce fermented ginseng powder.
  26. The method according to Claim 25, wherein said drying is carried out by drying under reduced pressure, hot-air drying, spray drying, fluidized bed drying, fluidized bed granulation, or freeze drying.
  27. A fermented ginseng concentrate prepared by the method according to Claim 17.
  28. A food composition comprising the fermented ginseng concentrate of Claim 27.
  29. A functional food composition comprising a therapeutically effective amount of the fermented ginseng concentrate of Claim 27.
  30. A fermented ginseng powder prepared by the method according to Claim　25.
  31. A food composition comprising the fermented ginseng powder of Claim　30.
  32. A functional food composition comprising a therapeutically effective amount of the fermented ginseng powder of Claim 30.
PCT/KR2009/002635 2009-05-19 2009-05-19 Methods for preparing a fermented ginseng concentrate or powder WO2010134650A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP09844959.8A EP2432891B1 (en) 2009-05-19 2009-05-19 Methods for preparing a fermented ginseng concentrate or powder
PCT/KR2009/002635 WO2010134650A1 (en) 2009-05-19 2009-05-19 Methods for preparing a fermented ginseng concentrate or powder
JP2012511737A JP6170674B2 (en) 2009-05-19 2009-05-19 Method for producing fermented ginseng concentrate or powder
CN200980160042.9A CN102459623B (en) 2009-05-19 2009-05-19 Methods for preparing a fermented ginseng concentrate or powder

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR2009/002635 WO2010134650A1 (en) 2009-05-19 2009-05-19 Methods for preparing a fermented ginseng concentrate or powder

Publications (1)

Publication Number Publication Date
WO2010134650A1 true WO2010134650A1 (en) 2010-11-25

Family

ID=43126315

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/002635 WO2010134650A1 (en) 2009-05-19 2009-05-19 Methods for preparing a fermented ginseng concentrate or powder

Country Status (4)

Country Link
EP (1) EP2432891B1 (en)
JP (1) JP6170674B2 (en)
CN (1) CN102459623B (en)
WO (1) WO2010134650A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101409761B1 (en) * 2012-06-20 2014-06-19 주식회사한국야쿠르트 A method of preparation for fermented red ginseng using conversion by enzyme mixture and fermentation by lactic acid bacterium and the products containing fermented red ginseng manufactured thereof as effective factor
KR101493733B1 (en) 2013-11-27 2015-02-16 한국식품연구원 Ginseng rice beverage containing rice ferment material and method for manufacturing thereof
KR20160072802A (en) * 2014-12-15 2016-06-23 경성대학교 산학협력단 Pharmaceutical Composition Including Ginseng Saponin As Active Ingredient
KR20170047007A (en) * 2015-10-22 2017-05-04 (주)아모레퍼시픽 Method for selectively preparing ginsenoside F2, compound Mc and compound O from saponins of ginseng by enzymatic process
KR101633428B1 (en) * 2016-01-14 2016-06-27 주식회사 진산사이언스 Manufacturing Method for Power of Red Ginseng or Ginseng Extract
KR101794860B1 (en) * 2016-01-29 2017-11-09 주식회사 홍삼단 Manufacturing method of spherical Red Ginseng Extract granules using Red Ginseng Extract powder and fluidized bed granulation coating device
CN106072567A (en) * 2016-07-01 2016-11-09 谢少韩 A kind of ginseng product and preparation technology thereof
KR20230072108A (en) * 2021-11-17 2023-05-24 주식회사 엘지생활건강 Manufacturing method of the red ginseng concentrate with improved palatability

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2443266A1 (en) 1978-12-07 1980-07-04 Cotte Jean Marie Extracting ginseng e.g. by maceration - with enzyme treatment before maceration
KR19980066516A (en) * 1997-01-25 1998-10-15 윤형완 Method of extracting active ingredient including saponin contained in plant by enzyme treatment
KR20020009756A (en) 2000-07-26 2002-02-02 박명규 Manufacturing Method for Ginsenoside Compound K by Enzymatic Reaction
KR20030037005A (en) * 2001-11-01 2003-05-12 주식회사 태평양 Manufacturing method of Compound K and Ginsenoside F1 from ginseng ginsenosides
KR20030094757A (en) 2002-06-07 2003-12-18 주식회사 비티진 Process for preparation of ginsenoside-F2, Compound-K using β-glycosidase
US20060160752A1 (en) 2002-12-26 2006-07-20 Su-Jong Kim Promoter for the production of hyaluronic acid containing ginsenoside compound k
WO2009057836A1 (en) 2007-10-31 2009-05-07 Amorepacific Corporation Use of melanin biosynthesis inhibitors from korean ginseng and the cosmetic composition containing thereof for skin whitening

