WO2009128960A3 - Rapid test including genetic sequence probe - Google Patents

Rapid test including genetic sequence probe Download PDF

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Publication number
WO2009128960A3
WO2009128960A3 PCT/US2009/031011 US2009031011W WO2009128960A3 WO 2009128960 A3 WO2009128960 A3 WO 2009128960A3 US 2009031011 W US2009031011 W US 2009031011W WO 2009128960 A3 WO2009128960 A3 WO 2009128960A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
test
probe
rna
genetic
Prior art date
Application number
PCT/US2009/031011
Other languages
French (fr)
Other versions
WO2009128960A2 (en
Inventor
Weidong Xu
Shyam Mohapatra
Arun Kumar
Original Assignee
Ultrapid Nanodiagnostics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/008,861 external-priority patent/US20090181361A1/en
Application filed by Ultrapid Nanodiagnostics, Inc. filed Critical Ultrapid Nanodiagnostics, Inc.
Priority to EP09732630A priority Critical patent/EP2245186A4/en
Priority to CN2009801086971A priority patent/CN101978072A/en
Priority to US12/424,903 priority patent/US20100105024A1/en
Publication of WO2009128960A2 publication Critical patent/WO2009128960A2/en
Publication of WO2009128960A3 publication Critical patent/WO2009128960A3/en
Priority to ZA2010/05791A priority patent/ZA201005791B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A rapid test kit may have a genetic probe, and antibody detecting probe or a combination of a genetic probe and an antibody detecting probe disposed within one or more test windows of the test kit. A cellulose filter paper membrane with a flow rate selected in a range of about 0.04 to about 0.4 ml/min/cm2 is used in one example, The test kit provides for rapid screening for DNA, RNA or fragments of DNA or RNA in a bodily fluid or antibodies indicating exposure to such DNA/RNA. The genetic probe may include single stranded DNA or a fragment of single stranded DNA, such as primer, immobilized on the filter paper, and a single stranded DNA, such as the same or a different primer, conjugated with a marker, such as a nanotube or nanoparticle. For example, a gold nanoparticle or a carbon nanotube may be used as a staining agent by conjugating the gold nanoparticle or the carbon nanotube to a genetic probe, such as a DNA primer capable of binding with a complementary DNA or viral RNA or a fratment of one of these. By comparing contrast or intensity of a test spot to a standard, a viral load may be reported. By comparing a test region using the genetic marker and a test region using an antigen to detect antibodies, a sensitive and specific test may be conducted during use of a vaccine to determine the effectiveness of the vaccine, for example.
PCT/US2009/031011 2008-01-14 2009-01-14 Rapid test including genetic sequence probe WO2009128960A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP09732630A EP2245186A4 (en) 2008-01-14 2009-01-14 Rapid test including genetic sequence probe
CN2009801086971A CN101978072A (en) 2008-01-14 2009-01-14 Rapid test including genetic sequence probe
US12/424,903 US20100105024A1 (en) 2008-01-14 2009-04-16 Rapid test including genetic sequence probe
ZA2010/05791A ZA201005791B (en) 2008-01-14 2010-08-13 Rapid test including genetic sequence probe

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US12/008,861 US20090181361A1 (en) 2008-01-14 2008-01-14 Rapid test for detecting infection
US12/008,861 2008-01-14
US11893908P 2008-12-01 2008-12-01
US61/118,939 2008-12-01

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US12/008,861 Continuation-In-Part US20090181361A1 (en) 2008-01-14 2008-01-14 Rapid test for detecting infection

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/424,903 Continuation US20100105024A1 (en) 2008-01-14 2009-04-16 Rapid test including genetic sequence probe

Publications (2)

Publication Number Publication Date
WO2009128960A2 WO2009128960A2 (en) 2009-10-22
WO2009128960A3 true WO2009128960A3 (en) 2010-03-11

Family

ID=41199626

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/031011 WO2009128960A2 (en) 2008-01-14 2009-01-14 Rapid test including genetic sequence probe

Country Status (4)

Country Link
EP (1) EP2245186A4 (en)
CN (1) CN101978072A (en)
WO (1) WO2009128960A2 (en)
ZA (1) ZA201005791B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103667534A (en) * 2013-12-12 2014-03-26 东北制药集团辽宁生物医药有限公司 High-accuracy quantitative detection method and kit for HIV-1 nucleic acid

