WO2010083956A3 - Begasungssystem - Google Patents

Begasungssystem Download PDF

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Publication number
WO2010083956A3
WO2010083956A3 PCT/EP2010/000124 EP2010000124W WO2010083956A3 WO 2010083956 A3 WO2010083956 A3 WO 2010083956A3 EP 2010000124 W EP2010000124 W EP 2010000124W WO 2010083956 A3 WO2010083956 A3 WO 2010083956A3
Authority
WO
WIPO (PCT)
Prior art keywords
vector
gas
medium
bubble column
gassing
Prior art date
Application number
PCT/EP2010/000124
Other languages
English (en)
French (fr)
Other versions
WO2010083956A2 (de
Inventor
Joerg Kauling
Björn FRAHM
Andre Pütz
Markus Schindler
Original Assignee
Bayer Technology Services Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Technology Services Gmbh filed Critical Bayer Technology Services Gmbh
Priority to EP10700704A priority Critical patent/EP2389430A2/de
Priority to US13/145,823 priority patent/US20120052578A1/en
Publication of WO2010083956A2 publication Critical patent/WO2010083956A2/de
Publication of WO2010083956A3 publication Critical patent/WO2010083956A3/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/12—Cyclic peptides, e.g. bacitracins; Polymyxins; Gramicidins S, C; Tyrocidins A, B or C
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/232—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using flow-mixing means for introducing the gases, e.g. baffles
    • B01F23/2323—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using flow-mixing means for introducing the gases, e.g. baffles by circulating the flow in guiding constructions or conduits
    • B01F23/23231—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using flow-mixing means for introducing the gases, e.g. baffles by circulating the flow in guiding constructions or conduits being at least partially immersed in the liquid, e.g. in a closed circuit
    • B01F23/232311—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using flow-mixing means for introducing the gases, e.g. baffles by circulating the flow in guiding constructions or conduits being at least partially immersed in the liquid, e.g. in a closed circuit the conduits being vertical draft pipes with a lower intake end and an upper exit end
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/233—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
    • B01F23/2331—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements characterised by the introduction of the gas along the axis of the stirrer or along the stirrer elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/86—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis co-operating with deflectors or baffles fixed to the receptacle
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/90—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms 
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/10—Mixers with shaking, oscillating, or vibrating mechanisms with a mixing receptacle rotating alternately in opposite directions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Sustainable Development (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Gegenstand der Erfindung ist ein neuartiges Begasungssystem, das insbesondere aber nicht ausschließlich in der Biotechnologie zur Versorgung von Zellen oder Mikroorganismen mit Sauerstoff einsetzbar ist. Das Begasungssystem umfasst eine Blasensäule (40) und einen Verteiler (10). In dem Gefäß (100) befindet sich ferner ein flüssiges Medium, das mit Gas versorgt werden soll. Es wird ein Vektor als Transportmittel für das Gas eingesetzt. Das Gas wird in die Blasensäule eingetragen und hier von dem Vektor aufgenommen. Der Vektor wird in Tropfenform über den Verteiler auf die Flüssigkeitsoberfläche aufgetragen, sinkt in dem Medium nach unten und gibt einen Teil des aufgenommenen Gases an das Medium ab. Am Boden des Gefäßes befindet sich eine Sammel Vorrichtung, in der die Vektortropfen koaleszieren und wieder in die Blasensäule gelangen. Gegenstand der Erfindung ist weiterhin ein Bioreaktor umfassend das neuartige Begasungssystem. Gegenstand der Erfindung ist ferner ein Verfahren zur Begasung eines flüssigen Mediums, vorzugsweise einer Zellen oder Mikroorganismen enthaltenden wässrigen Suspension.
PCT/EP2010/000124 2009-01-23 2010-01-13 Begasungssystem WO2010083956A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP10700704A EP2389430A2 (de) 2009-01-23 2010-01-13 Begasungssystem
US13/145,823 US20120052578A1 (en) 2009-01-23 2010-01-13 Gassing system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009005962.8 2009-01-23
DE102009005962A DE102009005962A1 (de) 2009-01-23 2009-01-23 Begasungssystem

Publications (2)

Publication Number Publication Date
WO2010083956A2 WO2010083956A2 (de) 2010-07-29
WO2010083956A3 true WO2010083956A3 (de) 2011-03-03

Family

ID=41818788

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2010/000124 WO2010083956A2 (de) 2009-01-23 2010-01-13 Begasungssystem

