WO2010113949A1 - Procédé de production de solution saccharifiée d'origine céréalière - Google Patents

Procédé de production de solution saccharifiée d'origine céréalière Download PDF

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Publication number
WO2010113949A1
WO2010113949A1 PCT/JP2010/055707 JP2010055707W WO2010113949A1 WO 2010113949 A1 WO2010113949 A1 WO 2010113949A1 JP 2010055707 W JP2010055707 W JP 2010055707W WO 2010113949 A1 WO2010113949 A1 WO 2010113949A1
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WO
WIPO (PCT)
Prior art keywords
saccharification
cereal
saccharified solution
producing
starch
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PCT/JP2010/055707
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English (en)
Japanese (ja)
Inventor
正幸 遠山
一栄 高岡
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三井造船株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Priority to JP2011507215A priority Critical patent/JP4818482B2/ja
Publication of WO2010113949A1 publication Critical patent/WO2010113949A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • the present invention relates to a method for producing a saccharified cereal liquid suitable for continuous ethanol production.
  • biofuels especially bioethanol
  • Phenomena such as the development of a car that uses ethanol as fuel and the rising prices of corn and sugarcane as raw materials are new to memory.
  • grains such as rice, brown rice, and wheat are familiar materials for fermenting alcoholic beverages, but they are more expensive than corn and sugarcane, have a high protein and lipid content, Utilization as a raw material for industrial ethanol production was delayed due to starch gelatinization.
  • a cereal-derived ethanol production process comprises a saccharification process in which starch contained in a raw material is hydrolyzed to a sugar such as glucose using an enzyme or the like, and a fermentation process in which ethanol is produced from glucose by a microorganism.
  • This ⁇ starch is less susceptible to degradation by saccharifying enzymes due to steric hindrance and the like.
  • starch in a state where the crystal structure is collapsed and each branch of starch molecules is spread in water is called ⁇ starch, and changing the state of ⁇ starch to ⁇ starch is called starch pregelatinization.
  • the present inventor considered providing a pretreatment step for pregelatinizing the starch contained in the raw material before subjecting it to the saccharification step, and has so far disclosed the method disclosed in Patent Document 1.
  • an object of the present invention is to provide a method for producing a cereal-derived saccharified solution suitable for continuous ethanol production at a low cost and without requiring the addition of a fermentation auxiliary material.
  • the invention according to claim 1 is a method for producing a saccharification liquid derived from cereals suitable for continuous ethanol production having a saccharification step for saccharifying starch contained in cereals.
  • the invention described in claim 2 is the method for producing a cereal-derived saccharified solution suitable for continuous ethanol production according to claim 1, wherein the saccharification inhibiting factor is a protein and a lipid.
  • the invention according to claim 3 is the method for producing a cereal-derived saccharified solution suitable for continuous ethanol production according to claim 1 or 2, wherein the saccharification inhibiting factor conversion treatment is an enzyme treatment with protease and lipase. is there.
  • the invention according to claim 4 is characterized in that the fermentation by-products are raw materials providing a nitrogen source and a phosphorus source, and the saccharification derived from grains suitable for continuous ethanol production according to any one of claims 1 to 3 It is a manufacturing method of a liquid.
  • the invention according to claim 5 is the production of a cereal-derived saccharified solution suitable for continuous ethanol production according to any one of claims 1 to 4, wherein the cereal contains at least one of rice, brown rice and wheat. Is the method.
  • the invention according to claim 6 is the method for producing a cereal-derived saccharified solution suitable for continuous ethanol production according to any one of claims 1 to 5, wherein the cereal is powdered cereal flour. .
  • the present invention is characterized in that a saccharification inhibitor conversion process for converting a saccharification inhibitor that inhibits saccharification of starch into a fermentation auxiliary material is performed before the saccharification step.
  • the saccharification step is a step of converting starch into a sugar such as glucose that can be used by fermentation microorganisms (for example, yeast), and before the saccharification step is a pretreatment step of performing liquefaction or hydrolysis, for example.
  • the saccharification-inhibiting factor conversion treatment of the present invention preferably includes performing in a pretreatment step before the saccharification step and further before the pretreatment step, and preferably performing the saccharification inhibitory factor conversion treatment in the liquefaction step.
