WO2010059656A1 - Hybrides de, et cultivars dérivés du cultivar de riz appelé « cl151 » - Google Patents

Hybrides de, et cultivars dérivés du cultivar de riz appelé « cl151 » Download PDF

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Publication number
WO2010059656A1
WO2010059656A1 PCT/US2009/064883 US2009064883W WO2010059656A1 WO 2010059656 A1 WO2010059656 A1 WO 2010059656A1 US 2009064883 W US2009064883 W US 2009064883W WO 2010059656 A1 WO2010059656 A1 WO 2010059656A1
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rice
plant
plants
herbicide
seed
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PCT/US2009/064883
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English (en)
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Steven D. Linscombe
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Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College
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Priority to MX2011005356A priority Critical patent/MX345887B/es
Priority to EP09828127A priority patent/EP2358193A4/fr
Priority to US13/129,860 priority patent/US8946528B2/en
Publication of WO2010059656A1 publication Critical patent/WO2010059656A1/fr
Priority to US14/558,763 priority patent/US9499834B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8278—Sulfonylurea
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/50—Culture of aquatic animals of shellfish
    • A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Definitions

  • D2j This invention pertains to hybrids of. and cuhivars derived from the rice cultivar designated 'CL151.'
  • Rice is an ancient agricultural crop, and remains one of the world's principal food crops. There are two cultivated species of rice: Oryza saliva /,. , the Asian rice, and O. glaberrima St ⁇ iui, the African rice. Oryza sativa L. constitutes virtually all of the world's cultivated rice and is the species grown in the United States. Three major rice producing regions exist in the United States: the Mississippi Delta (Arkansas, Mississippi, northeast Louisiana, southeast Missouri), the Gulf Coast (southwest Louisiana, southeast Texas); and the Central Valley of California. See generally United States Patent No. 6,91 1,589. [0004] Rice is a semiaquatic crop that benefits from flooded soil conditions during part or all of the growing season.
  • rice is typically grown on flooded soil to optimize grain yields.
  • Heavy clay soils or silt loam soils with hard pan layers about 30 cm below "the surface are typical rice-producing soils, because they reduce water loss from soil percolation.
  • Rice production in the United States can be broadly categorized as either dry- seeded or water-seeded. In the dry-seeded system, rice is sown into a well-prepared seed bed with a grain drill or by broadcasting the seed and incorporating it with a disk or harrow. Moisture for seed germination comes from irrigation or rainfall. Another method of dry- seeding is to broadcast the seed by airplane into a flooded field, and then to promptly drain the water from the field.
  • One method of water-seeding is to soak rice seed for 12 to 36 hours to initiate germination, and then to broadcast the seed by airplane into a flooded field.
  • the seedlings emerge through a shallow flood, or the water may be drained from the field for a short period of time to enhance seedling establishment.
  • a shallow flood is then maintained until the rice approaches maturity.
  • the fields are drained when the crop is mature, and the rice is harvested. 2 to 3 weeks later with large combines.
  • Plant breeding begins with the analysis and definition of problems and weaknesses of the current germplasm, the establishment of program goals, and the definition of specific breeding objectives.
  • the next step is selection (or generation) of germplasm that possess the traits to meet the program goals.
  • the goal is often to combine in a single variety an improved combination of desirable traits from two or more ancestral germplasm lines.
  • These traits may include such things as higher seed yield, resistance to disease or insects, better stems and roots, tolerance to low temperatures, and better agronomic characteristics or grain quality.
  • breeding and selection methods depends on the mode of plant reproduction, the heritability of the trait(s) being improved, and the type of seed that is used commercially (e.g., Fj hybrid, versus pure line or inbred cultivars). For highly heritable traits, a choice of superior individual plants evaluated at a single location may sometimes be effective, while for traits with low or more complex heritability, selection is often based on mean values obtained from replicated evaluations of families of related plants. Selection methods include pedigree selection, modified pedigree selection, mass selection, recurrent selection, and combinations of these methods. f ⁇ Ollj The complexity of inheritance influences the choice of breeding method.
  • a particularly difficult task is the identification of individual plants that are, indeed, genetically superior.
  • a plant's phenotype results from a complex interaction of genetics and environment.
  • One method for identifying a genetically superior plant is to observe its performance relative to other experimental plants and to a widely grown standard culfivar raised in an identical environment. Repeated observations from multiple locations can help provide a better estimate of its genetic worth.
  • the goal of rice breeding is to develop new, unique, and superior rice c ⁇ ltivars and hybrids.
  • the breeder initially selects and crosses two or more parental lines, followed by self pollination and selection, producing many new genetic combinations.
  • the breeder can generate billions of different genetic combinations via crossing, selling, and mutation breeding.
  • the traditional breeder has no direct control at the molecular level. Therefore, two traditional breeders working independently of one another will never develop the same line, or even very similar lines, with the same traits.
  • Hybrid seed is typically produced by manual crosses between selected male-fertile parents or by using genetic male sterility systems. These hybrids are typically selected for single gene traits that unambiguously indicate that a plant is indeed an Fi hybrid that has inherited traits from both presumptive parents, particularly the male parent (since rice normally self- fertilizes). Such traits might include, for example, a semi dwarf plant type, pubescence, awns, or apiculus color. Additional data on parental lines, as well as the phenotype of the hybrid, influence the breeder's decision whether to continue with a particular hybrid cross or an analogous cross, using related parental lines.
  • Pedigree breeding and recurrent selection breeding methods are sometimes used to develop cultivars from breeding populations. These breeding methods combine desirable traits from two or more cultivars or other germplasm sources into breeding pools from which cultivars are developed by selling and selection of desired phenotypes. The new cultivars are evaluated to determine commercial potential.
