WO2010100795A1 - Système de brassage pour photosynthèse, photobioréacteur utilisant ce système de brassage et procédé de culture d'organismes aquatiques utilisant ce photobioréacteur - Google Patents

Système de brassage pour photosynthèse, photobioréacteur utilisant ce système de brassage et procédé de culture d'organismes aquatiques utilisant ce photobioréacteur Download PDF

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WO2010100795A1
WO2010100795A1 PCT/JP2009/069137 JP2009069137W WO2010100795A1 WO 2010100795 A1 WO2010100795 A1 WO 2010100795A1 JP 2009069137 W JP2009069137 W JP 2009069137W WO 2010100795 A1 WO2010100795 A1 WO 2010100795A1
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Prior art keywords
hollow shaft
photosynthesis
stirrer
carbon dioxide
light
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PCT/JP2009/069137
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English (en)
Japanese (ja)
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孝昭 前川
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株式会社筑波バイオテック研究所
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Priority to JP2011502592A priority Critical patent/JP5661028B2/ja
Publication of WO2010100795A1 publication Critical patent/WO2010100795A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED

Definitions

  • the present invention relates to a photosynthesis stirrer for efficiently producing a useful substance by utilizing the photosynthetic ability of aquatic organisms such as algae, a photobioreactor using the agitator, and a method for culturing aquatic organisms.
  • a stirrer type culture apparatus in which a light source for supplying light to the light emitting part is disposed outside the culture tank, and a device for supplying light and carbon dioxide into a culture tank containing a living organism having a photosynthetic ability and a culture solution.
  • a photosynthetic organism culture device (see Patent Document 2) that supplies light with a movable light emitter composed of a flat transparent material provided in the center of the culture tank, for receiving light from an external light source From a photosynthetic culture apparatus (see Patent Document 3) having an irradiation apparatus made of a plurality of independent plate-like light emitters connected to an optical transmission path in a culture tank (see Patent Document 3), a culture vessel, a carbon dioxide supply means, and a light irradiation means Composed
  • the culture vessel has a first zone for generating an upward flow of the culture solution as the bubbles generated by the supply of carbon dioxide and a second zone for generating a downward flow of the culture solution.
  • An apparatus for cultivating photosynthetic organisms that irradiates light (see Patent Document 4), a liquid film forming plate that causes a culture solution containing photosynthetic organisms to flow down into a liquid film along the surface, and a culture solution containing photosynthetic organisms at the upper end thereof
  • a culture medium supply means and a light source for irradiating the liquid film on the liquid film forming plate with light are provided, and a light reflecting surface is provided around the liquid film forming plate, and a light reflection surface
  • An apparatus for culturing photosynthetic organisms provided with a fiber exit end see Patent Document 5
  • a stirring blade disposed in the center of the culture tank containing the photosynthetic organisms and the culture solution and the rotation range of the stirring blade and the inner wall of the culture tank
  • Photosynthetic culture device with a plurality of plate-shaped light emitters (Patent text) 6), a rotation tank and a culture tank equipped with a field emission lamp fixed to the rotation axis.
  • the rotation axis is controlled to rotate the lamp around the rotation axis to stir the culture solution.
  • the light intensity and lighting of the lamp are controlled while acting as a stirring body to irradiate the medium with the required intensity of light, and the blinking timing of the lamp can be controlled to control the two light-dark reactions of the photosynthetic organism.
  • An optical bioreactor (see Patent Document 7) has been proposed.
  • a light emitting diode is also used as a light source, for example, a water tank having light reflectivity toward the inner surface, and a number of light emitting diodes arranged so that one end thereof is positioned above the culture liquid surface.
  • An apparatus for culturing microalgae equipped with stirring means is known (see Patent Document 8).
  • JP-A-3-58780 (Claims and others) JP-A-4-84883 (Claims and others) Japanese Patent Laid-Open No. 7-163331 (Claims and others) JP-A-7-184630 (Claims and others) JP-A-7-227269 (Claims and others) JP-A-7-289236 (Claims and others) JP 2008-283937 A (claims and others) JP-A-10-98964 (Claims and others)
  • the present invention is capable of uniformly dispersing and irradiating light necessary for a photosynthetic reaction in a culture solution without depending on sunlight, and without damaging aquatic organisms in culture by agitation.
