WO2010087628A3 - 유전자 발현을 증대시키는 신규한 핵산분자 및 이를 이용한 단백질 생산방법 - Google Patents

유전자 발현을 증대시키는 신규한 핵산분자 및 이를 이용한 단백질 생산방법 Download PDF

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Publication number
WO2010087628A3
WO2010087628A3 PCT/KR2010/000523 KR2010000523W WO2010087628A3 WO 2010087628 A3 WO2010087628 A3 WO 2010087628A3 KR 2010000523 W KR2010000523 W KR 2010000523W WO 2010087628 A3 WO2010087628 A3 WO 2010087628A3
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Prior art keywords
nucleic acid
acid molecules
gene expression
same
gene
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PCT/KR2010/000523
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English (en)
French (fr)
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WO2010087628A2 (ko
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최수근
박승환
정다은
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한국생명공학연구원
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Publication of WO2010087628A2 publication Critical patent/WO2010087628A2/ko
Publication of WO2010087628A3 publication Critical patent/WO2010087628A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 유전자 발현을 증대시키는 신규한 stab 핵산분자에 관한 것으로서, 상기 stab 핵산분자는 프로모터와 목적유전자 사이에 포함됨으로써 다운스트림 (downstream) 목적유전자의 발현율을 증대시킬 수 있다. 보다 구체적으로, 본 발명은 브레비바실러스 브레비스 (Brevibacillus brevis)의 mwp 유전자의 업스트림 (upstream)에 존재하는 신규한 stab 핵산분자, 및 상기 핵산분자의 3' 말단에 mwp 구조유전자의 5' 말단의 핵산분자가 연결된 신규한 stab핵산분자에 관한 것이다. 또한, 본 발명은 상기 stab 핵산분자를 포함하는 벡터, 상기 벡터를 포함하는 숙주세포, 및 상기 숙주세포로부터 목적 단백질을 생산하는 방법에 관한 것이다.
PCT/KR2010/000523 2009-01-30 2010-01-28 유전자 발현을 증대시키는 신규한 핵산분자 및 이를 이용한 단백질 생산방법 WO2010087628A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2009-0007587 2009-01-30
KR20090007587 2009-01-30

Publications (2)

Publication Number Publication Date
WO2010087628A2 WO2010087628A2 (ko) 2010-08-05
WO2010087628A3 true WO2010087628A3 (ko) 2010-12-09

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PCT/KR2010/000523 WO2010087628A2 (ko) 2009-01-30 2010-01-28 유전자 발현을 증대시키는 신규한 핵산분자 및 이를 이용한 단백질 생산방법

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KR (4) KR101416150B1 (ko)
WO (1) WO2010087628A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101280503B1 (ko) * 2010-02-03 2013-07-02 한국생명공학연구원 프로모터 변이체 및 이를 이용한 단백질 생산방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030027028A (ko) * 2000-08-14 2003-04-03 더 리전트 오브 더 유니버시티 오브 캘리포니아 개선된 살충성 박테리아, 및 그의 제조 및 이용 방법

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030027028A (ko) * 2000-08-14 2003-04-03 더 리전트 오브 더 유니버시티 오브 캘리포니아 개선된 살충성 박테리아, 및 그의 제조 및 이용 방법

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GUADALUPE PENA ET AL.: "A Bacillus thuringiensis S-Layer Protein Involved in Toxicity against Epilachna varivestis (Coleoptera: Coccinellidae)", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 72, no. 1, January 2006 (2006-01-01), pages 353 - 360, XP002418125, DOI: doi:10.1128/AEM.72.1.353-360.2006 *
HYUN-WOO PARK ET AL.: "Molecular Genetic Manipulation of Truncated Cry1C Protein Synthesis in Bacillus thuringiensis To Improve Stability and Yield", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 10, October 2000 (2000-10-01), pages 4449 - 4455, XP002458570, DOI: doi:10.1128/AEM.66.10.4449-4455.2000 *
JUAN MIRANDA-RIOS ET AL.: "A conserved RNA structure (thi box) is involved in regulation of thiamin biosynthetic gene expression in bactera", PNAS, vol. 98, no. 17, 2001, pages 9736 - 9741, XP002982411, DOI: doi:10.1073/pnas.161168098 *

Also Published As

Publication number Publication date
KR101416150B1 (ko) 2014-07-09
KR20100088557A (ko) 2010-08-09
KR101416148B1 (ko) 2014-07-09
KR20100088558A (ko) 2010-08-09
KR20100088559A (ko) 2010-08-09
KR20100088556A (ko) 2010-08-09
WO2010087628A2 (ko) 2010-08-05
KR101261845B1 (ko) 2013-05-07
KR101453437B1 (ko) 2014-10-21

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