  • Pedigree breeding is often used to improve self-pollinating crops. Two parents possessing favorable, complementary traits are crossed to produce F-. plants. An F 2 population is produced by selling one or more Fis. Selection of the superior individual plants may begin in the F 2 (or later) generation. Then, beginning in the F 3 (or other subsequent) generation, individual plants are selected. Replicated testing of panicle rows from the selected plants can begin in the F4 (or other subsequent) generation, both to fix the desired traits and to improve the effectiveness of selection for traits that have low heritability. At an advanced stage of inbreeding (e.g., F 6 or F 7 ), the best lines or mixtures of phcnotypically- similar lines are tested for potential release as new cultivars.
  • F 6 or F 7 the best lines or mixtures of phcnotypically- similar lines are tested for potential release as new cultivars.
  • Mass and recurrent selection methods can also be used to improve populations of either self- or cross-pollinating crops.
  • a genetically variable population of heterozygous individuals is either identified or created by intercrossing several different parents. The best offspring plants are selected based on individual superiority, outstanding progeny, or excellent combining ability. The selected plants are intercrossed to produce a new population in which further cycles of selection are continued.
  • Backcross breeding is often used to transfer genes for a simply inherited, highly heritable trait into a desirable homozygous cultivar or inbred line, which is the recurrent parent.
  • the source of the trait to be transferred is called the donor parent.
  • the resulting plant should ideally have the attributes of the recurrent parent (e.g., cultivar) and the desired new trait transferred from the donor parent.
  • individuals possessing the desired donor phenotype e.g., disease resistance, insect resistance, herbicide tolerance
  • backcrosscd to the recurrent parent.
  • the single-seed descent procedure in the strict sense refers to planting a segregating population, harvesting a sample of one seed per plant, and using the one-seed sample to plant the next generation.
  • the plants from which lines are derived will each trace to different F?. individuals.
  • the number of plants in a population declines each generation due to failure of some seeds to germinate or some plants to produce at least one seed. As a result, not all of the F 2 plants originally sampled in the population will be represented by a progeny when generation advance is completed.
  • a multiple-seed procedure the breeder harvests one or more seeds from each plant in a population and threshes them together to form a bulk. Part of the bulk is used to plant the next generation and part is put in reserve.
  • the procedure has been referred to as modified single-seed descent or the pod-bulk technique.
  • the multiple-seed procedure has been used to save labor at harvest, ft is considerably faster to thresh panicles by machine than to remove one seed from each by hand as in the single-seed procedure.
  • the multiple- seed procedure also makes it possible to plant the same number of seeds from a population for each generation of inbreeding. Enough seeds are harvested to compensate for plants that did not germinate or produce seed.
  • Proper testing should detect any major faults and establish the level of superiority or improvement over current cultivars. In addition to showing superior performance, there must be a demand for a new cultivar or hybrid that is compatible with industry standards or that creates a new market. The introduction of a new cultivar or hybrid may incur additional costs to the seed producer, the grower, processor and consumer for such things as special advertising and marketing, altered seed and commercial production practices, and new product utilization. The testing preceding release of a new cultivar or hybrid should take into consideration research and development costs as well as technical superiority of the final cultivar or hybrid.
  • hybrids of, and cuitivars derived from, the herbicide- resistant, long-grain rice cuJtivar designated 'CL 151 a herbicide-tolerant eultivar that has superior lodging, processing, and grain yield characteristics.
  • This invention also pertains to methods for producing a hybrid or new variety by crossing the rice variety 'CL151' with another rice line, one or more times.
  • any such methods using the rice variety 'CL151' are aspects of this invention, including backcrossing, hybrid production, crosses to populations, and other breeding methods involving 'CL 151.
  • Hybrid plants produced using the rice variety 'CL151' as a parent are also within the scope of this invention.
  • either parent can, through routine manipulation of cytoplasmic or other factors through techniques known in the art. be produced in a male -sterile form.
  • this invention allows for single-gene converted plants of 'CLl 51.
  • the single transferred gene may be a dominant or recessive allele.
  • the single transferred gene confers a trait such as resistance to insects, one or more bacterial, fungal or viral diseases, male fertility or sterility, enhanced nutritional quality, enhanced processing qualities, or an additional source of herbicide resistance.
  • the single gene may be a naturally occurring rice gene or a transgene introduced through genetic engineering techniques known in the art. The single gene also may be introduced through traditional backcrossing techniques or genetic transformation techniques known in the art.
  • this invention provides r ⁇ generable cells for use in tissue culture of rice plant 'CL151 .
  • the tissue culture may allow for regeneration of plants having physiological and morphological characteristics of rice plant 'CL151' and of regenerating plants having substantially the same genotype as rice plant 'CI.151.
  • Tissue culture techniques for rice arc known in the art.
  • the rcgenerablc cells in tissue culture may be derived from sources such as embryos, protoplasts, meristematic cells, callus, pollen, leaves, anthers, root tips, flowers, seeds, panicles, or stems.
  • the invention provides rice plants regenerated from such tissue cultures. Th e following definitions apply throughout the specification and claims, unless context clearly indicates otherwise:
  • Grain yield is measured in pounds per acre, at 12.0% moisture.
  • Grain yield depends on a number of factors, including the number of panicles per unit area, the number of fertile florets per panicle, and grain weight per floret.
  • Lodging Percent Lodging is a subjectively measured rating, and is the percentage of plant stems leaning or fallen completely to the ground before harvest.
  • G Gram Length
  • 1000 Grain Wi The weight of 1000 rice grains, measured in grams.
  • Hardvest Moisture The percentage moisture in the grain when harvested.
  • Plant Height Plant height in centimeters, measured from soil surface to the tip of the extended panicle at harvest
  • amylose Percent The percentage of the endosperm starch of milled rice that is amylose. The apparent amylose percent is an important grain characteristic that affects cooking behavior. Standard long grains contain 20 to 23 percent amylose. Rexmont-type long grains contain 24 to 25 percent amylose. Short and medium grains contain 13 to 19 percent amylose. Waxy rice contains zero percent amylose. Amylose values, like most characteristics of rice, depend on the environment. "Apparent” refers to the procedure for determining amylose, which may also involve measuring some long chain amylop ⁇ ctin molecules that bind to some of the amylose molecules. These amylopectin molecules actually act similar to amylose in determining the relative hard or soft cooking characteristics.