  • An agitator for photosynthesis that can cultivate aquatic organisms efficiently while preventing the attachment of aquatic organisms to the wall, and that can extend the life of the light source, and photobio using the agitator.
  • the object of the present invention is to provide a reactor and a method for culturing aquatic organisms.
  • the present inventors have developed a light emitting diode that generates light having wavelengths of 430 nm, 680 nm, and 700 nm as a light source.
  • a light source, a cathode, a carbon dioxide supply pipe, and a feed pipe for nutrient solution, etc. are arranged at the lower part of the shaft part.
  • the present invention also serves as a carbon dioxide gas supply pipe, and a plurality of cylindrical or conical transparent stirring blades are attached to the hollow shaft constituting the rotating shaft in a radial manner and at different intervals, and the attachment
  • One or more light emitting diodes having three different light qualities of blue, red, and far red are provided in the vicinity of each part, and a nutrient solution or nutrient solution and an alkali solution for neutralizing carbon dioxide are provided below the hollow shaft.
  • a supply pipe for supplying a supply liquid including both of the above and a buffer mechanism for fixing an upward strut force generated by the supply of carbon dioxide gas and the supply liquid is fixed to the upper outer surface of the hollow shaft, Furthermore, the present invention provides a stirrer for photosynthesis characterized in that a power supply mechanism for a light emitting diode is slidably mounted on a hollow shaft.
  • the stirring blade is attached to the hollow shaft by changing the direction at the same angle in the range of 10 ° to 180 °, preferably 15 ° to 120 °. However, if the angle is reduced, it is advantageous that many stirring blades are attached.
  • one blue, one red and one far red light emitting diode must be used.
  • one blue and one far red light emitting diode can be used, and two red light emitting diodes can be used.
  • the range of the preferred red / blue light source intensity ratio (R / B ratio) is 4 to 15, and the range of the red / far red light source intensity ratio (R / FR ratio) is 1.3 to 2.5.
  • the present invention also provides a photobioreactor comprising a culture vessel in which the agitator is disposed.
  • the present invention provides the above-mentioned photosynthesis stirrer in which the photocatalyst for preventing the adhesion of underwater organisms is attached to the surface of the stirring blade is disposed in the culture solution, and is performed while stirring the culture solution.
  • a method for culturing organisms is provided.
  • a light emitting diode having three wavelength ranges is used as a light source, and light emitted from the light source is radiated in a direction perpendicular to the vessel wall surface through a cylindrical or conical transparent stirring blade.
  • the photosynthesis of the algae growing in the culture vessel is activated, the carbon dioxide fed in large quantities from the hollow shaft of the stirrer is used to photosynthesis very quickly, and the algae can be propagated in a short time.
  • the dry matter density of the algae in the culture vessel can be 5 to 15 times that of the conventional one.
  • algae can be produced at extremely low cost, and drying these algae can produce starch, lipids and proteins per unit area several hundred to several thousand times that of corn and sugarcane harvested from the field. become.
  • carbon dioxide with a mass approximately 1.5 times that of the dry matter density of algae is absorbed, so it becomes a carbon dioxide absorption device that is extremely effective in preventing global warming, and can be reused like a forest. Since carbon dioxide is not absorbed over several decades but is absorbed in a few days, it can be used as a device that always absorbs carbon dioxide regardless of location.
  • the dry matter of algae produced by the present invention is useful as it is for livestock feed, and has the advantage that the lipid contained in the dry matter can be used as fuel oil.
  • FIG. 1 is a longitudinal sectional view showing an example of a stirrer part of the present invention.
  • This stirrer part is divided and stored in an upper region and a lower region of a culture vessel, and is packed by an upper flange 9 and a lower flange 11. It is connected across.
  • the hollow shaft 2 constituting the rotating shaft of the stirrer also serves as the carbon dioxide supply pipe 1 and penetrates up and down through the entire culture vessel.