Country Status (4)

Country Link
US (1) US20120052578A1 (de)
EP (1) EP2389430A2 (de)
DE (1) DE102009005962A1 (de)
WO (1) WO2010083956A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5627782B2 (ja) * 2010-07-30 2014-11-19 イー・エム・デイー・ミリポア・コーポレイシヨン 使い捨て渦防止装置
EP3294863A4 (de) * 2015-05-14 2019-01-16 Merck Sharp & Dohme Corp. In vitro
US9617191B1 (en) * 2016-06-09 2017-04-11 Xianggen Wu Bioreactor system and method
CN106732308A (zh) * 2017-01-13 2017-05-31 浙江大学 一种微鼓泡气液反应器
JP7173817B2 (ja) * 2018-10-10 2022-11-16 佐竹マルチミクス株式会社 炭酸ガス除去用の気泡発生部を有する培養装置
EP4051421A1 (de) * 2019-10-30 2022-09-07 Brammer Bio MA LLC Rührplattform zum aufrechterhalten der homogenität von lösungen
EP4324905A1 (de) * 2022-08-19 2024-02-21 Sartorius Stedim Biotech GmbH Bioreaktorsystem mit begasungsanlage

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001530A1 (en) * 1992-07-07 1994-01-20 Public Health Laboratory Service Board Process for modulating the concentration of dissolved gases in liquid media
EP1479758A2 (de) * 2003-04-30 2004-11-24 John Crane UK Limited Vorrichtung zum Begasen, vorzugsweise für die mikrobiologische Fermentation und Kultivierung von Zellen
DE102006022306A1 (de) * 2006-05-11 2007-11-15 Sartorius Biotech Gmbh Vibrationsmischer

Family Cites Families (19)

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Publication number Priority date Publication date Assignee Title
SE8306190L (sv) 1983-11-10 1985-05-11 Ivar Wergeland Forfarande och anordning for forbettrad syrsettning av biologiska kulturer
EP0258795B1 (de) 1986-08-27 1993-11-03 Kawasumi Laboratories, Inc. Verfahren und Vorrichtung zur Kultivierung von Zellen
JPH05219834A (ja) 1992-02-14 1993-08-31 Fumiko Kobayashi ディスポーサブル培養袋及び培養方法
IL119310A (en) 1996-09-26 1999-07-14 Metabogal Ltd Cell/tissue culturing device and method
US20050032211A1 (en) 1996-09-26 2005-02-10 Metabogal Ltd. Cell/tissue culturing device, system and method
US6190913B1 (en) 1997-08-12 2001-02-20 Vijay Singh Method for culturing cells using wave-induced agitation
US6550747B2 (en) 1998-10-09 2003-04-22 Zenon Environmental Inc. Cyclic aeration system for submerged membrane modules
US6432698B1 (en) 1999-01-06 2002-08-13 Rutgers, The State University Disposable bioreactor for culturing microorganisms and cells
CH697035A5 (de) 1999-05-04 2008-03-31 Marcel Roell Bioreaktor.
US6544788B2 (en) 2001-02-15 2003-04-08 Vijay Singh Disposable perfusion bioreactor for cell culture
US7897390B2 (en) 2003-11-18 2011-03-01 Nestec S.A. Cell culture system
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DE102006018824A1 (de) 2006-04-22 2007-10-25 Bayer Technology Services Gmbh Einweg-Bioreaktor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994001530A1 (en) * 1992-07-07 1994-01-20 Public Health Laboratory Service Board Process for modulating the concentration of dissolved gases in liquid media
EP1479758A2 (de) * 2003-04-30 2004-11-24 John Crane UK Limited Vorrichtung zum Begasen, vorzugsweise für die mikrobiologische Fermentation und Kultivierung von Zellen
DE102006022306A1 (de) * 2006-05-11 2007-11-15 Sartorius Biotech Gmbh Vibrationsmischer

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SHIRU JIA ET AL.: "Enhancement of Yeast Fermentation by addition of Oxygen Vectors in Air-Lift Bioreactor", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 84, no. 2, 1997, pages 176 - 178, XP002614445 *

Also Published As

Publication number Publication date
WO2010083956A2 (de) 2010-07-29
DE102009005962A1 (de) 2010-07-29
EP2389430A2 (de) 2011-11-30
US20120052578A1 (en) 2012-03-01

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