WO2010079919A3 - 다층기질겔을 이용한 기질분해효소의 스크리닝 방법 - Google Patents

다층기질겔을 이용한 기질분해효소의 스크리닝 방법 Download PDF

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Publication number
WO2010079919A3
WO2010079919A3 PCT/KR2009/007913 KR2009007913W WO2010079919A3 WO 2010079919 A3 WO2010079919 A3 WO 2010079919A3 KR 2009007913 W KR2009007913 W KR 2009007913W WO 2010079919 A3 WO2010079919 A3 WO 2010079919A3
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WIPO (PCT)
Prior art keywords
screening
gel
matrix metalloproteinases
sample
subjected
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PCT/KR2009/007913
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English (en)
French (fr)
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WO2010079919A2 (ko
Inventor
송재준
최종현
김보혜
최낙식
한윤전
이혜원
이승구
김중수
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한국생명공학연구원
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Publication of WO2010079919A2 publication Critical patent/WO2010079919A2/ko
Publication of WO2010079919A3 publication Critical patent/WO2010079919A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 2이상의 서로 다른 기질분해효소의 스크리닝의 스크리닝 및 활성검출방법에 관한 것으로, 보다 상세하게는 효소를 포함하는 시료를 겔 전기영동을 수행한 다음, 서로 다른 기질을 가지는 다층기질겔로 전기이동시켜 다층기질겔을 분석함으로써 2이상의 기질분해효소를 동시에 스크리닝하고 활성을 검출해내는것을 특징으로 하는 방법에 관한 것이다. 본 발명에 따른 기질분해효소의 스크리닝 및 효소활성 검출방법은 기존의 방법과 달리 하나의 겔에 시료를 로딩함으로써 동시에 2이상의 기질분해 효소의 스크리닝이가능하게 하는 장점이 있다. 또한, 미생물이나 환경소스로부터 미지의 효소를 직접 스크리닝하는 것을 가속화시키는 장점이 있으므로 유용하다.
PCT/KR2009/007913 2009-01-06 2009-12-29 다층기질겔을 이용한 기질분해효소의 스크리닝 방법 WO2010079919A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090000919A KR101062828B1 (ko) 2009-01-06 2009-01-06 다층기질겔을 이용한 기질분해효소의 스크리닝 방법
KR10-2009-0000919 2009-01-06

Publications (2)

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WO2010079919A2 WO2010079919A2 (ko) 2010-07-15
WO2010079919A3 true WO2010079919A3 (ko) 2010-10-21

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PCT/KR2009/007913 WO2010079919A2 (ko) 2009-01-06 2009-12-29 다층기질겔을 이용한 기질분해효소의 스크리닝 방법

Country Status (2)

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KR (1) KR101062828B1 (ko)
WO (1) WO2010079919A2 (ko)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002223792A (ja) * 2001-01-31 2002-08-13 Nippon Hikaku Kenkyusho リアルタイムザイモグラフィーによる酵素およびその阻害物質の検出・活性測定方法
WO2004019025A1 (ja) * 2002-08-21 2004-03-04 Toyo Kohan Co., Ltd. 固体支持体及び該固体支持体上に固定化された複数の物質又は複合体を脱離／イオン化することにより質量分析する方法
KR20050021146A (ko) * 2003-08-26 2005-03-07 김승 동충하초 추출물 유래의 혈전용해 효소
WO2005080960A1 (ja) * 2004-02-23 2005-09-01 Toyo Kohan Co., Ltd. 固体支持体及び該固体支持体上に複数の物質又は複合体を脱離／イオン化することにより質量分析する方法
WO2006041130A1 (ja) * 2004-10-14 2006-04-20 National University Corporation Hokkaido University ザイモグラフィー用ゲル

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002223792A (ja) * 2001-01-31 2002-08-13 Nippon Hikaku Kenkyusho リアルタイムザイモグラフィーによる酵素およびその阻害物質の検出・活性測定方法
WO2004019025A1 (ja) * 2002-08-21 2004-03-04 Toyo Kohan Co., Ltd. 固体支持体及び該固体支持体上に固定化された複数の物質又は複合体を脱離／イオン化することにより質量分析する方法
KR20050021146A (ko) * 2003-08-26 2005-03-07 김승 동충하초 추출물 유래의 혈전용해 효소
WO2005080960A1 (ja) * 2004-02-23 2005-09-01 Toyo Kohan Co., Ltd. 固体支持体及び該固体支持体上に複数の物質又は複合体を脱離／イオン化することにより質量分析する方法
WO2006041130A1 (ja) * 2004-10-14 2006-04-20 National University Corporation Hokkaido University ザイモグラフィー用ゲル

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KIM SH ET AL.: "Fibrin zymography: a direct analysis offibrinolytic enzymes on gels", ANAL BIOCHEM., vol. 263, no. 1, 1 October 1998 (1998-10-01), pages 115 - 116 *
LEBER TM ET AL.: "Zymography: a single-step staining method for quantitation of proteolytic activity on substrate gels", ANAL BIOCHEM., vol. 249, no. 1, 15 June 1997 (1997-06-15), pages 24 - 28 *

Also Published As

Publication number Publication date
WO2010079919A2 (ko) 2010-07-15
KR20100081615A (ko) 2010-07-15
KR101062828B1 (ko) 2011-09-07

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