  • the degradation of the saccharification inhibiting factor, particularly protein does not proceed. The effect by cannot be obtained.
  • a method for producing a saccharified cereal derived from cereals suitable for continuous ethanol production includes firstly converting the starch contained in the cereal into ⁇ , and further saccharifying the starch.
  • the saccharification inhibitor is removed by glycation inhibitor conversion.
  • saccharification inhibitor examples include proteins, lipids, lignocellulose and the like.
  • saccharification-inhibiting factors are present around the starch in the cereal and act as a steric hindrance to the water required for the saccharification and the saccharifying enzyme that saccharifies, thereby inhibiting the saccharification of the starch.
  • saccharification inhibiting factor conversion treatment for removing the saccharification inhibiting factor
  • examples of the saccharification inhibiting factor conversion treatment for removing the saccharification inhibiting factor include enzyme treatment, acid treatment, chemical treatment, high temperature treatment, pressure treatment, etc.
  • enzyme treatment it is preferable to use enzyme treatment.
  • the enzyme treatment exhibits a sufficient effect even under mild reaction conditions, and acts only on the saccharification inhibiting factor due to the substrate specificity, so that there is no adverse effect such as inappropriate modification of starch to saccharification or fermentation.
  • the starch can be efficiently ⁇ -converted, and the saccharifying enzyme can be efficiently converted to ⁇ -starch without being inhibited by steric hindrance.
  • a saccharified solution having a high sugar concentration can be obtained.
  • the cereal used in the present invention is not limited as long as it is a cereal, but is preferably a cereal having a high content of proteins and lipids that are glycation inhibitors and a low lignocellulose content, and particularly, rice, brown rice, wheat and the like are suitable. . These grains are preferably cut and pulverized into powder or chips of an appropriate size. Furthermore, these grains may be used alone or in combination.
  • cereal grains having a low content of saccharification inhibitor can be suitably used for the production of saccharified liquid.
  • cereals having a high content of saccharification inhibitors are preferably used in the present invention because the saccharification inhibitor conversion process not only removes saccharification inhibitors but also fermentation. It is because it has the effect
  • the fermentation auxiliary material refers to a raw material required in addition to sugar as a fermentation raw material in microbial fermentation, and examples thereof include a raw material that provides a nitrogen source and a phosphorus source.
  • the saccharification inhibiting factor conversion treatment in the present invention generates peptides, amino acids, fatty acids and the like by decomposing proteins and lipids that are difficult for microorganisms to use. These are suitable as fermentation by-products because they have a reasonably low molecular weight and are easily used by microorganisms and contain abundant nitrogen and phosphorus.
  • the saccharified solution obtained by the present invention contains abundantly both the sugar that is the fermentation raw material and the nitrogen source and the phosphorus source that are the fermentation auxiliary raw materials, it is necessary to add the fermentation auxiliary raw material separately in the fermentation process. Without sufficient fermentation performance.
  • the method for producing a cereal-derived saccharified solution according to the present invention can be suitably used for continuous ethanol production.
  • continuous ethanol production refers to continuous production of saccharified liquid to the fermentation process while continuously taking out the produced ethanol.
  • the glycation inhibiting factor conversion treatment in the present invention is preferably an enzyme treatment, and in particular, an enzyme treatment with a protease that is a proteolytic enzyme and a lipase that is a lipolytic enzyme is suitable.
  • the protease used in the present invention may be any enzyme that can degrade proteins, and examples include exopeptidase, endopeptidase, proteinase, and peptidase.
  • the protease may be a combination of several types of proteases.
  • the lipase used in the present invention may be any enzyme that can degrade lipids.
  • the lipase may be used in combination of several lipases.
  • grains such as rice, brown rice, and wheat have a low lignocellulose content. Therefore, glycation is inhibited only by allowing protease and lipase to act under mild temperature conditions (around 50 ° C.) without using lignocellulose-degrading enzyme. The factor can be removed sufficiently.
  • lignocellulose-degrading enzyme in addition to enzyme treatment with protease and lipase, lignocellulose-degrading enzyme may be used in combination.
  • the lignocellulose covers the starch firmly, so the removal of the saccharification inhibitor is insufficient even if the enzyme treatment is performed using an enzyme containing a lignocellulose-degrading enzyme.