  • the upper end forms a carbon dioxide inlet and the lower end is a carbon dioxide outlet. It has become.
  • the lower end of the hollow shaft is supported via the holding portion 24 with a gap of 50 to 200 ⁇ m from the support member 14.
  • the support member 14 is fixed to the fixed bearing 15.
  • a supply pipe 16 is connected to the fixed bearing 15 to supply a supply liquid (hereinafter referred to as a supply liquid) containing a nutrient solution or both a nutrient solution and a carbon dioxide neutralizing alkaline solution into the lower end portion of the hollow shaft.
  • a supply liquid hereinafter referred to as a supply liquid
  • a cathode 5 is arranged in direct contact with the hollow shaft to form a cathode of a light emitting diode, and an insulator 4 is formed on the upper part.
  • the anode 3 is annularly connected without being in contact with the hollow shaft, and power is supplied to the light emitting diode through the anode 3.
  • a power supply mechanism for supplying power from the light source electrode to the upper stirrer driving portion and the stirrer A shock absorbing mechanism such as a mechanical spring or hydraulic means that generates a force acting in the downward direction against the thrust acting in the upward direction is attached, so that a certain amount of light energy can be supplied into the photobioreactor. It is like that.
  • a plurality of stirring blades 19 are attached to the hollow shaft 2 belonging to the lower stirrer via mounting cylinders 17.
  • the plurality of stirring blades 19 are cylindrical bodies or conical bodies, and are respectively attached at positions whose heights are changed radially at equal angles within a range of 10 ° to 180 °. This figure shows the case where the angle is 120 °. A0 ° B120 ° and C240 ° in the figure indicate the directions of the stirring blades.
  • the stirring blade 19 is preferably formed of a transparent material as a hollow body, and has three light emitting diodes 21, 21 ′, and 21 ′′ having blue, red, and far red light qualities in the vicinity of the mounting plate 18 (see FIG. (Not shown).
  • red / blue light source intensity ratio (R / B ratio) is adjusted to 4 to 15 by combining those having a light output of 0.25 to 10 mW with respect to the cross section of the stirrer.
  • the light source intensity ratio (R / FR ratio) of red / far red is preferably 1.3 to 2.5.
  • the light emitting diodes 21, 21 ′, 21 ′′ are attached to the hollow shaft 2 by cathode mounting screws 12,..., And an anode lead wire (not shown) for power supply is connected through the hole 13.
  • the hollow shaft 2 is held by a holding portion 24 at the lower end and is supported by a fixed bearing 15 via a support member 14.
  • the hollow shaft of this stirrer is made of a metal such as stainless steel, aluminum or copper, and the stirring blade is made of a light transmissive material such as transparent plastic or glass.
  • FIG. 2 is a sectional view showing an example of a buffer mechanism portion of the stirrer in the present invention, and shows an upper portion of the upper flange 9 of the hollow shaft 2 that also serves as the carbon dioxide supply pipe 1. .
  • a power supply mechanism including an anode 3, a cathode 5 of a light emitting diode, and an insulator 4 for maintaining an insulating state therebetween is provided so as to be slidable with respect to the hollow shaft.
  • a lead wire (not shown) is connected to the anode 3 via a fixture, and power is supplied from the power source to the light emitting diodes 21, 21 ', 21 ".
  • buoyancy or supply and supply of carbon dioxide by a gas filled in a space portion generated vertically upward in the stirrer, for example, a space portion accompanying installation of the light source is provided.
  • a buffer mechanism for generating a thrust downward is attached.
  • the shock-absorbing mechanism in this figure is composed of the spring 6, the thrust bearing 7 and the collar 8 that supports them, but other mechanical impacting means, hydraulic means, etc. can be used.
  • FIG. 3 is a cross-sectional view showing an example of the structure of the bottom of the stirrer in the present invention in more detail.
  • a holding part 24 is provided so as to be joined to the lower end 2 ′ of the hollow shaft of the stirrer.
