WO2010003614A3 - Transfektionslösung - Google Patents

Transfektionslösung Download PDF

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Publication number
WO2010003614A3
WO2010003614A3 PCT/EP2009/004870 EP2009004870W WO2010003614A3 WO 2010003614 A3 WO2010003614 A3 WO 2010003614A3 EP 2009004870 W EP2009004870 W EP 2009004870W WO 2010003614 A3 WO2010003614 A3 WO 2010003614A3
Authority
WO
WIPO (PCT)
Prior art keywords
transfection
transfection solution
solution
producing
complexes
Prior art date
Application number
PCT/EP2009/004870
Other languages
English (en)
French (fr)
Other versions
WO2010003614A2 (de
Inventor
Jörg DENNING
Kerstin Goebels
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Publication of WO2010003614A2 publication Critical patent/WO2010003614A2/de
Publication of WO2010003614A3 publication Critical patent/WO2010003614A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/06—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom containing only hydrogen and carbon atoms in addition to the ring nitrogen atom
    • C07D213/16—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom containing only hydrogen and carbon atoms in addition to the ring nitrogen atom containing only one pyridine ring
    • C07D213/20—Quaternary compounds thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft eine Transfektionslösung zur Herstellung von Transfektionskomplexen, enthaltend wenigstens einen Puffer, vorzugsweise einen HEPES-Puffer, und wenigstens ein Transfektionsreagenz, das ein kationisches Lipid aufweist. Erfindungsgemäß wird kein Kulturmedium zur Herstellung der Transfektionslösung eingesetzt. Dies verbessert die Langzeitstabilität der Transfektionslösung, so dass sie über einen längeren Zeitraum zur Herstellung von Transfektionskomplexen mit der zu transfizierenden Substanz eingesetzt werden kann.
PCT/EP2009/004870 2008-07-11 2009-07-06 Transfektionslösung WO2010003614A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008032594.5 2008-07-11
DE200810032594 DE102008032594A1 (de) 2008-07-11 2008-07-11 Transfektionslösung

Publications (2)

Publication Number Publication Date
WO2010003614A2 WO2010003614A2 (de) 2010-01-14
WO2010003614A3 true WO2010003614A3 (de) 2010-08-26

Family

ID=41258619

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/004870 WO2010003614A2 (de) 2008-07-11 2009-07-06 Transfektionslösung

Country Status (2)

Country Link
DE (1) DE102008032594A1 (de)
WO (1) WO2010003614A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ2020529A3 (cs) * 2020-09-23 2022-03-30 Ăšstav organickĂ© chemie a biochemie AV ÄŚR, v. v. i. Lipidoidy pro transfekci nukleových kyselin a jejich použití

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0755924A1 (de) * 1995-07-25 1997-01-29 Stichting voor de Technische Wetenschappen Transportvehikels für Makromoleküle

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19605175A1 (de) 1996-02-13 1997-08-14 Sourovoi Andrej Dr Lipidverbindungen und deren Verwendung
EP1254896A1 (de) 2001-05-03 2002-11-06 Applied NanoSystems B.V. Kationische Amphiphile, Verfahren und Methoden zur Transfektion
NL1027479C2 (nl) 2004-10-21 2006-05-01 Synvolux Ip B V Bescherming van biologisch actieve moleculen met behulp van amphifielen.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0755924A1 (de) * 1995-07-25 1997-01-29 Stichting voor de Technische Wetenschappen Transportvehikels für Makromoleküle

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ILIES M A ET AL: "Pyridinium cationic lipids in gene delivery: an in vitro and in vivo comparison of transfection efficiency versus a tetraalkylammonium congener", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, ACADEMIC PRESS, US, vol. 435, no. 1, 1 March 2005 (2005-03-01), pages 217 - 226, XP004725960, ISSN: 0003-9861 *
LOBO BRIAN A ET AL: "Isothermal titration calorimetric analysis of the interaction between cationic lipids and plasmid DNA", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 386, no. 1, 1 February 2001 (2001-02-01), pages 95 - 105, XP002554643, ISSN: 0003-9861 *
MADEIRA CATARINA ET AL: "Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET", BMC BIOTECHNOLOGY, BIOMED CENTRAL LTD. LONDON, GB, vol. 8, no. 1, 26 February 2008 (2008-02-26), pages 20, XP021035690, ISSN: 1472-6750 *

Also Published As

Publication number Publication date
WO2010003614A2 (de) 2010-01-14
DE102008032594A1 (de) 2010-01-14

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