WO2009128960A3 - Rapid test including genetic sequence probe - Google Patents

Rapid test including genetic sequence probe

Info

Publication number
WO2009128960A3
WO2009128960A3 PCT/US2009/031011 US2009031011W WO2009128960A3 WO 2009128960 A3 WO2009128960 A3 WO 2009128960A3 US 2009031011 W US2009031011 W US 2009031011W WO 2009128960 A3 WO2009128960 A3 WO 2009128960A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
dna
test
probe
rna
genetic
Prior art date
Application number
PCT/US2009/031011
Other languages
French (fr)
Other versions
WO2009128960A2 (en )
Inventor
Weidong Xu
Shyam Mohapatra
Arun Kumar
Original Assignee
Ultrapid Nanodiagnostics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y02A50/50Chemical or biological analysis of biological material for identifying the disease, e.g. blood or urine testing, rapid diagnostic tests [RTDs] or immunological testing
    • Y02A50/55Chemical or biological analysis of biological material for identifying the disease, e.g. blood or urine testing, rapid diagnostic tests [RTDs] or immunological testing the disease being Leishmaniasis, Sand-fly fever, phlebotomus fever, kala-azar, black fever or Dumdum fever

Abstract

A rapid test kit may have a genetic probe, and antibody detecting probe or a combination of a genetic probe and an antibody detecting probe disposed within one or more test windows of the test kit. A cellulose filter paper membrane with a flow rate selected in a range of about 0.04 to about 0.4 ml/min/cm2 is used in one example, The test kit provides for rapid screening for DNA, RNA or fragments of DNA or RNA in a bodily fluid or antibodies indicating exposure to such DNA/RNA. The genetic probe may include single stranded DNA or a fragment of single stranded DNA, such as primer, immobilized on the filter paper, and a single stranded DNA, such as the same or a different primer, conjugated with a marker, such as a nanotube or nanoparticle. For example, a gold nanoparticle or a carbon nanotube may be used as a staining agent by conjugating the gold nanoparticle or the carbon nanotube to a genetic probe, such as a DNA primer capable of binding with a complementary DNA or viral RNA or a fratment of one of these. By comparing contrast or intensity of a test spot to a standard, a viral load may be reported. By comparing a test region using the genetic marker and a test region using an antigen to detect antibodies, a sensitive and specific test may be conducted during use of a vaccine to determine the effectiveness of the vaccine, for example.
PCT/US2009/031011 2008-01-14 2009-01-14 Rapid test including genetic sequence probe WO2009128960A3 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/008,861 2008-01-14
US12008861 US20090181361A1 (en) 2008-01-14 2008-01-14 Rapid test for detecting infection
US11893908 true 2008-12-01 2008-12-01
US61/118,939 2008-12-01

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20090732630 EP2245186A4 (en) 2008-01-14 2009-01-14 Rapid test including genetic sequence probe
CN 200980108697 CN101978072A (en) 2008-01-14 2009-01-14 Rapid test including genetic sequence probe
US12424903 US20100105024A1 (en) 2008-01-14 2009-04-16 Rapid test including genetic sequence probe

Publications (2)

Publication Number Publication Date
WO2009128960A2 true WO2009128960A2 (en) 2009-10-22
WO2009128960A3 true true WO2009128960A3 (en) 2010-03-11

Family

ID=41199626

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/031011 WO2009128960A3 (en) 2008-01-14 2009-01-14 Rapid test including genetic sequence probe

Country Status (3)

Country Link
EP (1) EP2245186A4 (en)
CN (1) CN101978072A (en)
WO (1) WO2009128960A3 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102102125A (en) * 2010-11-29 2011-06-22 无锡久光纳米生物科技有限公司 Method for detecting DNA sequence through polymerase chain reaction staining technology
EP2808387B1 (en) * 2012-01-25 2017-06-21 Japan Science And Technology Agency Oligonucleotide for hiv detection, hiv detection kit, and hiv detection method
CN103571831A (en) * 2013-10-10 2014-02-12 中国科学技术大学 DNA molecular machine and single-base mutation detection method based on DNA molecular machine as well as application of method
CN103667534B (en) * 2013-12-12 2015-03-25 东北制药集团辽宁生物医药有限公司 High-accuracy quantitative detection method and kit for HIV-1 nucleic acid
RU2557951C1 (en) * 2014-05-27 2015-07-27 федеральное государственное бюджетное учреждение "Федеральный научно-исследовательский центр эпидемиологии и микробиологии имени почетного академика Н.Ф. Гамалеи" Министерства здравоохранения Российской Федерации (ФГБУ "ФНИЦЭМ им. Н.Ф. Гамалеи" Минздрава России) Method for producing multiplex rickettsial diagnostic preparation

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541059A (en) * 1993-12-29 1996-07-30 Chu; Albert E. Immunoassay device having an internal control of protein A and methods of using same
US20040002063A1 (en) * 2002-05-16 2004-01-01 Medmira Inc. Rapid vaccinia antibody detection device, method and test kit
US20040086897A1 (en) * 2002-05-07 2004-05-06 Mirkin Chad A. Nanoparticle probes with Raman Spectroscopic fingerprints for analyte detection
US20050037397A1 (en) * 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20050084881A1 (en) * 2003-05-13 2005-04-21 Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10253632A (en) * 1997-03-10 1998-09-25 Nissui Pharm Co Ltd Method, kit and device for analysis
EP1249500A1 (en) * 2001-04-12 2002-10-16 chimera biotec GmbH Method for the determination of an analyte

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5541059A (en) * 1993-12-29 1996-07-30 Chu; Albert E. Immunoassay device having an internal control of protein A and methods of using same
US20050037397A1 (en) * 2001-03-28 2005-02-17 Nanosphere, Inc. Bio-barcode based detection of target analytes
US20040086897A1 (en) * 2002-05-07 2004-05-06 Mirkin Chad A. Nanoparticle probes with Raman Spectroscopic fingerprints for analyte detection
US20040002063A1 (en) * 2002-05-16 2004-01-01 Medmira Inc. Rapid vaccinia antibody detection device, method and test kit
US20050084881A1 (en) * 2003-05-13 2005-04-21 Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2245186A4 *

Also Published As

Publication number Publication date Type
EP2245186A2 (en) 2010-11-03 application
EP2245186A4 (en) 2011-08-24 application
CN101978072A (en) 2011-02-16 application
WO2009128960A2 (en) 2009-10-22 application

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