  • a sugar as a fermentation raw material is obtained by decomposition of lignocellulose, a nitrogen source or a phosphorus source as a fermentation auxiliary raw material cannot be obtained. Therefore, the application of the present invention is difficult.
  • the saccharification inhibiting factor conversion treatment may be performed as a pretreatment before the saccharification step for saccharifying starch, or may be performed simultaneously with the saccharification step.
  • the saccharification inhibiting factor conversion treatment performed before the saccharification step may be continuously performed in the saccharification step.
  • amylase may be allowed to act after the protease and lipase are allowed to act on starch, or the starch may be saccharified at any time by causing protease, lipase and amylase to act on starch simultaneously. May be.
  • the saccharification inhibiting factor conversion treatment performed as the pretreatment may be terminated before shifting to the saccharification step. If the saccharification inhibiting factor conversion treatment is an enzyme treatment, the treatment can be easily terminated by raising the temperature to the enzyme deactivation temperature.
  • the raw material flour, water and protease and lipase as saccharification inhibiting factor conversion means are introduced into a container at a predetermined mixing ratio, mixed and stirred, and this is stirred for a predetermined time 45 to 45. Keep in the range of 55 ° C.
  • the mixing ratio (weight ratio) of cereal flour and water is preferably 1: 1.5 to 2.5, more preferably 1: 1.2 to 2.2.
  • the mixing ratio (weight ratio) exceeds 1: 2.5, the enzyme reaction effect decreases, which is not preferable.
  • the mixing ratio (weight ratio) is less than 1: 1.5, the concentration of the resulting saccharified solution decreases.
  • the mixing ratio (weight ratio) of cereal flour and protease and lipase is 1: 0.0004 to 0.001 for cereal flour and protease, and 1: 0.0004 to 0.001 for cereal flour and lipase. More preferably, cereal flour and protease are from 1: 0.0006 to 0.0008, and cereal flour and lipase are from 1: 0.0006 to 0.0008. Protease and lipase may be mixed in a lower amount than the above mixing ratio. The mixture is allowed to stand for 2 hours while maintaining a temperature in the range of 45 to 55 ° C. to convert the saccharification inhibitor (decomposition of proteins and lipids) and to gelatinize starch. Subsequently, after adding amylase, it is left to stand for about 2 hours to decompose the ⁇ starch into glucose.
  • Example 1 300 g of wheat flour, 0.2 g of protease (Protease N “Amano”), 0.2 g of lipase (Wako) and 600 g of water were placed in a container, stirred, and then heated at 50 ° C. for 30 minutes.
  • the weight ratio of flour: protease: lipase: water is 1: 0.0007: 0.0007: 2.
  • liquefying enzyme asmylase RG-IIG
  • the enzyme was inactivated by heating at 100 ° C. for 30 minutes.
  • a wheat-derived saccharified solution having a sugar concentration of 300 g / L (in terms of glucose) was obtained.
  • the glucose concentration was measured by high performance liquid chromatography (HPLC LC-8010, manufactured by Tosoh Corporation).
  • the obtained wheat-derived saccharified solution was supplied to a fermentor, and ethanol was continuously produced without adding fermentation auxiliary materials.
  • the pH of the flour solution was adjusted to 5.8 with an aqueous NaOH solution (10 mol / L).
  • Liquefaction enzyme (Amylase RG-IIG) was added to the raw wheat flour at 0.05 wt% for liquefaction.
  • a wheat-derived saccharified solution having a sugar concentration of 300 g / L (in terms of glucose) was obtained.
  • the glucose concentration was measured by high performance liquid chromatography (HPLC LC-8010, manufactured by Tosoh Corporation).
  • the obtained saccharified wheat-derived saccharified solution was supplied to the same fermentor as in Example 1, and ethanol was continuously produced without adding any fermentation auxiliary materials.
  • Comparative Example 2 The wheat-derived saccharified solution obtained in Comparative Example 1 was supplied to the same fermentor as in Example 1, and a 1% corn steep liquor (CSL) solution was added as a fermentation auxiliary material to continuously produce ethanol.
  • CSL corn steep liquor
  • Comparative Example 2 is obtained by adding a fermentation auxiliary material (CSL) to the sugar solution obtained in the same manner as Comparative Example 1, and subjecting it to a fermentation process.
  • a fermentation broth having an ethanol concentration of 100 to 150 g / L was obtained. That is, it is thought that fermentation by yeast was successfully performed by adding the fermentation auxiliary material.
  • Example 2 In the saccharified solution obtained in Example 1, no fermentation auxiliary material was added, but a fermentation solution having an ethanol concentration of 100 to 150 g / L was obtained as in Comparative Example 2. Since the nitrogen source and the phosphorus source were supplied by performing the saccharification inhibiting factor conversion treatment, it was considered that the fermentation was successfully performed without the addition of the fermentation auxiliary material.