  • Alkali Spreading Value An index that measures the extent of disintegration of the milled rice kernel when in contact with dilute alkali solution. An indicator of geiatinization temperature. Standard long grains have a 3 to 5 Alkali Spreading Value (intermediate geiatinization temperature).
  • Peak Viscosity The maximum viscosity attained during heating when a standardized, instrument-specific protocol is applied to a defined rice flour-water slurry.
  • Trough Viscosity The minimum viscosity after the peak, normally occurring when the sample starts to cool.
  • Setback 1 is the final viscosity minus the trough viscosity.
  • Setback 2 is the final viscosity minus the peak viscosity.
  • Viscosity As measured by a Rapid Visco Analyzer, is a new but widely used laboratory instrument to examine paste viscosity or thickening ability of milled rice during the cooking process.
  • Allele is any of one or more alternate forms of the same gene. In a diploid cell or organism, the two alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes.
  • Backcrossing is a process in which a breeder repeatedly crosses hybrid progeny back to one of the parents, for example, crossing a first generation hybrid Fi with one of the parental genotypes of the Fi hybrid, and then crossing a second generation hybrid F> with the same parental genotype, and so forth.
  • Quantitative Trait Loci Quantitative Trait Loci (QTL).” Quantitative trait loci (QTL) refer to genetic loci that to some degree control numerically measurable traits, generally traits that are continuously distributed.
  • Regeneration refers to the development of a plant from tissue culture.
  • Single Gene Converted (Conversion). Single gene converted (conversion) includes plants developed by backcrossing wherein essentially all of the desired morphological and physiological characteristics of a parental variety are recovered, while retaining a single gene transferred into the variety via crossing and backcrossing. The term can also refer to the introduction of a single gene through genetic engineering techniques known in the art,
  • 'CL 151 ' is a serai-dwarf, long-grain rice variety that contains, by common ancestry, the same gene for herbicide resistance as that found in the cultivar 'CL161 .
  • the pedigree for this line is CFX-26/9702128.
  • CFX-26 is an imazethapyr-resistant mutant derived from the variety Cypress. See U.S. Patent 7,019,196.
  • the 9702128 line (Lemont/20001-5/3/Leraont//L-202/TDCN) is an experimental line that was never released as a commercial variety.
  • 'CL 151 has averaged 101 cm in height, which is 8 cm shorter than 'CLl 61 ,' and 10 cm taller than 'CL13 L' It displays short awns very infrequently. Leaves are dark green, and display an intermediate leaf angle, 'CL151 ' is comparable in maturity than 'Cl-131 ,' and 4 days earlier in maturity than " CL.161.” It has a purple apiculus, a color that fades as the grains approach maturity. The lemma and palea are straw-colored and glabrous. The bran is light-brown. It is nonglutinous. The grain averages 22.4 aniylose, and has an intermediate gelatinization temperature.
  • the line is highly resistant to imidazolinone herbicides, including but not limited to imazethapyr and imazarnox, fts herbicide resistance characteristics are essentially identical to those of 'CL 161 " (ATCC deposit PTA-904).
  • 'CLl 51 " is susceptible to sheath blight, blast, and bacterial panicle blight.
  • 'CLl 51 " and its hybrids and derived varieties are adapted for growing throughout the rice growing areas of Louisiana, Texas, Arkansas. Mississippi and Missouri: and will also be well suited for growing in many other rice-producing areas throughout the world.
  • the line was harvested and selected through early generations for phcnotypic superiority for characteristics such as short plant architecture, grain shape and uniformity, seedling vigor, tiller number, and grain size. In later generations (seed increase), the line was selected for uniformity and purity both within and between panicle rows. Foundation seed rice was eventually grown beginning with the F 7 generation. Seed from the F 5 , F 6 , and F 7 generations was entered into an experimental line testing program, and were also tested at several locations in Louisiana rice producing areas. 10058] Across testing in several trials at multiple Louisiana locations during a three- year period, overall av erage grain yield was 7600 Ib A. for 'CL151 ,' compared to 6862 Ib-A for 'CL ⁇ 1 ' and 6816 Ib A for Tl 161/
  • CULM (Degrees from perpendicular after flowering
  • INSECT RESISTANCE Rice Water Weevil (Lissorhoptrus oryz ⁇ phiius): Susceptible
  • Tlic variety is resistant to imidazolinone herbicides.
  • the herbicide resistance profile is essentially the same as that of 'CLl 61 ,' being derived from common ancestry.
  • the herbicide tolerance allows 'CLl 51 5 ' its hybrids, and derived varieties to be used with ClearficldTM rice technology and herbicides, including among others imazcthapyr and imazamox, for the selective control of weeds, including red rice. See generally U.S. Patent 6,943,280.
  • 'CLl 51' The variety is tolerant to some herbicides, and susceptible to some herbicides, that normally inhibit the growth of rice plants.
  • the herbicide tolerance and susceptibility characteristics of 'CLl 51' include the following: "CLlS r expresses a mutant acelohydroxyacid synthase whose enzymatic activity is directly resistant to normally-inhibitory levels of a herbicidally- cffectiv c i mi dazol inone ;
  • 'CLl 51 ' is resistant to each of the following imidazoiinone herbicides, at levels of the i mi dazol inone herbicides that would normally inhibit the growth of a rice plant: imazethapyr, imazapic, imazaq ⁇ in, imazamox, and imazapyr;
  • 'CL151 ' is resistant to each of the following sulfonylurea herbicides, at levels of the sulfonylurea herbicides that would normally inhibit the growth of a rice plant: nicosulfuron, metsulfuron methyl, thifensulfuron methyl, and tribenuron methyl;
  • 'CL151 * is sensitive to each of the following sulfonylurea herbicides, at levels of the sulfonylurea herbicides that would normally inhibit the growth of a rice plant: sulfometuron methyl, chlorimuron ethyl, and rimsulfuron.
