WO2010041863A3 - 지방세포 분화용 배지 조성물 - Google Patents

지방세포 분화용 배지 조성물 Download PDF

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Publication number
WO2010041863A3
WO2010041863A3 PCT/KR2009/005714 KR2009005714W WO2010041863A3 WO 2010041863 A3 WO2010041863 A3 WO 2010041863A3 KR 2009005714 W KR2009005714 W KR 2009005714W WO 2010041863 A3 WO2010041863 A3 WO 2010041863A3
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WIPO (PCT)
Prior art keywords
adipocyte differentiation
medium composition
differentiation medium
dhea
media composition
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PCT/KR2009/005714
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English (en)
French (fr)
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WO2010041863A2 (ko
WO2010041863A9 (ko
WO2010041863A8 (ko
Inventor
조대호
방사익
송석빈
천소영
정민경
박유림
Original Assignee
숙명여자대학교산학협력단
사회복지법인 삼성생명공익재단
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Application filed by 숙명여자대학교산학협력단, 사회복지법인 삼성생명공익재단 filed Critical 숙명여자대학교산학협력단
Publication of WO2010041863A2 publication Critical patent/WO2010041863A2/ko
Publication of WO2010041863A9 publication Critical patent/WO2010041863A9/ko
Publication of WO2010041863A8 publication Critical patent/WO2010041863A8/ko
Publication of WO2010041863A3 publication Critical patent/WO2010041863A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0653—Adipocytes; Adipose tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/82—Histamine

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

본 발명은 지방세포 분화용 배지 조성물에 있어서, 인슐린(insulin), DHEA(dehydroepiandrosterone) 및 히스타민(histamine)을 함유하는 것을 특징으로 하는 지방세포 분화용 배지 조성물 및 상기 배지 조성물을 이용하여 지방전구세포(preadipocyte)를 지방세포(adipocyte)로 분화시키는 방법에 관한 것이다. 본 발명의 지방세포 분화용 배지 조성물은 통상적으로 사용되는 지방세포 분화용 배지 조성물에 비해 분화된 지방세포의 분포가 고르고 분화된 지방세포가 안정적으로 고정될 수 있도록 하여 생존율을 극대화할 수 있는 장점이 있다. 또한 본 발명의 지방세포 분화용 배지 조성물에 사용되는 DHEA(dehydroepiandrosterone) 및 히스타민(histamine)은 종래 사용되던 덱사메타손(dexamethasone) 및 아이소부틸메틸젠틴(isobutylmethylxanthine)에 비하여 가격이 저렴하므로 경제적인 장점이 있다.
PCT/KR2009/005714 2008-10-07 2009-10-07 지방세포 분화용 배지 조성물 WO2010041863A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020080098149A KR101019877B1 (ko) 2008-10-07 2008-10-07 지방세포 분화용 배지 조성물
KR10-2008-0098149 2008-10-07

Publications (4)

Publication Number Publication Date
WO2010041863A2 WO2010041863A2 (ko) 2010-04-15
WO2010041863A9 WO2010041863A9 (ko) 2010-06-10
WO2010041863A8 WO2010041863A8 (ko) 2010-07-29
WO2010041863A3 true WO2010041863A3 (ko) 2010-09-16

Family

ID=42101078

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/005714 WO2010041863A2 (ko) 2008-10-07 2009-10-07 지방세포 분화용 배지 조성물

Country Status (2)

Country Link
KR (1) KR101019877B1 (ko)
WO (1) WO2010041863A2 (ko)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040082068A (ko) * 2003-03-18 2004-09-24 (주)안트로젠 동종 이식에 사용하기 위한 분화된 지방 세포
WO2007146123A2 (en) * 2006-06-09 2007-12-21 Anthrogenesis Corporation Placental niche and use thereof to culture stem cells
US20080057041A1 (en) * 2004-11-04 2008-03-06 Chung Young G Derivation of embryonic stem cells and embryo-derived cells
KR20080075959A (ko) * 2007-02-14 2008-08-20 (주)프로스테믹스 지방 줄기세포의 조직 재생 능력을 최적화한 배양액을 함유한 조직 재생용 주사제 조성물

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040082068A (ko) * 2003-03-18 2004-09-24 (주)안트로젠 동종 이식에 사용하기 위한 분화된 지방 세포
US20080057041A1 (en) * 2004-11-04 2008-03-06 Chung Young G Derivation of embryonic stem cells and embryo-derived cells
WO2007146123A2 (en) * 2006-06-09 2007-12-21 Anthrogenesis Corporation Placental niche and use thereof to culture stem cells
KR20080075959A (ko) * 2007-02-14 2008-08-20 (주)프로스테믹스 지방 줄기세포의 조직 재생 능력을 최적화한 배양액을 함유한 조직 재생용 주사제 조성물

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DWIGHT J. KLEMM ET AL.: "Insulin-induced Adipocyte Differentiation.", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 30, 27 July 2001 (2001-07-27), pages 28430 - 28435 *

Also Published As

Publication number Publication date
KR20100038967A (ko) 2010-04-15
KR101019877B1 (ko) 2011-03-04
WO2010041863A2 (ko) 2010-04-15
WO2010041863A9 (ko) 2010-06-10
WO2010041863A8 (ko) 2010-07-29

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