JPS61289853A - Extraction of panax ginseng extract - Google Patents

Abstract

PURPOSE:To obtain Panax ginseng extract in a short time in high yield, by extracting Panax ginseng after the treatment with cellulase or pectinase. CONSTITUTION:In the extraction of Panax ginseng, the Panax ginseng to be extracted is treated with cellulase or pectinase before extraction. Raw Panax ginseng may be used for the extraction raw material, however, it is preferable to use a dried Panax ginseng to obtain uniform powder and enable the enzymatic treatment under stable condition. The enzymatic treatment is carried out by suspending crushed Panax ginseng in about 5 times volume of water. In the case of using dried Panax ginseng, the Panax ginseng powder can be swollen to promote the impregnation of the enzyme and to attain the treatment effect in short time by heating the Panax ginseng in boiling water for about 10min before the treatment. The preferable amount of the enzyme depends upon the titer of the enzymatic agent and is about 1.25%, generally about 1.0-4.0% based on the dried raw material in the case of an enzymatic agent having the most preferable combination.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention relates to an improved method for extracting ginseng extract.

BACKGROUND OF THE INVENTION Panax ginseng (Panax ginsen H) is a perennial herb native to China and is well-known as one of the most important herbal medicines in old rice, and its root extract is widely used as a tonic. The active ingredient of Panax ginseng as a crude drug is saponin called synthside, and Panax ginseng root contains about 0.3 to 3% of its fresh weight.

The conventional method for industrially extracting ginseng extract is to extract raw ginseng or dried ginseng powder with ethanol, and then press it mechanically. The solid content ratio is 21-27%
That's about it. However, this extraction method not only takes a long time to extract, but also has low yields, and there are problems in that many active ingredients are wasted without being extracted.

Problems to be Solved by the Invention In view of the fact that the conventional ginseng extract extraction method had the above-mentioned drawbacks, the present invention aims to provide a higher extract yield in a shorter time. The purpose is to improve the extraction method.

Means for Solving the Problems The method for extracting Korean ginseng extract of the present invention, which has succeeded in achieving the above object, involves extracting the extract from Korean ginseng by a conventional method. It is characterized by treatment with pectinase.

As the enzyme used for pretreatment in the method of the present invention, any commercially available cellulase agent or pectinase agent can be used, and both may be used in combination.

The ginseng to be enzyme-treated may be fresh, but dried ginseng has the advantage that it becomes a uniform powder when crushed, and the enzyme treatment can be carried out under stable conditions.

Enzyme treatment is performed by suspending ground ginseng in approximately 5 times the volume of water; however, in the case of dried ginseng, steaming it in a boiling water bath for approximately 10 minutes before treatment produces ginseng powder. swells, making it easier for enzymes to penetrate, resulting in high treatment effects in a short period of time. The preferred amount of enzyme added varies depending on the potency of the enzyme agent used, but when using the best combination of enzyme agents, it is approximately 1.25% based on the dry raw material, generally 1.
．． It is about 0 to 4.0%. Generally, the higher the amount of enzyme added, the higher the extract yield. When cellulase and pectinase are used in combination, the ratio by weight of the former is preferably about 1 to about 0.6 to 1.5 of the latter. A suitable temperature for enzyme treatment is about 34 to 54°C, and the maximum extraction rate is achieved at about 50°C. pH
is desirably adjusted to approximately 4.25 to 5.55, and most preferably pi-( is approximately 4.5 (in the case of no adjustment,
pH will be approximately 5.0-5.5).

The treatment time is about 3 hours or more, preferably about 4 to 5 hours.

One way to stop the enzymatic reaction is to perform a boiling water bath treatment for 10 minutes, for example.

The extraction treatment of Panax ginseng after the enzyme treatment as described above is performed as a pretreatment may be the same as a conventional method. A typical method is to add ethanol to a ginseng suspension after enzyme treatment to make an approximately 60-80% ethanol suspension, and to
There is a method of heating to ~75°C for 4 to 6 hours, filtering, and evaporating the filtrate to dryness.

The Korean ginseng extract obtained by the method of the present invention has almost no difference in quality from the extract obtained without enzyme treatment. The end result is that it can be used as a herbal medicine.

Effects and Effects According to the method of the present invention, macromolecular substances such as cellulose and pectin are hydrolyzed by cellulase or pectinase and the carrot tissue is loosened, so extracts containing saponin can be extracted in a shorter time than with conventional methods. This is done at a high rate. The resulting Korean ginseng extract sapon (Ginsesside Rb)
, and Rg + ) content is slightly lower than that of the extract obtained without enzyme treatment, but the overall saponin extraction rate increases because the extract yield is significantly increased.