  • This invention is also directed to methods for producing a rice plant by crossing a first parent rice plant with a second parent rice plant, wherein the first or second rice plant is a rice plant from the line 'CL 151.
  • first and second parent rice plants may be from the cultivar 'CL151,' although it is preferred that one of the parents be different.
  • Methods using the cultivar 'CLl 5 V are part of this invention, including crossing, selling, backcrossing, hybrid breeding, crossing to populations, Ih ⁇ other breeding methods discussed earlier in this specification, and other breeding methods known to those of skill in the art. Any plants produced using cultivar 'CL151' as a parent or ancestor are within the scope of this invention.
  • the other parents or other lines used in such breeding programs may be any of the wide number of rice varieties, cultivars, populations, experimental lines, and other sources of rice gerraplasra known in the art.
  • this invention includes methods for producing a first-generation hybrid rice plant by crossing a first parent rice plant with a second parent rice plant, wherein either the first or second parent rice plant is 'CL 151.
  • this invention is also directed to methods for producing a hybrid rice line derived from 'CL151' by crossing 'CL151' with a second rice plant, and growing the progeny seed. The crossing and growing steps may be repeated any number of times. Breeding methods using the ric ⁇ line 'CLl 51 ' are considered part of this invention, not only backcrossing and hybrid production, but also selling, crosses to populations, and other breeding methods known in the art.
  • either of the parents in such a cross, 'CLl 51 ' or the other parent may be produced in male-sterile form, using techniques known in the art.
  • plant includes plant cells, plant protoplasts, plant cells of tissue culture from which rice plants can be regenerated, plant calli, plant clumps, and plant cells that are intact in plants or parts of plants, such as pollen, flowers, embryos, ovules, seeds, pods, leaves, stems, anthers and the like.
  • another aspect of this invention is to provide for cells that, upon growth and differentiation, produce a cultivar having essentially all of the physiological and morphological characteristics of 'CLl 51.'
  • Methods of introducing expression vectors into plant tissue include the direct infection or co-culdvation of plant cells with Agrobactcriurn tumefaciens, Horsch el ah, Science, 227: 1229 (1985). Descriptions of Agrobacterium vectors systems and methods for Agrobacterium- mediated gene transfer are provided by Gruber et ah, supra, [0083] Useful methods include but arc not limited to expression vectors introduced into plant tissues using a direct gene transfer method such as microproj ⁇ ctil ⁇ -mediated delivery, DNA injection, electroporation and the like. More preferably expression vectors are introduced into plant tissues using the microprojectil ⁇ media delivery with biolistic device- or Agrobacterium-medialed transformation. Transformed plants obtained with the germplasm of CLiS I' are intended to be within the scope of this invention.
  • the present invention also provides rice plants regenerated from a tissue culture of the 'CLl 51' variety or hybrid plant.
  • tissue culture can be used for the in vitro regeneration of a rice plant.
  • Chu, Q. R. et al. (1999) "Use of bridging parents with high anther culturability to improve plant regeneration and breeding value in rice," Rice Biotechnology Quarterly, 38:25-26: Chu. Q. R. et al, "A novel plant regeneration medium for rice anther culture of Southern U.S. crosses," Rice Biotechnology Quarterly, 35: 15-16 (1998); Chu, Q.
  • Another aspect of this invention is to provide cells that, upon growth and differentiation, produce rice plants having all or essentially all, of the physiological and morphological characteristics of variety 'CL 151.' [0085] Unless context clearly indicates otherwise, references in the specification and claims to 'CLl 51 ' should be understood also to include single gene conversions of 'CL 15 L' male sterility, other sources of herbicide resistance, resistance for bacterial, fungal, or viral disease, insect resistance, male fertility, enhanced nutritional quality, industrial usage, yield stability and yield enhancement.
  • Rao et al. Maize Genetics Cooperation Newsletter, 60:64-65 (1986), refers to somatic embryogenesis from glume callus cultures and B. V. Conger el al, Plant Cell Reports, 6:345-347 (1987) reported somatic embryogenesis from the tissue cultures of corn leaf segments. These methods of obtaining plants are routinely used with a high rate of success.
  • Corn tissue culture procedures which may be adapted for use with rice, are also described in Green et al., "Plant Regeneration in Tissue Culture of Maize," Mate for Biological Research (Plant Molecular Biology Association, Charlottesville, Va., pp. 367-372, 1982) and in Duncan el aL, "T3ie Production of Callus Capable of Plant Regeneration from Immature Embryos of Numerous Zea Mays Genotypes," 165 Planta, 322:332 (1985).
  • another aspect of this invention is to provide ceils that, upon growth and differentiation, produce rice plants having all, or essentially all, of the physiological and morphological characteristics of hybrid rice line 'CL15L' See T. P. Croughan ei aL, (Springer- Verlag, Berlin, 1991) Rice ⁇ Oryza saliva. L): Establishment of Callus Culture and the regeneration of Plants, in Biotechnology in Agriculture and Forestry (19-37).
  • transgenic ⁇ s are herein referred to collectively as "transgen ⁇ s.”
  • transgenic plants Over the last 15 to 20 years, several methods for producing transgenic plants have been developed, and the present invention, in particular embodiments, also relates to transformed versions of 'CLl 5 L' [0089] An expression vector is constructed that will function in plant cells.
  • Such a vector comprises a DNA coding sequence under the control of or operativeiy iinked to a regulatory element (e.g., a promoter).
