WO2010041863A8 - 지방세포 분화용 배지 조성물 - Google Patents

지방세포 분화용 배지 조성물 Download PDF

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Publication number
WO2010041863A8
WO2010041863A8 PCT/KR2009/005714 KR2009005714W WO2010041863A8 WO 2010041863 A8 WO2010041863 A8 WO 2010041863A8 KR 2009005714 W KR2009005714 W KR 2009005714W WO 2010041863 A8 WO2010041863 A8 WO 2010041863A8
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WO
WIPO (PCT)
Prior art keywords
adipocyte differentiation
medium composition
differentiation medium
dhea
media composition
Prior art date
Application number
PCT/KR2009/005714
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English (en)
French (fr)
Other versions
WO2010041863A2 (ko
WO2010041863A3 (ko
WO2010041863A9 (ko
Inventor
조대호
방사익
송석빈
천소영
정민경
박유림
Original Assignee
숙명여자대학교산학협력단
사회복지법인 삼성생명공익재단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 숙명여자대학교산학협력단, 사회복지법인 삼성생명공익재단 filed Critical 숙명여자대학교산학협력단
Publication of WO2010041863A2 publication Critical patent/WO2010041863A2/ko
Publication of WO2010041863A9 publication Critical patent/WO2010041863A9/ko
Publication of WO2010041863A8 publication Critical patent/WO2010041863A8/ko
Publication of WO2010041863A3 publication Critical patent/WO2010041863A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/82—Histamine

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

본 발명은 지방세포 분화용 배지 조성물에 있어서, 인슐린(insulin), DHEA(dehydroepiandrosterone) 및 히스타민(histamine)을 함유하는 것을 특징으로 하는 지방세포 분화용 배지 조성물 및 상기 배지 조성물을 이용하여 지방전구세포(preadipocyte)를 지방세포(adipocyte)로 분화시키는 방법에 관한 것이다. 본 발명의 지방세포 분화용 배지 조성물은 통상적으로 사용되는 지방세포 분화용 배지 조성물에 비해 분화된 지방세포의 분포가 고르고 분화된 지방세포가 안정적으로 고정될 수 있도록 하여 생존율을 극대화할 수 있는 장점이 있다. 또한 본 발명의 지방세포 분화용 배지 조성물에 사용되는 DHEA(dehydroepiandrosterone) 및 히스타민(histamine)은 종래 사용되던 덱사메타손(dexamethasone) 및 아이소부틸메틸젠틴(isobutylmethylxanthine)에 비하여 가격이 저렴하므로 경제적인 장점이 있다.
PCT/KR2009/005714 2008-10-07 2009-10-07 지방세포 분화용 배지 조성물 WO2010041863A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020080098149A KR101019877B1 (ko) 2008-10-07 2008-10-07 지방세포 분화용 배지 조성물
KR10-2008-0098149 2008-10-07

Publications (4)

Publication Number Publication Date
WO2010041863A2 WO2010041863A2 (ko) 2010-04-15
WO2010041863A9 WO2010041863A9 (ko) 2010-06-10
WO2010041863A8 true WO2010041863A8 (ko) 2010-07-29
WO2010041863A3 WO2010041863A3 (ko) 2010-09-16

Family

ID=42101078

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/005714 WO2010041863A2 (ko) 2008-10-07 2009-10-07 지방세포 분화용 배지 조성물

Country Status (2)

Country Link
KR (1) KR101019877B1 (ko)
WO (1) WO2010041863A2 (ko)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100865884B1 (ko) * 2003-03-18 2008-10-31 (주)안트로젠 동종 이식에 사용하기 위한 분화된 지방 세포
US7893315B2 (en) * 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
CN101501185A (zh) 2006-06-09 2009-08-05 人类起源公司 胎盘巢(placental niche)及其培养干细胞的用途
KR100883565B1 (ko) * 2007-02-14 2009-02-13 (주)프로스테믹스 지방 줄기세포의 조직 재생 능력을 최적화한 조건 배지를 함유한 조직 재생용 주사제 첨가제

Also Published As

Publication number Publication date
WO2010041863A2 (ko) 2010-04-15
WO2010041863A3 (ko) 2010-09-16
KR101019877B1 (ko) 2011-03-04
WO2010041863A9 (ko) 2010-06-10
KR20100038967A (ko) 2010-04-15

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