WO2009152209A3 - Test de détection d'analyte visuel/par fluorescence combiné - Google Patents

Test de détection d'analyte visuel/par fluorescence combiné Download PDF

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Publication number
WO2009152209A3
WO2009152209A3 PCT/US2009/046848 US2009046848W WO2009152209A3 WO 2009152209 A3 WO2009152209 A3 WO 2009152209A3 US 2009046848 W US2009046848 W US 2009046848W WO 2009152209 A3 WO2009152209 A3 WO 2009152209A3
Authority
WO
WIPO (PCT)
Prior art keywords
test line
visible
fluorescing
spectrum
analyte detection
Prior art date
Application number
PCT/US2009/046848
Other languages
English (en)
Other versions
WO2009152209A2 (fr
Inventor
Uma Mahesh Babu
Robert W. Vandine
Robert P. Sambursky
Ganga Kanaujia
Richard Rivas, Jr.
Original Assignee
Rapid Pathogen Screening, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rapid Pathogen Screening, Inc. filed Critical Rapid Pathogen Screening, Inc.
Publication of WO2009152209A2 publication Critical patent/WO2009152209A2/fr
Publication of WO2009152209A3 publication Critical patent/WO2009152209A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Selon l'invention, la sensibilité de tests d'immunodosage à écoulement latéral et à lecture visuelle est améliorée par l'addition d'une petite quantité de colorant fluorescent ou de conjugués de particules de latex fluorescentes au matériau conjugué initial. Lorsque la ligne de test de spectre visible est visiblement présente, le résultat du test est observé et enregistré. Cependant, dans le cas où le résultat est indéterminé, une lumière d'un spectre approprié, telle qu'un spectre ultraviolet, visible ou infrarouge, est diffusée sur la ligne de test pour exciter et faire émettre une fluorescence aux particules de latex fluorescentes qui sont liées dans la ligne de test dans des tests vrais positifs, pour améliorer la couleur visible au niveau de la ligne de test.
PCT/US2009/046848 2008-06-10 2009-06-10 Test de détection d'analyte visuel/par fluorescence combiné WO2009152209A2 (fr)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US6025808P 2008-06-10 2008-06-10
US61/060,258 2008-06-10
US8087908P 2008-07-15 2008-07-15
US61/080,879 2008-07-15
US9893508P 2008-09-22 2008-09-22
US61/098,935 2008-09-22
US17905909P 2009-05-18 2009-05-18
US61/179,059 2009-05-18

Publications (2)

Publication Number Publication Date
WO2009152209A2 WO2009152209A2 (fr) 2009-12-17
WO2009152209A3 true WO2009152209A3 (fr) 2010-03-11

Family

ID=41417381

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/046848 WO2009152209A2 (fr) 2008-06-10 2009-06-10 Test de détection d'analyte visuel/par fluorescence combiné

Country Status (1)

Country Link
WO (1) WO2009152209A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103293134A (zh) * 2012-03-01 2013-09-11 庞磊 一种荧光分析方法和装置
CN103149182A (zh) * 2011-12-06 2013-06-12 庞磊 一种荧光分析方法和装置
WO2013082943A1 (fr) * 2011-12-06 2013-06-13 Li Jiutong Analyse par fluorescence et dispositif associé
AP2014007834A0 (en) 2012-01-31 2014-07-31 Univ Minnesota Thermal contrast assay and reader
US10816492B2 (en) 2012-01-31 2020-10-27 Regents Of The University Of Minnesota Lateral flow assays with thermal contrast readers
US10725033B2 (en) 2012-01-31 2020-07-28 Regents Of The University Of Minnesota Lateral flow assays with thermal contrast readers
CN103293303B (zh) * 2012-03-01 2016-12-14 上海鑫谱生物科技有限公司 一种荧光分析方法和装置
JP5579343B1 (ja) * 2012-11-28 2014-08-27 古河電気工業株式会社 イムノクロマトグラフィー、これに用いられる検出装置および試薬
ES2620384T3 (es) * 2013-03-07 2017-06-28 Rapid Pathogen Screening Inc. Ensayo de flujo lateral de múltiples planos con zona de desvío
WO2016020492A1 (fr) * 2014-08-06 2016-02-11 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Nouvelle grappe pour la détection d'un analyte
US10808287B2 (en) 2015-10-23 2020-10-20 Rapid Pathogen Screening, Inc. Methods and devices for accurate diagnosis of infections

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6225046B1 (en) * 1995-04-03 2001-05-01 Macquarie Research Ltd. Method for detecting microorganisms
US6335205B1 (en) * 1996-03-13 2002-01-01 Roche Diagnostics Gmbh Method and test strip for determining an analyte
US20030190681A1 (en) * 1998-03-30 2003-10-09 Shafrira Shai Flow cytometer for analysis of general diagnostic factors in cells and body fluids
US20050032244A1 (en) * 2003-08-04 2005-02-10 Shuming Nie Porous materials embedded with nanospecies, methods of fabrication thereof, and methods of use thereof
US20060223192A1 (en) * 2003-09-23 2006-10-05 Smith Henry J Rapid test for glycated albumin
US20070003992A1 (en) * 2003-04-09 2007-01-04 Pentyala Srinivas N Methods and kits for detecting cerebrospinal fluid in a sample
US20080032319A1 (en) * 2005-11-29 2008-02-07 Rnl Bio Co. Ltd. Method for diagnosing colon cancer
US7374950B2 (en) * 2002-11-18 2008-05-20 Princeton Biomeditech Corporation Immunoassay device for diagnosing congestive heart failure and predicting mortality in congestive heart failure patients

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6225046B1 (en) * 1995-04-03 2001-05-01 Macquarie Research Ltd. Method for detecting microorganisms
US6335205B1 (en) * 1996-03-13 2002-01-01 Roche Diagnostics Gmbh Method and test strip for determining an analyte
US20030190681A1 (en) * 1998-03-30 2003-10-09 Shafrira Shai Flow cytometer for analysis of general diagnostic factors in cells and body fluids
US7374950B2 (en) * 2002-11-18 2008-05-20 Princeton Biomeditech Corporation Immunoassay device for diagnosing congestive heart failure and predicting mortality in congestive heart failure patients
US20070003992A1 (en) * 2003-04-09 2007-01-04 Pentyala Srinivas N Methods and kits for detecting cerebrospinal fluid in a sample
US20050032244A1 (en) * 2003-08-04 2005-02-10 Shuming Nie Porous materials embedded with nanospecies, methods of fabrication thereof, and methods of use thereof
US20060223192A1 (en) * 2003-09-23 2006-10-05 Smith Henry J Rapid test for glycated albumin
US20080032319A1 (en) * 2005-11-29 2008-02-07 Rnl Bio Co. Ltd. Method for diagnosing colon cancer

Also Published As

Publication number Publication date
WO2009152209A2 (fr) 2009-12-17

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