  • the expression vector may contain one or more such operably linked coding sequence/regulatory element combinations.
  • the vector(s) may be in the form of a plasmid, and can be used alone or in combination with other plasmids to provide transformed rice plants.
  • Expression vectors commonly include at least one genetic "marker,” operably linked to a regulatory element (e.g.. a promoter) that allows transformed cells containing the marker to be either recovered by negative selection, i.e., inhibiting growth of cells that do not contain the selectable marker gene, or by positive selection, i.e., screening for the product encoded by the genetic marker.
  • a regulatory element e.g.. a promoter
  • Many commonly used selectable marker genes for plant transformation are known in the art, and include, for example, genes that code for enzymes that raetabolJcally detoxify a selective chemical inhibitor such as an antibiotic or a herbicide, or genes that encode an altered target that is insensitive to such an inhibitor. Positive selection methods are also known in the art.
  • a commonly used selectable marker gene for plant transformation is that for neomycin phosphotransferase TT (nptll), isolated from transposon Tn5, whose expression confers resistance to kanamycin. See Fraley et aL, Proc. Nail. Acad. Sd. U.S.A., 80:4803 (1983).
  • Another commonly used selectable marker gene is the hygromycin phosphotransferase gene, which confers resistance to the antibiotic hygromycin. See Vanden Elzen et aL, Plant MoL Biol., 5:299 (1985).
  • Additional selectable marker genes of bacterial origin that confer resistance to one or more antibiotics include gentamycin acetyl transferase, streptomycin phosphotransferase, aminoglycoside-3'-adenyl transferase, and the bleomycin resistance determinant. Hayford el aL, Plant Physiol., 86: 1216 (1988), Jones el aL, MoL Gen. Genet., 210:86 (1987), Svab et aL, Plant MoL Biol., 14: 197 (1990); Plant MoI. Biol., 7: 171 (1986).
  • selectable marker genes confer resistance to herbicides such as glyphosate, glufosinatc, or broxynil. Comai et aL, Nature, 317:741-744 (1985); Gordon-Kamm et aL, Plant Cell, 2:603-618 (1990); and Stalker s aL, Science, 242:419-423 (1988).
  • Selectable marker genes for plant transformation of non-bacterial origin include, for example, mouse dihydrofolate reductase, plant 5-enolpyruvylshikimate-3- phosphate synthase, and plant acetolactate synthase.
  • Eichholtz et aL Somatic Cell MoL Genet. 13:67 ( 1987); Shah et aL, Science, 233:478 (1986); and Charest et aL, Plant Cell Rep., 8:643 (1990).
  • marker genes for plant transformation employs screening of presumptively transformed plant cells, rather than selection for resistance to a toxic substance such as an antibiotic. These marker genes are particularly useful to quantify or visualize the spatial pattern of expression of a gene in specific tissues, and arc frequently referred to as reporter genes because they may be fused to the target gene or regulatory sequence. Commonly used reporter genes include glucuronidase (GUS), galaclosidase, luciferase, chloramphenicol, and acctyJtransfcrase. Sec Jefferson, R. A,, Plant MoL Biol.
  • GFP Green Fluorescent Protein
  • Genes included in expression vectors are driven by a nucleotide sequence comprising a regulatory element, for example, a promoter.
  • a regulatory element for example, a promoter.
  • Many suitable promoters are known in the art, as are other regulatory elements that may be used either alone or in combination with promoters.
  • promoter refers to a region of DNA upstream from the transcription initiation site, a region that is involved in recognition and binding of RNA polymerase and other proteins to initiate transcription.
  • a "plant promoter” is a promoter capable of initiating transcription in plant cells. Examples of promoters under developmental control include promoters that preferentially initiate transcription in certain tissues, such as leaves, roots, seeds, fibers, xylem vessels, tracheitis, or sclerenchyma.
  • tissue-preferred Promoters that initiate transcription only in certain tissue are referred to as "tissue-specific.”
  • a "cell type” specific promoter primarily drives expression in certain cell types in one or more organs, for example, vascular cells in roots or leaves.
  • An “inducible” promoter is a promoter that is under environmental control. Examples of environmental conditions that may induce transcription by inducible promoters include anaerobic conditions or the presence of light. Tissue- specific, tissue -preferred, cell type specific, and inducible promoters are examples of “non-constitutive” promoters.
  • a “constitutive” promoter is a promoter that is generally active under most environmental conditions.
  • an inducible promoter is operably linked to a gene for expression in rice.
  • the inducible promoter is operably linked to a nucleotide sequence encoding a signal sequence that is operably linked to a gene for expression in rice. With an inducible promoter the rate of transcription increases in response to an inducing agent.
  • Any suitable inducible promoter may be used in the present invention. See
  • a preferred inducible promoter is one that responds to an inducing agent to which plants do not normally respond, for example, the inducible promoter from a steroid hormone gene, the transcriptional activity of which is induced by a glucocorticosteroid hormone. See Schena et al, Proc. Nat L Acad Set., U.S.A. 88:0421 (1991 ).
  • a constitutive promoter is operably linked to a gene for expression in rice, or the constitutive promoter is operabiy linked to a nucleotide sequence encoding a signal sequence that is operably linked to a gene for expression in rice.
  • Constitutive promoters may also be used in the instant invention. Examples include promoters from plant viruses such as the 35S promoter from cauliflower mosaic virus, Odell et ai, Nature, 313:810-812 (1985), and the promoters from the rice actin gene, McElroy el al.. Plant Cell, 2: 163-171 (1990); ubiquitin, Christensen et ai, Plant MoI. Biol., 12:619-632 (1989) and Christensen et ai, Plant MoI. Biol. 18:675-689 (1992); pEMU, Last et al, Theor.