  • the holding part 24 has a space serving as a gas-liquid mixing part 26 and faces the fixed bearing 15, and is 50 to 200 ⁇ m. It is supported by the support member 14 with a certain gap.
  • a check valve 25 is attached to the upper part of the holding part 24 to prevent liquid from entering the hollow shaft when the supply of carbon dioxide or the supply liquid is stopped.
  • the part of the support member 14 that is opposed to the lower end of the hollow shaft is constituted by the porous portion 23, and the supply liquid is supplied into the gas-liquid mixing portion 26 inside the hollow shaft 2 through this porous portion.
  • the fixed bearing 15 has a plenum chamber 22 to which a supply liquid supply pipe 16 is connected. The supply liquid is mixed in the gas-liquid mixing section 26 with the carbon dioxide sent from the carbon dioxide supply pipe 1 through the porous section 23.
  • the photosynthesis stirrer of the present invention when the aquatic organism, for example, algae, adheres to the transparent rotating blade portion close to the light source or the vessel wall surface that receives light from the light source of the culture vessel in which it is disposed, the light amount decreases, Therefore, it is preferable to apply a photo-oxidation catalyst to these surfaces. In this way, the growth rate of aquatic organisms can be increased, the dry matter density can be improved in a short time, and a dry matter density 5 to 15 times that of conventional photobioreactors can be obtained.
  • the photocatalyst used at this time platinum-supported titanium oxide is preferable, but other photocatalysts can also be used.
  • the production rate is controlled by the supply amount of carbon dioxide.
  • the alkaline liquid is supplied from below the hollow shaft 2, neutralizing carbon dioxide, and reducing the gas volume, so that a large amount of carbon dioxide can be supplied, The production efficiency of aquatic organisms can be significantly increased.
  • the light source mounting base 20 to which the light source is mounted is known. Can be reacted with carbon dioxide cooled in advance and maintained at room temperature. In this way, the light source replacement time can be increased by 5 to 10 times by maintaining the light source lifetime below 30% of the theoretical value.
  • the carbon dioxide used in the present invention is not necessarily pure, and may be one in which oxygen, nitrogen or other inert gas is mixed as long as the required photosynthesis reaction is not inhibited.
  • the alkaline solution include aqueous solutions of water-soluble alkali compounds such as sodium hydroxide, potassium hydroxide, sodium carbonate, potassium carbonate, sodium hydrogen carbonate, and potassium hydrogen carbonate. Used as nutrient solutions in cultures, eg, nitrates, hydrochlorides, sulfates, phosphates, etc. of metals such as sodium, potassium, magnesium, calcium, manganese, zinc, iron, cobalt Is used.
  • the concentration of these liquids is not particularly limited, and can be arbitrarily selected within the range conventionally used for culturing ordinary photosynthesis reactions.
  • the size of the photosynthesis stirrer of the present invention depends on the size of the culture vessel used.
  • the hollow shaft of the stirrer has an inner diameter of 10 to 200 mm, a length of 150 to 2500 mm, a wall thickness of 2 to 25 mm, The ratio of diameter to length is selected from the range of 1: 5 to 1:50, preferably 1:10 to 1:20.
  • the material for the photosynthesis stirrer is a transparent part, that is, the part of the stirring blade 19 and its mounting cylinder 17 is a transparent material such as a transparent hard plastic such as acrylic resin, polycarbonate, ABS resin, polyvinyl chloride, polyester, or the like. It is necessary to use glass, but other than that, it can be arbitrarily selected from materials commonly used in ordinary chemical equipment, such as metal, plastic, and wood.
  • culture conditions such as temperature, pressure, carbon dioxide supply rate, supply liquid supply rate, etc. can be used as they are in the conventional photosynthesis reaction by aquatic organisms.
  • aquatic organisms examples include green algae such as Chlorella, Chlamydomonas, Potricococcus oocystis, and cyanobacteria such as Spirulina, Anabena, Oshiratria, Formidium, and Nostock. Can be mentioned.
  • stirrer for photosynthesis used in the examples is a prototype shown in FIG. 1 in which the product name “MD-501” manufactured by Maruhishi Bioengineer Co., Ltd. is improved by Tsukuba Biotech Laboratories.
