WO2007095279A3 - Dual nanoparticle assay for detection and separation of biological species - Google Patents

Dual nanoparticle assay for detection and separation of biological species

Info

Publication number
WO2007095279A3
WO2007095279A3 PCT/US2007/003883 US2007003883W WO2007095279A3 WO 2007095279 A3 WO2007095279 A3 WO 2007095279A3 US 2007003883 W US2007003883 W US 2007003883W WO 2007095279 A3 WO2007095279 A3 WO 2007095279A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
cells
aptamer
detection
nanoparticles
methods
Prior art date
Application number
PCT/US2007/003883
Other languages
French (fr)
Other versions
WO2007095279A2 (en )
Inventor
Weihong Tan
Joshua Elliott Smith
Colin Donnell Medley
Joshua Kenneth Herr
Dihua Shangguan
Original Assignee
Univ Florida
Weihong Tan
Joshua Elliott Smith
Colin Donnell Medley
Joshua Kenneth Herr
Dihua Shangguan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966Animal cells
    • G01N33/56972White blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326Magnetic particles
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407Specifically defined cancers
    • G01N33/57426Specifically defined cancers leukemia
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845Methods of identifying protein-protein interactions in protein mixtures

Abstract

The subject invention provides novel methods, kits, and compositions for use in detecting, quantifying, and/or separating target cells in a sample. In one embodiment, methods for the rapid collection and detection of leukemia cells are provided using a novel dual nanoparticle assay with aptamers as the molecular recognition element. Aptamer sequences are prepared using a cell based SELEX strategy, where the aptamers are specific for CCRF-CEM acute leukemia (AL) cells. Such aptamers preferably demonstrate a high degree of specificity or selectivity for AL cells in complex mixtures, including whole blood samples. According to one embodiment of the invention, aptamer modified magnetic nanoparticles are used for target cell extraction, while aptamer modified fluorescent nanoparticles are simultaneously added for sensitive cell detection. Combining two types of nanoparticle-based screening techniques allows for rapid, selective, and sensitive detection not possible by using either particle alone. In a related embodiment, fluorescent nanoparticles amplify the signal intensity corresponding to a single aptamer binding event, resulting in improved sensitivity over methods using individual dye labeled probes. In addition, aptamer modified magnetic nanoparticles allow for rapid extraction of target cells not possible with other separation methods. Fluorescent imaging and flow cytometry are used for cellular detection to demonstrate the application of this method for medical diagnostics.
PCT/US2007/003883 2006-02-14 2007-02-14 Dual nanoparticle assay for detection and separation of biological species WO2007095279A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US77326706 true 2006-02-14 2006-02-14
US60/773,267 2006-02-14

Publications (2)

Publication Number Publication Date
WO2007095279A2 true WO2007095279A2 (en) 2007-08-23
WO2007095279A3 true true WO2007095279A3 (en) 2007-11-15

Family

ID=38372106

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/003883 WO2007095279A3 (en) 2006-02-14 2007-02-14 Dual nanoparticle assay for detection and separation of biological species

Country Status (1)

Country Link
WO (1) WO2007095279A3 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105120849A (en) * 2013-03-14 2015-12-02 Ran生物技术公司 Methods and materials for detection of biologicals

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008064667B4 (en) 2008-07-15 2011-06-09 Lzh Laserzentrum Hannover E.V. A method for producing a Detektionskonjugats
CN102023147B (en) * 2010-09-29 2012-02-22 江南大学 Method for detecting ochratoxin A by magnetic separation of adapter-functionalized magnetic nano material and marking of up-conversion fluorescent nano material
JP6103778B2 (en) 2011-11-03 2017-03-29 キアジェン ゲイサーズバーグ インコーポレイテッド Cells were fixed by using a carboxylated surface, materials and methods for isolating, and concentrated
CN103940792B (en) * 2014-02-20 2016-06-22 江南大学 A method for converting multi-color fluorescent label detected simultaneously based on three kinds of foodborne pathogens
CN104818279A (en) * 2015-04-23 2015-08-05 河南省农业科学院 Ochratoxin A nucleic acid adapter and application thereof to sample magnetic separation preprocessing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020037506A1 (en) * 2000-04-18 2002-03-28 Yun Lin Aptamer based two-site binding assay
US6777186B2 (en) * 1996-07-29 2004-08-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20040166505A1 (en) * 1999-05-07 2004-08-26 Quantum Dot Corporation Method of detecting an analyte in a sample using semiconductor nanocrystals as a detectable label

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6777186B2 (en) * 1996-07-29 2004-08-17 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US20040166505A1 (en) * 1999-05-07 2004-08-26 Quantum Dot Corporation Method of detecting an analyte in a sample using semiconductor nanocrystals as a detectable label
US20020037506A1 (en) * 2000-04-18 2002-03-28 Yun Lin Aptamer based two-site binding assay

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105120849A (en) * 2013-03-14 2015-12-02 Ran生物技术公司 Methods and materials for detection of biologicals

Also Published As

Publication number Publication date Type
WO2007095279A2 (en) 2007-08-23 application

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