  • promoters from plant viruses such as the 35S promoter from cauliflower mosaic virus, Odell et ai, Nature, 313:810-812 (1985), and the promoters from the rice actin gene, McElroy el al.. Plant Cell, 2: 163-171 (1990); ubiquitin, Christensen et ai, Plant
  • AHAS ALS
  • Brassica napus ALS3 structural gene (or a nucleotide sequence similar to said Xbal/Ncol fragment), may be used as a constitutive promoter. See PCX Application VVO 96/30530. The promoter from a rice ALS (AHAS) gene may also be used. See the sequences disclosed in PCT Application WO 01 /85970; and U.S. Patent Mo. 6,943,280.
  • a tissue-specific promoter is operably linked to a gene for expression in rice.
  • tissue-specific promoter is operably linked to a nucleotide sequence encoding a signal sequence that is operably linked to a gene for expression in rice.
  • Transformed plants produce the expression product of the transgen ⁇ exclusively, or preferentially, in specific
  • tissue-specific or tissue-preferred promoter may be used in the instant invention.
  • tissue-specific or tissue-preferred promoters include those from the phaseolhi gene, Murai et al., Science, 23:476-482 (1983), and Sengupta-Gopalan et al., Proc. Natl. Acad. ScL U.S.A., 82:3320-3324 (1985); a leaf-specific and light-induced promoter such as thai from cab or rubisco, Simpson et al., EMBO J., 4(11):2723-2729 ( 1985) and Timko et al..
  • an anther-specific promoter such as that from LAT52, Tw ell et ai, MoI. Gen. Genetics, 217:240-245 (1989); a policn-specif ⁇ c promoter such as that from ZmI 3, Guerrero et al,, MoI. Gen, Genetics, 244: 161-168 (1993): or a microspor ⁇ -pref ⁇ rred promoter such as that from apg, Tw ell et ai, Sex. Plant Reproci, 6:217-224 (1993).
  • Transport of protein or peptide molecules produced by lransgenes to a subcellular compartment such as a chioroplast, vacuole, peroxisome, glyoxysornc, ceil wall, or mitochondrion, or for secretion into an apoplast is accomplished by opera bly linking a nucleotide sequence encoding a signal sequence to the 5' or 3' end of a gene encoding the protein or peptide of interest.
  • Targeting sequences at the 5' or 3' end of the structural gene may determine, during protein synthesis and processing, where the encoded protein is u 1 ti mately compartmen ta lized.
  • Agronomically significant genes that may be transformed into rice plants in accordance with the present invention include, for example, the following:
  • Plant disease resistance genes Plant defenses arc often activated by specific interaction between the product of a disease resistance gene (R) in the plant and the product of a corresponding avirulence (Avr) gene in the pathogen.
  • R disease resistance gene
  • Avr avirulence
  • a plant may be transformed with a cloned resistance gene to engineer plants that are resistant to specific pathogen strains. Sec, e.g., Jones et ah, Science 266:789 (1994) (cloning of the tomato Cf-9 gene for resistance to Cladosporiiim fulvum); Martin et ⁇ /.. Science 262: 1432 (1993) (tomato Pto gene for resistance to Pseudomonas syringae pv.
  • Tomato encodes a protein kinase
  • Mindrinos et ah Cell 78: 1089 (1994) (Arabidopsis RSP2 gene for resistance to Pseudomonas syringae).
  • B. A BaciHus thuringiensis protein, a derivative thereof, or a synthetic polypeptide modeled thereon. See, e.g., Geiser el ai. Gene 48: 109 (1986), disclosing the cloning and nucleotide sequence of a Bt-cndotoxin gene. DNA molecules encoding endotoxin genes may be obtained from American Type Culture Collection, Manassas, Va., e.g., under ATCC Accession Nos. 40098, 67136, 31995, and 31998.
  • a lectin See, for example. Van Damme et ai. Plant Molec, Biol, 24:25 (1994), disclosing the nucleotide sequences of several Clivia miniaia mannose-binding lectin genes.
  • a vitamin-binding protein such as avidin. See PCT Application US93/06487. This disclosure teaches the use of avidin and avidin homologues as larvicides against insect pests.
  • An enzyme inhibitor e.g., a protease or proteinase inhibitor or an amylase inhibitor.
  • a protease or proteinase inhibitor or an amylase inhibitor See, e.g., Abe et ai, J, Biol. Chem. 262: 16793 (1987) (nucleotide sequence of rice cysteine proteinase inhibitor); Huub et ai, Plant Molec. Biol. 21 :985 (1993) (nucleotide sequence of cDNA encoding tobacco proteinase inhibitor 1); and Sura ⁇ ani et ai, Biosci. Biotech. Biocheni. 57: 1243 (1993) (nucleotide sequence of Streptomyces nitrosporeus-amylase inhibitor).
  • F An insect-specific hormone or pheromone such as an ecdysteroid and juvenile hormone, a variant thereof, a mimetic based thereon, or an antagonist or agonist thereof.
  • an insect-specific hormone or pheromone such as an ecdysteroid and juvenile hormone, a variant thereof, a mimetic based thereon, or an antagonist or agonist thereof.
  • Sec e.g., Hammock et ah, Nature, 344:458 (1990), disclosing baculovirus expression of cloned juvenile hormone esterase, an inactivator of juvenile hormone.
  • G An insect-specific peptide or neuropeptide that, upon expression, disrupts the physiology of the affected pest. See, e.g., Regan. J. Biol. Chem. 269:9 (1994) (expression cloning yields DNA coding for insect diuretic hormone receptor); and Pratt et al, Biocheni. Biophys. Res. Comm., 163: 1243 ( 1989) (an allostatin in Diploptera puntata). See also U.S. Pat. No. 5,266,317 to Toraalski et ai, disclosing genes encoding insect-specific, paralytic neurotoxins.
  • H An insect-specific venom produced in nature by a snake, a wasp, etc. For example, see Pang et ai, Gene, 1 16: 165 (1992), concerning heterologous expression in plants of a gene coding for a scorpion insectotoxic peptide.