Therefore, according to the present invention, the utilization rate of expensive Korean ginseng is improved, and its extract can be provided at a lower cost than before.

x unusual case The present invention will be explained below with reference to experimental examples and examples.

Experimental Example 1 Changes in the extraction rate of dried Korean ginseng extract due to the use of various cellulase agents and pectinase agents alone were investigated.

Dried Korean ginseng was finely ground using a mixer, 2 g of it was suspended in 8 ml of water, and the mixture was steamed in a boiling water bath for 10 minutes. After cooling, 2 ml of enzyme solution with a concentration of 1.25% or 2.5%
(2 ml of water for control) was added (1.25% or 2 ml of ginseng).
．． Add 1 5% enzyme) and react at 50°C for 3 hours. After stopping the enzyme reaction by boiling water bath treatment for 10 minutes, 23 ml of 99.5% ethanol was added and
Heat to ℃ for 5 hours. After cooling, it is filtered and the residue is washed with 17 ml of 70% ethanol. The combined filtrate and washings are evaporated to dryness and further dried at 105°C.

In the above extraction method, Macerozyme S (Rhizopus sp, origin, Yakult Honsha) and Pectinase SS (Rhizopus sp., Yakult Honsha) were used as pectinase agents.
pectinase G ``Amano'' (derived from Aspergillus niger, Amano Pharmaceutical) or pectinase
Onozuka J3S (Tri-
(derived from choderma viride, Yakult Honsha)
, cellulase YNC (Aspergillus nig
er Yume, Yakult Honsha), Driselase (Irpex)
1acteus, Kyowa Hakko) or cellulase
Table 1 shows the extract extraction rate when using "Amano" A3 (derived from Spergillus niger, Amano Pharmaceutical).

Table 1 Experimental example of change in extract yield [%] due to the use of enzymes 2 Cellulase and pectinase were used for ginseng], 2
Panax ginseng was treated and extracted in the same manner as in Experimental Example 1 using 5% addition amount, and the results shown in Table 2 were obtained.

Table 2 Extract yield when enzymes are used together Enzymes used
Extract yield [%] Pectinase SS + Cellulase YNC52,1 Pectinase SS + Cellulase "Onozuka J3S 4.6.7 Pectinase SS
+ Cellulase "Amano" 83 44.5 Macerozyme S + Cellulase YNC 4,4,5 Macerozyme S + Cellulase "Onozuka J3S 39.0 Macerozyme S + Dricelase 45.6 Pectinase C "Amano" + Cellulase YNC 48,0 Pectinase C "Amano" + Cellulase 341 to “Amano”
．． 1 Pectolyase + Cellulase YNC51,9 Pectolyase + Driselase 39.3 Experimental Example 3 Extract the bearded root part and radish part of Korean ginseng in the same manner as in Experimental Example 1 except that the following enzymes were used. (all enzyme amounts are % by weight of ginseng).

Enzyme treatment 1: Pectinase SS 1.25%, Cellulase YNC 1.25% Enzyme treatment 2: Pectinase SS 1.50%, Cellulase YNC 1.50% The obtained extract and the same control example without enzyme treatment Regarding the extract, Ginsside R1]1 and Ginsside Rg+ were determined by gas chromatography.

The results are shown in Table 3.

Table 3 Bearded root 9.4 3,2 12,6 24.7
3.11 None Radish 1.9 1.3 3,2
26,7 0.851 Beard root 6.4 2.0
8.4 51.2 4.301 Thick root 1.5
0.9 2.4 51.6 1.242 Beard root 53.72 Radish
58.28 Example 1 After crushing Ikg of dried Korean ginseng with a mixer to a powder that can pass through a 35-mesh sieve, add 4kg of water and
Steamed at °C for 10 minutes. After cooling, add 4% aqueous solution 10Q of each of pectinase SS and cellulase YNC.
ml was added, the pH was adjusted to 4.5 with 1/2ON acetate buffer, and the enzyme reaction was carried out at 50°C for 5 hours. Then, after steaming in a boiling water bath for 10 minutes to stop the enzyme reaction,
11.5Q of 99.5% ethanol was added, and the extract was extracted by heating at 70°C for 5 hours. After cooling and filtration, the filtrate was evaporated to dryness and further dried at 105°C to obtain 681 g of an extract with a ginsefside content of 2.0%.

Claims (1)

[Claims] A method for extracting a Korean ginseng extract, which comprises treating the Korean ginseng to be subjected to extraction treatment with cellulase or pectinase before extraction.