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102102125A (en) * 2010-11-29 2011-06-22 无锡久光纳米生物科技有限公司 Method for detecting DNA sequence through polymerase chain reaction staining technology
EP2808387B1 (en) * 2012-01-25 2017-06-21 Japan Science And Technology Agency Oligonucleotide for hiv detection, hiv detection kit, and hiv detection method
CN103571831A (en) * 2013-10-10 2014-02-12 中国科学技术大学 DNA molecular machine and single-base mutation detection method based on DNA molecular machine as well as application of method
RU2557951C1 (en) * 2014-05-27 2015-07-27 федеральное государственное бюджетное учреждение "Федеральный научно-исследовательский центр эпидемиологии и микробиологии имени почетного академика Н.Ф. Гамалеи" Министерства здравоохранения Российской Федерации (ФГБУ "ФНИЦЭМ им. Н.Ф. Гамалеи" Минздрава России) Method for producing multiplex rickettsial diagnostic preparation
CN105911048B (en) * 2016-04-08 2019-07-09 合肥工业大学 The test paper of carbon nanotube label and quickly detects Hg at preparation method2+Method
CN107418913B (en) * 2017-06-14 2020-12-22 湖南省农业生物技术研究中心 Application of microbial agent for converting heavy metal cadmium in polluted soil
WO2018229733A1 (en) * 2017-06-16 2018-12-20 Beijing Advaccine Biotechnology Co., Ltd. Immuno-biomarkers distinguish responsiveness versus non-responsiveness during immunotherapeutic treatments
CN110082534A (en) * 2019-03-20 2019-08-02 中国农业科学院兰州兽医研究所 A kind of universal testing kit of sheep Babesia
WO2020264210A1 (en) * 2019-06-25 2020-12-30 The Board Of Trustees Of The University Of Illinois Methods and devices for identifying pathogens and antibodies and treatment device therefore
CN110261605B (en) * 2019-06-25 2022-07-15 陕西科技大学 Method for improving detection sensitivity of test paper by modifying chitosan
CN110895283A (en) * 2019-12-10 2020-03-20 宁波奥丞生物科技有限公司 High-sensitivity D-dimer detection kit and use method thereof
CN111948388B (en) * 2020-08-18 2022-03-22 山东农业大学 Colloidal gold test strip for detecting clostridium putrefactive, preparation method and application thereof
CN114836581B (en) * 2022-06-02 2024-03-12 昆明理工大学 Primer combination for detecting pathogens of digestive tract infectious diseases

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541059A (en) * 1993-12-29 1996-07-30 Chu; Albert E. Immunoassay device having an internal control of protein A and methods of using same
US20040002063A1 (en) * 2002-05-16 2004-01-01 Medmira Inc. Rapid vaccinia antibody detection device, method and test kit
US20040086897A1 (en) * 2002-05-07 2004-05-06 Mirkin Chad A. Nanoparticle probes with Raman Spectroscopic fingerprints for analyte detection
US20050037397A1 (en) * 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20050084881A1 (en) * 2003-05-13 2005-04-21 Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10253632A (en) * 1997-03-10 1998-09-25 Nissui Pharm Co Ltd Method, kit and device for analysis
EP1249500A1 (en) * 2001-04-12 2002-10-16 chimera biotec GmbH Method for the determination of an analyte

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541059A (en) * 1993-12-29 1996-07-30 Chu; Albert E. Immunoassay device having an internal control of protein A and methods of using same
US20050037397A1 (en) * 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20040086897A1 (en) * 2002-05-07 2004-05-06 Mirkin Chad A. Nanoparticle probes with Raman Spectroscopic fingerprints for analyte detection
US20040002063A1 (en) * 2002-05-16 2004-01-01 Medmira Inc. Rapid vaccinia antibody detection device, method and test kit
US20050084881A1 (en) * 2003-05-13 2005-04-21 Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2245186A4 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103667534A (en) * 2013-12-12 2014-03-26 东北制药集团辽宁生物医药有限公司 High-accuracy quantitative detection method and kit for HIV-1 nucleic acid

Also Published As

Publication number Publication date
ZA201005791B (en) 2011-10-26
CN101978072A (en) 2011-02-16
WO2009128960A2 (en) 2009-10-22
EP2245186A2 (en) 2010-11-03
EP2245186A4 (en) 2011-08-24

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