  • I An enzyme responsible for hyperaccumulation of a monolerpene, a s ⁇ squil ⁇ ene, a steroid, hydroxamic acid, a phenylpropanoid derivative or another non-protein molecule with insccticidaj activity.
  • An enzyme involved in the modification, including post-translational modification, of a biologically active molecule e.g., a glycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme, a nuclease, a cyclase, a transaminase, an esterase, a hydrolase, a phosphatase, a kinase, a phosphoryiase, a polymerase, an elastase, a chitinase, or a glucanase, either natural or synthetic.
  • a glycolytic enzyme e.g., a proteolytic enzyme, a lipolytic enzyme, a nuclease, a cyclase, a transaminase, an esterase, a hydrolase, a phosphatase, a kinase, a phosphoryiase, a polymerase, an elastase, a chit
  • DNA molecules that contain chitinase- encoding sequences can be obtained, for example, from the American Type Culture Collection under Accession Nos. 39637 and 67152. See also Kramer et ai, Insect Biochem. Molec, Biol. 23:691 (1993), which discloses the nucleotide sequence of a cDlMA encoding tobacco hookworm chitinase; and Kawalleck et ai, Plant Molec. Biol, 21 :673 ( 1993), which discloses the nucleotide sequence of the parsley ubi4-2 polyubiquilin gene.
  • K A molecule that stimulates signal transduction. See, e.g., BoteJla et ai, Plant Molec. Biol., 24:757 (1994), which discloses nucleotide sequences for mung bean calmodulin cDNA clones; and Griess et ai.. Plant Physiol., 104: 1467 ( 1994), which discloses the nucleotide sequence of a maize calmodulin cDNA clone.
  • M A membrane permease, a channel former or a channel blocker. See, e.g., Jayn ⁇ s el ai, Plant ScL, 89:43 (1993), which discloses heterologous expression of a cccropin lytic peptide analog to render transgenic tobacco plants resistant to Pseudoinonas solanacearum.
  • N A viral-invasive protein or a complex toxin derived therefrom.
  • the accumulation of viral coat proteins in transformed plant cells induces resistance to viral infection or disease development caused by the virus from which the coat protein gene is derived, as well as by related viruses.
  • Coat protein-mediated resistance has been conferred upon transformed plants against alfalfa mosaic virus, cucumber mosaic virus, tobacco streak virus, potato virus X, potato virus Y, tobacco etch virus, tobacco rattle virus, and tobacco mosaic virus. See Beachy et al, Ann. Rev. PhytopathoL, 28:451 (1990).
  • P. A virus-specific antibody See, e.g., Tavladoraki et a!,, Nature, 366:469 (1993), showing protection of transgenic plants expressing recombinant antibody genes from virus attack.
  • a developmental-arrest protein produced in nature by a pathogen or a parasite For example, fungal em/ ⁇ f>l ,4-D- ⁇ o3ygalaeturonases facilitate fungal colonization and plant nutrient release by solubilizing plant cell wall bomo- 1 ,4-D-gaJacturonasc. Sec Lamb et al, Bio/Technology, 10: 1436 (1992). The cloning and characterization of a gene that encodes a bean endopolygalacturonase-inhibiting protein is described by Toubart et al, Plant J., 2:367 (1992).
  • a herbicide that inhibits the growing point or meristem such as an imidazolinone or a sulfonylurea.
  • Exemplar ⁇ ' genes in this category code for mutant ALS and AFiAS enzymes as described, for example, by Lee et al, EMBO J., 7: 1241 (1988); and Miki et al, Theor. Appl. Genet, 80:449 (1990), respectively. See, additionally, U.S.
  • Resistance to AHAS-acting herbicides may be through a mechanism other than a resistant AHAS enzyme. See, e.g., U.S. Patent No. 5,545,822.
  • nucleotide sequence of a phosphinothricin-aectyl- transferas ⁇ gene is provided in European Application No. 0242246 to Leemans et al. and DeGreef et al * 7:61 (1989), describing the production of transgenic plants that express chimeric bar genes coding for phosphinothricin acetyl transferase activity.
  • genes conferring resistance to phenoxy propionic acids and cyclohexones, such as sethoxydim and haloxyfop are the Accl-S l , Aeel -S2, and Ace 1 -S3 genes described by Marshall et al, Theor. Appl Gem!.. 83:435 (1992).
  • a herbicide that inhibits photosynthesis such as a triazine (psbA and gs+ genes) or a benzonilrile (nitrilase gene).
  • Przibilla et al. Plant Cell, 3: 169 ( 1991 ) describe the transformation of Chlamydomonas with plasmids encoding mutant psbA genes. Nucleotide sequences for nitrilase genes arc disclosed in U.S. Pat. No. 4,810,648 to Stalker, and DNA molecules containing these genes are available under ATCC Accession Nos. 53435, 67441, and 67442.
  • a gene may be introduced to reduce phytate content. For example, this may be accomplished by cloning, and then reintroducing DNA associated with an allele that is responsible for maize mutants characterized by low levels of phytic acid, or a homologous or analogous mutation in rice may be used. See Raboy et aL, Maydlca, 35:383 (1990).
  • Carbohydrate composition may be modified, for example, by transforming plants with a gene coding for an enzyme that alters the branching pattern of starch. See Shiroza et aL, J. BacteoL, 170:810 (1988) (nucleotide sequence of Streptococcus mutant fructosyltransferase gene); Steinmetz el aL, MoL Gen.
  • a method for introducing an expression vector into plants is based on the natural transformation system of Agrobacterium. See, e.g., Horsch el al.. Science, 227: 1229 (1985).
  • A, nimefaciens and A. rhizogenes are plant pathogenic soil bacteria that genetically transform plant cells.
  • a generally applicable method of plant transformation is microprojecfile- racdiated (so-called "gene gun") transformation, in which DNA is carried on the surface of microprojectiles, typically 1 to 4 ⁇ m in diameter.