  • the spirulina was filtered and the first culture was discarded, and the total amount of spirulina filtered again was added to 4 liters of the culture that had been doubled, and the carbon dioxide gas supply rate was increased to 40 mL / min.
  • the dry matter concentration reached 10 ⁇ 1 g / L.
  • the culture solution formulation was quadrupled, the entire amount of Spirulina filtered by the same method was added, and the carbon dioxide gas supply rate was 80 mL / min, and the treatment was performed for 24 hours, and 15 g ⁇ 1 g / L was obtained in 75 hours.
  • the culture is continued for 24 hours at a rotation speed of 5 revolutions per minute, and the culture solution is continuously supplied from the supply pipe at a rate of 1 mL / min with a metering pump, and is pulled out at a level of 4 liters for HRT 2.8 hours continuous culture. Switching was continued for 10 days. Thereafter, the adhesion of the algae on the surface of the stirring blade glass surface and the inside of the glass wall inside the bioreactor was examined, and no adhesion of the algae wall surface was observed.
  • Example 2 Into a 5 L glass container, 4 liters of the culture solution was added, 1 g of chlorella was added thereto, and the mixture was supplied to the photosynthesis stirrer used in Example 1 at a rate of 20% / minute of 100% concentration carbon dioxide gas.
  • This stirrer had a circular stirrer blade with three glass tubes each having three light-emitting diodes each having a wavelength of 430 nm, 680 nm, and 700 nm disposed in advance.
  • the light reflecting material was spirally applied in an area of 1/3.
  • two light source mounting bases with three pairs of light emitting diodes fixed outside the photobioreactor were installed to create a light environment adjusted to have an R / B ratio of 5.
  • a photocatalyst supporting platinum on titanium oxide was mixed and applied to the glass surface of the stirring blade, and further applied to the inside of the glass wall of the photobioreactor.
  • a nutrient solution obtained by diluting the culture solution twice with distilled water is continuously supplied from the supply pipe at a rate of 1 mL / min with a metering pump, and pulled out. The operation was switched to continuous culture for 8 days and operated continuously for 10 days.
  • the dry matter concentration after 36 hours was 12 ⁇ 1 g / L, and stable production was achieved.
  • the power source was changed to a pulse wave, and production was carried out for 10 days in a light environment with a cycle of 0.2 seconds and a duty ratio of 50%, but the dry matter concentration was hardly changed.
  • light irradiation from the outer wall of the reactor was effective for algae production, and it was clarified that the decrease in yield was not observed even by pulse wave irradiation, leading to energy saving of the power source.
  • a power supply mechanism that supplies power to the light source power source as shown in FIG. 2 and a spring (mechanical coil spring) that generates a downward force opposite to the thrust acting upward of the stirrer are disposed above the thrust bearing.
  • 4 L of water was placed in a 5 L photobioreactor equipped with a photosynthesis stirrer used in Example 1, and 1 mL of 1% strength potassium hydroxide aqueous solution was added from the porous portion of the support member installed at the bottom of the reactor. / Min.
  • a labyrinth packing is attached to the upper part of the stirrer, and a mechanical system installed at the upper part is operated at a rotational speed of 5 revolutions while supplying a pressure of 0.05 MPa and 100% carbon dioxide gas at a rate of 40 mL / min.
  • a pressure of 0.05 MPa and 100% carbon dioxide gas at a rate of 40 mL / min.
  • a 1% strength aqueous potassium hydroxide solution was supplied at a rate of 1 mL / min from the porous portion of the support member installed in the lower part of the photobioreactor, and the pressure was 0.05 MPa, 100 from the upper part of the stirrer. While supplying carbon dioxide gas with a concentration of 40% / min, it was operated at a rotation speed of 5 rpm, and a pressure of 0.05 MPa and 100% carbon dioxide gas were supplied from the top of the stirrer at a rate of 40 mL / min. .
