WO2010040496A3 - Location-specific recombination for activating genes in genetic systems - Google Patents

Location-specific recombination for activating genes in genetic systems Download PDF

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Publication number
WO2010040496A3
WO2010040496A3 PCT/EP2009/007148 EP2009007148W WO2010040496A3 WO 2010040496 A3 WO2010040496 A3 WO 2010040496A3 EP 2009007148 W EP2009007148 W EP 2009007148W WO 2010040496 A3 WO2010040496 A3 WO 2010040496A3
Authority
WO
WIPO (PCT)
Prior art keywords
location
specific recombination
genetic systems
activating genes
target gene
Prior art date
Application number
PCT/EP2009/007148
Other languages
German (de)
French (fr)
Other versions
WO2010040496A2 (en
Inventor
Ralph Bertram
Original Assignee
Universität Tübingen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universität Tübingen filed Critical Universität Tübingen
Publication of WO2010040496A2 publication Critical patent/WO2010040496A2/en
Publication of WO2010040496A3 publication Critical patent/WO2010040496A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to the use of identification sequences for location-specific recombinases, the identification sequences each containing two recombinase binding sequences that are separated by a spacer sequence, in order to regulate, especially activate, a target gene in a genetic system. The invention also relates to the corresponding use of a location-specific recombination system as well as a method for regulating, especially activating, a target gene in a genetic system.
PCT/EP2009/007148 2008-10-06 2009-10-06 Location-specific recombination for activating genes in genetic systems WO2010040496A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008051708.9 2008-10-06
DE102008051708A DE102008051708A1 (en) 2008-10-06 2008-10-06 Site-specific recombination for gene activation in genetic systems

Publications (2)

Publication Number Publication Date
WO2010040496A2 WO2010040496A2 (en) 2010-04-15
WO2010040496A3 true WO2010040496A3 (en) 2010-11-04

Family

ID=41795163

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/007148 WO2010040496A2 (en) 2008-10-06 2009-10-06 Location-specific recombination for activating genes in genetic systems

Country Status (2)

Country Link
DE (1) DE102008051708A1 (en)
WO (1) WO2010040496A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000058488A2 (en) * 1999-03-31 2000-10-05 Invitrogen Corporation Delivery of functional protein sequences by translocating polypeptides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10140030C1 (en) * 2001-08-16 2002-12-19 Gsf Forschungszentrum Umwelt Method for performing multiple recombination events in a genetic system, useful e.g. for removing antibiotic resistance genes, uses mutant recombinase recognition sites

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000058488A2 (en) * 1999-03-31 2000-10-05 Invitrogen Corporation Delivery of functional protein sequences by translocating polypeptides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHENG TZU-HAO ET AL: "Controlling gene expression in yeast by inducible site-specific recombination", NUCLEIC ACIDS RESEARCH, vol. 28, no. 24, December 2000 (2000-12-01), XP002580124, ISSN: 0305-1048 *
HUANG Z JOSH ET AL: "Cre/loxP recombination-activated neuronal markers in mouse neocortex and hippocampus", GENESIS THE JOURNAL OF GENETICS AND DEVELOPMENT, vol. 32, no. 3, March 2002 (2002-03-01), pages 209 - 217, XP002580123, ISSN: 1526-954X *
SPIOTTO M T ET AL: "Floxed reporter genes: Flow-cytometric selection of clonable cells expressing high levels of a target gene after tamoxifen-regulated Cre-loxP recombination", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL LNKD- DOI:10.1016/J.JIM.2006.02.016, vol. 312, no. 1-2, 30 May 2006 (2006-05-30), pages 201 - 208, XP025158186, ISSN: 0022-1759, [retrieved on 20060530] *
ZHU HUAN-ZHANG ET AL: "Conditional gene activation in cultured hepatocytes using a ligand-dependent chimeric Cre recombinase.", SHENGWU HUAXUE YU SHENGWU WULI XUEBAO, vol. 35, no. 5, May 2003 (2003-05-01), pages 435 - 440, XP002580122, ISSN: 0582-9879 *

Also Published As

Publication number Publication date
DE102008051708A1 (en) 2010-04-08
WO2010040496A2 (en) 2010-04-15

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