  • the expression vector is introduced into plant tissues with a biolistic device that accelerates the microprojectiles to typical speeds of 300 to 600 m/ ' s, sufficient to penetrate plant cell walls and membranes.
  • a biolistic device that accelerates the microprojectiles to typical speeds of 300 to 600 m/ ' s, sufficient to penetrate plant cell walls and membranes.
  • Various target tissues may be bombarded with DNA- coated rnicroprojectiles to produce transgenic plants, including, for example, callus (Type 1 or Type II), immature embryos, and meristematic tissue.
  • transgenic inbred line may then be crossed with another inbred line (itself either transformed or non-transformed), to produce a new transgenic inbred line.
  • a genetic trait that has been engineered into a particular rice line may be moved into another line using traditional crossing and backcrossing techniques. For example, backcrossing may be used to move an engineered trait from a public, non-elite inbred line into an elite inbred line, or from an inbred line containing a foreign gene in its genome into an inbred line or lines that do not contain that gene.
  • inbred rice plant should be understood also to include single gene conversions of an inbred line.
  • Backcrossing methods can be used with the present invention to improve or introduce a characteristic into an inbred line.
  • Single gene traits have been identified that are not regularly selected for in the development of a new inbred line, but that may be improved by crossing and backcrossing.
  • Single gene traits may or may not be transgenic. Examples of such traits include male sterility, waxy starch, herbicide resistance, resistance for bacterial or fungal or viral disease, insect resistance, male fertility, enhanced nutritional quality, yield stability, and yield enhancement. These genes are generally inherited through the nucleus. Known exceptions to the nuclear genes include some genes for male sterility that are inherited cytoplasmically, but that still act functionally as single gene traits.
  • Several single gene traits arc described in U.S. Patent Mos. 5,777,196; 5,948,957; and 5,969,212.

Abstract

La présente invention concerne des hybrides et des cultivars dérivés du cultivar de riz appelé « CL151 ». L’invention concerne des semences et des plantes de riz hybride produites par croisement du cultivar « CL151 » avec un autre cultivar de riz. L’invention concerne en outre d’autres dérivés du cultivar de riz « CL151 ».
PCT/US2009/064883 2008-11-20 2009-11-18 Hybrides de, et cultivars dérivés du cultivar de riz appelé « cl151 » WO2010059656A1 (fr)

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MX2011005356A MX345887B (es) 2008-11-20 2009-11-18 Hibridos de, y variedades derivadas de la variedad de arroz designada cl151.
EP09828127A EP2358193A4 (fr) 2008-11-20 2009-11-18 Hybrides de, et cultivars dérivés du cultivar de riz appelé 'cl151'
US13/129,860 US8946528B2 (en) 2008-11-20 2009-11-18 Hybrids of, and cultivars derived from the rice cultivar designated ‘CL151’
US14/558,763 US9499834B2 (en) 2008-11-20 2014-12-03 Rice cultivar designated ‘CL151’

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US14/558,763 Continuation-In-Part US9499834B2 (en) 2008-11-20 2014-12-03 Rice cultivar designated ‘CL151’
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EP2485581A1 (fr) * 2009-10-08 2012-08-15 Board of Supervisors of Lousiana State University Cultivar de riz baptisé 'cl261'
CN102948363A (zh) * 2012-10-26 2013-03-06 袁隆平农业高科技股份有限公司 一种杂交水稻的制种方法
EP2690946A1 (fr) * 2011-03-31 2014-02-05 Board of Supervisors of Lousiana State University Cultivar de riz appelé « cl152 »
US10064355B2 (en) 2013-12-09 2018-09-04 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Rice cultivar designated ‘CL271’

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WO2011044006A1 (fr) 2009-10-08 2011-04-14 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Cultivar de riz dénommé « cl111 »
US9888637B2 (en) 2015-11-17 2018-02-13 Louisiana State University Agricultural Center LSUAC Hi Protein Rice
CN108432671B (zh) * 2018-03-27 2021-06-01 南京师范大学 一种利用回交育种创制黄颡鱼新品系的方法
US11612125B2 (en) 2018-04-25 2023-03-28 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Rice cultivar designated ‘CLJ01’
CN111869525B (zh) * 2020-07-22 2022-03-25 周纯有 一种水育稻苗的育苗方法

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EP2485581A1 (fr) * 2009-10-08 2012-08-15 Board of Supervisors of Lousiana State University Cultivar de riz baptisé 'cl261'
EP2485581A4 (fr) * 2009-10-08 2013-10-02 Supervisors Of Lousiana State University Board Of Cultivar de riz baptisé 'cl261'
EP2690946A1 (fr) * 2011-03-31 2014-02-05 Board of Supervisors of Lousiana State University Cultivar de riz appelé « cl152 »
EP2690946A4 (fr) * 2011-03-31 2014-11-05 Supervisors Of Lousiana State University Board Of Cultivar de riz appelé « cl152 »
CN102948363A (zh) * 2012-10-26 2013-03-06 袁隆平农业高科技股份有限公司 一种杂交水稻的制种方法
CN102948363B (zh) * 2012-10-26 2014-05-14 袁隆平农业高科技股份有限公司 一种杂交水稻的制种方法
US10064355B2 (en) 2013-12-09 2018-09-04 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Rice cultivar designated ‘CL271’

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UY32254A (es) 2010-06-30
EP2358193A1 (fr) 2011-08-24
EP2358193A4 (fr) 2012-05-09
US8946528B2 (en) 2015-02-03
MX2011005356A (es) 2012-03-26
US9499834B2 (en) 2016-11-22
CR20110333A (es) 2012-01-07
US20150096074A1 (en) 2015-04-02
US20110296548A1 (en) 2011-12-01
AR074385A1 (es) 2011-01-12

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