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61289853A (en) * 1985-06-17 1986-12-19 Yakult Honsha Co Ltd Extraction of panax ginseng extract
JP2002348245A (en) * 2001-05-07 2002-12-04 Toyo Hakko:Kk Method for changing effective ingredients of ginseng radix rubra into low molecular weight compounds, and method for preparing antioxidizing drink
CN1167805C (en) * 2001-11-06 2004-09-22 中国科学院大连化学物理研究所 Ginsenoside enzymatically hydrolyzing process to prepare 2o-beta-D-pyranoglucose protopanoxadiol
JP4549625B2 (en) * 2002-01-05 2010-09-22 株式會社アモーレパシフィック Finely emulsified particles containing ginseng saponin metabolites as active ingredients, a method for producing the same, and a cosmetic composition for preventing skin aging containing the same
CN1240390C (en) * 2002-01-30 2006-02-08 株式会社一和 Biologic converted ginseng composition and preparing process thereof
JP3678362B2 (en) * 2002-07-23 2005-08-03 長谷川 秀夫 Method for producing fermented carrot containing saponin degradation products
KR100618171B1 (en) * 2003-04-30 2006-08-29 김재백 Fermented red ginseng containing ginseng saponin degradate and its manufacturing method
JP4939936B2 (en) * 2003-06-27 2012-05-30 株式會社アモーレパシフィック Method for producing self-assembling polymer nanoparticles containing physiologically active ingredients, and external preparation composition containing the obtained self-assembling polymer nanoparticles
CN100586444C (en) * 2003-09-30 2010-02-03 涌永制药株式会社 Method of processing ginseng for medicinal use and composition
JP2005160373A (en) * 2003-12-02 2005-06-23 Hideo Hasegawa Glycoside-degradative probiotics
KR101096372B1 (en) * 2004-04-14 2011-12-20 (주)아모레퍼시픽 Polymer capsules containing ginsenoside and a process for preparation thereof, and a cosmetic composition containing the capsules
CN1899336A (en) * 2006-07-04 2007-01-24 辽宁中医药大学 Method for preparing ginseng anti-cancer active component by immobilized enzyme
JP5204771B2 (en) * 2007-06-21 2013-06-05 長瀬産業株式会社 GABAergic neuron activator

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2443266A1 (en) 1978-12-07 1980-07-04 Cotte Jean Marie Extracting ginseng e.g. by maceration - with enzyme treatment before maceration
KR19980066516A (en) * 1997-01-25 1998-10-15 윤형완 Method of extracting active ingredient including saponin contained in plant by enzyme treatment
KR20020009756A (en) 2000-07-26 2002-02-02 박명규 Manufacturing Method for Ginsenoside Compound K by Enzymatic Reaction
KR100377546B1 (en) 2000-07-26 2003-03-26 주식회사 케이티앤지 Manufacturing Method for Ginsenoside Compound K by Enzymatic Reaction
KR20030037005A (en) * 2001-11-01 2003-05-12 주식회사 태평양 Manufacturing method of Compound K and Ginsenoside F1 from ginseng ginsenosides
KR100418604B1 (en) 2001-11-01 2004-02-11 주식회사 태평양 Manufacturing method of Compound K and Ginsenoside F1 from ginseng ginsenosides
KR20030094757A (en) 2002-06-07 2003-12-18 주식회사 비티진 Process for preparation of ginsenoside-F2, Compound-K using β-glycosidase
US20060160752A1 (en) 2002-12-26 2006-07-20 Su-Jong Kim Promoter for the production of hyaluronic acid containing ginsenoside compound k
WO2009057836A1 (en) 2007-10-31 2009-05-07 Amorepacific Corporation Use of melanin biosynthesis inhibitors from korean ginseng and the cosmetic composition containing thereof for skin whitening

Non-Patent Citations (24)

* Cited by examiner, † Cited by third party
Title
AKAO ET AL., BIOL. PHARM. BULL., vol. 21, 1998, pages 245 - 9
BAE ET AL., J. MICROBIAL. BIOTECHNOL., vol. 13, pages 9 - 14,2003
CHANG: "Pharmacology and Application of Chinese Material Medica", vol. 1, 1986, WORLD SCIENTIFIC
CHEN ET AL., CHEM. PHARM. BULL., vol. 35, 1987, pages 1653 - 5
CHOI ET AL., J. GINSENG RES., vol. 31, no. 2, 2007, pages 79 - 85
DO YEON KIRN: "Fermented ginseng attenuates hepatic lipid accumulation and hyperglycemia through AMPK activation", FOOD SCIENCE AND BIOTECHNOLOGY, vol. 18, no. 1, 1 January 2009 (2009-01-01), pages 172 - 178
ELYAKOV ET AL.: "Synthesis of the Ginseng Glycosides and Their Analogs", PROC. 6 THINT. GINSENGSYMP., 1993, pages 74 - 83
HAN ET AL., PLANTA MEDICO, vol. 44, 1982, pages 146 - 9
HASEGAWA ET AL., PLANTA MEDICA, vol. 63, 1997, pages 463 - 40
HUANG: "The Pharmacology of Chinese Herbs", 1999, CRC PRESS
KANAODA ET AL., J, TRADIT. MED, vol. 11, 1994, pages 241 - 5
KARIKURA ET AL., CHEM. PHARM. BULL., vol. 38, 1990, pages 2859
KARIKURA ET AL., CHEM. PHARM. BULL., vol. 39, 1991, pages 2357 - 61
KO ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 64, 2000, pages 2739 - 43
KO ET AL., PLANTA MED., vol. 69, 2003, pages 285 - 6
LEE ET AL., CANCER LETT., vol. 144, 1999, pages 39 - 43
LEE: "Factsabout Ginseng, the Elixir of Life", 1992, HOLLYO INTERNATIONAL
MOCHIZUKIET, BIOL. PHARM. BULL., vol. 18, 1995, pages 1197 - 1202
SATO ET AL., BIOL. PHARM. BULL., vol. 17, 1994, pages 635 - 9
See also references of EP2432891A4
TAWAB ET AL., DRUG METAB. DISPOS., vol. 31, 2003, pages 1065 - 71
TAWAB ET AL., DRUGMETAB. DISPOS., vol. 31, 2003, pages 1065 - 71
WAKABAYASHI ET AL., ONCOL. RES., vol. 9, 1998, pages 411 - 7
WU ET AL., J. IMMUNOL., vol. 148, 1992, pages 1519 - 25

