WO2010021001A3 - Process for preparing sweetener from stevia rebaudiana - Google Patents

Process for preparing sweetener from stevia rebaudiana Download PDF

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Publication number
WO2010021001A3
WO2010021001A3 PCT/IN2009/000456 IN2009000456W WO2010021001A3 WO 2010021001 A3 WO2010021001 A3 WO 2010021001A3 IN 2009000456 W IN2009000456 W IN 2009000456W WO 2010021001 A3 WO2010021001 A3 WO 2010021001A3
Authority
WO
WIPO (PCT)
Prior art keywords
color
bacteria
polyphenols
reduced
stevia extracts
Prior art date
Application number
PCT/IN2009/000456
Other languages
French (fr)
Other versions
WO2010021001A2 (en
Inventor
Ramakrishnan S. Kaushik
Antony Usha
Muthukumaran Geetha
Original Assignee
Kaushik Ramakrishnan S
Antony Usha
Muthukumaran Geetha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaushik Ramakrishnan S, Antony Usha, Muthukumaran Geetha filed Critical Kaushik Ramakrishnan S
Publication of WO2010021001A2 publication Critical patent/WO2010021001A2/en
Publication of WO2010021001A3 publication Critical patent/WO2010021001A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides

Abstract

The present invention provides a biological method for removing the color and odor causing components of stevia extracts and for producing a sweetener product which retains the beneficial anti-oxidant activity of a crude extract. Bacteria and fungi are capable of degrading plant pigments and polyphenols responsible for causing color in stevia extracts. Pleurotus ostreatus was employed as a test organism and reduced color by 70% and 50% in the yellow-brown (470 nm) and green (670 nm) region. Total polyphenol content was reduced by 40% but the free radical scavenging activity of the extract was maintained. Any bacteria or fungus capable of degrading plant pigments and polyphenols, but do not degrade stevioside, may be utilized in this method.
PCT/IN2009/000456 2008-08-19 2009-08-17 Process for preparing sweetener from stevia rebaudiana WO2010021001A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2010/CHE/2008 2008-08-19
IN2010CH2008 2008-08-19

Publications (2)

Publication Number Publication Date
WO2010021001A2 WO2010021001A2 (en) 2010-02-25
WO2010021001A3 true WO2010021001A3 (en) 2010-04-29

Family

ID=41667213

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IN2009/000456 WO2010021001A2 (en) 2008-08-19 2009-08-17 Process for preparing sweetener from stevia rebaudiana

Country Status (1)

Country Link
WO (1) WO2010021001A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY180803A (en) 2010-06-02 2020-12-09 Evolva Inc Recombinant production of steviol glycosides
SG10201606565XA (en) 2011-08-08 2016-10-28 Evolva Sa Recombinant production of steviol glycosides
JP6379112B2 (en) 2013-02-06 2018-08-22 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． Improved production method of rebaudioside D and rebaudioside M
US10017804B2 (en) 2013-02-11 2018-07-10 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
US10421983B2 (en) 2014-08-11 2019-09-24 Evolva Sa Production of steviol glycosides in recombinant hosts
CN107109358B (en) 2014-09-09 2022-08-02 埃沃尔瓦公司 Production of steviol glycosides in recombinant hosts
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
US10604743B2 (en) 2015-03-16 2020-03-31 Dsm Ip Assets B.V. UDP-glycosyltransferases
EP3332018B1 (en) 2015-08-07 2022-07-27 Evolva SA Production of steviol glycosides in recombinant hosts
MX2018012561A (en) 2016-04-13 2019-01-17 Evolva Sa Production of steviol glycosides in recombinant hosts.
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
GB202002078D0 (en) * 2020-02-14 2020-04-01 Lucozade Ribena Suntory Ltd Sweetening ingredients

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5917996A (en) * 1982-07-21 1984-01-30 Dainippon Ink & Chem Inc Preparation of rebaudioside a
JPS59102372A (en) * 1982-12-02 1984-06-13 Kiyo Kondo Method for improving taste of stevioside
US4590160A (en) * 1982-02-27 1986-05-20 Dainippon Ink And Chemicals, Inc. Process for production of β-glycosyl stevioside derivatives
US4892938A (en) * 1987-07-21 1990-01-09 Giovanetto Roger H Method for the recovery of steviosides from plant raw material
US5112610A (en) * 1988-03-29 1992-05-12 Udo Kienle Method of making a natural sweetener based on Stevia rebaudiana, and use thereof
EP0525740A1 (en) * 1991-08-01 1993-02-03 Dozono, Fumio Stevia extract-containing liquid internal medicine and method of preparing the same
JPH06298791A (en) * 1993-04-20 1994-10-25 Nippon Paper Ind Co Ltd Stevioside-sugar transfer compound and its production
WO2003035090A1 (en) * 2001-10-25 2003-05-01 Kabushiki Kaisha Thieisutebia Agents relieving anaphylactic allergic reactions and process for producing the same
WO2005112668A1 (en) * 2004-05-24 2005-12-01 Soo Sang Park Manufacturing method of the stevia-extracts
US20060134292A1 (en) * 2004-12-21 2006-06-22 Stevian Biotechnology Corporation Sdn. Bhd. Malaysia Extraction, separation and modification of sweet glycosides from the stevia rebaudiana plant
KR20070033056A (en) * 2005-04-16 2007-03-26 한국스테비아(주) Manufacturing method of stevia extract

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4590160A (en) * 1982-02-27 1986-05-20 Dainippon Ink And Chemicals, Inc. Process for production of β-glycosyl stevioside derivatives
JPS5917996A (en) * 1982-07-21 1984-01-30 Dainippon Ink & Chem Inc Preparation of rebaudioside a
JPS59102372A (en) * 1982-12-02 1984-06-13 Kiyo Kondo Method for improving taste of stevioside
US4892938A (en) * 1987-07-21 1990-01-09 Giovanetto Roger H Method for the recovery of steviosides from plant raw material
US5112610A (en) * 1988-03-29 1992-05-12 Udo Kienle Method of making a natural sweetener based on Stevia rebaudiana, and use thereof
EP0525740A1 (en) * 1991-08-01 1993-02-03 Dozono, Fumio Stevia extract-containing liquid internal medicine and method of preparing the same
JPH06298791A (en) * 1993-04-20 1994-10-25 Nippon Paper Ind Co Ltd Stevioside-sugar transfer compound and its production
WO2003035090A1 (en) * 2001-10-25 2003-05-01 Kabushiki Kaisha Thieisutebia Agents relieving anaphylactic allergic reactions and process for producing the same
WO2005112668A1 (en) * 2004-05-24 2005-12-01 Soo Sang Park Manufacturing method of the stevia-extracts
US20060134292A1 (en) * 2004-12-21 2006-06-22 Stevian Biotechnology Corporation Sdn. Bhd. Malaysia Extraction, separation and modification of sweet glycosides from the stevia rebaudiana plant
KR20070033056A (en) * 2005-04-16 2007-03-26 한국스테비아(주) Manufacturing method of stevia extract

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 198410, Derwent World Patents Index; AN 1984-059776, XP002569571 *
DATABASE WPI Week 198430, Derwent World Patents Index; AN 1984-184627, XP002569572 *
DATABASE WPI Week 199502, Derwent World Patents Index; AN 1995-011832, XP002569573 *
DATABASE WPI Week 200338, Derwent World Patents Index; AN 2003-403301, XP002569574 *
DATABASE WPI Week 200770, Derwent World Patents Index; AN 2007-748642, XP002569575 *

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Publication number Publication date
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