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
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  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention porte sur un procédé de production d'une solution saccharifiée d'origine céréalière appropriée pour un processus de production d'éthanol continu qui peut être réalisé à un coût faible et un rendement élevé sans ajouter aucune matière de fermentation auxiliaire. L'invention porte spécifiquement sur un procédé de production d'une solution saccharifiée d'origine céréalière approprié pour un processus de production d'éthanol continu, qui comprend une étape de saccharification pour saccharifier l'amidon contenu dans la céréale, caractérisé en ce qu'un traitement de conversion d'inhibiteur de saccharification pour convertir un inhibiteur de saccharification, qui inhibe la saccharification de l'amidon, en une matière de fermentation auxiliaire, est effectué avant l'étape de saccharification.
PCT/JP2010/055707 2009-03-30 2010-03-30 Procédé de production de solution saccharifiée d'origine céréalière WO2010113949A1 (fr)

Priority Applications (1)

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JP2011507215A JP4818482B2 (ja) 2009-03-30 2010-03-30 穀物由来糖化液の製造方法

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JP2009-081663 2009-03-30
JP2009081663 2009-03-30

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008535523A (ja) * 2005-04-12 2008-09-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 標的化学物質を得るためのバイオマス処理

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008535523A (ja) * 2005-04-12 2008-09-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 標的化学物質を得るためのバイオマス処理

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
LABEILLE, P. ET AL.: "Comparative study of wheat flour saccharification and ethanol production with two glucoamylase preparations.", INDUSTRIAL CROPS AND PRODUCTS, vol. 6, 1997, pages 291 - 295 *
PEREZ-CARRILLO, ESTHER. ET AL.: "Effect of Sorghum Decortication and Use of Protease Before Liquefaction with Thermoresistant a-Amylase on Efficiency of Bioethanol Production", CEREAL CHEMISTRY, vol. 85, 2008, pages 792 - 798 *
WU, XIAORONG. ET AL.: "Factors Adversely Affecting Ethanol Production in the Dry Grind Processing of Sorghum", PAPERS. AMERICAN SOCIETY OF AGRICULTURAL AND BIOLOGICAL ENGINEERS, 2007, pages 1 - 11 *

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JP4818482B2 (ja) 2011-11-16

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