Also Published As

Publication number Publication date
EP2432891A4 (en) 2015-04-22
CN102459623B (en) 2015-06-24
JP6170674B2 (en) 2017-07-26
EP2432891B1 (en) 2018-04-04
JP2012527233A (en) 2012-11-08
EP2432891A1 (en) 2012-03-28
CN102459623A (en) 2012-05-16

Similar Documents

Publication Publication Date Title
WO2010134650A1 (en) Methods for preparing a fermented ginseng concentrate or powder
WO2013058594A2 (en) Method for preparing a fermented deer antler extract having an anti-inflammatory activity, extract obtained by the method and use of such extract
WO2010137846A2 (en) Composition for increasing the bioavailability of saponin
WO2018139898A1 (en) Composition for enhancing immunity, containing ginseng berry polysaccharides
WO2014027742A1 (en) Fermented ginseng or fermented red ginseng having enhanced ginsenoside rd, and method for preparing same
WO2021230581A1 (en) Discovery of novel akkermansia muciniphila ak32 and application thereof for prevention or treatment of intestinal injury
WO2017061769A1 (en) Composition for preventing alopecia or stimulating hair growth containing extracellular polysaccharide produced from ceriporia lacerata as active ingredient
WO2018043874A1 (en) Composition, for preventing hair loss or promoting hair growth, comprising strains showing lipolysis effect
WO2017026641A1 (en) Composition for preventing or treating cervical cancer including gypenoside lxxv
WO2021010791A1 (en) Composition for alleviating, preventing, or treating cardiovascular disorders, containing bioconverted salvia miltiorrhiza extract and bioconverted red ginseng extract
WO2013062247A2 (en) Phorbol type diterpene compound, pharmaceutical composition for treatment or prevention of viral infectious diseases including same
WO2017176001A1 (en) Composition for alleviating menopausal symptoms in women, containing sasa quelpaertensis nakai extract
WO2014133276A1 (en) Composition containing, as active ingredients, menispermum dauricum, extract of menispermum dauricum, slime of menispermum dauricum, or extract of malt enzyme liquid of menispermum dauricum for preventing or treating anorexia
WO2017018791A1 (en) Ginseng fruit extract containing alcoholic liver damage preventive functional ingredient, and preparation method therefor
US8541035B2 (en) Methods for preparing a fermented ginseng concentrate or powder
WO2021010790A1 (en) Anticancer composition comprising salvia miltiorrhiza bioconversion extract and red ginseng bioconversion extract
WO2011059212A2 (en) Preparation method of ginseng and red ginseng concentrates containing the increased amount of compound k by using pectinex
WO2019240508A1 (en) Novel compound isolated from acanthopanax sp. fruit extract, and pharmaceutical composition for preventing and treating hypertension, containing same
WO2018131780A1 (en) Composition for preventing or treating gastritis or peptic ulcer
WO2016175559A2 (en) Composition for preventing hair loss and improving hair growth, containing complex extract of anemarrhena asphodeloides bunge and aralia continentalis kitagawa
WO2019245245A1 (en) Pharmaceutical composition for preventing and treating liver damage comprising turmeric extract
KR100954851B1 (en) Preparation method of fermented ginseng concentrate or powder having high concentration of IH-901
WO2023146123A1 (en) Composition for preventing or treating inflammatory diseases comprising composite extract of ocimum basilicum, cirsium nipponicum (maxim.) makino, and aruncus dioicus as active ingredient
WO2023224158A1 (en) Method for preparing milk thistle extract having increased silymarin bioavailability
WO2020004712A1 (en) Zinc-containing, water-soluble polyglutamic acid complex composition

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200980160042.9

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09844959

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2012511737

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2009844959

Country of ref document: EP