  • Example 1 Used in Example 1 in which a power supply mechanism and a spring (mechanical coil spring) as shown in FIG. 2 are disposed on the upper part of a thrust bearing in a 200 liter volume donut-shaped closed solar cell culture device having a depth of 18 cm.
  • One stirrer for photosynthesis was installed, and a 1% strength potassium hydroxide aqueous solution was added at a rate of 1 mL / min from the porous part of the support member installed at the lower part of the stirrer, and the pressure was 0.05 MPa, 100% from the upper part of the stirrer.
  • the photosynthesis stirrer of the present invention can perform a rapid photosynthesis reaction, it can mass-produce aquatic organisms such as algae, and is useful for the production of livestock feed and fuel oil. Moreover, since this photosynthesis can absorb a large amount of carbon dioxide of about 1.5 times the dry mass of algae, it is also useful as a carbon dioxide absorber.

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Abstract

Cette invention concerne un système de brassage pour photosynthèse au moyen duquel la lumière nécessaire pour obtenir une réaction photosynthétique peut éclairer de manière uniforme un milieu de culture liquide et diffuser dans le milieu sans dépendre de la lumière du soleil, des organismes aquatiques peuvent être cultivés en étant protégés des éventuels dommages causés par le brassage et en évitant leur adhésion à la paroi de la cuve, et la durée de vie de la source lumineuse peut être prolongée. Le système de brassage de l'invention comprend : un axe tubulaire (2) qui sert également de conduit d'approvisionnement en dioxyde de carbone (1) et qui constitue un axe rotatif; plusieurs lames de brassage transparentes cylindriques ou coniques (19) fixées de manière radiale à intervalles réguliers et à hauteurs irrégulières à l'axe tubulaire; une ou plusieurs diodes électroluminescentes (21) comportant trois différents types de lumière, notamment les lumières bleue, rouge et infrarouge, sont prévues à proximité de chacun des sites de fixation; un conduit d'acheminement d'une solution d'approvisionnement constituée d'une solution salée nutritive éventuellement accompagnée d'une solution alcaline pour neutraliser le dioxyde de carbone, est installé au pied de l'axe tubulaire; un mécanisme d'amortissement d'une force d'impulsion exercée vers le haut, qui est générée par l'apport du dioxyde de carbone et ladite solution d'approvisionnement, est fixé sur la face externe de la partie supérieure de l'axe tubulaire; et un mécanisme coulissant approvisionnant les diodes électroluminescentes en énergie électrique est fixé autour de l'axe tubulaire.
PCT/JP2009/069137 2009-03-04 2009-11-10 Système de brassage pour photosynthèse, photobioréacteur utilisant ce système de brassage et procédé de culture d'organismes aquatiques utilisant ce photobioréacteur WO2010100795A1 (fr)

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Cited By (4)

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JP2014509870A (ja) * 2011-03-29 2014-04-24 スレインテ アルガ テオランタ 微生物の生育
CN106430063A (zh) * 2016-11-24 2017-02-22 宣威市农硕农特产品开发有限公司 一种组培液用自动定量灌装系统
JP2019017263A (ja) * 2017-07-12 2019-02-07 エイブル株式会社 培養容器及び培養装置
KR102274961B1 (ko) * 2020-08-11 2021-07-07 독일에프에이유에를랑겐유체역학연구소 부산지사 3d 프린팅 기반 유체출입용 커넥터 및 그의 제조방법

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JP2014509870A (ja) * 2011-03-29 2014-04-24 スレインテ アルガ テオランタ 微生物の生育
CN106430063A (zh) * 2016-11-24 2017-02-22 宣威市农硕农特产品开发有限公司 一种组培液用自动定量灌装系统
CN106430063B (zh) * 2016-11-24 2018-04-03 宣威市农硕农特产品开发有限公司 一种组培液用自动定量灌装系统
JP2019017263A (ja) * 2017-07-12 2019-02-07 エイブル株式会社 培養容器及び培養装置
KR102274961B1 (ko) * 2020-08-11 2021-07-07 독일에프에이유에를랑겐유체역학연구소 부산지사 3d 프린팅 기반 유체출입용 커넥터 및 그의 제조방법

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